LOCUS       DANKAY010000001        97100 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000001 DANKAY010000000
VERSION     DANKAY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 97100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            591..1280
                     /locus_tag="PWY98_000001"
     CDS             591..1280
                     /locus_tag="PWY98_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2035794.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="PWY98_000002"
     CDS             1267..2475
                     /locus_tag="PWY98_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2035795.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="PWY98_000003"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="PWY98_000003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2035796.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PWY98_000004"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PWY98_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2035797.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="PWY98_000005"
     CDS             complement(6578..7273)
                     /locus_tag="PWY98_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2035798.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PWY98_000006"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PWY98_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2035799.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="PWY98_000007"
     CDS             complement(8472..8663)
                     /locus_tag="PWY98_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2035800.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="PWY98_000008"
     CDS             complement(8684..9574)
                     /locus_tag="PWY98_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2035801.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="PWY98_000009"
     CDS             complement(9561..10328)
                     /locus_tag="PWY98_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2035802.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PWY98_000010"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PWY98_000010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2035803.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PWY98_000011"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PWY98_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2035804.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PWY98_000012"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PWY98_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2035805.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="PWY98_000013"
     CDS             14899..15282
                     /locus_tag="PWY98_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035806.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="PWY98_000014"
     CDS             complement(15450..16208)
                     /locus_tag="PWY98_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2035807.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="PWY98_000015"
     CDS             complement(16226..17044)
                     /locus_tag="PWY98_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2035808.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PWY98_000016"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PWY98_000016"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2035809.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PWY98_000017"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PWY98_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2035810.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="PWY98_000018"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="PWY98_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2035811.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="PWY98_000019"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="PWY98_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2035812.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="PWY98_000020"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="PWY98_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2035813.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="PWY98_000021"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="PWY98_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2035814.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="PWY98_000022"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="PWY98_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2035815.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="PWY98_000023"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="PWY98_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2035816.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="PWY98_000024"
     CDS             27086..27385
                     /locus_tag="PWY98_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2035817.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="PWY98_000025"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="PWY98_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2035818.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="PWY98_000026"
     CDS             27973..28209
                     /locus_tag="PWY98_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2035819.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="PWY98_000027"
     CDS             28352..30325
                     /locus_tag="PWY98_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2035820.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="PWY98_000028"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="PWY98_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2035821.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="PWY98_000029"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="PWY98_000029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2035822.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="PWY98_000030"
     CDS             33204..34496
                     /locus_tag="PWY98_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2035823.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35283..36146
                     /locus_tag="PWY98_000031"
     CDS             35283..36146
                     /locus_tag="PWY98_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2035824.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36275..37897)
                     /locus_tag="PWY98_000032"
     CDS             complement(36275..37897)
                     /locus_tag="PWY98_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2035825.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38146..38667
                     /locus_tag="PWY98_000033"
     CDS             38146..38667
                     /locus_tag="PWY98_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2035826.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="PWY98_000034"
     CDS             complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="PWY98_000034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2035827.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40748..41566
                     /locus_tag="PWY98_000035"
     CDS             40748..41566
                     /locus_tag="PWY98_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2035828.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41669..41866)
                     /locus_tag="PWY98_000036"
     CDS             complement(41669..41866)
                     /locus_tag="PWY98_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035829.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42035..42613
                     /locus_tag="PWY98_000037"
     CDS             42035..42613
                     /locus_tag="PWY98_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2035830.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42708..42845
                     /locus_tag="PWY98_000038"
     CDS             42708..42845
                     /locus_tag="PWY98_000038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035831.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43658..44161)
                     /locus_tag="PWY98_000039"
     CDS             complement(43658..44161)
                     /locus_tag="PWY98_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2035832.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44520..45545
                     /locus_tag="PWY98_000040"
     CDS             44520..45545
                     /locus_tag="PWY98_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2035833.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45868..46746
                     /locus_tag="PWY98_000041"
     CDS             45868..46746
                     /locus_tag="PWY98_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2035834.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47222..47338
                     /locus_tag="PWY98_000042"
     CDS             47222..47338
                     /locus_tag="PWY98_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2035835.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48266..48604
                     /locus_tag="PWY98_000043"
     CDS             48266..48604
                     /locus_tag="PWY98_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035836.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48698..48928
                     /locus_tag="PWY98_000044"
     CDS             48698..48928
                     /locus_tag="PWY98_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035837.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49328..49699
                     /locus_tag="PWY98_000045"
     CDS             49328..49699
                     /locus_tag="PWY98_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2035838.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49973..50665)
                     /locus_tag="PWY98_000046"
     CDS             complement(49973..50665)
                     /locus_tag="PWY98_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2035839.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50668..51453)
                     /locus_tag="PWY98_000047"
     CDS             complement(50668..51453)
                     /locus_tag="PWY98_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2035840.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51690..53090
                     /gene="lacG"
                     /locus_tag="PWY98_000048"
     CDS             51690..53090
                     /gene="lacG"
                     /locus_tag="PWY98_000048"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2035841.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53115..53432
                     /locus_tag="PWY98_000049"
     CDS             53115..53432
                     /locus_tag="PWY98_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2035842.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53445..55118
                     /locus_tag="PWY98_000050"
     CDS             53445..55118
                     /locus_tag="PWY98_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2035843.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55132..56763
                     /locus_tag="PWY98_000051"
     CDS             55132..56763
                     /locus_tag="PWY98_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2035844.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57254..58819)
                     /gene="guaA"
                     /locus_tag="PWY98_000052"
     CDS             complement(57254..58819)
                     /gene="guaA"
                     /locus_tag="PWY98_000052"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2035845.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58878..59801)
                     /locus_tag="PWY98_000053"
     CDS             complement(58878..59801)
                     /locus_tag="PWY98_000053"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2035846.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            59997..60602
                     /locus_tag="PWY98_000054"
     CDS             59997..60602
                     /locus_tag="PWY98_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2035847.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60910..61926
                     /locus_tag="PWY98_000055"
     CDS             60910..61926
                     /locus_tag="PWY98_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2035848.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62067..63368
                     /locus_tag="PWY98_000056"
     CDS             62067..63368
                     /locus_tag="PWY98_000056"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2035849.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63414..64076
                     /gene="deoC"
                     /locus_tag="PWY98_000057"
     CDS             63414..64076
                     /gene="deoC"
                     /locus_tag="PWY98_000057"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2035850.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64097..64495
                     /locus_tag="PWY98_000058"
     CDS             64097..64495
                     /locus_tag="PWY98_000058"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2035851.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64561..65643
                     /locus_tag="PWY98_000059"
     CDS             64561..65643
                     /locus_tag="PWY98_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2035852.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65855..67417
                     /locus_tag="PWY98_000060"
     CDS             65855..67417
                     /locus_tag="PWY98_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2035853.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67410..68543
                     /locus_tag="PWY98_000061"
     CDS             67410..68543
                     /locus_tag="PWY98_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2035854.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68543..69499
                     /locus_tag="PWY98_000062"
     CDS             68543..69499
                     /locus_tag="PWY98_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2035855.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69598..70764
                     /gene="deoB"
                     /locus_tag="PWY98_000063"
     CDS             69598..70764
                     /gene="deoB"
                     /locus_tag="PWY98_000063"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2035856.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70788..71606
                     /locus_tag="PWY98_000064"
     CDS             70788..71606
                     /locus_tag="PWY98_000064"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2035857.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71623..72330
                     /gene="deoD"
                     /locus_tag="PWY98_000065"
     CDS             71623..72330
                     /gene="deoD"
                     /locus_tag="PWY98_000065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2035858.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72628..73314)
                     /gene="gpmA"
                     /locus_tag="PWY98_000066"
     CDS             complement(72628..73314)
                     /gene="gpmA"
                     /locus_tag="PWY98_000066"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2035859.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73486..74067)
                     /locus_tag="PWY98_000067"
     CDS             complement(73486..74067)
                     /locus_tag="PWY98_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2035860.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74200..74883)
                     /gene="rpiA"
                     /locus_tag="PWY98_000068"
     CDS             complement(74200..74883)
                     /gene="rpiA"
                     /locus_tag="PWY98_000068"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2035861.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75202..75615
                     /gene="rpsL"
                     /locus_tag="PWY98_000069"
     CDS             75202..75615
                     /gene="rpsL"
                     /locus_tag="PWY98_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2035862.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75699..76169
                     /gene="rpsG"
                     /locus_tag="PWY98_000070"
     CDS             75699..76169
                     /gene="rpsG"
                     /locus_tag="PWY98_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2035863.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76263..78350
                     /gene="fusA"
                     /locus_tag="PWY98_000071"
     CDS             76263..78350
                     /gene="fusA"
                     /locus_tag="PWY98_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2035864.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78522..79709
                     /gene="tuf"
                     /locus_tag="PWY98_000072"
     CDS             78522..79709
                     /gene="tuf"
                     /locus_tag="PWY98_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2035865.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80116..80424
                     /gene="rpsJ"
                     /locus_tag="PWY98_000073"
     CDS             80116..80424
                     /gene="rpsJ"
                     /locus_tag="PWY98_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2035866.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80455..81084
                     /gene="rplC"
                     /locus_tag="PWY98_000074"
     CDS             80455..81084
                     /gene="rplC"
                     /locus_tag="PWY98_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2035867.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81112..81735
                     /gene="rplD"
                     /locus_tag="PWY98_000075"
     CDS             81112..81735
                     /gene="rplD"
                     /locus_tag="PWY98_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2035868.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81735..82025
                     /gene="rplW"
                     /locus_tag="PWY98_000076"
     CDS             81735..82025
                     /gene="rplW"
                     /locus_tag="PWY98_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2035869.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82064..82897
                     /gene="rplB"
                     /locus_tag="PWY98_000077"
     CDS             82064..82897
                     /gene="rplB"
                     /locus_tag="PWY98_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2035870.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82940..83218
                     /gene="rpsS"
                     /locus_tag="PWY98_000078"
     CDS             82940..83218
                     /gene="rpsS"
                     /locus_tag="PWY98_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2035871.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83241..83588
                     /gene="rplV"
                     /locus_tag="PWY98_000079"
     CDS             83241..83588
                     /gene="rplV"
                     /locus_tag="PWY98_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2035872.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83602..84258
                     /gene="rpsC"
                     /locus_tag="PWY98_000080"
     CDS             83602..84258
                     /gene="rpsC"
                     /locus_tag="PWY98_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2035873.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84261..84695
                     /gene="rplP"
                     /locus_tag="PWY98_000081"
     CDS             84261..84695
                     /gene="rplP"
                     /locus_tag="PWY98_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2035874.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84685..84873
                     /gene="rpmC"
                     /locus_tag="PWY98_000082"
     CDS             84685..84873
                     /gene="rpmC"
                     /locus_tag="PWY98_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2035875.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84898..85164
                     /gene="rpsQ"
                     /locus_tag="PWY98_000083"
     CDS             84898..85164
                     /gene="rpsQ"
                     /locus_tag="PWY98_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2035876.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85222..85590
                     /gene="rplN"
                     /locus_tag="PWY98_000084"
     CDS             85222..85590
                     /gene="rplN"
                     /locus_tag="PWY98_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2035877.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85627..85935
                     /gene="rplX"
                     /locus_tag="PWY98_000085"
     CDS             85627..85935
                     /gene="rplX"
                     /locus_tag="PWY98_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2035878.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85963..86502
                     /gene="rplE"
                     /locus_tag="PWY98_000086"
     CDS             85963..86502
                     /gene="rplE"
                     /locus_tag="PWY98_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2035879.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86521..86706
                     /locus_tag="PWY98_000087"
     CDS             86521..86706
                     /locus_tag="PWY98_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2035880.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86742..87140
                     /gene="rpsH"
                     /locus_tag="PWY98_000088"
     CDS             86742..87140
                     /gene="rpsH"
                     /locus_tag="PWY98_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2035881.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87172..87708
                     /gene="rplF"
                     /locus_tag="PWY98_000089"
     CDS             87172..87708
                     /gene="rplF"
                     /locus_tag="PWY98_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2035882.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87869..88225
                     /gene="rplR"
                     /locus_tag="PWY98_000090"
     CDS             87869..88225
                     /gene="rplR"
                     /locus_tag="PWY98_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2035883.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88246..88746
                     /gene="rpsE"
                     /locus_tag="PWY98_000091"
     CDS             88246..88746
                     /gene="rpsE"
                     /locus_tag="PWY98_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2035884.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88761..88940
                     /gene="rpmD"
                     /locus_tag="PWY98_000092"
     CDS             88761..88940
                     /gene="rpmD"
                     /locus_tag="PWY98_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2035885.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88984..89424
                     /gene="rplO"
                     /locus_tag="PWY98_000093"
     CDS             88984..89424
                     /gene="rplO"
                     /locus_tag="PWY98_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2035886.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89424..90719
                     /gene="secY"
                     /locus_tag="PWY98_000094"
     CDS             89424..90719
                     /gene="secY"
                     /locus_tag="PWY98_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2035887.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90781..91428
                     /locus_tag="PWY98_000095"
     CDS             90781..91428
                     /locus_tag="PWY98_000095"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2035888.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91619..91837
                     /gene="infA"
                     /locus_tag="PWY98_000096"
     CDS             91619..91837
                     /gene="infA"
                     /locus_tag="PWY98_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2035889.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91870..91986
                     /gene="rpmJ"
                     /locus_tag="PWY98_000097"
     CDS             91870..91986
                     /gene="rpmJ"
                     /locus_tag="PWY98_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2035890.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92004..92369
                     /gene="rpsM"
                     /locus_tag="PWY98_000098"
     CDS             92004..92369
                     /gene="rpsM"
                     /locus_tag="PWY98_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2035891.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92397..92786
                     /gene="rpsK"
                     /locus_tag="PWY98_000099"
     CDS             92397..92786
                     /gene="rpsK"
                     /locus_tag="PWY98_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2035892.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92866..93804
                     /locus_tag="PWY98_000100"
     CDS             92866..93804
                     /locus_tag="PWY98_000100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2035893.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93832..94212
                     /gene="rplQ"
                     /locus_tag="PWY98_000101"
     CDS             93832..94212
                     /gene="rplQ"
                     /locus_tag="PWY98_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2035894.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94484..95323
                     /locus_tag="PWY98_000102"
     CDS             94484..95323
                     /locus_tag="PWY98_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2035895.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95299..96168
                     /locus_tag="PWY98_000103"
     CDS             95299..96168
                     /locus_tag="PWY98_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2035896.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96158..96955
                     /locus_tag="PWY98_000104"
     CDS             96158..96955
                     /locus_tag="PWY98_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2035897.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        30319 a        17927 c        20128 g        28726 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34741 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34801 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34861 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34921 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34981 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    35041 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    35101 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    35161 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    35221 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35281 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35341 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35401 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35461 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35521 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35581 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35641 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35701 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35761 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35821 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35881 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35941 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    36001 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    36061 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    36121 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    36181 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36241 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36301 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36361 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36421 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36481 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36541 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36601 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36661 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36721 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36781 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36841 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36901 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36961 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    37021 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    37081 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    37141 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    37201 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37261 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37321 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37381 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37441 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37501 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37561 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37621 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37681 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37741 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37801 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37861 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37921 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37981 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    38041 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    38101 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    38161 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    38221 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38281 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38341 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
    38401 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38461 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38521 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38581 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38641 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38701 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38761 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38821 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38881 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38941 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    39001 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    39061 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    39121 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    39181 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39241 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39301 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39361 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39421 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39481 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39541 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39601 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39661 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39721 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39781 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39841 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39901 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39961 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    40021 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    40081 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    40141 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    40201 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40261 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40321 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40381 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40441 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40501 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40561 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40621 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40681 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40741 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40801 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40861 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40921 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40981 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    41041 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    41101 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    41161 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    41221 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41281 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41341 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41401 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41461 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41521 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
    41581 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
    41641 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
    41701 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
    41761 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
    41821 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
    41881 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
    41941 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
    42001 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
    42061 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
    42121 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
    42181 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
    42241 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
    42301 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
    42361 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
    42421 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
    42481 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
    42541 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
    42601 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
    42661 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
    42721 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
    42781 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
    42841 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
    42901 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
    42961 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
    43021 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
    43081 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
    43141 tatctcattt tacacttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
    43201 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
    43261 aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag
    43321 aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat
    43381 atacctgagt agggtataaa ttaagacgga attcctttct agattgagat attgactagg
    43441 aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg
    43501 aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga
    43561 gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt
    43621 ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg
    43681 tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta
    43741 gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta
    43801 tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata
    43861 ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa
    43921 aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt
    43981 tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga
    44041 gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata
    44101 aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat acataccgca
    44161 tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag
    44221 tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt ttttgttggg
    44281 aatccggttt ctttaacaaa gctaagatag cgagttgctt ggaaaagttg ctatcttttt
    44341 tctgtcaaaa cgactcggaa tgttcgtttt ttgttctaat ttctccaagt tgtttacttt
    44401 ttgatactaa gtttatgaaa aatcaagaaa tagttgttat actaggtaat gctgacaaac
    44461 gggattggca tttcatcttg gttcagcttt ttttccaatt actaggaagg agggatagat
    44521 tgttcaagac ttattttcgt ttcaataaaa tacaagataa tccttttcgt atcatgaatc
    44581 tgatgctact tatctttatt gtattatggt taggttacat aaatcatgat tttttgattt
    44641 caagtttttc atcattaggg atctttactt ttttctatta tcaaaacata ccgatgaaat
    44701 atttgctgaa acgtatgttt ctgatcggtt gcggactgtt ggttgctttc gttttgggga
    44761 tcttatcgac ttatgtttta tggcttgaac catttgccgt agctttggtt gctttttcca
    44821 gcagattcgt gttgcgatta ttccatattt ctaaaccagg tggcttgttt tttgcgatgc
    44881 tttctgccat gggaacgagc atgcagctgc caatcgctca attaccgatc gtctcacttt
    44941 atttttttat gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa ttactggatt
    45001 cacggcctga gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt
    45061 tagtgatcat tgattctgtt ttctacagcg ctgccttatt tctttccgtt tatgtcagtc
    45121 acggcttgaa tctgcataat ccttattggt tgaccttgtc atgtgcatcg attttgctag
    45181 cagaaaactt ggatgcgatg aagcatcgac aagtccaata tctgattgga tcgatgggtg
    45241 ggttatgtgt gtcggcattc ttgtcatttg tgccgtttac gcagttgcag accatttttc
    45301 tgatcacttt tctatatgga atcgcacaat ttctagttgc tagaaattat gcagttgcta
    45361 atatttttct taatccgatg gcgttgatgc tatctacact tattcgtgga gcatacttga
    45421 tttctttgat cgaatatcgt tttttaggga tcgtcatagg aagtttcatc ggtttaggtg
    45481 ttgcgtgggt aatgacggtt ggtttgcaac attatttaac ggttgttcgt caaaaattag
    45541 aatagaagga caaatatatg tgccaaaaga ggttatgaca agatttttgt cacaacctct
    45601 aattcaaggt gttcaaaagc cagattctca gcattaagtc aaattcacac aaaaatgtgg
    45661 aaaggaactt tcaaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta
    45721 actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt
    45781 cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg
    45841 gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg
    45901 atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc
    45961 gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat
    46021 agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc
    46081 tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg
    46141 aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct
    46201 tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt
    46261 ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa
    46321 atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct
    46381 cttcatattc cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt
    46441 gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat
    46501 acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt
    46561 tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg
    46621 atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga
    46681 caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact
    46741 tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt
    46801 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    46861 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
    46921 taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata
    46981 ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta
    47041 gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc
    47101 gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt
    47161 aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca
    47221 tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac
    47281 acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga
    47341 agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg
    47401 gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa
    47461 attggtagaa tctttctttt caaattgata ccattcccta attccagtta tatagttaac
    47521 tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc
    47581 ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc
    47641 gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga
    47701 aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg
    47761 tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt
    47821 tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa
    47881 agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat
    47941 caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta
    48001 gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt
    48061 aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag
    48121 tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga
    48181 taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc
    48241 tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa
    48301 cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc
    48361 attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta
    48421 cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac
    48481 accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac
    48541 tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat
    48601 gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca
    48661 caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga
    48721 aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt
    48781 caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat
    48841 agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt
    48901 tttggattat gttagtatgc aacagtaagg gagttcttat tatgaaaaat tttgacacat
    48961 attatgttat tgttttttcg atatctttgc tgttaatatt actaacttta atgcagtata
    49021 aagagtatcc agagtatttt attttaactt ttgtatattt agtaagtagc tacacacttc
    49081 tatataaatt cagaagttct tctagagttt ctaaagtttg tttcttaatt agcttattga
    49141 ttctataata tttttgattt atataggaaa ttaaaaatat tatagaatca acactcagag
    49201 aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc ctggttggga
    49261 aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga
    49321 taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc
    49381 attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat
    49441 tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg
    49501 catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac
    49561 ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat
    49621 ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga
    49681 actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac
    49741 tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa
    49801 ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg
    49861 ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta
    49921 taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat
    49981 taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat
    50041 ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa
    50101 taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa
    50161 caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt
    50221 gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg
    50281 ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg
    50341 ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact
    50401 gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca
    50461 atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat
    50521 tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat
    50581 catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt
    50641 tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc
    50701 agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg
    50761 atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt
    50821 gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg
    50881 tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact
    50941 gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa
    51001 tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg
    51061 agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa
    51121 atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg
    51181 aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt
    51241 ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac
    51301 acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga
    51361 aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc
    51421 tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat
    51481 ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt
    51541 ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt
    51601 tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa
    51661 actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg
    51721 gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg
    51781 ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt
    51841 tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa
    51901 gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag
    51961 gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg
    52021 tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc
    52081 gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag
    52141 tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag
    52201 gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata
    52261 tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa
    52321 ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg gaagataagc
    52381 atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct acctatttag
    52441 gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca aatcaggcca
    52501 gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg cgtaatgact
    52561 acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa
    52621 tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag ggaatcggag
    52681 aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt tatccagaag
    52741 gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac aaaatctata
    52801 tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt atggatcagc
    52861 cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata acagccgggg
    52921 taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg acaaatgggt
    52981 ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt tacccaaaag
    53041 aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa taagtgataa
    53101 tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag atcgttgcat
    53161 attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga caaggaaact
    53221 ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt
    53281 cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg ggattcattt
    53341 ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg attcaagact
    53401 ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat ggaatcatta
    53461 aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt tatttaggag
    53521 ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc attttcattt
    53581 tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc tccacatggc
    53641 tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca gcaaccactg
    53701 ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta atcaacacca
    53761 cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag
    53821 gtggattttc aacggaatat atggggacaa agggactact ggcttccttt gtcgctgcat
    53881 ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt aaaatgccta
    53941 aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc tctttttcag
    54001 tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc tcatttgcag
    54061 aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac ttaggaatcg
    54121 ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg
    54181 tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg caattgttca
    54241 attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt gtaggaacta
    54301 tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt gctaagtcta
    54361 agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct gtcaacgaac
    54421 cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca tttttgttaa
    54481 cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc atgaacagct
    54541 ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta ggaacgggca
    54601 tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc
    54661 taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa aaagcagagg
    54721 tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct gtggcagcga
    54781 gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag
    54841 ggacaagtgg gatgttagct aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga
    54901 tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat tttgatatgg
    54961 tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac accgatcgct
    55021 taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg agagatcctg
    55081 aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat aatgaaaatg
    55141 ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt gggggctgtt
    55201 tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat ttcgatcctg
    55261 tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa agatgcctta
    55321 gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca ggatccttat
    55381 aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac gattgacctt
    55441 gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta cagtgatttt
    55501 tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac ctttgaagaa
    55561 atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa gcagctggac
    55621 gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct ctggccttat
    55681 ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt tttgaagtct
    55741 ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct ccacttagct
    55801 gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag tcgaggtgtg
    55861 tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat gaatgaactt
    55921 caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt agttgaaaca
    55981 gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg tgaagccgaa
    56041 gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt gctggatctt
    56101 gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta ttgggaacct
    56161 ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta tctcagtgtt
    56221 caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc tggaaacgcc
    56281 ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt taaaaatgca
    56341 agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa
    56401 ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga ctacaaacta
    56461 actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag tggcataagc
    56521 aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga tactaatcag
    56581 ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa aacaagtgaa
    56641 tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga aattggggtt
    56701 tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa
    56761 tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat cgttagcgta
    56821 gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa cgatttccag
    56881 tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat tatgctaaag
    56941 agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg cagataacat
    57001 aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt tgagcactta
    57061 gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct ttttatgata
    57121 agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat cttattttta
    57181 atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc cccctccata
    57241 ttatgttggc tctttattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
    57301 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
    57361 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
    57421 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
    57481 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
    57541 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
    57601 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
    57661 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
    57721 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
    57781 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
    57841 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
    57901 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
    57961 ttgcattgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
    58021 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
    58081 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
    58141 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
    58201 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
    58261 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
    58321 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
    58381 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
    58441 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
    58501 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
    58561 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
    58621 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
    58681 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
    58741 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
    58801 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
    58861 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
    58921 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
    58981 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
    59041 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
    59101 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
    59161 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
    59221 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
    59281 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
    59341 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
    59401 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
    59461 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
    59521 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
    59581 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
    59641 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
    59701 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
    59761 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
    59821 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
    59881 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
    59941 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
    60001 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
    60061 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
    60121 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
    60181 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
    60241 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
    60301 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
    60361 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
    60421 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
    60481 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
    60541 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
    60601 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
    60661 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
    60721 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
    60781 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
    60841 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
    60901 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
    60961 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
    61021 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
    61081 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
    61141 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
    61201 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
    61261 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
    61321 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
    61381 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
    61441 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
    61501 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
    61561 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
    61621 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
    61681 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
    61741 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
    61801 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
    61861 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
    61921 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
    61981 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
    62041 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
    62101 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
    62161 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
    62221 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
    62281 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
    62341 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
    62401 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
    62461 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
    62521 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
    62581 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
    62641 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
    62701 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
    62761 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
    62821 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
    62881 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
    62941 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    63001 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    63061 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    63121 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    63181 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    63241 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    63301 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    63361 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    63421 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    63481 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    63541 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    63601 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    63661 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    63721 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    63781 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    63841 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    63901 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    63961 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    64021 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    64081 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    64141 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    64201 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    64261 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    64321 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    64381 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    64441 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    64501 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    64561 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    64621 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    64681 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    64741 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    64801 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    64861 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    64921 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    64981 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    65041 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    65101 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    65161 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    65221 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    65281 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    65341 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    65401 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    65461 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    65521 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    65581 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    65641 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    65701 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    65761 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    65821 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    65881 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    65941 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    66001 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    66061 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    66121 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    66181 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    66241 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    66301 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    66361 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    66421 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    66481 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    66541 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    66601 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    66661 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    66721 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    66781 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    66841 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    66901 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    66961 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    67021 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    67081 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    67141 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    67201 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    67261 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    67321 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    67381 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    67441 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    67501 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    67561 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    67621 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    67681 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    67741 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    67801 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    67861 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    67921 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    67981 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    68041 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    68101 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    68161 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    68221 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    68281 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    68341 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    68401 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    68461 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    68521 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    68581 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    68641 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    68701 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    68761 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    68821 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    68881 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    68941 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    69001 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    69061 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    69121 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    69181 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    69241 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    69301 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    69361 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    69421 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    69481 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    69541 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    69601 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    69661 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    69721 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    69781 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    69841 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    69901 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    69961 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    70021 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    70081 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    70141 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    70201 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    70261 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    70321 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    70381 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    70441 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    70501 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    70561 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    70621 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    70681 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    70741 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    70801 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    70861 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    70921 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    70981 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    71041 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    71101 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    71161 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    71221 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    71281 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    71341 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    71401 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    71461 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    71521 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    71581 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    71641 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    71701 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    71761 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    71821 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    71881 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    71941 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    72001 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    72061 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    72121 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    72181 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    72241 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    72301 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    72361 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    72421 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    72481 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    72541 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    72601 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    72661 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    72721 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    72781 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    72841 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    72901 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    72961 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    73021 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    73081 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    73141 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    73201 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    73261 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    73321 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    73381 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    73441 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    73501 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    73561 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    73621 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    73681 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    73741 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    73801 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    73861 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    73921 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    73981 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    74041 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    74101 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    74161 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    74221 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    74281 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    74341 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    74401 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    74461 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    74521 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    74581 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    74641 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    74701 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    74761 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    74821 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    74881 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    74941 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    75001 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    75061 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    75121 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    75181 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    75241 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    75301 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    75361 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    75421 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    75481 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    75541 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    75601 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    75661 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    75721 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    75781 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    75841 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    75901 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    75961 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    76021 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    76081 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    76141 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    76201 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    76261 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    76321 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    76381 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    76441 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    76501 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    76561 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    76621 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    76681 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    76741 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    76801 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    76861 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    76921 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    76981 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    77041 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    77101 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    77161 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    77221 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    77281 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    77341 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    77401 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    77461 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    77521 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    77581 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    77641 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    77701 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    77761 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    77821 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    77881 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    77941 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    78001 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    78061 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    78121 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    78181 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    78241 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    78301 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttgaaa
    78361 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    78421 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    78481 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    78541 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    78601 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    78661 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    78721 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    78781 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    78841 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    78901 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    78961 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    79021 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    79081 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    79141 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    79201 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    79261 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    79321 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    79381 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    79441 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    79501 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    79561 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    79621 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    79681 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    79741 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    79801 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    79861 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    79921 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    79981 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    80041 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    80101 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    80161 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    80221 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    80281 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    80341 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    80401 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    80461 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    80521 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    80581 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    80641 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    80701 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    80761 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    80821 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    80881 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    80941 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    81001 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    81061 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    81121 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    81181 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    81241 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    81301 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    81361 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    81421 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    81481 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    81541 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    81601 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    81661 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    81721 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    81781 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    81841 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    81901 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    81961 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    82021 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    82081 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    82141 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    82201 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    82261 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    82321 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    82381 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    82441 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    82501 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    82561 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    82621 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    82681 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    82741 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    82801 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    82861 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    82921 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    82981 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    83041 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    83101 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    83161 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    83221 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    83281 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    83341 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    83401 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    83461 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    83521 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    83581 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    83641 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    83701 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    83761 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    83821 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    83881 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    83941 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    84001 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    84061 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    84121 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    84181 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    84241 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    84301 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    84361 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    84421 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    84481 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    84541 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    84601 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    84661 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    84721 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    84781 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    84841 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    84901 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    84961 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    85021 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    85081 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    85141 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    85201 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    85261 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    85321 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    85381 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    85441 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    85501 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    85561 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    85621 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    85681 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    85741 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    85801 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    85861 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    85921 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    85981 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    86041 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    86101 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    86161 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    86221 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    86281 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    86341 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    86401 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    86461 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    86521 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    86581 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    86641 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    86701 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    86761 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    86821 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    86881 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    86941 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    87001 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    87061 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    87121 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    87181 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    87241 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    87301 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    87361 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    87421 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    87481 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    87541 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    87601 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    87661 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    87721 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    87781 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    87841 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    87901 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    87961 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    88021 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    88081 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    88141 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    88201 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    88261 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    88321 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    88381 gtaggatttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    88441 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    88501 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    88561 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    88621 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    88681 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    88741 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    88801 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    88861 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    88921 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    88981 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    89041 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    89101 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    89161 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    89221 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    89281 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    89341 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    89401 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    89461 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    89521 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    89581 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    89641 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    89701 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    89761 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    89821 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    89881 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    89941 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    90001 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    90061 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    90121 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    90181 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    90241 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    90301 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    90361 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    90421 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    90481 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    90541 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    90601 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    90661 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    90721 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    90781 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    90841 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    90901 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    90961 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    91021 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    91081 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    91141 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    91201 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    91261 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    91321 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    91381 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    91441 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    91501 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    91561 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    91621 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    91681 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    91741 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    91801 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    91861 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    91921 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    91981 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    92041 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    92101 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    92161 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    92221 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    92281 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    92341 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    92401 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    92461 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    92521 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    92581 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    92641 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    92701 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    92761 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    92821 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    92881 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    92941 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    93001 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    93061 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    93121 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    93181 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    93241 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    93301 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    93361 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    93421 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    93481 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    93541 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    93601 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    93661 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    93721 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    93781 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    93841 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    93901 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    93961 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    94021 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    94081 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    94141 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    94201 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    94261 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    94321 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    94381 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    94441 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    94501 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    94561 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    94621 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    94681 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatggt
    94741 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    94801 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    94861 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    94921 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    94981 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    95041 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    95101 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    95161 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    95221 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    95281 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    95341 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    95401 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    95461 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    95521 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    95581 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    95641 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    95701 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    95761 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    95821 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    95881 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    95941 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    96001 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    96061 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    96121 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    96181 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    96241 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    96301 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    96361 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    96421 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    96481 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    96541 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    96601 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    96661 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    96721 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    96781 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    96841 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    96901 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    96961 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    97021 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    97081 tgaaaagaaa gaacgaaaaa
//
LOCUS       DANKAY010000002        82603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000002 DANKAY010000000
VERSION     DANKAY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(125..1228)
                     /locus_tag="PWY98_000105"
     CDS             complement(125..1228)
                     /locus_tag="PWY98_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2035898.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1450..1920)
                     /locus_tag="PWY98_000106"
     CDS             complement(1450..1920)
                     /locus_tag="PWY98_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2035899.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2177..3496
                     /locus_tag="PWY98_000107"
     CDS             2177..3496
                     /locus_tag="PWY98_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDL2035900.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3685..4086
                     /locus_tag="PWY98_000108"
                     /pseudo
     CDS             3685..4086
                     /locus_tag="PWY98_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            complement(4391..4705)
                     /locus_tag="PWY98_000109"
                     /pseudo
     CDS             complement(4391..4705)
                     /locus_tag="PWY98_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(4959..6594)
                     /locus_tag="PWY98_000110"
                     /pseudo
     CDS             complement(4959..6594)
                     /locus_tag="PWY98_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6917..7396)
                     /gene="rlmH"
                     /locus_tag="PWY98_000111"
     CDS             complement(6917..7396)
                     /gene="rlmH"
                     /locus_tag="PWY98_000111"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2035901.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            7824..9113
                     /locus_tag="PWY98_000112"
     CDS             7824..9113
                     /locus_tag="PWY98_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2035902.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(9152..9757)
                     /locus_tag="PWY98_000113"
     CDS             complement(9152..9757)
                     /locus_tag="PWY98_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2035903.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(9962..10426)
                     /locus_tag="PWY98_000114"
     CDS             complement(9962..10426)
                     /locus_tag="PWY98_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2035904.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(10448..10768)
                     /locus_tag="PWY98_000115"
     CDS             complement(10448..10768)
                     /locus_tag="PWY98_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2035905.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(10912..11991)
                     /gene="pepA"
                     /locus_tag="PWY98_000116"
     CDS             complement(10912..11991)
                     /gene="pepA"
                     /locus_tag="PWY98_000116"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2035906.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            12191..12541
                     /locus_tag="PWY98_000117"
     CDS             12191..12541
                     /locus_tag="PWY98_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2035907.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12764..13711
                     /locus_tag="PWY98_000118"
     CDS             12764..13711
                     /locus_tag="PWY98_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2035908.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(13768..15186)
                     /gene="pepV"
                     /locus_tag="PWY98_000119"
     CDS             complement(13768..15186)
                     /gene="pepV"
                     /locus_tag="PWY98_000119"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2035909.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            15335..16180
                     /locus_tag="PWY98_000120"
     CDS             15335..16180
                     /locus_tag="PWY98_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2035910.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            16184..16624
                     /locus_tag="PWY98_000121"
     CDS             16184..16624
                     /locus_tag="PWY98_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2035911.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            16674..16868
                     /locus_tag="PWY98_000122"
     CDS             16674..16868
                     /locus_tag="PWY98_000122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035912.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            17004..17690
                     /locus_tag="PWY98_000123"
     CDS             17004..17690
                     /locus_tag="PWY98_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2035913.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(18098..18787)
                     /locus_tag="PWY98_000124"
     CDS             complement(18098..18787)
                     /locus_tag="PWY98_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035914.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(18762..20891)
                     /locus_tag="PWY98_000125"
     CDS             complement(18762..20891)
                     /locus_tag="PWY98_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2035915.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(20939..22276)
                     /locus_tag="PWY98_000126"
     CDS             complement(20939..22276)
                     /locus_tag="PWY98_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2035916.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(22528..22887)
                     /gene="rplT"
                     /locus_tag="PWY98_000127"
     CDS             complement(22528..22887)
                     /gene="rplT"
                     /locus_tag="PWY98_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2035917.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(22940..23140)
                     /gene="rpmI"
                     /locus_tag="PWY98_000128"
     CDS             complement(22940..23140)
                     /gene="rpmI"
                     /locus_tag="PWY98_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2035918.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(23212..23736)
                     /gene="infC"
                     /locus_tag="PWY98_000129"
     CDS             complement(23212..23736)
                     /gene="infC"
                     /locus_tag="PWY98_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2035919.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(23780..23905)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            24056..24688
                     /locus_tag="PWY98_000130"
     CDS             24056..24688
                     /locus_tag="PWY98_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2035920.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(24690..25595)
                     /locus_tag="PWY98_000131"
     CDS             complement(24690..25595)
                     /locus_tag="PWY98_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2035921.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25680..26339)
                     /locus_tag="PWY98_000132"
     CDS             complement(25680..26339)
                     /locus_tag="PWY98_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2035922.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            26655..27599
                     /gene="mvk"
                     /locus_tag="PWY98_000133"
     CDS             26655..27599
                     /gene="mvk"
                     /locus_tag="PWY98_000133"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2035923.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            27592..28569
                     /gene="mvaD"
                     /locus_tag="PWY98_000134"
     CDS             27592..28569
                     /gene="mvaD"
                     /locus_tag="PWY98_000134"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2035924.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            28582..29667
                     /locus_tag="PWY98_000135"
     CDS             28582..29667
                     /locus_tag="PWY98_000135"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2035925.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            29664..30728
                     /locus_tag="PWY98_000136"
     CDS             29664..30728
                     /locus_tag="PWY98_000136"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2035926.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(30977..31780)
                     /locus_tag="PWY98_000137"
     CDS             complement(30977..31780)
                     /locus_tag="PWY98_000137"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2035927.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(31800..32642)
                     /gene="kduI"
                     /locus_tag="PWY98_000138"
     CDS             complement(31800..32642)
                     /gene="kduI"
                     /locus_tag="PWY98_000138"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2035928.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(32658..33665)
                     /locus_tag="PWY98_000139"
     CDS             complement(32658..33665)
                     /locus_tag="PWY98_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2035929.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(33672..34316)
                     /locus_tag="PWY98_000140"
     CDS             complement(33672..34316)
                     /locus_tag="PWY98_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2035930.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(34416..35174)
                     /locus_tag="PWY98_000141"
     CDS             complement(34416..35174)
                     /locus_tag="PWY98_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2035931.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(35301..36452)
                     /gene="fucO"
                     /locus_tag="PWY98_000142"
     CDS             complement(35301..36452)
                     /gene="fucO"
                     /locus_tag="PWY98_000142"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2035932.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(36534..36845)
                     /gene="rhaM"
                     /locus_tag="PWY98_000143"
     CDS             complement(36534..36845)
                     /gene="rhaM"
                     /locus_tag="PWY98_000143"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2035933.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(36847..37692)
                     /gene="rhaD"
                     /locus_tag="PWY98_000144"
     CDS             complement(36847..37692)
                     /gene="rhaD"
                     /locus_tag="PWY98_000144"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2035934.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(37706..38962)
                     /gene="rhaA"
                     /locus_tag="PWY98_000145"
     CDS             complement(37706..38962)
                     /gene="rhaA"
                     /locus_tag="PWY98_000145"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2035935.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(38959..40419)
                     /gene="rhaB"
                     /locus_tag="PWY98_000146"
     CDS             complement(38959..40419)
                     /gene="rhaB"
                     /locus_tag="PWY98_000146"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2035936.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            40572..41441
                     /locus_tag="PWY98_000147"
     CDS             40572..41441
                     /locus_tag="PWY98_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2035937.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(41567..42466)
                     /locus_tag="PWY98_000148"
     CDS             complement(41567..42466)
                     /locus_tag="PWY98_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2035938.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(42631..43455)
                     /locus_tag="PWY98_000149"
     CDS             complement(42631..43455)
                     /locus_tag="PWY98_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2035939.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(43466..44761)
                     /locus_tag="PWY98_000150"
     CDS             complement(43466..44761)
                     /locus_tag="PWY98_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2035940.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(44777..45256)
                     /locus_tag="PWY98_000151"
     CDS             complement(44777..45256)
                     /locus_tag="PWY98_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2035941.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(45262..46290)
                     /locus_tag="PWY98_000152"
     CDS             complement(45262..46290)
                     /locus_tag="PWY98_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2035942.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(46307..47413)
                     /locus_tag="PWY98_000153"
     CDS             complement(46307..47413)
                     /locus_tag="PWY98_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2035943.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(47642..50239)
                     /gene="adhE"
                     /locus_tag="PWY98_000154"
                     /gene_synonym="adhC"
     CDS             complement(47642..50239)
                     /gene="adhE"
                     /locus_tag="PWY98_000154"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2035944.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(50544..50924)
                     /gene="yajC"
                     /locus_tag="PWY98_000155"
     CDS             complement(50544..50924)
                     /gene="yajC"
                     /locus_tag="PWY98_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2035945.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(51021..52166)
                     /gene="tgt"
                     /locus_tag="PWY98_000156"
     CDS             complement(51021..52166)
                     /gene="tgt"
                     /locus_tag="PWY98_000156"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2035946.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            52384..53082
                     /locus_tag="PWY98_000157"
     CDS             52384..53082
                     /locus_tag="PWY98_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2035947.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(53250..55889)
                     /locus_tag="PWY98_000158"
     CDS             complement(53250..55889)
                     /locus_tag="PWY98_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2035948.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(56508..57539)
                     /gene="queA"
                     /locus_tag="PWY98_000159"
     CDS             complement(56508..57539)
                     /gene="queA"
                     /locus_tag="PWY98_000159"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2035949.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            57732..58906
                     /locus_tag="PWY98_000160"
                     /pseudo
     CDS             57732..58906
                     /locus_tag="PWY98_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            58910..59551
                     /locus_tag="PWY98_000161"
     CDS             58910..59551
                     /locus_tag="PWY98_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2035950.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            59548..60465
                     /locus_tag="PWY98_000162"
     CDS             59548..60465
                     /locus_tag="PWY98_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2035951.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            60462..61133
                     /locus_tag="PWY98_000163"
     CDS             60462..61133
                     /locus_tag="PWY98_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2035952.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(61674..62162)
                     /locus_tag="PWY98_000164"
     CDS             complement(61674..62162)
                     /locus_tag="PWY98_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2035953.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(62363..62629)
                     /locus_tag="PWY98_000165"
     CDS             complement(62363..62629)
                     /locus_tag="PWY98_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2035954.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(62629..62892)
                     /locus_tag="PWY98_000166"
     CDS             complement(62629..62892)
                     /locus_tag="PWY98_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2035955.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            63368..63553
                     /locus_tag="PWY98_000167"
     CDS             63368..63553
                     /locus_tag="PWY98_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2035956.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(63695..65518)
                     /locus_tag="PWY98_000168"
     CDS             complement(63695..65518)
                     /locus_tag="PWY98_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2035957.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(66116..68821)
                     /locus_tag="PWY98_000169"
     CDS             complement(66116..68821)
                     /locus_tag="PWY98_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2035958.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(69127..70074)
                     /locus_tag="PWY98_000170"
     CDS             complement(69127..70074)
                     /locus_tag="PWY98_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2035959.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            70264..70533
                     /gene="rpsN"
                     /locus_tag="PWY98_000171"
     CDS             70264..70533
                     /gene="rpsN"
                     /locus_tag="PWY98_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2035960.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(70709..71071)
                     /locus_tag="PWY98_000172"
     CDS             complement(70709..71071)
                     /locus_tag="PWY98_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2035961.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(71082..72203)
                     /locus_tag="PWY98_000173"
     CDS             complement(71082..72203)
                     /locus_tag="PWY98_000173"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2035962.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(72219..72569)
                     /locus_tag="PWY98_000174"
     CDS             complement(72219..72569)
                     /locus_tag="PWY98_000174"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2035963.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(72699..74210)
                     /locus_tag="PWY98_000175"
     CDS             complement(72699..74210)
                     /locus_tag="PWY98_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2035964.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(74532..75884)
                     /locus_tag="PWY98_000176"
     CDS             complement(74532..75884)
                     /locus_tag="PWY98_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2035965.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(76037..77113)
                     /locus_tag="PWY98_000177"
     CDS             complement(76037..77113)
                     /locus_tag="PWY98_000177"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2035966.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            77309..79054
                     /locus_tag="PWY98_000178"
     CDS             77309..79054
                     /locus_tag="PWY98_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2035967.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(79334..79771)
                     /locus_tag="PWY98_000179"
     CDS             complement(79334..79771)
                     /locus_tag="PWY98_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2035968.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(79755..81128)
                     /locus_tag="PWY98_000180"
     CDS             complement(79755..81128)
                     /locus_tag="PWY98_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035969.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(81450..81938)
                     /locus_tag="PWY98_000181"
     CDS             complement(81450..81938)
                     /locus_tag="PWY98_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2035970.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(81960..82562)
                     /locus_tag="PWY98_000182"
     CDS             complement(81960..82562)
                     /locus_tag="PWY98_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2035971.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        23850 a        17558 c        14999 g        26196 t
ORIGIN      
        1 gtatgaaaaa atatttcaat tttatttttt cgtttcaaat tattctatta gtgacatcct
       61 tgaactataa aaataggagt acggcatcat ctgccgcact cctatttttt tcgtcatttg
      121 tttcttaaat aaaacgattc tcacgcaatt ttattttttt gatatcttct actgtttgtg
      181 ttccagctaa ttgcatgacc atttccaatt ctgttttaaa gaaatcaaac acttgatgga
      241 ctcctgtact tcctcctaaa gacaatccat aaataacggg tctgccaata gcaacaagat
      301 ctgcacctga tgcgattgct ttgaaaacat gctgtccgcg tcgtacaccg ctgtcaaaca
      361 cgattggtac acgtttatct actgcttctg ccacatattg caacgaatca aacgctgctg
      421 gaccaccatc tagttgacgg ccaccatgat tagataccca gattccgcca gcaccagatt
      481 ctaatgaacg atagacatct tcttctgact ggacaccttt cacatagact ggcaaatcag
      541 aatgagcagc aataaattct acatcttttg gactaagctt ctgtttggaa gatttgtaca
      601 cagcatccat cgtttggcct actcctgatt gataagcctg aacgatcggc attggcagtg
      661 ggaaggtaaa cccatttctg cggtctgttt cacggtttcc gccaactgtt gcatccgctg
      721 tcaatacgat tgcttttgct ccattacgct tggccacttc aagaatatcc aaattgatcc
      781 catcatcttt gctcatataa aattgaaacc attgcggtgc tttctccccg cctgcttctc
      841 ttatctcttc taatgtacaa gaagcgtaag aacttgctgt atagatcgtc ccaaaatctg
      901 ctacaccttt tgcagaagct ttctccgcct tcacatgagc taagccatga gcagcaactg
      961 gtgccatgat gattggtgca gtcagcgttt ctttgtcaaa atgtgtagtt gtatcgggta
     1021 attctacatc tcttaataca tgaggaataa tcagctgatg attgaatgcc cgttcatttt
     1081 cttgataagt aaataagtcg ccagcaccac tgctgatgta accataacca ccagctggaa
     1141 tcacttgtga agcggctatt tctaaatctt ccatattgat aaaatccact attccttcat
     1201 ttgtacctgc ttgatacgtt ttttccattt caatgccttc cctctataaa aaattgaatg
     1261 aataatatga tagcgtttac acactttata ttactcttcc gtattgatac aggcaaataa
     1321 tctgtttaca tttcagtcat tccagttgtt actactctga aagaaatgtt tagaacacaa
     1381 atagataaaa aacgatggga ttttgacaaa agtcgtgtca aagtcccttt tacgaaatac
     1441 tgtggatgcc tattgaaata agtgcgggtt gtttctttgt atttcaatcg tttcttgcca
     1501 ttcttcgttt aattttggat taccgttttc ttccttgaac gcataaggtc ccaatccaaa
     1561 aagctcagtt agtatttgat tcacgattcc atatagctcg gtgtgatcgg acacatgatt
     1621 attgaagatc atctcattat caccacgttt gaatctcaca ttcgggctct catgatgaac
     1681 ctctatgcag ttttcccctt tttttaactg acggatttct gaaaacatct cataaatatc
     1741 ttctccaata aaaccttcag caagcgccag actgtccaca agaccgccta ctgtcaactc
     1801 agagattatc gctttgttgg caccaagctt taatgtatag cttgttgctt ctactacgcc
     1861 gcatgcattg caaccacctt ctgatagttc gctttttctg atcaattgaa cttctttcat
     1921 tttctgttcc tcctcttagt tttctactta tgtatttttt atcacctgac gtaagtgtat
     1981 tttatcgtca cgatcacgtg atttattctt cagagattct acctgaataa ttcatacttt
     2041 taattaaaac catgtgaaac tgcctcacgg cagcttacaa ttactttttc atcttcaccc
     2101 gttaagtgat gaaaaaagaa cccctttctg ctatggttaa agtgactaaa ccaaccaaaa
     2161 gaaaggagtt cttttcatga ctagcttaca caaaaaccaa gtaaaattca attcaaatat
     2221 cactatttct catacaggtg gtcaattatc gagtgattcg ggtctcgtcc tagtgaaaga
     2281 gctgatgaac atctttggtt tttctgaact ggctaagcaa cacattcaca ttgaagacga
     2341 acgagcgtat tttactcatg acaacttatc catacttgag cagttcatta tgcaattaat
     2401 tgctggttac tcagctgatt ctgcagctaa tctcctgaga caagatcctg tgtttaaagc
     2461 tgttttagat aggaaagaac tcgcttctca atcttcactt tcccgatttt tagatcgact
     2521 atctgaggag aatatccatg aacttcaagc tttgaaccaa gaacttatcg ataaagcacg
     2581 cctcatccgt aatgatacgg aattaatcat tgatttagat tcgacccatt cagatacatt
     2641 tggtcaccaa gaacaaacga attataatac acactaccaa acctatggtt accatccatt
     2701 ggtagctttt gacggattga ctggtgactt cttaaaagct gaactacgtt caggaaatca
     2761 atatacatca aaaggcgtaa aggagtttct cacaccttta ttagagcact ataatcactc
     2821 tctaccaaac actgacatct tggttcgtgg agacagcggg ttcgctacac ctggtgtgta
     2881 tgattcgtgt gaatcaaaaa agagtcatta tgttattcga ctgaagaata atcgtagact
     2941 aggtcaaata gctgagaaat cagtacttta tggcgataat caaaagtggg aagaacgaga
     3001 agttcagtac ttctcgacca cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg
     3061 tatacgctca acacgtgaag cgggcgaact actctttcga catgaattta tcgtgacgaa
     3121 tctatcagaa aatgtttctc ctgataccat cttttctatc tatgccaaac gtggcacaat
     3181 ggagaacttc attaaagaag cgaaagctgg cttttacttt gacaaaacag acagtcctcg
     3241 ctttttggag aatcatgtcc gaatgatgct cagcttgata gcttacaact tagtcaactt
     3301 cttaagaacg attggctttg aagaagtcca aaagggaatg accattcatt ctattcgatt
     3361 gaagtttctg aaagttgctg ggaaattagt ccaaacgggt agacgagtct atctcaaatt
     3421 atctagttat catgtgtatc agaatgaatt ctacagggtc tttgctcgcc tgaggcgagc
     3481 cagtcaatgg atctaatcca acaatctaac gattttttta ctgggaagtt atccagggag
     3541 aagtatgctc aaaatgcctt acacccaaaa tttattcctc aatatttttg aacagtgaat
     3601 gtttgaagta actttttagt caaaatcagt tgatagggat attagtgata catatttcaa
     3661 taaaatgtgc caaagaatta aagctatgaa ttattcaggg atcaattaga aagcaacttt
     3721 tttcgggatc taaatgaaat tctctcttat gatccagccc atagctatga ggaactatac
     3781 actcgcttga ttgactatgt ttacgcaaat acttttttat atcgcttatt tatcctcaac
     3841 catggtgata tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa ctatctgaac
     3901 atatggctat atgaagacaa gaaaacgatt atcacagaag aaatgagatt ctttactacg
     3961 taccacgtcc aaggttgtct atccgttctc aatcggtggg tagaaagcaa ttacaagtac
     4021 tccaaaaaag aattattaga actcttacag aaactgaatg atcacataga gaaaatcatg
     4081 ccttgaataa aaacatacta agcttagtta agaagaaccg taaccttctc gtataacaac
     4141 tgaatcttat ttagccaaat tgtttactga agattttttg agtaaaattt ctataattcc
     4201 tacttattga aaagtagttt gtagtgctgc ttctccctct ctaagtgata tatcttgttg
     4261 taccaatcaa attcacctca tcattaataa attactttta ggctaattta ttacaactta
     4321 taggttgaag ctgtaaccaa tttatagtca tattatattt ttatcatcca gtttattgaa
     4381 ttcttctcgt ttacctcaat tcattttcaa ggggaatcag ctcatcatct aacattttac
     4441 gccatgctcg cttcatttct tgtgggtatt taaatggttt cgcttctgga tgtttggctt
     4501 tatatccttc gtaatcctta ctgttgataa ttgctttcat attaggaatt ggatcttcac
     4561 ctggcatata ttgcagatta gaagcatgta attttttctc agaaacaaac cactcattgg
     4621 aaaagacatg aatcaaatca tcacttactt gtgtaaaata gcgacgttta atgacaaaga
     4681 tatcttcatt gctatctagt gtaccttcat ttctttgtgc ttaaattcaa agataatttt
     4741 ctcaataaag atttttgatt ccagtaagat tcctttactc cttttacaac atagcgttgt
     4801 cctttgtttt gaaacatctt cttcccctct ttgccattaa aaatagccca attctctata
     4861 aagaagatta agcttcatta ttataatata aagtttaaat ttggtttatt acttactttt
     4921 tcttattctc tagtaagcta atattgaaaa aaatgtaatc agatcattat ttttatatgt
     4981 tgtaacttcc tgataagaaa actaattaaa agaaagaggt aactacataa actaattaaa
     5041 aaagctaact tcatagttgg aggataaatg gaagaaactt caatgattgt ttctccttgt
     5101 gttccttcaa tcgacactag tcctattatc aatctctctt ttttgtccat ttacttttgc
     5161 gactgttccc caataatcaa atacaggaag cgttattttt tgtcctttag attcatgtac
     5221 tgtatagact attttcgaat ccatcacttg ataaggcata gtatgccatt ccttgccaat
     5281 tcttacttca tgattctgca aatgttcttt gtattgtaat gcttccgtgg gtgcatagtc
     5341 ataaagtcct ccgggaagga gtgtcgtgta acgaataaga ttttcttgat tcattaccaa
     5401 gtgatctctc tgagtgacaa ctttatgttc atagtttgct tttgttgcag cagatacagc
     5461 caacagtaat aaaacaattg ccgttatatt aactactttt ctttgcttag ctgatgtgct
     5521 cttcatgaaa acgaacgata aaatcaacga gccaaacagt acttgaaagc ctaagattcg
     5581 ccaaggaaat tgaatcattt gaatgggtgt attttgtaat aaagaccatg gaaaaaattt
     5641 ggtggataag agccaagaga ctaccgcact aaggtaaatc actttttctt tgcgattaaa
     5701 tcctttaaaa aagaagagtg gacagatcaa agaaataata ccaatgattc ctaccgtaaa
     5761 ggttgtcagg tcattattca tcgcaccaat caaactatcc gagacattta atgcttggtg
     5821 ttgtaaggtc gttttatctg gctcattaat cgattgaacc atgatttgtc ggaacatcgg
     5881 ataccaaaag tatgctgtta aaagaatcgt cattcctact gcctttgaca actccacaat
     5941 caaccggatg ctttcttttt ttcttgtttc acttactaca aagccgccta agactagtaa
     6001 gccaacggct attacactca taaaagtgga aagaacatgt gtataaatga gtaagctcat
     6061 actgactgct aatggaattg cacatggctt tttctcataa atcagtgcgt aagcataata
     6121 cataactatc ggtataaccg taagtgcaat cgcttcagca atagcagaac gatgataaat
     6181 atcaatcgta cggtacccat aaaatgtata taaacagcta aatataaaag ccgcatcgat
     6241 tctttttaaa aattttagtc cataatgaaa catcagcatg atagtacaaa cattcaatag
     6301 ccaaacatat aagatataac taacgattaa attattactt atccaataaa aaatgactgc
     6361 cggaaaaaag gtcaagtagg gataaaaata attcactccg ctcccataat gattaaacgt
     6421 cgtaaaattg attggaccag aaaaaattga agataaccct ttgactctag caaaatggaa
     6481 ggcaatatcc cccgatgtag aaatagtatg aacatttcta aaataattga ttgaataaag
     6541 tccagcaata attgctagga gaatggacaa tattattttt ttctttactt gcattactta
     6601 attatatact cctttcatgg attgctttat ctagtataac ttaaatttta aataaatcgt
     6661 tatttatttt tactctcact taaaaaagca ctaaaattat aagagaggga ggtaatggtc
     6721 tgaacaccaa gaattgttcc aacatcgaat tgcttaacta atgagttcaa agcttaatta
     6781 tgtggctctt atatttaccg agcgtggatt gcgatctctt tagcgaaaca aacatctttc
     6841 ataaaaaaaa cagccatatc cgctttcttg gatatgacta tttactacta ctttactatc
     6901 tttcattctt cttgtctcac ttatgataag gcgcccccgc attgatccgg aaggcacggt
     6961 agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt tttccaaaag
     7021 agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag ccgccgataa
     7081 caaaaacaag gtggcttttt ccttggatac ctaattggtc gatttgttta gcaaaagatt
     7141 cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt tctttgattt
     7201 tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct tcgcttaaat
     7261 tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag gcattcagcc
     7321 gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt cctactgaga
     7381 taattttgat attcatgttt tctctccttt ggtcttcatg ctcactttgt ttattataat
     7441 caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt ccccaacagt
     7501 tatgcacaac atccacaggt ttatccacag aaaacagtgt tattttacct tatttcatca
     7561 cattcattag gcaaacatac gttttataag aatttatcca ctttatacac aggctgtgga
     7621 tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt atctgtggaa
     7681 aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt ttcataaaaa
     7741 gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca gtagataaat
     7801 gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag atgaaaaaaa
     7861 gtaaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg atcgttggag
     7921 gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat agttctactg
     7981 ctacaagcgg caatcagaat tctgccggtg aaacagttgt ggaaaatgtc aaagtcaatg
     8041 tagattctga tatcaccaaa gcggtagata aagttcaaga cgctgtcgtt tctgttatca
     8101 acttacaaag tcaaaaccaa tcaagcggtt ttggcggact attcgggcag caagaagaaa
     8161 gtagcagctc agatgatagt aatttggaag cttatagtga aggaagcggc gtgatctaca
     8221 aaaaagacgg gaatactgct tatgtagtca cgaataatca cgtagtagat ggccagcaag
     8281 gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc tgaattagtt ggaacagatg
     8341 cttattcaga tttagcggtg ttgaagatca attcagacaa agtcgaaaca gttgcctctt
     8401 ttggtgactc aagtgctctg aaggttggtg agcctgcgat tgcgattggt tccccactag
     8461 gatctgaata tgcaaattct gtcacttcag gaatcatttc ttccttgaac cgacaagtga
     8521 caagtacgaa tgaatcgaac gaaacagtca atatcaatgc gatccaaaca gatgcagcaa
     8581 tcaaccctgg aaattctggt ggtcctttag ttaatatcga aggacaagtt atcggtatca
     8641 actcaagtaa gatcgcaagt acttctgaat ctacttctaa tgtaagcgta gaaggaatgg
     8701 gatttgctat tccaagtaac gatgtagtga atatcatcaa tcaactggaa aaagacggaa
     8761 aagtgacacg tccagctcta ggtatcacaa tggtcgactt atctgccgtt tctactcagc
     8821 aacaagaaca aatcttgaag attccttcat ctgtaactaa cggtgtcatc gtgacaaatg
     8881 tccaaacagc tacaccggct gaaaaagctg gattgaaaca atacgacgtc attactaaga
     8941 tcgatgacaa agaagtttct tctggtgtag aacttcaatc tgctttgtat aagaaaaaag
     9001 tgggagattc tgtcaaaatc acttactatg aaggctcgaa agaaaaaaca actactgttg
     9061 aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa tagtgaaaat taataaaaaa
     9121 gaggttgtga caaagacttg tcacaacctc tttattttgg aaatgcttct ccgactacgg
     9181 catcctcagg caatactaag attccttttt ttgctggagc atttggcaaa tgcaattcac
     9241 gagcggaaca gatcattcca tagctatcta cccctcgcaa agttcctggc cagatcatca
     9301 ttccatctgg catcatagct cctggttttg caacgacaac tttttgtcca gcttcgatat
     9361 tgggtgcacc gcaaacgatt tgcaatactg taccattgtc tacttctgtc tcagtaatcg
     9421 ataaatggtc agaatcagga tgtgggacgc atgtttttac gtacccgaca acgatcttcg
     9481 gttcatgatc agcttcaagc tgttcttcga atcctgcttc tttgatcaat cgattcaatt
     9541 tcgctaattc ctcatctgtt aagcttactt ggccgttccc ttcgattgtt agatgatcgg
     9601 agatatggaa aaagttccaa gcaacaactt gaccttgttc atctttgatc cgagcaacat
     9661 tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc ggctataata accatcaagg
     9721 tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt ctttcctcca ttatcctttg
     9781 ctgagattta tcttaacaaa aaattgcatt cactgcatga aatttttttc atgctttatt
     9841 tccgaagaag cctcttctgg aacaccattt attcgtaaca agggactgtg acaagacttt
     9901 tgtcacagtc cctttactgt tttagttaat tgattaaaaa ggtcgtacaa gttccacttg
     9961 cttaattact tggctgttct tcttcgtcag aaacgatcaa gacatcacaa ggtgaatgac
    10021 gtatcacgta gctggctaca cttcctgtca taaagcgttc gactgcattt agacctgact
    10081 gcccaaccat gattagatcg atctcatatt tttttggcaa tttgactgcc atggattcac
    10141 gaacagaacc atatgcaata attccttcta tatttgtgta atcaacagat ttcccatatt
    10201 ctttacattc ttcgatcatt tccttggctg tttcagtttg ctgatcgact agattttgat
    10261 caataggtgc atagcccata aagttataaa attgctgatc gattacgttt gctacatata
    10321 ctttcccatt attacggcga gccacttcta ccgctttttt gaaagcagca agtgcttgtt
    10381 cactaccgtc gatacctact aaaatattct tatattcttg attcatcttg acccctccta
    10441 acagatgtca gccttttgtt acactttcta ggaacgattc gatttcctgt tttgttttac
    10501 gatctttatt cactagacga cctaattctt ttccgtcttg gatcacgaca aaacttggga
    10561 taccaaaaat gttccattca gcggctacat ctatgtactt gtcacggtct acttcaataa
    10621 attgccaatc agaaaagtca ttttcaattt caggcatttg cggtttgata aaacggcagt
    10681 ctccgcacca gccggcagta aagaaaaata cactctttcc agtttctacg tagcctgcta
    10741 attcttctaa actatttggg ataatcattc tttgtcctct actttctgtt tatgtatttt
    10801 tcaattaaat tatacacctt tctttcgaac taacaaacat tgatcctctt tttataaaaa
    10861 aaatctggca aaaagcaatc ttttgccaga ttttatagtt tcactatcgc attattttcc
    10921 gtatacgatc gtatttactg ttgatttatc caaacctttc agtgtttgaa tcaacatttc
    10981 tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga tgcgtatgga tataacgtcc
    11041 cactacgcca atcactgtac ttgggattcc ttcgttttgt gtatgagcag cacctgcatc
    11101 tgtaccgcct tttgaaacaa agtattgata agggatattg tttgtttcag cgatatccag
    11161 caagtactcg cgcaaacgag gaagcatgat caaacctgga tcttggatac gcatcaatgt
    11221 accttctcct aaatgtccga atgtgccatt tttcgtcact gtatcatctg ctgcagagca
    11281 gtctactgca aagaacagat ctggcttgaa tttattgaca gaagctttag aaccacgcaa
    11341 gcctacttct tcctggacat tcgcgcctgc aatcaatgta tgacctaatg tttcattttg
    11401 caacgcttct aaagcttcta ataccattgt acagccatag cggttgtccc atgatttact
    11461 gataatattc tttccgttgg ctgttttgat cgtttcagcg taaggaacga ttgtgtctcc
    11521 tggaaggaca ccgtaactca ttgcctcttc tcttgattca aacccagcat cgaacaaaat
    11581 atcggttact tctactgatt tttgtccgct tgttcctcgc aataaatgtg ggggaacgga
    11641 agaagaaata catggataat tcttaccagt agaggtttta agtgtaaaac gctgagcaga
    11701 aacaacgtaa ggattccagc cgcctagagg tacgacttta aaaagcccgt tttcttggat
    11761 ttgtgtcagc atgaatccga cttcatccat atgtgctgcg accattacac ggggtgcatc
    11821 cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa ccatctaact gtaactcatc
    11881 taccagcggt tccatatgac tttccatata agcgcgaaca tcttgttcaa aaccgcttgt
    11941 tccttgtaat tctgttaact ctttgattcg ttgaaatgtt ttttcttcca tatgctattc
    12001 gtctaaattt cagactaaac tcctctcaga cttatattga tctgcggata aaatatgatt
    12061 ttttctgcag accgatcgta tttattatag cttatttttt ctggtagcgg ttatttattt
    12121 caaagaaaat atgagagtgt ggtaaaataa gcacaaggag taacaagtga gaagaaggag
    12181 gtatgtccac atgtatgaag aaagcgaact aagctatttt aaaggcgggt tagcagttgg
    12241 aatcggtcta ggattattga gcggggtagc ttcaacgatt tggtatcatc gcaaaaaagt
    12301 attgtcagca gaccaagtac ttgaaaatat caagtcatct tttctaaaag aaggtgcaat
    12361 cgaaggttct tggatctctt ttgaaaagga acctattagg aaattcgccg ttcggtcgag
    12421 tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa actgtttatt atgaattttt
    12481 agcagatgct catacaggaa ccgtattaga aatttcaaga aaaaaaggtg caacagatta
    12541 atacgtcaaa aaagtgagag aacgaatacg cacccactat tcaaaagaga ggtatcatgt
    12601 ccttcttttg tagtgggtgg ttttttagat agagctttcc aaacatatat tccagtattt
    12661 tctttttaaa taaatgatat ttagcgggaa aaactagcat ttcctttgat tatgaggtat
    12721 ttttaattta gcttttgttt tctatagtta aggagagtga aaaatggaac aacaagaaaa
    12781 aattgctgtt ctgattcctt gttacaatga ggaagcaaca atttcaacag tcattgcaga
    12841 ctttaaacga gaattaccag aagcagatat ttatgtctat gataataatt cgaccgatcg
    12901 aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa aaagaacctc gtcaaggaaa
    12961 aggaaatgtg atccgccaaa tgttctttga tatcgatgcc gactattatt taatggtaga
    13021 tggcgatgat acttatcctg cagaagctgt acatggtttg ctcgagaaat tacgttctgg
    13081 tgaagcagat atggtcattg gtgatcgcct atcaaatgga acctactttg atgagaataa
    13141 gagacctttc cacgattttg gaaataacct tgttagaaat acaattacta gaatgtataa
    13201 aacaaaaatt cttgatgtca tgaccggcta tcgaggattc aatcgtatct ttgtcaaaag
    13261 ttttcctatt atgagttctg gttttcaaat cgaaacggaa ttgacgatcc atgctttaga
    13321 taaaaaattc aagttcgttg aaatcccaat cgattatcgt gatcgtcctg aaggcagcga
    13381 atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatta ttaaaatgtt
    13441 taaagattat aagcctttgc ttttctttgg gatttggacc atcttcttct ttttatttgg
    13501 attggtcacg gggattcctg tgattcgcga atatatgctg acaagcttta ttacacgcat
    13561 cccttctgcg attttaagta ctggattgat gactttagca cttctttcat tagtaacagg
    13621 gttgattttg gatacagtcg tcacgaacgc taagaaagaa tatgaattga atctatatca
    13681 tgtttatcat gaataccaca gtagaaagta aaaaaaggat caagactgca aaaataattt
    13741 gcaattcttg atcctctttt taattaatta tttcgccaaa cgatagatgg catctgcata
    13801 gattgctgtt gctctgaaca agtcatcaag actcataaat tcgtttgctt gatgcattgt
    13861 atcagtatat cctgggaaca tcgctccata agcaacgcca cgtttcaaca aacgtccgta
    13921 agttccgcca ccgatgattt gttcgtagcc tttttctcca gtgtggtctt catagacttg
    13981 caataatgta gccaccaatg gatcatccat tggtacgtaa tgtggttcca ttacgcgagc
    14041 acctcgagta actgttgcgc cttcttgccc cactgtttgt tcgatgtcac tttgcaatcc
    14101 gtctactgtt acaccttttg gaaaacggaa attcatattg atgaagttcg cttcctctgt
    14161 accattttct tcaaaagcaa atagtccagc attcattgtc aatttgccca ttttttcatc
    14221 ttcataagcg actcctagtt tttctccata aaaatcttca tggacataag cagctgctac
    14281 atggataaat tgtttagcag atcctaggaa ttcatagtta tctaagaaca atgccaagaa
    14341 agaacctgca ttgataccag attgtgggct tgcaccatgt gcgcctttac caactaattc
    14401 gatttttaca tacgtattgt ccgcttcaat cgttccgctt actggatttg cttcgatgaa
    14461 ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca cttggtactt gaagtgctgc
    14521 agttgctgtt cctggcacca tattttcgcg aagtccggaa acaaaagatt tcaagacata
    14581 atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa acatttcctt tttcaccatt
    14641 gatgattggg aattcagcat ctggtgagaa accaaaatct ggttcttctt catgttcgaa
    14701 ataataagcc atatcgcccc agccgctttc ttcatcgctg ccgactacga agcgaacttt
    14761 ttttgatagt ttcaagtcaa gttctttaat gatcttcatc gcatagtaag ctgccatact
    14821 tggacctttg tcatcgcttg atccgcgtgc aaagatcttt ccatccttga ttactggttc
    14881 ataaggatct gtgtcccagc catctcctgc aggaacgaca tccatgtgtc caaatatccc
    14941 tagcgtttcg tctccttcgc caagatcaat atgccctgca tagttatcga cattttttac
    15001 tttaaatcca tcacgttcgc cataagctaa catatgtttc aatgcgtcgc gaggtccagg
    15061 tccaaatggt gcatctggtg ttacttttga gtcatcgcgc tcgctgttga cacgtaaaag
    15121 atttttcaag tcttcaagca agtcttcttt acgtgcttct acttcttttt gccaatcaat
    15181 tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt tcatgatacc acaaattaca
    15241 gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta tctgtttttc tctaaaaaaa
    15301 atatacgcta taatgagtaa aaggaggttt ttagatgctg ataaacgaac aactagtgaa
    15361 aagagtcatc acacctcgtc caaaaatttc acataaagga acatttggac gtatcgtttt
    15421 gattggcggc aatgaacaat atggcggagc aatcattatg agtgctttag ctgccgtaca
    15481 cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa aatcggagtg ctcttcacag
    15541 tcatctgcca gaagcaatga ttgtagtttg gacggatact gaccagtttc tttccgtaat
    15601 aaagcaagca gatgtactct tgattggtcc cggattgggt acttcttcta agagtttgga
    15661 acgtttcaaa caagtactag aaaaccaatc aaaagaacaa tggctggtga ttgatggctc
    15721 tgctttaagt ttacttgctg ctaatcatct tttgctaccc tatcccgaac aaactgtact
    15781 tacacctcat caaatggagt ggcaacggtt gagttctcta ccgatacaag aacaacggga
    15841 taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg ctcgttttga aaagtcatga
    15901 gacaaaaatt tatggtcacc agcacttcta caccaatcct ttaggttctg ctgctatggc
    15961 aacgggcggc atgggtgaca ctttagctgg gatgatcaca ggatttttag cccagtttcc
    16021 tcaaaatgac caaacgattg cagcagctat ttatcttcat agtttcattg gagaccagct
    16081 ggcaaaagaa caatatattg ttttgccgac acaaatcagc gaagcacttc cgaaatggat
    16141 gaattatttt tgtaaaaaat caatggaaaa cgaggcatga gtcatggaga aatttacagg
    16201 ttatgtgcga gaaccttttt attatgaaac agatcaaatg gggattattc atcacagcaa
    16261 ttatatccgc tggtttgaag aggctcgggt agccttattg gtacatctag ggtttcccta
    16321 taaaaaaatc gaagaatctg ggatcatcat ccccgttctg gaagtaaatt gcagctataa
    16381 agaaatgatc cattacggag aaaaagtgag catccatcca actatccaaa agtatacggg
    16441 gacacggttg gattttagct acgaaattgt gggaactgaa gacaaaaaat taaaaactac
    16501 tggcagtagt aaacactgtt ttttactttc tgattcgcaa cgattagtga agctgagcaa
    16561 agtcaatccg gagttagatc agttattcca agcgtacgct tctttggatg atgtaaattc
    16621 ttaataaaga ccgtctaagc atttttcttg tattgtgtcg agtagttcgt ataatgaaaa
    16681 gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat gcacggttac atcgataaaa
    16741 ctggctgtcg cactcgggaa aaaaacttag acaatctgct cattttcaaa gacaaaaaag
    16801 aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa accgatccaa gaggtacgac
    16861 cagattgatt caatattttt cttttcgagg ctctaatagg catgcctatc agggcttttt
    16921 ttgcagactg tgtttttatg cccacacgat ttgtaagcga tttatgttat aatagattat
    16981 atctgcatag aaaggaaggt taaatggtta tcaataattt gcaacagcaa gcttatgaat
    17041 ctatacgtaa acaaatcatt tattccgagt tagcacctgg aagtaaaatc tccgataaag
    17101 tgttagaaga acaactagct atcgggcgta caccgattca agaagcactg atccaactgc
    17161 gcaatcaaga gcttgtaaaa accattccac agtccggcac ttatgtttca aaaatcgata
    17221 tccgatctgc ttcattagcc cgttatacgc gggaaaagtt ggaaaaaccg atcctgcaag
    17281 aatgcagtgc gaaaatgacc aagcaagatc aagctaaact agaaaaaata ctagaacaaa
    17341 ccgatcaggc agtaatcgct caaaataaaa aagctttttt tgatttggat actgattttc
    17401 accgaacttg ttatgaaatt gctggaaaac gagaaatttg ggattggcta gaaagttata
    17461 gtacgcactt gaaccgtttc agatggttac gtttgacgat ttcagaatta gactggggtc
    17521 gagtattaga tgaacatcag acaatgcttc aatcaatgat tgatcataac tttgatgaag
    17581 tcggatttct ttgcacgatg catttacata tgattatcga ggagcaagaa tacgtgattc
    17641 ataattaccc cgattatttt gaagatattc aggacagacc catcaaataa gaggttttgg
    17701 aatcagcgtt ttgacctgag tattagagaa agaaacggca aaatagttcc tcatattttg
    17761 ccgttttttt tgagctaata ccgagggtca gctgattgca caccgtttat tgagaggttg
    17821 tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaatagc ttcttatgtt
    17881 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt ttccaagagg
    17941 tcgtgagcct gccgttcatc tccgagtcac aaggaacaat ttcaaaaaac agcttctcat
    18001 gtttgttgaa tattttgact tgtgaccgag gagttgacag gagaacatcg tttattcgga
    18061 aaaaaaggac tgtgacacga cttttgtcac agtctcttta tttgtaatct aaatcatact
    18121 ccgtacatct tttttctatt ttttttattt cttctaagat cccttggcta cgaatttcat
    18181 tatatggcca aagtaagact gcctctaata cataaggcga aaaatcgctt tgattcatct
    18241 cttttgaaaa actcatcgct gctatctcgc tcaatgcacg aatgtttgat tgatgtgtat
    18301 aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt ataaattccc ttaacagaag
    18361 cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc ttgcgctgtt tctttagaga
    18421 tcgaagctag tcctgaacat tttaattttt ctaacggctc cgtcatcaat cgaatccctt
    18481 catcttctgc ataagttgca aaagtgattc gttcgtttat ttgctctgat tcacaggaaa
    18541 cgattggaac agccatttgt tcacaaattt ctcctacatc ggcaaaaggg tacttttttt
    18601 tgatgctaca tgccgtttcg ataccacaat cgattgattg ttgtataaga attttctgct
    18661 tggacaaagg aatctttttc caaaataaat ctcgccgaaa ctggtagaat ccccaaattt
    18721 cttcggggat ctgttgtaat tcgtttagta actcttctaa cttatgtttg tttgatattt
    18781 ccggcaacga cgatcacttc ttcctccggt tccatagtag atacttccag atccatcaaa
    18841 agctgtagtt gttccagatc cgtcgctgta aaatcagaga ctcttgctcc taagcagaga
    18901 taaaattctg gtcgggcaat caattcagtt tgataaactg ccatatcttc ccaaagttgt
    18961 tcgacagctg tcatataatt ggctgcagtt gttttcatac atgaagcatg tccacgttgg
    19021 accttgataa atcctttttg atcgatgatc cgaagacttc cagcattttt tgaacgattc
    19081 tgttcgccgc atacataaaa gaaaggtgta ttttccaata agcaaacttc atttttattt
    19141 aatcgcatat cctctgcagc aagctctagt gcttcttctg ttgtgatctc cttggttaga
    19201 tcggttgctt tcacttccgt agaaccagta gcgatagccg ttacttttga agtctgtgga
    19261 tcgatttcta cgtggacttc gatcgattct ggtgtagcgc ctgattcaat cgctttattc
    19321 atcgcttcat ttttcaacga tcgtatatct tctttacttg gcgaagggat gatacgttct
    19381 acaacatctc ggaccattgc aagtgctaca ccaatagaag aaatcacttc ggcgttttca
    19441 ggaatcgaat atttcacacc cattttatta ctaaagtaag taatcaatga ggcagcgccg
    19501 ccccctacac ctacaagaga aatctgatct ttctccaagt gatacttgtc tgccaattct
    19561 aaaatcaccg gttcaatctt agcataggat ttttccatga tctgttctgc gatgtcttca
    19621 accgtcgtat gacaataatc agctaatgct tggatggctt ttcgtgcaga tgggacattt
    19681 ccataagaaa aatgttcttc ttgcactaat ccaaggacat tggctgcaca tgtatttgta
    19741 atagtcactt cttctccgtc ttccatcact accttgacgt aatcagcagg gtctccttct
    19801 ttaggagaga aaaattcaac cttaggacct ttgatttttt ctgtctccgt aaatactgca
    19861 taatctagac cggcaatatg tgcagaacgt ggaccgacat cgatgattcc tgactgatta
    19921 gcccgtacca tcgaaccgcc tgccacacct agaacccgaa catctaatga agagatatat
    19981 gttgggtggc cacctacaat tgaataatcg attgctggac gtccattttt aatgaccccg
    20041 atattggttg ttgttccgcc tacttcaaaa taaacaccat tagacgctct caagtacatc
    20101 aaagagccca tgactgatgc tgctggaccg gaaagcatgg ttaaaacagg tcgttttttc
    20161 atttcattga tttccattac tccgccgtct ccgcgcataa tcatcaaaga aacattgacc
    20221 cctgcttctc gtacggaatc ttctgtagag gttgccgtat ctagcatttt tggtaaaata
    20281 gatgcattga ttgctgctgt ccgagttctt cttgtcaatc catataattt tgtgatgtca
    20341 gaagccatcg ttgtagggat cgattttacc gatgctgctt cataaacaac tcgttctggt
    20401 tccccattat ctactccaaa tgccatagaa gaaaccaaga cctccgctct ttctctttct
    20461 aaagaagaaa tcgtttctga aacacggtct acatttaagt gtttgacagg taagaatgca
    20521 ttgacaatct ctattttttt cttattccct aaatcgatat cgttcaatct agtttgtcgt
    20581 tttgctaaaa aaccttctaa tccacctttt gccatcccga ttacgccgac ttttgctacg
    20641 tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat gtgcaacaaa cacaacatct
    20701 tctggactga tattattttc cagcaaacaa ttttgaaaag actgaacgac acctgcggct
    20761 actccacgga catcatcgtg ggttgttttc actgaagatt ttccgatgat ttcgtgcgtc
    20821 gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa catcaattcc cattcgcaca
    20881 aaacgtttca ttttcttttc ctccattcat ctaatcgttt ttatcgacgt tttgtttact
    20941 aggcgttccc gtacaataag aacgtgatca ggcataagat cgctccgatg atccaaccag
    21001 ttgggatgga tttcttcata tattctcgag aagttacttt tgtataacct aatccccaag
    21061 caacccaaga ttgtgtaata tctaaatgct gaggagcaat cgttgtgatt gcaaataacg
    21121 ggaacaaaaa ggctggcgac atcgttggat taacagctaa tacgacagct aaaatagctg
    21181 agcctgaacc gactagattc attggaccgc ggaaaaatcc tagtggtgtc aatatagcaa
    21241 atacgattgc caagactaat ggtgattgcg gtatccaatc tccaaaaata gctgaaaaat
    21301 aaggagaagc atacgcagct gaattattga acatcgctaa ggtcatcaag aaaccaacca
    21361 ttggcgcaac atctactgcg ccatctgtaa ataatttcgc aaactgacga cagatttctt
    21421 tataacctcc gcgcaatttt ccagtaaaca gcaatgccca gatacctgct agaataaaac
    21481 ctaagatgat tggaagatct aaaagaacga cccctaaaac tggtaataat acggctaacc
    21541 atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt tgctgccatt gcatagcgtt
    21601 tctttttgtt cattgatata ttagcgaccg ttagtaccac aactaagctt acaatcatcc
    21661 caatcatacc aatttggaaa tagtcattat aagtatagct ttcagccgct ggattgaaac
    21721 cagcataaat cgtttgatat tgtttgaaat tgacgatatt agcaaagatt ccagccataa
    21781 tcgagcccat aaatgagaac agtgcaaccg gtactgaaat acctaatgac atcaagattg
    21841 gcaacacaat aactgcaata gaaatcactg gtccgattcc tgtcatcgac atgaatataa
    21901 cagcggttac aatacatagt aagctcatcg tgattcttgg cttgtcccct ccaagttcaa
    21961 ctacctttcg aatcagggta gctgcgattc cgctatctac tagaacacgc ccgaaaaagg
    22021 caccaaagaa gacatttacc aagatcgatt tggcgtactc agctggcccc gtcgtataga
    22081 tatgcgtcaa cacatcaatt actccttgcc cttccatcgc cgggtttggt gaaaacgtgt
    22141 ttccgactag cgctaatccc atccatacag tagccatgat ggcaaagccg accatcaagt
    22201 tgtagccttt tacacagtac cagaccatga aaaagaatga gataactaac aaaataccaa
    22261 taataatatc actcatgtta ttcctccgtc cattttgttt ttctgataag caaactaata
    22321 tatcagtcaa actcattcta acttatgtag ttagcgcttt cattaaaggg tgaccataaa
    22381 tgttcagttt tcaattaaaa aaccttaatt atgctatttt agacttatac gttattgtcc
    22441 tgtttcattt gatggaggat tgtaaataaa aaaagaggaa agaacataac ttttcgttat
    22501 cttctttcct ctttataatt gtttagctta tttagccaaa gcttcttttg cttggtcagc
    22561 caatgcagta aatgctgctg catcgttaac agccaagtca gctaacattt tacggttgat
    22621 gtcgatttca gcaactttca aaccgtgcat taattttgag tagctcaagc cattcatacg
    22681 agctgctgcg ttgatacgag caatccacaa tttacggaag tcgcgtttct tttgacgacg
    22741 atcgcgatat gcgtagttgt atgaattcat tacttgttct tttgctgttt taaataaagt
    22801 gtgttttgaa ccgtagtaac cttttgctaa ttttagcact cttttacgac gtttacgagt
    22861 tactgttcca cctttaacac gtgccatgta taattcctcc taataaaaat aaatcttcag
    22921 attctgttat ttgtacttct tatttcattt ttgctagttg ttggcgaata cgtttgaagt
    22981 cgccactaga aaccatgctt gctttacgca attgacggcg ttgttttttt gttttgccgt
    23041 ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc gcctttacca gtacgtttga
    23101 aacgttttgc tgatccgcgg tgtgtttttt gttttggcat gactatattt cctcctcaaa
    23161 atctttcgtt atccgactat cggctgtcca acagccagtt agtcattcaa attacttact
    23221 gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg tccattttcg ctttttgttc
    23281 cactgttgca atatccgcag tttcttcagc taagcgattc aagacttttt gaccaatttc
    23341 tttatgggta atggcacggc ctttgaaacg gatagaagct ttcactttat cgcccttttc
    23401 caagaactta cgtgcattac gaagttttgt attgaagtca ttcaagtcga tcgtaggact
    23461 taagcgaact tcttttacat tgatcacttt ttgtttttta cgcgcttcgc ggtctttctt
    23521 ttgttgttcg aaacggtatt taccgtagtc catgattcgc gcaactggtg gtttcgcacc
    23581 aggggcaact aacacaagat ccaagtttgc ttgttctgca atattcaatg cttctgcttt
    23641 tgtcttaact cctaattgct caccatctga accgatcaat cgtaactctc gtgcacgaat
    23701 gccgtcgtta accatcatat cctttgctat ggtcattcac ctccaaatat tatgagagaa
    23761 agaaaagctt accgaataaa cggcaataaa aaaacgagtt ctcttacaga acccgcgcgt
    23821 acttcaccta atagaaccag tgaaaactat ctagcccagt gacattatca actaaggcga
    23881 gaagcgggtg cttctgcttt tatttctcaa ccatcatagt atagcaaaaa ctaatctttt
    23941 tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa atgattagat tagcagactt
    24001 tcttgcagtg attcccgctt ctttgctata ctaaagaaaa acgaggtgaa cccacatgaa
    24061 aaaaatcgaa cctattccac cttctcttcc tcttcttaca aagggagagt tttatttaga
    24121 agatgaggag atcttctctg gtcttctgat caacgggggt gaccaaagct atctcgatgc
    24181 caaaaatatg gtattaagag agagtcatat ccaaaaactt acgatgcaaa aaacagtttt
    24241 acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt gatttttcca atctggagtg
    24301 gattggtggc agtttccacc aagtcgtgtt tcatcaatgt aaattaacag gtacgaattt
    24361 tgccgaaagt tatcttcgag actgtacttt cactgattgt attgcttcga tggcttcctt
    24421 tagtagtaca aacttgaaag ctgtctcctt cgatcattgt caattagaag acagcgaatt
    24481 ttatgaggtc acttggaaaa accttttttt gtctgacaat cagttgaccg gttcgaactg
    24541 gtttcgaact tctctgaagg gattagactt tacgactaat ttcttcacag caatcgcttt
    24601 atctcaagat tctttggcgg gactaatcgt caaccaagag caagcacttg tgatcgccca
    24661 agcccttggt ttgcagatca aagactagat caacgaatcc gccgccacag tgcaataagc
    24721 atccaaatag ataagaccaa actgatgatc aaggcgattc cccatccgaa aacggaatgg
    24781 gtaaagggta actggagatt cattccgaag aatcctgtca caatagttgg aacggtcaag
    24841 atcagcgacc agaccgtcaa aaatttcatc gtgtcattca agttgttatt cagcaaatta
    24901 ttataagtgc cagagagctg gtctaaaatc tgcgaagcta agttcgtcat ttcaactgct
    24961 tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt ccttttctga caacttgcga
    25021 taaatagcca atgccttgat ttgttctaaa agtactacat tttgttttgt tcctgtaact
    25081 aagtaaacca aaccgatctc taaatctgaa agttgcagca gattcttgtt cgttgttttt
    25141 tctcttaatt tcagattaag ccgctgacgt tcactattca cttcctcgat taaaggaaaa
    25201 aaggaatcag aaatcaaaaa caatgtacga aacaataaac tgtatacact catgtctggc
    25261 attcgttcca aaagcgatcg aattagtcga atcacgtact cggtattcgg cgtggtaaat
    25321 gtaaacaaac cgtcttcttt taaaataaaa gcgataggac tagtttcata atgattatcg
    25381 actttttgct ggtgcggaac atttgataca agcagtaacg cttcatcaaa agaatcgtac
    25441 tctacgcgcg cccgttcatt tttatctaga gaataagtca gcatctcgtc actaatgcca
    25501 tattcttctt ttaagttttt aacagctagc acatcgtttt gctgtacatt gatccaaaaa
    25561 ttacggtctt gattaaaata gtgtttttcc cgcatactct atcctcctga catttgtttc
    25621 cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc aactgtaaat
    25681 caaatggcta ctttggtttg gcgaatctct gctaattttt cttcgatcat tttcaagact
    25741 tcttcttttg cttgaggatc ttccacaaaa ttatagcggt ctccatcgat ttggattttt
    25801 ggactttctt tgtattcttc ataccattga tcgtaacgtc tgttcaattc tttgtaataa
    25861 tcgtatagac ttggatcaaa ggacaattgt tcatacgaac gaccacgttt ttcgatacgc
    25921 tccaacatcg tatcaaaaga tacacgaata tgaactaata aatccggatg ttttttatgg
    25981 gcagcatagg gaagttcttc catcatgttt tccagcagtt catcatacac acgaacttct
    26041 gtttctgttg ctcttcctag atctgcattt agatggaaaa gcagtgaatc ttcataaatc
    26101 gaacggtcca atacattatt gtcatctttc attgcttgtt tgatgcttgc aaaacgttta
    26161 ttcaaaaaat aaatttgcag caaaaaagca tactgttcag ggtttgcata aaataagggc
    26221 aagacttcat tatcatcaat cgattcataa aatgcctgac tatcaaagtg ttcagccatc
    26281 atttctgtta aacttgattt tccggcacca attgtacctg ctaaaacgat cacgctcatt
    26341 tacctccagc tcacttcatc actaaatatt gtgtttaact ccaacctttt taaatatacc
    26401 cccaatatct agtagtgtca attgtatttt tttctagtca aagaagaaaa aaagtcaaaa
    26461 aattcttaag aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt acttctgaga
    26521 tggttttagt aaaataaagg aggaatgttt tttgagaccc ttagtggaca aaaaaattcg
    26581 tgaagcagcg caactcgttt tgggcgcttt attttttgct taaaaatcac ttttacgtga
    26641 gaaggagtta gattatggca aactatggcc aaggagaatc aagcggaaag atcatattga
    26701 tgggcgagca cgcggttgtt tatggagaac cagcgattgc ctttcctttc tatgcaacaa
    26761 aagtcaccgc attccttgaa gagctggatg caatggacga tcaactggtt tcttcctact
    26821 attcaggaaa tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa ttattcattc
    26881 acttaaaaaa acagcatgac atccaaaaaa acttgcaact gaccattgaa agcacgattc
    26941 ctgctgaacg tggaatggga tcaagcgctg cagtcgccac agcagtcact cgtgcttttt
    27001 atgattactt agcatttcct ttgtctcgtg aaatactatt agaaaatgtc cagctttcgg
    27061 aaaaaatcgc ccatggtaat cctagtggaa tcgatgcagc cgctactagc agcttgcagc
    27121 cgatttattt tacaaaaggg catcctttcg actacttttc tttgaacatc gatgcttttt
    27181 tgattgtcgc tgatacagga atcaaaggac aaacaagaga agccgtcaaa gatgtcgctc
    27241 acctctttga aacccagcct catgaaactg gacaaatgat ccaaaaatta ggatacttga
    27301 cgaagcaagc aaaacaagca atcattgaaa attcaccaga aacgttggca cagacaatgg
    27361 atgaatcaca atcacttctg gaaaagctga caatcagcaa tgattttctt aatctattga
    27421 tccaaacagc aaaagatacc ggggcattgg gtgccaaatt aactggcggt ggacgcggtg
    27481 gctgtatgat tgcattagca caaacaaaaa caaaagccca agaaatcagt caagcacttg
    27541 aagatgcggg tgctgctgaa acttggatac aaggattagg agtacatacc tatgtttaaa
    27601 ggcaaagcac gcgcatatac caacattgct ctaatcaaat attggggtaa gaaaaatgaa
    27661 gaactcatcc ttccaatgaa caacagcctt tcgttaacat tggatgcatt ttatacagag
    27721 acagaagtca tcttttctga tagctatatg gtagatgaat tttatctaga tggcaccttg
    27781 caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg acctttttcg taaagaagcc
    27841 ggcctctcac taaaagcttc agtaatcagt cagaatttcg ttcctaccgc agctggatta
    27901 gcctcctctg ccagcgggct agctgcttta gcaggcgctt gcaatactgc tcttaagctt
    27961 ggattagatg atctctctct ttcaagattt gctcgacgcg ggtctggttc agcttgccga
    28021 agtattttcg gtggtttcgt cgaatgggaa aaaggccatg acgacttaag ttcttacgct
    28081 aagccagtcc cttccgattc tttcgaagac gatttagcaa tggttttcgt tttgatcaac
    28141 gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga aacatccagt
    28201 ttttatcaag gctggttaga ttccgttgaa ggggatctat atcaattgaa acaagcaatc
    28261 aaaacaaaag atttccaact tctcggagaa acgatggaaa gaaacggact aaaaatgcat
    28321 ggaacgactt tagctgccca gcctccattc acttactggt ctcctgacag cttaaaagcg
    28381 atggatgcgg tgcgacaatt gagaaagcaa ggcattccat gctactttac gatggatgct
    28441 ggccctaatg tcaaagtttt agtggaaaac agccatttat ctgaggtaca agaaacattt
    28501 accaaattat ttagcaaaga acaagtaatt actgcacatg ctggtccagg aattgcgatt
    28561 attgaataaa gggagatttt tatgattgaa gtatctgcac caggcaaact ctatattgcc
    28621 ggagaatatg cagttgttga aacaggccat ccagcagtta tcgctgcagt cgatcaattc
    28681 gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc acaatatagt
    28741 gggatgcctg tacgttggac aagacgcaac ggagaattag ttttagatat tcgggaaaat
    28801 ccttttcatt atatccttgc tgctattcgc ttgactgaaa agtatgcaca agaaaaaaac
    28861 attcttttat ccttttatga tctgaaagta acaagtgagt tagacagttc aaatggccgg
    28921 aaatatggat tgggttctag cggtgccgtg accgttgcaa cggtcaaagc cttgaatgtt
    28981 ttttacgcgt tgaatttatc tcagttggag attttcaaga ttgcagcact agccaattta
    29041 gcagttcaag ataatggttc ctgcggcgac atcgctgcta gctgttatgg tggctggatc
    29101 gctttctcaa ccttcgacca tccttggctc caagaacaag aaactcagca ttctatcagt
    29161 gagttacttg ccctggattg gccaggtcta tccattgagc cattgattgc tcctgaagat
    29221 ttacgtttat tgattggttg gacgggtagc cctgcctcta cttctgattt ggtcgatcaa
    29281 gttcaccgtt cgagagaaga taaaatggtg gcttatcagc ttttcttaaa aaatagtaca
    29341 gaatgtgtca acgaaatgat caaagggttt aaagaaaata atgtaacgtt gatccaacag
    29401 atgattcgaa aaaaccgaca attactgcat gatttatctg caatcactgg ggtcgtcatc
    29461 gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac agtatgaagg agccgcaaaa
    29521 tcttctggtg caggtggggg cgattgcgga atcgtaattg ttgaccagaa atctggcatt
    29581 cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt acatgtctat
    29641 agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca ctagctaaag
    29701 cctttcatga taaacaaaaa aacgaatttg acttcgtacg gatcatccat aaccctttgc
    29761 cgcaaatagc agtttctgat gtcgatctaa gtacacaggc agttgggttt acactaagca
    29821 gcccatttta tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa atcaaccaag
    29881 atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc cactggttca gtcagcgctg
    29941 ccttgaaagt cccttcttta gccgatactt atacgattat gcgacaagaa tatcctcacg
    30001 ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga aagagccaaa gaagctatcc
    30061 gattgtttca cgcagatgcc ttacagattc atttgaatgc acctcaagag ttggttatgc
    30121 ctgagggaga ccgtgatttt accaattgga aagtacttat tcaagaaact caaacagcca
    30181 ttgacgtccc cctcatcgta aaagaagtcg gttttggcat gacaagagaa acgctcaatg
    30241 atctagctgc tttaggagtt catacagttg atatcagcgg tcgaagcgga acaagcttca
    30301 cccagatcga aaacgcccgt cgctccaagc gggaactgag ttatttagct gactgggggc
    30361 aatcgacagt ctcttcattg cttgaagcaa atgaagcaga cacttctatg gaaatcctag
    30421 cctccggagg tatccgcaat gcctatgaca tatttaaagc cctttgtctc ggcgcaaatg
    30481 ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa tcacggcgta gaagaaacta
    30541 ttatactgat gaagcaatgg caagaagagc ttcgtctgct ttatactatg gtcggcgcaa
    30601 cgaataccgc ggccctacac caacaatctc tgatcttttc cggaccagtg aaggattggt
    30661 gcgaagcaag agggatcgac ttagtgaagt atggaagacg cacagaaaag agcgtgggac
    30721 aaaagtaaag aacacttttg tctcacgctc taaacacgta caaacggcgg aagtaaaagc
    30781 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaattcc
    30841 ttcttatttc ttggagttaa atgcttctgt cccagcctct tttttcgaat aaatgatgtt
    30901 taatcagctg tccttcggta ttcgttcaat ccgctgattt cacaacctct ccactaaaca
    30961 gtgtactaaa gctcttttaa ggttgttttc cgatataggc aagtatgcct ccgtccacat
    31021 acaagacatg gccgttaacg aaatcagatg cgctagaagc caagaagaca gatggtccag
    31081 ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg tccaacgata aattgatcga
    31141 atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc ggtgtagcaa
    31201 tataacctgg tccaattcca ttacattgga tattgtattg accgtattca gaagcgatgt
    31261 ttttcgtcag catttttaat ccgccttttg ccgcagcata agcactgacc gtttcgcgtc
    31321 ctaattcact catcatggag cagatattga tgattttacc gccgcctttt tcgatcatat
    31381 ccggaataac tgcttttgaa acgatgaatg gggcatttaa atccacgtcg attacttggc
    31441 ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt gatgatccct gcattgttga
    31501 ctagaatatc aactgaacct acatcttctt tgatctgttg aaccatcgct tgaaccgctt
    31561 cttcatcggt cacatcgcag acataacctt ttgcatcgat gcctgcttct ttataatgcg
    31621 ctataccttc atcaactgat tcttgattca agttattaaa tacgattttt gctcctgctg
    31681 tagctaaaga gcgtgcaatc tcaaatccaa taccatagac agcaccggtg actaatgcta
    31741 cttttccatc taaacgaaac atatccatac taaattccat cattttctct cccttatgct
    31801 tattttaatt gatccatttg caccatatcc atgtctgtat aggtgatatt ttctccgcac
    31861 attgcccaga tgaatgagta attgcttgta cctacacctg aatggatcga ccagcttggc
    31921 gaaataatgg cttgttcatt gtctacaact aaatgttttg tttcatctgg tttgcccatc
    31981 atgtggaaca cacgtgtatc ttcattggca taatcaaagt agacataagc ttccatacga
    32041 cgttcatgtg tatgacaagg catcgtattc caagcgcttc ctggttctaa aatcgtataa
    32101 cccatttgta attgacagct ttcgcataca tttggatgga tgtattggta gattttacgt
    32161 tcattcagtg tcaggccttc accagtctcc attggtttta cttggtcgat actgattttt
    32221 acatttggat atttatgatg agctggaaca gaactaatat agaatttagc tgggttttca
    32281 gcatcttgag aagaaaatac aacatgtcgt gtttcttttc cgatatagta tccgtcttgt
    32341 tttttcattt cttctttttg cccatcgatc tcgatgaatc ctggtccacc gatattgatc
    32401 acacctagtt cacgacgctc taagaagtaa tcaacaccta gttctttgtc taatgtgatt
    32461 tctaattctt tttcagtagg tgtcactcca ccaaagatca ttcgatcgtt gtgcgtataa
    32521 gtcaaactga ttttccctgg ttcaaatacc ttttctacta aaaattcttt tctcaattgt
    32581 tccgttgaat aatgacggat atcttctgga ctgtgtgtat aacgtgtttc catttcttgc
    32641 attttcttct actccttcta tcgaatgatt tttccagagc cgcatgctaa aaattgtgcg
    32701 acttcttctt gactaaattg gttgcagtcc ccatggatcg tatgcttcaa ggcagaagct
    32761 gccgtagcaa aatcaatgat ttcttgcgga gacatattgg ataaaatccc atggagcacg
    32821 ccacctgcaa aagcatctcc gccacctact cgatccacga ttggggtgat tctatggact
    32881 tgggattcca catattcatg gttcatccac aatgtcccct tcaattcatt ttcacttgct
    32941 gaatgaacgg tacgcttggt cgaataaaac acctgaatat tcgggaactt ttcctgcatt
    33001 ttttgataat aataaattag aggttgttct tctgcttctg gcgctttctc gatacctaac
    33061 aaataaagag catccatttt tccagctgag cagatatcta ctaaaggtaa aagctgatgg
    33121 attacttcgc tggcttcttg ctgtgtccat aattttccac gatagttgat atcgaagctg
    33181 atcaggcagc cagctttttt tgcagcttct atcacctgtt tcgtcatttt ttgccatttt
    33241 ttagacaaag ctggggtgat tcctgaaaca tgaaatatat ctattccttg gaataactcc
    33301 tctagcgacc attccaaatg atccatttca gcaaaacttg atccggcacg atcatagatc
    33361 actgaggcgg cgcgttcgcc actaccagtt tccatataat aagtacctag tcttgatcca
    33421 ccagataata cctgtttcgt atctactcca taacattgca agtgcttttt gactgctttt
    33481 cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt catgtccgta attagctaaa
    33541 gaaattgcta cgtttgcttc ccctccgcca taatgggcag cgaatcgttc acttgaatcg
    33601 atacgggttc cctgctctgt ggacaatcgc aacatgattt ctccgagtgt cactactttt
    33661 cccataagca gctacctctt gatttctttc atttttgctg catactgttg cgcgacttcc
    33721 gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa ggttcccacc tactccgact
    33781 gttacgacac cagcatcgaa ccactctttc atattgtcca gactcacacc gccagttggc
    33841 atgatattga cttgaggcat cggcgccttg aaggcagaaa tgatacttgg tccataagcg
    33901 cttccaggaa atagtttgac gatatctacc ccgcttttca gcgctgtttt gatctcggta
    33961 atagtcatac atcctggtaa gtaaggaatt tgatacagat tgcacatctc tgccgtttct
    34021 tgatcaaagg aagggctaac aatatactct gcgccagcta agatagccaa gcgagctgtg
    34081 acagcgtcta atactgtgcc tgcgccaatc actatttctg gttggtcttt gtagaatgac
    34141 aaaagttctt tgattacttg atctgcttga gggactgtaa aagtcaactc aataccagtt
    34201 agaccacctt tgatgattgc gtggcaagca ttcaaagctt cttctttgct ttttccgcgt
    34261 accaccgcaa tcactccggc gtttttcaat cgagataaaa tatcaatttt tttcatcttc
    34321 tccacctatt tcctatttag aaattttatt ttataacttg gaaatctata cccatattat
    34381 acggcagaaa agaaaaattg caaacgcttt tttcattatt tttgaattct ttgctcgatt
    34441 tcgctttttg tttgcttgag ggcactgatt atttgttctt ttgtctcatc tgttaaacga
    34501 tatttcggca tacttacact aaatgcacca atgatttgtt cgtttttcac gattgaggct
    34561 cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa atgcgatgtg ggtttctttt
    34621 accgttttga tttccttttt caaccgtaat gcattggtga tcgtatattc tgtgtaaggg
    34681 atcaacggat gcttttcaag ataatcagtg tactgctctt cactgaattc tgctaatacg
    34741 gctttgccca tcgcagaatt gtataatggt cgggtgattc cgatttttga agacatgcgg
    34801 atcgtttgat gttttggatc tagtttgttc acgtacaaga tctcattgtt gtccaatacc
    34861 cctaagtgga tagtctcatc gatcttttgc tgcagctgtt ctaaaaacgg taaagtcaac
    34921 tctaccaaat cgatttgttc aatgttttta ttcgcgtagc gaataagctt tgcacctaaa
    34981 cgatagtttt tctctgaatt tttatttacg tagccgatca tcaacaaagt atctagtatt
    35041 ttcaagactg tagaagaggt cattcccaca ccctttgcga tcgtctgcaa agagacatct
    35101 tggttttccg ctaaatagtc tagtatttta gaggctttga tcaatacagt tccataaggc
    35161 tttttatttt ccatataata ttctcctatt tctttataag aaaaattata gcggaaatac
    35221 catcaattta aaagagatat ccagatataa actaccattt ctctttacag gcaatttaat
    35281 gttgaagatc cgcttcgttt ttacatcaaa ctttgatata aagcaatgat ttcttctact
    35341 gtcgtttctc ttggatttcc tggtgtgcat acatctcgca aagcatcttc tgcaattgct
    35401 ggaatatctg cttcttttac acctaattca gaaatggttt ctgggattcc aaccgctttg
    35461 ctcaaccgat cgatctcacc acaagccgct tctcttacat cttctaaaga catttcagct
    35521 gctcctttaa tccctagaac aagagcgatc tcacggtact tttctccagt atattctttg
    35581 ttatatttca tgacagttgg caaaagtgct gcacacgcta cgccatgagg aatgttgtac
    35641 catgcactta acgggtgagc catcccgtgt accagtccta aaccgacatt tgagaagccc
    35701 attcccgcaa tatactgtcc taatgccatt ttttctcgcc cattttgatc gccatctaca
    35761 gaagcttcca aagaatggcc gatgatttcg attgctttta aatgcagcat atcagtcatt
    35821 tcccaagcgc cttttgtgat aaacccttca atagcatgcg tcatagcatc catacctgtt
    35881 gatgcagcca attttttcgg cattcccatc atcatatcgc tgtcgatgaa cgcgacgatt
    35941 gggatatcat gaggatcaac acaaacaaat tttcggtgat ttacttcatc tgtgatgacg
    36001 tagttgatcg ttacttctgc tgctgttcca gaagtcgttg gtatagctaa tataggtaaa
    36061 catggatttt tcgtatcagc aacaccttct aaactaatga cgtctgaaaa ctctggattc
    36121 gtcaaaataa tcccaatggc ttttgccgta tcgattggcg aacctccacc gattgcgatc
    36181 aaacagtctg cctctgcctc tttaacaaaa gccactccat cttttacatt ttggatcgtt
    36241 ggattaggta cgatcccgtc gtataaagca taagcgattg ctgctttatc tagaagttct
    36301 gttacttttg ttgctatacc gtgctcgatc aatcctttgt cagtaatgac agctgctttg
    36361 ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa tcgctccttt tccaaaatac
    36421 gatgtttcat ttaaaatcat tcgattactc attttctatt cctcctaatt tgtattattt
    36481 ttcacaataa aaacgaaaga accccaagca accggttgcc cagttgcttg ttgctacagt
    36541 tcaaagactg gcatcaattc ccaactgact ggagaggcat ctggatttgt ctccatgata
    36601 tcttccatgt acttccacca tttttgattg atagatgtat ttgccatttt ttcccataac
    36661 ttttcatctt cgatcactaa gtaaccaaat aacaaacttg tactcggatc aagatagatc
    36721 gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca tttctggcca aagttcatca
    36781 tgtctttttt tgtattcttc ttgtttttct gggtaaactt tcatgcaaaa agcttttttc
    36841 accatgtcag acacctgctt tcgtatgaac ttcctccaaa taacctgctt ttggagtgac
    36901 accaaacgca tctgctaaat gccacaggtt ttcgtccgtg attgtttgca agattggtcc
    36961 ttgcgctttg acgtacgtat agacttctgc tgccttttct gctgtctcga tcaaaccaaa
    37021 gacttcatcc atgtcttttc ctgacccgta gattccatgt tgtggccata aaaccaatcg
    37081 agtttctttc attttttcag ctgttgcttc cccaatctca tttgttcccg gaaccatcca
    37141 cggaatgatg gaaattcctt ctggaaaaac aactaagcat tccgtacaca tttgccatag
    37201 cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa ctcatcgcta atagatgact
    37261 agcatggcaa tgcataacga tccgattatc tgggtctact tctaaacggg cgatatgact
    37321 catgaaatga cttggcaact cgcttgtagg aaggcattcg ttttccagcc cccacaaaag
    37381 ttccagtgtt ttgccatctt cagcaactcg aacgattcca agattctcgg caggttcgct
    37441 cgctacattt ttaaaatatc ttcctgaacc agttacaata aaatagcatc ctgctaaacg
    37501 gctggcatca aaaatcatcg agatgcggcg gatcactttt tcaggatcca agaacggtgt
    37561 gatctcttct tcttttagta aataagaaat attcccgcca tttctttcat cccatcccaa
    37621 gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca taaggcgctt gtaaaatatc
    37681 tatttttttc aatatagcca cactcctatt ttcttaattc caacacgtct ttttgatatt
    37741 cctggacttc ttccagccaa tccaaaccaa ctggaacttg atttttgtaa cagaaataat
    37801 tccatacatc ggcatacggg aaatctttca attcttccgt ataagccatt cttgaagtaa
    37861 agtcaaaagc cagctccatt tctttcaatc gctcaactgg ttccagcata gctttcagca
    37921 gtgctttttg tgtatttctc gtaccgatga cccaagcagc gatacggttg atcgttgcat
    37981 caaagaaatc cagaccgata tttgtttttt ccaacaattc gttacggact aattctttag
    38041 cgatttcctg caattcatca tccatcaaaa ccacatggtc tgaatcccaa cgcactggac
    38101 gggaaacatg caacatgatt cctttgctga ataaagctaa tgaagataat ttattggaaa
    38161 tcacttctgt tggatgaaaa tgtccagcat ctagacaaac aagtttgtca cgagtcaacc
    38221 cgtaacccat ataaaattca tgggaaccga ctgtataagc ttcagcgcct aaaccaaata
    38281 gtttgctttc tactgcatct tgtacatacc gttcatctaa ctcttctgta aaaatttcat
    38341 cgagtgattc cattaaacgt tcccgtgggg taaaacgatc aatcggatta tctttcatcc
    38401 catcaggtac ccaaaagtta ttgatacttg tttgcccagt ttctttccca aagtactccg
    38461 cgattttgcg gcttcttttc ccgtgttcga tccagtattc acgaacttct ttatctggat
    38521 gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa gaaagtcgga ttgaagtcaa
    38581 gaccgatttc ttcttttttt gcccactcta cccatttttc aaaatgtttc ggctcaattt
    38641 catttagatc gacagattct tctgtatcca aatagatagc atgaaggttc aatttgtgtt
    38701 tgcccggtat caacgaataa gccttctcca gatcttgtct aagctcttca ggtgttcgtg
    38761 cggctcctgg aaaattccct gtcgccatga ttccaccggt caattcgcca tccggtgtca
    38821 agaaaccttt gacatcatct ccttgccagc aatgcataga aattttgatg tcctgtatct
    38881 tttctagtac agcatctgta tctacaccga tagttgcata tttttctttt gcttgttcgt
    38941 aatttctttc aatcgtgttc atacgattac tcctttgatt ttattcacgt accattccat
    39001 cgtgttttgc ttattttcat ctgggtaaaa agtttcacat ggaaacgact gtttgattac
    39061 atttcttgct tgttctatat tcgaaaactg acctgtactg agcatttgca tggcaacatt
    39121 cccgattgct gtggcttcac taggtcctgc ttctacaatg attccggcat aatctgctgt
    39181 caattgattc agtagacgat tattcgatcc gccccctacg atgtgcagct tttccaattt
    39241 cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg taacaaagag caagattatc
    39301 atacacacaa cgagcgatag ctcccgatga gacagggact ggctgctctg tttcaaaaca
    39361 gtattgctga atctcttcta tcatatttgg gggattcaaa aatcgagggt cattgatttc
    39421 catcacttgc tgcagcggtt tttcttttgc agcaagctct gccaattccg cataactgta
    39481 ctcgtactct tgcattcggg caacttcctg gatcaaccac atccccatga tgtttttcaa
    39541 aaaacgcacc gtgttatggg caccccattc attcgtataa ttttcagaaa aagcattgcg
    39601 agaaacattg gcgatctttg tttcaatccc taaaagcgac catgtcccac tgctgatata
    39661 accccaacgt tctccttgac ctggcgttcc taatacagct gacgccgtat catgacttgc
    39721 tactgtgata aaggtcacat ctggcaaatc atattttgaa aattgttctt tttgcagatc
    39781 tcctagtatc gttcccggct ctgtcaatgg tggaaataat tccttcgaga caccgatttt
    39841 ttcaagaagt tctatttccc attcttttgt ccctgcattc agcagctgca tagtagatgc
    39901 attagttttc tccgtcacca tgttaccagt aaagacatac cctaaataat caggtatcag
    39961 taacaattta tgtgcttttg ccaaattttc tttttcttca gcaaacaatt ggaacaaggt
    40021 attgaaaggc tggatctgaa tccctgtctg ctcatacaac ttatctagag aatagatttc
    40081 agaaaactgt tctactgctc cattggtacg cttgtcccga taagcaatcg gatcatttat
    40141 ccgatcccct tgttcatcaa tcagacagta atcgactgcc catgtatcga tccccgcaaa
    40201 aaggcgatca atccctgtct gctttaattt ttcaaatcca atcaaaatct ctttgataag
    40261 ataatcgatc ttccagcgat cgtgaccgtc tttgttccga aagccgtttt tgaaccggtg
    40321 tacttcttcc aagatcaaag aaccttttgc atccctcgta cttttcatta gtcgtccgct
    40381 tgatgcaccg atatcgatgg caacaaatgt tggattcatt tctccacctc ttttacttta
    40441 ttgttgatta gcactttatt aataaagcgt tatcatttat tgacaatgct atcataatac
    40501 agaatcttat attttgcttc acactatttt ggtatataat tgcattatct tgacaagttg
    40561 ggagtgttta catggattac tgggaaaaag ccaaccactc atggtcggaa gactcaaaaa
    40621 gagtgatctt gacgcctaca caaagaagca ggaatttatt tttttatatc caagaaatcg
    40681 gttacttcaa agcaccaatc ccttactata ctgaacgagc acatttacct tcttacttga
    40741 taaagtttac tttgtctggc aaaggttcgc ttacatataa tgataatcag caacagctaa
    40801 aagctggaga cttatttttt attgactgcc aagaatatca gcgttatcaa acgatcagcg
    40861 aagaaccatg ggaaatggat tggatccatt tgaatggtca gaacgtccaa tcattttatc
    40921 aggagttcac aaaagacggc cataacacct tccattcaac aggagatcca tcagaaaaca
    40981 agatccacca tttgatcaca gaattgatcc acatgcaaca aggcacgaat gcccgtaccg
    41041 atttccagtc ctctgtagct atccacgagt tgctgaatga actgattttg caaaagtatc
    41101 agctagattt cgaagaagag gatattcccg cctacgttta caaactgaag caatacttag
    41161 acgaacattt caaagaaacg attacacttg atcaattaga acaacaattc ttattgaata
    41221 aatatcagct gagcaaagat ttttccaaat ttatcggttc tccaccaatc gattatttga
    41281 tcagcaaaaa actttcttac gctaaagacc ttcttcggta tacagaattt acggttcgaa
    41341 tgatttccga agaaataggg attgagaatt ttgcttactt tagccgcctg ttcaaagcaa
    41401 aaacaggact atcgccgaga gactttcgta aattcagctg attcatataa tatgtacgtc
    41461 attttttcaa aatattttga aatatataga taaaaataaa ataaccgcag tttctgcttc
    41521 tatgtaaaca acagcagcta ctgcggttat tttttagtga tcattctcat tgttcatctt
    41581 caaaatgttc ataatgaacc aactgtttct gtgtatcacg tttcaaaaat tctttaggcg
    41641 ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg ataagaagaa aaaccacttt
    41701 tactatatac agcactagag ctgcggtatt ctcgcaacaa gtgcttggca tagatcagct
    41761 ttttcttcat tacatactga tgaaacgtaa atccagtgta ctttttaaat tctcgtgtga
    41821 tatggtattt gctgacaaaa aactgctttt ccatttcctc taaagaaagc ggatgagata
    41881 aattttttga gatatattgt aacatttgtt ccacacgctc attaggaact acagcattct
    41941 gttcttgcgc actctcttct ttttgtacaa cttgattgag aaaaatcaag tattctacca
    42001 gagcttgttc atattttatg tcttcaccgt aaattttttt atcaggagga tggtcgataa
    42061 atgataattt ttgcttcaat acttcctgtt ctatttgcaa tacacgactc ctcttctctc
    42121 ccattggttg aaaacaagcc gatagattag accatggcgt ggaacgactt tctagaaaag
    42181 aaggggtgac aaagatatag acacgctcga atacatctga agattggcgg acgatacgat
    42241 gcaaatcatt ggaatggatg agcagcacac ttcctgcttt gatcgtaaat tgtcgtccgt
    42301 tcaaataaaa agtcgcttct ccctttaatg tagcatggat ctcatagaaa tcatgatgat
    42361 gatagatcgt tttattttgg ctgacgacat ctttgacatg atagatttca tactgatcag
    42421 aaaccatcgt attgacttgt tcaccgctga ttttcttttg gagcatgtgc cgcacctcct
    42481 tttgttcact tatgcacaat tttattatac cacgtttttt gtaatcaaaa agagctgctt
    42541 ttatatactc ttataaaaaa gaagagacgt ggcatcttct agccccgccc cctccattct
    42601 aaatattagt taaccaacgt cttctttaca ttattcaaga agacttcttc cataacgaat
    42661 cccatcatct tttgtttctt caagaaccac atacaggtac ggacgattct tcatgatgat
    42721 ttctttgatt tttgtgtact cgatcacacc gtctcccaaa tttaccggca cgattttttc
    42781 ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc ttttcaccaa aacgttcaaa
    42841 agcttcttct accaattgga cttgttcttg ataatcacta cttgtgatca aattggtcgg
    42901 atctaaaatg attcctagat agtccgaatc tactgcttgg atcagctgct ctacccttgc
    42961 taaagaatac accggatgat tcaaacctgg ttctatcccc accatcattt ggtatttttc
    43021 tccagctttt accaagcgac ggataacgga cacagcttca gcaaatgctt catctgtgaa
    43081 attttcttcc gtatattgga tttccggcaa aacacatcca gtttccgaag caaccatcga
    43141 cgcaccaaag gaagaagcat agcgaagata ctgctcgaac tttgtcagca actcttgtcg
    43201 tatcgttaaa tctggatgaa tcatattgat atagcaactt aaaattccta cggtcacttc
    43261 ttgcttttct aaaactcgtt tgacataatt tcccatccca gaattgatct catttgcgcc
    43321 agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc agtgtatgaa tcccttgttc
    43381 ttttgttttt tctgcaagat cttctatcgt ttggacttcg gaaagatcat gtcctctaat
    43441 tgtaatgttc aacataacta ttctcctaac tcagcatatt gggtagccac atgacgattt
    43501 gcgggaagta agtaatcaaa aacaagacga ctaagataac cccataaaaa ggaagaagcg
    43561 gtttcagcat tgcctctgct tttacgcctc ctacacttgc accgacatat agtcctgtac
    43621 ctactggcgg cgtgatggag cctacacaca gattcatgat tgaaaacaac ccgtaatgta
    43681 caggatcaac ccctacactt gtgacgattg gtaaaaatat tggtgtaaaa atcaagatag
    43741 caggcgccac atccataaac atacccacca gtaataacac aagattgact aataacagaa
    43801 taataaattt attatccgtt acgcccataa tcaacgaact aattgcctga ggaataccgg
    43861 taaacgccat ggcgaaagac atcatggaag aagtagccaa taagaacatg atcgtccctg
    43921 acattgctac tgattgaaac aacatattag gtatatcttt tagttttacc gttttatagt
    43981 aaaacatagc aatcaacagg gagtaaatga cagctatcgc tgaagcttct actgctgtaa
    44041 atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta ggaatcgcct
    44101 cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg tatttattct
    44161 ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaagcg cctacgagat
    44221 aacctcccat aaacaaagca gagatcgaag caccaccagc aacaagtgaa tacatgataa
    44281 aagcagtact tggaggaatg accatacctg tcggagctga cgcgatattg accgctgtcg
    44341 caaacttccg gtcatatcct tcttccactt gctgagggat caacactccg ccaatcgcgg
    44401 tagaagcggc aatggccgag ctagagatgg aaccaaataa agcattccct aacacattcg
    44461 tatgagccaa agcccccggc acgcgcccga cgaacaacat cgcaaaatcc actagtttct
    44521 tagctatccc tccattattc atgataatgc ccgagagaac gaaaaacgga atagcgatca
    44581 gcgaaaaact attgatactt cccaccattt tttgagctga actaaaagca gaaacatcaa
    44641 acggtataac taacaataaa gtcatcatcg atgagaatgc aatacttata gcaatcggca
    44701 tccctaatac aagcaacaca acaaatacaa aaaataacac taatccggct tctattgcca
    44761 ttttattcct ccatttttat attttttctt gaattgctgg cttgttgctt aatgtatcga
    44821 ttagattgat caggctatag ataatgatga gtacccccga tacaggtact gacagataaa
    44881 catatcccat aggaatactt aaagaaggcg agatctgccc cattgtcaat gtgacagcat
    44941 tccatccgcc atacaccatg atcatcgcag caaataccag aaataaactt tgaacaacaa
    45001 cttctaaaat tttacttttt ttgccagtta atttttcact taaaaccgtt acggctaaat
    45061 gtgctttttg tcccacaaca tacgcggaag acaacatggc aaaccagaca agaccgaatc
    45121 gaacaagctc ttccgtgacc gtatttggat ttcctaaaac cgttctggaa aatacttgcc
    45181 ataaaacaac agcgaccatc ccaacaagca aaacagctcc caggatctct aataccttgt
    45241 ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg atcttttgat aaaggtcttt
    45301 gtaggtagga tcgtttttga atatatcatg aagtggggca actgcctctc gaaacggtgt
    45361 attatctaca tcattgaatt ggactccatg ttcttcttgt gctaaagctt tttcttcttc
    45421 aattgctttt gcaaaagctt caatttcaaa aaccgttgat tctttcgctg cttcttcaat
    45481 ggctttttcc tgctcatcag ttagctgttc tttgatggca tcattcatga tgatgatatc
    45541 tggcacacgt gtatgctcat cataggaata gtatttagcc acaccaccat gtccagctgt
    45601 atacaagaca aattcattgt tttctgatcc gttgatcaaa ttggattgaa gagacgtgta
    45661 cacttcatca cttcccatag gtacgggcga accgcctaaa agctctacca ttttgatagc
    45721 ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa tcttccggcg tatggatcgg
    45781 accgtctacc atataaaagc tgcgctgacc agaatcataa taggtcaatc cggtaaatcc
    45841 taaatttttg gtcgattggt agattggctc cattatttct tcattatcca tcaccttaaa
    45901 gaaatgttct tcatcattga atagataagg aactgaaaaa atcgaataat ctttagagaa
    45961 actttccaac gctgaggcac ttacttttgt gaaatctact gctcctgact gtgtcaattc
    46021 gatcagttca cgttctccac ccagttgtcc atctgggaaa tactggatct gtacggaacc
    46081 atctgttttt tcttttacta aacgcccaaa ttcattcatt gcatcaatta ctggctggct
    46141 gttgcttgca taagcaaagc gcaaggtaac tatctcattt acatctgcat ctgcgacagt
    46201 tgccacacta gaattatttc cgcagccgct tagacctagc agacctaggc taagcgaact
    46261 tgctgcaaac agcagtgatg tttttttcat atgggtttcc tttcttttaa caataagaga
    46321 cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt gctgtgattc
    46381 cgatattccg ataaaaatct aaactgtccc ccattcctgt cccgattgaa acgttttgaa
    46441 cttctccttt ttcatcgatt tgttcaagca atccttggat agcacgataa gcgacttctt
    46501 tatattcttg tggtaaataa cgtttatgga ccgctttcaa aataccgtaa gcaaatcctg
    46561 cagttgcaga agattctacg taagaagaag gatcatccaa caacgtatgc cataaaccgc
    46621 tttcagactg ataagttttt aatgcacgca cttgtgcttc aagtgtttcg atcaataatt
    46681 tgcgcaatgc atcttctttc gataattcta agatttcaat gatctctgga atagcaatcg
    46741 tcaaccagca gtttccacga gcccaaaatg cttcggcata gttatgattt ccttcaaacg
    46801 tccagccgtg ataccacaat cctgttcgtt tatccatcaa gtatttgata tggatcataa
    46861 actggtaacg agcttcttct aaatattctg ggcgattcaa cagtttcccg atttttgcca
    46921 aaggaagcac tgtcatcatc aatgtatcgt cccacaactg attcttattt tccggtccat
    46981 atgtggcatg ctgtaacccg tcttcatttg tccgaggcat gtcattcatg acccattctg
    47041 cccattgttc taaataaggc aaatagcgtg aatctttcgt gtcttcatac aagtaagcca
    47101 tagtcaatag cggtgccatt gtattgacgt ttttgggcgg agcgccttct tccatacgat
    47161 cctcaaacca ttcattgacc acttgataag cgcgaggatt ttttgtcatc tggtaatttt
    47221 tataaatacc atatagaccg acaccttgtg gccaattcca cactgtccag cttttgtcat
    47281 ctactttcag tccatcaaag tccaacaaaa attcaccatt atcgtctttg atcgttgtca
    47341 ggttttcaac gagcaggtca attttttctt tcactaattc tttaggtaca aaaatctctt
    47401 ctttaataat catctttacg ccaccttttt aaacgctttc attttatatc cttaatatag
    47461 cagtaatacc gtccactata aatatcgctt tttgaacaaa aactgtctaa aattgaccta
    47521 aatgttaaat aacaaaagta actttcagtt acttggaaat taaaaaaaga acttggagaa
    47581 acaacgtttt ccaagttctt tttcatctat tttcttatct tttttctacg ctttcttttg
    47641 cttatccgtt gtaagcatca atgatgatct gtttcaattc agagatcaat ggttctttcg
    47701 gattagctgt cgtacattgg tcttcgtaag ctaattcagc catacggtct acggttgtat
    47761 ctagtgtttc ttgtgtgact ccttgagctt ttagactcat gttgatgccg acagattttc
    47821 ctaattcata aacagccgtt gctaaagctt cgactaattc ctctgttgtt tcgcctttca
    47881 gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc acggaaatag tcatatttag
    47941 ggaacatcgc atgtttttgt ggatcttttg cattatagcg gataatatgc ggtaaaagaa
    48001 tcgcatttgt ccgtccatga gggatcccgt attccccgcc gattttatgt gcgactgagt
    48061 gacaaatacc taggaatgcg ttagcaaacg ccataccagc cattgtagaa gcgttgtgca
    48121 ttttttcacg tgattccata tcaggtgttt ttactgattt ttctaaatag tcaaagacta
    48181 acttgattgc ttgtaggctc aatccacgtg tataatctga agccatgact gaaacatagg
    48241 attcgattgc atgtgttaac acatccatcc cggtatctgc tgtgacagat gctggaactg
    48301 acatgacgaa ttgaggatcg acgattgcga catctggcgt caatgcataa tctgctaacg
    48361 gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact tcagaaccag
    48421 tccctgatgt tgtcgggata catacgaatt gtgttttttc tggtttgtcg atcttatatg
    48481 tccgtttacg gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg tctgggtgtt
    48541 catagaacat ccacattccc ttagctgcgt ccattgcaga accgcctcca agagcgatta
    48601 ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc gtatacggta tttgtcgatg
    48661 ggttcggttc aacatctgag aagacttcga tcttcacatc atttttacgt cgtgccaata
    48721 cttcacgaac tgtatcgcag taaccgaatt ggaccatacc agggtcacaa acgatcatga
    48781 cacgttcaac gttttccatt ttctctaagt acaatagaga gttcttttca aagaagattt
    48841 ttggcggtaa tttgaaccat tgcatgttat ttctccgttt cgctacagtt ttgacattga
    48901 ttaagtttac agcagagacg ttacgagaaa cagagttctt accataagaa ccgcatccca
    48961 aagtcaatga cggaatcatt tcgttataga tatcaccgat gccgccttct gcagatggtg
    49021 tattcactaa aatacgacaa gctttcattc gaagaccgaa tttgacatgc aactcttcgt
    49081 tttctgaatg gataactgct gtatgtccta atcctccaag attcaacata ccttcacaca
    49141 aatcaaacgc atggtccgtg ttgttagctt tgatcatggc aagaactgga gaaagttttt
    49201 ctcttgatag cggataatct gggcctacgc cttccaattc agcaatcagc attttcgttc
    49261 cttttggtac actgattcct gccaattttg cgatttccat cgcagaatgt ccaacgattg
    49321 atggattgac cgcatatttt ccttcgttca tcactgcatc ttctaatttt tgcaattcat
    49381 ctggttttac gatataaact tggtgtgctt ggaattctgc ttttactgct gcatagattt
    49441 ctttatccac gatcacagct tgttcagaag cacaaatcat tccattatca aatgttttcg
    49501 aaacgatcaa atcgttcact gcacgtttaa tttttgctgt tttttcaata taagctggta
    49561 cgttacctgg acctacacct aaagctggtt ttcctgtaga gtaagcagac ttgaccattc
    49621 cagctccacc tgtcgctaag acgatcgcga tacctggatg gttcattagc atagaagtcg
    49681 cttcgatcga aggatgttcg atccattgga tacaattttc aggagctccg gcagcgatag
    49741 cagcatcacg taccactcgt gctgcttctg cagaagattt ttgggcactt ggatggaaag
    49801 caaaaacgat tggatttctt gttttcaacg cgatcattga tttgaaaatc gttgtggaag
    49861 tcggattcgt tgttggagtt actccgcaaa ccacaccgac tggttcagca atttcaatca
    49921 accctttttg gacatcttca ttgatcacac caactgtttt atcatgtttg atattatgcc
    49981 aaatatattc ggacgcatac atatttttga ttgctttatc ttcgtaaaca ccgcggcctg
    50041 tttcctctac agccatcttc gctaatggca tatgttgatc caaagctgcc attgccattt
    50101 catgcacgat atggtctact ttttcttgat caaagctttc catttctttc aatgctgcat
    50161 ttgctttttc tgccaaatca tcaatcattg cttgtacgtc aattgtcttc gttttttctt
    50221 tttctattac tttagccatc attatcctcc taatgggtca tggttatttg ttaaataatt
    50281 cacatgtata tcatacaata tttatttttc tatgtaaaca cttttttgtg atttttttca
    50341 cagttatttt taaatataaa attgctttat ttacaggtgt ttgtatagaa aaatatataa
    50401 agttatttat tattatttat atgacacacc cgattattca atattgagca atatatccca
    50461 tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc tgccctacct
    50521 cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc ttccagcgtt
    50581 tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt ttcaacagta
    50641 gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc tgcacgatcg
    50701 tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct
    50761 gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt catgctatct
    50821 aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt
    50881 gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt cacctcacta
    50941 attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag ttttttactt
    51001 aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa cccgtattct
    51061 tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt
    51121 ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat cccaaatgtt
    51181 tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca tgtataacaa
    51241 tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt cactactaga
    51301 cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa gacacagtca
    51361 aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc gacacccatc
    51421 aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac acgattcatt
    51481 tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa atccatcccg
    51541 actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg gatgattcca
    51601 aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc ccatcgagac
    51661 gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg acattcgtca
    51721 aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt ttctggtgat
    51781 aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc ttcgatattc
    51841 cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc
    51901 cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc agggcgtagc
    51961 cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa ctcttctggg
    52021 gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt ggggaaagtt
    52081 ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt cttgattaaa
    52141 cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa atatcttgac
    52201 aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc ttccttctac
    52261 tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa gaaaaacatt
    52321 atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa ggagggaaaa
    52381 atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa aggacgttgg
    52441 ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac tattcttctt
    52501 attatgattc ctattatctt gtttgccacc accatatatg atggatcagc gagcgtctca
    52561 gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat ttccagcatc
    52621 attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt acgtggacaa
    52681 aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt gtttatcggc
    52741 ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg tttgctatta
    52801 gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat ttactacgat
    52861 atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc aagtcgaaaa
    52921 ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat cggctggcat
    52981 atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata catttctgca
    53041 acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt gtgacttgtg
    53101 accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa cattcagccc
    53161 attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta tagttccagt
    53221 aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc cggtaatcga
    53281 tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc taccaagaac
    53341 cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg cagcaaagca
    53401 acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt attggtcgct
    53461 aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag aatcgatgaa
    53521 taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc tacccatggg
    53581 ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat ggtgaagacc
    53641 cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc atttttcgga
    53701 atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc aaatcctgga
    53761 agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg tgctccccat
    53821 cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct tagcaaaaag
    53881 ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc gtctacgatc
    53941 gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac atctgttcct
    54001 gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc attcacacca
    54061 tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat ccggatcttg
    54121 tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc taattcttcg
    54181 tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt caagatacct
    54241 aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat gactgttttg
    54301 atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg tggatcgatc
    54361 attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga aatatcttgc
    54421 ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg tttaccaaag
    54481 cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca
    54541 tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac tgttgtcatc
    54601 atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc ttctaattct
    54661 gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt tggattacct
    54721 ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag tgcagccatt
    54781 ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac ttcatcttct
    54841 ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag
    54901 cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat gatcgcatgt
    54961 ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac agtcaaggtt
    55021 tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag gagtggttct
    55081 ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat catactgatt
    55141 cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt attttcgttc
    55201 aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac gatcgctttc
    55261 ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc acctaatgac
    55321 tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc tccagtcaac
    55381 gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc agaaatagca
    55441 tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc aacagtggtc
    55501 actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa ggattccagt
    55561 gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac aacgatagcg
    55621 atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt ataaaaggaa
    55681 atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt
    55741 ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt ttttcgccgt
    55801 tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac cttctttact
    55861 tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa
    55921 aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa gggcttatcg
    55981 attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga
    56041 tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga atttctcgat
    56101 agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac atgcgtgtag
    56161 agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca
    56221 agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg aataggaaca
    56281 agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa
    56341 ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt tatttgaatc
    56401 cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt gtcataaaat
    56461 ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta tttcacgaac
    56521 atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat
    56581 aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga ttttggcaaa
    56641 tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt aatgaaaata
    56701 tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat cgtttctaat
    56761 gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg tacttggttc
    56821 aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg gtgttcttca
    56881 attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt cagatagact
    56941 aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg caatccagct
    57001 gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg atctgggtct
    57061 tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc aagaatctct
    57121 agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc
    57181 actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt ggcacgtttc
    57241 gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag aaggacttct
    57301 aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac tcgtgtatta
    57361 ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa atgtttatct
    57421 tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg gtcttttaat
    57481 ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt tgttaacata
    57541 tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt tctgttggca
    57601 aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact accctatttg
    57661 tattttttca ttgttttagg catactagta atagatagtt cttcattgaa aatagaaaaa
    57721 gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg
    57781 ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt atcggtacga
    57841 gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat ccaacacaag
    57901 ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa ttgcgacgtc
    57961 agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc cgtgaaaata
    58021 tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa acagctgaaa
    58081 aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca catgaattat
    58141 ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat caagatatca
    58201 tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc
    58261 ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc catgacatgg
    58321 atgaagcgat taaactagca agccgtatcg ccatcatgag tgaaggccgt gtgatcaatt
    58381 tgatacacca gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg
    58441 agaagatcgc ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa
    58501 tacagcaatc acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga
    58561 aaaacgtgta gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga
    58621 tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa
    58681 acatgttttt tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa
    58741 acgcgggctg aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac
    58801 tcgtgcatca ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac
    58861 tgatgttgca gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt
    58921 ttttcatcag cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg
    58981 acttgctttg atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt
    59041 tccaaaagct gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct
    59101 tgctttattg gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc
    59161 actctttatt tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt
    59221 cgattggaat tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt
    59281 ttccgttgaa ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt
    59341 ttacgtgatt gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt
    59401 gatttttagt ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt
    59461 aacaatttta gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc
    59521 gatcgcactt agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt
    59581 ttctctcttc ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa
    59641 gacaccattt cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt
    59701 gccggaatgg ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact
    59761 acaactatca accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac
    59821 acaggaacag atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg
    59881 tttgctaccg tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat
    59941 agttttgaaa atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta
    60001 tctaaagtca gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct
    60061 tggatcaatc gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc
    60121 aattcgatat tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg
    60181 gatattgttc ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg
    60241 gaagatgatc gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata
    60301 ttagctgata cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg
    60361 gaaaaaatgc aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta
    60421 gcacaacgtt tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa
    60481 tgaatgtctt tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac
    60541 aatttatccg tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga
    60601 ttccggttgg gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg
    60661 cgaatatcat ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag
    60721 gcctgggagt caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca
    60781 agaatacgta taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca
    60841 tggggatgac tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta
    60901 tcatggcagg cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat
    60961 tcgtcggtgc tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca
    61021 catcgattat tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc
    61081 ttttaggtat tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt
    61141 gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca
    61201 tttttaaggc aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct
    61261 tagctattta tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat
    61321 ccaccgacta agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac
    61381 tcgtcatgga attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg
    61441 gcatcaattc ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga
    61501 ttgttcgatg ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat
    61561 catcttttat acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca
    61621 ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga
    61681 ctgcatccgt cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg
    61741 taaaccaatt gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc
    61801 cctcgtgatg cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact
    61861 tcttccgttt gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa
    61921 ggaacgacac tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa
    61981 tcctctggga accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt
    62041 ttggctgttt ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg
    62101 atggtcgttg ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc
    62161 acacttcttc ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa
    62221 tgacccttca aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga
    62281 aggttggttt tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct
    62341 aaaaaggttg gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa
    62401 ctttattttc ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt
    62461 atctgccttc atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt
    62521 ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc
    62581 ttaaatccac tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc
    62641 ccaatcaata tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt
    62701 atctaaagta cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag
    62761 cccaatcaat acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt
    62821 ttcatttacc tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt
    62881 tgtattagcc attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta
    62941 cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga
    63001 gaaaaagacc cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac
    63061 tagcggtagc caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat
    63121 ccaaagcctg tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga
    63181 cttgattgat atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa
    63241 aactgatttt ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct
    63301 gtcattcatg ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca
    63361 attaaaaatg gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc
    63421 tacttatgaa gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg
    63481 aggctgtaca ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat
    63541 gttttacagc taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc
    63601 ttatagaggg cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga
    63661 gctagagggt ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg
    63721 tgctgacaac gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt
    63781 tatggagaat attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg
    63841 tatagttttg tttcgctgca tttttactta cgatatctac ataacggata attggatttt
    63901 caatcgagcg ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa
    63961 attctgcttc gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa
    64021 cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat
    64081 tcccgactaa tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta
    64141 aacgaagctg ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa
    64201 cgattggcat aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg
    64261 caatgattgc atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc
    64321 tttttccttc ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga
    64381 acacgccgat agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca
    64441 atacgatcga acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac
    64501 gatcttgata catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa
    64561 aagcagaaaa accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga
    64621 cgtaatacaa aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt
    64681 ctttttcagg aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac
    64741 ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa
    64801 agaatggtac agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa
    64861 atgctcgtaa aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag
    64921 cttgcaatgg cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga
    64981 tcacaaggat aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac
    65041 gaagattcag catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt
    65101 agagtgctgg caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg
    65161 tcaacatgta gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat
    65221 tctcactgct gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat
    65281 atgatagcgt taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc
    65341 agatggctgc agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa
    65401 tagatgataa cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct
    65461 catcattttc agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt
    65521 tctaaactcc tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac
    65581 aagatgttat agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga
    65641 aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat
    65701 ttttttatgg gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat
    65761 caagttctat cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac
    65821 ccatcattct tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt
    65881 taaaagcttg catctgcggt aataagccca accaaattaa aaacataaga agctgttttt
    65941 atttggtcgt tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt
    66001 gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt
    66061 ttccattttt ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat
    66121 gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg
    66181 gatagattgc cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg
    66241 ctttcgtagc atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat
    66301 gagtaaatgg caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat
    66361 agttcccgcc tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga
    66421 cattatcgaa taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga
    66481 cagcaaatga cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc
    66541 ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa
    66601 cgaattgttc tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg
    66661 tagcaacact tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg
    66721 tgctagctga accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt
    66781 gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta
    66841 gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga
    66901 tatttggcca gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg
    66961 gcattttttc atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat
    67021 cattttggat aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt
    67081 tgtataggtc tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca
    67141 ttgtattggc atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta
    67201 aaattgccac tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga
    67261 tctggtccgc tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta
    67321 gtagagaagt gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga
    67381 tattattaac aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat
    67441 tttgaacagc tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta
    67501 attgatttag ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt
    67561 tagtcaggaa ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa
    67621 agccgttcaa tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa
    67681 tagctgttct tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact
    67741 tcaatgtaac atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac
    67801 ctgattttgt ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca
    67861 agatatcttg tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt
    67921 tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat
    67981 tttggatctc tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact
    68041 caggcatttt gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt
    68101 aatccacgaa ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta
    68161 attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa
    68221 tcatatcttt aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat
    68281 tgaatacttt caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt
    68341 ggatcgaact tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta
    68401 ccgtatattc gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg
    68461 agaaatcctt tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta
    68521 attgttcttt tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga
    68581 tgacttgctc cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt
    68641 aaacagcaat tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa
    68701 accacgcata tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt
    68761 ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca
    68821 tagttctccc ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt
    68881 tcaatcggct tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg
    68941 taatctctta catattgaga aataattaag gattacagcc tttactttat catgaaataa
    69001 aagggaaaga taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta
    69061 aaacattttt tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg
    69121 tatttcttat ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag
    69181 ctgatgtgct tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg
    69241 aaaggctcct ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc
    69301 ctttcttgga acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg
    69361 gaggtcattt aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc
    69421 tttgatactt cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc
    69481 tggatttgtt ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc
    69541 atttttactg gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag
    69601 acttgacccg accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc
    69661 cgtcaaatgg ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc
    69721 ttcccaactc attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc
    69781 tccgtaagtc ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac
    69841 atctgaacca atctctgtca caataccagc gccatcatta cctggtacat aagggaattt
    69901 caatgttcgt atctctttca tttctcctaa acgcaaggca acatcataag gattcacact
    69961 gaatgctttt atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat
    70021 ttcttcaaaa acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac
    70081 catctccctt tttctttaag tttacgttta tgcctttatt ttgacaaaaa aaatgtcttt
    70141 tcgttttggc gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt
    70201 atttatctaa aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg
    70261 ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc
    70321 tatgctgctt tacgccagga attaaaagcg caaaaagatt acgaaggctt gcgtaaactt
    70381 cctttagatg ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc
    70441 ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt
    70501 ttgattccag gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag
    70561 gagggcgcat ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga
    70621 aatgctggta ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg
    70681 taaaggctgc atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac
    70741 ccacactaat tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga
    70801 ccttttcttt caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt
    70861 ctctttctat tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag
    70921 ccatttttgc cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat
    70981 tttgaataac caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat
    71041 cagcaaaata aatatctcct ctttttacca taggatcctc cttattcttg atattctctt
    71101 ggtatccgct gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct
    71161 atatcctgca tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg
    71221 acaggaaatt cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt
    71281 tcacagtagt tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct
    71341 gcataaccga tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca
    71401 tagccgattc cttcacctgc tgggagtttt ttgacttgaa tcaattcaga aaccagctca
    71461 agtgctggct gcaatggata aacttccggt agtgcgtgcc cagagggatt aagtccgtac
    71521 attgctactc cataacgaat catattgccg attgtttcat catgccataa agccgttgca
    71581 ctattgctga catgtacata tcgaggaagt tcgggaagtt ttttcagcac ttctataaag
    71641 cgttcttttt gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta
    71701 aataaacctt cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact
    71761 tcttcaggtg tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa
    71821 agaggcgtct ctagcttact tttctctaaa acagcagctg ctgcttgcaa ccattcccgt
    71881 gtacctgcag ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc
    71941 atattcaaga taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct
    72001 aaatttgaaa cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata
    72061 gctccatgac cgtatccgtt cgcttttact actgcaaaca attcttttcc ttgcggaagt
    72121 cttgcaactt catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt
    72181 gggcggtgcc aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt
    72241 gcaaaagctg tttcatttgt atgagtaata ctgacccata cattgccttc atgaggtgaa
    72301 cgcgtcacaa caggctgacc attttcattt tttaaaatct cgacatcttg aaaggtaact
    72361 tttccgatcc cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct
    72421 aaaaattcta cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc
    72481 aagattcgtg caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg
    72541 actgcatcga tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca
    72601 ctctcattct accaaattat aaccattttg ccacaaataa gtccacaaat aacaaaaatg
    72661 aaagtttctc ttaaaaaaga gaaactttca ttttatgttt aatcgttatt tgaacggatt
    72721 acaaatccgc gttttttatc attgtgacgt ttaccgccat ctttttggct gttattattt
    72781 ttgctataac cgccttttga tttgtttcca cgataattac cgccgtcacg acgattacgg
    72841 ttgtttccgc caccacgttt gccattttta ttgtatcctt ttttctgcat tggcaatggg
    72901 cgttctggtg tgatcttcac tggcactgca tcagctggat cttttgctgt cgtttttagc
    72961 aaaagtgcaa ctaaatcttg tgcagaatat tcttctaata atttatctgc tgtttgcagg
    73021 tatttatcta gaccattttc tgcaagtttt gtttcgattt gttcaactgc agcacctaat
    73081 tgacctttga atgcttcttt ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc
    73141 aagttttcaa tcacatgcaa gtagtccatt tcatttggtg taacgaaagt cacagacata
    73201 ccgccttttc cggcacggcc tgtacgtccg atacggtgaa cgtagctttc tggatcttga
    73261 gggatatcat agttgtacac atgagtgaca cctgaaatat ctaatccacg tgcagcaacg
    73321 tctgtcgcta caaggatatc taaatgaccg ctcttaaatg aacgcaatac gctcattcgt
    73381 ttttgttgag acaaatcgcc atggattcct tctgcacggt aaccgcgtgc ttctaatccg
    73441 cgtgccaatt cgtctacacg gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt
    73501 tgaacatcaa acaagcgagt catgatatcg aatttttcgt attcttttgc gcggacatag
    73561 tattgatcga tcaagtcagc tgtcatttct ttcgctttga tcttcacatg atgtggattt
    73621 ttcatgaatt taacgccaat attcttgata gctggcggca tcgttgctga gaaaagtaat
    73681 gtttgtcttt gatcaggaac ttgtgaaatg attttttcga tatcttccaa gaagcccata
    73741 ttcaacattt catctgcttc atccaaaact aatgtttgaa cggttccaag tttcaatgtg
    73801 tggcggttaa tgtggtcaag catacgtcct ggtgtaccca caacgatatg tggacgatct
    73861 ttcaagccac ggatctgacg gccaatatct gctccgccgt aaacggcttg tacacgtact
    73921 tttttgtcgc gccctaaacg atataattct tcttgtgttt ggatagctaa ttcacgtgtt
    73981 ggtgcgataa ctaaaccttg taattcgtga cgatctggat cgattttttc taacattggt
    74041 aatccaaatg cagctgtttt ccctgtacct gtctgtgctt gtccaataac gtcttttcca
    74101 gctaatgcta gtggaatcgt ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt
    74161 tcgactgatt tcaacaattc tggtgataat tctagttctt taaatttcaa atgaatcctc
    74221 ctatatggtt gttaactgag gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa
    74281 agtgtattgt ttctttttga cagtggccac gcacgttttt atccattctt agcccctgtg
    74341 gcaccaccga aaaatcactt tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt
    74401 cagctttatc attattatga catctggctt caacaagttg tttctatggc aaaaccagtc
    74461 caatttctct tcatctttcg gcaataggtt attctaacat agtttttcgt tttctgcaag
    74521 tttacagatt tttatttcat ttgctgaagt tgttctacca cttcaaataa gcccatgctg
    74581 ttgctgcctt ttagcataat actatctgtt gcttcaagat tttgtttgat agcatcaatc
    74641 aatttattct tttctttttc aaaatagaac acaggtagtt gggggtattt ttggttcaat
    74701 tcttctttta gtgcatgcat ttgttcccca taaaggaaaa tcatggcgaa tttctcagga
    74761 tcgatatgtt cactcatttg cgcatgcata tttaaagact ctttccctaa ttccagcata
    74821 tctgctaaca cagcgattct gcgtcctttt gttggcagat ttttaaaagt atctaatacc
    74881 agtcccatgg ctgttggatt tgcattgtag acatcgctca aaatagctgc accatttcct
    74941 gccgttagcc attcggttct gttttgagtg atagaaacat gggcgagccc ttggcgtatc
    75001 tcaggtatgg acagtccaaa gaagcgcccg atcgtatacg caatcaaagc attcgttaca
    75061 ttataacttc caggcagcgg gatcgtaaat gtttctcctt ctactacaaa atcggtttgt
    75121 gcttttcctt ttgctgtcac ttccgcatga acatcgccat ttccaatacc gaatgttgtg
    75181 actgtttgta ctaatgttgc cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg
    75241 agtaatccgt ctgatacaag tccgtcagtg atttccattt tggctttagc gattccttct
    75301 cgcgacccaa gattttctat gtgtgcttca ccgatcattg tgattgctgc agcttttggt
    75361 tgagccataa gtgaaagttc cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg
    75421 atcttttctg tatcttgggg catgtgcaaa atcgtgtaag gaagaccaat attattgttg
    75481 taatttcctt gcgtcttata tgttttgtat ttttcagaca agacagacgc ggttagatct
    75541 ttcgtggtgg ttttaccatt gcttcctgta attgcaacga cttctgcgcc tactttttcc
    75601 aagtaatagg ttgccaattg ctgcatggct ttcaaaggat ctgctacagg aataactgga
    75661 aaattttttg gagcttgttc aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg
    75721 atcgcttgct ggataaattc atgtccatct cgcgtaccag caagaggaac gaataaactt
    75781 ccttcggtta tcttccggct gtcaaattcg acattggtaa tctttgtcag tgcagagcat
    75841 tcgctgccca cggcttgtgc aatttcttgt attgttagat tcattgagat ctctcctaaa
    75901 aaaggttgtg aaaaaagcgt ttggcctaaa cggcgggttc gcatgactct cacttccaag
    75961 taaatttctc tattcgtatt tagaagctag aatgaacgcg gttacgcaat cactccagcc
    76021 tcttctttat ttctctttat tcattttttt caaaaaaaga ttgacgctga tggtatcgat
    76081 tcattcctaa ctggatcagt tcttcaatca aatcaccgta tttcaagccc atattttccc
    76141 ataagagtgg gtacatactg aactccgtaa atcctggcat agagtttaat tcattcagga
    76201 ataattcatt tttatttgtc aaaaagaaat cgcaccggct caatccgctt ccacctaaca
    76261 tcgtgtaagc taacttcgcg tactcttgcg ctttttgata aacttcttct ggcacttcgg
    76321 ctggaatctg catttcgatt ttattattga tatattttgc ttcataatca tagaatgcta
    76381 cgtcttttac gacttcacca ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa
    76441 cttcgatttc gcgcgcttca attccttgtt caacgattgc tcgagaatca tactgatagg
    76501 ctgttgctaa agcattttgc agctcttctc ggttttctgc ttttgtaatg ccgacactag
    76561 aacccatatt cgccggtttg acaaacatcg gataaagcaa agaaccttca cattgatcaa
    76621 atactttttt aggattttct ttccattgat tcttaagtac tggtacataa ggaacttgcg
    76681 gcacaccagc agcttgtaaa atatacttgg tcatgatttt atccatggca catgcactgg
    76741 tcaatacgcc tgcgccgaca taaggcatat tcaatgtctc taagaagcct tggatcgttc
    76801 catcttcccc gtttggccca tgtaaaactg gaaaaacgat ggctccttct tctttgattt
    76861 cgcccggatt gatcactttt cctgtaaagc cttcctctgt ctgtccactt gggtcccatg
    76921 aaagatgcaa gacatctttg ctggcaggtt tttctgttag taatggacct ttgacccatt
    76981 gtccttcttt tgtaataaat acgagttgaa cttggtaata attataataa atggcattta
    77041 aaactgaaaa tgcggaaaga atggacactt catgctctgc gctgcgtccg ccatatagta
    77101 aagtaatctt caaaagattg tcctcctgta ttctaaatcg ttcagtgatt tactctcata
    77161 ttttagcaca aaacaaaaat aaacgaataa cttatttata aaaaaacgag ccagcttcta
    77221 aaagaacgag cgtcatttat tttttgtcag ctagattatt tgtcccgttt tttataaaaa
    77281 gttatgataa agaaaaggag ggatgatgat ggcgcaaaca aatccacgca gccgcttggc
    77341 agaaatcatt cgagtgttca tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa
    77401 tcccgaacag gtaaaaaaag cctttgaaga actaggcccc accttcatca agataggaca
    77461 gatgctttct gttcgagaag atttgctttc ttctgccttc actcaaacat tcaaaacatt
    77521 acaagatagt gtacctagcg acacattttc aacggtcaaa aagacgatcg aaacagaact
    77581 ttctctttca ttatcagata tattcgatga tttttcaaaa tcgccttttg cctctgcttc
    77641 tatggggcag gctcatcgtg ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca
    77701 acaccctaac atcgccgaag agatccgact tgatcttcaa ctatttgaac gagcgatccc
    77761 tttgatcaaa tacattcctg aaactagcgt agttgatcta aaaggggtac tccaagaagt
    77821 aaaacgttcc ttgatcaatg aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt
    77881 ctatcaaaag aacaatggct ggaaagagat ccgcagcccc aagatttatg acgctttttg
    77941 ttcgaaaaaa gtgatcgtga tggaagaaat gtctggtaaa aacttgaatc atttaatgaa
    78001 cgccgaaaac aaaactgaaa cttttatcac tggaattcaa aacaagcagc tgaaacaaga
    78061 agttgcaaag cttttagttg agaactttat gaaacaggtg tttgatgatg gttttttcca
    78121 tgctgatcct catccaggga atctgctatt ccatgtacta acaaaagaag aacagacaca
    78181 agctagccga aaaactgaaa cagtccatga aaaagagttc ggttcctttg cttttcgagc
    78241 ctccacttct gcagaagacc ctgttgcgcc atataccatc aactacatcg attttggtat
    78301 gatggggcat ctttctgcag gacttaggca aaaactcaca caagccgttt tagcgctgta
    78361 tacgaaagat gcttatcgaa tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc
    78421 gtttgatgaa agtcggttcc accaagaact gacaagtttt cttgaacagt attatgattc
    78481 tcctattgat gaaatcaact tgcaagaagt attcacacat gtcgtgacga tttgtcatca
    78541 aaataatctg cagttcgatc gtgatatcac gcttttgctg aaagcatttg ggacattaga
    78601 aggggtgatc cgcgtcctcg atcctgaagt ttccctcatg gaagtcgctt cgccttttgc
    78661 tcaacattat tttctcactc atctggatgt agaagataca ttgaaacaaa gcggattaga
    78721 cttattggaa gggatgaagg ctgcgcctaa gatccctcaa cagctccacc atctattaga
    78781 aatgtggact tcgggacaag gaaaagtcaa tttggaattg aaaaagcaag acaaattatt
    78841 gagcaggatc gaaagtatga tcaatcggct tgttttcggt atgattttag ctgcattgat
    78901 cgtagggtcc tccctgcttg tccaagcagc acctgtagaa aacgcagagg tcgtttcctt
    78961 attagggata tttacgtatg ctattgccgc attcgtcatt atttttttgg caatcgatgc
    79021 attgatccaa atgtataaga agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag
    79081 cttcaagaaa taagaagggg ttttacataa attgtttctc aaatttttgt gaaaattcgg
    79141 cttatttttc gaagaggcta cttctgaaac accgagaata aaaaatgtga aaaagctgtt
    79201 ctgcacccag taataagaac aatcaaaaag ggaacatgga acacatttat cctgtttccc
    79261 tgttcccttt ttgatgtatt cgagcataaa taaaatacct tcgttaccta ctatcttctg
    79321 tacttggtaa accttagtca attgcctttt ttaacgcttg acgaaaagac atatgatctt
    79381 tctccgtaaa gggttttgga ccctttgtcc ttatgccagc ttttcttaac tttccactat
    79441 gataacgttg ttctagaagc gtgccgatat tcgcttcgtc aaattctttt ccagagtgat
    79501 ggaacactcg cacacccttc ggcaatacaa gtgaagctaa accataatct tgcgtgatca
    79561 aaaagtcatt cttcttaacc ataccgacga ttttataatc cgcactatca gctccgcggt
    79621 ctacgtagac gatttggaca tgattagcgt attctttggt cgtataatgg tccacactgg
    79681 tcacgataac cacgtcaaga ccataggatt ctcctaattt tatgacatct tcttttactg
    79741 gagaaccatc tccatcaatg aatagcctca ttctttttcg ccttcttcca taaatgataa
    79801 acagttacta atccgctcag taaaagacca acattgatcc attttgccaa gataagcagt
    79861 tgtacacttt ctatcggcac tgattcatgg ttcagcgtga tcaataatag atggattggc
    79921 tctcccattt cttttgctaa aacaaacaaa agcgtcatca ataaaaactg gtggatcaca
    79981 ctccctttat tttgagtagt atacttgaca acgatccggc gatccattgg aatagctgta
    80041 tcagtttgat aatattcctt atttagccag ctgcctgtta ctacgtgcca gaaactaagc
    80101 agaaagacac ctatcgcaaa accatttgga atcaagagga ccaataaaga agaactcagt
    80161 ccaagcaatt gcatagccaa tggagaccat gttaaacgtt tcaaaatcca tggtccgact
    80221 atttttgctg caatcaagcc tgctgcataa ggcaaataca taaaaatcga aagtttttct
    80281 aatccgtaca gtatgccaac ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt
    80341 ccatttagaa aagtaaacag atttagccag cttggcaact tccattcctt cttctggtgt
    80401 cctaaaacaa acacgaacaa gatgaacagc agcaagaaac cataaatagc aagatcaaaa
    80461 agcctttcat cgaataacaa tcgggcattt ctcaaaagaa acaataagcc aaagaatatc
    80521 aaaaataaaa caagctctcg cgtagctacc agatttcttt tgatgtcttt gaaatccagt
    80581 tcgtaacgcg gataatgact aaccgtatgg taggccatga cgtaaaaaag tgtataaata
    80641 gtaaagcttg ctgcaatctg agtcggtgca ggcaactcaa tcgcttgata caggagaaat
    80701 agccagatag ctgcaaataa gtactttttc ccatccatat tgataaaacc ttgcgtcttt
    80761 tcgtgaaaat tgaccgtggt attagcaggt acaagccatg ctgcactcag ccctagcaaa
    80821 acacttgaaa aataaaataa aggaaaataa aatacgccta atattccaag caatgaacca
    80881 gcaccaccaa tcagcagagc gatcatcagc aacgtgtaac tatctagacc aaatcgtaga
    80941 ctgttgacta aaaatagtcc agtcatccga aaggcataaa acagcgtaaa tggcaaaatc
    81001 gtcagccaat tatgcgagtc tgttagtccc aaaaataaaa gataaggaat aaaaataacc
    81061 gtgttactta agataaaggg cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt
    81121 tcgaacatga caatggctcc ttaaacagtc ttataactaa tctgctcttc gattttagca
    81181 ttaaagggga aataaaccaa ttttacagaa tatattcttg taaaaaaaaa tacagaaaga
    81241 agcataagct tctttctgca tttttacggt acttgattac tcaagcagac aagcccttct
    81301 ccacaaaagt cttgcccgac acccctgcat ccggcaggtg ataaagatag aaatcaatta
    81361 tcctgtgcct ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc gacccgtcgc
    81421 ataaatttac tttaacacag ttcctatgtt tatgcgagag cttttttcat tttataatcg
    81481 accatttgaa atccttgacc gatatctgaa ataagttctt ctgtaatctc aaatccttca
    81541 tgcttataaa cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa gatctctctt
    81601 tcctttgttt gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt caatccttga
    81661 ccacgtactt tatttagcaa atataattta cttaaataaa tttttccatt tatctcttca
    81721 taagcagtgt aaccgacagt cttttcttcg ctttttaata aaaagtagga cacaccttct
    81781 gtcagttctt tttgaatctg tttttttgac tgataagttt tcagcatgta atccacttgt
    81841 ttttgaccaa tgatcggcac aaaaacttcc ggccatactt cttgtatgac tgccatcagc
    81901 tcatccagtt cattttctgt tacttttatt ttttccatac acccacgtcc ttttttacat
    81961 tataaatgac cttgcgcttg atggaataaa aagagcatga tcatgctata cacacccacc
    82021 gaccacggtt tcaagaaaat cacaatctgc ataaattccc taaaagataa tttcgtcaaa
    82081 ccagctacta gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt
    82141 acttttttaa tatctttcgt ttttcttgtc agcaattcat caaatttctg atactttttt
    82201 ggcgataaaa taagctggac aaaggctacg ccatagtaac ggacaagcaa aaatccgatt
    82261 gcttccccaa tcactaggcc aatacaacta tataaaagcc caaatccatt gccaaacatc
    82321 agcatgcccg ctactgagga gatccctccc ggcaaaatag gaatgatcac ttggatcact
    82381 tgcaacagaa caaatatcat cactgcatac ttgccaaatt gctggataaa cgcttgcaaa
    82441 gacgtgctcg atcgaaaaat cccatgatga taagcataaa gaatcaacac aaagaaaaaa
    82501 aacagaccaa taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac
    82561 atctatattc cccacatctt ttttcattta ttaaaactgc ctc
//
LOCUS       DANKAY010000003        67559 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000003 DANKAY010000000
VERSION     DANKAY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..475)
                     /locus_tag="PWY98_000183"
     CDS             complement(<1..475)
                     /locus_tag="PWY98_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035972.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQ"
     gene            complement(496..1587)
                     /locus_tag="PWY98_000184"
     CDS             complement(496..1587)
                     /locus_tag="PWY98_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2035973.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(1595..3805)
                     /locus_tag="PWY98_000185"
     CDS             complement(1595..3805)
                     /locus_tag="PWY98_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035974.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(3821..6349)
                     /locus_tag="PWY98_000186"
     CDS             complement(3821..6349)
                     /locus_tag="PWY98_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2035975.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(6401..6811)
                     /locus_tag="PWY98_000187"
     CDS             complement(6401..6811)
                     /locus_tag="PWY98_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035976.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(6824..7042)
                     /locus_tag="PWY98_000188"
     CDS             complement(6824..7042)
                     /locus_tag="PWY98_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035977.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7039..8031)
                     /locus_tag="PWY98_000189"
     CDS             complement(7039..8031)
                     /locus_tag="PWY98_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2035978.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8110..9021)
                     /locus_tag="PWY98_000190"
     CDS             complement(8110..9021)
                     /locus_tag="PWY98_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2035979.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9040..9384)
                     /locus_tag="PWY98_000191"
     CDS             complement(9040..9384)
                     /locus_tag="PWY98_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035980.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(9404..9960)
                     /locus_tag="PWY98_000192"
                     /pseudo
     CDS             complement(9404..9960)
                     /locus_tag="PWY98_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10041..10541)
                     /locus_tag="PWY98_000193"
     CDS             complement(10041..10541)
                     /locus_tag="PWY98_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035981.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(10542..11087)
                     /locus_tag="PWY98_000194"
     CDS             complement(10542..11087)
                     /locus_tag="PWY98_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035982.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11084..12340)
                     /locus_tag="PWY98_000195"
     CDS             complement(11084..12340)
                     /locus_tag="PWY98_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2035983.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(12701..13012)
                     /locus_tag="PWY98_000196"
     CDS             complement(12701..13012)
                     /locus_tag="PWY98_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035984.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13035..14345)
                     /locus_tag="PWY98_000197"
     CDS             complement(13035..14345)
                     /locus_tag="PWY98_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2035985.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(14959..15411)
                     /locus_tag="PWY98_000198"
     CDS             complement(14959..15411)
                     /locus_tag="PWY98_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035986.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(15413..15778)
                     /locus_tag="PWY98_000199"
     CDS             complement(15413..15778)
                     /locus_tag="PWY98_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035987.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(15877..20160)
                     /locus_tag="PWY98_000200"
     CDS             complement(15877..20160)
                     /locus_tag="PWY98_000200"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL2035988.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(20307..20546)
                     /locus_tag="PWY98_000201"
     CDS             complement(20307..20546)
                     /locus_tag="PWY98_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035989.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(20636..20908)
                     /locus_tag="PWY98_000202"
     CDS             complement(20636..20908)
                     /locus_tag="PWY98_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035990.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(20991..21242)
                     /locus_tag="PWY98_000203"
     CDS             complement(20991..21242)
                     /locus_tag="PWY98_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035991.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            21405..22364
                     /locus_tag="PWY98_000204"
     CDS             21405..22364
                     /locus_tag="PWY98_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2035992.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            22534..23214
                     /locus_tag="PWY98_000205"
     CDS             22534..23214
                     /locus_tag="PWY98_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2035993.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            23304..24338
                     /locus_tag="PWY98_000206"
     CDS             23304..24338
                     /locus_tag="PWY98_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2035994.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            24352..24687
                     /locus_tag="PWY98_000207"
     CDS             24352..24687
                     /locus_tag="PWY98_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035995.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            24671..27151
                     /locus_tag="PWY98_000208"
     CDS             24671..27151
                     /locus_tag="PWY98_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2035996.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            27207..28766
                     /locus_tag="PWY98_000209"
     CDS             27207..28766
                     /locus_tag="PWY98_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2035997.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            28763..30127
                     /locus_tag="PWY98_000210"
     CDS             28763..30127
                     /locus_tag="PWY98_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2035998.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            30298..31368
                     /locus_tag="PWY98_000211"
     CDS             30298..31368
                     /locus_tag="PWY98_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2035999.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            31383..32051
                     /locus_tag="PWY98_000212"
     CDS             31383..32051
                     /locus_tag="PWY98_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036000.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            32071..32649
                     /locus_tag="PWY98_000213"
     CDS             32071..32649
                     /locus_tag="PWY98_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2036001.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            33050..33961
                     /locus_tag="PWY98_000214"
     CDS             33050..33961
                     /locus_tag="PWY98_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2036002.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(34339..35256)
                     /locus_tag="PWY98_000215"
     CDS             complement(34339..35256)
                     /locus_tag="PWY98_000215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2036003.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            35458..35895
                     /gene="zapA"
                     /locus_tag="PWY98_000216"
     CDS             35458..35895
                     /gene="zapA"
                     /locus_tag="PWY98_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2036004.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            35953..36501
                     /locus_tag="PWY98_000217"
     CDS             35953..36501
                     /locus_tag="PWY98_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2036005.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            36616..38976
                     /locus_tag="PWY98_000218"
     CDS             36616..38976
                     /locus_tag="PWY98_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2036006.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            39063..39377
                     /gene="trxA"
                     /locus_tag="PWY98_000219"
     CDS             39063..39377
                     /gene="trxA"
                     /locus_tag="PWY98_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2036007.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            39559..41337
                     /gene="uvrC"
                     /locus_tag="PWY98_000220"
     CDS             39559..41337
                     /gene="uvrC"
                     /locus_tag="PWY98_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2036008.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            41482..42381
                     /locus_tag="PWY98_000221"
     CDS             41482..42381
                     /locus_tag="PWY98_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036009.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            42374..43636
                     /locus_tag="PWY98_000222"
     CDS             42374..43636
                     /locus_tag="PWY98_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036010.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            43797..45695
                     /locus_tag="PWY98_000223"
     CDS             43797..45695
                     /locus_tag="PWY98_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2036011.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(46025..46744)
                     /locus_tag="PWY98_000224"
     CDS             complement(46025..46744)
                     /locus_tag="PWY98_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2036012.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            47081..47413
                     /locus_tag="PWY98_000225"
     CDS             47081..47413
                     /locus_tag="PWY98_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036013.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            47400..49403
                     /locus_tag="PWY98_000226"
     CDS             47400..49403
                     /locus_tag="PWY98_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2036014.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            49422..49892
                     /locus_tag="PWY98_000227"
     CDS             49422..49892
                     /locus_tag="PWY98_000227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2036015.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            49913..50497
                     /locus_tag="PWY98_000228"
     CDS             49913..50497
                     /locus_tag="PWY98_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2036016.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            50512..51498
                     /locus_tag="PWY98_000229"
     CDS             50512..51498
                     /locus_tag="PWY98_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2036017.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            51488..51799
                     /locus_tag="PWY98_000230"
     CDS             51488..51799
                     /locus_tag="PWY98_000230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2036018.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            51821..53602
                     /locus_tag="PWY98_000231"
     CDS             51821..53602
                     /locus_tag="PWY98_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2036019.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            53595..54971
                     /locus_tag="PWY98_000232"
     CDS             53595..54971
                     /locus_tag="PWY98_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2036020.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            54978..55613
                     /locus_tag="PWY98_000233"
     CDS             54978..55613
                     /locus_tag="PWY98_000233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2036021.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            55643..56998
                     /locus_tag="PWY98_000234"
     CDS             55643..56998
                     /locus_tag="PWY98_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2036022.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(57190..58584)
                     /locus_tag="PWY98_000235"
     CDS             complement(57190..58584)
                     /locus_tag="PWY98_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2036023.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            58676..59647
                     /locus_tag="PWY98_000236"
     CDS             58676..59647
                     /locus_tag="PWY98_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2036024.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            59644..60426
                     /locus_tag="PWY98_000237"
     CDS             59644..60426
                     /locus_tag="PWY98_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2036025.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(60595..60966)
                     /locus_tag="PWY98_000238"
     CDS             complement(60595..60966)
                     /locus_tag="PWY98_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2036026.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            61100..61543
                     /locus_tag="PWY98_000239"
     CDS             61100..61543
                     /locus_tag="PWY98_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2036027.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(61586..62395)
                     /gene="yidA"
                     /locus_tag="PWY98_000240"
     CDS             complement(61586..62395)
                     /gene="yidA"
                     /locus_tag="PWY98_000240"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2036028.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(62414..63781)
                     /locus_tag="PWY98_000241"
     CDS             complement(62414..63781)
                     /locus_tag="PWY98_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2036029.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(63765..64595)
                     /locus_tag="PWY98_000242"
     CDS             complement(63765..64595)
                     /locus_tag="PWY98_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2036030.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            64758..65195
                     /locus_tag="PWY98_000243"
     CDS             64758..65195
                     /locus_tag="PWY98_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2036031.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            65271..65891
                     /locus_tag="PWY98_000244"
     CDS             65271..65891
                     /locus_tag="PWY98_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2036032.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            66080..66586
                     /locus_tag="PWY98_000245"
     CDS             66080..66586
                     /locus_tag="PWY98_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2036033.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            66603..67502
                     /locus_tag="PWY98_000246"
     CDS             66603..67502
                     /locus_tag="PWY98_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2036034.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
BASE COUNT        21484 a        12571 c        12964 g        20540 t
ORIGIN      
        1 attgaagtaa tacaaattga gtaaccttat tcattgtttc ttctccaaat aacaaatatt
       61 gatccggatt ctctatatct tgtgtcacct ggtaaagttt cccagttgtt ttcatttgta
      121 catgcgggtc tacatcaata ctattgtctt taatacaacg atctgtcata tagtccctca
      181 cagcttgatt gcgtttataa acatctgtga aattactcca ttttctagca aaatctttta
      241 agaaattttt tgtcatttca tctatttgca tttgttgcgc tgtctcgtct tctaatgaac
      301 caatttgact gttactttca gtactttgaa gagttccact tgtttctttt actgcttcga
      361 ttgtttcttc tgtcgttctc aattgtgttt tttgtttttc agaagaaaac caatgacaac
      421 cagtaagacc tatcaaacat aaccaactaa atactaaaat tatccctttt ttcacgtttc
      481 tagtcactgc ctttcttatt tcaccttacg aaccagacta cgaatttttg tttgattgta
      541 cgtgcgttga taccgagcca taatccgtcc ttgctcggca ttctgttcca cactagagaa
      601 atgttgtccg ccatatgaaa catctgttat aattccggtg tgaccataga ttccaggaga
      661 caatgcacca cctgcttgcc aattcaccac atctcctgct tgtaaatcac ttggttttgg
      721 gttcatgatt acttgccaac cataggttgt ccagttataa tcttccccta ttaacatcgc
      781 aaacatgtgt ccagaaccca ttaactgtgg gccacctaat tgagatacat accatgccgt
      841 accaccataa cattccccat tatttacgga tcgtccaatg atccgatcca aaaccttcgc
      901 attgccttta ctttttcctg caataagcga agtttttttt gttggtaggc tattccaatg
      961 cttgatcgca aaagtatcat attccgttaa gttataggac gttatgatac tcattgctcc
     1021 tcgataatac ggactccctt cggtaatacc tgctgccgca tagcctccac gacctagaat
     1081 caataaacaa cttccaggat ctttattgcc cacacattga ttaaagttgc tttggctcgc
     1141 caccataaag ccataatagt cataacaagc ttttaagttc ggaaacttca tatagaaacc
     1201 accttcagaa ccaccaattc cacgtgcaga accttgtggg tagggacaac caggaaacca
     1261 ggtaatccca aaaaagttcg tatcgtttct tccagattgt gactgtccat aagctgattc
     1321 atagcctaat tgagaaagaa tcacactcgg taataactga tactttacag ctgcaccaac
     1381 tgctgcttgc gcatgttcat ctgtgattgg accacgtcct ttaatctctg aaaaaatcgg
     1441 taatccttcg gtatattccc cattaaaagc gacagctaga cctgtagctt ctcctccatg
     1501 ctcagaaagt tctcctgaaa aaaagaatgg caatagaaaa aagggaagaa taaagaaaag
     1561 aagacaacct agacaccctt tcttcatttc ttcctcatcc tttcttcgat aatggttttt
     1621 ggactgttcg tccttttggt aaagtaaaat cagttcctgg ttttgcgtcc aaaatcggca
     1681 gtgttttagg atccttttct attttttccg attgctctgt tggcttatct aaagaagaaa
     1741 atgcaggttg taacgatttt ggtgcggaaa tcgacggttt actttcagac gaacgttgcc
     1801 ccggttgttg cacagaacga tttcctgata gagtggctac cgacgaatta ggaacagagt
     1861 attggttcaa taactgctgc attgctggat aactcccatt tttcgcctga tttttcactt
     1921 ggcgaatctg ttgtgaaaga gaaccagcag ctgttaattg tggcacgggt acttttccag
     1981 aaggcaacgg aatcgattga ccaacaaaag cgataccttt atttaacaat ggttttcttt
     2041 gattattccc ttgttgctta ctcaattgtg ccaatgattg tacctgttgt tttggtctta
     2101 ctgaattcat ggacgaattt ggaatcaaac catttgaaag accagaattc cactctgttt
     2161 tcgatttggt agaagacatt tcaggctcta ccccatgata acgaacatga cgaccactat
     2221 aaggcaaggc ttgatttgaa cgcccaccta gttcaaaggg attaaccact tgcctccctt
     2281 ttaccgattc ttgtggtgta cgtttttgtt gaaacttttg aaatccatgt atcgctgcag
     2341 aagcgagtgg attcgttgct cgatacacat tccctgctaa tcctgcacct ttgattgcca
     2401 aattttgtcc agcagttgat agttgaccag gcagttgttt catttgagac aaagaatgac
     2461 ttgctatgtt tttcgtgttg tttatagttt gatctgcagc tcctaaccct agtttcttta
     2521 ataaatcttt ccgttttaca atgagtaaaa ttgataggag tgctttgaca aacatatttc
     2581 ctaaaaaacc aaaaacagta ttcattggaa ttaaatcgtc tactatgtta tagatcaaaa
     2641 atgccaccaa aataatcaca ctgtaaaaag atttttgaaa catcaagccc aaaaaggact
     2701 ttaaggtatt catgagcata taatcaaata ctggaacaaa agaagcgagt aatgaaaaag
     2761 gaatcacaag taataaacac aacaccaaca tttgaaagat aaaacgaatc atgccaatca
     2821 ctacaagtgg taatccaata cttagattaa gcaatggcac catagaacca acgactgctt
     2881 tataaacagc tttagttggt tgaatataga cacgataagc cttctttttc cctctatctt
     2941 ctcctgcagc agtggtcact tttgtctcaa tatcagataa ttcttcactt gacgcttcct
     3001 tggctagaaa ttcttgtggt tcaatctctg cttgatacaa ttcttctaaa tttgttgtcc
     3061 cataatttaa caatatatac ggatcaacaa cagccgcttt ataataaagt tggcgcactt
     3121 tttgaatggc ttccttttgt gtcgttggca ctaaaacacc attgacatct tttgtcgcca
     3181 attcatctag actatttgta gcagaaaaaa taaagccttc tacctccacg gacaaatcat
     3241 ttaacccttt ggtaaaagct tctcctttac taggtacgtt actaccatag ccaaaccaaa
     3301 ccattgaaaa aatgacaacg aagagaaatc gaataaacac catttttgcg taatgtttcc
     3361 ctctaaatat acgaagagta aatagatata acacatacaa gacgacaatc cccgcaccaa
     3421 taggatcgta tatttttgaa taaatagtat ccgaaaaagt gaaaaactgg tgaattaact
     3481 cgttcattgc agctccttca tagagcttct caatgacaaa atcaaggcct tgccaaatca
     3541 atttacttaa actaaagaac atattcgtca tcatttttag aaaatctgtg ccatttgccc
     3601 caaataacat tttgcctaat gaaaattcac tatcagataa ataagaagaa aaggtatcaa
     3661 tatgaaattt cccaatctca tagatcgatt gatccacctg cttttctgcc gcatacacca
     3721 atccaccaac aaaaagcagt ccgaatccaa tcgttaaaaa aaacaaaacg caccatttct
     3781 tttccttatg ctttatataa gccattgttt cacctcattt ctattgcatg attaacgtat
     3841 cctgttcttc tacttgagag tgacttgttt tttccgttgt ttgtaatgca gcggtcattt
     3901 cttcaaacaa actgtgtaca ttgagttttc ctacacgtcc atagggatct aacatccaac
     3961 actgtccttt aatcatgtgg ctcatttcag ctaagttcgc ttcatttact tccaaacgta
     4021 aatgattcaa aatcttttct cgctcactgt cttcatcaaa agcaaagacg acaccaaaat
     4081 tccctttttc tttatctgaa cctaagtcac caataaattg tgtatctaac actaattgat
     4141 tcatctggga acgccccaca cgtgccattg ctgcaataat actacgtcct gcgttcgaat
     4201 tattaaatac ccatgcttct gtaaaataaa tcgctgtttt cttactacta tctcgttttc
     4261 caaacatttc acaaaaacga cctaaactaa tcatcaaaca caaagacttt cgatccgcat
     4321 ctgtatatag ttctggtcgg acattgtcat ttggtaattt caaatcttgg atctctaaaa
     4381 tcgttgtttt ggcattaaag tctaatcccg ggttttctcc ataagaaaat cctaaacgta
     4441 aaatcgaatc ttctaccgtc aagcgtaaaa actcaccata ttctcgaacc tctttttgtt
     4501 ccatttgttc taaatagtcg accacatgca tcaagccaac ttgttgtccg gcttcttttt
     4561 gaactaaaac ttcttggatt ccctttaata ccgccccttt taaaaggtga ttggtaccta
     4621 acggacttaa ctcatcaatc atatcctggg ctacttgttt cgcatctact cgggataaac
     4681 aaacaattgg atctaataca ccataattac ggggatttgt agcatctaac gtgacataac
     4741 tcattttttg caaatgttct acaaacaaag ggtaatgttt ctgataatac gggtcttgaa
     4801 tgagtttcgt aaaccatttg cgcttttctc ctttgggatc cacaaataaa ctttgtacat
     4861 tcataaacga actatacaaa aagagcaagc caactaaaaa ggtcttccct ttaccggttt
     4921 ttccagttac tgctacatga ggcgcatcaa acaacgcacc tttgattcct tccgcaatgg
     4981 ctagaagatt caaaaagaca aaacgatttg acgcataaat taattcttct aatggtgttt
     5041 tttctcctcg agaaccacca tcccctaatg tatcttgccg accaatatac cagccggatt
     5101 ccatacctaa ttctgatact gctgaaaaca accattcgcc aactgcttct acagttgttc
     5161 ggtgcttcca atgtaactgc cgtcccaatg gttgccctaa taaacattta tagaacaatt
     5221 ctcgttgatc attttttgca cggtggatat ggataatcgt atgaagttgt tgaataaccg
     5281 ttgcactatt ttgacgaact tcttcaaccg tttgaccgta tacaataaaa catgcgatcc
     5341 aatcgacgac aggttgccca ttatctactc gttgctttaa atgttttact aatcctcgaa
     5401 cagttaataa gcgttcacta ggttcttcat cattttgaac caattcctta tgctctgttt
     5461 tgaaccgttc cgatagccaa ttcagtttta aaccaatacc tccctgtcct ttcaagcgtg
     5521 gatagtgaat tttcattcgc aattctgctg ggaaagaaag atcctgagca aattgaaata
     5581 gatcacaatt tctcatattt tcaggtgttt ccccaatagg caacaatgct aaataactac
     5641 tccctaactc atcttctaat tgaagaaggc ctttatgttc cttcggtata cgtacatacg
     5701 ttccttgatt gtctttcagt acttcccaat tattttgttc ttctactttg tgagccattc
     5761 ctcgaatata ttgcagacga ttgagataca acagctcttt ttttactaag ggacgaatag
     5821 aaaagctata aagtaattgt gtcacttctt ttacataagg atcactttca gaaaacaaag
     5881 tttccaaatc atctacttct aatccaaata agcgtgctaa acttcctgct aaatctaacc
     5941 agattagatt tattacatca tgaaaactct ccaccgttgc tttttctttg actttgattc
     6001 ctaaaataaa tgaaggacga tagacttgac cttctcgttc caacaatgtc actgtttctt
     6061 ttgcataata cgcaccaatt gcttgactat cgggagaaaa tccctttcct aatgtgtcaa
     6121 acttcgtccg taaattcatt gttcgtggat acatcaccaa ctccacatct tcataatctg
     6181 ctaaaagcaa cagtaaacga gccatcattt cttttatttc ctgtgtcttt tttggtgtgt
     6241 tttttgaaat tgttcgtgga aaaacatgat agtaggccca gatacaatcc tctccattta
     6301 acaaacaatt tttcttgaat gcttttactg gttcttttac aagcaccaat gaatttcacc
     6361 tcccaataaa aaaggaacaa ccagttgatt gtcccttttt ctaatcctct aaataaacat
     6421 acttattcac aaaaagtaat tcttccaatg cattgtttga taccttgtcc cagttataat
     6481 acacacaatc accattcatc atacgaatca tataacaaaa ccaatcccat aaataaaaat
     6541 gcactttttt tccatcaggt tttagttttg ccagtgctaa cacacataaa taggggagtc
     6601 ctatataaaa aacaactcct gctggtggaa aaaaagaaat gacccaacca ataaatgaac
     6661 gtaaaaataa atattcaaag attacgacca acaaaaaagt tgctgcattg actaatgcaa
     6721 ctggaaatgg aagacgccaa ttgcttgtaa tctgttgtac gcgataaggt tccgcataaa
     6781 caatcgaata atcataaggt tcccaatcca cttcactatc ctcctaatcc ataaataatt
     6841 tttcaatgat tttaccaaac gcatcaaata cacgtgttgg atcatttaag aagaaataaa
     6901 ctgcggcacc tgctgcgata gatattaatg catttcctac ttttgatttc ataaaatttc
     6961 gcaatcccag ataaacaacc accattatta aaaaatagcc accttgcgtt aatataaaat
     7021 ctacaattcc cttcagtgtc atttccgttc ttctcctttc ctatccaacc aattatgtgt
     7081 taatttttca acataatact gatttgtttt tttcgtaatg acaatcgtca tttgttcttt
     7141 ttgtaaaagt gacgtatcac catctcgaaa gacgaccgtt gcttttacaa taaaaggttt
     7201 tttcgtttct ttataacgca atactttcat ctctacttga tcaactttag ctgtttgatt
     7261 gagtccttca ggttctttca tcatgtatga catatctttt gcattgtttt tcgcatactt
     7321 atctaagaaa tcttccacaa atccttcaac atctttcgct tccgacgcat caaccgtttc
     7381 gtataatgtg tcatcataag taatcattcg ttgtttttct gtattctttg gtagtgccgt
     7441 aaaataagga taagcaataa tacgacagcc cttgtttgaa atagaaaagg gaagattgag
     7501 tacttgtgtg attgtcacac ttgtttcttt cttactctct ggattcatcg agcgaatatc
     7561 ataagttact ttataactag cagttggaat ggtttcaatt tcttttaatc caataaacga
     7621 agcagcagtt aattttcgaa cacctgaaac acttcctggt tcttgaataa agttttgtgc
     7681 ataaaactct ttttttaatg tttcttgacg ttgttgttct tgttcatgat tatcgaatgt
     7741 attcatatac aaagcaacaa aacgattcat aaaggattca atttcaggtg tgtacgttaa
     7801 ttctgaccga ctttgtactt ctttcttttg taattgttgg atttcatctt tcaaaaacga
     7861 cacttgaacc ttcgttttca tggattgaaa aaatgccaag ccccctgaaa aacaaagaaa
     7921 taaaaccaaa caccaaatcc atttcactgc tttttttgaa tggattttct tttgcttcgg
     7981 tactttctct gtataaactt tttctttccg ccgtagttgt cgttttttca taagctactc
     8041 cttctagtat gtttttacta gtacgtacga ttgacaacga taagcttgtt tttctgaaaa
     8101 atagtccatt caattaccct cattttctat tccttttctc tttcttttat atccaacaaa
     8161 tccagcaata cttgtcaaca ctcctcctag aacagaaagt gcaacatgta tttcttcacc
     8221 agtctttggt aagacttttg tttttccatc tgctgttttg atttcaatat tgccacttgg
     8281 aagtttcttt acaggaatcg acaaatcctt taccaaattc ccttcactat cttggattaa
     8341 tggaatacca tcttttacac caacaattgt ttgatcacct gctactttta atggggtggt
     8401 tggtgttgga atatgggtca attcctctac attactagtg acaatcggta cactcattcc
     8461 ttgagaagta ttcgtcgtaa tttcaccgct tggggttacc gatactgtga cttcttttgc
     8521 tggaacaata ggtttatttt cttttggaat tgcttctgat ttttcaacag gttgtttcgg
     8581 ttcttctttt tgttcttcac tggtagacga catcgttggc tgttccgtac ttgacgaatc
     8641 agtattcttt tcagaagaac tggttgttcc tgattgttcc ggagttgtga ctatacttga
     8701 atccgttgtt gttgaactat gcacaccttc ttcttcgatc gaatcagtcg gtaaagttga
     8761 ttcaggaaac tctggttctg gtgtcactgg ctctttttca ggtttctctg actgcaattg
     8821 ttcaggttct gctggttttt ccgttgttcc tccatttagc tctgtctcaa catctggtaa
     8881 tccttcccct ggcaatgttt ctacaatttc tgacttaggt gtttctacag tttcatctgc
     8941 ataaaccaat gaactcccta attgaaacga aaataatgaa attcctagtg ttgttacgac
     9001 tgctaatgtt gtttttttca tgattattcc tccaattttt tatgcaattc gtgttttgtt
     9061 catagacgcc gcactaatat tatctgaacg ataaaagaac tcccgtccgt tttcttctaa
     9121 cccaacatat ggattatttt tgaataattg aatcaatagt tcactggtgt tgtattttgg
     9181 atagtttgca aaaaaataat actgtgcttg tcgcccatcc gaaaattcaa caagtaattc
     9241 ataacgatac cgcttcaatc cttttcgttg ttttaaagga tctctatacc aacttgtcca
     9301 tcgttccatt cgtggataga gatagacacg atacacatag aacaatgcca taccacatac
     9361 caccaaaaat aataatcgcc acataaaaat tccccctttt cttctacctt tcttgaatca
     9421 tcgaacattc ataccctcta tagagatttg aaaaatccat catcaattgt tcatataaac
     9481 gttgttgcaa atagcgattg tttcgaatac ataattcttc atagccatca atcacttgat
     9541 cttctttttc atagcatctt gtagctctat gacgctcagc ttgcatgaca taaccaaact
     9601 ctgcaccata tcgttctact gttaataact gttgactacg caataatgca atataacaat
     9661 cacaaaaatg ttcaaccgac atttgatttt tctcacgttt gtgaaatagg tattctttag
     9721 aaagcacatt attcgttaaa caatctgata aatagcgata atctggcggt cggttatttt
     9781 catctgtgaa tttttcccat aataaactga tttttattcg ttcagcggct agatagtctt
     9841 gttcgcgtgg ataaaattca tcaatcgcac aaagtttttt atcacctgaa taaacaaatc
     9901 catataatgc gaaacgaact cttccttttc aattggctct cctaaaaatt ccagtgacat
     9961 accatcttct ccttcctaaa aaaaataaaa aagcagaaac cgatttggct tctacttttc
    10021 tttcctttta tagctctttt ttacggaaga atccgcaaat gacatttttt caaaaaatca
    10081 atcggcacaa gttcacgaaa acttggagca attttggata gaatcacttc cccatcttgt
    10141 aaaatacaat cataaaaatc atcagttact tgatacttac gcttcttaca ataattgata
    10201 aaatagtagc gatcattttc tatttccatt cgttgttcta ctaagcgcat acgaacatca
    10261 tcatagaaat aagccacaaa tcgcccttca agttcatgtt tttcaatggg atactcttct
    10321 ggtaacaatc tattttttcg tcgtttatac attgctggat accttagaag aagttcatct
    10381 aaacgtctgt cagtatatgg taactgacga atcaacgatt ctaatcgttc tttatacaga
    10441 tagaataatt ggcgcaagca atagtgtacc gcctctggat tttctaatac tgttggatca
    10501 cgtgaccaat acgcaccaac ataaatcata atttttttca tttagacaat cacaccagca
    10561 atactaatga ccgatccaca ataggtagaa caagtggcat agcgaccaat ttcctctaat
    10621 tctatctcaa tctcttctaa agaaagatat gtctgttctt ctgttggctg gcgactcatt
    10681 tcccaagtga cagtcaccaa tgaaatttct tccataagag gtttttcgct gctaagtgct
    10741 gccacacagt tttcatatat cttcatttta tcgcagcctt ttttaaccgc taaaatcgca
    10801 ggtattggca atgaaaatcg ataaaagcga atgaattcta gccttccttt ccttcggcta
    10861 aattccaact cttcgagttc ttgttcgtat cccttacgaa cttctaataa gcgtgcaatt
    10921 tcttgccagt tctctttttc ctgataacgt ccagctttta ccgcttgttc aatataagga
    10981 actcgaaaac gaaaataaga caaatcaacc atcttttcat ttaatgcttc gattgcagcc
    11041 tttaaatcca acaggcgcag ccattctgtt gtcgtatatg cattcatgca attttccctc
    11101 ctgacgttcc catccattct agatagtttt gttgctcgtc atttagttcg gcattatcaa
    11161 tcccttcttg tacaaagtct cttcctaaaa tacgatctgc ttcttgtaac aactttaatg
    11221 tcggagctac ttgtttcaaa aaccagcgtt ctgaacgatc taatgagaat ttttctggct
    11281 ttactgtaaa atccaattct tccatcgaac gaaataattt tgcccatcgt ttatcaacaa
    11341 tccgtgcatc actttccagt ctatcccaaa caataagttt tgcattaatt aaaccaacgc
    11401 caatctctcc taaacgtttc ggtgaatcac ctaaaataaa tgcttctact gcttgtgttg
    11461 ctttctcatt ggctaaccgt aactcatatc gattataaat accatatttc gttaatgctt
    11521 ctaattctga aattttttct cggttcgcaa tttcaaaacg tttctcataa aaattaaaaa
    11581 agagaggact ctttcgcgat ccaaaataca aagataatcc ttcacttcga aaatagccat
    11641 catctaaaaa acaaccgcca atattctgga atctcttaaa gtccatttct aataaacctg
    11701 ctttttgttt ttctagtaat tcaaagagat cgaaatgttc ttccgtttcc aatccccaaa
    11761 gttcatctaa cgcaatatcg aatcggctac attgcacacg tgtataacca gcttccaact
    11821 ttccttgatt tttccaaatg gaatatagcc atgttcgcca atctgtttgt tgttcacgca
    11881 tgacttcctc atattcccgg caaccttgtc ctgaaaattg cacaagtaca ttttctgtta
    11941 cagttcgttt tgaatgccag aacacccaga tattcccacg aacagaaaga cagtcaaaaa
    12001 gatacaagcc cgttggacgt gtttcaaatt ctgtcagatc cattcgtaat acacgttgaa
    12061 tcactgtttt gtagtctgtc ccaaaaaagg aaagacgcaa ataatcaatc attgctcgaa
    12121 gatctattcc gcttttcata tacaatcgaa agcgttcctt gattcgttgt tcaaaatcct
    12181 ctgggatttt tcttttccct tgttccatct ttctaatgta atcttttgag cagcccatca
    12241 attgcgcaaa tcttgcttgt gatagcggct ttttatctcc acttatatta tgaattgcac
    12301 ttaacttctt tctgtagctt tttagctcaa tcggtgtcat tttctcgctc cttttataaa
    12361 ataacaaaat cgattttttt taacccttta gaaacaccga tatatcaact agtccccctc
    12421 gttttttggg aaaagtagta caactgctga ccttagataa tcacttgata caaaagggtt
    12481 tataaaactt ttttctatgt gttatgttat ttctgccccc catattatat atcggggggc
    12541 tttacacaaa gaggccgtgc gcaagcgcac gacctctttg tgagctgcac acgctgctag
    12601 cgagtgcgca ttataggccc atgcttcgca gccttaatgc gcaccgccag gcagagtgcg
    12661 gctaccagac tacgtctaga gccctcccga tagataataa ctacaaaatt aaatgatcta
    12721 attttgttat ttcactatat ttttcaggag aaaaaacgcg ataaattgac aaagttaaat
    12781 cctcttctaa atctactcga taatgataca atcgagtcaa tcgttctata tcttttttcg
    12841 ttagcaatac ttgattaata acttttcctg taaaaacacg ttctttgcgc aaaaagcggt
    12901 aatcagttac ttgttcaata cgattcaacc aatcataaaa cgttctggaa gaaagagaag
    12961 gattaatcat tcgtattgct gtagctactc tataataaca actctctgcc atttaacttc
    13021 acctccttag ttgattacaa aatatgcgca tcagaaaaac ttgttccttt tggatctttt
    13081 gctaaataca tttggcgctc tagttgttct tgtaacaact gattatcatc tcccatcgtt
    13141 tgatcttgac tatcctcttt tactgtaaaa tcatagacaa tcatttcagt aaatttctca
    13201 aattctttga taaagtgata atctttgtaa acaaatggcg caaaaaactc tacaggtaca
    13261 tcaaatcctg gaaaagcata tccacgacca ggttgatctt tgttgtaaaa tggcttattt
    13321 ttaaaatctt ctccaaaagt cattaaataa ccaatgggag acaatcgacc aacagaaata
    13381 cggaatgtta aattatcccg aacaccactt ggaaaatatt ctgcatctgg tcgttgcatt
    13441 gctaaaataa tgtacatccc tgcttgacga ccctttaata ctaatggctg aatcaattta
    13501 tcgactctat ttctcgcttc catatccaac gaaccataaa aagctgccca ttcatcaaaa
    13561 aagataaaat gcggtggtaa cccataataa gcataatttt tccctggttc atatttagga
    13621 tctttcatgg ttttcatata ggcataccgt tgattcattt cttttaatgc atttcgtaaa
    13681 caaataatca tttcttcatt tattttgtaa aacacatgtc ctttgaatac atcgacgtca
    13741 cctaatgctg ctaaatctgc cgcttttcca tcgcaaatat ctactgtccc aacacttaaa
    13801 caagcttgaa ttaaagaata caataaatag gttttcccac caccgattgc accagcaatc
    13861 aacatatttg gtgattgatc atatacccaa tccaaagttt tcattaaatg aatacgtcca
    13921 ttttctgcta tacattgatc aataggaatc cgaccagaat ctgaaaggaa tacataattg
    13981 attcgggctt tttctcgttc ttcacgaatt aaatcacgca aaaatacttc agataacaaa
    14041 tgacccattt ttaaaaatcg ttcgtgaaaa cgcccaccat ctaaaggaaa agaaaccact
    14101 aattcttgtt tcttttgata aaaataaact tttggtaatc tcattttttc ccttgtttgt
    14161 ttctcactaa cttgtttctt tgtataaaca aaccgattgt caattaacat tcgaacaagt
    14221 aactgacgcc aataaacaga ggcaaaatat ccttctttta gtcgcgacca atgcatcaac
    14281 cctaaaatta acccactaac cagcaacaaa tacaacacaa tacttattcc aggaataaga
    14341 tacatttgcc aattcgaaaa cgtacttaaa cgtggataaa atactttcca actaaaccaa
    14401 cctaacagaa tcaaaaatgg aaacaaaaag aaaccataat agcaaaacca taaataacga
    14461 ttgaaatagc gaattcgccg accacgatac ttgaaaaaat gagctttcaa ttgtcacatt
    14521 cctcctttct gtttttactt ttcctgtgct aaaaactttt gacaaaaagc aactaataca
    14581 ggaatatcac atttagtaaa aaaacataat cgaaaaagat ttaatcgatt tttataaggg
    14641 tgtgcacaca tattacatat tcgtactata cgaatccaaa tcacttcctg atgatcttgt
    14701 ttccactggt acggtaatga aaaacgaaca agatcacgta aaaacataca ctgcccaata
    14761 accgcacaaa tatatgcatt tgtttgtaca aaaatcgttg gattgtctcc aaaatactca
    14821 atcaattgtt ggatattttg ataacaagca caatctctca aagtatttga ttggttttat
    14881 ctgaattcaa aatctcccct tccttctatc ttagataaaa agaataggaa gactccttcc
    14941 tattctttca aaaaaacctt attttttctc tttttcctta gaatcaacac ttggttgttt
    15001 ttgatctaaa cctttttctt gtgattgttt catattagga ttatttgttg gagcttctac
    15061 tttctctaat cctttcgcat aaatcactaa atctgaatat tcacgattgc catcttgtgc
    15121 tggaatatat accacatgat attctggatc aattaatttc acaggatcac gtcgtttaaa
    15181 accagtcaaa tctacatctg ctggaaattt aactcgtcgt gcaatctttg gttcatctgg
    15241 atttccaaaa actaattgtg ctcgctgtga tcgaatttct cccgtataat cgcctgtttc
    15301 attatcaaat acacgatcag actcttctaa tgcgccaaag cgtacggctc ctaaacaagt
    15361 ttcatctaat gcttctgtaa tgtcataatt atttggaaat tttaatccca tcttacaaaa
    15421 cctccccaat ttttacacca gttaaaaaga caccaacatc ttgatagcca gttgttcgac
    15481 gctctgtttg tcccatatca tttgtttcac gactattcac tcgttcttta cgaaaaacaa
    15541 aataagcttc ttcaaatgaa atacgcacct ctttttctgg taatcgtaat cttttatcct
    15601 cttcagctgt gagataaaca aaaaaagcag gctgttcttc tcgcgaacac tctgcttgta
    15661 attgaacata tcgcccagac acaccattaa tttcctttgg tggattaggt tcccgtgaac
    15721 ccaataaaaa tacttgacct aaaaactctt tcttcatacg caatgctttt tctgccataa
    15781 agtttttcct cctcttaaaa accaaaataa attctaaata tttctacatt acaatcaaac
    15841 aagcggttaa aaatcctaac cgcttgttcc tcatgactaa tctttggctt tacgtacttt
    15901 ataaagtgtt atacctaaag caatgactaa tactactaat ccaagaacca taagtatact
    15961 acttgtttgt tcacctgttt ttggcaacat tggtacaaca gtagctggtt taacccgttg
    16021 ttctggtttc actggttgcg gtggttttgg cgcttccaca atttgaaccg tttgatcttt
    16081 gtttgttaga tctacattgt gtttcgccac ttcttcaaac gtttctttct catcttccgt
    16141 tttcgcttgg agccattccg caaaactcaa gccttttcct gcaagattgc gaccgtccac
    16201 taagaattca aataagaatt ccatcatttc tttatcggcc gcaaagactt tttccgcatg
    16261 agtgccttca aatggtgtgc cgtctggtgt taagtagcct tgaacaatta agcgatagat
    16321 ttgaccaatc gcaagaccgt ctactttgac ccattcaagc acagtttgat ttggcaaggc
    16381 caagatctct tttccgccat ttttattcgt cgctttcgtc gtaatggttg gattactgat
    16441 tttgatggtt tggtcattgt cggtaatatc tgcgtgagaa gccaataata cgccttctct
    16501 aaacagatct tcaaagacca cgacttcttt gcctcgcaac gcacgtgcgt ctagaataaa
    16561 gtctagattg actgtaccat ccgttgtttc tgccacaaac gttaaggtac ttgtgacttc
    16621 ttttccatct actagcactg gtttttccgt tactttgtcc attagaacac cgttgactgt
    16681 gtatgttttt ccaacgacca aatcgtggta tttcacagcg tctgtaatgg tgactttcgt
    16741 taatggatta acctctttca agccagtggg tttatgtgta gccgttgtac gtacacttgg
    16801 gttagttact cgtaccgttt gccctttgtc gttgatgtct ttatggtttg ctagttcttc
    16861 atcatttcta aaaagacttt caaacacgac gatttcacga ccatttatac ctgtcaaatc
    16921 aaaggtaaat ggtacgatca ccgtttcatt ttctgctttg gcaataaagc caacactcgt
    16981 ttctaccgta tttccatgca caagcaaggg ttcaccggtt gctttatcca ttaagatccc
    17041 tttgaccaag tatgcttttc ctggaagaat ctcggtaatt tcaacggtat cgtttaaggt
    17101 aaacaatcct gttgggtctg cgactgtcaa tccgtcttca aagacagccg tggtatgaac
    17161 ggttggatta ttcactttca cactttgggc ttcatcgttg atatccgcat gtgctgcaac
    17221 ttcaatgcca tcacggaaaa gtttttcaaa aacaacgatc gtttgacctt tcagaacact
    17281 agcattaacc acaaattcaa ggtcttttgt gccattgact ttgtctgcaa taaaggccaa
    17341 ggtacttgtg atttctttgc catcttgtaa aattggcgca ttcgttgcct tgttcatcaa
    17401 tactcctgaa atggtgtatt cttttccgac aaacaagttg gtataagaaa cactgtcttt
    17461 aatgacgatc gtttctaatg gatcaaccac tttttcacca tcggctttat ttgacgcttg
    17521 tgtttctagt tttggtggaa tcaagtaatt acgtacagaa accactaacg tttccccatc
    17581 atctggcgca ttttgtttga atgcaaccgt gaatgcttgt ttatctgtat ttggttggaa
    17641 actttctaat ggggcatttt ccaagaagta atagtcatcg gcaactagac ctttcactaa
    17701 aatttccccg ttttgatcag tggtatattc accaattgct tgatttttac cgttatacaa
    17761 ggtaaagttc acacctgcga gcggcgtttt cttcttgttc cattcttcat acttgaataa
    17821 ttttacactt ttccgtgctt gacggttcgc aaaatggatc actttgatcg gttctgtatc
    17881 attggtaatc gagaacacta atgggttttg attcaaaacg taatttacgg gggctttgat
    17941 ttcttccaaa cgatagcgat cttttccata agtaagtgta ccgttagtta ggaaaaagcc
    18001 tttttcattg gttttaaaga tatccgttgt ttctgtatca tttggaatct ccattgtgac
    18061 ataacgattg gctaaagtat caaaaatctt gaattctgta ttagcaagtg gaatcacttt
    18121 tcctgtttct tgatccactt tcaccacttt aagacgttgt tcaattactt tatcttctaa
    18181 aacataatgg aaagtttgcc cattttcaga aattgtgact tcaagtggtt caaatagttt
    18241 atacccttct tttcctttgg tttcagaaag aatgtacgta tcaaagacaa cggtattttt
    18301 accaaaagta gcataaccat cattgtctgt cacaacggtt ttcaccactt tgccagttgt
    18361 ttttgacgtc aaagtgattt caatgccagc taattttggt ttgttatctg gattcgttga
    18421 atcgactaaa tctttattag caactttcac taaatcaatg gttcctttga tcacttgatc
    18481 ttttgcggtc gcattgacag tttttccagg ttcaacaaca atttcaattg cttcatcaga
    18541 aagcacataa ccttttggcg ctttgatttc ttttaaaaca tatttgccag gatcaagatc
    18601 tttaatttct gcctgtactt tgccgttaat ttttttcaag gtaatttctt taatcttttt
    18661 cccatctgct tgatacaaac catagaccgc attttctaaa gaagctgcac cttgtgcttt
    18721 cacaccagtt tccgcatctt ctttaaacac acgaatgttt ccttgtttta gaatttgtaa
    18781 attaataaca gacgcattag gttctgttgg atgtaacaca ccaattgttt gagctcctgg
    18841 acgacgatag gctaatggcg caccttgaac tttaatttta ttcaattgaa tttttgcttt
    18901 atccggtgcc tcttttgtga ctgtaatcca tacttcattg ccgtgaatag atacattgac
    18961 accttttggt ttttgaatgt ctttcacata tttcaaaaaa tcatttttat tctttacttt
    19021 caacgtttcg ccaattttta ccgtatgtgt ttgattatta aaagttgctt tttcaccaaa
    19081 gttattaatc aaacgttgaa tttctgcttt tcgacgatca taatcaacac ggatattttt
    19141 gattgggatt tttgtgccca aagcttccca aatcataaat tgtgccgcaa tgtattgttc
    19201 atcggatttg tctttatttg tgacatatcc taaataagag atcaagcttg tttttttccg
    19261 tacatttgaa tttttcaaat agtttttcaa agcagtagct gtataaactt gaccttctgc
    19321 ggctgagaca ccaggctcaa tacaaaacgc cacttgtcca ttaatctcaa aacgaaccat
    19381 ataatttaaa gtgaagttta gattgttcgg actagaagta acaccaatat tttcgcccca
    19441 atcaattttc cgaccaccta aaacagctgc attggttgca cggcgaacac gaaccgaacg
    19501 attggttggt actgttacta acagtgtagg tgtacctgtt tttggaatgt tctgctcttt
    19561 tttcttttga ttttcaatac tagccgtaga ttctttgttt ggtttcgttg gttgattttg
    19621 actagctgcg gttgaagaga ttgttttttc aacgttttcc tcattttttc cttctttgtc
    19681 agcagtagcc gctggttgtt ccactgtttc ttctattcgt ttcttttctt ccgcttgttt
    19741 ctcagaagac tctggctgca cagaaggcgt tgcttcggca gctttttctc cttctagagt
    19801 ggacgtttct gaagcagggg ttgtaattga ggcagtttct tcaactggtt gtgaagtacc
    19861 aggactattc cctgggtgtt gtgtttcccc aatcgtctga tcgagagtcg ttttggaatt
    19921 gaccgttggc gcttctgtca caggggcctg ttcaacagtg gcttcaccag ctgggacccc
    19981 ggcttctgct ggttggactt ctgtcacttc agaagcgact tgggcaatgg cttccatcgt
    20041 aggttgtata gccgtcgttt cttctgcatg tgcttgagca gttcccacca agaaacagaa
    20101 ttgagaaaaa gccaccaatg cgagtgcttt caaccaattc attttcgttt ttgcatacac
    20161 aagaattcct ccttttcctg agaattttat ataataaaac aaaacgccgt taccaaaagt
    20221 gataacgacg tgaactgtct cttctatttt tatctggcgc tcttatctga acacatttca
    20281 tcaatgattt taccctccca ctataattac agaacaaaaa aagaacctgc ttcgtggaaa
    20341 tcaactgtcc atttcccaat tggaatacca gcttttttgg caacttcacc atcaccaaat
    20401 ctcatgccat accaattact accttcatcc ttaatttctt tccaaccata tgacatagct
    20461 tcatctaatg aatcaaaaat catgacacta ttgccaattg ttacaggatc tggcttaaga
    20521 tcttctggaa gagaaatatc aatcatacca taatgatccg attcctcagt attattgtca
    20581 ttactagcta ctggtttggt tgttctactg gcttattttg atacaacaag tactcttatt
    20641 cttcttttac ttctccgttt tcaatttccc attcaaacca ctccgcaaag tcttgcttaa
    20701 aaccatctac tcgataccta ttttgtaaat aggcatcttc tgacgtcgta ggatctaaat
    20761 aggtaggttt attctctgga cgagcataga cacctaccca tgtttcgtaa tctccttcaa
    20821 aataactatc taccaaatag cctttttttc taagaatctc ccgaacttga tttttaatta
    20881 ttgatacttg ttcaaaacca tcactcattc tatcacctca aataactcta gattctcata
    20941 aatattcccg acaatttcaa tcctatcaat gatctctaac aaatcgctac ttatgcttcc
    21001 ccattcgtat aagaatttcc cttccgcaaa ataaattttc cataaacttc ttcatgatcg
    21061 tcccaaccaa tatcactctc gaaaaattcc acgttgttct tatcttttag ctcagtaaat
    21121 gccataagtt caacatgttt tccaacctcg aaacaatcac accacccatc aaccgttaca
    21181 cgtgtgcctt caatacctaa gaaactttgt cctcgtgtat taaacactaa aactttccgc
    21241 attttttgct cattaattaa tcacgcgcga aatctaggaa tcacaactta ttcgcttctt
    21301 ctttataaat cgccgattgt aaaattaagc tagacaacta aaaaaagtca tttgtgctac
    21361 actcaaaata gttccgacga aagaattata aggagtgagt acaaatgacc tataaccatc
    21421 ttacaccaac tgaactcgta atgatagaag cttatttcaa tcaatcacaa cctgtttcaa
    21481 aagtagccaa tctgttacaa cgttctcgac aaactattta taaggtctat cgttttctta
    21541 aatctggtgg aagcgcaatg agctactata aacaatataa gaaaaacaaa cgtaactgcg
    21601 gaagacatcc gattgtttta cctgatgagc aacaggaata cattcaaaaa aaggttgttc
    21661 aagggtgggc tcctgatgtc cttattggtc gagcagagtt tcctattgct tgttcggtac
    21721 gcacactgta tcgaatgttt aaagataaaa cctttgacac tcacgattta ccaatgaagg
    21781 gaaaaagaaa accaaacggt cataaagaaa aaagaggaaa acaagggttt cgccggtcaa
    21841 tccgagacag aaagacagac tatacccagt ttgatcatga atttggtcac ctggaaggtg
    21901 acactatcgt gggtggaaaa cataaaagcg cggtcatcac attagttgaa cggctatcta
    21961 aagtcatcat taccttgaaa cccgaaggca gacaggctaa agacattgaa aataggctga
    22021 atcaatggtt tcgttgtgtc ccaaaaaact tatttaaatc catcacattt gattgtggaa
    22081 aagaattctc aaattggaaa aagattagta ataccaacga tatttcaatt tattttgcgg
    22141 atccaggaac accttctcaa cgaggattga atgaacattc aaatggctta ttacgtaaag
    22201 atggtttgta taaagctatg gattttaatg gtgtatctga aacctttatc caatcggtgg
    22261 catcgaaacg aaatcatatt cctagaaaat cattacacta tcggacacca atggaagtat
    22321 ttttgagtta cgtcacaaaa gatgatttgt ctaacttaat ttgacaaacg aaacttaata
    22381 aaaaaggaag aatattcatg taaacgattt tattgttttt ttctctttta tacaaaaagg
    22441 gtcataattt caagtaagcg gatgaattca tgaggatttt ttgcatactg tctctactat
    22501 acttaggtca ctaagaaaaa ggagtgattt attatgaaaa agaacatgat tgggacatta
    22561 ttactttcaa cacttcttct aggcggcttg gcaacaccag cttttgctga gggtcaagcg
    22621 acttcaaaag gagatattac ctttactgaa ccaacaaata ccgtggagcc tcttaaccct
    22681 actgatcctt caaaaccagt agagccagca gatccggaaa atccagcaac tggccagaca
    22741 ggtcctttaa cactggatgt agttcccgaa ttgccatttg gtacacacga gattgaaagc
    22801 ggaacaaaaa cgtatcaagt agacgcttca aaaaacgata caccttatct ccaagtatca
    22861 gatcgtcgag gtgtaggtgc agatggccaa gcacaagggt ggaacgtgac agtttcggtt
    22921 tcagattttg taaacggttc acaagttttg caaggcgctg aattagattt tggaacatcc
    22981 actgttaaaa gtacgtcaga taacgaatcc actggaccaa ctagtcaaac tgtaactggg
    23041 ttgagtaaag caagtgcagc gacaccaata tttacagcag caaaagatca aggtctggga
    23101 acatggcttt ctgtttatga tccagcaaat atcacactga aagtgccgaa agcagcagct
    23161 ggaacattca cggctgattt gacttggaac ctcgtggctg gcccagtagc atagagtatg
    23221 aaataaattc aacaaactgc ggtgaaaata ctataggtga aggatttttt accgcagttg
    23281 ttgttgaaag gatgtggtgg acaatgaatc aaagtaaacg actgtttgtt tcgttttttt
    23341 ctattttaag tctatttttc atttttccgt caatcagcca agcaaaggat tcagcaggag
    23401 actttggaat caaaccggta tttccagaga accaaataga taaagcaatc ggttattttg
    23461 atttattagt ggcaccggaa caaaatcaga cactagaagt aattatcagc aattcttcag
    23521 atgaagaacg aacatttgag gtctctgtca atccggcggt aacgagtgat ggtggaacga
    23581 tcgattatag tcaaaaaaac ccaacgttag atgagacatt gccgtttgat gtccgtgacg
    23641 ttttgctcat tgcgaaaaag gaaatcaatg tatcagcaca tgcagaaaca actgtaccta
    23701 tagaagtgaa aatcccagca aaaagcttta agggacgtgt attagcgggt attcatgttt
    23761 cacctaaaga agaagctgaa acagaaaatg ctacagaagg agcacaaatc aaaaatcgta
    23821 ttgcttataa tctcgcagtc gttttacaag aaagtcaaga gacgatagaa ccggatctga
    23881 aacttttatc aggagatttg gacgaggtca acgcaaagcc aactgttcag cttcgtttcc
    23941 aaaatcctca gcctaggatt atcagcaatt tgatatttac gagcaagatc ttttatgaaa
    24001 atcagcttta tattgaaaat acgtctaatg catttttagt tgcaccaaat tcgaattttc
    24061 acttgaatct tgatttagct ggtgataaag caaaagccgg cgattaccgc gcagaaatca
    24121 tagcaaaatc aggcgacagt aatgaatggc gatttacaca aaactttacg atcaaaaaag
    24181 aaaaagcaca aaaagtaaat gaaaattccg tttttgcagt acaagaacaa tctttcccat
    24241 ggctttatgc aggattggtt gctgcagttc tagtgttatg gttgattttg ctgtttttgt
    24301 gggtccacaa gaaaaagaaa aaaggagaaa caacatgatg aggtgatttc gttgaaaaca
    24361 tcgatcttat tgggagggat tattgcaagt ttgctgttct tcagctggca agagccagtc
    24421 caagcagata ccaaaagttc aggtacgatt acattttatg atctaagtcc aattccgcat
    24481 ccaagtggtt cagtcaatgg cgcatcaaaa cagcagacac agctaccgat ttataaagaa
    24541 tctaatcaac atggcgtatt cccggagacg aatgaacaaa agatccgttt tctttatttg
    24601 atcattggta tagaattatg gcttcttgcg tggttgttgt tatggagaag aaaggaaaag
    24661 gaggatgaac atgttgctac aaaataaatt ttcatttttt cgctccttct tttttacttc
    24721 gctttttgtt ttaagtatat ttatcttttc accttcggca gaagctgttg gagcaacgcc
    24781 tcctgccatt tcttatgatc catacacgta tgaaaatatc gtacctccaa atggtggacg
    24841 tagtgcagtc aatggcggga agcttgtcca actaactggg gctaaccaat tgcctaaatt
    24901 gactgctact tatttgaacg atggttcaaa tgatccttgg aaaggtggca cggtccgttt
    24961 tcattgggaa agccatgtga aactaacaaa tgtacttgat ttggtgaata tcactgcaca
    25021 aggatttgca tttgtggcga tcatcaattt gccggaacat gtatcaagca gtgatgtatt
    25081 aaaagcaatt gactggaaca acgcctactt gtatattgcc ggtgccccca tcaaggttac
    25141 tagcggaagt ttattatcag caggaagcca taccttgcgt tttgggatgg ggtcgtggga
    25201 tacaaatact ctattgacag atattattgc tattattacg cgaggcgaat tcaacgtgga
    25261 cgatatcccg cttcaatttg atgttacagc taatgtcgcg aagatgacag aaaacggaga
    25321 taaatatgat acgagtccaa acaaaatttt gaccaagggt aaattacaac cttctatgtc
    25381 taaaaaggcc gttttttctg ttgattttta tgattcgaat aatattatcc aagatcgtat
    25441 gttcaattcg attttagctc cagctgcagg tagattttat cctaaattaa ctggcggaaa
    25501 cttgaatcca ggagcatatg cattttctgc aagtgcagag ataaatacgt ggaatagtta
    25561 catctcacca agtgattcaa caggaaaata taatacattt gctgatacca cagaatacgt
    25621 ggatggaaat aaccagaacc ttccaggctc taccaatagt cggacattga cgatggggct
    25681 gactgaagga cgttttgatc agttgccatt tagtcgcttt gatcgcgtag tcaactattt
    25741 tacaggaaaa aacgttacac aaggagcaat actgactcat actccagcaa gcacaagtgg
    25801 attgaatcag acagccaata tcgtgtacag tgggaaagat gcgcaaggcg gattgctttc
    25861 tcctgttgct ttaaaggtat tggagcagta caagctagac ggaacggttt taccaacaaa
    25921 cttaacagcc gatagtcagt ggggaaagct cctttcttct gcatcgtacc aagtcacggc
    25981 caattggtca gcaactgaac taaaaaaggg ttcgatccat tacaggcttt attcgaaagg
    26041 aacccaaaaa ttagcgacag gatggacaga tcaacttttc caaacgatcc aatcaaataa
    26101 cgggggtgta aatacagcta cttctacact acctgcattg gcttcagggc aatattattt
    26161 tgattatcgc ttggtggacg atgtacttac taacatgtat ccgtcttttg cgtacaaatg
    26221 gcagtctgaa caagctggaa tcaccactct tcctcaaata acagtggcta attttccatc
    26281 tgtgagtgca gatagtcagc taaaaaacct tagcagacaa cctgagacag gagatcccct
    26341 gtctgctcta tcaggagata cgattcaaga aaatgtgaca ttcacgttaa caaaatctgg
    26401 ggatgcgata accgacaaaa aaatcacgat ctcacttcct gaaaatacta cttatatcaa
    26461 tggttcctta aaattaaatg gtacaactat cgcagatacc ggcattcaac aaggagtttc
    26521 tgtcccagca gatctattga gcaaaatagg agataccatt catcttactt acaactatca
    26581 attaaatact gtagatactt ctttacaatc cgtcagcata ctgacaaagg cagctgtact
    26641 ttccagcaat attacattag cagatggtgc taagcttcct aacccagttg tccaaacatc
    26701 tgctaaaaca attcttgtcc ccaaacaaga attaacatta gttaatgtac cagatgattt
    26761 tacatttggt aacgatctcc ccaaaccgtt gaagacaagt tattatgaat caaaaggcga
    26821 tttttctttt gatgtgcggg atactcggct tccaagcacg tctccttggc aactgacagg
    26881 tacattgact tctttattta agaatgatca aggtcaagaa ttaagcggaa caaaattata
    26941 cttcaatcat tctggatcaa agcaattgat tcagcaaggt caaaacacgt tgatttatga
    27001 gagtgatgga acagctaaag gagaggttct cgttgacttt cccgataccg atggtttact
    27061 cttagaggta aatagcagta cgaatgctca acctggcgca acgtatcaag gcatggtcac
    27121 atgggaactg acagctgggc caaccagcta agtgattaga atttgtatgg atctgtgata
    27181 actgtcacag atcctctttt tcaaggatga gaaggaaact tctgctgtta aaatcagaaa
    27241 tgcttgaagg agtggacttg gaacttttca gtttgcttga aaaaaatgaa caacggcaaa
    27301 tcaaactttt ccaactttta ttagaggaaa aaggagaggt ttccgttaat aacgtactta
    27361 cccgcttatc agttgatcgg gctaccttga aagaagatct gttttattta caagctaact
    27421 tggaaaatat caaacaattg gaaattgtta tccaagaagg gaatatttta ttaatcagac
    27481 atggaaatat agcagcagtc gatgtttatt atcattgtta cttgcgctat tctacaaaat
    27541 atcagatact tctttatctg ttggaaaatg gctcatttga tcggcagaaa cttgtcatgg
    27601 aacttaatat cagttcggct actttgtctc gcagagtgaa ggaattaaat gattctttaa
    27661 atgagtttca tctgaaaata aaaagcgggc ggctgaccgg tccagaaagt caaattagtt
    27721 atttttattt ccaattattt tggtttggac gtccctatct tgaaaacaaa aaagaataca
    27781 gtgatttatc aggaaacact tttttagatc tgctgcaaaa agagtttagt tttgctatta
    27841 cagaagaagg acgattgaaa tttgaaatat ggctttccat tatgaagaag cgaatgcgct
    27901 gtacgaaaaa atccaaacca gattatagca atcttcctgt tggattgttt caaacgacac
    27961 aatttttatt ggacctccat acatttttga cccgttactt ctttcaatat gcctttcctt
    28021 ggaatcaaaa tgaaacgttg gttttttatc ttttcatgat cagtaacttt acattagata
    28081 cacataactc gcatgtccag gagtttttga taagcgaaaa atacaaagac gacgtagtag
    28141 ctcaattgaa tcgaacgttt aaacaatcga tagaagaata tttctctttt tatgtttttc
    28201 cagaagagat ttcgcaaaag attgcgttga cagtgatgca aaatcacgct cattttgcct
    28261 attttagagg atggatgaat atttttggcc aagaaggatt aaaacagcgg ttaactgaag
    28321 tagaagataa acaattcctg gttttatgtg aaagattggt cagacaaaca tttgctattg
    28381 ttgattttac tgaagtagac gaggaaaatg cccgctttga attatttgct cgctatgcat
    28441 ctattttacg gacgctgatc aagcagttag aattacgtct gcaaattgct tatgatcttc
    28501 cttatgaaag gctaatggcg gatatcgtga aagaaatcat ccaggaacaa ttgctgaata
    28561 cgatcaaata tgatttagtc gaatacgaga aagaaaagca gtatgatatc attttgacta
    28621 gccaattgaa agagtacccg tcacagaagt ccgctaaagt ttttgtattc actagcaacg
    28681 aatataatta tgatttcccc tatttgaacc aatttttaaa agaatgttat ttagaaaaat
    28741 tgaatttaag gatgaataaa acatgaaaat cgtgatcatt ggtgcttccc atgaaggcat
    28801 cacagctgcc ctaaatctaa gaaaacttca accgaaagca gaagttttac tgatcgatga
    28861 agatcataaa gatggactag gctatgtttc aaatggaatc aacctttatt taaaaggaaa
    28921 gatacgtagt ttagctgaag ttgcccataa catgcggact cttcaagaaa gtggggcaaa
    28981 actgatcacc gaatggagag taactgaact tgatccagac aaacatcagc tcatcctcgc
    29041 atccaaagaa ggaaaaaacg agaccataac ctatgataaa ttaatcgtag cgacaggttc
    29101 ctctcctgtc accttgtata aacaaattga agctgaaaat gtctacacct ataaaaatct
    29161 agtgcaatct aagcaagtac ttgccgcttt aaaagaagca aaagaagtag tgatcttcgg
    29221 tgctggttac attggattgg aattagcaga tgctttgaga aataaaggat acattattca
    29281 tctagttgat tatatgccga atgttttatc aagatatttc gataaggata tgatcaacag
    29341 ttttcaaaat cagctgcaaa ctaagcaaat aaatttttat ccaaatgaat tcttgatcga
    29401 ttggaaaaaa tcagaagaga aagtagtatc cgtacaactt ctgtctcaaa caataaaggc
    29461 agatatggtc attttttctg cccaaactcg tcctaatacg acattgttaa aagaaaaagt
    29521 ggctttgtat gaggatgaga ctgtgatagt caatgaatat ctgcagacaa gtgatccgga
    29581 catctatgcg attggagata tcgtcccagt cagttttgac aaaaataaac ggcatctctt
    29641 tttaccttta gttactcgag ctgtacatat ggcaagagca gtagcgctaa ctttgagtgg
    29701 tcagccaacc gcttacgacc tcagacaaaa gataacagca gcagtcatta tagactactt
    29761 tttaggaact gtagggctga cagaagatga agcgccattc ttggaacaaa ataccggttc
    29821 gtgttcagga gaatttgatc tatttccaca atacgacgaa gaaaacaaaa cagtaaatgc
    29881 aaagctgatc taccatccgg atacattaga aataattggc ggacagctga taagccaaga
    29941 attcttatta tcagatttga acctgcttgc ggatatcgtg aaacacaaaa ctacgattcc
    30001 acagctagca gttgaggatt ttggatttct gtctgaatac acctctcgtt ttcactacct
    30061 caatgagtta gcttttaaag tactagctaa gagagcaaat agcaatagag tagataaaag
    30121 actgtgagaa atatcacggt cttttattgt gtggggatat aataaatatt aatcaatcga
    30181 gaataaaaaa ttcatataat gaaatagcgg aagaattttt tgagcataat attcgccaaa
    30241 aatttatcaa gtgataaaat tgttgtataa aatgattatt tcaggaggag gagttaaatg
    30301 tttttagcat taaatgaaat tatgcattca aagttacgtt atgcgttagt tgcaggggtg
    30361 atgtttttga tcgcctacct ggtatttttt ttgactgggt tagcttatgg cttagctcaa
    30421 gataatcgaa cagcagtcga caagtgggag gcagattcga ttgtgctttc gaaagatgca
    30481 aattcaaatc tagggatgtc catgattact aagaagatag cagaagaagt cgaaggtggg
    30541 aaagtggcgt atcttgccca aacacctgga gtcgtgacta gtaaagacag tacagaagaa
    30601 ggaaaaatca atgttagctt cttcggtatc gataaaaatc aattcatcat gcccaatctt
    30661 gttgaaggaa aagcttttga taacgatgat gaggcagtag gcgatattag tttgaaagaa
    30721 gaatatggtc tagccatagg agatacagtt aagttatctg gtagtgacaa gacgtttaaa
    30781 ctaactggct ttacggatca tgccaaattc aatgtctcac cagttctcta tacaaccatc
    30841 aatgcttacc aagaaatccg cttcgaaaaa gaagatacaa gtgaaaatgc acggattaac
    30901 gcaatagttg tcagaggtaa aatcagtaat ttacctgaag atttagaaca gatcaaaatt
    30961 tctaaattca tcaatgaatt acctggatat aatgcgcaag tattgacttt tggatttatg
    31021 atcggatttt tgatcgttat tgcggctatt gtgatcggga ttttcattta tgtactgacg
    31081 atgcaaaaga ttaatatctt tggtgtaatg aaagcacaag ggatcacagg aggatttatt
    31141 gcgcgttctg tagtggcaca aacattcatt ttatcatttg ttggtattct tcttggtcta
    31201 ttaggaacag tgggaacatc ccttgttcta cctgatgcgg ttccattcca aagcaattgg
    31261 ctgttttttg gcgtcattag cttgttgatg ttagtagtag ctgtgttagg cgcactattt
    31321 tctgttcgga caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga aggaggaaga
    31381 acatgaaagc aatagaattt aaagaagtag aaaaaagttt ccaagatggc gaccaagtag
    31441 ttaaagcatt gaaagaaaca accatgagta tcgataaagg ggaatttgtt gcaatcatcg
    31501 gtccttctgg ttcaggtaaa agtacttttt tgactttggc aggcggattg cagacgccta
    31561 gcagtggctc tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa agcgaacgcg
    31621 ttaaactacg ttttaaagaa attggcttta ttttgcaagc ttccaatctg gttccttttc
    31681 tgacagtaaa aaagcagtta ttattagtag ataagattaa acatgaacat cgtcaaaaag
    31741 aagcaaacga attatttgag cagcttggcg tggataagtt gatcaacaag tatccagaag
    31801 aactatctgg tggagaacgg caaagggtcg caatcgctcg tgcattgtac aatgatccgt
    31861 ccattattct agcagatgaa ccaactgcta gtttggattc agaaaaggca cgagaagtag
    31921 tcgatatctt ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg gttactcacg
    31981 atacacggct gattgacaaa tgcgatagaa tatttatgat ggaagatggt gtattgaaag
    32041 aaaaagaata accaaaaagc aggtggaaaa atggtccatc gatacaacaa agatacacag
    32101 caaaaaattt tgaaagaagc gactgacttg tttatgtcca agggatatct cggcacttca
    32161 acaagagaaa tcgcacaaaa agttgggatc acccagccca atctctatca ttatttcggt
    32221 gataaagaaa aactatatac ggcagtttta gaaaaccact taaaagatgt tggaaaagct
    32281 ttgagagaaa tcgttcaaac aagcgaaacg ggatttcaag taaccttgac aaaaatggcg
    32341 cagtttttga ttgagacaca tctggtcgat ttgtttctga tgcttcacga tcttgaaagc
    32401 aatctttcaa aggaaacaag agatcatctt ttctttctat ggaagaaaaa ttatcgtgaa
    32461 ccttttgaag aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat ttcaaaagaa
    32521 atcgcagcaa ggcatttttt cttagtgtta gcaccttaca tcactcggtc gaaaagacct
    32581 gtggaagaac cgctaaatgt ggcgcaattg atcgatttat atttgaatgg tgtattagga
    32641 gaagcgtaaa gctgttatat agggagaaaa tagttaggtg aagaagcaag atatgacgaa
    32701 gatcgttata tcttgcttct ttatataggt agtttttcat aaatacaaat ttgttattct
    32761 ctcatgatta tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat
    32821 ttaataacga agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa
    32881 tcaccacgaa tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta
    32941 gcagtaatgt tattttctac ggttatatct tttacagccc cttttttgct tacttaagct
    33001 gattttaagt tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat
    33061 aattggagct tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt
    33121 tggtcaaaaa aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac
    33181 aaaggaatca tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg
    33241 tacactatat acagatgact cgctaactac cccacttgca actataaacg gcggagagct
    33301 ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac
    33361 tggatattta gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga
    33421 agagctatct gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc
    33481 attaagcaat gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt
    33541 aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt
    33601 agtacagctt ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag
    33661 tttacacgca gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta
    33721 ctatggacaa gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct
    33781 ggattcaaga ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc
    33841 tattttaatc gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga
    33901 cagttatcag cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta
    33961 ataaataagc ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt
    34021 atcgcagatg caagctttta aacaccgttt aaggaagagg tcgtgagcct gccgttcaac
    34081 tctgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact
    34141 tgtgaccgag gagttggcat gagaacaccg tttaagaaaa aggttgtgaa atcaacgttt
    34201 tgttctgagt attagagaaa caaacggaaa aatagttcct catattttgc cgtttgttga
    34261 gctaataccg aggatcagat gattgaacac cgtttattcg gaacaaggga ctgtgacatg
    34321 acttttgtca cagttccatt atttttttag tagcttcaaa gctttttctg tattggcaaa
    34381 atgaagtttg gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc
    34441 tacttggtca gatttcgttc cagctcctga cggaagggtg attccagctt cagctgatgc
    34501 tttgtctaac tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa
    34561 atgatattgt tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa
    34621 acgcgcatat tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg
    34681 tttttctggc tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt
    34741 catccgaacg gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag
    34801 cagtttatag gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt
    34861 taactctttt gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac
    34921 ataagcagca catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc
    34981 taaaacggat aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc
    35041 ttgtttatcc tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa
    35101 catcactttt ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct
    35161 agtataagga atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag
    35221 ttggtcgttt gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat
    35281 aaaactatac cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt
    35341 tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag
    35401 aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg
    35461 gcgcacgaaa aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga
    35521 cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa
    35581 ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat
    35641 gcgctttcgg atcaattgaa gaaacaagaa agaatattgg agctggaaga agaaacagca
    35701 gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa
    35761 gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt
    35821 aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa
    35881 aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga
    35941 aaggaacgta gcatgctgag tttgctgatt ttatttttat tgctaattgc gtttttttct
    36001 ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga ttggatatgt attgtcattt
    36061 attgctgccc agcattttta caaaccatta gcaagtcatt tggaattata cattccatac
    36121 ccagctgtca ccccaacatc aaagttagca ttttttgatc aggtatttgc ctttcaccta
    36181 gatgaagctt tttatgcagg aactgctttt ctgattattt tgctaattgg ctggctgctt
    36241 acccgatttg tgggggtttt tgtccatgga ttgacgtatg tgccgatctt gagacaggta
    36301 gactggattg caggaggcat cctgtcacta atcatggctt acgttactat atttttgatt
    36361 ttgcagttac ttgcctttgt tccattagat gtggtccaaa atcaattcgg cgagcatagt
    36421 ttcgctcgtt ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg
    36481 gtaacgaata ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga
    36541 gtagtttatt taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa
    36601 agataaggtg ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa
    36661 cagatggtca gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta
    36721 ccagtgagtg agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa
    36781 gtgcaacgtc tgcgaggcgg gatccctata cctaaaattg aaaatatccg tccccatatg
    36841 aaacggattg agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta
    36901 ttgtctacga caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa
    36961 tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt
    37021 ttaaaggaag caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag
    37081 agcataagac agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc
    37141 gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc
    37201 tacgttatcc cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa
    37261 agtgcttcag gtcagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt
    37321 ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct
    37381 gctgaacttg ttccccatcg tcaagaaatc atccacaata cttatgtgat tggtaaaatg
    37441 gatctgatga atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc
    37501 agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa
    37561 gtagtttcta atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct
    37621 aacacaggtg ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa
    37681 gcaggattac cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt
    37741 gcggatatag gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg
    37801 acgaacactg tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag
    37861 ctcggtgcag gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat
    37921 cttggtaaaa agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat
    37981 ggatataatc gggcaaatac gattaatgcc agcatggagt ttgatgtgga tacattaagt
    38041 ccaacgtatc gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc
    38101 cgattaggtc ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc
    38161 caagatttaa acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa
    38221 tatctggaaa tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa
    38281 gcttacagct atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca
    38341 aatgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa
    38401 atgcaacagc agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc
    38461 caattagcaa atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg
    38521 aaagaacaaa aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga
    38581 gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg
    38641 aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt
    38701 atccatgctg tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga
    38761 aaacgatacg aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct
    38821 ggttatcctc aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt
    38881 actgattatc ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa
    38941 ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg
    39001 ttcgctatcc aagtatagaa atgctatact agtcatagat tttaaaaatg gaggtctttg
    39061 atatgtcaca agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat
    39121 tgattgattt ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc
    39181 agttggaaca agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa
    39241 atccagaaac tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag
    39301 atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga
    39361 tcgatcaata tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt
    39421 tccgtcctcg gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat
    39481 aattacgttg tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa
    39541 tatgaagggg agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat
    39601 cagcccggct gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc
    39661 aaaatattaa aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa
    39721 cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca
    39781 ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag
    39841 gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt
    39901 actcgtaagg tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg
    39961 gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt
    40021 cagacgaagg agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc
    40081 gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga
    40141 ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg
    40201 gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact
    40261 tgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat
    40321 aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat
    40381 gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag
    40441 ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac
    40501 aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag
    40561 aaaaaattag ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat
    40621 ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg
    40681 aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat
    40741 cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa
    40801 tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat
    40861 cgcagatatt cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat
    40921 ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat
    40981 attcctattg ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt
    41041 acagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga
    41101 atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac
    41161 agttttgctt cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg
    41221 cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa
    41281 atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga
    41341 aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt
    41401 ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt
    41461 tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac
    41521 tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt
    41581 ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa
    41641 gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc
    41701 ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt
    41761 tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg
    41821 atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga
    41881 aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg
    41941 gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg
    42001 gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa
    42061 aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta
    42121 catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa
    42181 caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta
    42241 gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc
    42301 ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa
    42361 gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt
    42421 taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta
    42481 tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga
    42541 taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc
    42601 agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt
    42661 agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg
    42721 acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc
    42781 gagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac
    42841 tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac
    42901 agtccaatac gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta
    42961 tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat
    43021 cctgtccagt acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt
    43081 tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa
    43141 agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc
    43201 attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc
    43261 agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt
    43321 gacttatcta tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa
    43381 caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat
    43441 tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt
    43501 agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt
    43561 ttatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga
    43621 acgaaaaaaa gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc
    43681 agcttctttt tgtatagacc tattttattt attttactat attggatata tattcattga
    43741 aaatagtgta taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg
    43801 accgttattt agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa
    43861 tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata
    43921 tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag
    43981 aaaagatcaa agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta
    44041 tttttacggc ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat
    44101 cgcagctgat ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt
    44161 caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata
    44221 tcgatgaccg tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt
    44281 tgatgaagga agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca
    44341 tcgatcattt gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga
    44401 tggatcgaat catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt
    44461 ggattggtgc gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac
    44521 gctataatta tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt
    44581 cttttgctca tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc
    44641 tatccgaaag agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat
    44701 tcaaatcaga acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat
    44761 tgctggaaaa aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt
    44821 tgaaaggaac ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg
    44881 aacaaaaagt gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac
    44941 atatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt
    45001 ttcatggctg tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat
    45061 cttatcgcgg aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag
    45121 atctgactgc aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat
    45181 tttcgccttt attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc
    45241 ctcatacgca cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca
    45301 gtgaaaaaat tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc
    45361 acacgccagt aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg
    45421 tcattgacgg aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac
    45481 tattatgcaa ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac
    45541 aacaatttgc aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac
    45601 cacgcaaact caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc
    45661 tcaaaaggct gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc
    45721 atagactgga gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt
    45781 cttctgccaa ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt
    45841 gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg
    45901 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt
    45961 ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag
    46021 tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt
    46081 gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa
    46141 ccgcttcaac gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt
    46201 tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca
    46261 aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt
    46321 catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa
    46381 cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg
    46441 cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg
    46501 gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt
    46561 tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag
    46621 gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat
    46681 ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt
    46741 tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta
    46801 tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt
    46861 tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa
    46921 gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg
    46981 acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg
    47041 aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc
    47101 tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca
    47161 gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta
    47221 tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga
    47281 aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca
    47341 atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta
    47401 tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga
    47461 ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa
    47521 caaacaacca gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa
    47581 aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac
    47641 accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag
    47701 atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt
    47761 taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat
    47821 ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca
    47881 tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta
    47941 agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt
    48001 atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca
    48061 ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc
    48121 aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg
    48181 gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa
    48241 aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg
    48301 cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat
    48361 acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga
    48421 aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat
    48481 atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc ttcgggatga
    48541 tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac
    48601 tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac
    48661 catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac
    48721 tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt
    48781 tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa
    48841 aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag
    48901 ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat
    48961 ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga
    49021 ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac
    49081 tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag
    49141 gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat
    49201 tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc
    49261 taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata
    49321 caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc
    49381 acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat tacttaatta
    49441 gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag
    49501 ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa
    49561 gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt
    49621 tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg
    49681 tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag
    49741 gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac
    49801 atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg
    49861 ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc
    49921 cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga
    49981 acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac
    50041 agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct
    50101 aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt
    50161 aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat
    50221 ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc
    50281 tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact
    50341 tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa
    50401 cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac
    50461 gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac
    50521 cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt
    50581 gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt
    50641 tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag
    50701 cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc
    50761 tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat
    50821 caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat
    50881 gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa
    50941 gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga
    51001 cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca
    51061 gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag
    51121 aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa
    51181 gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct attagagaca
    51241 gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt
    51301 gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc
    51361 ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt
    51421 ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga
    51481 caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa
    51541 attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga
    51601 agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag
    51661 cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa
    51721 tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt
    51781 aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat
    51841 aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat
    51901 gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc
    51961 gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac
    52021 tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat
    52081 ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt
    52141 tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg
    52201 agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca
    52261 taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt
    52321 cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat
    52381 gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc
    52441 acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc
    52501 agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa
    52561 atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac
    52621 ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga
    52681 acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat
    52741 ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat
    52801 ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga
    52861 aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga
    52921 acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat
    52981 cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg
    53041 ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc
    53101 gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca
    53161 tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga
    53221 acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg
    53281 tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga
    53341 tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac
    53401 ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga
    53461 cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt
    53521 agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga
    53581 aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga
    53641 tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa
    53701 atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa
    53761 tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc
    53821 ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac
    53881 cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa
    53941 tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg
    54001 atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc
    54061 ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg
    54121 atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg
    54181 aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg
    54241 accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg
    54301 cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg
    54361 cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt
    54421 atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag
    54481 gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc
    54541 ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga
    54601 gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa
    54661 ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct
    54721 atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg
    54781 atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag
    54841 gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga
    54901 tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag
    54961 aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc
    55021 gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag
    55081 atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa
    55141 tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg
    55201 aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag
    55261 acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga
    55321 cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg
    55381 atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc
    55441 aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga
    55501 cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc
    55561 gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat
    55621 ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc
    55681 ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact
    55741 taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac
    55801 tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa
    55861 cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat
    55921 ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat
    55981 attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat
    56041 cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt
    56101 gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt
    56161 ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta
    56221 tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg
    56281 tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac
    56341 cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg
    56401 ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt
    56461 gacacctaga acggctggat attattcagt tgattacctc catatgagcc atgcaggtct
    56521 gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt
    56581 gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac
    56641 gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt
    56701 attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac
    56761 gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac
    56821 agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat
    56881 agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa
    56941 agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt
    57001 tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
    57061 cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg
    57121 aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt
    57181 atcaataggt tataattcat atttttttat tttttctttt acgatactta gtccagaagc
    57241 taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg
    57301 tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat
    57361 tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt
    57421 tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa
    57481 tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac
    57541 atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac
    57601 atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc
    57661 agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg
    57721 atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc
    57781 aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt
    57841 acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat
    57901 atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg
    57961 agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga
    58021 gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga
    58081 ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa
    58141 atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt
    58201 tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa
    58261 atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg
    58321 agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt
    58381 ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga
    58441 gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat
    58501 caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta
    58561 attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac catacaattt
    58621 taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa
    58681 aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat
    58741 gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga
    58801 aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga
    58861 gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt
    58921 accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt
    58981 gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga
    59041 ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt
    59101 gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat
    59161 tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac
    59221 gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt
    59281 gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa
    59341 tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc
    59401 tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc
    59461 catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc
    59521 tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct
    59581 agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt
    59641 aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat
    59701 ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc
    59761 attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt
    59821 agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca
    59881 tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc
    59941 ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg
    60001 ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa
    60061 gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg
    60121 cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca
    60181 gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa
    60241 gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat
    60301 aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta
    60361 aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt
    60421 atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac cttatagcta
    60481 caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt
    60541 ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata ctaactatcc
    60601 tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt
    60661 gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt gctcgtgtaa
    60721 tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta gatcatacgc
    60781 accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa gtgtcaaata
    60841 aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc
    60901 cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg tgtgagccat
    60961 tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga agtaaggaaa
    61021 tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga aaataaaact
    61081 gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc aatttgctga
    61141 ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga ttgcagtaat
    61201 cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag aagagacgaa
    61261 agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag aaatttcttc
    61321 ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt attattataa
    61381 tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac
    61441 caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac gtggtagcca
    61501 ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc
    61561 agctttctct cagttttttt atttattaca gtacatattt tttgattgct tcagctacac
    61621 cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga gaaacggcat
    61681 ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc tcgttatctc
    61741 cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc gcaactgccg
    61801 cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt ttgaccgttg
    61861 tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt
    61921 cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca ggtgttcgat
    61981 attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg atatctcgat
    62041 taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca cggcgtgcca
    62101 ttacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg attttacctg
    62161 atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta tcttgaaaaa
    62221 ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg cataaaacga
    62281 tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt gttacttctc
    62341 ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata gacataattt
    62401 cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg ctggcaaatt
    62461 cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca accatttctt
    62521 ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca acaagctgac
    62581 taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat
    62641 gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa ttcacagcag
    62701 tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct tggactaatt
    62761 ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc aaaaaacgtt
    62821 tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag taagtgccca
    62881 agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca agaaatggca
    62941 gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc
    63001 gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg aagtacactt
    63061 gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg ccactcatgg
    63121 aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta agatcaaatg
    63181 ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt cgatctgcat
    63241 cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt tggatcacac
    63301 tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac acttctgttt
    63361 ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag atatgttcaa
    63421 atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat aaagtcacca
    63481 gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa
    63541 agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta aaacggctat
    63601 gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt
    63661 ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg atatagttat
    63721 ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa ggggttgtca
    63781 taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt gttccattgc
    63841 tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact tgcagaatac
    63901 agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag gggttggtca
    63961 agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc atttgtacca
    64021 aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccacg tttttcctga
    64081 ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg acgctgagcg
    64141 ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca ataagaatgg
    64201 gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa taggagcatt
    64261 tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa aatcaaagaa
    64321 atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct gatcactgga
    64381 gcgtaacctg catcttccag tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc
    64441 ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt
    64501 tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc gtttttttga
    64561 taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt gtataaattc
    64621 atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa aggttttctg
    64681 taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta gctatgagaa
    64741 gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc aaattgcgta
    64801 caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa gggcaaatgg
    64861 tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag acgggcgatg
    64921 agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc cgtgcaggag
    64981 aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc tatcgaacac
    65041 catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt ttgaaagacc
    65101 gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg
    65161 gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt ttgatttttt
    65221 gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga ttggaaatta
    65281 gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca cacgcgatct
    65341 tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc caaaactatc
    65401 ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact gacttgaata
    65461 ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca tggtatgcta
    65521 tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa gatactccac
    65581 gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt
    65641 acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat gatctgtttg
    65701 atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg caagaaatcg
    65761 gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa gaagatttaa
    65821 gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt tctgactctg
    65881 aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca gcctatcaga
    65941 ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa aatcccaagt
    66001 aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc atattaagtg
    66061 aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc agatcgaaaa
    66121 ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt tccgtatcac
    66181 gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata gttttcataa
    66241 agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat ggttttatcg
    66301 gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc ctgtggactt
    66361 atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg cagagctcca
    66421 tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct acttttttga
    66481 aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca cagttaaaac
    66541 gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga gagaagagtg
    66601 ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa gaacaagcaa
    66661 ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag caatcattta
    66721 taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg gctatcgtaa
    66781 tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca gaagatttac
    66841 ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat cgatctgaaa
    66901 aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat aaaacaattc
    66961 atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag
    67021 aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag aaaatcgtag
    67081 acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca gatagccatg
    67141 ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca cttccacttt
    67201 tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg cagatggccg
    67261 aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt caagaaggga
    67321 cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac aaagagcatc
    67381 aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt acaccagaat
    67441 ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat ttggatcatt
    67501 agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag taaataata
//
LOCUS       DANKAY010000004        57010 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000004 DANKAY010000000
VERSION     DANKAY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            777..1163
                     /locus_tag="PWY98_000247"
     CDS             777..1163
                     /locus_tag="PWY98_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2036035.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1257..1448
                     /locus_tag="PWY98_000248"
     CDS             1257..1448
                     /locus_tag="PWY98_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036036.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1962..2294
                     /locus_tag="PWY98_000249"
     CDS             1962..2294
                     /locus_tag="PWY98_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2036037.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2590..2745
                     /locus_tag="PWY98_000250"
     CDS             2590..2745
                     /locus_tag="PWY98_000250"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2036038.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3021..4361
                     /locus_tag="PWY98_000251"
                     /pseudo
     CDS             3021..4361
                     /locus_tag="PWY98_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4602..6218)
                     /locus_tag="PWY98_000252"
     CDS             complement(4602..6218)
                     /locus_tag="PWY98_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2036039.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6336..6554
                     /locus_tag="PWY98_000253"
     CDS             6336..6554
                     /locus_tag="PWY98_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036040.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6749..7210
                     /locus_tag="PWY98_000254"
     CDS             6749..7210
                     /locus_tag="PWY98_000254"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2036041.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7403..7642)
                     /locus_tag="PWY98_000255"
     CDS             complement(7403..7642)
                     /locus_tag="PWY98_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036042.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7666..9189)
                     /locus_tag="PWY98_000256"
     CDS             complement(7666..9189)
                     /locus_tag="PWY98_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2036043.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9207..9329)
                     /locus_tag="PWY98_000257"
     CDS             complement(9207..9329)
                     /locus_tag="PWY98_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2036044.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9359..10342)
                     /locus_tag="PWY98_000258"
     CDS             complement(9359..10342)
                     /locus_tag="PWY98_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2036045.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10366..10515)
                     /gene="rpmG"
                     /locus_tag="PWY98_000259"
     CDS             complement(10366..10515)
                     /gene="rpmG"
                     /locus_tag="PWY98_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2036046.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10536..10913)
                     /locus_tag="PWY98_000260"
     CDS             complement(10536..10913)
                     /locus_tag="PWY98_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036047.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10945..11124)
                     /gene="rpmF"
                     /locus_tag="PWY98_000261"
     CDS             complement(10945..11124)
                     /gene="rpmF"
                     /locus_tag="PWY98_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2036048.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11139..11408)
                     /gene="rpsN"
                     /locus_tag="PWY98_000262"
     CDS             complement(11139..11408)
                     /gene="rpsN"
                     /locus_tag="PWY98_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2036049.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11931..13697)
                     /locus_tag="PWY98_000263"
     CDS             complement(11931..13697)
                     /locus_tag="PWY98_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036050.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13657..15411)
                     /locus_tag="PWY98_000264"
     CDS             complement(13657..15411)
                     /locus_tag="PWY98_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036051.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15521..16090)
                     /locus_tag="PWY98_000265"
     CDS             complement(15521..16090)
                     /locus_tag="PWY98_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2036052.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16108..17523)
                     /locus_tag="PWY98_000266"
     CDS             complement(16108..17523)
                     /locus_tag="PWY98_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2036053.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17534..18202)
                     /locus_tag="PWY98_000267"
     CDS             complement(17534..18202)
                     /locus_tag="PWY98_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2036054.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18332..18916)
                     /locus_tag="PWY98_000268"
     CDS             complement(18332..18916)
                     /locus_tag="PWY98_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2036055.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19247..19480)
                     /locus_tag="PWY98_000269"
     CDS             complement(19247..19480)
                     /locus_tag="PWY98_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL2036056.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19495..20445)
                     /locus_tag="PWY98_000270"
     CDS             complement(19495..20445)
                     /locus_tag="PWY98_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2036057.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20442..21284)
                     /locus_tag="PWY98_000271"
     CDS             complement(20442..21284)
                     /locus_tag="PWY98_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2036058.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21286..22005)
                     /locus_tag="PWY98_000272"
     CDS             complement(21286..22005)
                     /locus_tag="PWY98_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2036059.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22697..22843
                     /locus_tag="PWY98_000273"
     CDS             22697..22843
                     /locus_tag="PWY98_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036060.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            22915..24141
                     /locus_tag="PWY98_000274"
     CDS             22915..24141
                     /locus_tag="PWY98_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2036061.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24229..24621)
                     /gene="rpsI"
                     /locus_tag="PWY98_000275"
     CDS             complement(24229..24621)
                     /gene="rpsI"
                     /locus_tag="PWY98_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2036062.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24637..25080)
                     /gene="rplM"
                     /locus_tag="PWY98_000276"
     CDS             complement(24637..25080)
                     /gene="rplM"
                     /locus_tag="PWY98_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2036063.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25599..26066)
                     /locus_tag="PWY98_000277"
     CDS             complement(25599..26066)
                     /locus_tag="PWY98_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2036064.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26186..27724)
                     /locus_tag="PWY98_000278"
     CDS             complement(26186..27724)
                     /locus_tag="PWY98_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2036065.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27804..28490
                     /locus_tag="PWY98_000279"
     CDS             27804..28490
                     /locus_tag="PWY98_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2036066.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28691..29218)
                     /gene="msrA"
                     /locus_tag="PWY98_000280"
     CDS             complement(28691..29218)
                     /gene="msrA"
                     /locus_tag="PWY98_000280"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2036067.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29302..29979)
                     /gene="pgmB"
                     /locus_tag="PWY98_000281"
     CDS             complement(29302..29979)
                     /gene="pgmB"
                     /locus_tag="PWY98_000281"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2036068.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(29976..31106)
                     /locus_tag="PWY98_000282"
     CDS             complement(29976..31106)
                     /locus_tag="PWY98_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2036069.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31106..33289)
                     /locus_tag="PWY98_000283"
     CDS             complement(31106..33289)
                     /locus_tag="PWY98_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2036070.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33286..34311)
                     /locus_tag="PWY98_000284"
     CDS             complement(33286..34311)
                     /locus_tag="PWY98_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2036071.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34356..35183)
                     /locus_tag="PWY98_000285"
     CDS             complement(34356..35183)
                     /locus_tag="PWY98_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2036072.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35180..36247)
                     /locus_tag="PWY98_000286"
     CDS             complement(35180..36247)
                     /locus_tag="PWY98_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2036073.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36273..37109)
                     /locus_tag="PWY98_000287"
     CDS             complement(36273..37109)
                     /locus_tag="PWY98_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2036074.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37106..37975)
                     /locus_tag="PWY98_000288"
     CDS             complement(37106..37975)
                     /locus_tag="PWY98_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2036075.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38045..39328)
                     /locus_tag="PWY98_000289"
     CDS             complement(38045..39328)
                     /locus_tag="PWY98_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2036076.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39342..41027)
                     /locus_tag="PWY98_000290"
     CDS             complement(39342..41027)
                     /locus_tag="PWY98_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2036077.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41302..42327
                     /locus_tag="PWY98_000291"
     CDS             41302..42327
                     /locus_tag="PWY98_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2036078.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42922..46596)
                     /gene="rpoC"
                     /locus_tag="PWY98_000292"
     CDS             complement(42922..46596)
                     /gene="rpoC"
                     /locus_tag="PWY98_000292"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2036079.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46654..50271)
                     /gene="rpoB"
                     /locus_tag="PWY98_000293"
     CDS             complement(46654..50271)
                     /gene="rpoB"
                     /locus_tag="PWY98_000293"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2036080.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTYKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            50999..51967
                     /locus_tag="PWY98_000294"
     CDS             50999..51967
                     /locus_tag="PWY98_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2036081.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            51985..52890
                     /locus_tag="PWY98_000295"
     CDS             51985..52890
                     /locus_tag="PWY98_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036082.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52887..53693
                     /locus_tag="PWY98_000296"
     CDS             52887..53693
                     /locus_tag="PWY98_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2036083.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53829..54818
                     /locus_tag="PWY98_000297"
     CDS             53829..54818
                     /locus_tag="PWY98_000297"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2036084.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55135..55659)
                     /locus_tag="PWY98_000298"
     CDS             complement(55135..55659)
                     /locus_tag="PWY98_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2036085.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55946..>57010
                     /gene="scm"
                     /locus_tag="PWY98_000299"
     CDS             55946..>57010
                     /gene="scm"
                     /locus_tag="PWY98_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2036086.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        17257 a        11515 c         9687 g        18551 t
ORIGIN      
        1 ctgaattcgg aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat
       61 aagacttctt tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag
      121 tattttatac gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta
      181 taaaatatcc acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg
      241 agaactcatc atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat
      301 aattattatg atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta
      361 aatattccaa tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc
      421 ttttctagtt gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa
      481 gaatattatc caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat
      541 tttcttagcc ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag
      601 tataaagata actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt
      661 cctatcaatg catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt
      721 cttttcgtgt tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt
      781 cagataacga cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga
      841 acaccgctaa tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc
      901 aactcccacc atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt
      961 taaatgtgac tctccttgtt gaagatataa aattagcaaa aatccttcct ttgttgaaag
     1021 aacctgaaca aatttttctt atcgaaaaat ttatctttga taaaactgat aaagaaattg
     1081 gaaaggttct ggggattaca agacagggtg cgacaaattt aaaacatcga ctttacaaaa
     1141 aaattcaaaa aatgctggtt taatatctgc ataataacta attttgtatg cttctttgct
     1201 cgttctcctc cttcccaaag aaatagatta taagtccatg ggaagaaggt agatttatga
     1261 attctttaaa agaagatttc attttagcaa aggctggaaa tgaagaagct gttgaggcaa
     1321 ttctcaaaag atttagctct ttgattcata agcaatcctg gagaactgga aaatatgatc
     1381 aagattgtta tcaagaatgt atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta
     1441 aagaataact caactaaggg agtctgtgac ataagtaaaa agatagtttc aaatttcgaa
     1501 caataaattt gaaactatct tctttataat tataaagtat cgattttaca gcgttctata
     1561 atgattcata gaatctaaaa cgaatgattt taattatcaa cgttttttaa ttcgttttta
     1621 gcttaaaaat aacttttgtc acagtctcac tcctaaaatt actttattta cgtatgaagt
     1681 gggcaggagg tcgaatccag aactgggcta tggtacttca acgaggactt ctcctctaga
     1741 attgaaaagc atgcgattta ccttccgtaa cgacggtaga tttattcaac tatgtgtcca
     1801 atcaaaattt gtctgatttc aaaaatagtc aagagaattt tggttgttaa aaactcttat
     1861 ctatcaactt ccacagttta gttgacaaac cctgatacta agttgggcta tgatacactt
     1921 ttcttgacta tcagttacgt taccggaagg aatgaactct attgagaaaa acatatgatc
     1981 gtaaatttaa actaaagatt tcacaggata tccttgagaa aaaagtaact accaagaaaa
     2041 ttgctgaaga atacaatatc tctcgtccaa caatttcccg atgggtttca gaatatcgtc
     2101 gatacgggaa aaatgctttt gctggacaag gaaaaagatt acctgataaa gcagatttct
     2161 acattcttga acaggaaaat aaacgactaa aagaggaaga cgagtgttct acgaacacaa
     2221 agatcgatac ggatctccag ggatcactca aaaactttac gatgaaggaa tcgaaatcaa
     2281 taaacgtgtt gtagctgttc ttatgcgtga aatgaattta tgcacaagag gatttcatcg
     2341 acgcaaatct tctcatggta gagaaaaaac aatcaaagaa atggtaaaaa aatgttgaat
     2401 cgataatttg aacagaacca aattgatgct gtttgggtga tggatattac ttacattcca
     2461 tgtaatgacg gtcgcctcta tttgtccacc tatattgact tagcaactcg tattctacgt
     2521 tactatatgg tagattcaca catgaaaaaa gagattgtcg tccgtccatt acaaatttat
     2581 taaggagaat tgcctacggt aattcattcc gatagaggta gccagcatcg gtcttttatt
     2641 tatcaagagc tgttgaacga ccaccacatc acacattcag tgagccaaac tgggactcct
     2701 gttgacaatg cagtaattga agcgtttcat cgatcaatca aatgagaatt aatcgactcc
     2761 aacaaatata aaagtaaaat agagatgaag gtgttaatcc aagattattt agaagactac
     2821 tacccaaaca aaagaatcca tacaaaattt atgatgactc cttgacaatt tgaagcgaag
     2881 aaaataaggg atctataata tctagtgttg gtgacgcctt ctgttgccaa tactttttct
     2941 tttttctaat aaacgcaaac agacacctta aaatccttta gaataaagtc gacgaaaacc
     3001 attcaaagga gtgtttcaag atgtcctatt cagagtttac caaagagtta ctagatattc
     3061 tagacctaaa tcttatcttt catgaagatg cctttcgcaa agagcgaatc aatgacgaaa
     3121 catgttttgt ttttgatggc acattgacct atcaaccgga ggaatgcttt catcgccact
     3181 atcaaaatga acaaacgatc attaaatagg gctggaaaaa agtatccatc ttattgaatg
     3241 atgtcagtaa ctacaagacc attctgcgga ttaacaaaca gcgcttcaaa tgtaaacact
     3301 gtggaaagac ttttttagca gaagattcag ttagtgatcg tcgctgctcc attgctcgca
     3361 gagtcaaaca agccattctt gagctattaa gcgaacctct ttctatgtct ttgatcgcac
     3421 gtatgaagca catttctccg accactgtca ttcgaatact tcgcagcctt cggcctaaaa
     3481 ccatttcttt gagccagctg ttaccagaag tggtgtgttt cgatgaattc aaatcagtga
     3541 aaaatgtctc gggagccatg agctttgtca tgatggacgg caagacccat caactgattg
     3601 atattgtgga aaatcggcaa ctcaatcctt tacgcgacta tttcttgcgc taccctataa
     3661 aagtccgtga acaagtgcgt ctagtggttt cagatttcta tacaccctat cgcacattgg
     3721 tgaaagagtg ttttccgaat gcccgaatcg tcgccgatcg ctttcatatt agccaacaca
     3781 ttggtcgggc ttttaccaac caccgaattc aagtaatgaa gacctttaaa aaaggggatc
     3841 gtcggtcgaa gcacttaaag aaatactgga aactacttca aaagaatgcg tgggaattga
     3901 aaggtcagca tcgctactgg cgtccgtcat ttcgagatca tctgacagaa gcagagatag
     3961 tcgatcggct attatcttat gatgattcct taaagcgagg ctacgaagtc taccaggatt
     4021 ttctttccgt tatcagaaga caagatgttc ctgaattcgt cgcgctccta aaagaagact
     4081 acaaggagtt gccagagcac taccaaccag tgttcacgac cttcaagaaa taccaaacag
     4141 aaatcaaacg agcgttgcgc gttccctatt ccaacggccc tatagaatgt ttgaataacc
     4201 atatcaaagt actaaaacga attgcctatg gctatagaaa ctttcaaaac taccgagaac
     4261 gaatcttttt atatcgagga aaatatttta agaaaactaa gaatactacc caactgacta
     4321 aagccagaac aacaacacgg ctagacaaga tagctgtcta gccgtgtgag tatctcgatt
     4381 attctattga atttcttcac caacactcat tgacgaagag ccaaaataag taatctaacc
     4441 gactgttaac taaaccattg caggtcccgt taaagatact tgtgcctgtt gtcaccgctt
     4501 ctttttcttg tccacaagcg cttaacacac ttaataaagc ccctgctatt attaataata
     4561 atatcttttt cttattaaaa acctcctcat ctatttccag attagtaaat gtagtaatct
     4621 gtgctttctt ttttctcaat actaataact aacttatggg gtaaacaaat actcgtttga
     4681 ccgggttttg agatccatcc agttctaacc gcgatttgat caggactatt atcttcttta
     4741 actcgaattc ttccatcttt aatctcaaca atattatatt gtcccttcgc aggatgataa
     4801 ttcacgagtt tatgattgat cttatccaaa tcgaagcgat ccatttcctt gccatcaatt
     4861 cgaacgatgg catatttgat agcatcttca gttgtttcat tctccttttc tatagagaat
     4921 acaaataagg gaataaaaga ggtaataatc aaaatgccaa taattatgac atcgcctatc
     4981 ttgacaccag cttttcttaa aaatttcatt aacgatatct ggctaaacat attagacttt
     5041 ttccatggaa atggtgaaat ttttcggaaa ttatctgcat taagagaatc agttaaacaa
     5101 ccctcaagtt gttccttaat acattgttga tccatcttca taccaataaa aactagttta
     5161 ttaatgcgtt caccaaacaa cggatgccag gcttccttca attcgggatt tttttgtata
     5221 atcatttgac actcaatttt aggtaaacta gctacccaat aatttaatga ttcaatactt
     5281 ttatagctac ccgatgtttc aaagatcgtc gaaaaatcac tatgttgggc aaaccaaaga
     5341 aacccttttg ctcttgtaat attgccagga aaatttttat ccgtaagatg ggcaaatttt
     5401 tgtgcgtcaa ggagtaaccg atttcgatag ataaaacttg agataccata ttcttctgtt
     5461 tcaggaatat gattttgata gccaagctct aactctttga accagcccac tgaataggaa
     5521 gtctcatcta tattaaaaag atttgtattt agaattttcg ataagtctac ttcaccttta
     5581 attgtcctaa ttattttggc attggactgt aacttagcaa ttagaccgtt aattttgtaa
     5641 atcgtcttgg ttgacactaa gtcactatta tttaaaagta atatgttaca acactcgaat
     5701 tgatcaatta acaagccacg aatatcctgt tcttcttcaa ctacattacc ttcagctttt
     5761 ctttcctgaa tcttttcacc cgtttcgaaa ttatcccaaa aacgttttac atccactact
     5821 ataataatgg caccaatctc taatcgttgg gtcaaatcaa tgtctaaagg gtttttagct
     5881 aatataattg cttgaataat tggaattggt ttgctgattc ctgaagcttc aataacaatt
     5941 tgattcaagt cagaaaattg cactaattga tctagttcaa caatcaaatc ttcgcgtaat
     6001 gtacaacaaa tgcatccatt cggcatcgaa acaagttttt ctccagttct tttaaaacct
     6061 ttcttgtcga ctaatacccc atcaacattt acttccccta tgtcatttac aatgacagca
     6121 atttcctttc cgtgatcacc gtacagaata tgatttagaa gtatcgtttt tcctgaacca
     6181 agatagccag ttaagattgt aataggtatt tttttcatag ggaacctctt tctatacctt
     6241 gaatttacac tttaagtgca acacataaaa atgagtcata atcgatatcc aactagtata
     6301 agtttgtcga gaacactcct agaaggaaaa taattatgag ccagtatcgt catcttacta
     6361 tactcgaacg agaaagaatc tttctgcttc acgaagaagg attttctatt agtataattg
     6421 gcaacgacat tcagcatagc ccgtttacta ttttacatga actgagaaga aacaagaaaa
     6481 aaactattca ccttccaaag cggaaaaaaa ttatgcttat cgaagaaaaa aatgcggacg
     6541 ttatcacatt ctaaatgatc cgtgagtttg gtcaattgtt cgtcatttat ttgtcgattt
     6601 tcagtggtcg ccagaggaaa tctcgaatcg tttgaagcat gaacatatgg acttttcaat
     6661 tagttttatc acgatctaac gtgcaatttg caatgatctt ttcgatacag aacccctctc
     6721 acacggcaat aaaggcctga tccgttcatt gcaccatcga ggaaaaacaa gacatacaaa
     6781 aaatcatgtc gagtctcgtg gaaagatttg ccttactcat tcgatccatg gccgtccaaa
     6841 atccgcagat gatagaagtg attttggtca ttggaaagcc gatattgtag ctggcaaaac
     6901 tggtggtact tgtttagtga ctttgacaga cagaaaatca tgcttccttt tagcaggaaa
     6961 gaaggtcccc agaaagaaag ccgcttatgt gagcgagaaa atgattgaac ttttaagcag
     7021 tattccaaat gaggcggtca aaacgatcac tccagataga ggaaaagaat tctccaatca
     7081 tttcgcagtt acagaagcat tgaataatat gcccttttat tttccagatc ctcatgccct
     7141 tggcaacaat gaacaaatta aaatacgaat agactcattc gagaatactt gccaaaatcg
     7201 aaagagatag ctacggtaga taactcaacg ataaatcaga tgattgaaaa actgaataac
     7261 agacctagaa agtgtttaga ttggagaaca ccttatgaag tcttctttga taaagtgttg
     7321 cgcttgattt gacaattcaa gacataataa aaagcagatc aaatccaaat aagggaaaaa
     7381 tgatctactc tggtattatc aattaattgg ccaagctatt atgccgtttt gctaaacgtc
     7441 tacttcttcc agtattgacc ttgataaaac aacaatcagg atcccgtttt aatactctat
     7501 gaactgtgtc aatactaaca tgtaaagttg cagcaatatc agctaatgta ctcccattta
     7561 atctcatttg ctttgcaaat cgataacgcc gataagcatc aatattcttg gaatccatag
     7621 acaataatac ttttcttacc atgtatttcc tccaaaaatt gaattttaat gagccatcat
     7681 ttcttgagca atttcatgtt tagacaatga agctggatag aaagtaggcc aattatgcaa
     7741 ttcattatag agtttttctt gactttctcc accaaagaaa atatggaaat gctctgcctt
     7801 acttggtgca attccatgat cactaaattg aatatattta aaacgaccag cacctgcatc
     7861 atcagtttca aaattaaaac gaacaccacg gtttcctttt tcataattca aaattttgta
     7921 tccaacatat ttgtatgtgt attgatgatg ttcaccgttg actacaaaat caatggtgtt
     7981 gtctttaatg ttgatagtat caatatctgt cttatagcca gtagtataat aatctttgta
     8041 ctcggcagct gtcatgtctt tattgatttt tgctttgtaa tcgaaaactt catccaaaat
     8101 tccatctttt aacaacggat aaacagattg ccattcgcct gtataatcac tcaattcgcg
     8161 atccttcaca tcggcatctt taaagtaccc attagcaact gtcttttcat ccttcgcttc
     8221 tttttctgga actacctctt caccagcaac catagttgtt ttttctaacg cttttagatt
     8281 gtcttccatt actgaaacat aatcttcacc attatccatt tgttcttttg taagactttc
     8341 aagaggattc agtacttcca attttatccc agcttcgttg gccaaagttt ttgcaatttt
     8401 atcattagca ttcttttcaa agtaaatata attgatctta ttttttttaa catactcttt
     8461 taattcagct aatcgaccag aagatggctc ttcttcagga cttagtccag caataggaac
     8521 ttgtttcaat ccataatcca aagctaaata tccaaaggct gcgtgttgcg tcacaaaact
     8581 tttttgtttc gcttctttaa gactagtggt atagtcagca tccaaccgtt tcagtttagt
     8641 caaatacttt tcagcatttg tctcaaatac ttttgctttt ttcggataca atttaactaa
     8701 ctgatccttg atatttgaaa cttccttaat tgccattttg ggtgaaaccc acgtatgagg
     8761 atctagttca tgatggtgat cttcttctcc gtgatcatga tcttcttctt cactacctgg
     8821 caataaaatc atgtcttttg ttccttctac tacattaggc ccagctttct tccaactttc
     8881 tttagctttt ggtacccatg attccatgtt ttcgttatga tatacaaaaa catcagcatc
     8941 atgagctttt gccatatcct tagctgaggg ctcatagtca tgaggttctg atcccgctgg
     9001 aaccattaaa tccacgttcg cttcatcgcc gacgatgttt ttggtgaaat catacattgg
     9061 atagaatgtt gttacaattt ctaattttcc atctttatca ttcgatactt ttgcatcatt
     9121 agtgctggag caagctccca ataatcctag agctgctatc ccaataagtc ctacactgat
     9181 tttcttcatt ctttttcctt ccttaattat gctaatttct tcattttatt tcgtttatgt
     9241 tctttgataa tttttaatgc cctttttcta gttttaatat tcgggctttt aagtcgtcga
     9301 tatgcactag atatatccgt tttctccatt agaaattacc cccaatcact acttatcatt
     9361 atttgctttc cgccagtaat ttatcaaaac tctgtttgat cctttcttca tccaacattt
     9421 ttccgattag aataatagtg gtttggtctt tttgtcgagt ggtgtattga aaattaacct
     9481 gttcgtttac ctcttgcatg gcaactataa aatcctgctc ttgtaaagca atcaatcctt
     9541 taaaacgata caggctccct tggttggtta aaatcagcca gtcaatccat tggagtaaaa
     9601 aatctttttt tatgtcttga tctgaagtta agagaattga tttaaactca tgtacttgat
     9661 ggtgttgatg atagtatttg ccattatagt gattttctga tgaatcactt tcaaaaacta
     9721 agggatgatt aaattttttc aaattataaa aatccttttt ttgaatcgtt tcactatgtg
     9781 tgaacattcg aatatcggat aaagggttaa tacttttgat ttctctctgt aattttttta
     9841 gtgcagataa attcaagttt tgttttctag acacaaaaat gcgatctgcc atgacaagtt
     9901 gcttaagggc ttgtggttct ctttcaataa gttcaaaact gtcactatca ataacagtca
     9961 ataaactatc gatatatact cttcctctta gttgaggcgc tgttaaaagt gtttgaatga
    10021 ttggttgggg atctgctata cctgtagttt caatgatgat ttgtgaaatt ggttgttgat
    10081 gttcttctat tgtttccagt aaagaaatca atattgctaa taaatcactg cgaagactgc
    10141 aacacataca tccattattt agttgtaaca cccgttcctc gctatgaact aataatttgt
    10201 gatcaatccc aacttcacca aactcatttt caattataat aatttcttcc ctggggtagg
    10261 gtgagacttg caaggcttga ttgattagcg ttgtttttcc agcccctaaa aacccagaaa
    10321 cgattgtaac tggtatgccc attttgtcac ctttttacca cttagctatt tcacttcttt
    10381 aaaaattgcc cgtctgcgca attttggcga atactttttt aattctaacc gttcagggtt
    10441 attccgttta tttttgctgg tgagatacag acgttctcca gtttcgacac attccaaaat
    10501 aatattttga cgcataaatc ttcctccttc aatacttatc gatcttcaag atatgcttga
    10561 atttgttgtg catcttcaaa agttaactcg ccagattcca attgtgaaac taaattttct
    10621 ttcatatcaa caggtatctc ccaataacca tcgatacatt gaaataactc ctcgtcatac
    10681 tcttcaaaaa ttggatagat tcttaaaaaa tcatcaaatg ataagccttg gtcctcatag
    10741 taatctttga ctaatccatt ttcaattgcc tgttgggtcg gtaaacccgt ttcagtaata
    10801 attcctctct ctaccatcga acgataaaaa gtgcatttat ctagcgattg ctgggtcgtt
    10861 tcaagataat actttaatcc ttcttcaact aaaggtgggt aaataatttc cattttgatt
    10921 ccctcctaaa agttatatat taaactattt tgaagctcct ttaattactt tctttccatt
    10981 atagtaccct tttaaagata catgatggct acgtctataa tcaccattcg cttcgtcaaa
    11041 tgaaatttct ggttttacta cagtttgatg agttcttctt aaccgtttct tcgccttaga
    11101 tgtttttctt gctggtactg ccatgatatt tcctcctttt accaacttgc tttttttact
    11161 ccaggaatca acccttgatg ggcaagttct ctaaacttaa tacgggacat accgaacttt
    11221 cgcatatacc ctcttggacg accatccgtc tgatcacgaa gcctcagacg attcggattt
    11281 gaatctcttg gcaatttagc gagagcggtg cgatctcctg cggcttttaa ttcctgacgt
    11341 ttatctgcat acttttcaat catcgccatt tgtttctttg ctttagcaat tttcgacttt
    11401 tttgccatat acctttatac tccttttatt ttgatttaga cacctcaaat cgtaatcgtt
    11461 acgttttgac actgatactt taccaacaag agttgctctt gtcaacaata aataacatga
    11521 aataaaactc atatgaatag ataactattt gaaaacgaat ttatcggtac atagagataa
    11581 tcaaatcatt taaaagaata tgcagtttag gctctgaaaa ataagaattc acttttatta
    11641 gtccaatcaa acacaaatac cgttttaagt agcttttcct ctacttaaaa cggcaatatg
    11701 aaagaaacta taacttttta ttcaatcttt tatgtttaac tggtttatcg ccaatttttt
    11761 aaaactttga ttattcctta tattataaga atagacatta agtgaactaa ttcaatcata
    11821 ctgattcttt cgtgtattca aaattagatg ctgtttaata aaattattct ttttgaaaaa
    11881 gaattataac tatatcccaa aaattgtagg ttaatttaaa gcctatttca tcatcgtgat
    11941 tgagcttctt tttcatagac gaatttctca gatagtttag ttttctttat gagatttttg
    12001 tatatttctg accttcgata caactcatta tgagtacctt gatcaactag ttttccttga
    12061 tccatcacta caattttatt taccttttca atagacttca agcgatgcga tataattagt
    12121 actgtcttat cttgtattaa acgatttaaa ctttcctgaa tacgtttttc attttccgca
    12181 tctaaactgg cagtaatttc atctaaaatt aaaattggcg catttttcaa aaaagcccgt
    12241 gctatggata ggcgttgtcg ctctcctcca gaaagtgaac ttccgttttc tccgaccaat
    12301 gtatgataac cgtcaggtag tttttcaata aagtcttcgc agttagctaa acgagctgct
    12361 tttttcactt cctcatctga cgcttgacta tttccaatac gtatattttc caaaatactt
    12421 gtattaaaca aagttacctc ttgaaaaaca atagcgattt tgctaaacaa agaagctgga
    12481 gatacacgtt taatatcata cccatcaatt aaaatacagc cttcatcata atcatacagt
    12541 ctagacacta atttcaagat actagttttc ccggaaccac tagcacctac taacgcagtc
    12601 acttctcctt gtttagccgt aaaagagata tgatctaaaa ttggggtatt gttatcatat
    12661 gagaaagaga catctcgcaa ctcaatgtca aaagatttca gaggactatc tgatccctct
    12721 tggatactgg tttccttcat agctctaatt cgctgtattt taggagctaa ataaaatatt
    12781 tcaagcactg cttctttcat agaatcgaag gaatccttta tttttattgc ggctagtaag
    12841 taaccaacga cgtagagtat agtcacttca cccgttaaca gtaagtgaac acctactaga
    12901 atgactactg ctaaactaac atatgagaaa atagaggata aggccatgac tgaaaaagta
    12961 cttaattcta cctttaaatg aatcctttca ctctcttcca tttttttgaa aagtctgtct
    13021 tgaacttttt tagatagatt aaagctatta atctcttgct gcaattctat agtttcttga
    13081 aattcttccg agttttctct caaagtatcg taatatttag tattggcttt cgtttgggat
    13141 ttctttgata acaagattaa gacaaaacta aataacgttg gcaaaataac agctaatccc
    13201 attttgacat taccaaccaa aagcatcact gaaataaaag ggaaaaacag agccataccg
    13261 ccggctttag gtattgcatg actcatcgca tgctcaatac cttcaacgtc agacatgata
    13321 gtttgagata aatctgataa attatgtttt gaaaaatagg ataatggtaa atttgacaaa
    13381 tcgtctgcaa tttgcactct taaatgcgca ctttctttat aagtactatt atataagttt
    13441 tcatactccc tatctaacaa ccaatacatg aaaagcaaag taacaaacga tacggcaaaa
    13501 taaagccaac ggggctttgt attttctaaa actaattcgt ctatcagcat cattataatg
    13561 aatgctggta ccatattaat gcagtataca aaaaaactag aaatagaagc tttagttaaa
    13621 gcttctgcgc cctgatcact taaagcaaat ttgtttttat aaaaactttt cattgtcaag
    13681 cctccaatca tttgcattct cataattagt gaccaattgt ttatacactt gatgtttagc
    13741 cattaattca ttatgatttc cacgttctac tattttccct tctgacaaga caagaatttc
    13801 atctacagat ttaatgctgg ataatcgatg agctatcata ataactgttt tttctttcat
    13861 cagatgttga aaagcctttt gtagttcata ctcattatct ggatcaatag atgcactggc
    13921 ttcatccata ataacaatct ttgcatcttt taaaattgct ctggcaatag ctatacgttg
    13981 tttttctcct cctgacagat aaactccttt actaccaatt aaggtatgct ctctttcggg
    14041 aaatttttct aaaatcgatt cgcaaccggc taatcttagc gcgtcaaata ctttacttgc
    14101 tggcgcttga ggatttgcta tagaaacgtt atcgtaaata ctctgattga atagtttcgt
    14161 atcttgaaag acaaaagcaa ttgtgttaat aattgtgtct tttgaatagt gatttaaagg
    14221 cttaccacca attttaatct gtcccttatc cacattgtag aaacctgaaa ttagtttagc
    14281 tatcgtactc tttccgcttc ctgattttcc tactaatgca taaattcttt tttgttctaa
    14341 aactaagttg aaattttcta aaacacactc ttctttgtat gaaaagctga cattttcaaa
    14401 tgcaatgtca caatttttaa tctgtgtctc gttcccatat tttagttggt cttgcttcat
    14461 ttctaaataa agggagtcta gtgtttcgac tgcatatctt gcttcaaaca agtgcatact
    14521 tacatacatt attctcatga aagaaacaaa aataattcca cttaaaaaaa acaacatcaa
    14581 caagtcaaca gctaatatct tgggagtggt aagagaagtt ataaaaaagg ttataggaac
    14641 tatcaaaatg caaactatgc caaaaaatag ccattgaaaa agaacaaaag gtgttttccc
    14701 actcaaagaa tacttatatg caaacttagc ataatcgtta attgctttat tcaaggcttt
    14761 aaatgatgtt actttagcac caaatatctt tataacttga attccgcgga tatattctac
    14821 cgtttcagag cttaacacat ctaacgattc ttgatatttt tgaataaaat ttgtattctc
    14881 tgtcattttc tttaacaaga atacaccaat tactaacatg gcgattagaa ccaatccaat
    14941 tttaaagtta atgaaaaagc ctaaggcaac tgataaaaca ggtgttacta tggcttgagc
    15001 actatctgga atcaagtgag caattgccat atgtgtttta gccgcgttgt catcaattgt
    15061 ctttcgaata tatcccgaag aatttaaatc aaaaaaacga aaacttgctt ctgttaaacc
    15121 gctaattccc ttttttctca aattagtctc taagcgaaac ccaaggatat gagaaaataa
    15181 gccagaaaaa atataaaata aagctcctaa agttaaccaa gcaacagttt gaaccgcata
    15241 actcttagct aaatattcat ctcctacaat aattagagac tgaagaaatt tgtaaatact
    15301 gtaatatcca tagactgtta atacgacaga aaaatcagaa aataaagtgg ctaaccatcc
    15361 gtagtatttc attttaggga cataactaaa taattttttg tatactttca ttttttcctc
    15421 ccccgcattc aatttcttct cttattcgtt tacaactgtt aatgctttcc gtaatagcgt
    15481 aaattagaga tgtacaaaca gataaaaggt aaactacgtt ctattttttg tattttttta
    15541 agacttttct tccaatcaat acaccaatca tagataaact tgctacaact aacactgaaa
    15601 tgattcccca tatacctacc gcatagtgga tcattgcatc cattgttgca gcagattgtc
    15661 ctttatctat ccaatcttga cgaaaacgat ctgtaaagaa ccatatagga atccactgtc
    15721 cggcagcact gcctaattgc attagagccc aagacgcaac ttgaggaatt gctcgatcat
    15781 atccaaatac agatagaata atatcagcta agatcgccat actaatcgaa ctgataaacc
    15841 aaggtaagta cgccccgccc attaacgtaa aaaataacaa taaaacaatt gtgtaaatag
    15901 taaaaattcc ttttctaggg tagttatagc taatataaac aaatatgatt ccagataata
    15961 atccaaaaat cccgggggat atgctgtgac caaacgcccc accaatacta ctaaccatta
    16021 ttgaaaaaaa gtaaattact agggaaataa ctgtagctaa accaataaaa atataatctt
    16081 ttgttttcat atatattctc ctcaattcta tttcgttatg tcattaaaaa tctgttctat
    16141 caaagttgaa tcttctactc taagttttat atcttttttt attccaccat tttttaaata
    16201 aataattcta ttgcatgttt tataaagaaa ttccggatca tgagtagcta taactactgc
    16261 acccttttct tttaagtctt taataaggtc agcaatcctt tccatattaa tataatccaa
    16321 cccacttgta ggttcatcaa atagaaataa attagttttt gacaatgaag ctatagaaac
    16381 caataatctt tgtttttctc caccggataa atcaaacggt aattgatctt tcattttata
    16441 aagttccatt ttttttaaaa ttttttcttg ctgatcttta ctaactattt cattccccag
    16501 ggctaactcg ctcctcactg attctgtgaa aaattggtaa tccatttctt gcattactaa
    16561 aaacggcgat gacaaatttt ttttattttc gattatcttt cctttggtag ggcgaataat
    16621 cttcatgagt attcttagta aggttgtttt ccccacaccg tttttcccta ccaaaccaat
    16681 aacatctcca actttgacat caaaatttat gtcttttaga atattttcat aagtaatttc
    16741 ttcaactttt aatacatctg cacttacctt ttcgatatag ggaacttcaa tcgaactatt
    16801 aaaaatgtca aatttcctta atgcgttatt gttttcagtt tttaacaact gaatagttgt
    16861 agtttttaca gttctatttt taacaaatag aacttgatca attaattctt tcaaatagta
    16921 aaaacgatgt tcagctataa caatagtaaa tcctttattt tttaaatcac aaagtaagtc
    16981 agcaagtttc ttagtcgctt tgaagtccaa atttgaagac ggttcatcca atataattaa
    17041 tttagagttt agcgataaac tacatccaat agaaattttc tgccgttcac cacttgatag
    17101 cgaaaaaatg tttttatcta atatatttga aagatctaat aattcagcta attcattgat
    17161 tcttttatcc atatgctctt ttgtaaatcc aaagttttcc atcgaaaata ctaactcact
    17221 cgttgtattt gttgtaaaga attgtcctct aggatcttga cttacgtagc caatattttt
    17281 tgatatactt tcttgtgtta aggattgtag cgagttattt tcaaataaaa tttccccaac
    17341 caattttccg tcataaagtc ttggaataag tccattcatt aactttaata gtgtggattt
    17401 cccactccca gaagaaccag taatcaatgt aagactgcct ttttttattg aaaaattaag
    17461 gtcaacaagc gcttgttcct taacatcctt atatgaaaaa gaaacattgt taaattcaaa
    17521 caattctttc acctcaaatc aaaatactta agaaaaaaat agtaacggaa aacaggatga
    17581 tatagtctaa taagctcatg tttgttttat agtaagaact tcttttcaca ggagattcta
    17641 tcccctttgt tattgcatta gcggtcattt cttcagcaat catagttgca cgtataagaa
    17701 cgggaactaa cagaaattcc aaatattgca gaggctgaaa aaaagacaag cgaatgtttc
    17761 tcatttttaa agattcttta atcattctta tttcagaacg ataagtcgga aagaatctga
    17821 tcactactgt aatacttaac attatttttt tcggtaaatg tattttttct aaagaggata
    17881 ataattcatt gctagtatag acactaacca acaaccatga gatcattacg accggcaaca
    17941 ttcgaaatac aatatataag gatgtcgcaa aaaatatagt aaaaaactct atcttgatag
    18001 catcaataat tttcattact aaaaacagaa ctgaataaat caagatgaaa taaatacatg
    18061 tctttatttt acgaaacatc aatactaaaa ctgtagcaat aatgaaacac cccacttgaa
    18121 atatatttcc ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg
    18181 ttaacggatt gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt
    18241 aagctgataa aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact
    18301 gagaacaatt ctcaatgcaa gcttttttta attaactcag taattcaatc cacccaggaa
    18361 cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct
    18421 tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat
    18481 taaagactaa attttgattg atcattccga tagtctttaa cttatctaga caacagactg
    18541 tctttttcgt cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg
    18601 attggaataa aatcaaatcg aatatacgtt tattctcaaa aatataatta ataaatggat
    18661 ctaattcatt gaagttctct ctccaacttt tctctatatc tatattttct aagtttaaga
    18721 ggtatttggt aaactcttca ttatacatat taataagcgc attaatatgc ggctgaataa
    18781 tttcttcaaa taacgcttcc ttagaagtaa aatgtttata aaaagcaccc gtagttacgc
    18841 ctgcggtcag gcacagttgc cttatcttag tttgctgaaa tccttctttt tcaaaatggc
    18901 ttagtccact ttctattaac ttttggtgag ttataatata atctctggtt tctatgggta
    18961 cttcctccta ataagatgaa ttaactttat tatcttacac aggacttgaa gtttcaatcg
    19021 ttaacgttta ttactgaact cattccttag agctaaacta atgagaaaga gcagaataac
    19081 tgatacaaaa agcaatccta caagaattaa tagactcatc taaatcattc accttttcta
    19141 aaataatccg agaaagattt aaaaaaatta tatccagcta ttaggataag tcctatcata
    19201 atcactccaa tagccatttt cttatcctcc ctaaaaataa agtcttttac ttaaaacctt
    19261 ttttgtaatc gtcaataaat tttttccctt tatcagttaa ttgaaatcct atatctaaat
    19321 agtcctcata tctaagttta gccagcatct cacttacaga tgctggacta acacttagaa
    19381 cttttgctaa atcattattt tttaatttct tttttctttt tgataattcc tgaattgcta
    19441 gtaaataaat tagtatagta gtcgttttaa tttgtaccat acctaccctc caaattattt
    19501 cgaaaggcct tcagcaattt tatccaggtt ccatttcatc atcgcatagt aactatcacc
    19561 atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag gaatattggt
    19621 atcttttgat actgttttca ttggtctatc atccacactg ctttctacga ataaggaggg
    19681 aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg ttccttcttc
    19741 ttcagtgttg atttcccaaa tgtaagcaga aggcacatta tacgctttcg agaaatattt
    19801 aaagcatcct tcacttgtaa caatcatttt tttatcattc ggaattgaag caaatttaga
    19861 tttagcttct ttgtctagag aatccaattt ttcaatatac ttatctagat tttctttata
    19921 gaatttttta ttatcaggat ctttttccgc taactgtttt tcaatatttt tagcgtaaat
    19981 aataccgttt tctaaattta accaagcatg aggatcttcc ttccctttct cactctgacc
    20041 ctctaagtaa ataacatcta tgccatcact tgctgcaaaa tagtctttgt tttcctcttt
    20101 gttcgcattt ttaactaatt ttgtaaacca agcatttcct ccagtttcca agttaacacc
    20161 gttataaaaa atcaaatctg cttttgaagt tttttgaaca tcttcaggca aaggttcata
    20221 ttcgtgggga tctttcccaa taggtacgat actgtgtaaa tcaattttat cttttgctat
    20281 attttcggta atatctgcta aaatcgaatt agtaactact acttttaatt tttcgttcga
    20341 agccacttta tctttgtctt tactatttat tgaacatgcc ccgaatataa aaagagcaat
    20401 caaaatcccc agacttccca gtactgcttt ccattttctc atcataacga tctctccttc
    20461 tttcttaaaa atttttgttt tggtgagatt agaaaactaa ttacaaataa gaatgccgct
    20521 gttaaaacaa tacttgatcc tgctgctatg ttaaaactat aaccaataaa caatccaagc
    20581 aatgatgaac ttgctccgac tgttgcagaa ataaatatca tagttttcaa gctatttgca
    20641 tataaatatg ctgtagctgc tggtgtaatt aacattgcaa caattaatat agttcccaca
    20701 ctttgcatag ctgttactgc tactaaagtt aatagaacca tcaatagata atgataaaaa
    20761 ctgacattca tccctattgc ttttgccatt aatggatcaa aagaagtgat taataattct
    20821 ttaaaaaata gaactactat aaccaacact aaaaccgaaa cgccaattgt aatccactta
    20881 tcaatatctt gtacagctaa aatatttcca aataaaatat gaaataaatc cgttgagcta
    20941 tttgcaactc ctataaggat aactcctagt gctaaaaaag aactgaaagt aataccaatt
    21001 gctgtatctc cttttattac actattgctt tttataaaag taataatgat agaagctaat
    21061 agaccaaata caatcgcacc aataaaaaaa ttaataccta aaatataaga aattgctact
    21121 cctggcaaaa cagcatgaga aatagcatca cccataagag acattcctct taaaataata
    21181 aaacaaccta ctgttccaga aacaatccct ataactacag atgtaattag agcattctgt
    21241 aaaaagtgaa agtcatacag accattaata aaattttgaa gcatattatt ccccctctcc
    21301 aataaatata gtatctccat aggctttttt taaattttct tcattaaata cagattctac
    21361 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
    21421 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca atttttttaa
    21481 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
    21541 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
    21601 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
    21661 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
    21721 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
    21781 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
    21841 tccctgatga gggataatat ttaacattgc tttaagcaat gtggattttc cagcaccatt
    21901 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
    21961 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
    22021 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
    22081 ttttatagtt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
    22141 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
    22201 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttgaagtgga tagcgggttg
    22261 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
    22321 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
    22381 tgtttatcgt tattaatagt gaataggaaa aaaactattt ttgatcattg acaattaaat
    22441 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
    22501 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
    22561 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
    22621 atgtgcttac ccaaagcaat tcaaaaattt cataactact attttaggag atgattttat
    22681 agaatccaga gtctacctga ataaaaaaca aacctgtgca tttctaggaa tcgataataa
    22741 tactctagat aagtggattc gtatatataa tttcccagta ataagagttg gtggtgtagt
    22801 tcgctttgac gtagaagata ttaaagactt tatgaatact tgaaattttc ttctcgccgc
    22861 atatactaaa attagtgaaa tatactgcct tacttttttt aaggagtgag gattttgcct
    22921 acaaagcttt caaatgggaa atataaaact aatctgagat atccaaaaag gtttaaagaa
    22981 ataacaggaa ttgcatcaga aaagtatcaa aaaacatttc caaataggca attggcgatt
    23041 aaagccgaaa atgatatgaa gaagaaaata gaaaaagttt tgagagagga gaatgcaaac
    23101 tcattagaat taaaaggaaa aataacgttt aaaaaatttt acgaatctaa atggcttcct
    23161 agatatgaat taggtcaaac aattagatca aacaggcctc ctagtgatat aaccattagt
    23221 aatacaaaag atatatttcg gttgcatatt ttacctatgt ttggtgaata cgcaatgaat
    23281 tatctaaata tcaatactga gataatatca gatgaattaa ccaaaaaatc gaaagaatat
    23341 gcaaatataa aaatcataaa aggatatgtt cgttccatgt tcgatattgc agaaatacta
    23401 aattatatcg aatttaatcg aactactaaa attattcaaa gtatcactgc acctaaaaaa
    23461 aatgctttag aagaaaaaag aatacaagaa ggtaagcaag cactatcatc aaaagaactt
    23521 actaattgga tagaagcagt aaatgatgat tttaataatc atttacttac ttttcatgat
    23581 tacacacttt ttatgattac cctgtatctt ggtgatagaa aaagtgagac ttacgctttg
    23641 caatggaaat atattgattt tgaaaaacaa acggtccgtc ttaaacatac tttagataaa
    23701 tatcaaagaa aaaagtttac aaaaggtcga aaagataccg tcattcaagt accagaagtt
    23761 gtaatgacat tgctaagtga atggaaatcg gtacaagctg atcaattgtt gaaattaaaa
    23821 attaagcaaa cgttagatca gtatctcttt acttatacta aaccatctgg agaagtaaat
    23881 tgtcctgttc atgccgatta cctaaactat cgaatcaatt caattaaaag aaggcatcct
    23941 gacttagttc atttatctcc acacaaactc cgtcatactt atgcaactat agctagacaa
    24001 ggtggagcag atatgaatca aatttcaaat gctttaacac actcagatat ttctacaaca
    24061 aagatatatg tgaacacacc agatatagtt gataaggctg tttttgaagc ttttcagaga
    24121 ggattgaata agtgtgattg atatgtgttc ttaaatgtgt tcttaaaaac aaaagaagca
    24181 ctttccaaat aattgaaaag tgccagaaac gttgatatat aaaggatatt aacgttttga
    24241 gaattgtgaa gctttacgtg ctttcttaag acctggtttt ttacgttcga ccatacgtgc
    24301 gtcacgagta agtaagccag cacgtttaag agctaaacgg aagtcaggat caacttctag
    24361 caatgcacga gcgataccat gacggatagc tcctgattgt ccagcgtatc ctccgccgtt
    24421 tacgttaaca aatacgtcat atgcgccttt tgtttcagta acagcgaaag gttgattgat
    24481 tacttcacgc aagtcagcat gtggaatgta ttcttcaaca tcttttttat ttacagtaat
    24541 tttgccagtt cctggtacta gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc
    24601 gatatattga acttgtgcca atgaaattcc ctcctattag attaagtttg tgatgtctaa
    24661 aacttctggt tgttgtgctg catgtggatg ttctgctcca ccgtatacgt tcaattttgt
    24721 aaattgtttg cggcctaaag tgtttttagg aagcatacct tttacagaag tttcgatcaa
    24781 acgacgagaa tttttagcac gcaattcgcc agcagtgatt gatttcaatc ctcctgggta
    24841 gttgctgtga cggtaataaa ttttgtcagt cgctttttta cctgttaatt tcactttgtc
    24901 tgcattgatg acgatgacga aatcgccagt atccaaatga ggtgtgaaag ttggtttatt
    24961 tttaccacgt agtacagatg caacaactgt tgaaagacgt cccaatggaa catcagttgc
    25021 gtctactaca taccatttac gttctacttc gcctggtttg gccatatatg ttgtacgcac
    25081 gttattttcc tccaatttaa gttctgatgt ttgacataca cttcgagttt ccggggctct
    25141 ttgtggggca aacaatacca tttaacatat tacccgctaa tctagtcaat gtcaacgttt
    25201 ttctgcttga ttgaaaaatt tatatgaaac tttacttctt ttctttcttt tatataaaag
    25261 ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc
    25321 ccttattttg aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca
    25381 taaaacataa gaagctgttt tttaaaattg tttcttgtga ctcggagctg aacggcaggc
    25441 ttacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    25501 gcaaaatatg aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat
    25561 ttcacaacct ctttttcggt caagttcaac aacagttttt acgcgtcgat ctctggcgct
    25621 ttgcccaaat gagcttggat catccagata cgtttttctg tggctgtttt gaattcgata
    25681 aagatatctt gtgttggatc gtcgccttct tctccagaaa cttcgattcc tttttggtaa
    25741 agatctgcta gataacgata gcctgcaaca agtgtttcca aacgttctgg aatagtacga
    25801 tcccagcggc caatttcatc agggattttt gtgttatctg caaattcacg caatgtagaa
    25861 taaggtgaac cgtctaatgt gatcaagcgt tcggatactt catctaactg agcgttcaaa
    25921 tcatccatga actcatccat cattgggtgc aatgtaagga aacctggtcc tctcatgtac
    25981 caatgtgttt ggtgaacaac aacagacatt tggcttagat ctgcaactaa ttgatttaat
    26041 acttctttgg tttgttggaa tttcatcttt cttgcctcct aacattttct tacacttaaa
    26101 ataatagtag actttcaatg aaaagtacaa caaaatcatt tacctgagaa cgattttcag
    26161 ttcgcttttt tatccgtaaa gccaatcatt tttcagaatc agctaattct tctcgcagtt
    26221 gagcaaattg ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat aatggataga
    26281 ttttctgata ttttttatga tcagtttctt gtactccaac ttgctctttc aattctatca
    26341 attcctgtgc ttcactgata ctttggatct ttccgatgct tctccagcct aaaatcacag
    26401 ctcctaatgc ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc agtacatcag
    26461 ccatcatctg acgaaaaatc ggagattgag caaacccgcc tgtagcacgg atctctgtga
    26521 gctgattttc ctggcaaact ccttttaaaa tccgataaag gttgagacag atcccttcta
    26581 gaatcgcctt catcatttct ttttctgtat gttgccgctg taaaccgata aaactgccaa
    26641 acgcatctgc gtcccaaagc ggtgctcgct caccaagtaa atagggatga aaaatcagac
    26701 cattggcacc cgctggcaca aaagcgatag ccgaaagcag tgcatcatat ggattttctc
    26761 ctttttcttt agcagattga acgacttcct gtaaaaatgt atgccctgcc caatcaaaga
    26821 tgccagcacc attgctagtc gctcctccca ctacccaatg tttctgatcc agaacataac
    26881 aaaacgtctc catttctgga tggaactgag gctgagtagt aataaaacga agtgctccgc
    26941 tcgttcctac ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct agattagaaa
    27001 gcggcccgtc tgcccctccc aaaacaaatg atatatcatt tggcaaacct agttctgctg
    27061 ctgtttttga tggtagacct gttacttgat aggtggaagg aaagacttgc ggcaattgct
    27121 ctattcgaat gttcaagtag tcaagcactt cttctgccca ttggaattca tggatatcaa
    27181 aatatccagt tcctgaagct gttccgtagt cgctgatcag ttcgccaaac aatctataaa
    27241 agatatagtc tttgataccg acaaaataag cagttttttt gaataattca ggttggctgt
    27301 tttgcagcca cttgattttg ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc
    27361 gataaaattc tttgccaagc acggtatatt tcagtttttc tgcttcgtcc gctgctcggt
    27421 tatctgccca agtgatcatt cgagataacg gtgttttctc ttcatccaac aatatcaggc
    27481 catgcatcgc agaggagaag gagacaaatt gaatgattgt gtctgaaggc aactgtttaa
    27541 ttacttcttt tataacggtg cagacacctt gaaagatcaa ttcctgatct tgttctgcca
    27601 tcccttgtgt ttcttggatc aaagggtagc gaaaataagc ggatgctgct tcttgggctt
    27661 catctgtgaa agcaactgct ttggtttgtg tggttccgat atctaatccg attgaaacgt
    27721 tcattttttc acctcaaatt ttatgtatgt acaaaaattt agtttaagct tgcgtatctt
    27781 ggttttgaaa agggggagat ggcgtgttac tttattttat cattggttgc tgtatcggat
    27841 catttttatg tttaaccgcc cagcggattc cgttaggtca ctctattatt tatcctagat
    27901 ctcattgtgt caaatgctgt cgttcgcttt cttggtatga attgattccg attctctcga
    27961 tcatcgtcca acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc tactatttgt
    28021 tggcagaagc cttatgtggt ggtctttttg cctggttctt tactttttct tccgcaagaa
    28081 atttttcgac gttcatatgg atgctttcgg ctttgctatt ttcgttgatg gatttatttt
    28141 acttcatggt ccattctcac acgctattct gttcttgggc aattttatgg attttttggt
    28201 tgcaggccgg cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt
    28261 gccttcgcta tggtcaatcc tatctcggag ctggcgatac tctgttgatg ctttcttgga
    28321 gtggtggctt gtctctagaa gagttgcttc agatcttgtt tcttgctagt ctgtttggaa
    28381 tatccgtttt ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca
    28441 ttcctttttt gagcagtgcg ttgctgattg tccttcatct taggtattga aaaaaggttg
    28501 tgacaaacga cttgtcacaa cctctgatat atagtgttct cttaccaaat ccttgtgact
    28561 tggagctagg cgacaggttc acaacctctc cactaaatgg tgttcaatca gctgaccctc
    28621 agtattagct caaaatgctg gttttacaac ctctgatata cggtgttcca taagtagctt
    28681 tttcggcttc ttatttcttg acgccccaat gtcgacgaag gaaacctgca cgtccagaac
    28741 cttctcgata aagattgtaa tggagcttgt ttttcttgta ataatcctga tggtaatctt
    28801 ctgcaggata aaattcgctt cttggttcga tttttgtcac gattggctta tcgaatcgcc
    28861 cgctgtcttg caatgcttgt tttgaacgct ctgcaatatt tcgttcttct tcatcattat
    28921 agtagatcac tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg
    28981 gatcggtttg ctgccaatag atttccacca actgatcata agaaataact tctggatcat
    29041 attcgatctc tactgcttct gtatgccctg ttgtgcctgt tgttacttgt tcataagttg
    29101 gatttggtac atgcccgcca gtataaccgg atgtcacaga aatgatccct ggctgctctt
    29161 caaatggttt gaccatacac caaaagcatc cgcctgcgaa aactgctttt tttgtcatat
    29221 aaaatccccc ttttttctca tcataccttg atttccaaaa ggaggataac gttttgcttg
    29281 tgacagaaaa aacggaaact attatatacg tgatacggct tctgataaat gccaaaacga
    29341 acgtacttga tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag gaagatttat
    29401 gttttcacct attcccaata catgcatgtt accagccaaa gctgcttgac aaccagcttc
    29461 tgaatcttca atgaccaagc atgcatttgg cggtacattc aattgctgtg ctcctttgag
    29521 aaaaacttct ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa
    29581 ataacttgtc aaattcgttt tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa
    29641 tccgatcttg atgtttgttt ttttcaagta ctcaagaaca gctattgcac caggcaaaac
    29701 ggatgtttca tccatttttt gaatcgcttg aagatataga ttgttttttt gctcagcaaa
    29761 ggctgatttc tctttttctg agaatttctg ttccattttt ccaaatgaca gcaatcgctc
    29821 tagtgactcg gttctggata ttcctttcag ttgctcgttt tctttctcag tgaatggaat
    29881 ggacagctgg ttagctaaaa ccagccacgc ttcgtaatga taacgagctg tgtctacaag
    29941 aacgccatct aaatcgaata gtgctgcttt gattttcatc gattacctcc ttgaaaatga
    30001 acgacttgaa gtaaagatgc gatgttttta gctgtgcgat gaagattatc agtggcttgc
    30061 tgtaaagctt cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca
    30121 acatctgctg ctttttcgct tcctttacca acacttccgc aaatagctat cacaggaacg
    30181 ccctctggta ctcttctagc aatcccaact ggcgcttttc cttttagact ttgtccatct
    30241 agacgtcctt cgcctacgat tacgaggtct gcttgctgta cccgtttatc gaactccagt
    30301 aaatccaata cgtagtcgat gccagagact attttccctg aagcaaatgc taacagtcct
    30361 gcacccattc caccaccagc actactgccc gatgcctcga ttacttgagg ggcaaatctc
    30421 atataaaaat catgcatggc ttggtcagcc actgctaatt cattttcttt caagcctttt
    30481 tgtggtccga aaacaaaaga agcgccttct tttccgcata aaagattagt aacatcagta
    30541 atgacagtga ttttggcttt aatcagctca ataggcacgt tcgttttatc aactgtagca
    30601 attttaccta gattttctcc cagtgcttct actttttgat tatcttgatc gtaaaaagca
    30661 taacctagac cttctgccat gccgatgccg ccatcgttgg tactacttcc gccaacgccg
    30721 atgatgatgt gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc tactcctcgc
    30781 gtggataacc taagtggctg ttgttggttt agtgggacat ctttcaatcc gacgatttct
    30841 gccatttcaa aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt gtttttgcca
    30901 aatgcattag taacgactgc tgtttgccgc ttcaacctta aactgtctgc taatgatgaa
    30961 accgttcctt ctcctccatc gcctataggt agcagatcat attcagcctg aacaaaaact
    31021 tcttgaaaac ctgattgaat ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa
    31081 gaatctggcg caatcagaat acgcattatt ttcccctccg ttccaagatc agttcttttt
    31141 ccagctggta ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta
    31201 ttactatttg tttttttgtc aaaacaaata gcaacctgtg atttttccaa ataaaaggaa
    31261 aagaaatttt ttcccatgct gaaggaagtc ttggagaaat agaaagagtt ccattcgtta
    31321 ctgtaatacc tgaaaaacca aatataactg ctagccatag tgctcctaat gaagcagcat
    31381 gaattccgtc atctgatgaa tgcggatctg gtcctaaatc aatcaggcac gcttcttgga
    31441 ataaatgata cgcccattcc atttcttgga tacggcaagc ctctatggcg tgaatcgctt
    31501 tactcaacga tgagtcgtgg attgttttgt tttcatagta atgcaaattc ttagaaacga
    31561 ttggttgaga aaaatactca ggtaataagt ataacagcat gacaagatct gcttgcttgc
    31621 tgacttggag ttcatttact tcgctacggg cataatcaag taaaatagac tggctgccag
    31681 gacgttcttt gtaagtggtc aaatcaataa ctggcttact gagatacgtg tcatcttgcg
    31741 gaatcagctg ttctttgtta ggcactggca aatataactt atctaaaaaa ttttgacatt
    31801 ctttcaaaaa aagcggatcg ccagcttgat atttaacagc taaggccaca ttataatgcg
    31861 ccagatagtt cgtatatgca ttattgtcga tatattctgt gtattcatct ggaccaatga
    31921 ctctgtgtat ctctaaatga tcatcgactt ttacagctct gctcagccaa aaagctgctg
    31981 tttcatgaat gagcgttttt ccctttgttt ctaaaaacga ttgatcctct gttgcttgga
    32041 catactcttg aacggcataa gcaaggtctg cactgatatg atgttctgcg gcagctgaag
    32101 caacttcttg tcttgttccg gttttgatat tcaatgcagc aaatttgggt gtctcttcct
    32161 gaccagtgtg tgcgctttcc catgggaaaa gtgcaccttg atagtgataa tctcttgctt
    32221 tttgccgtgc gccttctaat cgatcataac ggtacagcag caaattcttt gctacttcag
    32281 gaaactgata taaaaagaaa ggcaggataa aaatttccgt atcccagaaa acatgccctt
    32341 tataaccttc gccagttagt cccttcgccc ctacactaac acttggatca ttgcggggag
    32401 tcataatctg taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc
    32461 cttggatcgt tatccgatgc tgatgccaaa aatctgtcca ataagacatc gactgctcta
    32521 aaatttctgt ataactaacg cttttctggg cttgggcaat acttttctta aacgggtctg
    32581 tttgctgaag agaggtagag agcacgatga tcttctccag cgtaaaggac gtattttttc
    32641 tgatcttttc tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat acacctgctt
    32701 tattgaattt agctgcaatc ccaatatcaa ttttactttc tgttgtctgg taggatgcac
    32761 tgatcaaatc gtcaccaaat actcgcagtt ctttttcaac caactgctgt gtaccaaaat
    32821 tggtttgctg tccatcgata cccgttttca catctacttg gcagtcttcg gtcaacggct
    32881 tgatgaccac tttcagggct gcctgatgga gattattttg atcagcaaca cgacaagtaa
    32941 tgacttccac tttttttcct tgagcagttt caatgagcgc atagcgttta agctctccca
    33001 tttctaaatc tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt
    33061 caccgtcgat ttttatttcc catctgatga catctgggag attcactaac tcggctgatt
    33121 ctgctcctaa aggtcggtta tatacaccag ctgcaaacat tccccttact tgattccgat
    33181 agtcttcctc ctggcttcct ctgataccaa aaaatccatt tccaatggtc atctgactcg
    33241 catacttatt gatatactct aaatcaaact gtttttcttc tactttcata aggtaataac
    33301 ttctcctgtt tctgccgaac gatataaatt ttccaccaga ttctggatga catatccttg
    33361 ccagccatct gcaatcatct gagtctggtc accgagcgcc cgattcacaa aatgagccat
    33421 acttagttca tgtgggtcat taactggcgt ggtcttttgt aaggaaacca gctttccatc
    33481 tacatcttcg aatagttcgc ctggatacag attagcacct gcctgatcgc cacaaaaagc
    33541 cacattcagt ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt ggatgatctt
    33601 gccgttttct aattccagtg tgccaaaaat tgcatcttct actgtgaatt tgcttggatc
    33661 ccatttcccg aatttccctg aatttttttt cgtaccaatt ttttggaagc tgtacgcccg
    33721 aacgcttttt actttaggaa atccagtaat atacatcgct gtatcaagca tgtgaatgcc
    33781 ataatcgatc aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc cccagcctgg
    33841 aacaccagac cgacgaagcg cattcgcttc tacgtaataa atatccccga gaacatcaag
    33901 atgattcttg atcattgttg cttcgtcaga aaaacgatgc tggaatccat aagccaaaat
    33961 ctgcccgttt ttatcggctg tctctgccat ctttcttgct tgatcagctg tcatagctgg
    34021 tggtttctcg cacatcactg aagcattagc ttcgagggca gccaatacat gttcgaagtg
    34081 aaagcgattg ggtgtacaga cactcacgat atcgggagaa atctcattca gcatttgtgt
    34141 aactgatgta tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct
    34201 gactggatca accacagcgg ccaattctaa gacatctgat cttttttgat aataagcagc
    34261 atgtactttt tctgctactt gtccagcgcc tatgattgct actttcttca tttatttctc
    34321 cctcctttgc ttatttactt attgttattc tttctctatt ctttctctaa atcatttaaa
    34381 tgacgcgctg tttccagaca gtttttcaag tattgcaatg attcctgata aattttcact
    34441 tcattttctc ctcgcaaacg acattcatat accatataac cttcatactc attttgaagt
    34501 agcgtttgaa aatggcgagt aaaatcaatt gcgccagatc ctggttggta gcgatgatta
    34561 tctgctaaat ggatatgacc cacatactct ttttgcgtag tcaatgcttt tgaaatatca
    34621 tcttcttcaa tattcatatg ataaaaatca gcgatgatct gcacatgttt caattgatta
    34681 gagtcaatgt aatgacgcgc atccgctaac aaattgatca tatggtcttg gtatcggttc
    34741 aaaggctcca aataaataac cgtacctgtt tcttcggcta ctttatctaa ataacgaagt
    34801 gattcgctga ctgctttata gtctccttct ttgcttcggg gtgaaaccat cggcggtaat
    34861 cgataagtga acattcccca agctgctggg ataacgattc ctttgcctcc aacctcgctt
    34921 agtgcacgca aaatctcagc aatttgacgt acaccattaa gacgccgttc ttcgataaag
    34981 tcaccgatcc acccatcgta gccgttacaa gctgtcacta ctgggatccc tgtttctttg
    35041 attgcctgtt tgactgtgtc gatattttct attaacaact taccgtcgat ttcgtattct
    35101 tcaaatccac agtctttgat aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct
    35161 tgatcttgcg taccgaattt cattgttctc ctcctcggac ttgaacgccc attttgatac
    35221 tttcttctgg gtgctggtct acataacgca tgtaagcttc tgcgctttct gcaaatggca
    35281 caactggatc aatgatttct tcacaatcca agtaaccgtt catcaataat tcccaacatg
    35341 tgtcctcgat tcgtttacgg ttccatcttg ggtaatctgg gtttggttca ctagatgctc
    35401 gagcaaagac gatttttgca ttattgaaat gtgcttcgcg accaagatta aacccagctg
    35461 gaaaaggttt agcaaaggca acataagaaa tgatcccgcc atagccgatc cctcttaaag
    35521 aagcttgtaa tgcggcagca ctgccactcg tttcaataac tgaatctact ccacgtttat
    35581 ctgtcaattc ctttagacga aaaccgacat ctgctttcat aggatctagc gtgtaatcgg
    35641 caccgtattt ttctgctaat tctctacgct gcgctatggg atcgatccca acgatggtag
    35701 tagcacctgc ttttttagct aattgaatcg caatttgccc gatagcgcct aaaccgaaga
    35761 ttgctacagc atcacctgct cgaacatgtg cttcccggat accacctaaa gcgaattgag
    35821 caggatcgta acatactgca tttttccatt gatccaaccg atcaagttta cgtagtttat
    35881 aattatcaac accgtttgta atcagcgttt cacgaatggg tccataagtg cagactaact
    35941 ctcctttttg atactctgtc accaaactgc ccgtttcttc gatcgtaccg acaatcatat
    36001 ttcctaattg aaattcgcca aataccacac cgcgttggct tccttcttct cttggcaaga
    36061 ataggcgcca ttcttctgaa aattcttcat cgataaatgg acttaatcct ctaaagtcga
    36121 caatttctgt tcctggtttt ggagtagcga aaattactcg gatacgtact tcgttctttt
    36181 cgatctcacg gtcttcgtaa tctttgaatc cggctattct tggttctaat gcaactaatt
    36241 ttttcatctt ttctttcctc tttcttttct ttttatcctt ttacgccgcc ttcgactaaa
    36301 tcacctttaa taaatcgttc agataacgca tacataatga tgactggtaa agcggtcacc
    36361 aatgaagcgg ccatcattcg tccccaaatg tagtctggtg tactgaataa tgtgttcaaa
    36421 ccgattggta aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca
    36481 ttccacgaaa ccataaagca ataaacgaag acagaaatga ttccagaaat agccaatggt
    36541 ataatgatcc gaataatgat ctggatccgg ctaagaccat cgatcattgc tgcttcttca
    36601 atatcagtcg gaatcgtgtc aaaataactt tttagcatga atatcgcagt aggaagtgtt
    36661 tgaacgacca tcgtgataat cagtgctgta ttggtatcat acaaacctag accggaaatg
    36721 attttaaata atggaacaac gatcaaaata ccggaaaaca tataaacggt gtaaaagcta
    36781 gcattgattt tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc cccgctgatc
    36841 ccaatcacta ctgcagttac ggaggctatc aaggagatca ctaaactatt tttgaaatac
    36901 atgatgaatg gaaaaatctc agcattgaaa atatcctgat aatgttcaaa agtccattct
    36961 ttcggaaaaa tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc
    37021 agcatgatca aaaatggaaa caaattgaca gctaatagca acaaaatcgt cgcgtaaaat
    37081 aagattttcc tgctcttttt atttttcatt tcaagtccac cttctttcgt gagagcaaga
    37141 cgaccgctgc aataatgatg aacaatagaa tcgatatagc tgctgctttt ccaagatcat
    37201 tgaacgcaaa ggctgtctga tacaaataaa cgcctaagat attgacttta ttcgtcagta
    37261 gatagacatc tgtaaacata tagaacatcc agatagatcg taaaacaatc acagttgcta
    37321 gtactggttt gattgccggc agtgtcacga tcttgaactt ctgccaagca tttgcaccgt
    37381 ccatttcagc tgcttcataa agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa
    37441 taaacgcata cggatagtat ctccagatag caaacaaaac gaccataaag aagctagcaa
    37501 ctggctggtc gaaccacagc ggtgcttcgt tgaaaagctg caacctatct actaacagat
    37561 cattgacgat tccataacta ttattgaaca tatatttcca agcaaaaatc aaagaaatag
    37621 atggcgtaac ataacttaat aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc
    37681 ggttaagcaa aatagctacg gctaatccta aagctgtact gcctgctaca actaagaatg
    37741 tgtaagcaac tgttaaacct aaagaacgat aaaattcact atcacttaaa atttcaagat
    37801 aattgcttaa tccgataaaa acggcgtctg agttaggatt cagcggcata tcgaaaaaac
    37861 tgatctgaat attggaaagc attggaatca gcactaaaac agctaataaa agcatgcttg
    37921 gtgctaaaag tcccatggct aattttaagt cagaatgttt ccttggacga ttcattgaaa
    37981 cctccccctg ataatctgcc aatagctatc tacttttttc gaacaccatt ggcagaactt
    38041 cttgttattt cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga
    38101 cagttacttg gttcactgct tttccgatca cacctgagct tgtgatatct cccattttcg
    38161 tgaaattctt ttccccgact agtccaaaga cttgtacttc atcaaaagca ctagcaatct
    38221 cagatggcag ctcgccaaaa gacttgatga cttcattttc tttgtaacca tccagttcga
    38281 ctacttgttt attcactggt tgagcaccac caggagacat caagacccac ttttccatgt
    38341 ttttcggttg agataagaaa gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta
    38401 gaccagaaga aatcgtcaat ccagatactg tgccatagac agcttgatct ttttgtgttg
    38461 ggatagcaaa accgatattt tctgagttcc ccgcttcata aacagaaggc aaaatgtaag
    38521 ttgaatagat tgccattggt gctgttccat tcatgaaagc atctttgatt tctgtcacat
    38581 cattagagcc tggcatcgtg tattgggata agttttgata aaacgacaat gcttctctca
    38641 tcttttccgt atcgatcgtt acctcgcctt catcatccaa aacatttgct ccatttgaca
    38701 aagcaaattg agagaacgct tgctcactca ttgttccttc agcagtaggc atagcaattc
    38761 catattgttt attctcttta tctgtgaaat gttgagcaat ttttaaaata tcatcccaat
    38821 tttctggttc tgaaaaacca gcttctgata atttttcttt gtcataccaa ataccttgaa
    38881 cccagccgct aattggtgca gcaatatagc tttttccatc ttctgaacga acaaggtttt
    38941 tggctccgtc ataataatta tcttctccag cttcctcgat aacgttttgg actgccgtct
    39001 gatcaatcag ttcatcttta tccatcactt tagcaaaatc ctgactgact tccattactt
    39061 ctggtaattt accggaacga gctaacgtaa cgatttttgt attgaacgct gattcttcta
    39121 ccggcacttg cttgattttg atgtctggat tttctttttc aaaatcagca accagttcat
    39181 tgatcacact taatcgttcc tgttcgacag aagagtgcat gaattgaatc gttgtctgtt
    39241 gtgattcgtt agagctttga ctttcaccac aagcagtaag aagaaatcca gtagctagca
    39301 gacaggtagt aacaataatt tttttcattt ttttccacct cttattttat tattttcagc
    39361 cacatatatt catacggctg gaatatcaac tttcgttctt gactgcaatg gataatttgt
    39421 ccagaaaaca ggtctaggta atctcctttt ggtaaaagat aacctgctga ttgattggaa
    39481 aaattatgga taactaagac tttctcttcc ttcgcttcgc gaataaaact gataaaagaa
    39541 tctgctgagt ccagtaagta aatcaaacta ttcggatgga aggctttttc tttttttcga
    39601 atagatagca tctgggtcaa ttcaccatat actgctgttc ttaatggatc tgtctgcaac
    39661 tctttctcaa tatctgctaa caaaaatttt tgccgattga tttttcgatt ctctcctgtt
    39721 ttttctacgc ctttgtaatc atttcgactt cccagcacac tttgaatata aattgccggc
    39781 acgcctggta acgttaccaa aaatgaatga gcagctaaaa agcgtttgct tctccattcc
    39841 gtcgtatctg ttggagaact taacgcatct agatacgtag cgtttatctc atatggactg
    39901 gtcgttccat ctgggttttt cttgtagttt actcgggtac cctctttctt cagctgttcc
    39961 actaattcaa ggatcgtttt ttccggcaga atacctcgta ccggattcaa tccgataccg
    40021 tcatgggagg ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt
    40081 aaccattctt tcaacttact tgtattaccc gaacgaatcg cttgaagggt caaaggtggt
    40141 agcgaaaact gatagaccat ctgtgcttca ttctcgccat tcccaaaata actgatatta
    40201 tctttgtgtg gcacatttgt ctcagtgatc aataccgttc ctggagctgc ttcatcagcg
    40261 attgcacgga ataattggat gattttatgc gtttctggca ggtgtataca agaagttcct
    40321 agttctttcc ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc tttttctaag
    40381 taaaacaaaa gaatatcgat cattttcaat aaaacttctg gatttttaaa attcaagtct
    40441 acttgatctt cgctaaaagt cgtccatacg tgtttttctt tcccgttttt gtcaatgaac
    40501 gttgtaagta acgggctggt ccttggtcta gtcacgctgc ttgtatctgt atccagtggc
    40561 aaatcaataa aaaaatcttt gtacgtatca ttcaagttta aatattcctg aaaccaagta
    40621 ctttcggcag acatatgatt gcagacaaag tcgaacatta accgtgtttc gcttctcatt
    40681 tcttctatat cttcccatgt gcccaaacgg tcgttgattg cctgataatt tacaacggag
    40741 aaaccatcat cagatgtata tgggaaaaat ggcaagaaat gtacaactct aaatgtatct
    40801 gccaaatact tttgataaaa tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa
    40861 aattgatccc cataagtaat caagaaaata tcttcttcgt cccaatgcag tttgcctgcc
    40921 tggcctttct tctgctgata aacactgact ttttgttcaa tcatagaggc ggttttctca
    40981 gacaaatctg agtcgcgata aatcgcttgt aaaagttttg tatacatcgg attccccttt
    41041 cttttttggg agcgttccca acgatttgat catattatgg aaaacgctta ctgtcaatag
    41101 cattttttta aataattttg atatacacaa aaatacaaat aagaaagctt atgtatcaat
    41161 aattaatagc ttcttaaaaa aatatggtct ctcatgatca tgcaagctat tcctaaattt
    41221 tcaaaatcat agacaaaaaa gaatgtctat tgtactatgg aattattcta aaacgctccc
    41281 aaaaaggagg tgcatataaa attgaaacct actatttatg acattgcgaa aaaagctaat
    41341 gtctcaaaat caactgtctc cagagtatta aataaccagc caaatatctc tgataaagca
    41401 aaagcaagtg tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga
    41461 ggactgtcag gaaatggttt tgacgcgatt ctagcattat ctcatcgtac ttctcatacg
    41521 acctatggca atcctttttt ttcagatgtt attcaatcca tttctgccgt ttctgaacaa
    41581 aataacttcg acttgattct acaaacggca aaaacaccag aagaagagct agaaaaatgc
    41641 gcgaaaaaga tccaagaaaa aatgatccga ggaattatta ttttaagctc tacgatggat
    41701 gaaacacttc tttttgaatt ggatcaatac aatattccta ttgtggtaat cggaaaagtc
    41761 ggaaacgact acacgaacat ctactcagtg gacacggata atttccagga tagttacgat
    41821 ttggtggaaa cactgatcaa gaatggacac cgtcatatcg gctgtctcca tgctccgaca
    41881 gaagtttatg tatccaatga tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac
    41941 ctagatatcc gtaatgaatg gattatagac ggcggctttc gcttagaaga tagcatggca
    42001 gcaatgaatg atttaatgaa gctacgagaa agaccaactg cgattttttc taccgatgca
    42061 ttaaaatcat tgagtattta caaagtggca gaacaatctg gaaagcagat acctgatgat
    42121 ctttcgatcg caggattcag tgatccctta ttgtcctccc tattttcgcc acagttgact
    42181 cggatagaca ttccgacaaa tcagttagga atcaaagcca cagaactttt attccaacta
    42241 atccaccacc aataccctga agaaaaaaga atcattcttt caaccgagat tactctcaca
    42301 gattcgatcc aacaacaaga aatgtagaac agaattataa aagaacagaa acactgagga
    42361 taagtcagtt actctgttct tttttcaatc actcatccct cgttaccccc tcccggtatt
    42421 ttagcgaatt tttcttattt tccatctgtt ttagatagct gtggataact ataatgtttg
    42481 tgttaaacta ttttttccac agggaagaaa aaactatttt taaccacgtt gtgtataagt
    42541 ttttatagtt cgttttttta aacacattta cccttatttc aatccatgtt atttggggat
    42601 acttatctct aaaacataaa tagaataaca gacatgtcta ctggttttgt tttattcgag
    42661 gaattattca ataatagcta gcaaaatatt aaaaataacc gctctatctt cccatcaatt
    42721 tattttgcat tcaaaaaatg atctatcttt acttttgttg attgacttct cttggaagat
    42781 gatcgttccg ataccttaat tggatacgaa ccgatgttgt taagatcctt tcccactttt
    42841 cttctagtta gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc
    42901 acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat
    42961 ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg
    43021 ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac ctataataac
    43081 attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac
    43141 acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt
    43201 tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt
    43261 gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc
    43321 acctggatcc attacacgaa ctttacgcag catttgacga accattactt cgatatgctt
    43381 gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt
    43441 ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt
    43501 caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg
    43561 aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc
    43621 aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt
    43681 cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc
    43741 ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga
    43801 ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt
    43861 gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat
    43921 tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg
    43981 gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg
    44041 tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat
    44101 ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc
    44161 aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc
    44221 tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt
    44281 cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc
    44341 ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat
    44401 tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt
    44461 ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg
    44521 ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag
    44581 tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa
    44641 gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt tgaagacttc
    44701 tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat
    44761 atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa
    44821 gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat
    44881 gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc
    44941 acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca
    45001 tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc
    45061 ttccattgtc aagtagtagt tacctagaac catatcttgg gaaggagtaa caactggttt
    45121 accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg
    45181 tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata
    45241 agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa
    45301 tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt
    45361 gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc
    45421 atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa
    45481 taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac
    45541 aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg
    45601 accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt
    45661 aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg
    45721 tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt
    45781 attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc
    45841 ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat
    45901 ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc
    45961 acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc
    46021 atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa
    46081 ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt
    46141 ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc
    46201 caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta
    46261 ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt
    46321 cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt
    46381 gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa
    46441 cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc
    46501 gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt
    46561 atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct
    46621 gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc
    46681 aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa
    46741 ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc
    46801 gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat
    46861 gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg
    46921 ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc
    46981 atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc
    47041 ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg
    47101 ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg
    47161 agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt
    47221 acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc
    47281 ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg
    47341 acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac
    47401 ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc
    47461 ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc
    47521 ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga
    47581 caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc
    47641 atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg
    47701 caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag
    47761 caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt
    47821 taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt
    47881 atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac
    47941 caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc
    48001 aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag
    48061 agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt
    48121 tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg
    48181 gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt tacataacaa
    48241 agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg
    48301 aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga
    48361 gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga
    48421 tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc
    48481 cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac
    48541 gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt
    48601 ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata
    48661 actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga tcggacacat
    48721 acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa
    48781 tccgccaggt cctaaggctg aaagacgacg tttgtatgtc aattcgccaa gagggtttgt
    48841 ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac
    48901 tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct
    48961 ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc
    49021 accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc
    49081 ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt
    49141 tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg
    49201 tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg
    49261 atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg
    49321 gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt
    49381 ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta
    49441 acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt
    49501 acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa
    49561 accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg
    49621 taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc
    49681 acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc
    49741 ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa
    49801 tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa
    49861 ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa
    49921 tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa
    49981 acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac
    50041 tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa
    50101 gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg
    50161 gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc
    50221 gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac
    50281 ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcaaata
    50341 ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata
    50401 gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc
    50461 gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat
    50521 actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac
    50581 attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta attgacagac
    50641 ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca
    50701 acatcaacca aatcgcacca acacaactga ataactttta ctttagctac tttagatagg
    50761 tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa
    50821 gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa
    50881 atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc
    50941 atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat
    51001 gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc
    51061 acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact
    51121 tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga
    51181 agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga
    51241 caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat
    51301 cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac
    51361 agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa
    51421 tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat
    51481 cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat
    51541 ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac
    51601 agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg
    51661 aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc
    51721 aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct
    51781 agcaacagta ggaatcgact acgaaagctt gggtaaacaa acagggatca tggcagcaaa
    51841 aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact
    51901 gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc
    51961 tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca
    52021 tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg
    52081 gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta
    52141 agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt agggggtgcg
    52201 gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca
    52261 gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac
    52321 atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa
    52381 gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt
    52441 ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg
    52501 agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat
    52561 ggatgcattg ctctttctgg ctccttgctt gcgcaaaaca acggctttgc tgatttaaat
    52621 tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta
    52681 cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt
    52741 tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct
    52801 gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct
    52861 caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc
    52921 atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct
    52981 tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc
    53041 tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa
    53101 atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc
    53161 ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac
    53221 gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag
    53281 accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc
    53341 tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac
    53401 tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg
    53461 aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca
    53521 tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg
    53581 tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca
    53641 aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa
    53701 gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt
    53761 ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga
    53821 tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat
    53881 tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg gaagtttccc
    53941 ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg
    54001 ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag
    54061 agctacggtc gtttattcct gatttgaaag tacaaacgat gttgacttct gaatctacga
    54121 tgaaagatgt caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg
    54181 atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag
    54241 gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata
    54301 cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt
    54361 cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg
    54421 aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta
    54481 atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct
    54541 ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat
    54601 tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg
    54661 tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag
    54721 aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt
    54781 ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac
    54841 acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta
    54901 tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg
    54961 gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca
    55021 aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa
    55081 aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc
    55141 tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc
    55201 aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac
    55261 ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc
    55321 tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag
    55381 actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc
    55441 ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg
    55501 agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg
    55561 aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc
    55621 ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt
    55681 atcctactta ctttttccta attctaggat acctacttat atgttactac acacccttat
    55741 tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg
    55801 tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata
    55861 aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat
    55921 gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc
    55981 tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa
    56041 agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag
    56101 tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat
    56161 agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt
    56221 gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc
    56281 tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca
    56341 agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag atgattttta
    56401 taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt
    56461 agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc
    56521 agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt
    56581 aaatatcgat gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga
    56641 tcaagtagtt gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat
    56701 caaatacaat gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg
    56761 gaattttgga aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct
    56821 tgcgcctaat gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa
    56881 tgtgatttta gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa
    56941 tggtacagat acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc
    57001 agattcatca
//
LOCUS       DANKAY010000005        55933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000005 DANKAY010000000
VERSION     DANKAY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..394
                     /locus_tag="PWY98_000300"
     CDS             <1..394
                     /locus_tag="PWY98_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036087.1"
                     /translation="NGNRTVDVRENVQSSGTSQPRTVDVRENLQNTTSSQNGNRTVDV
                     RENVQSSASNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQEVKEKKR
                     PRFTYENNPLFQDISDKPNPLFDNLFKK"
     gene            445..753
                     /locus_tag="PWY98_000301"
     CDS             445..753
                     /locus_tag="PWY98_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036088.1"
                     /translation="MYRNPKNIKQEIKLFFFYLVDLGIVAGCVMCSVYFAKLMPVSPL
                     MKFAFYVLAFIVGVWMCVRTPNHPKERNILMVYYLLRMDRNRYHEVEFKRNKRGKIEV
                     "
     gene            750..1367
                     /locus_tag="PWY98_000302"
     CDS             750..1367
                     /locus_tag="PWY98_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036089.1"
                     /translation="MIIAEPNENKLAGLFDKLLDLVVPKDKDMCELLPYKKVLKDGIL
                     VTNDDKFQVLFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETIEQ
                     GIYWKNRIQEAKRELINNSDPYLEPYLKWNLKLAYDNYERVELVSTLPELMFVIAIYG
                     KTIKEVKNHADVMKQAGGRELDLQRINEEDLTNVITKLMNPNSSL"
     gene            1380..1835
                     /locus_tag="PWY98_000303"
     CDS             1380..1835
                     /locus_tag="PWY98_000303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036090.1"
                     /translation="MLKKSRKGLKHCIKENGETRFYEWKEPNLIRVLGRFDTTSSDLL
                     ETCRNNKSLIVTKDTAKYRGIRCIEKESVGVHDIKMIEDILEIYPDYSLVVYDETVKP
                     VNQCEFYKALVKITKRFENVIFIWTSKISTNKQSEKTFFSDKNVFLRSI"
     gene            1849..3804
                     /locus_tag="PWY98_000304"
     CDS             1849..3804
                     /locus_tag="PWY98_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HDL2036091.1"
                     /translation="MIVNRSVARKLDGEGYDLRFVEQYQPKGGIKFDERYITVGDGYL
                     SSVMVIEFAEDVNSRWLTDIMAIPDTIATFDVCTADKEDLLHKINTSIGELDYRSSNE
                     NRTTDKTDALLDKYNLEEFAQEITKRGEVVKTVHARIFVYNDSLEELEKKITEIRKDL
                     ASVNHKAIVPQFRMKDEWLSLFQSGVDQEAQLFAKKGLTVPSRNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFFSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVRQAGGVYVDLGGGDMVVNPLQVYATVMKKDQYGNDVIDEAKSFQ
                     NHITKCSNQIRFLEPGIDTATVREYTLMLQDFYIQRGILPQEWREAKDDNERNSLGTN
                     ICNLPNEAYPVYGEFLNYIEQQQFNIQGATPERVRSIELIKMIVKDMCTTYPQIFNGI
                     TTMPNFDTTKIVYYNIDSISKMDKPVFHCQLFTALTTIWNSALRNGKIQKENFKKHGS
                     DYFKYAQDFMVLIDECHNVINSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPK
                     DSGSEVADIIRNIFNLCTFKCFFNLDSALLNDIKKVLGNTITDTEIMLLPELEVGQAV
                     VQTSSEDTYLINFDPYPEQIERFDGGQ"
     gene            3824..4975
                     /locus_tag="PWY98_000305"
     CDS             3824..4975
                     /locus_tag="PWY98_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2036092.1"
                     /translation="MDEKQNNSLVSSLAKHGIKKLSKKFGIVLKIKILIIIAVTLLTC
                     LTLGGVLIGTLDSFQTTSTRSSGSSAEIIGGNKPLSPEVLKWQKQVEKYAERYGISPL
                     VPYALAIMQVESGGNLPDLMQSSESAGLPVNTLKYEESIDQGLKHLSNVYNIAKSYGM
                     ENNFDGICQAYNYGVAYMHYLGKNKKEHTLETAEVYSKNVVAPSLGNTTGQTYSYVNE
                     VSIKYGKTYLYLNGGNFYYAELVKQFIGGSGSGAPANPSEFWKDMKVEMEKYQGWPYV
                     WGGKNPTTGFDCSGLTAYLYQVCAGITIDSYTVSQYNASERVSIKDAKAGDLIFFRGT
                     YGAPDFISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQQHSPEIRRVKK"
     gene            4993..5643
                     /locus_tag="PWY98_000306"
     CDS             4993..5643
                     /locus_tag="PWY98_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2036093.1"
                     /translation="MINYTSTTKWKIMTLILISVVIFGGLFAMLNNKNKHDENKKLET
                     KIAELETKNEKLSSNINDLNASIQSATSSPKGEELSKEITDVASKFVELYPYYDIEKV
                     EDKKNNLLEIASKEVADSIVPEDMISSSKKTLETNSEDQKGEAYSSDPTFRSRYESAT
                     LYTNFVNAKQVQYFAEVNYETESSSGDTNNTVFIQFEVTNDNGKVQVSDYEIKYLK"
     gene            5659..6555
                     /locus_tag="PWY98_000307"
     CDS             5659..6555
                     /locus_tag="PWY98_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036094.1"
                     /translation="MKKRYSIHDNKNKNKKDKSQIDKVYRKTKGTKLYLGAIISFLSI
                     ALLIVLSPLVLGKSYDYQTVDLNNPMNISSSLNMYIDDMEINKEDGLFKLVLRYQDDT
                     KTKSLSNIKSDYKLNYITNKGNDSAKMKVVKLSDEYTVVYYQGLPKDFGVISVTIKPR
                     YVYPELEPSDDLKDKEIKFYAVDQDIKQNNKLIVESMSDLKKDNFTYQTKAIRAEIEL
                     ERDKINKLKLANEVVEKDIAKAEQELDFQTLEEQTDTKNEINSMKTTIENNKSQIEES
                     NKKINGYEEKINQLEKTASSFE"
     gene            6992..7279
                     /locus_tag="PWY98_000308"
     CDS             6992..7279
                     /locus_tag="PWY98_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036095.1"
                     /translation="MEVKIRGVDPQYIQEIDRKVKEIKRKTNRNFSRNDYFKMLIRQD
                     SEIDLINYKKAEFDLALDKMLVSNEELTASIVNLTEVYERLFNLLLSEGGR"
     gene            7282..7545
                     /locus_tag="PWY98_000309"
                     /pseudo
     CDS             7282..7545
                     /locus_tag="PWY98_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7682..8269
                     /locus_tag="PWY98_000310"
     CDS             7682..8269
                     /locus_tag="PWY98_000310"
                     /inference="COORDINATES: protein motif:HMM:NF019976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDL2036096.1"
                     /translation="MSEMNWFLLLLGGMSLLVLIAIIIGEWGVEKSSKSNLQIAKKIK
                     KKCRYAKILIDIELPDYKKMWGGRSKVKVDLIVIHGVNVYVLHFVNVEGYVYGHEATK
                     IWWCVHKSKIKFMNPMEISYMESEIVKGIISEGTEIIPIVVFPDGTNLNYINVIANDI
                     KIIYESQVVEYICSKKANVSKRNTQKLYTILQNAN"
     gene            8332..9852
                     /locus_tag="PWY98_000311"
     CDS             8332..9852
                     /locus_tag="PWY98_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036097.1"
                     /translation="MAEHFFNRKSASIILKAKFETGKSSKKNHMVELQKFVDYIARQE
                     AIRKDNIDHEYSKDELDELSRIEKALSIMEKESDQPVIKDIMQMDKYIDYMTRKKAIL
                     ENKDNEIVNGAFSNSKRYITQKDVKKIKESVIEAKNNDSVMFQDVISFDNDFLEREGY
                     YDPKTNQLNENILYEATKSMMEKLKEKEGLVDPFWFATIHRNTEHIHIHVTSMERQNT
                     REMMEYEGVLQARGKRKQSTLDDMIFKFGNKMIDRTNEFEKISKLRKDVPLEIKESVR
                     NTLLQVCIDNNSNYNTELEKYLAELKKEIPSNTRGYNELSKETKEKIDHVTDYLTKDN
                     PKRHEYDNMTRTIDQLYQETYGKRYTPEKFYEDRRVDLNARMGNAVVSQIKKIKQSEE
                     IGISGEELKKHLNNNALRLQVKHNDIDLKFKTEAGRYRYEKLVEKQSKSKEEQEKRIQ
                     NYKKRQMKFQNKKDLRQIERAINDDLHMYRAKKDFEQAQKMVEQEKIRQAYEFDFN"
     gene            9991..10239
                     /locus_tag="PWY98_000312"
     CDS             9991..10239
                     /locus_tag="PWY98_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2036098.1"
                     /translation="METITPTNLRKDMFKVLKEVANSNSEVEITMNSKNGLNDGIIMV
                     SKREWDSLQEELYLQRTGTLDHVFNLMENENDDDFEVV"
     gene            10241..10528
                     /locus_tag="PWY98_000313"
     CDS             10241..10528
                     /locus_tag="PWY98_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2036099.1"
                     /translation="MVSDWQVKVHKDVLKKQIPLLRSVGLISDYEDIIDVLKKNPYEN
                     IRSREKLNPRNKQIFSMRINSKHRVVYTIEKEKHLVKVWSAWSHYEKGIPK"
     gene            10606..11616
                     /locus_tag="PWY98_000314"
     CDS             10606..11616
                     /locus_tag="PWY98_000314"
                     /inference="COORDINATES: protein motif:HMM:NF022432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDL2036100.1"
                     /translation="MNGKKYAKIVKKVKGLLAIAKDENKDEESQSAFLMAQRLMIQYD
                     IDKADVLEEGEEAEPIGEESVTVYKKLFWWERTLGQIIAKNFRVKMFYNSKRKNGDQR
                     KKSKVVFYGFGKDLELAKEMYILAYEVLLYHSQKYINRWYEDEGIARERYLTESIKSS
                     YIRGFLGGLEARFEEQVAILTKRYEVMVLIPKEVEESYKDYSKEFGVYNSKIPPVTVG
                     NAYSRGYEEAKKVDFTQKTVGTDYSSLVDRYIRFNEGGTRGLIAEVISISENQLNLLI
                     MNTVSSGFSTSNPCFYSWVIHAEHDFELLHLTDDSVRLFIDYKNNNNYKKQIAELITE
                     LR"
     gene            11762..12601
                     /locus_tag="PWY98_000315"
     CDS             11762..12601
                     /locus_tag="PWY98_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036101.1"
                     /translation="MKLKSLGFLGLFVGCVIGLTACSQAESSKKSSDKEIAFAKVAKT
                     DEQNLWFLIEGDQTAKTPIEQDAKIDSVIVTKNGKYEMFKTEVGNLSLSDVSTMSDDE
                     IIENAEKYDQEKFENKLQSDTNDYESILQSAKEELTTEQGREFSDKSKIKELEEKIPL
                     LESDMAKLKSIEYQAPKWKEISVEINKGNDGEKAKNEIISLSYNYLSSPIWSFSNFKE
                     LSSEGEKVELIDNSKKLELAVPVAFAEVNKKIFTGYTNKNMETILVKQVHNKDQKMVL
                     DEE"
     gene            12745..15129
                     /locus_tag="PWY98_000316"
     CDS             12745..15129
                     /locus_tag="PWY98_000316"
                     /inference="COORDINATES: protein motif:HMM:NF024986.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2036102.1"
                     /translation="MAYRKGVWQPKSKEEKQKELDELIELSNKKIEEYRADPKDMIEF
                     AKFMSKIHNYSPLNLSLIDEQFEGAIAVGSYKMWSEQGFSVRKGEKGIGIYTHAPVTI
                     FIDEEGKEKTLREATAEEKKKIKSGELSSRRIEHFKKGYVFDVSQTTATEEDLPKIFP
                     NRVWNFKVDEETNLDQLERGVDTLANELGISIKDMRESMVGELGSARGAYFQYVDGTE
                     EIALNFRNSRTQNIATSIHELAHKKMHNRLEKGASYPTEIKEFQAELTSFVVCNNYGI
                     DTSDFTIPYVANWTKNNEKINSKEFRSILGDIKSTALEFIESLDRSIIEQREIMSVEK
                     DYEKKTSNEAQSALKNSFANDGKEVEKKNKIFENQVFDRDSYIFFKNGNFDGYRFVDC
                     TFDKVNMSNSSFKNTVFEKCNFEFANMENNDFTEMSMYDSTIHGGSMRNSNLKNSTIW
                     GTKIDYFNMSNVNLLSTTFEDVDFSKIKIGQSIQNLDCANFYLSGATAEESKVYQQIN
                     LEKLNRINFNSEFNPDTIKLPFREYFSDPECPEREILRSYFEGMIEFYKEKNPQTEIK
                     VVYREYDVTAKIGKNYATYMSDVKQFDICDMYGVTAENTSIDENDHLILDRTPENCDY
                     VEAFIHETIRAESLEKQQIQKSEVVATEGDVYHFQKSELPTPLKVIATEEAVYNLSDS
                     AKQYYDLKEWSEEFTAQSEIDNVEQLLSCNEAEEFYEKHKQEIENKVDDFEKATGIDF
                     RSQLSESNYQSQVATLAYKIVAVELKNEIEESQFEIPLNSKLKKEELSLSYEME"
     gene            15192..16553
                     /locus_tag="PWY98_000317"
     CDS             15192..16553
                     /locus_tag="PWY98_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036103.1"
                     /translation="MSKFDHFSLEYKGLNGTADGFGIYLTPNKDMASMYAEQTKKLGY
                     IYEVTADLEKPLSTDELTITDKELSKIIDILQESNNILNDFNDVDYYGEKVVKREAIA
                     MLKENDNDVDLINEIANTTGDTEKTAEALYNVGHYTHIVANERLLQEDTVVVVLQPER
                     INIERVQDLNKELDVPAITENSHSGDKEKLSNQEEAVRLFWKGVLEQNRSFDFVGVDS
                     NGTTLHSGTFVPDPQDRADSWLMYFDNQGEMIKQYIMSIDEIEEFKAGKKDEFMSQFP
                     HGAPNIDYKILFHEENEFYWDWDEDIKGIKVPEVPIKDVIIKDFEQELETDRYYDKQA
                     WQERIEYLNSNDPSAILRFSLDEDVEEFYKSHSDEIEDKLSSMAEVLGVERNALINES
                     EATSEVDYKRYASIMAYKIGMDEINNDIENGTFKIPAMSEETENRILTVRNMESKQTF
                     EME"
     gene            16851..17867
                     /locus_tag="PWY98_000318"
     CDS             16851..17867
                     /locus_tag="PWY98_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HDL2036104.1"
                     /translation="MKISELKKIKIVDFCAENGIELTQDSREYYRLAEHDSCVISDRK
                     NLFKWNSRNIGGDIFNFIQEYYDCSFKEAKEKLLNSDYDIRENDNQYIKEPFTYNVSN
                     EQTVFKSRRYLINERRISKELIDDLITQGLIREDKRSNVLFIWKKNEEIVGCTEQGTT
                     AKFNQKKQKNITWKKIQENSEVNYGFKLTYGKPKSIYFFESEIDMLSYITQKPDKAQN
                     ATFISMNGLKKMTILQAVTDHFNEFKRLPESVVTCVDNDEAGRTFFDTEFKGKGIGRT
                     GIGVTEVKADFPKRNGFDWNDNLTKGKEIIQKMNDHDISKLKVFCEIKFSKTVQNNYE
                     VSNL"
     gene            17889..18248
                     /locus_tag="PWY98_000319"
     CDS             17889..18248
                     /locus_tag="PWY98_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036105.1"
                     /translation="MAETIAILSDSLLDLDKKSTDYMIIEEQLKNGNPEFNETEKALY
                     IELSKEKAFKLISILFEIIERYGLTYYQVWKDKNEKGEYTKGISVEAIRDKYSISIRA
                     VKPLLGSIADWGPILNV"
     gene            18397..18567
                     /locus_tag="PWY98_000320"
     CDS             18397..18567
                     /locus_tag="PWY98_000320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036106.1"
                     /translation="MSNKIRIGIMIRGIEVRSKEEVEGVVLNVYDHIVIVFPKQGSPA
                     VVRKKDISAIFN"
     gene            18704..18895
                     /locus_tag="PWY98_000321"
     CDS             18704..18895
                     /locus_tag="PWY98_000321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036107.1"
                     /translation="MSEETRELKEIYGKIKRMSIDDIHEALKTAETEEERELYLNMTS
                     FIMQMEQKKILKRKEKVHG"
     gene            18888..19835
                     /locus_tag="PWY98_000322"
     CDS             18888..19835
                     /locus_tag="PWY98_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2036108.1"
                     /translation="MDDNLHYVVVGGVNGAGKSTIYEFDTIRIEGLKTDRINADEILQ
                     RNGGNWKNTTDVAKSMRQVVKDIKQHFEDRKSFHHETTFAGNTRPHEKRLFMAKSLGY
                     NTSLIYVGLESPELAIDRVKRRVQEGGHGVDDGIVMDRYYKSLENLSKLEKYFDNVTL
                     FDNSRLFQEIYVKKDHVVVANRSQIYNWVPETIKEKNVEKSIEQEIITSYTNKNKGYS
                     ELEKDYSSELGTLKIFQDKEYLDGFYNRHKNEISERVLGLSISIGTSSENLLGDNPKQ
                     KATELAYKMCLGNFLEKFEEKLLKTKNVDRVSEWDREIE"
     gene            20014..20331
                     /locus_tag="PWY98_000323"
     CDS             20014..20331
                     /locus_tag="PWY98_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036109.1"
                     /translation="MKNYASRKFIHLKPIKELSMNGNEYNDYKSLFVKDTPIGGIRMK
                     NMPDFRLIEVNNLGIVEYYLERLDEAVNLSIQQNGEEHILTVVLKDLKNRLESEAEFF
                     YED"
     gene            20494..21057
                     /locus_tag="PWY98_000324"
     CDS             20494..21057
                     /locus_tag="PWY98_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2036110.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYESKFHKRSD
                     CMKPNLIETGKRIKSIRSNLGMTMEQFAILTDSSNTSAVNNWERGYNLPNKTKLKKIA
                     ILGNTTTDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSKEFEVNQIP"
     gene            21071..21946
                     /locus_tag="PWY98_000325"
     CDS             21071..21946
                     /locus_tag="PWY98_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2036111.1"
                     /translation="MKPNPVETGKRIKSIRFNFGMTMEQFADLIGNAQKGNINNWERG
                     FSLPNKERLIKIAELSNKTIDWIKWGTLEEYIASYLKDSGYELYIKDFPEIPHKVFND
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDLGLTDDNKNVKMI
                     GQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAEIGGVSEL
                     YLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKIDDPDKREI
                     SLNAICQLSLLDKMG"
     gene            21995..23377
                     /locus_tag="PWY98_000326"
     CDS             21995..23377
                     /locus_tag="PWY98_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036112.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRSNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLEE
                     YITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDKDYELLDSIIKNIFTKYY
                     SKEFEVTQIP"
     gene            23391..24173
                     /locus_tag="PWY98_000327"
     CDS             23391..24173
                     /locus_tag="PWY98_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036113.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            24213..24821
                     /locus_tag="PWY98_000328"
     CDS             24213..24821
                     /locus_tag="PWY98_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2036114.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLAFVREGDTLVFESLERLGRNSDEILDTVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVISIFSWTAQNEREEIKRKQKQGIAIAKKKGIYKGKPYEYSSTAKNPQ
                     KRFIYSEIVRMLGEGVPIKQIAEKTGTNRTLVYRIKNRILKE"
     gene            24827..25099
                     /locus_tag="PWY98_000329"
     CDS             24827..25099
                     /locus_tag="PWY98_000329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036115.1"
                     /translation="MNEMLFILVANSFVLLIILTCDFILINIGVHSFIVNLLLFVFSL
                     IQVISFWILWKENLLNTIYVLIAIMPLLYLLAGIIGIARNRDNFFL"
     gene            25144..25482
                     /locus_tag="PWY98_000330"
     CDS             25144..25482
                     /locus_tag="PWY98_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036116.1"
                     /translation="MSIENAEITGTLLGYGEHDILTFSIGLKGNGWGVNYGSRALDGY
                     IKKEDERKPNQAAMSVIPEILKVVGVRKWEDLKGKYVRVETNGLGRTVTKMGNLIDDV
                     WLDLDDFFNT"
     gene            25648..25965
                     /locus_tag="PWY98_000331"
     CDS             25648..25965
                     /locus_tag="PWY98_000331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036117.1"
                     /translation="MWGVIYLVLYVIGKLTDLIYFNQAICFIIFILVIIIINACIFRS
                     GRNKKRRIDKTVLLNLIVIIIGVGTFTSKALISSVILLIFDFLIIYSVLYFINKAREY
                     LNQ"
     gene            26062..26634
                     /locus_tag="PWY98_000332"
     CDS             26062..26634
                     /locus_tag="PWY98_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036118.1"
                     /translation="MTAIFKARKNSNVSEYVKARLQKNNLEYILENGVGNLLNELTED
                     IDTDYIVLWDNGTLEVSIYGDFYQTHSKAMFKKSLSGRSDIKGIRKNSRFYKENQEVF
                     SKLAEIIPSMVAPISKRLSKKYFGTVYDGFGLKSNVEKYYYFDDTAYLAIKDMKRIPT
                     ETLDQLEQMKLSEFYSIYEEVVKVGATTNG"
     gene            26627..27466
                     /locus_tag="PWY98_000333"
     CDS             26627..27466
                     /locus_tag="PWY98_000333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDL2036119.1"
                     /translation="MDNQKVGNKKSKKNDLKKELINFRQSLKEREYNLIFRLDVWRAL
                     KVGSGFLGYMMWLYLARIGWINLYSIFRVFVEDESVKVGINTFGNLMFNLLLIALIVL
                     QSVKIPLTKELSGYTSILKPFQEGLKIICKQGTVVFLIGIFTDEINFIDYKNVIIRLC
                     SFLLVYLIATTFEKVTFRKIETLVQKEISKEEFCLETPYGKLQNVSLSTFCLNSKWEK
                     YLYRSERRVSWGSYSPILHECLRETGAVADGEIMVLKDVFGDYEIAFLDQPELRSDCS
                     ANG"
     gene            27972..28226
                     /locus_tag="PWY98_000334"
     CDS             27972..28226
                     /locus_tag="PWY98_000334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036120.1"
                     /translation="MELTKGKRIAVVRFDSLSINDSRYIMPVTDVVFKTEDGIRLIWR
                     LPIKKSERLLKKIKVLEIEFVVTEKIAPETYLIEQVKIFG"
     gene            28661..30259
                     /locus_tag="PWY98_000335"
     CDS             28661..30259
                     /locus_tag="PWY98_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2036121.1"
                     /translation="MLDRQAYMDVLEGILKDGLQKFNSKNVMPLNKSVNKKGAIVGFE
                     SKGRLIQSQGIIYRSLEKLYADSELLTHWTPNVFSWIGGGKGQSVWGHREDNLIQINT
                     FVADIDFLDKKDKISLNSLVLYLLEEGLLPYLILDTPKGYHAYFLIKNYNEESREYDK
                     ASYISNANGYKSLHVAKRISENIRKAIKNRLPEVDMGCNHFGVFRFPTPRNIVHFEPN
                     FTNTFEGYLEWSKEFERKEKEKKKGNMTVLSNKTRIKSNFRQIDTKWYDYLIHADIEE
                     GDRNSVIFTLSLACKQSGLSMEQCIDQMDQIEFKNDLSNKEVQRTIKSAYEGAYSGAK
                     GFYINELLEKYTTPSQFKKYAVATENKMVYKRKGNRVDPCYWVKFAKNREERTYSHFS
                     ESQDDLMAYLKSKEKQLNENQLYLELNMVNLSEETKIPLSTLKVIIKELKQDKDIIVK
                     TKRGRNGSTKIATRRFVEVKVLMAVISRNKGNKLFATELLEKDEQTLVEEIISRYENK
                     ALPFSVDKEKVQREKIAPIRGATG"
     gene            30516..31352
                     /locus_tag="PWY98_000336"
     CDS             30516..31352
                     /locus_tag="PWY98_000336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036122.1"
                     /translation="MFTSADEKVIEQKEPDRNGTINGIKRIVCNRCNGSGSFKYVSMG
                     RLTCGVCFKCNGLGYVDKKFKIMTPEYRAKRDKINLCNQQKAEEKRIAKELERLYWQI
                     KQKGFDQQIIYIVNEPDTYVIKDDLKSAGALYDDVIGWYFLNAVKHFSTEMVLTQDIY
                     EIKNSELIYKQTYLDKSIQTEGEFLFSVGDSFELVVTYSKYERIDNPFAYKAYIYFYE
                     FLDESGNLFIWKTSKNEIDVVQGEKICIKARLKDHKEWKKRNKVIRQNMIKNVRIIKD
                     YK"
     gene            31440..31802
                     /locus_tag="PWY98_000337"
     CDS             31440..31802
                     /locus_tag="PWY98_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HDL2036123.1"
                     /translation="MNSDGIFNNADFRKGDITVNRSDYVFPEESGVFVIEGVTVCRYW
                     GKRKNLILLLDSLDGRKLKFSFWSNTKYCCEPSRSREVDVEKESVRYLKDGSSIILTC
                     EKSKDAKITRIRNIEIKK"
     gene            31872..33986
                     /locus_tag="PWY98_000338"
     CDS             31872..33986
                     /locus_tag="PWY98_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HDL2036124.1"
                     /translation="MGTVILAEKPDQGKKFATALAGKTPVNKGGKYEFESEVFGHTIV
                     TWGIGHLVGLSLPEKYEWLPNKEKWDLANLPFLPKENELRYEVSKGKSQQYSTVKSCL
                     ENADMIIIATDPDREGENIAYNIFKLCKKKIFERPIKRLWINSLVKTEILKGFKNLRE
                     AEETVDLFKEANARQIADYLVGMNYTELFTLKLQEQGLRGVVSLGRVQTPVNTLVVEN
                     DQAIKNFKSEKYKVIECQTKNKEPKTIFRNSHEYFNMDELEVDTKKFGLDSASTGIIK
                     SIEVTQKQTEPQKLFSLGGIQEYANTKWKYSSKKTNKVIQDLYDQGYLSYPRTDSELI
                     TTNEFEYLKNNLHRYKELLKLNINTPCVQPNKRFVNNDKVLEHYALIPTMEIPDIAKF
                     KTEEKNIYEIIVKRTCLMFAEPYIYENTKVVLDVNGYEFMANGNVPVSLGWKQVDQNI
                     DEKKDGDGEGVSLPKFIEGEVVNIKVNFIEKNTKEPKRLTEGKLVGKTGIMAKLGLGT
                     PATRAGIIETLLAREYIRIENTKVYPTPKGYLLWDLTKNKDLLIGKPEITAQWEQALS
                     KIAKKQYTREEFLKNIYKYLDVTIQDLKKSDFESLYLEKSTIQNKYEINGYVVEEKAK
                     VFEVVKSETKEKFVIFKNISNKTITKTIVEELLKNGRTKKPLTGFKSKKNKKFSAILV
                     FDDLEFKVKMEFENKKADSNKF"
     gene            34114..34683
                     /locus_tag="PWY98_000339"
     CDS             34114..34683
                     /locus_tag="PWY98_000339"
                     /inference="COORDINATES: protein motif:HMM:NF016214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2036125.1"
                     /translation="MLYKNHLLVSNAIAIPFMGMTNTLTVSSIVALSLGALLPDIDEP
                     ESYVGKRTRGLSDLINIFFGHRGITHSLAFVLFIGLLIVPLTSKIGFVSLGVWLTVGV
                     FLHIFEDSFSNSGVNWLLPISEKTYHLNIYSTGSLIEFLIGLVALIWIVVCFKGQYFV
                     LSQDELVNVAVLKNMFIDIKDTLSKILLE"
     gene            34707..35306
                     /locus_tag="PWY98_000340"
     CDS             34707..35306
                     /locus_tag="PWY98_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HDL2036126.1"
                     /translation="MNNSPPKNPKDKAKLFTIENEYINKLKSIDSRVQNNYKGQRLYY
                     KSDLEVGEGINYYVPLSSAKESQKKITNKSVFKLYGDKAQEDFLGVLHINNLIPVPDQ
                     YAKIWSPLAKTDERYLTLVIKQARYIKAHEEEINKDCKLMVDSNMGTLDPVYFSKNRK
                     DIMTYKRITSDLTKLEQVSKAERNTSDSVRHRSNDIEIN"
     gene            35412..35726
                     /locus_tag="PWY98_000341"
     CDS             35412..35726
                     /locus_tag="PWY98_000341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036127.1"
                     /translation="MSFLEKYKAEVLDELEKEYKEVSVDYIQSDLLDDSLSEVEFVKQ
                     TLEKIILSEERPLLTLKQLFEKYHVLQNTYDTAKYVFHLSTENLDENIVEKAIDYLAI
                     EF"
     gene            35776..36000
                     /locus_tag="PWY98_000342"
     CDS             35776..36000
                     /locus_tag="PWY98_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036128.1"
                     /translation="MARPKKYKTEEERKKAREKSKKKWASRNKDKVAYIRAKSSAKLF
                     VEMSEDEDVEMLERWIEERKKRGKISDNND"
     gene            36249..36455
                     /locus_tag="PWY98_000343"
     CDS             36249..36455
                     /locus_tag="PWY98_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036129.1"
                     /translation="MIENRVLGFIECEDDINDTLEVLESIVFNTKVLDLKAVNNDMVT
                     HIIVDHEKAAQLGEILIGWANGEL"
     gene            36553..37398
                     /locus_tag="PWY98_000344"
     CDS             36553..37398
                     /locus_tag="PWY98_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2036130.1"
                     /translation="MKKLNLGYVMAINMFKGGVGKSTLTQLMADILSAMGFKVLVIDV
                     DPQGTVTKKFQRQYDLDVIGRMTLFDAVINMSFMESITSIKENLDIVIGSEQMSRFTK
                     VLRDMWRSTGFSDEGEEIFWNLFNYMINNDDLRSQYDFVLFDTIPTVSEFTDNVYVAS
                     DFLVIPTQTEQDSIDNLHSTVNNYRTAVKEVNSNLEILGIVPYLVDTRLKTGKELLTE
                     LQEEYGDMVYSNVVNDKGVVRRWGREGLDKSIPHSKKTYQMYVNIAVETLERLEKVTG
                     ENLLN"
     gene            37415..37852
                     /locus_tag="PWY98_000345"
     CDS             37415..37852
                     /locus_tag="PWY98_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036131.1"
                     /translation="MMGKTKKVTSAGGNNSGNPFGIKSGSGGFNIGQQMLKGKAGVKS
                     AGDKRKSVRVPIQLYNEVVARLSHGEKYPLMTEILTSFLKEFEQKGDSIIQNFNDLET
                     RPIKISEEAFRILKVFKFETDQSNTDIIASALTWYKEKNKGKA"
     gene            37876..38175
                     /locus_tag="PWY98_000346"
     CDS             37876..38175
                     /locus_tag="PWY98_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036132.1"
                     /translation="MNRDQYSHKEDKYVWENILILAGIVLGTILLAYFIVIPNLFTFI
                     NWFVPNFWSIFGYIIILWIAYKILSFIWFPVYSILGKWSYLILLATLIYIFMQIA"
     gene            38177..38623
                     /locus_tag="PWY98_000347"
     CDS             38177..38623
                     /locus_tag="PWY98_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2036133.1"
                     /translation="MKVLNDIKANFGVITWYTIMLILCNITLSWIPSFFINQVNYNQG
                     ETSFSSSVLLISGIFAPFFEETFMRGIFQNWLEKNITIPLWGIYGIVAIVFSALHMQF
                     YFVPFFITSILLSYVYDRSEKKLVVPFLIHSLYNIFVIVISNIFSH"
     gene            38770..39099
                     /locus_tag="PWY98_000348"
     CDS             38770..39099
                     /locus_tag="PWY98_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036134.1"
                     /translation="MKIESAEPNLQTPRTSKIQNSKKKTKIFKSEDVVRVAWSVKTFD
                     EDRYVKVSMNGLFVQLGNFSSFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKD
                     KGIMIPA"
     gene            39473..43102
                     /locus_tag="PWY98_000349"
     CDS             39473..43102
                     /locus_tag="PWY98_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036135.1"
                     /translation="MKKIKLISVALFSLITLSTAHQSVASAEGANSEKAVVEESGDKK
                     VAIDDNVKNEAEKAAVVQGADGLMYVVVDEIDPKKLLEAESPLNNEQENTGSVEVGVD
                     KKRQEEADQANEKVKDFEDVDYNDISSLQSALNNPKFPFLSDDDLKEQLSKAKDTFES
                     EELIKKTRGYFSQRILELGNNLNDVPQRAKVNRRALVSTRASKVSETRINPPSGYPWV
                     IYLVRSNGYSYTENYAIRRTIDGKEAFCLQAGVTATNTYTGSASGYLSNSQKELISSF
                     LSAYKELGGWANNDSGKQRWIATQILVWENSNEKPKSITVTPNWKSGVTKSKSDITAR
                     ANTIRVKPTWDVNSKTVYEGEKATFTVKNTGGEHKLKVESVSGGGKATISGNKLTVDT
                     SGSTGTQIVVKMTKGSTELNPTTVWSNGSYQKLVTGSFYVNTSVTVNVKRKGTVEVLK
                     IDSETKKPLAGATFRFSYSGINKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQL
                     DSSEFSITVKENQNVTTTRTNKRSTGSVEIEKLGDLDGLGLPNVVFTIYSSDNKVVKD
                     NLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEITQDTPSTKPAKVKVTN
                     IYQVSISEKVSDMNESQVLKNVWSADPNDYLQYDITTGNLKKSGERNVQSFIIEGFYD
                     NKNVDVLKSEVYVGSTNVTNSFDISNDTGNGKVTAKAKATVLTNNDFYNKSYNLRITM
                     KIKKSTIENTEKQKPLTIVKNSKVTVGSKISKSASSNDVETVLYSRKITINHIDEKDK
                     HLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIQKGKINGKDIVLTTPYHIPAL
                     SVNVDRIQIDTSRAVKNGFLPTELELSKKEEYGNELSKIVFKVKITDIDSNKVVYDNN
                     FKYKEFSSHIKLNLPTEYLTKNKKVNYSISILLVENPEKNKFDTETKDLKTIGYTSSE
                     KVLTNKDMTNNQVNYSAVVRTVKERANNNPVKEFRESINYKFTDTTKTKTGYGISVDL
                     EPTYKNEIGKQIDMKVYSYPMNELIDSKIDEIYQPIGEKEAEVKMDRLKNESKVESGT
                     NTQILSFQFPHMNVERKTGNVYADSQKNDESIKYELIDGGRKLYVPIWCDLGKYSIAI
                     KSSNFGGNYISLDMNQSIDVYAYMYATIDSETKKEDELLLEPVYPETTLPDNWSNKEI
                     DWLNK"
     gene            43163..43531
                     /locus_tag="PWY98_000350"
     CDS             43163..43531
                     /locus_tag="PWY98_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036136.1"
                     /translation="MRVQSTGYGNSVFDVKREFQDSRRKTRKLIAYFILSAIVFYIIR
                     FVLELNSMVFVDMVHKMLNGESVDFNFAYMLDQAKSNQVVAFLISVYYLIEKSVFSLK
                     EFLLGLFSRFVDVVGKGIEN"
     gene            43547..43699
                     /locus_tag="PWY98_000351"
     CDS             43547..43699
                     /locus_tag="PWY98_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036137.1"
                     /translation="MLDQNTDRSWWMIGAVIVGAVLIGIAKVAFPEVFNTVIDFFKNM
                     IPTRLG"
     gene            43748..44167
                     /locus_tag="PWY98_000352"
                     /pseudo
     CDS             43748..44167
                     /locus_tag="PWY98_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44180..45607
                     /gene="tadA"
                     /locus_tag="PWY98_000353"
     CDS             44180..45607
                     /gene="tadA"
                     /locus_tag="PWY98_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2036138.1"
                     /translation="MRVIEQEKPSQPTKSLVNLVKAKKFIKAENNISVKDEDFLQTLL
                     EDDTANYNPPIEEARLNMIRDDLTRNYSEEVLQALTDDEIRKKLVEVVLKEHGSLFNK
                     DKSREVAEYVVRECIGTGIIERIMTNDDITDVGWNGSQLSIETNSNKWLIDGEKLNIT
                     QEYIERVISKYAVVNDRDFNTGNPILDGMFKNVRISATHIDNSPDGATMSMRVARPKL
                     ALNTTNFENFAPDYILDLFEKMVKTKANICISGETGTGKTELQKLLLSFIDNHDRMIM
                     IEDVQETHAKELFPDKDIYAWITTPKKTISDLVKACLRNNPRWIIVSETRGAEAYEML
                     QAILSGHHVITTLHAINARAIPRRFVNMCASGYNINETAVEEDINRYFDFGVHIKKVV
                     INGKVIRYLNEIVEFSDTGTTTVFKQKLINGEFIRENGALSDDFKERMAEKFISFEFP
                     EDKIEKEDSETNELNSENSEVEVIT"
     gene            45604..46434
                     /locus_tag="PWY98_000354"
     CDS             45604..46434
                     /locus_tag="PWY98_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036139.1"
                     /translation="MKVQAVTKKKGIMDVSELNAYQVAYGKPLDYRDYLNYVGLPCIF
                     MGVFSFSLCYFWWVGLICGVIGAVYGYKVVMPKSVKRSYELKSLRERNKFINNMTQIL
                     TDESKTVTKALAMAKTRTKGELREDIQILESRLQGADKFQIAEAFREINEKYERDVVF
                     TQYFEQLETAIYEGRNNADTMKQIKSYHNDVKLKTESFLKIKDGYLQDLKQMLFIVAI
                     FIGAITFSFGFSTFFDGFARSPIGWGFGGVYFIIMVRFMKSFFMHYFDDEIMSLGVSN
                     "
     gene            46434..47222
                     /locus_tag="PWY98_000355"
     CDS             46434..47222
                     /locus_tag="PWY98_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036140.1"
                     /translation="MKVANVQKKASKKARIIKQENKSIWLKMLDENYYKKLREMNNSN
                     VSIIKWQKQRFLKSWIFVGVGLVMYLLLGKEWYYLAGGIATAIGFYIIQAKSINNIYN
                     QFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKQLLMKLMQDMT
                     DRPNDIQPFVEYAEQTSGTDMSVLFMQTIYDIRQGSADLDVIEELDRLASEELMAGID
                     SIIDFKSRRFVFYPTKIAMTSFILVVGFAVGVLFLNLKDINFGQ"
     gene            47241..47603
                     /locus_tag="PWY98_000356"
     CDS             47241..47603
                     /locus_tag="PWY98_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036141.1"
                     /translation="MQEQSIMEIVKWFIGLVIIMVMVSVGLFCYQLQDVNSFKQQVNY
                     QIERRGGLTAEALKSLKEYSDKNYQGRYKIESNLLNQKVEYGQAVDYTVKATIPIQIL
                     PLPDVTLSFEGNSVSQIR"
     gene            47616..47762
                     /locus_tag="PWY98_000357"
     CDS             47616..47762
                     /locus_tag="PWY98_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036142.1"
                     /translation="MQENNTARILFLIGGMVILSLVITGIATHWEEIKSLIETFFQNK
                     MIRM"
     gene            47774..47974
                     /locus_tag="PWY98_000358"
     CDS             47774..47974
                     /locus_tag="PWY98_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036143.1"
                     /translation="MDETVEQVNKPKRQRKKKVDPFIEMTDKIIQKKGLTPREFDQTA
                     IRKERQKLYDEFVKENIGLLLD"
     gene            47994..48272
                     /locus_tag="PWY98_000359"
     CDS             47994..48272
                     /locus_tag="PWY98_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HDL2036144.1"
                     /translation="MKEIMITKLAMNMPKMDFFDNVSKFMSNTTSKLQGISLSVIIFC
                     VVVTGMMFLFGEGPSRTAKKWLTYIVIGALIIFGASTIGQTIKSVGGF"
     gene            48354..50183
                     /locus_tag="PWY98_000360"
     CDS             48354..50183
                     /locus_tag="PWY98_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036145.1"
                     /translation="MKQRFEYSFEDVESKLLRKGKGLKKKNKYSLKQAEGLIAWVQHN
                     WPNKTEVTVMCKDTEDGAVYAFDGIEITPFGMGGEPIALFPIIEDQILQAPDNMINDP
                     NKLILDINSQYQSEPDIAEAKQESKSKKGLNLPSVLPNVKLPFGKKKHLEETPESDQF
                     FEEYETESTPELDEELELDEESEEVEAEIETDLEQAEETMLDETESFDFEEEIDMTTS
                     SHESVAINSQTSVPAYSQNTYHSEQNFALASSIPQTVRQIKKHETVQLTPLQEYCDLG
                     EEIESSIVSTTNKLQPENLFKFIGIPAVKNTRLDEYRINHAMQRLAEVKFENLREHYS
                     RQVNSMIAEALDKLREAIDVAWKKPYDLEVKKRYADILEEMETNSNSTLEEFVKRQYD
                     MVDEKIKKFETEQEIALQNFIANQEAEKAVFIANEKERAQNLIDTKSETITSELEKRR
                     EEFIDEEMYKMKIDMNQNLFDGKRLIKQELAVELANANEDVWENALKFITEIQNEINE
                     KTPLWASEIKETNILEQQAHQMQKEQQELKLKEESLAIKRLEAETNLKRLEELDHENK
                     MLKIKLETANSKNELYEYEKETQQKVVGSTQARNLSTLGLFRK"
     gene            50203..50919
                     /locus_tag="PWY98_000361"
     CDS             50203..50919
                     /locus_tag="PWY98_000361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036146.1"
                     /translation="MSVFNNKNMVSGYVNHVAVYDGGSFKTMFVRLAVFNYMKEDKAQ
                     YEVINAQISCSVDQQGRLTTTSSHPKALLNKMLFDKLDKNLVEGMKVTLHGSLRGSDQ
                     VFLSGRWRNFKDLSSAEKEEYKSLPKGQLITRTNSFIYIEEVNMPSKEGFLKRKEELL
                     TENQMNEVKVTEPSQADQMETDDNDPVDNEGVVVETVEAEIIEPQEGTKISRRNTVSY
                     VRFEDMIYPEATMVEEDYPF"
     gene            50937..53792
                     /locus_tag="PWY98_000362"
     CDS             50937..53792
                     /locus_tag="PWY98_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2036147.1"
                     /translation="MRLINDKKPDNKLKVLLEKGNKIEIGDMMWFKGKNKYHSGKIEG
                     EDLPENVKDMVNLPLLLLGTVLGLFANLTVNYLINFFAAFLKALSVSEKGLMSIEFDH
                     GFHMGYLKPFTSGTQYFMISIVVCIAITFLVYNKLDHESDRNLVYGQKGDSRFATVKE
                     IQNEYHEIPEKKVRFEGMGGVPISHYKDKYYIDRDTVNTVVLGASRSGKGETFVVPTI
                     DNLSRAKEQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLQSMSFNPLQLVID
                     SWANKDYHEASKRANTLTSMLFASGMGTENEFFYKSAKNAVNAIILTIVEHCFDNNCI
                     EKITMYNVAQMLNELGSLFYTDPKNPNKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSLQFKLNTEMVGKRVKIS
                     FLRKTDGKTKIINEYRVKVKALGLCVLNFDESLRDGDFLEIRYETEKEKFRAWYSLEF
                     PKRDSEDHDTTVITKKFKSGKRFLDLEINSVVLKYTDQPTAVFMVIPDYDPSNHVIGS
                     IFISQLYTELATNCKETPSKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNILFDL
                     VIQSYSQLESRYDKGAKIIKENCQNHILIMSNDDETIEEISKKCGNKTIIGKSASSKR
                     MDTDSSVTSSADQERIITFERASQLIEGEQVILRNLHRQDKNRKKIRPFPIFNTKATN
                     MPYRYQFLNDDFDTRKDINEIDIACDHINLSLADNQIPFASFIRDLKTRMEYSITNEI
                     LISEDDYEEYRSLPNSQQTLDEEKLIGLVKKSNFEKKSEVELSEDDFKLTTYLLDCQK
                     HTKKLHDLMYEKDRFADDKSLDLVLKTLNERYFSSELVQEFQKGRVTAQNIKQSKKQL
                     AGIINVLGQVYIYAENNIFKTHIIAVKNALTKLSKAI"
     gene            53865..>55933
                     /locus_tag="PWY98_000363"
     CDS             53865..>55933
                     /locus_tag="PWY98_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036148.1"
                     /translation="MVLLLATILLFVMVPTTAYAWPWDSDDKEALDFLLKYQDYLQFT
                     KGGFGSIVMQTFSWGIIRFAYWISKTIEGLIPQALSLFSFVESAGLNKVYQSVLNTVV
                     VGLMVLSLVIIGYKMVTGKGTLDLKSVGMNVVMSVALILLMPTMISSGITFAKTFYND
                     ATSINNQDGGVAWSLIEDGVTDLVYINKNDAYNRMGKEGQKKNALKEKDFFTYDFSEV
                     ITTDTAKELEADNPKAEYLKMKLTPDGDGNMQAVKIDDGWMAIFSDSFKGGYYRYTKN
                     TFALTLGLIALAIAYVFSAFIIITAILELAFKRVLGVLVFATDLETGQRSKQVLSDIL
                     QCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVAYVCAVIVLIKGSGTVMRYF
                     GVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKNSGGNDQAEGEERKPERNFG
                     QSLSQKANKTGKTLGYARERGLSGLASDGATMASERATKPFKSMRDMANDTKNKFKEG
                     LDDGTVSAINKNSKPIVAKNKEDETGKYADSMPTRLSDRKDGAKVENADRIMSSSERM
                     REAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKSAEELINQKRQEAKYTPEAM
                     NREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGLEKSVKENLQNSTSSQDRRS
                     VDVRENL"
BASE COUNT        21370 a         6994 c        11196 g        16373 t
ORIGIN      
        1 aaatggaaat cgaacagtag atgtacgaga aaatgtacaa agtagtggga caagtcaacc
       61 gagaacggta gatgtacgag aaaacttgca gaatactaca agtagtcaaa atggaaatcg
      121 aacagtagat gtacgagaaa atgtacaaag tagtgcgtca aatcaaccaa gaacggtaga
      181 agtacgtgaa acaagagctg aagcaagtca gggagataag acgcaaaaaa taaatattat
      241 cgaggaacga aaagaaaggc ctaacgaaaa agtgattgta gaacaagaag tgaaggagaa
      301 gaaacgtcca cgatttactt atgaaaataa tccactgttt caggatattt ctgataaacc
      361 gaatccactt ttcgataatc tatttaaaaa ataaaaatga ggtcggcata gcgtgatatg
      421 tcgatctttt tttgaggtga aagaatgtat agaaatccta aaaacataaa gcaagaaata
      481 aaattgttct ttttctacct agtagattta ggtattgtag cagggtgtgt aatgtgtagt
      541 gtttattttg caaaattaat gccagtttct cctttgatga aatttgcttt ttatgtcctt
      601 gcctttattg taggtgtttg gatgtgtgtc agaacaccta atcaccccaa agaaagaaat
      661 attttgatgg tttactatct gctacgaatg gatcgcaata gataccatga agtggaattt
      721 aaaagaaata aaagggggaa aattgaagta tgatcattgc agaaccgaat gagaataaac
      781 tagcagggtt gtttgataaa cttttagatt tagttgtgcc aaaagataaa gatatgtgtg
      841 aactgttacc atataaaaaa gttctaaaag atggaatcct tgttaccaat gatgataaat
      901 ttcaggttct atttaaggtt aaaactaccg atctgacgtc aatttctgaa gatgggttta
      961 tgcgtatggt aaataaattg acctacctta atcgagtata tttagaacca tacaaaattt
     1021 attctcttac ttatcctaca gaaactattg aacaaggtat ttactggaaa aatagaattc
     1081 aagaagctaa acgtgaactg ataaataatt ctgatcctta tttagaacca tacttgaaat
     1141 ggaatttaaa attggcatat gacaattacg aaagagtaga gcttgtgtca acgttgccgg
     1201 aattgatgtt tgtaattgct atatatggaa aaacgattaa agaagtaaaa aaccacgctg
     1261 atgtgatgaa acaagctggt ggtagagaat tagatttaca acgaattaat gaagaggatt
     1321 taacgaatgt tattactaag ttaatgaatc caaattcttc attatagtgg ggtgaaaaaa
     1381 tgttgaagaa aagtcggaaa ggtttaaaac attgcataaa agaaaatgga gaaacgagat
     1441 tttatgagtg gaaagagcct aatttaatta gagtgttagg aaggttcgat acaacaagta
     1501 gcgacttgct agagacctgt cgaaataaca agtctttgat tgttacgaaa gacacagcta
     1561 aatatcgagg aataaggtgc attgaaaaag aatctgttgg tgtacatgac attaaaatga
     1621 tcgaggacat attagaaatc tatcctgatt acagtttggt tgtgtacgat gaaacagtaa
     1681 aaccggtgaa tcagtgtgaa ttttataagg cgttagtaaa aataactaaa agatttgaaa
     1741 atgtgatatt tatatggact agcaaaatat caacaaataa acaaagcgaa aagacgttct
     1801 ttagtgacaa gaacgtcttt ttacgttcta tttaaaggag agagaaacat gattgtaaac
     1861 agaagtgtag ctagaaaatt agatggtgaa ggatatgatc tgagatttgt agaacagtat
     1921 caacctaaag gcggtattaa atttgacgag cgttatatca cggttgggga cggctatctg
     1981 agttctgtta tggttattga atttgctgaa gatgtaaata gtcgatggct aactgatatt
     2041 atggcaattc ctgacactat cgcaacattt gatgtgtgta ctgctgataa agaagatttg
     2101 ctacacaaaa ttaacacgtc gattggtgag ttagattatc gatcaagtaa tgaaaataga
     2161 actacagata aaacggacgc tcttttagat aaatataatt tagaagaatt tgctcaagaa
     2221 attaccaagc gaggagaagt agttaagaca gttcatgcga gaatctttgt atataacgat
     2281 tcgcttgagg aattagaaaa gaaaatcacg gaaattcgca aagacttagc tagtgtgaac
     2341 cacaaggcta ttgttccgca attcaggatg aaagatgaat ggctttcact attccaaagt
     2401 ggggttgatc aagaagcaca attatttgct aaaaagggct taactgtccc ttctagaaat
     2461 attggtggcg gaattccctt tcaccataaa agtttaaaag acccacgagg tttttactta
     2521 ggaacgacaa gtaccggtgg ggcatttatc tttgatccat ttttttccac aaaacaaaga
     2581 cgttctttca acggttttat tcttgggaaa atgggattcg gtaaatcaac cttgattaaa
     2641 cagctacaag aagggctaca agcgaagaat tgctttataa gaggatttga taagtcgggt
     2701 gaattcgaag gcatggtacg gcaagcagga ggcgtttacg tagatttggg cggtggagat
     2761 atggttgtga atccgttgca agtatacgct acagtgatga aaaaggatca gtacggaaat
     2821 gacgtgatcg atgaagcaaa aagctttcaa aaccatatta caaagtgtag taaccaaatt
     2881 cgatttttgg agccaggaat cgatacagca acggttcgtg aatatacttt gatgttgcag
     2941 gatttttata ttcaacgagg aatcctacca caagaatgga gagaagcaaa agatgataat
     3001 gagagaaatt cattaggaac aaatatttgc aatctaccaa acgaagcata tccggtctat
     3061 ggcgaatttt tgaattatat tgaacagcaa caatttaaca ttcaaggggc tacaccagag
     3121 cgtgttcgtt caatcgagtt gattaaaatg attgtgaaag atatgtgtac cacataccca
     3181 caaatcttta atggtattac tactatgcct aattttgata ctactaaaat tgtttattac
     3241 aatattgatt caatcagcaa aatggataaa cctgtgtttc attgtcaatt attcacggca
     3301 ttaacaacta tttggaacag tgctttaaga aatgggaaaa ttcaaaaaga aaacttcaaa
     3361 aagcatggaa gtgattactt taagtatgca caagacttta tggtattaat tgatgaatgt
     3421 cacaatgtta ttaattctaa caataagtat gcttgcgagt atgtcctaag ttttgagcga
     3481 gaaatgcgta aaatgcgagc aggggtttac ttcgctacgc aaactcctga agaaattatt
     3541 ccaaaagact caggttctga agttgcggat attattcgga atatctttaa tttgtgtact
     3601 ttcaaatgtt tcttcaattt agattcagcg ctactaaatg atattaaaaa agtcttgggt
     3661 aacactatta cagacacaga aatcatgttg ctaccagaat tggaagttgg acaagccgta
     3721 gtacaaacgt caagtgaaga tacttacttg atcaattttg atccgtaccc tgaacaaatt
     3781 gaacgatttg acggtggaca ataactttgg ggaggatttg aaaatggacg agaagcaaaa
     3841 taatagccta gtatcgtcac tcgcaaagca cggcattaag aagctatcta agaaattcgg
     3901 aatagttcta aaaataaaaa ttttgataat tatcgcagtt actttgctta cctgtttaac
     3961 attaggtggc gtattaattg gaacgttaga cagttttcaa acaactagta caaggagtag
     4021 tggctctagt gcggaaatta taggtggaaa caaaccactt tctcctgaag ttttaaagtg
     4081 gcaaaaacaa gtagaaaagt acgctgaaag gtatggtatt agtccactag taccatacgc
     4141 tttagcaatt atgcaagtag aaagcggagg gaatctgcct gacttaatgc aaagtagcga
     4201 gtctgccggt ttaccggtaa atacgttgaa atatgaagaa agtattgatc aaggtctaaa
     4261 acatttaagc aacgtttaca acattgcaaa aagttatggg atggagaata actttgatgg
     4321 tatttgccaa gcgtataact acggtgtagc ttatatgcat tatctaggta agaacaagaa
     4381 agaacacaca ttagaaacag ccgaagtata ttctaaaaat gttgttgcac ctagcttagg
     4441 aaatacaaca ggtcagacat attcctacgt taatgaggtt tcaattaaat atggaaaaac
     4501 atatttatat cttaacggag ggaactttta ttatgctgaa cttgttaagc aatttatagg
     4561 tggaagtgga agtggagcgc ctgcaaatcc ttctgaattt tggaaagata tgaaagttga
     4621 aatggaaaaa tatcaaggtt ggccgtatgt ttggggtggg aaaaatccta caacagggtt
     4681 tgattgtagt ggcttaacag cttatttata tcaagtttgt gccggaatta cgattgatag
     4741 ttacacagta agccagtata acgcaagtga acgagtatca attaaagacg caaaagcagg
     4801 ggatcttatt ttctttcgtg gcacgtatgg agcgccagat ttcatttcac acgttggtat
     4861 ttatgtcaat gaaacaacta tgtatgacag taatagtagt ggaattgggt atcataattg
     4921 gcaggattcg tattggcaac aacatagtcc tgaaatacgt agagtaaaaa aataagcaaa
     4981 ggggaaaaca atatgatcaa ttatacaagt acaacaaaat ggaaaatcat gactttaatt
     5041 ctgatcagtg tagtaatatt tggtggacta ttcgctatgc taaataataa aaataaacat
     5101 gacgagaata agaagctgga aacaaaaatt gcggaattag aaactaaaaa tgagaaacta
     5161 agtagtaata tcaatgattt aaatgcaagt attcaatcag cgacaagttc acctaaaggg
     5221 gaagagttga gtaaagaaat tacagatgtg gcgtcaaaat ttgttgagct ttacccgtat
     5281 tatgatattg aaaaagtaga agataagaaa aataatctat tagaaattgc ttcaaaagaa
     5341 gttgcagatt caatagttcc tgaagatatg atctcgtcta gtaagaaaac tttagaaaca
     5401 aatagtgaag atcaaaaagg cgaagcgtat tcaagtgatc caacatttag aagtcgatat
     5461 gaaagtgcca cgctgtacac aaattttgta aatgcaaaac aagttcaata ttttgcagaa
     5521 gtaaactatg aaacagaatc aagtagtggc gatacaaata acactgtttt tattcaattt
     5581 gaagtgacta atgacaatgg aaaagtgcaa gttagtgatt atgaaatcaa atatttgaag
     5641 taaaaaaggg gaatcaaaat gaagaaaaga tactcaattc atgataataa aaataaaaat
     5701 aaaaaagata aatcacaaat tgacaaagtt tatcggaaaa caaaagggac taaattatat
     5761 ttaggggcaa taattagttt tctatctatt gcccttttaa tcgttttatc gccactagtt
     5821 ttaggcaaat catatgatta tcaaactgta gatttaaaca atcctatgaa tatttcttct
     5881 tcactaaata tgtacattga tgatatggaa atcaataaag aagatggact atttaagtta
     5941 gttttgcggt atcaagatga cactaaaaca aaatcattga gtaatattaa aagtgattac
     6001 aagctaaact atattacaaa taaaggaaac gattcagcaa aaatgaaggt agttaaactt
     6061 tcagatgaat atactgtagt ttactatcag ggactaccga aggactttgg tgtaatttca
     6121 gttacaatta aacctcgtta tgtttatcct gagctagagc cttcagatga tttgaaagat
     6181 aaagaaatta aattttacgc agtcgatcaa gacataaagc aaaataataa gttgatagtt
     6241 gaatcaatgt cagacttaaa aaaagacaat tttacttatc aaacaaaagc aattagagca
     6301 gaaattgaat tagaaagaga taaaatcaat aagctaaaat tagcaaatga agttgtcgaa
     6361 aaggatattg ctaaagcaga acaagagtta gatttccaaa ctttagaaga acaaacagat
     6421 acaaaaaatg aaattaattc aatgaaaacg actatcgaaa ataataaatc acagatagaa
     6481 gaaagtaata aaaaaatcaa tggatatgaa gaaaagatta atcagctaga gaaaactgca
     6541 agtagttttg aataagaaag agttcattgg acatttaatt aattgaaaaa ttaactgata
     6601 aaaataaaaa gagagtgatt ggttaccatt aagttcggta acaatctctc tttttttatt
     6661 ttgcaaataa acaatgaaac agggtctaag ccgttgtagt tcaacaggga gtaaaacagg
     6721 ggttagtagg ggtttatata aagggacaaa aaaggggtta ataacaggga aggaggagca
     6781 ttttagtggg ggcatatttt ttatggctat ttatttagga aaccagttgg caccgccaac
     6841 ttcccaccta tcaaatttga aggtgggatc agtttcccct tatgctcttt cattaattta
     6901 ttaggtgttg gccttcagtg cttcaaaaaa agcaaggtat ctgttataat tattgtatat
     6961 cagatagata ataattagga gatgattata tttggaagtt aagattcgtg gtgtcgatcc
     7021 acaatatatt caggaaattg atcgcaaggt aaaggaaatt aaacgaaaaa caaatcgaaa
     7081 ttttagtcga aacgattact tcaaaatgtt gattaggcaa gatagtgaaa ttgatttaat
     7141 taattataag aaggcagaat tcgatttagc tttggataaa atgcttgtgt caaatgaaga
     7201 attaactgca agtattgtca atttaacaga ggtatatgaa cgattattta acttattact
     7261 tagtgaaggt ggtagataaa aatggctgat ccatatatta aggtgtattg ttcagaagaa
     7321 acaaaaaaat tgtttgatga tttttacgaa aagaataaag ggagtcttgg aacaaaagct
     7381 aatatgttcc gtgtgatggt ttcaaacttg ccactgttag ccagccaatc aaatattaaa
     7441 ttaaatgatc cggaaagtaa cgaacttaaa gaaggaatgt cagaattaca gttgatgata
     7501 gcgaatgaag taatggaaaa attgaataag attgacaaaa tggtagaagc aatgtttatc
     7561 aatggcgaaa taaatgatac tgaagcaaaa ataatagaat caaaagagtc aattaatcaa
     7621 gagaataatt cggaatataa ggaaggaatg ttctttactt caaatagatt ggggcgttaa
     7681 tatgtccgaa atgaattggt ttttattgct tcttggcggt atgtcactgc ttgttttaat
     7741 agcgataatt attggggagt ggggagtaga aaaaagctcg aagtccaatc tacaaattgc
     7801 aaaaaaaatt aagaaaaaat gtcgttatgc aaagatatta attgacattg agcttcctga
     7861 ttataagaaa atgtggggtg gtcgaagtaa agtaaaagtg gatttgattg tcatacatgg
     7921 tgtcaatgtt tatgtacttc attttgtaaa tgtagaaggg tatgtgtacg gacatgaagc
     7981 tacaaaaata tggtggtgtg ttcacaaatc taaaattaaa tttatgaatc caatggaaat
     8041 aagttacatg gaatcagaaa tagtgaaggg aattatttct gaaggaactg aaataatccc
     8101 tattgttgtg tttcctgatg gaacaaattt aaactatata aatgttatag ctaatgacat
     8161 taagataata tatgagtcac aagtagttga atacatttgc tcgaaaaagg caaatgtaag
     8221 taaaagaaac acacaaaaat tatatacaat attgcaaaat gcaaactaaa aaattctaat
     8281 gtcaaaaaca ttaagaacgc cggtagaaaa ttttttgtgg gggagtgaat catggccgaa
     8341 catttcttta accgaaagag tgcaagtata atattgaaag ctaaatttga aacaggaaaa
     8401 agtagcaaaa aaaaccatat ggttgaactt caaaaatttg tagattatat agctcggcaa
     8461 gaagcgatta ggaaagataa tatagatcat gagtattcaa aagatgaatt agatgaatta
     8521 tcacgtatag aaaaagcgct aagtatcatg gaaaaagaaa gtgatcaacc agttattaaa
     8581 gatattatgc aaatggataa atatattgac tatatgactc gaaaaaaagc tatcttggaa
     8641 aataaagata atgaaattgt aaatggagct tttagtaatt caaaaagata tataactcaa
     8701 aaagacgtaa agaaaattaa agagtctgtt attgaagcga agaataatga ttcggtaatg
     8761 tttcaagatg ttatatcgtt tgataatgat tttttggaaa gagaaggtta ctacgatcca
     8821 aaaacaaatc aacttaatga gaacatactg tatgaagcaa ctaaaagtat gatggaaaag
     8881 ttgaaagaaa aggaagggct tgttgatcca ttttggttcg caacaattca taggaataca
     8941 gaacacattc acattcatgt aacttcaatg gaacgtcaaa acacacgaga gatgatggaa
     9001 tatgaaggtg tgttgcaggc aagaggaaaa agaaaacaat ctacattaga cgatatgatt
     9061 tttaagtttg gcaataaaat gatcgatcgt actaacgagt ttgaaaaaat atctaagttg
     9121 agaaaagatg ttccattgga aataaaagaa tctgtaagga atacactttt acaagtgtgc
     9181 atagataata attcaaatta caatactgaa ttagaaaagt atctagcgga attaaaaaag
     9241 gaaattccaa gcaacacaag aggatataat gaactatcaa aagaaacaaa agaaaagatc
     9301 gatcatgtta ctgattattt aactaaagat aatccaaaac gacacgaata cgataatatg
     9361 acaaggacaa tagatcaact ttatcaagaa acttatggaa agcgatatac acctgaaaag
     9421 ttctatgaag atagaagagt agatttaaat gctaggatgg gaaatgctgt tgttagtcaa
     9481 ataaaaaaaa taaaacaatc ggaagagatc ggaataagtg gagaggaact gaaaaaacat
     9541 ttaaacaata atgcgttgcg attacaggta aagcataatg atattgatct aaagtttaaa
     9601 acagaagctg gccggtatcg ttatgaaaaa ttggtagaga aacaaagtaa atcaaaagaa
     9661 gagcaagaaa aacgaataca aaattataaa aaaagacaaa tgaaatttca aaataaaaag
     9721 gatttaaggc aaatcgaaag agcgataaat gacgatcttc atatgtatcg tgcaaaaaaa
     9781 gattttgaac aggctcaaaa aatggtagaa caagaaaaaa taagacaagc atatgaattt
     9841 gattttaact aaatagaatt tggaggtttt tatgagtaat ttgaaaggta aagtagaaaa
     9901 ataatttgat cattttgcat aataagtatt ctaggtaatt gactaaattc aatatgtacg
     9961 ttataatgta cataaagaga ggtgtttact ttggaaacta ttactccaac aaatttaaga
    10021 aaagatatgt ttaaggtgtt gaaggaagtt gcaaactcaa actctgaggt agaaattact
    10081 atgaattcca aaaatggtct aaacgatggc attatcatgg tttctaaaag agaatgggat
    10141 agtttgcagg aagaactcta tttacaaaga actggtacat tagatcatgt atttaatctg
    10201 atggaaaatg aaaatgatga tgactttgag gtagtttaat atggtatctg attggcaggt
    10261 gaaagtgcat aaggatgttt taaagaagca aattccttta ctgagatcag tcgggttaat
    10321 atctgattat gaagatatta ttgatgtctt aaaaaaaaat ccgtatgaaa acattaggag
    10381 tagagagaaa ttgaatcctc gtaataaaca aattttttct atgagaatca acagcaaaca
    10441 tagagttgtt tatactatag aaaaagaaaa gcatttagtg aaggtttgga gtgcatggtc
    10501 acattatgaa aaagggatac ctaaatgaca actaagaaaa gggaactaaa ttgttccctt
    10561 ttcttagaaa tctaatgact acaagtagac aaaaggagat tgattatgaa tgggaaaaaa
    10621 tatgcaaaaa ttgtaaaaaa agtaaaaggg ctactagcga tcgccaaaga tgaaaataag
    10681 gatgaagaga gccaatcagc gtttctaatg gctcaaagat tgatgattca atatgacata
    10741 gacaaagcag acgtgctaga agaaggggag gaagcagagc ctattggtga agagtcagtg
    10801 acggtttata aaaaactttt ttggtgggaa cggactcttg gtcaaattat tgcaaaaaat
    10861 tttagagtga agatgtttta taattctaaa agaaaaaatg gtgatcaacg caaaaagtcg
    10921 aaagtggtat tttatgggtt tggaaaagat ttagaattgg ctaaagaaat gtatatatta
    10981 gcttacgagg tgttgcttta tcactcgcaa aagtatatca atcgttggta tgaagatgaa
    11041 gggattgcga gagaaaggta tttaactgaa agcattaagt caagctatat tagagggttt
    11101 ctaggaggat tagaagctcg atttgaagaa caagttgcta tacttacaaa aagatatgag
    11161 gttatggttt taattcctaa agaggtggaa gaatcatata aagactattc taaagaattt
    11221 ggagtttaca attctaaaat cccgcctgta actgtaggaa atgcgtattc tagaggatat
    11281 gaagaagcaa aaaaagtaga cttcacacaa aaaacagtgg gtactgacta ctcaagtctt
    11341 gtggatcgct atattcgttt taatgaggga ggaactagag gactgattgc tgaagtaatc
    11401 agtatttcag aaaatcaatt gaacttattg attatgaata ctgtttcttc tggtttttct
    11461 acaagtaatc cttgttttta ttcgtgggtt attcatgcag aacatgattt tgaactcttg
    11521 catttgacag atgattccgt gcgacttttt atagattaca aaaacaataa taactacaag
    11581 aaacaaatag ctgaattgat aactgaattg aggtagcgga gtatctgcta tctcttttta
    11641 ttctaaaata actctttaaa tcattgcgcc caaaaggtac tataagtaat gaagagtata
    11701 ttcatttatc agaatatgat attataaata tatatcggat ggataaaaag aggtgtaagt
    11761 gatgaaattg aagagtctag gatttttagg attatttgtc ggatgtgtaa ttggtttaac
    11821 tgcttgcagt caagcagaga gtagtaagaa gtctagcgat aaagaaattg cttttgctaa
    11881 ggtagcaaaa acagatgaac agaacttatg gtttttgatt gaaggtgatc aaacagctaa
    11941 gacaccaata gaacaggacg caaaaatcga ctctgtcatt gttactaaaa acggaaaata
    12001 tgaaatgttt aaaacggaag taggaaatct atcattgagt gatgtttcta ctatgagtga
    12061 tgatgaaatc attgaaaatg cggaaaagta tgatcaggaa aaatttgaaa ataaacttca
    12121 aagtgataca aacgattatg agtctatttt acagtcggcg aaagaggaat taacaacaga
    12181 acaaggaaga gaattttctg acaaaagtaa aatcaaagaa ttagaagaga aaatcccgct
    12241 gttagaaagt gatatggcaa aattgaaatc tatagaatat caagctccta aatggaaaga
    12301 aatttctgtt gaaattaaca aaggaaatga cggagaaaaa gctaaaaatg aaattattag
    12361 tctctcgtac aattatctaa gtagcccgat ttggagtttt tcaaatttta aagagttatc
    12421 cagtgaagga gaaaaagttg agctgattga taatagcaag aaattagaat tggcggttcc
    12481 tgtagcgttt gctgaagtga ataaaaaaat atttactggc tatacaaata aaaatatgga
    12541 aacaatactt gttaagcaag ttcataataa ggatcaaaaa atggttctag acgaagaatg
    12601 atatatgcag taaagaggat aaaatcaatg attatcctct ttttttagtg cttaatttca
    12661 atagatttaa tagcgatatt aatgtctatc tgatataata aaactatggt ggataacacc
    12721 aaaataaaga aaggagaagt tcaaatggct tataggaaag gggtctggca gccaaaatcg
    12781 aaggaggaaa aacaaaagga attagatgaa ctaattgaac tgtctaataa gaaaattgaa
    12841 gagtatagag ctgatccaaa agatatgatc gagtttgcta aattcatgtc aaaaatccat
    12901 aactatagtc ctttaaattt atcgttaatt gacgaacaat ttgaaggagc gatcgctgta
    12961 ggaagttata aaatgtggag tgaacaggga tttagtgtta ggaaaggaga aaaagggata
    13021 ggcatttaca ctcatgcgcc tgtgacaata ttcattgatg aagaaggaaa agaaaaaacg
    13081 ctccgagaag ctacggcaga agaaaagaag aaaatcaaaa gtggagaatt atcaagccgt
    13141 cgtatcgaac atttcaagaa ggggtatgta tttgatgtat ctcaaactac tgctactgaa
    13201 gaggatttgc ccaaaatatt ccccaataga gtttggaatt ttaaggttga tgaagaaacc
    13261 aatttagatc aacttgaaag gggtgtagat actcttgcta atgagttagg gatttctatt
    13321 aaagatatgc gggaatctat ggtaggagag ttgggaagtg caaggggagc gtattttcag
    13381 tatgtggacg gaacggaaga gatagctttg aatttcagaa atagccgtac tcagaatatt
    13441 gctacaagta ttcatgagct agctcacaag aaaatgcaca atcgattaga aaaaggtgct
    13501 agttatccta ctgaaataaa agaatttcag gcagaactga cctcgttcgt tgtatgtaat
    13561 aactatggta ttgatacttc tgattttact ataccgtatg ttgcaaattg gacaaaaaac
    13621 aatgagaaaa tcaattctaa agaatttcgt agtattttgg gggatataaa gtctacagcg
    13681 cttgaattta ttgaaagtct ggatagatca ataattgaac aaagagagat aatgagtgtt
    13741 gagaaagatt atgaaaagaa aacttcaaat gaagcacaaa gtgcgctaaa aaattccttt
    13801 gccaatgatg gtaaagaggt agaaaaaaag aataaaatat ttgaaaatca agtgttcgat
    13861 cgagacagtt atatattttt taaaaatgga aattttgacg gttatcggtt tgtagattgt
    13921 acgtttgata aagttaatat gagtaacagt agctttaaaa atactgtatt tgaaaaatgt
    13981 aattttgaat ttgctaatat ggaaaataat gattttactg aaatgtccat gtatgattca
    14041 acaattcatg gtggaagtat gagaaattca aaccttaaaa attcgactat ttggggaacg
    14101 aagattgatt attttaatat gagtaatgtc aacttgttat cgactacttt tgaggatgta
    14161 gatttttcaa aaattaagat aggtcaatca attcaaaatc ttgattgtgc taatttctat
    14221 ctaagtgggg ctacagctga agaaagtaag gtctaccaac aaattaattt agagaaatta
    14281 aatcgaatta attttaatag tgagttcaat cctgatacta ttaagttgcc gtttcgggaa
    14341 tacttttctg atcctgaatg tcctgaaaga gaaatattgc gttcgtattt tgaaggcatg
    14401 attgaatttt ataaggaaaa aaatccccaa accgaaatca aggtggtata tcgagaatat
    14461 gatgttactg ctaagattgg taaaaactat gcaacgtata tgtcggatgt aaaacagttt
    14521 gatatttgtg atatgtatgg agtcacagca gaaaatacta gtatcgatga aaatgatcac
    14581 ttgatattag atagaacacc ggaaaattgc gattatgtag aagcgtttat tcatgaaaca
    14641 attagagctg aatcattaga aaaacaacag attcaaaaaa gtgaagtagt tgctactgaa
    14701 ggagacgtgt atcattttca aaaaagtgaa ttgccaactc cattaaaagt gattgctact
    14761 gaagaagccg tgtataattt atctgattca gctaaacagt attatgattt aaaagagtgg
    14821 tctgaagagt ttacagccca atctgaaata gataatgtgg aacaattgct ttcttgtaac
    14881 gaagcggaag aattttatga aaagcataaa caagaaatag agaataaagt agatgatttt
    14941 gaaaaagcaa caggaatcga ttttagaagt cagttatctg aaagtaatta tcaatcacag
    15001 gttgctactc ttgcgtataa aattgttgct gtagaattaa aaaatgagat agaagaaagc
    15061 caatttgaaa tccctttaaa ttcaaaacta aaaaaagaag agctttcttt gtcatatgaa
    15121 atggagtaaa aaagatggaa aaaaataata attatttgag agcaggaaag tatcagttat
    15181 ttcatggaag catgagcaaa tttgatcatt ttagtcttga atacaagggg cttaatggta
    15241 cagctgatgg gtttggcatt tacctaacgc ctaataaaga tatggcgagt atgtatgctg
    15301 agcaaactaa aaaattaggg tatatatacg aggttacagc tgatttagaa aaaccgttat
    15361 ctactgatga attaacaatt acagataaag aattatcaaa aattattgat attctacaag
    15421 agtctaacaa tattttgaat gattttaatg atgttgatta ttatggtgaa aaggtagtta
    15481 aacgagaagc tattgcaatg ctaaaggaaa atgacaatga tgttgatcta attaacgaga
    15541 ttgcgaatac cacaggggac actgaaaaga cggcagaagc actttacaat gtcgggcatt
    15601 atacgcatat agttgccaat gaaagattgt tacaagaaga tacggtagta gttgtgttgc
    15661 aaccggaaag aataaacata gaaagagttc aagacttaaa taaagaatta gatgtacctg
    15721 caattactga gaattcccat tctggagata aagaaaaatt atctaatcag gaggaagctg
    15781 ttagattatt ttggaaaggt gtattagaac aaaatagatc atttgatttt gttggagtgg
    15841 attccaacgg aactacgcta cattctggta cgttcgttcc tgatccacag gatagagctg
    15901 atagctggct gatgtatttt gataatcaag gtgagatgat taaacaatat ataatgtcta
    15961 tcgatgagat tgaagaattt aaagcaggga aaaaagatga gttcatgtct caattcccac
    16021 acggagcgcc aaatattgat tataaaattc tctttcatga ggaaaacgaa ttttattggg
    16081 attgggatga agatattaaa ggaattaagg ttcctgaagt acctataaaa gatgtaatta
    16141 taaaggattt tgagcaggag ttagaaacag accggtacta cgataagcag gcgtggcaag
    16201 aacgaataga atatcttaat tcaaatgatc ctagtgctat tctacgcttc tctttagatg
    16261 aagatgttga agagttttac aagagtcact cggatgaaat agaagataaa ttatcttcta
    16321 tggccgaggt gttaggtgta gaaagaaatg cattaattaa tgaatctgaa gccacttcag
    16381 aagtggacta taaaaggtac gcttctatta tggcttataa aatcggaatg gatgaaatta
    16441 acaatgatat tgaaaacgga acgtttaaaa tccctgctat gtcagaagaa acagaaaata
    16501 gaattctaac tgtaagaaat atggaatcta aacaaacgtt tgagatggaa tagtaggtga
    16561 gaagatgttt aaacaaattg actttgagaa agtaattagg gctaatctca tacctaattc
    16621 caattgccat gtttatattt gcgaagatac tcaaacagcc ttccagttag ctctaaagta
    16681 tgggaaatat gtaaggactc atataataag agaaaaaaga gacaagagag aaggtttttg
    16741 gaaagtgtac ttgtcagatg gaggtagatg tattttctgt tcagaggatt atttcttaca
    16801 atctccaata gcttgctcat atttaggatt tgccggttag gtgagattgg atgaaaataa
    16861 gtgaattaaa gaaaattaaa atagtggatt tttgcgctga aaatggtatt gaattaacac
    16921 aagattctag ggaatactac cgcttggctg aacatgactc ttgtgtaata agtgatcgaa
    16981 agaatctttt taagtggaat tcccgaaata ttggtggaga tatatttaac ttcatacagg
    17041 aatattatga ctgttctttc aaagaagcga aggaaaaact tcttaatagc gactatgata
    17101 ttcgtgaaaa tgataatcaa tatataaagg aacctttcac ctataatgta tcaaatgaac
    17161 aaacagtttt taaatcaaga cgatatttaa ttaatgagcg tagaattagt aaggaattaa
    17221 ttgatgattt aatcacgcag ggattaatca gagaggataa acgatcaaat gtccttttta
    17281 tatggaaaaa aaatgaggag atagtaggat gtaccgaaca aggaactaca gccaagttta
    17341 atcaaaaaaa acaaaaaaat ataacatgga aaaaaataca agaaaatagc gaagtaaatt
    17401 atgggtttaa attgacgtat ggaaagccta aatcaatcta tttttttgaa agtgaaattg
    17461 atatgttatc gtatataact caaaaacctg ataaagccca aaatgcaaca tttattagta
    17521 tgaatggact aaagaaaatg actattctac aagctgtcac agatcatttt aacgagttta
    17581 aaagactccc tgaatcagtt gtaacttgtg tagataatga tgaagcaggt cgaacatttt
    17641 ttgatacaga gtttaaagga aaggggatag gaagaactgg cataggagtt actgaagtga
    17701 aagcagattt tcctaaaaga aatggttttg attggaatga taatttgact aagggaaaag
    17761 agattattca aaagatgaat gatcatgaca tttcaaaatt aaaagtattt tgtgagataa
    17821 aattcagtaa gacagttcag aataattatg aagtgagtaa tctataggaa aagtaggtgg
    17881 ataatttcat ggctgaaaca atagcaatac tatctgattc acttcttgat ttagataaaa
    17941 aatcaactga ttacatgata attgaggaac aattaaaaaa tggtaatcca gaatttaacg
    18001 aaacggagaa agcattatat attgaattat ctaaagaaaa agcgtttaaa ctgatttcta
    18061 ttctatttga gataattgaa agatatggat taacttatta tcaagtttgg aaagacaaaa
    18121 atgaaaaggg agagtacact aaaggaataa gtgtggaagc gatcagagat aaatacagta
    18181 taagtatcag ggcagtgaag ccattgctag gttctattgc tgattgggga ccaattttaa
    18241 acgtctagct aatatgctag gcgttttttt gttgatgatt tagaaactga gtaataggaa
    18301 aaattggcga atacctattg actacaagta gacaataaag gtatactcca agtgtagata
    18361 aaagactaca agtagtcagt gaacggaggg tataatatga gtaataaaat aagaattgga
    18421 ataatgatca gaggaattga agttagatca aaagaagaag tagaaggagt ggtattgaac
    18481 gtttatgatc atattgtaat tgtttttcct aaacaaggta gtccagctgt tgtgaggaaa
    18541 aaggatatat cagctatttt taattaggga acagcataaa tttcattatg ttgactacaa
    18601 gtagacaaaa agttataata gctatataaa gtccgagaaa agaaaaataa ttggtctata
    18661 aaggatgata tttagaaaaa gcgataagaa tggagtgaat catatgtctg aagaaactag
    18721 agagttaaag gagatatacg gaaaaattaa acgaatgagt atagatgata ttcatgaagc
    18781 tctaaaaact gcagaaactg aagaagaaag agaactctat ctaaatatga caagctttat
    18841 aatgcaaatg gagcaaaaaa agatcttaaa gcgaaaagag aaagttcatg gatgataact
    18901 tacattatgt tgtagttggt ggtgtgaatg gagctgggaa atcaactatt tatgaatttg
    18961 atactatccg gattgagggg cttaaaacgg atagaattaa tgcagatgaa atattacaaa
    19021 gaaatggtgg aaactggaag aatacaacag acgttgctaa gagtatgcga caagtagtta
    19081 aggatataaa acaacatttt gaagatagaa agtcgtttca ccacgaaact acttttgcag
    19141 gtaatacaag accccatgaa aagcgattat ttatggccaa gtctttaggt tataatacgt
    19201 cactaatata tgtaggactt gaatcgcctg agcttgcgat cgaccgtgta aaaagaagag
    19261 tacaagaggg cggtcacggc gttgatgatg gtatagtgat ggatagatat tataagtcgc
    19321 ttgagaatct ttcaaaactt gaaaaatatt ttgataatgt aacactattt gataattcac
    19381 gtttatttca agaaatatac gtgaaaaaag atcatgttgt tgttgctaat agaagtcaaa
    19441 tttataattg ggtacctgaa actattaaag agaaaaacgt tgaaaaatct attgaacaag
    19501 aaattattac tagttataca aacaaaaata aaggatatag tgaacttgaa aaagactact
    19561 cttcggaatt aggaacatta aaaatatttc aagataaaga gtatcttgat ggtttctata
    19621 accggcataa gaacgaaata agtgaacgag tattgggttt aagcatatct attggaacta
    19681 gttcagaaaa tttgcttggt gacaatccta aacagaaagc aactgaactt gcctataaaa
    19741 tgtgcttagg gaatttcttg gaaaaatttg aagaaaaatt acttaaaact aaaaatgtag
    19801 atcgtgtgag tgagtgggat agagagattg agtaggcgta tgttatgcct actcttcaaa
    19861 aaaaacattg actacaagta gacaaaggta tataataaaa gagtggaagg gttacaagta
    19921 gtcaatgaat gtagctctct atcaaaccaa aggatttcac gttgttaaca tgaaagtata
    19981 ataagggaag atatttctac aggggagatt actatgaaaa actatgcttc acgtaagttt
    20041 atacacttaa aacctattaa agagttgtct atgaatggaa atgagtataa tgattacaaa
    20101 tcattatttg taaaagatac tcctataggt gggattcgga tgaagaatat gccggatttt
    20161 cgattaattg aagtaaataa tctaggaatt gtagaatatt atttagaaag attagacgaa
    20221 gcagtaaact tgtcaatcca acaaaatggt gaagaacata tacttacagt tgttctaaaa
    20281 gatttaaaaa acagattgga atcggaagcg gaatttttct acgaggatta atgtaaaagg
    20341 aattaaattc tattgttatt gcaattatct aactattttt atagttagat aattttataa
    20401 atcaaatgta acgtaagggt ataccctatt gtatcatatg gtaaagtaca gtataataag
    20461 gtttggaggt gtaacattag ggtgtaacac aatgtgaaat ttggaggtca ttttttgaac
    20521 aagaaacagg atcaagaata ttatatagaa aaattggaga aagagaactt agaactcaaa
    20581 gagcgaataa gatactatga atccaaattc cataagagaa gtgattgtat gaaaccaaat
    20641 cttattgaaa caggtaaaag aattaaatca attcgttcta atttgggaat gacaatggag
    20701 caattcgcta ttttaactga ttcttctaat acgagtgctg ttaataattg ggaacgtggt
    20761 tacaacttac ccaataaaac caaactaaaa aaaatagcta tactaggaaa tacaactact
    20821 gactggatta aatggggaac tttagaagaa tatataactt cataccttat cggtatagga
    20881 tatgagctat atattaaaga ctttccagag atacctcata aagtctttaa agacattcaa
    20941 gaaaaatata gtgatacgtt ttcgttagat aaagactatg aattattgga ttctataata
    21001 aaaaatatat ttacaaaata ttactcaaaa gaatttgaag taaaccaaat tccataagag
    21061 aggtgattgt atgaaaccaa atcctgttga aacaggtaaa agaattaaat caattcgttt
    21121 taattttgga atgactatgg aacaatttgc agatttaatt ggaaatgctc aaaaaggaaa
    21181 tattaacaat tgggaacgag gattctccct tcccaacaaa gaaagattaa ttaaaattgc
    21241 tgaattaagt aacaaaacta tcgactggat taaatgggga actttggaag aatacatagc
    21301 ttcatacctt aaagattcag ggtatgaact atatattaaa gacttcccag agatacctca
    21361 caaagtcttt aatgacattc aagaaagata tagtaatacg ttttcgctaa ataaagacta
    21421 tgagttattg aatcctataa taaaaaatat atttacaaaa tattactcaa aagattttga
    21481 agattatcga gacatagaag tcaaaccaga tttaggactg actgatgaca acaaaaatgt
    21541 taaaatgatt ggacaacgta taaggaaaat aagaaaaaaa gagaatatta cattagaaga
    21601 attcggcaaa ctattctctc ctacagctga taaagctgtt ataagcaact gggaaaatgg
    21661 taaaaacctt cctaactcag aaagaattaa aaagattgca gaaattggcg gagtttctga
    21721 actttatctt atgacgggtg tcgattcaag cgtagcagaa tcaatgtatt ttctatctga
    21781 agtagcattg gatgctttgg aacgtctgtc aattgaggaa gtcaatagaa taattgaaag
    21841 cttcgccggt tatcttaatg tgatttcaaa aattgatgat ccagataagc gagaaatatc
    21901 tctcaatgct atttgtcaac tatcacttct cgataaaatg ggttgattca caaaaaatat
    21961 tgaaagatta ctttactcac gaggaggctt agctatgaaa cctaatctat cgttaactag
    22021 tgaacgtatt agaaaaatta tcgaatccaa aggtctgaca ttagaacgat tcggtgattt
    22081 aattggcgac acaccaataa gttctgttaa taattggctt agaggcgtgt ctttacccaa
    22141 taaaaatagg cttttaacaa ttgcaaatat tggaaacact agtgtagact ggattaaatg
    22201 gggaactttg gaagaataca tagcttcata ccttatagat tcaggttatg agctatatat
    22261 taaagacttt ccagagatac cttacaaagt ctttaaagac attcaagaaa aatatagtga
    22321 tacgttttca ctagataaag actatgagtt attgaatcct ataataaaat ttactatagg
    22381 gggcgttact ttgaatagaa taaaatttta tattgattcc agtaaaaaga ccattaaaca
    22441 aattagtaca gaaactggta ttaactatac aactcttacg aactattacc gtggtgagcg
    22501 aaatcctaga aatactgaaa catggaatat ccttgctgat tatttccatg ttcctgtaag
    22561 ttatttgatg ggattagacg aaacaaatgt tgaactcaaa aaccttaata ttggaaacaa
    22621 catcaaaaat attagaaaaa gtaaaaatat gacacaaact gattttgctg agttaatggg
    22681 tctatcacga tcatatatag gggatttaga aaataacagg agtaacccca gtataaaaac
    22741 actggaagct attgcaagag cattggacgt tccgatagta gcattaatca acgattcttc
    22801 tactgataat atagtagagc cgtatgaaag tttgacagcc gaaaatatcc ttcttaaaca
    22861 agaaaatgag gaattaaaat atgaggtaga aagcttacga aaaagattgt atgaaatcca
    22921 acaagtagta aatcaaggag ctgaaaaaat gattcccaat ctaaaagacg taagtaaaag
    22981 aatcaaatat atcagaaaaa aattaggtat gactatggag caatttggtt ctcatttaag
    23041 caactcacct aaaagtacaa ttgctacttg ggaaagtggt agaaatatac cttctcagaa
    23101 aaagttacag ctgattgcta tagtaggaga aaccaccact gactggatta aatggggaac
    23161 tttagaagag tatataactt cataccttat cggtatagga tatgagctat atattaaaga
    23221 ctttccagag atacctcaca aagtctttaa agacattcaa gaaaaatata gtgatacgtt
    23281 ttcgttagat aaagactatg aattattgga ttctataata aaaaatatat ttacaaaata
    23341 ttactcaaaa gaatttgaag taacccaaat tccataagag aggtgattgt atgaagccaa
    23401 atccttcaga agcaggaaaa cgaatcaaac aattacgcct ttcatgtggt tttactatgg
    23461 aagaacttgg aaggaaaata gataattcac cacgtgcgac catatccaac tgggaacgtg
    23521 ggaccaattt accaaatcca caaaagctga aattattatc aactatcact aattcaacaa
    23581 ttgactggat taaatgggga actttagaag aatatataac ttcatacctt atcgatatag
    23641 ggtatgagct atatattaag gactttccag aaatacctca caaagtcttt aaagacattc
    23701 aagaaagata tagtaatacg ttttcgctaa ataaagacta tgagttattg aatcctataa
    23761 taaaaaatat atttacaaaa tattactcaa aagattttga agattatcga gacatagaag
    23821 ttaaaccaga ccctaaaaag gtagggagaa aaattcgttc tattagaaaa aaattaggac
    23881 ttactatgca ggaatttggg tacgaggttt ctaattctcc tagaagcact gtgtcaacat
    23941 gggaacatgg tggtaactta ccgaacaaag ccaaactaaa aaaaattgca gacattgcga
    24001 attgtagtgt agaagatttg ctttttgatg aaggctttat ttctgaaaaa tcccaagtta
    24061 aattgataaa tgaactcaaa gaagaaaatt caaagctaaa agaaaaaata aaatactatg
    24121 aatgtttatt tgatggtata gagagtctgc tgaactcacg aaaaaattcc tagttaatta
    24181 aactagagta aaattatttg taggtgttaa atatgttagt tggctatgca cgagtcagtt
    24241 cagatgatca aaatttggaa agacagcttg aagaatttag aaaaattggt gtcgaaaaag
    24301 tatttgctga aaaaaaatct ggtcgtaaca cagtagaacg gccagagttc aataaaatgt
    24361 tagcgtttgt tagagaagga gatacacttg tttttgagtc tcttgaacgg ttaggaagaa
    24421 attctgatga aatactcgat acagtatctt ttatagataa aaacggcatg ggactaatgg
    24481 tcttaaattt gccaatctta gatacaaaat tcggtgatcc taatttagaa aaacttgtca
    24541 gaagcttagt gatcagtatt ttttcttgga cggctcaaaa tgagagggaa gaaattaaga
    24601 gaaagcaaaa gcaaggaata gcaatcgcaa agaaaaaagg aatctataaa ggaaaacctt
    24661 acgaatattc ttctacagcg aagaatccgc aaaagcgatt tatctatagt gaaatagtga
    24721 gaatgcttgg ggaaggcgtt ccaattaagc aaatcgcaga aaaaactggt acgaatagaa
    24781 cactagttta ccgtattaaa aaccggattt taaaggagtg aatggtttga atgaaatgtt
    24841 gtttatttta gtggccaata gttttgtttt attaattata ttgacatgtg attttatact
    24901 tataaatatt ggagtacatt cctttattgt taatttattg ttgtttgtat tttctttaat
    24961 tcaagtgatt agcttttgga ttttgtggaa ggaaaatctg ttaaatacta tatatgtact
    25021 gattgcgatt atgcctttac tttacctttt agctggaatt attggtattg ccagaaatag
    25081 agataatttt ttcttataga ggttagttag tggaagaaat acacaactag gaggaagtaa
    25141 acgatgtcga tcgagaatgc agaaattaca gggacgcttc tagggtacgg tgaacacgat
    25201 atattgacct tcagcattgg tttaaaaggc aatgggtggg gagttaatta cggaagtcga
    25261 gctttagatg gatacattaa aaaagaggac gaacgcaaac caaaccaagc agcgatgagt
    25321 gtcattccag aaattttgaa ggtagtcgga gttagaaagt gggaagactt aaaaggtaaa
    25381 tatgtacgag ttgaaacgaa tggactaggt agaaccgtca ctaagatggg gaatttaatt
    25441 gatgatgttt ggctggattt agatgatttt tttaatactt gattccacta tccaccaaaa
    25501 taagcaacta tatgggagtt atcttttgct tttttgaatt agcagaaatt tttatgctga
    25561 gaggacgatt ctaaggctac ttcagttaaa taaggaaggt aattaagcta ctgcataaat
    25621 agtggtttaa tttaaaagga gtattgtatg tggggggtaa tttatctagt tctatatgtt
    25681 ataggaaaac taacagattt aatttatttt aatcaagcaa tatgttttat aatatttatt
    25741 ttagtaataa ttattataaa tgcttgtatt tttaggtcag gacgaaacaa aaaaagaaga
    25801 atagataaaa cagtcctatt aaatttaata gtgattatca ttggagttgg aacctttaca
    25861 agcaaagctt taatttcaag tgttatccta ctaatatttg attttttgat tatctatagt
    25921 gtgttatatt ttattaataa ggctcgtgaa tatttaaacc aatagaaaac ggtctaataa
    25981 aatattggag gtgtttaaaa aagctacaag agataggagg atttaaattc atagagatga
    26041 aggttcaaag gagagtaaaa aatgacagct atttttaaag cacgaaaaaa ttctaacgta
    26101 tctgaatacg ttaaggctag gttgcaaaaa aataatttag agtatattct ggaaaacggt
    26161 gttggaaatc ttctaaatga actgactgaa gatatagata cagattatat tgttttgtgg
    26221 gataatggaa cacttgaagt atctatttat ggagactttt atcaaactca ctcaaaggca
    26281 atgtttaaaa aaagtttgag tggaagaagt gacataaaag gaatcagaaa aaacagcagg
    26341 ttttataagg aaaatcaaga agtgtttagt aagcttgcgg aaatcatacc tagtatggta
    26401 gcgcccataa gcaaacgact gagtaaaaaa tacttcggaa cagtttatga tggattcggc
    26461 ttgaaaagca acgtagaaaa gtattattac tttgatgata ctgcatattt agcaatcaaa
    26521 gatatgaaac gaatacctac agaaacactt gatcaattag agcagatgaa actttctgaa
    26581 ttctattcaa tctatgagga agtggtaaaa gtgggtgcta ctaccaatgg ataatcaaaa
    26641 agtcggaaat aagaagagta agaaaaatga tttaaaaaaa gagttgataa attttagaca
    26701 aagtctgaag gaacgagaat ataacttaat atttcgtctt gatgtttgga gggcgctaaa
    26761 agtaggttct ggctttttag gctacatgat gtggttgtat ttagctagaa ttggatggat
    26821 aaatttgtat agtattttcc gtgtatttgt agaggatgaa tcagtaaaag ttggaatcaa
    26881 cacgtttggt aatttgatgt tcaatctatt attgatagcc ttaattgtgc tacagtcagt
    26941 taaaattcct ttgacaaaag aattatcagg gtatacttca atcttaaaac cctttcaaga
    27001 ggggttaaaa ataatttgta agcaaggtac tgtggttttt cttattggaa tatttacaga
    27061 tgaaattaac tttatcgact ataaaaacgt aataataagg ctgtgttcat ttttattggt
    27121 gtatttaata gcaacaactt ttgaaaaagt aactttcagg aaaatcgaaa ctctggtaca
    27181 aaaagaaatt tcaaaagaag aattttgtct ggaaactcct tacggaaagc tgcaaaatgt
    27241 atcacttagt acattttgtt tgaatagtaa gtgggaaaaa tatctatata gaagcgaacg
    27301 aagggtgtca tggggcagtt atagtccaat tctacatgaa tgtctgagag aaactggtgc
    27361 ggtagccgat ggtgaaatta tggtgctgaa ggatgtcttt ggtgactatg aaattgcttt
    27421 tcttgatcaa cctgaattaa ggagtgattg ttctgcaaat ggttaaatta ctatctaaac
    27481 tttcttatcg aaaaagattg tcaaatacgt cggaaaatga aaagacaaga atagtagatt
    27541 ctaggccttt tagagtatgg ataaaaaaag agaaaagaaa gcgctaggtg cgttttcttt
    27601 ttttgctaaa gactatttgt agtcaatgaa acaacaaaaa aagcgactac ttgtagtcac
    27661 ttccaagaaa agtaagagcc agtgaaagct accaacaatc aactggctca caagacttat
    27721 cgtagcaaag agaatttttg gcaagcatac gaagtcttat agttcaattc tgatatttta
    27781 attataaggg attaacgtag ataatgcaag gggaaatgac ttattaatac ttagaaacaa
    27841 atataagttg acaatgatta ataaaaattt aaggtaatcc gctatatgac tacttgtagt
    27901 cttatagtta gttttatcct tgactacaag tagtcaaaag cggtataata atagtgcaaa
    27961 ggagggataa tgtggagtta accaaaggga aaagaatagc agttgttcga tttgattctt
    28021 tgtcgataaa tgattcaaga tatattatgc ctgttacaga tgttgtgttt aaaacggaag
    28081 atgggataag attgatatgg cgtttgccga tcaaaaagag tgaacgatta ttaaaaaaaa
    28141 ttaaggtact tgagatagag tttgttgtaa ccgaaaagat tgcgccagaa acctatctta
    28201 tagagcaggt aaaaatattt gggtgataaa aacaccaatg aaaaaaactc ttgttaaatc
    28261 ggctggaaat agcttataat aaacttagtt ttgaagccaa acaaaacact aaaaaattct
    28321 agtaatcaat tttgcaggtt gattactaag tatctaactg caataaagga atattagcaa
    28381 aaaagcacat gaatagagct accaacttta ttcatgggaa ctcgtagcaa agagaatttg
    28441 gcaagcatac gaagtctaag aaatgagcta ccaactcatt tcagtattgc gctaccgtca
    28501 caacaatgaa agagaaagta gatacatttt tatctatttg tagttttcta aacattgaat
    28561 tgaaggcata gtgtatattt taggactcac agctacgaga acctagaaat atattctatg
    28621 tcttttttgc tatctaaaat tgatagcaag gagaaaatca atgttggatc gacaagcata
    28681 catggatgtc ttagaaggca ttttaaaaga tggattgcaa aaatttaata gcaaaaatgt
    28741 tatgccttta aataagtctg ttaacaaaaa aggagctatt gtaggctttg aatcaaaagg
    28801 tcgtttgatt caaagccaag gtataatcta tcgatctctt gaaaaacttt acgctgatag
    28861 cgaattattg acacattgga ctcctaatgt tttctcatgg attggtgggg gaaaagggca
    28921 atcagtttgg gggcatagag aggataattt aattcaaata aatacatttg ttgctgatat
    28981 tgatttcctt gacaagaaag acaaaatcag tttgaacagc cttgttcttt atctactgga
    29041 agaaggtctt ttaccatatc ttattttaga tacaccaaaa gggtatcatg cttattttct
    29101 aattaaaaac tataacgaag agtcaagaga gtacgacaag gcaagttata tttcaaatgc
    29161 gaatggttat aaatcgctcc atgttgctaa aagaatatct gaaaatataa gaaaagcaat
    29221 taaaaataga cttccagaag ttgatatggg gtgcaatcat tttggcgtat ttcggtttcc
    29281 tacacctcgt aatattgtgc attttgaacc taatttcaca aatacctttg agggatattt
    29341 agagtggtca aaagagtttg agagaaaaga aaaagaaaag aaaaaaggaa atatgactgt
    29401 actctcaaat aaaactagga ttaagtctaa ctttcgccag atcgatacaa aatggtacga
    29461 ctaccttatt catgcagata ttgaagaagg ggatcggaat agtgttattt ttacactttc
    29521 attggcctgc aaacaaagcg gattatcaat ggagcaatgt attgatcaaa tggatcaaat
    29581 tgagtttaaa aatgatttga gtaataaaga agttcaaaga acaataaaga gtgcatatga
    29641 gggagcgtat tcgggagcta aaggctttta tattaacgaa cttttggaga aatatactac
    29701 acctagtcaa ttcaaaaaat acgctgtcgc tacagagaat aagatggttt ataaaagaaa
    29761 aggcaatcgt gttgatccat gctattgggt taaatttgcc aaaaatagag aagaacgtac
    29821 ctatagccat tttagtgaaa gtcaagatga tttgatggct tatttgaaaa gcaaagaaaa
    29881 gcagttaaat gaaaaccagc tgtatttaga gttgaatatg gtcaatttat ctgaagaaac
    29941 taagattcct ttatctacgt taaaagtaat aataaaagag ctgaaacagg ataaagatat
    30001 aattgtgaaa actaagcgtg gccggaatgg cagtacaaag atcgcaacga gaagatttgt
    30061 cgaagtcaag gttttaatgg ctgtcataag ccgtaacaag ggaaataaac tgtttgctac
    30121 agaattacta gagaaagatg aacaaacgct tgtagaagaa attataagcc gatatgagaa
    30181 taaagcatta ccttttagtg tagataaaga aaaagttcag agagaaaaaa ttgcaccaat
    30241 tagaggagct acaggttaaa acgcctttat tttttagagt tttttaaata gctaaattgc
    30301 cttgtttaca tatctagctt tctttcctgt gcatggcagt ctaagctaga ataaccaaca
    30361 tagtgtctta gctatatgtt ctttgaaaat ggaatacagg agaatgggac tggtctttac
    30421 tagcgtagtt tatgagtaaa tgtggtgata taagcgttga ctacaagtag tcaacgcggt
    30481 ataataatgt taattaatag cttgaggggg attctatgtt tacttcagca gacgagaaag
    30541 ttatcgaaca gaaagagccg gatcgtaatg gaactataaa cggaattaag agaattgttt
    30601 gtaatcgatg taatggttcc ggaagtttta aatatgtgtc tatggggaga ttgacttgtg
    30661 gagtttgttt taaatgtaat ggtttgggat atgtagataa aaagtttaaa attatgacac
    30721 cagagtatag agctaaacgg gacaagataa atttgtgtaa tcaacaaaaa gcggaagaaa
    30781 aaagaattgc gaaggaatta gaaaggcttt attggcagat taagcagaag ggatttgatc
    30841 agcaaattat ttatatcgtt aatgagccgg atacttatgt tattaaggat gatttgaagt
    30901 cagctggtgc attgtatgat gatgtcattg gatggtattt tttaaatgcc gttaaacact
    30961 tttctactga aatggttctg actcaagata tttatgaaat caaaaattct gaattaattt
    31021 ataaacagac ttatttggat aaatctatac aaacagaagg tgaatttctt tttagtgtag
    31081 gtgatagctt tgaattggta gtaacatatt caaagtatga aaggattgac aatccctttg
    31141 cttacaaggc ttacatttat ttttatgaat ttttagatga atcaggtaat ttatttatat
    31201 ggaaaactag taagaatgaa attgacgttg ttcaaggtga aaaaatttgt ataaaagcaa
    31261 ggctgaaaga tcataaagaa tggaagaaaa ggaataaggt tatcagacaa aatatgatca
    31321 agaatgttag aataattaaa gactacaagt agtaaagttg gaggaaacct ttctttatta
    31381 gtggttattt ttttgatata attaatatac gtcagataga taataaaaga ggtggttgaa
    31441 tgaactctga tggtattttt aataatgctg attttagaaa gggggatata acagtaaatc
    31501 gttcagacta tgtatttcct gaagaatcag gtgtatttgt aattgaaggt gtgactgttt
    31561 gtcgctactg gggaaaaaga aaaaatttaa tcttattgtt ggattcgttg gatggaagaa
    31621 aacttaaatt ttcgttttgg agtaatacaa agtattgttg tgagccttcg cgttcaagag
    31681 aagtagatgt tgaaaaagag agtgttaggt atctaaaaga tgggtctagt ataattttaa
    31741 cgtgtgaaaa aagtaaagac gcaaaaatta caaggataag aaatatagaa ataaagaagt
    31801 gactacaagt agacactgtg gttacttgta ggattgtttc taataaattt atagatagaa
    31861 ggttaaacta tatgggaaca gtaattttgg cagaaaaacc ggatcagggg aaaaaatttg
    31921 caactgcatt agcaggaaag actccggtaa ataagggcgg aaaatatgaa tttgaaagtg
    31981 aagtgtttgg acatacaatt gttacttggg ggatagggca tttagtcggt ttatcattgc
    32041 ctgaaaaata tgaatggtta cctaataaag aaaaatggga tttagccaat cttccttttc
    32101 ttccaaaaga gaatgaactg agatacgaag tttccaaagg aaaaagtcaa caatattcta
    32161 cggtaaaaag ttgtttagaa aatgcagaca tgattattat tgcgactgac cctgatcgag
    32221 aaggcgagaa cattgcatat aatattttta aactatgcaa aaaaaagatt tttgaaagac
    32281 caataaagag actttggatt aattcacttg tgaaaacaga aattttaaaa ggattcaaga
    32341 atctaaggga agcagaggaa acagttgatc tttttaaaga agcaaatgct aggcagatag
    32401 cggattattt agttggtatg aactatacag agttattcac tttaaaattg caggaacaag
    32461 gattaagagg agtcgtatca cttgggcgtg ttcaaacacc agttaataca ctagttgttg
    32521 aaaatgatca agcaataaaa aattttaaat cagaaaagta taaagtgatt gagtgccaaa
    32581 caaagaataa ggaaccaaaa actattttta gaaatagtca tgaatatttc aacatggatg
    32641 agcttgaggt agacacaaaa aaattcggct tagattccgc cagtacagga attatcaaat
    32701 caatagaagt tacacaaaaa caaactgaac cacaaaagct ctttagctta ggtggaattc
    32761 aagaatatgc aaatacaaaa tggaaatact cttcaaaaaa gacaaataaa gtaatacaag
    32821 atttatatga tcagggctat ctttcttatc caagaacaga tagtgagttg attactacga
    32881 atgagtttga gtatctgaaa aataacttgc accgttacaa ggaactatta aagttaaata
    32941 taaatacgcc ttgtgtacag ccgaataagc gatttgttaa caatgataag gttttagaac
    33001 actatgcttt aattcctaca atggaaattc cggacatagc aaagttcaaa actgaagaaa
    33061 aaaatattta tgaaattatt gttaaacgaa cttgtttaat gtttgcagaa ccgtatatct
    33121 acgagaatac aaaagttgtc ctagatgtaa atggttatga atttatggca aatggaaatg
    33181 tacctgtttc acttggatgg aaacaagttg accagaatat tgacgaaaaa aaagatggtg
    33241 atggcgaagg tgtgtcatta ccaaagttta tagaaggtga agttgttaat ataaaagtga
    33301 attttattga aaaaaacact aaggagccga aacgactaac tgaagggaaa ctagtaggta
    33361 agacaggaat tatggccaag ttgggattgg gtactcctgc aacacgagca ggtattattg
    33421 agacactttt agcacgtgaa tacattagaa tcgaaaatac aaaggtttat cctacgccta
    33481 aaggctattt attgtgggat ttgacaaaaa ataaggattt gttaattggt aaacctgaaa
    33541 ttacagctca atgggaacag gcattgagta aaatagcaaa aaagcaatat acacgagaag
    33601 aattcttgaa aaatatttat aaatacttag atgttactat tcaagatttg aaaaaatccg
    33661 attttgagag tctgtatctt gaaaaaagta cgatacaaaa taagtatgaa attaacggat
    33721 atgttgttga agagaaggca aaagtatttg aagtagttaa gtctgaaact aaagaaaaat
    33781 ttgtgatatt taagaatatt tcaaataaga ctattacaaa aacaattgtt gaagagcttc
    33841 tcaaaaacgg aaggactaaa aagcctttga caggttttaa atcgaaaaaa aataaaaaat
    33901 tcagtgctat attagttttt gatgatttag agttcaaggt gaaaatggaa tttgaaaaca
    33961 aaaaagctga ttccaataaa ttttaaaaga ttaagcaaaa gagctataac aagttctttt
    34021 gcttttgttg tcttataaaa taaagaaaaa aaggaaatat tggttatgac aaatactaat
    34081 aaagttagtt atactagaag ggagttttag aatttgctat ataaaaacca tttgcttgtc
    34141 agtaatgcga ttgctattcc ttttatgggg atgacgaata cattgacagt tagcagtatt
    34201 gtcgctttaa gtttgggggc tttattgccg gatatagatg aaccggaatc atatgttggt
    34261 aaacgtacac gaggactatc tgatttaata aatatatttt ttggccatag gggaattaca
    34321 catagcttgg cttttgtatt atttataggt ttgctgattg tgccacttac gagtaaaata
    34381 ggtttcgttt ctttaggtgt gtggcttact gtaggagtat ttttgcatat atttgaggat
    34441 agttttagta acagtggtgt aaattggttg ttaccgatta gtgaaaagac ttatcacttg
    34501 aatatatatt ctactggtag tttaattgaa ttcctaatag gcttagtagc tctaatttgg
    34561 atagttgttt gttttaaggg tcagtatttt gtattgagtc aggatgaatt agtaaatgta
    34621 gctgtactaa aaaatatgtt tattgatata aaagatacgt tatcaaaaat cttattggaa
    34681 taaggaaagg aagaaagcaa ttgaaaatta acaactcgcc accgaagaat ccaaaagata
    34741 aggcaaaatt atttactatt gaaaatgagt atattaataa attaaagtca attgacagtc
    34801 gtgttcaaaa taactataaa ggacaacgat tgtattacaa atcagattta gaagtaggag
    34861 agggaataaa ttattatgtt ccactttctt cagcaaaaga aagtcaaaaa aagatcacca
    34921 acaaatcagt atttaaactc tatggtgata aggcgcaaga ggatttttta ggagttttgc
    34981 atattaataa tttgatacca gttccagatc aatacgctaa gatatggtct ccgttagcga
    35041 aaacagatga acgatattta acattagtta ttaagcaagc taggtacatt aaagctcatg
    35101 aagaagaaat caataaagat tgtaagttaa tggtagatag taatatgggt actcttgatc
    35161 ctgtgtattt tagtaaaaat cgcaaagata ttatgaccta taaacggata acgagtgatc
    35221 taacgaagtt agagcaggta agtaaagctg aaagaaatac aagtgattca gtacgtcata
    35281 gaagtaatga catagagatt aattaaaggt aggaaggctt aggcttttct gcctttattt
    35341 atgttggtta agaatgaaat atatgatatt ataattatat atcagataga cataaatgga
    35401 gggtgctaaa tttgtcattc ttagaaaaat ataaagccga agtcctagat gaattagaga
    35461 aagaatataa agaagtgagt gtcgattata ttcaaagtga tctattagat gatagtttaa
    35521 gtgaagtaga attcgtaaag caaacattag agaaaattat tttatctgaa gaaagaccgc
    35581 tcttaacttt aaagcaatta tttgaaaagt atcatgtttt gcagaatact tacgatacag
    35641 caaaatatgt atttcatttg tctacagaaa acttggacga gaatatagta gaaaaagcta
    35701 ttgattattt agctatcgag ttttagagat gtggtaaaat gaacatactg attaaatatt
    35761 ggaaggttga ataaaatggc tagaccaaaa aaatataaaa ccgaagaaga aagaaaaaaa
    35821 gctagagaga aatccaaaaa gaaatgggct agtagaaata aagataaagt tgcttatata
    35881 agagccaaat cttcagcaaa attgtttgtt gaaatgtcgg aagatgaaga tgttgaaatg
    35941 ctagaacgat ggatcgaaga gagaaaaaaa agggggaaga taagtgataa taatgattga
    36001 ctacaagtag tcaaagagat ataataaggc tgtagggagg tattctccct tatttttaaa
    36061 attaattgac tacttgtagt caatgggagg aggggttgaa aaagagaact tatgttcgta
    36121 taatgatgaa aggagagtgg taataaaaaa attaactatc tagtttgtgg tattaatgtt
    36181 cgtttctatt aatagaacag aaactatgaa gaaaataggt tttgaagaat gttgtggagg
    36241 gagttagctt gatagaaaat agggttttag gctttatcga atgtgaagat gatattaatg
    36301 atactttaga agtgttagag agcattgtat ttaatacgaa agtattagat ttgaaagcgg
    36361 tcaataacga tatggttacg catattattg ttgatcacga gaaggctgcc caattgggag
    36421 agattctaat tggatgggcg aatggagagt tgtgaaattc tcaaattttc ataaatgact
    36481 acttgtagac tgctttattg actacaagta gtctgtaagt tataatcaaa ttatcaaagc
    36541 gaggtataga taatgaaaaa acttaatcta ggctatgtaa tggcaattaa tatgttcaaa
    36601 gggggagttg gtaaatctac tcttactcaa ctgatggcag atattctttc tgcgatgggg
    36661 tttaaggttt tagttattga cgttgatcca caaggaacgg taactaaaaa atttcagaga
    36721 caatatgatt tagatgttat tggtagaatg actttgtttg acgctgtaat taacatgtcc
    36781 ttcatggaaa gtatcacaag tatcaaagaa aacttagata tagtgattgg gtctgaacaa
    36841 atgtcacgct ttacaaaggt tttaagagat atgtggagat caactggttt ttcagacgaa
    36901 ggtgaagaaa ttttctggaa tttgtttaac tatatgatca ataatgatga tttgagaagt
    36961 caatatgatt ttgttttatt tgatactatt cctacggttt ctgaatttac agataatgtt
    37021 tatgtagcta gtgatttctt ggtaattcct acgcaaacag agcaagattc aattgataat
    37081 ttgcatagta cggttaacaa ttataggact gctgtaaagg aagtaaattc taatttggaa
    37141 attttaggta ttgttcctta tttagtcgat acaagactta aaacagggaa agaattgtta
    37201 actgagctgc aagaagagta tggtgatatg gtatattcta atgttgttaa cgataaaggt
    37261 gttgtaagac gttggggacg agaaggattg gacaaatcaa ttcctcactc caaaaaaact
    37321 tatcaaatgt atgttaatat tgctgttgaa acattagaac gtcttgaaaa agtaactggc
    37381 gaaaatttat taaattgata gtaaaggtgt tgcaatcatg ggaaaaacga aaaaagttac
    37441 gagtgccggt ggtaacaatt ctggaaatcc gtttggcatt aaaagtggtt cgggtggttt
    37501 taatatcgga cagcaaatgc taaaaggaaa agcaggagtt aaatcagctg gtgacaaaag
    37561 aaaatcggtt cgtgtgccga ttcaactata taatgaagtt gtagcaaggc tttctcatgg
    37621 cgaaaaatat ccgcttatga cagaaatatt aacttcattt ttaaaagagt ttgaacaaaa
    37681 aggagatagt attattcaga attttaatga tttagaaaca cgtccaataa aaatttctga
    37741 agaggcattt cgtatattaa aagtattcaa atttgaaaca gatcagtcta atacagatat
    37801 tattgcttca gctttaacat ggtataaaga gaaaaataaa ggaaaagcat aaaagatcaa
    37861 gaagggggta agtatatgaa tcgtgatcaa tactctcaca aagaagataa atatgtatgg
    37921 gaaaatattt taattttagc agggatcgtt ttagggacaa ttcttttggc ttattttatt
    37981 gtaattccta acttatttac atttataaat tggtttgttc caaatttttg gtcaatattt
    38041 ggctatataa ttatactatg gatcgcatac aaaattctta gttttatttg gtttcctgta
    38101 tattctattc ttggaaaatg gtcgtatttg attttacttg caaccttgat ctatattttt
    38161 atgcagatag cgtagtatga aggtattaaa tgatataaag gcaaattttg gagtaataac
    38221 ttggtacacg ataatgttga ttttgtgtaa cattacgctt tcgtggattc caagtttttt
    38281 tattaatcag gttaactaca atcaaggaga gacttctttt agtagttctg ttttgttaat
    38341 aagcggtatt tttgctcctt tttttgaaga aacatttatg agagggatat ttcagaattg
    38401 gctcgaaaaa aacataacaa ttccgctttg ggggatatat ggaattgtcg caattgtttt
    38461 cagcgctttg catatgcagt tttactttgt tccttttttc attacaagta ttttattatc
    38521 atatgtttat gatcgatcag aaaaaaagtt agtggttcca tttttaatcc acagtttgta
    38581 taatatattt gtcattgtga taagcaatat ttttagtcat taagatagct aagagctgat
    38641 aaataaggct ttttggctat cttttttgtc tatctggttg ataaaaacta ataggaatgg
    38701 tataatgaat tgtaggctaa ttaagtcttg aataataccg aaggagttga aagaatgaca
    38761 agacgaagta ttaaaatcga gtcagctgaa ccgaatttgc aaacacctcg tacttcaaaa
    38821 attcagaatt caaagaaaaa aactaaaatc tttaagtctg aagatgtggt aagagtagcg
    38881 tggtcggtta aaacgtttga cgaagatcgg tatgtaaaag tttctatgaa tggtcttttt
    38941 gttcagctag gtaattttag tagctttaaa acgattgatg aattatatcc cgaaattaga
    39001 aatattgtgg atagcaaggg tttgatcgtt gcaaaaagaa aatctagaaa ttcaggactt
    39061 gcatattgta aggataaggg aattatgatt cctgcttaat tagctatttg ttctttgaaa
    39121 attgaatatt actcgctctg tggcaatatt gctatgtcgc agaattaagc gtaaggctaa
    39181 caagcaaaat agcaatcgaa gcatactgtt atttctcttg ccatgaggtc gaaaaaaaga
    39241 cgatagcgat agaaatgata aggtttgtat ttcacgccaa aaaatgcata agtcgatcca
    39301 tttgaacctt ctaatgacga ggtggtgaaa atggcattta ttaaaaatat tgacactaaa
    39361 aaattcagcg ctaaaacaag agtgctagac acaagttaac tctttactac aagaaatgta
    39421 gtaaagagtt gttacaattc tttattacat ttctttcaaa ggaagtgtga taatgaaaaa
    39481 aattaaactg attagcgttg ctctgttcag tctaattact ttatcaacgg ctcaccaatc
    39541 ggttgcttca gctgaaggag ccaatagcga aaaagcagtg gtagaagaat ctggtgataa
    39601 aaaagttgca atagacgaca atgttaaaaa tgaagcagaa aaagctgctg ttgttcaagg
    39661 tgcagatggg ttaatgtatg tggtcgttga tgaaattgat ccaaaaaaac tacttgaagc
    39721 cgaatcccct ttgaacaatg aacaagagaa tactggttct gttgaagtag gcgttgataa
    39781 aaagcggcaa gaagaagctg atcaggcaaa cgaaaaagta aaagattttg aagatgttga
    39841 ttataacgat attagttccc ttcaatcagc gttaaataac cctaaatttc cttttttgtc
    39901 tgatgatgac ttaaaagaac aactaagtaa agctaaagat acatttgagt cagaagaatt
    39961 aattaagaaa acaagaggat atttcagtca aaggatttta gaattaggga ataatttaaa
    40021 tgatgtacca caaagagcga aagtgaatcg tcgtgcttta gttagtaccc gtgcttcaaa
    40081 agtaagtgaa acacgaatta acccaccgag tgggtatccg tgggtaattt atcttgttag
    40141 aagtaatgga tatagttaca cagaaaacta tgctatcaga agaactattg atgggaaaga
    40201 agcattttgt ttgcaagcag gtgtgacagc aacgaatact tacacgggtt ccgcaagtgg
    40261 ctatctttct aattctcaaa aggaattgat cagctcgttt ttgtctgctt ataaagaatt
    40321 aggtggatgg gccaataatg attctggaaa acaaagatgg attgcaacgc aaatacttgt
    40381 ttgggaaaac agcaatgaaa agcctaaaag tataactgta acaccaaatt ggaaaagtgg
    40441 ggttactaaa tcaaaaagtg atataacagc tcgtgctaat acgattcggg tgaaacctac
    40501 ttgggatgtg aactctaaaa ctgtgtatga aggagaaaaa gcgacattta cagttaagaa
    40561 cactggggga gagcataaat taaaagttga gtctgtcagt ggtggtggaa aggcgactat
    40621 cagtgggaat aaacttacag tagatacaag tggttctaca ggtacacaaa ttgttgttaa
    40681 aatgacaaag ggatctactg aactaaatcc tacaactgtt tggagtaatg gatcttatca
    40741 aaaacttgtt acaggctctt tttatgttaa tacgtctgtt actgtgaatg taaaacgtaa
    40801 aggtactgta gaggttctaa aaattgattc agaaaccaaa aaaccgttag caggagcgac
    40861 atttagattc agttattcgg gtatcaacaa agatgttact actgactcaa atggtaaagc
    40921 aaaattgact gagcgtttga aagccggaac tgtagtaaag gttaaggaga taaaagctcc
    40981 taacggctat caattagatt caagtgaatt ttcaattacg gttaaagaga atcaaaatgt
    41041 tactactaca agaaccaaca agaggtctac aggttctgta gaaattgaga aactaggcga
    41101 tttagatgga ttaggattgc ctaatgtagt atttacgatt tatagttccg ataacaaagt
    41161 tgtaaaagat aacttaaaaa cagatagtaa tgggaaaata aaagttgatg aactgcaatt
    41221 tggtaaatat tatgcagtag aaaaacaagg tgtcactgga tatgatcctg atggaaaaaa
    41281 atacaatttt gaaataaccc aagatacacc tagtacaaaa ccagctaaag tgaaggtaac
    41341 aaatatttat caagtatcta taagcgaaaa agtatcggat atgaatgaaa gtcaggtatt
    41401 aaaaaatgtt tggtctgctg atccaaacga ttatcttcag tatgacatta caacgggaaa
    41461 tctaaaaaaa tccggagaaa gaaatgtcca aagttttatt atcgaagggt tttacgataa
    41521 taaaaatgta gatgttttaa aaagtgaagt atacgtggga tcaacaaatg tcacaaactc
    41581 ttttgatatt agtaacgata ctggcaacgg aaaagtaact gccaaggcga aggcaacagt
    41641 gttaacgaat aatgattttt ataataagtc ttataattta cggatcacta tgaagataaa
    41701 gaaatctact attgaaaaca ctgaaaagca aaaaccctta acgattgtta aaaatagtaa
    41761 agttactgtc ggttccaaaa tatctaaatc tgctagttcc aacgatgtag aaacagtttt
    41821 atattcaaga aaaattacta tcaaccatat cgatgaaaaa gataaacacc ttttgaaaca
    41881 agatattgat tataaatatg atggggaaac atatgaatat aaacctagaa cagatttgtt
    41941 tgataaagat ggaaacaatt ataaatctga tgtaatccag aaggggaaaa tcaatggaaa
    42001 agatatagtt ttaaccactc cttatcatat tccggcactt tctgtgaatg ttgatcggat
    42061 tcaaattgat acgtctagag ctgttaaaaa tggcttttta cctacagaat tagaactaag
    42121 caaaaaggaa gaatacggaa atgagctgtc aaagatcgta tttaaagtga aaatcacaga
    42181 tatagacagc aataaagttg tttatgataa caactttaaa tacaaagaat ttagtagtca
    42241 cataaaactt aatttgccta cagaatattt aacgaagaat aaaaaggtaa actatagtat
    42301 tagtattcta ttagttgaaa atccagaaaa aaataagttc gatactgaaa caaaagactt
    42361 gaaaacgata ggatatactt cttctgaaaa agttctaaca aacaaagata tgacaaataa
    42421 tcaggtcaac tattcagcag tggttagaac ggtgaaggaa agagcaaata ataatccggt
    42481 taaagagttc cgtgaaagta tcaattataa gtttactgat actaccaaaa cgaaaacagg
    42541 atatggtata tcagttgatt tagagccaac atacaaaaat gaaattggga aacaaataga
    42601 tatgaaagtc tatagctatc caatgaacga attaattgat agtaaaattg acgaaatcta
    42661 tcaaccaatt ggggaaaaag aagcagaagt taagatggat cgcttaaaaa atgaaagtaa
    42721 agttgagtcg ggtactaata ctcaaattct ttcgttccaa tttccacata tgaatgttga
    42781 aagaaaaact ggaaatgtat atgctgacag tcaaaaaaat gatgaatcta ttaagtatga
    42841 gttgatcgat ggtggtagaa aattatatgt gcctatttgg tgcgatttgg ggaaatattc
    42901 tattgctatc aaaagtagta actttggtgg taactatatc tcgttggata tgaatcagag
    42961 catagatgtt tatgcttata tgtatgcaac gatagatagc gaaacgaaaa aggaagatga
    43021 gttgctactt gaacctgtct atccagaaac tactttacca gacaattgga gcaataaaga
    43081 gattgactgg ctgaataaat aaatttgtaa agttattgtc tatctgttag aatttaacaa
    43141 taatatagaa ggtagtgata gaatgagggt acaaagcacc ggctatggaa atagcgtttt
    43201 tgatgtaaaa agagagtttc aagactctcg tagaaagacg agaaagctga ttgcttattt
    43261 cattctatct gctatcgttt tctacattat aaggtttgtc ttggaactaa attcaatggt
    43321 ttttgtggat atggttcata aaatgcttaa tggtgaatca gttgatttca attttgctta
    43381 tatgctagat caagctaaaa gtaatcaagt ggttgcattt ttaatcagtg tttattacct
    43441 cattgaaaag tctgtattct cattaaaaga atttctattg ggtttatttt caagatttgt
    43501 tgatgttgta ggaaaaggaa tagaaaatta gtaaggaggt agttatatgt tagatcaaaa
    43561 tacagaccgt tcgtggtgga tgattggagc ggtaattgta ggagcggtac ttattggcat
    43621 tgctaaagtt gcctttcctg aagtattcaa tacagtaatt gatttcttca aaaatatgat
    43681 tcctacccga ctagggtaaa tcaactaaaa aattcacgca aaaaagcgta ggagagatac
    43741 ataagtgatt atgtatctct ttttttgcag ataggggagt gaaatattgc taagagatat
    43801 gccaaaaatc attcttctta tgtttgttgg tctcttcatg atattaatag ttgcgtatta
    43861 ctctctaaat ggttatacgc aggattcgtc agtaaaaagt attcaagaaa cacttagaac
    43921 taccgcaatt tccaatcgtg atgatagtgt tcgtatagaa aaaggacgat tcattttgat
    43981 gaaagatgac tttgaaaaag attttaaaga aaagtttaac aaagggaaaa atctaaataa
    44041 cgacactacc aagtatgatt ttgattattt atctgatgga aaagacggta ttaaagctat
    44101 taaggtcaaa gtagtaagtg gaaaacaaac atatcaagca acatgtgttt taaacgttgc
    44161 agactagagg tgggaagaaa tgagagtaat agaacaagaa aaaccttctc aaccaacaaa
    44221 aagtcttgta aatctcgtaa aggcgaagaa gtttattaaa gcggaaaata atataagtgt
    44281 aaaggatgaa gatttccttc aaactctttt agaagatgat actgctaact acaatccgcc
    44341 aattgaggaa gcaaggctga atatgattcg tgatgatctt acaagaaatt actcagaaga
    44401 agttttacaa gcactaactg acgatgaaat ccgcaaaaaa cttgtagaag tcgtgttgaa
    44461 agaacacggt agcttattca acaaggataa atctcgtgag gtcgctgagt atgttgttcg
    44521 tgaatgtatc ggaacaggca ttatcgaacg gatcatgaca aatgatgata ttactgatgt
    44581 cggttggaat ggttcacaac taagtattga aacaaatagc aataagtggc tcattgacgg
    44641 tgaaaagcta aatattacac aagaatatat tgaaagggtt atttcaaaat atgcggttgt
    44701 aaacgatcga gattttaata ccggtaatcc aattctggat gggatgttta aaaatgtgcg
    44761 tatatcagct acacacatag ataattcacc tgacggagct acaatgtcta tgcgtgtagc
    44821 aagaccaaaa ctagcattga atactacaaa ttttgagaat tttgctccgg attacatttt
    44881 agatttgttt gaaaaaatgg taaagacgaa agcaaatatt tgcatttcag gagaaactgg
    44941 aacaggtaag acggaattac aaaaattgtt actgagtttt attgataatc atgatcgcat
    45001 gattatgatt gaggatgttc aagaaactca tgcaaaggaa ctattccctg ataaagatat
    45061 ttatgcttgg atcacaactc ccaaaaagac aatatctgat ttagttaagg cttgtttacg
    45121 taacaatcca cgatggatta tcgtatcgga aacacgagga gcagaagcct atgaaatgtt
    45181 acaagcaatc ttgtccggtc accatgttat tacaacactt cacgcaatta atgctcgtgc
    45241 aattccaagg cgatttgtca atatgtgtgc gagtggttac aacataaatg aaacagcggt
    45301 ggaagaagat attaaccgtt attttgattt tggagttcat attaagaagg ttgtgattaa
    45361 tggaaaagtt attcgatacc taaatgaaat cgttgaattt tcagatactg gaacaacaac
    45421 agttttcaag caaaaactta ttaacggtga gtttataagg gaaaatggtg cattgtcaga
    45481 tgattttaaa gaaagaatgg ccgaaaaatt tatctcgttt gaatttccgg aagataagat
    45541 agaaaaagaa gatagcgaaa ctaatgaatt aaatagcgaa aattcagagg ttgaggtgat
    45601 cacatgaagg tacaagcagt aacaaagaaa aagggaatca tggatgtttc agaattaaat
    45661 gcgtatcaag ttgcgtatgg aaaaccactt gattatcgag attatctgaa ttatgtagga
    45721 ttaccttgca tattcatggg tgtattctca ttttctcttt gctatttttg gtgggtgggt
    45781 ttgatttgtg gagtgattgg cgctgtgtat ggctataagg ttgttatgcc aaaaagtgta
    45841 aagcgatctt atgaattgaa gagtttgaga gaaagaaata agttcattaa caatatgact
    45901 caaatcttga cggatgaaag taagacagta acgaaagcat tagcaatggc aaaaacccga
    45961 accaaaggag aattaagaga agatattcaa attttagagt ctcgattaca aggagctgac
    46021 aagtttcaaa tagctgaagc atttagagaa ataaacgaaa agtatgaacg tgacgttgtg
    46081 ttcactcaat attttgagca acttgaaaca gcaatttatg aaggtcgaaa taatgctgat
    46141 acgatgaagc agattaaaag ttatcacaat gatgtcaagc taaaaacaga gagcttttta
    46201 aaaatcaaag atgggtatct tcaagattta aaacagatgc tgttcatagt agcaatattt
    46261 atcggagcaa ttacattttc atttggattt tcaacatttt ttgatggttt tgctcgatca
    46321 ccgatcggtt ggggatttgg cggtgtttat ttcattatca tggtacgctt catgaagtca
    46381 ttcttcatgc attactttga tgatgaaatt atgagcttgg gggtgagtaa ttagtgaaag
    46441 tagctaatgt acaaaaaaaa gcttccaaga aagcaagaat aattaagcaa gagaataaat
    46501 caatctggct taaaatgtta gacgaaaatt attataagaa attacgtgaa atgaataact
    46561 caaatgtctc aatcataaag tggcagaagc aaaggtttct taaatcatgg atattcgttg
    46621 gtgttggatt agtcatgtat ttgcttttag ggaaagaatg gtattaccta gcaggtggta
    46681 tagctacagc aatagggttc tacattatac aagcaaagtc aataaacaat atttataatc
    46741 aatttcgttt tgaacgacac ttgcaattta gtaaatttac aagattactt attccgtatt
    46801 taaaacagaa aaaagacggt ggtaatcttt atggtgtctt tgcaaaaatt gttaaacgtt
    46861 tagattatga cacggataag caactattga tgaaattaat gcaggatatg acagatcgtc
    46921 ctaatgatat tcagcctttt gtggaatatg cagagcaaac ttcaggaaca gacatgagtg
    46981 tgttgtttat gcaaacaata tatgatattc gtcagggttc agcagattta gatgtaattg
    47041 aagaacttga tcgtttagct tctgaagaat tgatggccgg tattgattca attattgatt
    47101 tcaaaagtcg gagattcgtt ttttatccga caaaaatagc tatgacttcc tttattcttg
    47161 tggtcgggtt tgctgtaggt gttctttttc ttaacttgaa agacataaac tttggtcaat
    47221 aaaaggaagg aagaaaatat atgcaagaac aatcaataat ggagatagtc aaatggttta
    47281 taggtcttgt aataatcatg gtgatggtgt ctgttggttt attttgctac cagcttcaag
    47341 atgtgaatag ttttaaacaa caggtaaatt atcaaataga aagaaggggc ggtttaacag
    47401 ctgaagcatt aaaatcatta aaagaatact cagataaaaa ttatcaagga cgctataaaa
    47461 tcgaaagtaa tcttttgaat caaaaagtag aatatggcca agctgttgat tacactgtta
    47521 aagctacaat cccaattcaa attttaccat taccggatgt tacgctttca tttgaaggaa
    47581 atagtgtgtc acaaatcaga taagaggtgg taatagtgca agaaaataat actgcaagaa
    47641 ttctttttct aataggcgga atggtaatct tatcactagt tatcactgga atagctacac
    47701 attgggaaga aatcaaatcg ctgattgaaa cgttcttcca aaataagatg atacgaatgt
    47761 gaggggaaat agtatggatg aaactgtaga acaagtaaat aagccaaagc gacaaagaaa
    47821 gaaaaaggtt gatcctttta ttgaaatgac ggacaaaatt attcagaaaa aaggattaac
    47881 tcctagagag ttcgatcaga cagcaattag aaaagaacgg caaaagcttt atgatgaatt
    47941 cgttaaagaa aatattggat tacttttaga ctaaatataa ggggcgcttt aatatgaaag
    48001 aaattatgat aactaaatta gcaatgaata tgccaaaaat ggactttttc gataatgtat
    48061 caaagtttat gtcaaatact acgtctaaac tgcaagggat ttctctttcg gtaattattt
    48121 tctgtgttgt agtaacagga atgatgtttc tttttgggga agggccaagt agaacagcta
    48181 aaaagtggct gacgtatatt gttatcggag ctttaatcat ttttggtgct tcaacaatcg
    48241 gccaaacaat aaaatctgtt ggtggattct aaaattaata ggaataaagg agcattgaga
    48301 ttataatgct cctttttaat tgaaaaaaat gaagtagaga aggagaaaag tagatgaaac
    48361 aacgttttga atactcattt gaagatgtag aatctaaatt attacgaaaa gggaaaggct
    48421 taaaaaagaa aaacaaatat tcattaaaac aagcagaagg cttgattgct tgggtacaac
    48481 acaactggcc aaataaaaca gaagttactg taatgtgcaa agataccgaa gatggtgctg
    48541 tatatgcgtt tgatgggatt gaaattacac cgtttggtat ggggggagaa ccaattgcat
    48601 tgtttcctat tattgaggat caaattctac aggctccgga taatatgatt aatgatccta
    48661 ataaattgat tctagacatt aatagccaat atcagtcaga gccagatata gcagaagcga
    48721 aacaagaaag taagtccaaa aaagggttaa accttccaag cgttcttcca aatgtcaaat
    48781 taccttttgg aaagaaaaag catttggagg aaacacctga aagtgatcaa ttttttgaag
    48841 aatacgaaac agaaagtaca ccagaattag atgaggagct tgaattagac gaagagtctg
    48901 aagaagttga agcggaaatc gaaactgatc ttgaacaagc tgaggaaaca atgttagatg
    48961 aaactgaatc attcgatttt gaggaagaaa ttgacatgac tacaagtagt catgagagtg
    49021 ttgctattaa ttctcaaact tctgtgcctg catattcaca aaacacttat catagtgagc
    49081 aaaattttgc gttagccagt tctattccac aaacggttcg acaaattaaa aagcatgaaa
    49141 cagtccaatt aactccatta caagaatatt gtgatttagg agaagaaatt gaatcctcta
    49201 ttgtatcaac aactaataaa cttcagccag aaaatctgtt taaatttatt ggtataccag
    49261 ctgtaaaaaa tacgcgatta gatgaataca gaatcaatca tgccatgcaa cgattagctg
    49321 aggttaagtt tgaaaactta cgagaacatt attctcgtca agttaattca atgatagcag
    49381 aagctttgga caaactacga gaagctattg atgtggcatg gaaaaagcca tatgaccttg
    49441 aggtgaaaaa acggtatgct gacattttag aagaaatgga aactaattct aactccacat
    49501 tagaggagtt tgtaaaacgg caatatgata tggtggatga aaaaatcaaa aagtttgaaa
    49561 cagaacaaga aattgcactt caaaacttta ttgctaatca agaagctgaa aaagcagtct
    49621 ttattgctaa cgagaaagag cgtgcgcaaa acttaattga tacaaaatca gagactatta
    49681 caagtgaatt agaaaaacga agagaagaat ttatagatga agaaatgtac aaaatgaaaa
    49741 ttgacatgaa tcaaaatcta ttcgatggaa aaagattaat taagcaagag ttagcagttg
    49801 aactagctaa tgccaatgaa gatgtatggg aaaatgcact gaaatttatt actgaaattc
    49861 aaaatgaaat caacgaaaag acaccacttt gggcttctga aattaaagag actaacattt
    49921 tagaacagca agcacaccaa atgcaaaaag aacaacagga actaaaactg aaagaagagt
    49981 cattggctat taaacgttta gaagccgaaa caaatctgaa acgattagaa gaacttgatc
    50041 acgaaaataa aatgttaaaa atcaagttgg aaacagcgaa ttctaaaaat gaactctatg
    50101 aatatgagaa ggaaacacag caaaaagttg tggggtcaac tcaagcaaga aatttgagta
    50161 cgttgggatt gtttagaaaa taatcaacta aggagactat aaatgagtgt atttaataat
    50221 aaaaacatgg tgtcaggtta tgtgaatcat gttgcggtat atgatggtgg atcttttaag
    50281 acaatgtttg ttcgtttagc tgtttttaac tatatgaaag aggataaagc acagtacgaa
    50341 gtaatcaatg cacaaatatc ttgtagtgta gatcaacaag gtaggctaac gactacaagt
    50401 agtcacccga aagcgctgtt gaataaaatg cttttcgata aactagataa aaatttagtg
    50461 gaaggaatga aagtaacttt gcatggtagt ttgcgtggta gtgatcaagt tttcttaagt
    50521 ggccggtggc gaaattttaa agatttatct tcagctgaaa aagaagaata taagtctttg
    50581 ccaaaaggtc aattaataac tagaaccaat tcttttattt atatagagga agtaaacatg
    50641 ccgagcaagg aaggtttttt gaaacgtaaa gaggaactac taacagaaaa tcaaatgaat
    50701 gaagtaaaag ttacagagcc tagtcaagct gatcaaatgg aaacagatga taatgaccct
    50761 gtcgataatg aaggtgttgt agttgaaaca gtagaagctg aaattataga accgcaagaa
    50821 ggtactaaaa tctctcgtag aaatacagtg tcctacgtac gattcgagga catgatttac
    50881 cctgaagcca ctatggtgga agaagattac cctttctaag ttatacgagg tgaaaaatga
    50941 gacttataaa tgataagaaa cctgacaata agttaaaagt attgttagaa aaaggaaata
    51001 aaattgaaat cggggatatg atgtggttta aaggaaaaaa taaatatcat agcggaaaga
    51061 ttgaagggga agatttacct gaaaatgtaa aagatatggt taatcttccg ttattgttgc
    51121 taggaaccgt tctaggactc tttgcgaatc tgaccgtaaa ctacttaatc aatttttttg
    51181 cagctttcct gaaggctctg agtgtttctg agaaaggatt aatgagcatt gaatttgatc
    51241 atggattcca tatgggctat ctaaaaccat ttaccagtgg aactcaatat tttatgattt
    51301 cgatagtcgt atgtatcgca ataacatttc tagtttataa caaattagat catgaaagtg
    51361 acagaaatct tgtatacggc caaaagggtg attcgagatt tgcgacagta aaggaaattc
    51421 aaaatgaata tcatgaaatt cctgaaaaga aagttcgctt tgaaggaatg ggtggcgtac
    51481 caatctcaca ttataaagat aagtattaca ttgaccgtga tacagtcaat acagttgttt
    51541 taggagcttc tcgttcaggg aaaggcgaaa cctttgttgt tccaacaata gataatctct
    51601 caagagctaa agaacagagc aatatggttg taaatgatcc aaaaggagag ctgttttcag
    51661 caagtaaaga aacattggaa aaaagaggtt atcgagtatt agttctaaat attgatgatc
    51721 cgttacaatc aatgagtttc aatcccttgc aacttgtaat tgattcttgg gcgaataagg
    51781 attatcacga agcaagtaaa cgtgctaata cgcttacgtc tatgcttttc gcttccggta
    51841 tgggaacgga aaacgaattc ttttacaaat cagctaaaaa cgctgtcaat gcaataattt
    51901 taacaattgt agaacattgt tttgataata attgtattga aaaaattact atgtataatg
    51961 ttgcgcaaat gttgaatgaa ctaggaagtt tgttttatac cgatcctaag aatccaaata
    52021 aagagaaaaa tgccttggat gaatacttca atacattacc acaaggaaat caagccaaaa
    52081 ttcaatatgg ttcaacttcc tttgcaggag ataaagccaa aggctcaatt ctttctactg
    52141 cttctcaagg aattgaaatg tttaccagtg atttgtttgg aaaactgaca agtaaaatgt
    52201 caattgactt aaaagagatt ggtttcccga agtcacttca attcaagcta aacactgaaa
    52261 tggttggaaa aagagtaaaa atctcatttt taagaaaaac agatggcaaa acaaagataa
    52321 tcaatgaata tcgagtaaaa gttaaagcgt taggtttatg tgtgttaaat tttgacgaaa
    52381 gcctacgaga tggcgatttt ctcgaaattc gttatgaaac agaaaaagaa aaattcagag
    52441 catggtatag ccttgaattt cctaaaaggg attcagaaga tcacgataca acagtgatca
    52501 cgaaaaaatt caaatcagga aaaagattcc ttgatttaga aatcaattca gtcgttctta
    52561 aatatactga tcaacctact gctgttttta tggtaatacc agactacgat ccaagtaatc
    52621 acgtaatcgg aagtattttc attagtcagc tatatacgga gcttgcaacg aactgtaagg
    52681 aaactcctag taaaaagtgt ttccgaagag ttcattttct attagatgaa tttggtaata
    52741 tgccagcaat tgacaatatg gatgggatta tgaccgtatg tttaggacga aatatactct
    52801 ttgacttggt tatccagtct tatagtcagt tagaaagtcg atacgataaa ggcgcaaaaa
    52861 ttatcaagga aaactgccaa aatcatattc tgatcatgtc aaatgatgac gaaacaatag
    52921 aagaaatttc aaagaagtgt ggaaataaaa cgatcatagg aaaaagtgct agttcaaaaa
    52981 gaatggatac tgatagttcg gtcacttctt cagcagatca agaaagaatt attacttttg
    53041 aacgtgcgag tcagctaatt gaaggtgaac aagtaatttt gcgtaatctt catagacaag
    53101 ataaaaaccg aaaaaaaatc agaccttttc caatctttaa tacaaaagca actaatatgc
    53161 cgtatcggta tcagttttta aatgatgatt ttgacacacg aaaagatatt aacgaaattg
    53221 atatagcgtg tgatcacatt aatctatctt tagctgataa tcaaattcca tttgcaagct
    53281 ttattcgtga tttaaaaaca agaatggagt attcaattac aaatgaaata ctaatatcag
    53341 aagatgatta tgaagaatat cgtagtttgc ccaattcaca acaaacgctt gatgaagaga
    53401 agctaattgg attggtgaaa aaatcaaatt ttgagaaaaa atcagaagtt gagctatcag
    53461 aagatgattt caaactaact acctatcttt tagattgtca aaaacataca aaaaaactac
    53521 atgatttaat gtatgagaaa gatagatttg ctgatgataa atcccttgac ctagtgctta
    53581 aaacgctaaa tgaaagatat ttttcttcag agctggtaca agaatttcaa aaaggacggg
    53641 tgactgctca aaatatcaag cagtccaaaa aacagctagc agggataatc aatgttttag
    53701 gacaggtata tatttatgcc gaaaacaata tattcaaaac gcatattata gccgttaaga
    53761 acgcactaac aaaattaagt aaggctatct gaatatgaag gatgtgaaaa tttttgacag
    53821 aagcaatttt aacaagagct aatagaatta aaataagatg gcctatggta cttttactag
    53881 cgacaatatt gctattcgtt atggttccca caacagcgta cgcgtggccg tgggattcag
    53941 atgataaaga agcattagat tttttgctga agtatcaaga ctatttacag tttacaaaag
    54001 gtggctttgg aagtattgtc atgcaaacct tttcgtgggg aataattcga tttgcgtatt
    54061 ggatttcaaa aactatcgaa gggttgatac cgcaagcgtt aagtttattt agctttgtgg
    54121 aaagtgctgg attgaacaaa gtctatcagt cagttttaaa tacagttgtt gttgggttaa
    54181 tggtactctc actagttatt attggatata aaatggtgac tggaaaaggg actttagact
    54241 taaaatcggt tggaatgaac gtagttatgt cagtagcatt aatattatta atgcctacta
    54301 tgatttcaag tggaattacg tttgctaaaa ccttttataa tgacgcaact agtattaaca
    54361 atcaagatgg cggtgtggca tggtctctta ttgaagatgg agtgacagat ttagtatata
    54421 tcaataaaaa tgacgcttat aatcgcatgg ggaaagaagg ccaaaagaaa aatgcattaa
    54481 aagagaaaga tttttttact tatgatttta gtgaagtaat aacgactgac acagcgaagg
    54541 aattagaagc agataatcct aaagcggagt atttaaagat gaaattgact cctgatggtg
    54601 atggaaatat gcaagccgta aaaattgatg atggatggat ggccattttt tctgatagct
    54661 ttaagggagg ttattataga tacacgaaaa acacgtttgc gttaacgttg ggcttaattg
    54721 cattggcaat agcgtatgtt tttagtgctt tcattataat tacagcaata ctcgagttag
    54781 catttaaacg tgtactcgga gttttagtat ttgctactga cttggaaaca ggccaacgat
    54841 caaagcaagt tttgtcagat attttacagt gttatttgac agtcggattt caagggttcg
    54901 gattgtctat gtttgcaatg tttgttaatt atctcggaac aggtagcggt tcttctgtaa
    54961 atccttttat aaaagcagta gcgtacgtct gcgctgttat tgtgctgata aagggtagtg
    55021 gaacagtcat gcgttacttt ggcgtggata ttggtttaaa agaaggctat ggccagcttg
    55081 cttcagcatt tggcatggga gctatgctgt ttcgtaaagg aacaggtggt atgaaccgat
    55141 ctaaaggtgg gaaaaatagt ggtggaaacg atcaagcgga aggggaagaa cgaaaacctg
    55201 aacgcaactt tggtcaatca ctttctcaaa aagctaataa aacggggaaa acacttgggt
    55261 atgctcgtga acgaggatta tcaggcttag cttcggatgg agctacaatg gccagtgaaa
    55321 gagcaacaaa accatttaag agtatgcgtg atatggctaa tgataccaag aataaattca
    55381 aagaaggttt agatgatggt accgtgtccg ctattaataa aaatagcaaa cctatagtag
    55441 ctaaaaacaa agaagatgaa actggaaaat atgctgattc tatgccaaca cgtctttcgg
    55501 atagaaaaga tggcgctaaa gttgaaaacg ctgatagaat tatgtctagt tctgaacgta
    55561 tgcgtgaagc aatgaaaaat aatgctgaat caaatattaa tcctgcaagt gcaattcagc
    55621 aaaaagttaa acaagatatt gaagaacgta aaaatgctat gcacggtcaa gctaagtctg
    55681 ctgaagaatt aattaatcag aagcgacagg aagcaaaata cacgcctgaa gcaatgaatc
    55741 gtgaggaaat gttaaggaaa cgtgtagaag ggcgtacagg cgctaatatg gacgaaaaag
    55801 aagcgttgac taaagaaaga atccaagaag ctaaaaattc aaataccgga ttagaaaaat
    55861 cagtcaaaga aaatctacaa aattctacaa gtagtcaaga taggcgctca gtagatgttc
    55921 gagaaaactt aca
//
LOCUS       DANKAY010000006        53878 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000006 DANKAY010000000
VERSION     DANKAY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..344
                     /locus_tag="PWY98_000364"
     CDS             <1..344
                     /locus_tag="PWY98_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036149.1"
                     /translation="YRIFASKLSSILSDKINVKKSFFKKETPVEKISVELLKEMRNLM
                     QEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLV
                     EDADLSYLPLK"
     gene            complement(425..994)
                     /gene="thiT"
                     /locus_tag="PWY98_000365"
     CDS             complement(425..994)
                     /gene="thiT"
                     /locus_tag="PWY98_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2036150.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1060..1162)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1168..2139)
                     /locus_tag="PWY98_000366"
     CDS             complement(1168..2139)
                     /locus_tag="PWY98_000366"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2036151.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2233..3606)
                     /gene="glmU"
                     /locus_tag="PWY98_000367"
     CDS             complement(2233..3606)
                     /gene="glmU"
                     /locus_tag="PWY98_000367"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2036152.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4109..4927)
                     /gene="purR"
                     /locus_tag="PWY98_000368"
     CDS             complement(4109..4927)
                     /gene="purR"
                     /locus_tag="PWY98_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2036153.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5094..5918)
                     /locus_tag="PWY98_000369"
     CDS             complement(5094..5918)
                     /locus_tag="PWY98_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2036154.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5891..6571)
                     /locus_tag="PWY98_000370"
     CDS             complement(5891..6571)
                     /locus_tag="PWY98_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2036155.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6594..7532)
                     /locus_tag="PWY98_000371"
     CDS             complement(6594..7532)
                     /locus_tag="PWY98_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2036156.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7777..8640)
                     /locus_tag="PWY98_000372"
     CDS             complement(7777..8640)
                     /locus_tag="PWY98_000372"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2036157.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(8782..11355)
                     /gene="mprF"
                     /locus_tag="PWY98_000373"
     CDS             complement(8782..11355)
                     /gene="mprF"
                     /locus_tag="PWY98_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2036158.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11496..12302
                     /gene="proC"
                     /locus_tag="PWY98_000374"
     CDS             11496..12302
                     /gene="proC"
                     /locus_tag="PWY98_000374"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2036159.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12381..13937)
                     /gene="rny"
                     /locus_tag="PWY98_000375"
     CDS             complement(12381..13937)
                     /gene="rny"
                     /locus_tag="PWY98_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2036160.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14348..15397)
                     /gene="recA"
                     /locus_tag="PWY98_000376"
     CDS             complement(14348..15397)
                     /gene="recA"
                     /locus_tag="PWY98_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2036161.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15506..16747)
                     /locus_tag="PWY98_000377"
     CDS             complement(15506..16747)
                     /locus_tag="PWY98_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2036162.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16987..17565)
                     /gene="pgsA"
                     /locus_tag="PWY98_000378"
     CDS             complement(16987..17565)
                     /gene="pgsA"
                     /locus_tag="PWY98_000378"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2036163.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17774..18697)
                     /locus_tag="PWY98_000379"
     CDS             complement(17774..18697)
                     /locus_tag="PWY98_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036164.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19436..20722)
                     /locus_tag="PWY98_000380"
     CDS             complement(19436..20722)
                     /locus_tag="PWY98_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2036165.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20715..21983)
                     /locus_tag="PWY98_000381"
     CDS             complement(20715..21983)
                     /locus_tag="PWY98_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2036166.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22235..22696
                     /gene="mscL"
                     /locus_tag="PWY98_000382"
     CDS             22235..22696
                     /gene="mscL"
                     /locus_tag="PWY98_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2036167.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22755..23201)
                     /locus_tag="PWY98_000383"
     CDS             complement(22755..23201)
                     /locus_tag="PWY98_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2036168.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23294..23539)
                     /locus_tag="PWY98_000384"
     CDS             complement(23294..23539)
                     /locus_tag="PWY98_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2036169.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23654..24232)
                     /locus_tag="PWY98_000385"
     CDS             complement(23654..24232)
                     /locus_tag="PWY98_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2036170.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24309..24839)
                     /locus_tag="PWY98_000386"
     CDS             complement(24309..24839)
                     /locus_tag="PWY98_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2036171.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(24843..25703)
                     /gene="rlmB"
                     /locus_tag="PWY98_000387"
     CDS             complement(24843..25703)
                     /gene="rlmB"
                     /locus_tag="PWY98_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2036172.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(25696..26091)
                     /locus_tag="PWY98_000388"
     CDS             complement(25696..26091)
                     /locus_tag="PWY98_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2036173.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26088..27500)
                     /locus_tag="PWY98_000389"
     CDS             complement(26088..27500)
                     /locus_tag="PWY98_000389"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2036174.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27497..28033)
                     /gene="cysE"
                     /locus_tag="PWY98_000390"
     CDS             complement(27497..28033)
                     /gene="cysE"
                     /locus_tag="PWY98_000390"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2036175.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(28119..28358)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28355..29815)
                     /locus_tag="PWY98_000391"
     CDS             complement(28355..29815)
                     /locus_tag="PWY98_000391"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2036176.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(29985..31142)
                     /locus_tag="PWY98_000392"
     CDS             complement(29985..31142)
                     /locus_tag="PWY98_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2036177.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31176..32546)
                     /gene="radA"
                     /locus_tag="PWY98_000393"
     CDS             complement(31176..32546)
                     /gene="radA"
                     /locus_tag="PWY98_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2036178.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(32609..33136)
                     /locus_tag="PWY98_000394"
     CDS             complement(32609..33136)
                     /locus_tag="PWY98_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2036179.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33366..34172
                     /gene="proB"
                     /locus_tag="PWY98_000395"
     CDS             33366..34172
                     /gene="proB"
                     /locus_tag="PWY98_000395"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2036180.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34169..35416
                     /locus_tag="PWY98_000396"
     CDS             34169..35416
                     /locus_tag="PWY98_000396"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2036181.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35492..35836
                     /locus_tag="PWY98_000397"
     CDS             35492..35836
                     /locus_tag="PWY98_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036182.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(35953..36279)
                     /locus_tag="PWY98_000398"
     CDS             complement(35953..36279)
                     /locus_tag="PWY98_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036183.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(36894..38096)
                     /locus_tag="PWY98_000399"
     CDS             complement(36894..38096)
                     /locus_tag="PWY98_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2036184.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38213..38728
                     /locus_tag="PWY98_000400"
     CDS             38213..38728
                     /locus_tag="PWY98_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2036185.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(38927..39373)
                     /locus_tag="PWY98_000401"
     CDS             complement(38927..39373)
                     /locus_tag="PWY98_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2036186.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39420..40898)
                     /gene="msr(C)"
                     /locus_tag="PWY98_000402"
     CDS             complement(39420..40898)
                     /gene="msr(C)"
                     /locus_tag="PWY98_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2036187.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41252..41881)
                     /locus_tag="PWY98_000403"
     CDS             complement(41252..41881)
                     /locus_tag="PWY98_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2036188.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(41865..42329)
                     /locus_tag="PWY98_000404"
     CDS             complement(41865..42329)
                     /locus_tag="PWY98_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2036189.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(42816..44084)
                     /locus_tag="PWY98_000405"
     CDS             complement(42816..44084)
                     /locus_tag="PWY98_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2036190.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44315..45052)
                     /locus_tag="PWY98_000406"
     CDS             complement(44315..45052)
                     /locus_tag="PWY98_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2036191.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(45119..45688)
                     /locus_tag="PWY98_000407"
     CDS             complement(45119..45688)
                     /locus_tag="PWY98_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2036192.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            45816..46688
                     /locus_tag="PWY98_000408"
     CDS             45816..46688
                     /locus_tag="PWY98_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2036193.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            47030..47902
                     /locus_tag="PWY98_000409"
     CDS             47030..47902
                     /locus_tag="PWY98_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2036194.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            47899..49857
                     /locus_tag="PWY98_000410"
     CDS             47899..49857
                     /locus_tag="PWY98_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2036195.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(50189..51040)
                     /locus_tag="PWY98_000411"
     CDS             complement(50189..51040)
                     /locus_tag="PWY98_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2036196.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            51163..51630
                     /locus_tag="PWY98_000412"
     CDS             51163..51630
                     /locus_tag="PWY98_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2036197.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(51893..52375)
                     /locus_tag="PWY98_000413"
     CDS             complement(51893..52375)
                     /locus_tag="PWY98_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2036198.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(52475..53338)
                     /locus_tag="PWY98_000414"
     CDS             complement(52475..53338)
                     /locus_tag="PWY98_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2036199.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        15393 a        11533 c         9593 g        17359 t
ORIGIN      
        1 tttataggat ttttgctagt aaattaagtt cgatcttgtc agataagatc aatgtaaaaa
       61 aatctttttt caaaaaagaa acacctgttg aaaaaatcag tgtggaatta ttgaaagaaa
      121 tgcgtaattt gatgcaggaa gggatcattt tttcttttga tcaagttggt ttgatcgatg
      181 gtaaatatcg tatcccaatc tatgtgattg aagattatat ttggaaatta aaaggcaccc
      241 ggtatcgaga agaggaaatc atcgctactt taatctactc tgtcaccgat cgacactggc
      301 aattggtaga agatgccgat ctttcttatc tgccattgaa gtagatctat ttcttatcaa
      361 aaaataaaaa ggaaacaaga aactaggaca tgattctgtc tcaagtttct tgcttctttt
      421 ttatttacag ctcctgtcgt ttatctggca caaaaatcga aggagtcgtt tgttggatcc
      481 ctaataaaac gatgatcgtt acaatagctg tactcaaccc gctggcacca ttcattacga
      541 aagaaaagag ccaaggattc atcccccata gtgcatacgc tccccagaaa accactcctg
      601 caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa tgcaccaaac acgatgcttt
      661 ttctaatttg tttcgtatta tcgtctacat agcctttttg gacatttgaa gccatgagcc
      721 ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat caacacttga acgacactta
      781 aataataaac ttgtcccaac ggaaaatgta ggatgcccca aatgaaacct gccagcatcc
      841 ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg aatctgccct aatgaaatag
      901 aaaaactgct gccgatctgg atcggaatca acgacaacac catcgctaac gctgcaacaa
      961 tcgttccttc tacccaaact ctattttttc ccataaaaaa acttcctcct ttgaataaaa
     1021 aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac agctgtttat tcacaattcc
     1081 tacgctcgca taaccgatac aggtacgaag ggtttagaaa tagattcaat ctcagcccaa
     1141 ttggactccc ctttgtgagt ttatttatta tttatttttt gtttcaaaca atggactaac
     1201 tggtttgttt tcatggatac gtttgattgc atctcccatt agtccgccaa cacttacttc
     1261 atcgattttg tcgattttac gatcttctgg aagatagatc gagtctgtaa caactagacg
     1321 ctcgatagct gaatcttcta ttctttgtaa agcagggccg gataatactg gatgtgtaca
     1381 tgaagcatac acactagttg caccagcttc tttcaaagca ttggcagcca atgtgatcgt
     1441 acctgctgta tcgatcatat catcgatcag tacacaagtt ttgcctttga cttgtccaat
     1501 gatattcatt acttctgcaa catttgcttt tggacggcgt ttgtctatga tcgcaatcgg
     1561 agatttcaaa aattccgcta atttacgtgc ccgtgtgaca ccaccatggt ctggtgaaac
     1621 aacgacaaca tcatcgcctt tgattccttt ttctaagaaa taattggcaa taagtggcgc
     1681 acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg atttggactg catgaaggtc
     1741 caaagtcagc attcttgttg caccagcttt ttgaatcatg tttgctacta atttcgctgt
     1801 gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca tagccataat aaggcatgac
     1861 tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca atcatgatca gcaactccat
     1921 caagttatca ttgactgggc tgctcgttga ttgaataaca tagacatgcg atccacgaat
     1981 actttcttca atgtttactt gaatttctcc atcactaaat tgagtaacag atgattttcc
     2041 taactcgacg ccgactgctg ctgcaatttt ctctgctaat gggcgattgg aatttaacgc
     2101 aaaaattttt aatcttgggt caaagtaatg ttttgacatg gggacctcca ctttcttttt
     2161 cacaactcta gaattctacc taataaatag tagtcatatt ttttaaataa atcaagtgat
     2221 tttgcaaaag tcttagttta gataagggag tttttttgca tatccttctt tattgacttg
     2281 tcttgctctc gcaattgcca gcgcatattc tggaatattg tctgtaattg ttgacccggc
     2341 agcaatcgaa ctgtttttag ctacctcgac aggtccgatg atattcgtgc tagaaccaat
     2401 aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt ccatcatagt tgacgaagac
     2461 aacaccacat ccgacattga tttcttctcc cagcgtagca tcaccaacat aagtcaagtg
     2521 accgacttta gttcctttgc cgatttgagc atttttcact tccacaaagt ttccgatatg
     2581 gacgttttcg ccaatttcag cttttggtcg taaatgtgca taaggtccca catctgcatg
     2641 tttcttgaca tggctgcttt caatgactga gttttcaatg acgacgtgat cctcaatcct
     2701 actgtctacg atttttgaat gtgacccgat gacacaatca gaaccaatga ctgtatttcc
     2761 ttggatttgt acacctgctt cgatcaatgt gtctggaccg atttctacgc ctgcatcgat
     2821 gtatgtcgtt gctgaatcga caaagcttac gccattgacc atatgcatct ggttaattcg
     2881 ctttctcatg atctcatttg ctttagctaa cgcgatccga tcattgactc ccattgattc
     2941 ttcaaaatca tccgtttgat aagcagcaac tgtatggcct tcttctttta agatttcgat
     3001 gatatctgtt agatagtatt caccttgagc attgtttgtt ccaactttgc ttaacgcatc
     3061 gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg atttcttgta caagagcttc
     3121 ttctggtgtt gcatcttttt gttccacgat tttttctacg atcccaatat ggtcacggat
     3181 gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc aagatcgttg cacttgcatt
     3241 ttttccttgg tgatattcaa aaagattgtt cagcgtttca gtagtcaata atggtgtatc
     3301 gccactgata actaacgttg ttcctttttt tccttccaag aaagaagcag cttgcaaaac
     3361 agcatgtcct gttcccaatt gttctgcttg taacgcatat ttactgcgtt ctcctagttg
     3421 tgctttaact tgttctgctc cgtgtccaac gatcgtaatc acttcatctg gtttcgtttc
     3481 actgacccga ttaatgatat gttctaccat aggtttacca gaaacgggat gtaaaacttt
     3541 atataatttt gacttcatgc gggtaccttt ccctgccgcc aaaataattg cataacgtgc
     3601 gtccaactgc gtcactccta aatatagttt gctgatttca gtttatccca gtatacccat
     3661 tttttcaaca aaacaagtga ttttctttta tcccagcgtt ttctggataa aaaaatagat
     3721 agaccataac gggcctatct atttggtaac cggtgctctc ctgccaactc ctcggtcaca
     3781 agtcacacta ctcataaaac atgaaaagct gttttttgaa atcgtttctt gtgacttaga
     3841 gttaaacggt aggcttacaa cctcttcatt aaacgttttt caatcagctg accttcggta
     3901 ttagctcaat aaacggcaaa atatgaggag caatttttcc gtttattgag ctaatactca
     3961 ggacaggacg ctgatttcac aacctctaga taaacggtgt tcaaaagcca gtttctcggt
     4021 attaagccaa atttatgaaa aaatgtggaa agccattttc acaaaatttt tcttactact
     4081 cgaaactaag cggctttttc acaacctctt attctgaaaa ataatttcct ggcacaaccg
     4141 taatattttt cgtttgagta tctacatctt tgacataaag cagtgacgta taatcatcga
     4201 tggcgcgatg accgttgaat tttgattcgg caaatacagt gatgccaaca agttctgatt
     4261 cgaattcttc aatcaagctc ttcattccgt tgactgttcc gccgcctttc atgaaatcat
     4321 ccactacaag gacacgtgaa ccacgtttca aacttctctt agacagttcc atcttttcaa
     4381 ttctttcaga agatccagac acatagttga cacttactgt tgatccttct gttatttttg
     4441 agtcccggcg tacaataacg aatggcgcat tcagataata ggacacagct tgggcaatag
     4501 gtacgccttt tgtggctaca gtcattacag catcgatttt ttcaccaagg tatttagaag
     4561 caatgattct tccaacttga cgcagcagtt caggttctcc taacaaatca gagagataaa
     4621 catatcctcc tggaagcaga cggtcttgtt ctgatagacg ttctgctaga gattcaatat
     4681 attttttcgc ctcttcatat gaaatttcag ggatgaacaa tacaccacct gcagcacccg
     4741 ggattgtttc caaggttcca taccctcgtt ctttaaatgt tttcttgatg attcccaaat
     4801 cttcactgat agaagattta gcagattcat aacgttcggc aaagcttgtt aaggaaatca
     4861 attcatgcgg gtgattcaat aaatactgtg tcatgtcaac tagacgttca ctgcgacgta
     4921 ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt ttacattata taagtttttc
     4981 ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag ttcggtattt attaaaataa
     5041 aaagaagcct ataaaaaatt tttcgttttt ataaggcttc ttttctttat aatttagtta
     5101 ttttttcttc tgaaggcaac caatattcgt ttgatgatat ttacaatcag gaacaaagca
     5161 ataaagacta atgtgattgt tgctcctgga ggtgtatcta gataaaacga gctagttagt
     5221 cctgacagca tccctagaaa tcctacacaa acactgatag caatcacgac attgaagctt
     5281 ttgcctaatc gcataccgat tgctgcggga agaaccatga ttgcggatat caaaagtgca
     5341 ccggcaatcg ggatcatcac agcaatagcc acaccagtaa tcacattgaa catcatcgac
     5401 atcaaatgaa cgggtagccc atccacgtgg gcggtatctt catcaaaagt cagaacatac
     5461 atcggccgtt tgaataaaaa gaacaatata ccgatggcaa cgaataagat tcctaaaaag
     5521 atgacttgct cccatgtaat ggtcacgatc gaaccaaaca gataggattg gatacttgtt
     5581 gctgaattcc ctccgctcaa attcatcaat actaacgcta gcgccaaacc accagccatt
     5641 aaaatggcaa tggagatttc tgaataggtt cggtagatcg ttcgcagata ctccaatatc
     5701 acagctgcta atacaacgac tagcatggtc gtaagtgtag gattaaggtt caaaaagaat
     5761 cctaacgcta cccctgctaa agagacatga gaaagcgtat cagccatcag cgattgtctt
     5821 ctgatcacta aaaacacacc tagcatcggg gcgattccgg caatgaagat agcggcaaga
     5881 aacgctcttc tcatgaattc atatgaaagc aacgccattg agaatcctcc tttctcacca
     5941 gtcggatctg tctgtccgca tactgtttga tatcttcatg atcatgggtg atcattagaa
     6001 ctgatttccc atggacatgc gcactatgtc ggagaagacg ataaaattca tttctggatt
     6061 cttcatccat acctgttgtt ggttcatcaa gaacaaataa gtcagggtca gtagcaaaaa
     6121 tacgcgctaa actgattcgc tgcttttgtc cgccagacaa ttcacctatt cgccgatttc
     6181 gcatatccca catccctaca gcttctaaag cacgctgtac atgttgatga tctttttcag
     6241 agagacgctt gaaccagcgg cctcttggat atcttcctga acggactaat tcgatcaccg
     6301 tactaggaaa acctgcattg aaagatgcaa cttgttgagg aatataaccg atacttaatt
     6361 tttttccttc ttgatttttc tcagcaatct ttatcattcc ttttgaaggt ttcagtaatc
     6421 ctaacgatgc tttcaccaag gttgacttgg ctgctccatt ttctccagtt aaaatgacaa
     6481 attcacccgc atctacatga taagagacat cttctagtac aggttcatca tcatagtaaa
     6541 aggtcaaatc agatatttcg atataacgca ccttacaacc tccattctaa aacttaattg
     6601 atactttctt ttaaagcttt cagatttttt tccatgacag aaatataatt ttcgcctgct
     6661 ttgatttgtt cattagtcaa gctttctaaa ggattcaaca cttctagctt cactcctgtt
     6721 tcattcgaca atgtctcagc taccttcgaa gaggcatttt cttcaaaata aatcactttg
     6781 atgttgtttt tcttcacaaa ttcttttagc tcagctaagc gacttggtgt gggttcctga
     6841 tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt attgattcgc taaataagca
     6901 aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat cttttagctc atcggagtat
     6961 ttttgatcca gtgcttcaag ctttttgata taagcatcgg cattcttcgt aaaagtttcc
     7021 tttttatctg gatatttttc gcctaattct tcagcaatcg ttctgacttc ttttatagct
     7081 aataccggat cgagccaaac atgtggatcg tattcatgtg aatgaccatg cccttcttct
     7141 gattcttcat gttcgtctgc ttcttgcgat tctaaacggt cgattccttt agctgcttcg
     7201 atcatcaagg tctgtttgct atccactgaa tcctttaacg aatccacaaa aaattccatg
     7261 tctgaactat tatacacgaa aacatctgca tcggaaattt ctgctctttc ttttgcactt
     7321 ggttcgaaat catgcggctc cgttccagca gggatcaaaa gtttcacgtt tccttcatcc
     7381 cctacgatct cttttgtaaa atcatacatc ggataaaaag tcgtaacgat cgtcaaatcc
     7441 tcttttttgt ctgcttcctt gttcgtattt ccacatgctg caaacagtaa agcacctatc
     7501 attaccgtag ctgtcaaaag atattttttc atttatatgc tcctttcaat gaacaaatcg
     7561 gaattttacg atttattaac gtgaactaat gtatcaaaat aaaaaaacac agtcaataga
     7621 aatccatcaa tttctgattt aagcatgtat attcttgatt aaatcgtaaa atggatgagt
     7681 tgctgctcat atctagccat ttcactacaa taaaaaactg aagaatacca ttttaggatt
     7741 cctcagtttt tcatcaccta ttttacttct tatattttat tgcagggttc gtactaaata
     7801 aacttcctca caaaaacctt ttaacccatt atatactctc tgcgctcttg aataatgttg
     7861 acacaaggca aaaacggttg gtccgcttcc actcatcaat gcagcatctg ctccgtactt
     7921 gatcatacgc tctttgattt gatggatgac tggatgccgc tttcccgtta tatcttctaa
     7981 actgtttccc atttttttga tcatctgctg ataatcttgc tctcggatgg cttcagcaag
     8041 tccgtggatg tcttgatgtt ggatggcttg cgtatctact tctctaaaca ctgtacgtgt
     8101 agagacactc attcttggtt tgaccaacac aacccagcac tgtggcatag aaggtaatac
     8161 ttggatttct tcccctttgc caccgacaaa tgccgtactt ccgtagacac agtaaggaac
     8221 atctgtaccg acttccaaac cgatttctgc cagttcttct aacgaacagc ccagattcca
     8281 taattggttg atccctctta gagcagctgc acagtctgta cttcctccgc ctaatccagc
     8341 tgctactgga atattcttct taacaaagat cttgacaccg gttttgatat tgtatttatt
     8401 ttttagcaat aaagcagctt gataaacatt gttccgttca tctaccggta aaaatgcttt
     8461 atttgtttct acaatgattt tgtcttctgt tatttcttca aaaaatagat gatctgccaa
     8521 gtctacgctc gacatcacca tttccaactc atgataccca tcttgccgtc tatataatac
     8581 atccagtcct aaatttatct tggctggcgc tttttcaatc aattccatcc tctcacccat
     8641 tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa ataaaagtat agcttatgtc
     8701 tgcctagtgg ttgattttgt catcgtttca acagctttca cagaattttc tcttcataat
     8761 ccaaacaagt tctagtgatt cttataattc tgtgagcact tcttctgcca aactcggaac
     8821 atttcctttt tcttttccta tcagactgag tagctgcatg gtagcaaatc cgaaattact
     8881 tcctttccta taagcaaaat aacgtccttc ccaatgatcg acatatttta atttcttttc
     8941 ataagcagac tggaaggaat agaaaaaatt accataatta tatacgatat gcatgacttt
     9001 ttcttgaaag aaagaaaaat ctgtttctcc aacattagct aatggagcta agcctagatt
     9061 ggcttccgag tagcccattt tctcgtatac aataaacaga ttggctaaca gatagtcagc
     9121 taattcctca gattctttag gtaagacccg caacaaatca tatcccgcct gcttcttcgt
     9181 catcggctga gcggtgatga agccaacgac tttttgatct ttacggacaa taccgacacc
     9241 gctagataat acatatgatt tatcaaatcg tcccacagag aagtattttt ctcgtgcacc
     9301 ttgaagccat ttttgtgaaa cttcatttac agcttgatgt aattcttccg gcagttgatc
     9361 gtaccaagta aaatggtatc cttctttcat caaacgttga tattcaagtt gctgactcag
     9421 ccgagacaag tcttctgttg ctagatcaac aatccctgct tcacctactt tcgtgaagtg
     9481 aaaacccaat tcatgaagca tcactacgta gctttctgat atcttataaa acgcaagctg
     9541 atagcctaaa caatctgctt gtttcataaa ctgattcgtt gctgcccgca tcattttagg
     9601 attgccaact ggatctccta aaacaaagca tttgtttcct ttgatctgat aaccaaaaac
     9661 gacttgatct tggtcgtctt cttggtaata aaaataactg taacctgaca agcgaagctg
     9721 atggcttgta gctgtgcctc cgtatgtttc caataaacgg tcaaaacggt ctttttcgta
     9781 ctcactgcca agcttcgtag tatcttcagc taaatattga taaagcgcca ctaaagctag
     9841 caatgagatg cccaaaccaa taagccctga gaaccacaca tcatctgaag gaaacaaaat
     9901 aaaatgattt cctgttattc gaccgactaa aaagcgtccg ctgtgatagc ctgcaattgc
     9961 gtaaacgata aacaaaaagc cgaacaagct agcatcaaag attatagctc cccaagaata
    10021 aacgaatttt gttcgataaa attcttttct ggataaccac acagctaaca gaatcaagac
    10081 atataccaat atcaactgcc aagataacgt acgtgtgatt gtatttaaaa taccaaaaga
    10141 tagtaaagca atcgtaggca gaaatgcctt tttgactttt actgaaagtg ctctagcaag
    10201 gccgagtaat aaaaagccaa ccaatagatt cactgtctga tctaaaaaat tgaatgagaa
    10261 cggcaataat acttgaaata gccgactgat tgcagacaga ttagtgattg ttgatagaag
    10321 taccatcatg atccccgcaa aatagacagc acttacgagc acgatatgcg caaccttttg
    10381 gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt ttgactcctg tatgtgtgat
    10441 gaacaataga agtcccgtca taaaaggtag aacataataa aacaaccgat aatataatag
    10501 ccaaactact gctacatctt gccgaacaga caattgactc attcctaaga tcatcaaaac
    10561 gtcaaaagtc cccattccac ctgggaccat cgtcagcatt cctatcaatg tagcaatcat
    10621 gaacatagga tagatcgaaa gcatcgaaat atctattctc atcaacttgc cgatgatcaa
    10681 aaataccgca agggctcccg tccattgtcc aaaggatgcc agtagcagac caagcaagcc
    10741 ttttggataa aactcgaaga acaatgtctt tctctttaga taagcaaaaa agaaaatgac
    10801 tggtgctaaa aaactaccgc cgaaaagcca gatccaatag cttcgaaaaa tactttctga
    10861 ctgaataaaa aagacatcaa taaatgtaag aaacgaccat acagacaaac cagtcagcat
    10921 aaatagtgcg acttttgata cagtggctaa aaccattttt ctatttgtct gctttccata
    10981 aaaactagcc cgcagacttg ctcctaccac accaccaaaa ccggcgagat tgttgatcgt
    11041 gtttgttacc cacgcagcaa gaagaaactc ttttctttcc atttttggtt ttccttgttt
    11101 ttctaagact ctgatcgtca cccaatcata caacaacatc ggagtgacac ccagcagtcc
    11161 agccagaatc atgagcagga tcgtcatgcg gctttgttgt cccatccttt gccaaatatc
    11221 ttgccaactc atgccattag caatatttgc gacttgattt gccacaaaga ctaacacgga
    11281 gccgaaaaaa atcagtttta aaatcagact atgttccttt atccattgca gcatcaagta
    11341 tcgtttattt ttcacatttt cccattccta acttgtatac ttttgtgtca taccccaatg
    11401 ttatcttatt tattttaatt tttccacttt agacctgttc tttgcttgcg atgatagaaa
    11461 aagaaagata tcctaatgct aaaggagagg ataacatgaa agtaggattt atcggcacag
    11521 ggaatatggc acaagctatt attgatggtt tattaaaaaa aggaacagtc tcgaaagaag
    11581 aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa atttgctgct gaaactggcg
    11641 tccatgcatg caaaagtaat caggaagttg catcacaatc agatatcgtt cttttagccg
    11701 tgaaaccaaa aatcattgaa aaagtattgg aagaactaag agaatatagc cagaaagtct
    11761 tattcatttc tattgcttca ggaaaatcat taatagattt agaaacagcg cttgaagatc
    11821 ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt cagcgtagga gcaggcgttt
    11881 ctgcgatttg caaaaatgaa gctgccacgg aggaacagct tcagcaagct acgatgattt
    11941 ttgaagcaat cggcagcgtc tatcctttag cagaatcaga tttcagtaca ttcattggcc
    12001 ttgctggcag ctcacccgca ttcgtttaca tgctgatcga tgcgatggga cggactggtg
    12061 tactgaatgg tttgtccaaa cagacagcca ctgagattgc tgcaaaagca attatgggtt
    12121 cttgtcaaaa attcctttca agtgataaaa atccatggga acttgtagac caagtttctt
    12181 ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga agaagcagga ttcatcccag
    12241 caatcatcaa aggaatcaat acgacgatcg agaaagatct tgagatgttg aataaaaaat
    12301 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
    12361 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
    12421 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
    12481 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
    12541 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
    12601 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
    12661 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
    12721 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
    12781 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
    12841 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
    12901 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
    12961 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
    13021 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
    13081 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
    13141 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
    13201 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
    13261 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
    13321 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
    13381 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
    13441 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
    13501 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
    13561 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
    13621 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
    13681 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
    13741 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
    13801 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
    13861 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
    13921 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
    13981 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
    14041 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
    14101 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
    14161 catataggta aacctttaaa ttgagaaact tttctattaa attattcttt gtagatgaac
    14221 agatattttt attttatgta cccactcgta taattaaata actcacgatt tctttattca
    14281 ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag accagccgtt tttgtttatc
    14341 ataattttta ttcttctact aaaggcaatt cttcttgtcc tttttcatct tctgggacag
    14401 cgacttcttc accaatacca taagctgctc taactagagc agaaacttct gccatcattt
    14461 caggatgatt agccatatag attttagcgt tttcccgtcc ttgaccaatc cgatcttctt
    14521 tataagaata ccaagcaccg cttttatcaa caatgtcttt ttctactgcc atgtctagaa
    14581 gttctccttc ttgtgagatc cctaaaccat acatgacatc tacttcagcg attttgaaag
    14641 gaggtgctac tttgtttttt acgactttga tctttgtacg gttaccaaca atatctgtac
    14701 cttgtttcaa ttgttctgca cgacgtactt ccaaacggat cgttgcgtag aatttcaatg
    14761 cgcgtcctcc aggagtgatt tctggattcg caaacatcac gcctactttt tcacggattt
    14821 ggttgatgaa aatagcgatt gtttttgttt tgttgatcga accagagagt ttacgcaatg
    14881 cttgagacat caaacgtgct tgtaacccga catgtgagtc acccatttcg ccgtcgattt
    14941 ctgctcgtgg aactaaagca gcaactgaat cgactactac gatatctaca gccccacttg
    15001 atactaaagc atcagcgatc tctagacctt gttctcctgt gtcaggctgt gaaagaagta
    15061 gttcatcgat attcacacct aatttttgtg catattgcgg atctaacgca tgctcagcat
    15121 caatgaaagc ggccgttccg ccattttttt gtacttctgc aatagcgtgt agtgcaactg
    15181 ttgttttacc tgaactttca ggtccatata cttcgatgat acgtccacgg gggtaaccgc
    15241 cgacacctaa agcgacatct aacgcaagag agccacttgg aattgtagag atttgttggt
    15301 cgattttttc gcccaatttc ataatagagc ctttaccata gctcttttca atttttttca
    15361 gtgcagcatc taaggctgct ttacgatcat ctgccaatac gataatcctc cttatattta
    15421 caaatcagag caacatttca ttatccttat catatctttt ttagtgaaaa aaagcaagaa
    15481 aaaaccgaac aaatattcgc atttatcatt ttcttaaaag ctgttttctc aaaagatcaa
    15541 atccttgcat aaccgcatta tatctgatag cagcacgatc tctggcaaag tgatattttt
    15601 ccgaaaccac gccgtcttta gatgaaatag cgatccatac tgttcctgca gattgacctt
    15661 ctaacgtgtc cggtccagcc actccggtga aagctacact gtaatctgtt cttgctatct
    15721 cacgtgcttg aatggccatc tgctccacac attctttgct gactgttcca tattctgcta
    15781 atagctccgg atcaatctcc agaaaaccag cttttgtttg gagcgagtag gtaacgaatc
    15841 cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg
    15901 ctgtcaaact ttccgctgct gttacagtct gcttgttttc tttcagtagt tccactacga
    15961 cttttgctag cgaattatca tcgccgtatc cataaaaata ttcccctact cgttcttgga
    16021 ttttttcttc taatgctaaa agagcttgat tccctgcctg aacatcattt gttttcactg
    16081 tcagtcgtaa tgtcacttca tttggtttgg catatggtgc gatcgttgga ttgatctgtg
    16141 tctcaatcag atcttttagc tcagtgacca gtcgcgattc accaatccca taaaagcgca
    16201 aaactttaga aattagtttt tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt
    16261 gttccacaaa cattggtttc aactcattag gcggaccggg aagaaggata tacgcatgtg
    16321 tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg atttggtaga ggaaccccgc
    16381 cttcgataat ctgggactgt tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa
    16441 aatatgccaa taattttttg tatccttccg tattttgtat gagagactta ccgaggtgag
    16501 ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc taatccgcca cataagagta
    16561 tgagttcact tcttgtttca gctagagcaa tcaactcttc taaacgctgc tgattatctc
    16621 ctacaactgt ttgataataa acttcgatac ctagatcagc tagctgttct gacaagaaag
    16681 tggcattcgt attcacgact tgtcctagta aaagttctgt tccgacagct attatttctg
    16741 ctttcattac ttcccgctcc cttatatagt atacgctgta tttttatatt tttttattta
    16801 aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc tccttgttcc aaataaacag
    16861 tgttctcctg ccaaaacctc ggtcataagt cacaatatac ataaaacatg aaaagctgtt
    16921 ttttgaaatt atcccttgtg actcagagtt agacggcagg ctcacaacct ctccactaaa
    16981 cgatacttac attgatcctt tgaacacacc gctgtttttg atgaagtaat ccacacctga
    17041 atagatagta aagatcagac aagcataaag catgatttga tctactggaa taccgatcag
    17101 gctgaatggg atattattga taaacagcaa gataatagca accatttgtg tagccgtctt
    17161 gacttttccc ggccaagctg cagccatgac ttctccatgt tcgacaagta gtaaacgcaa
    17221 gcctgtgacg gctaattcac gacagacaat gatcgctact acccaaccag gggcctttcc
    17281 ttggtcaacc aacatgataa aagcagtcat aactagcatt ttatctgcca acgggtccgc
    17341 aaacttgcca aaattagtga caaggcctcg cgcacgggca atttttccat ctaaccagtc
    17401 tgtgatacta gctaaagcaa aaataactgc acctactagc tgtgtgacag ccagtgtggt
    17461 gtctcctacg ttaaattgtc cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg
    17521 gatcatacaa attctaagta cagttaattt atttggtaaa ttcacactat cgactccttt
    17581 cttccctatt ctacgtgact atcagcgaag tttccaccga tattatttac ttttccaaat
    17641 gaatttattc aaaggagtat ttttattgaa attgatcttt ttgaattaac gaagaaggac
    17701 cgcgacacag atttgtcacg atccttcttt tttataccta caagtaaaca gatgcgtaaa
    17761 ctaatttata cgatcattct gcgtattgaa gagtaaggtt cacatttttt ggactctgat
    17821 tgttttcccc tggattaact ggtacggatt ctccattcgc tttgatcgtt gcattgcttg
    17881 ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg taacacggta ctctgtgttt
    17941 catttgctgc cagtgtccct tgataaacat aggcattgtc tactagcaca cctaaccata
    18001 cacgattgac agcagtaaat tcaagggtta caggcttggt agcgtctgtg atatttactg
    18061 cgatggcact gccagaatct tctcctgtag ctattgccat tttcttttct tcacttgatt
    18121 ttgttgttga ttctactgta gaagactctt tggtctgcgt gctttgctct gaggaagagg
    18181 actcctcgac tgatccttct actttaagcg aagaagctgt gtctccgatt attggatcag
    18241 cttgacgatc ttgccaagtc atatagccca cgacaacgac aatcgccaaa gcaaccaatc
    18301 ctaataaaat gacaggaaga cttgtccaaa agcgacttgg atgcgattct tcctgatgca
    18361 ttgcttttct agaagtactt actgtttcag gctgcggacg ttcagggacc gatcctgtca
    18421 atttgctttt cccatcaaaa acatcgacta aatggtctcc atcttctcct acagcttcag
    18481 cgtattgccg aataaatgcc cgcacgtaaa atttaccagg caattggtca aaatcatcat
    18541 tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag
    18601 atattttttt attcaggcgt gcttgccgta atttttctcc gatggtttca aaagccactc
    18661 gttcatcttc tccagtctgt ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt
    18721 atgtttgggt gctgtgacaa aagtcatgtc acagctcctt ttttccgaat aaacattgtt
    18781 ctcctgccaa ctcctcggtc acaagtcaca atatccaata aatataagaa gctgtttttt
    18841 gaacttgttc cttgtgactc ggagttaaac ggcaggctca cgacctcttt cttaaacggt
    18901 gttcaatcag ctgttcctcg gtattagctc aaaaaccggc aaaatataag gagatatttt
    18961 tccgtatgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
    19021 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct
    19081 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttggta
    19141 cacggtgttc cagaagtagc ttcttcggca ataagccgaa atatcacaaa aatttgagaa
    19201 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcttt
    19261 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
    19321 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
    19381 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
    19441 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
    19501 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
    19561 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
    19621 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
    19681 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
    19741 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
    19801 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
    19861 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
    19921 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
    19981 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
    20041 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
    20101 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
    20161 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
    20221 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
    20281 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
    20341 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
    20401 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
    20461 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
    20521 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
    20581 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
    20641 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
    20701 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
    20761 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
    20821 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
    20881 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
    20941 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
    21001 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
    21061 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
    21121 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
    21181 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
    21241 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
    21301 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
    21361 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
    21421 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
    21481 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
    21541 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
    21601 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
    21661 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
    21721 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
    21781 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
    21841 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
    21901 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
    21961 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
    22021 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
    22081 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
    22141 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
    22201 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
    22261 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
    22321 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
    22381 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
    22441 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
    22501 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
    22561 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
    22621 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
    22681 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
    22741 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
    22801 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
    22861 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
    22921 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
    22981 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
    23041 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
    23101 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
    23161 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
    23221 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
    23281 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
    23341 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
    23401 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
    23461 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
    23521 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
    23581 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
    23641 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
    23701 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
    23761 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
    23821 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
    23881 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
    23941 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
    24001 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
    24061 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
    24121 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
    24181 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
    24241 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
    24301 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
    24361 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
    24421 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
    24481 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
    24541 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
    24601 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
    24661 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
    24721 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
    24781 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
    24841 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
    24901 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
    24961 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
    25021 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
    25081 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
    25141 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
    25201 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
    25261 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
    25321 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
    25381 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
    25441 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
    25501 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
    25561 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
    25621 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
    25681 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
    25741 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
    25801 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
    25861 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
    25921 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
    25981 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
    26041 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
    26101 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
    26161 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
    26221 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
    26281 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
    26341 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
    26401 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
    26461 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
    26521 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
    26581 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
    26641 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
    26701 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
    26761 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
    26821 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
    26881 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
    26941 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
    27001 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
    27061 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
    27121 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
    27181 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
    27241 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
    27301 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
    27361 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
    27421 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
    27481 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
    27541 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
    27601 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
    27661 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
    27721 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
    27781 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
    27841 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
    27901 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
    27961 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
    28021 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
    28081 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
    28141 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
    28201 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
    28261 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
    28321 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
    28381 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
    28441 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
    28501 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
    28561 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
    28621 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
    28681 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
    28741 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
    28801 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
    28861 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
    28921 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
    28981 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
    29041 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
    29101 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
    29161 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
    29221 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    29281 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    29341 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    29401 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    29461 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    29521 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    29581 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    29641 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    29701 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    29761 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    29821 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    29881 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    29941 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    30001 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    30061 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    30121 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    30181 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    30241 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    30301 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    30361 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    30421 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    30481 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    30541 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    30601 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    30661 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    30721 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    30781 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    30841 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    30901 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    30961 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    31021 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    31081 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    31141 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    31201 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    31261 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    31321 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    31381 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    31441 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    31501 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    31561 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    31621 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    31681 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    31741 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    31801 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    31861 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    31921 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    31981 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    32041 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    32101 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    32161 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    32221 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    32281 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    32341 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    32401 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    32461 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    32521 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    32581 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    32641 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    32701 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    32761 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    32821 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    32881 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    32941 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    33001 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    33061 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    33121 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    33181 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    33241 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    33301 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    33361 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    33421 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    33481 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    33541 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    33601 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    33661 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    33721 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    33781 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    33841 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    33901 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    33961 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    34021 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    34081 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    34141 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    34201 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    34261 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    34321 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    34381 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    34441 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    34501 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    34561 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    34621 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    34681 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    34741 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    34801 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    34861 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    34921 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    34981 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    35041 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    35101 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    35161 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    35221 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    35281 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    35341 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    35401 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    35461 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    35521 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    35581 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    35641 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    35701 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    35761 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    35821 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    35881 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    35941 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    36001 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    36061 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    36121 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    36181 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    36241 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    36301 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    36361 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    36421 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    36481 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    36541 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    36601 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    36661 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    36721 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    36781 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    36841 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    36901 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    36961 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    37021 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    37081 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    37141 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    37201 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    37261 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    37321 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    37381 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    37441 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    37501 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    37561 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    37621 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    37681 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    37741 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    37801 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    37861 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    37921 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    37981 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    38041 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    38101 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    38161 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    38221 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    38281 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    38341 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    38401 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    38461 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    38521 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    38581 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    38641 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    38701 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    38761 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    38821 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    38881 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    38941 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    39001 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    39061 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    39121 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    39181 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    39241 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    39301 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    39361 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    39421 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    39481 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    39541 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    39601 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    39661 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    39721 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    39781 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    39841 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    39901 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    39961 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    40021 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    40081 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    40141 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    40201 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    40261 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    40321 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    40381 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    40441 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    40501 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    40561 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    40621 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    40681 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    40741 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    40801 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    40861 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    40921 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    40981 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    41041 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    41101 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    41161 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    41221 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    41281 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    41341 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    41401 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    41461 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    41521 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    41581 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    41641 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    41701 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    41761 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    41821 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    41881 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    41941 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    42001 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    42061 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    42121 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    42181 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    42241 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    42301 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    42361 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    42421 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    42481 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    42541 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    42601 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    42661 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    42721 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    42781 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    42841 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    42901 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    42961 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    43021 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    43081 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    43141 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    43201 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    43261 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    43321 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    43381 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    43441 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    43501 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    43561 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    43621 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    43681 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    43741 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    43801 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    43861 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    43921 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    43981 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    44041 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    44101 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    44161 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    44221 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    44281 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    44341 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    44401 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    44461 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    44521 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    44581 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    44641 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    44701 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    44761 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    44821 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    44881 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    44941 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    45001 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    45061 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    45121 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    45181 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    45241 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    45301 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    45361 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    45421 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    45481 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    45541 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    45601 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    45661 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    45721 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    45781 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    45841 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    45901 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    45961 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    46021 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    46081 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    46141 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    46201 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    46261 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    46321 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    46381 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    46441 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    46501 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    46561 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    46621 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    46681 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    46741 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
    46801 aattccagta aattactata attataaaaa aagaattgac aaaacataac tgtctatata
    46861 aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa
    46921 tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat
    46981 caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa
    47041 aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat
    47101 tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga
    47161 acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat
    47221 tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat
    47281 ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga
    47341 gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta
    47401 cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat
    47461 tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg
    47521 tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt
    47581 agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag
    47641 actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt
    47701 agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa
    47761 aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg
    47821 ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa
    47881 tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt
    47941 ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct
    48001 tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa
    48061 ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct
    48121 gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca
    48181 gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt
    48241 aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac
    48301 gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg
    48361 gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac
    48421 tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt
    48481 tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg
    48541 ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca
    48601 aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca
    48661 gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc
    48721 ttgatgttag cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc
    48781 tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct
    48841 ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc
    48901 gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa
    48961 ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat
    49021 ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat
    49081 tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac
    49141 aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc
    49201 ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga
    49261 aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa
    49321 ctacatggtg gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca
    49381 tttgccggtg gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca
    49441 atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta
    49501 ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca
    49561 acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc
    49621 atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa
    49681 gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa
    49741 aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa
    49801 cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc
    49861 atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc
    49921 tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca
    49981 gccaaacgta acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga
    50041 acagcttttt tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc
    50101 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta
    50161 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    50221 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    50281 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    50341 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    50401 ttttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    50461 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    50521 attcacgttt caatacccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    50581 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    50641 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    50701 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    50761 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    50821 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    50881 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    50941 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    51001 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    51061 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    51121 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    51181 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    51241 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    51301 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    51361 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    51421 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    51481 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    51541 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    51601 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    51661 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    51721 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    51781 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    51841 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    51901 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    51961 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    52021 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa
    52081 caagatacgt tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg
    52141 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    52201 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    52261 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    52321 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    52381 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    52441 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    52501 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    52561 cttcgactaa ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    52621 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    52681 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    52741 gcatcaaact gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta
    52801 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    52861 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    52921 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    52981 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga
    53041 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    53101 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag
    53161 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    53221 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    53281 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    53341 ttatcttcct ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    53401 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    53461 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    53521 ataaattttg gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc
    53581 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    53641 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    53701 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    53761 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    53821 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttt
//
LOCUS       DANKAY010000007        53595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000007 DANKAY010000000
VERSION     DANKAY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1350
                     /locus_tag="PWY98_000415"
     CDS             <1..1350
                     /locus_tag="PWY98_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2036200.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="PWY98_000416"
     CDS             1484..2827
                     /locus_tag="PWY98_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2036201.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="PWY98_000417"
     CDS             2939..4390
                     /locus_tag="PWY98_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2036202.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="PWY98_000418"
     CDS             4739..5371
                     /locus_tag="PWY98_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2036203.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5959..7398
                     /locus_tag="PWY98_000419"
     CDS             5959..7398
                     /locus_tag="PWY98_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2036204.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7693..7911
                     /locus_tag="PWY98_000420"
                     /pseudo
     CDS             7693..7911
                     /locus_tag="PWY98_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8160..8375
                     /locus_tag="PWY98_000421"
     CDS             8160..8375
                     /locus_tag="PWY98_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036205.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8592..8848)
                     /locus_tag="PWY98_000422"
                     /pseudo
     CDS             complement(8592..8848)
                     /locus_tag="PWY98_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9035..9946)
                     /locus_tag="PWY98_000423"
     CDS             complement(9035..9946)
                     /locus_tag="PWY98_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2036206.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9965..10969)
                     /locus_tag="PWY98_000424"
     CDS             complement(9965..10969)
                     /locus_tag="PWY98_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2036207.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10966..13089)
                     /locus_tag="PWY98_000425"
     CDS             complement(10966..13089)
                     /locus_tag="PWY98_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2036208.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13094..15541)
                     /locus_tag="PWY98_000426"
     CDS             complement(13094..15541)
                     /locus_tag="PWY98_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2036209.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15528..15917)
                     /locus_tag="PWY98_000427"
     CDS             complement(15528..15917)
                     /locus_tag="PWY98_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2036210.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15977..16480)
                     /locus_tag="PWY98_000428"
     CDS             complement(15977..16480)
                     /locus_tag="PWY98_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2036211.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16493..16717)
                     /locus_tag="PWY98_000429"
     CDS             complement(16493..16717)
                     /locus_tag="PWY98_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036212.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16821..18731)
                     /gene="ltrA"
                     /locus_tag="PWY98_000430"
     CDS             complement(16821..18731)
                     /gene="ltrA"
                     /locus_tag="PWY98_000430"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2036213.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19477..19614)
                     /locus_tag="PWY98_000431"
     CDS             complement(19477..19614)
                     /locus_tag="PWY98_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2036214.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(19611..20795)
                     /locus_tag="PWY98_000432"
     CDS             complement(19611..20795)
                     /locus_tag="PWY98_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2036215.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(21054..21344)
                     /locus_tag="PWY98_000433"
     CDS             complement(21054..21344)
                     /locus_tag="PWY98_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036216.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(21334..22476)
                     /gene="dcm"
                     /locus_tag="PWY98_000434"
                     /pseudo
     CDS             complement(21334..22476)
                     /gene="dcm"
                     /locus_tag="PWY98_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22552..22716)
                     /locus_tag="PWY98_000435"
     CDS             complement(22552..22716)
                     /locus_tag="PWY98_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036217.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(22709..23383)
                     /locus_tag="PWY98_000436"
     CDS             complement(22709..23383)
                     /locus_tag="PWY98_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2036218.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(23461..24801)
                     /locus_tag="PWY98_000437"
     CDS             complement(23461..24801)
                     /locus_tag="PWY98_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2036219.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(24884..25219)
                     /locus_tag="PWY98_000438"
     CDS             complement(24884..25219)
                     /locus_tag="PWY98_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2036220.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(25229..25603)
                     /locus_tag="PWY98_000439"
     CDS             complement(25229..25603)
                     /locus_tag="PWY98_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2036221.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(25616..25930)
                     /locus_tag="PWY98_000440"
     CDS             complement(25616..25930)
                     /locus_tag="PWY98_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2036222.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(26043..29270)
                     /gene="fss3"
                     /locus_tag="PWY98_000441"
     CDS             complement(26043..29270)
                     /gene="fss3"
                     /locus_tag="PWY98_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2036223.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            30058..30381
                     /locus_tag="PWY98_000442"
     CDS             30058..30381
                     /locus_tag="PWY98_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036224.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            30545..31462
                     /locus_tag="PWY98_000443"
     CDS             30545..31462
                     /locus_tag="PWY98_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2036225.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            31455..32237
                     /locus_tag="PWY98_000444"
     CDS             31455..32237
                     /locus_tag="PWY98_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036226.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            32238..32918
                     /locus_tag="PWY98_000445"
     CDS             32238..32918
                     /locus_tag="PWY98_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2036227.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            32911..33807
                     /locus_tag="PWY98_000446"
     CDS             32911..33807
                     /locus_tag="PWY98_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2036228.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            34019..35941
                     /locus_tag="PWY98_000447"
     CDS             34019..35941
                     /locus_tag="PWY98_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2036229.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            35942..36388
                     /locus_tag="PWY98_000448"
     CDS             35942..36388
                     /locus_tag="PWY98_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2036230.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            36404..36718
                     /locus_tag="PWY98_000449"
     CDS             36404..36718
                     /locus_tag="PWY98_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL2036231.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            36744..37793
                     /locus_tag="PWY98_000450"
     CDS             36744..37793
                     /locus_tag="PWY98_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL2036232.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            37805..40408
                     /locus_tag="PWY98_000451"
     CDS             37805..40408
                     /locus_tag="PWY98_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2036233.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            40525..42174
                     /locus_tag="PWY98_000452"
     CDS             40525..42174
                     /locus_tag="PWY98_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2036234.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            42490..43425
                     /locus_tag="PWY98_000453"
     CDS             42490..43425
                     /locus_tag="PWY98_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036235.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            43418..44188
                     /locus_tag="PWY98_000454"
     CDS             43418..44188
                     /locus_tag="PWY98_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036236.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            44603..45586
                     /locus_tag="PWY98_000455"
     CDS             44603..45586
                     /locus_tag="PWY98_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2036237.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            45907..46944
                     /locus_tag="PWY98_000456"
     CDS             45907..46944
                     /locus_tag="PWY98_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2036238.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(47033..47356)
                     /locus_tag="PWY98_000457"
     CDS             complement(47033..47356)
                     /locus_tag="PWY98_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2036239.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(47343..48050)
                     /locus_tag="PWY98_000458"
     CDS             complement(47343..48050)
                     /locus_tag="PWY98_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2036240.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            complement(48349..49773)
                     /locus_tag="PWY98_000459"
     CDS             complement(48349..49773)
                     /locus_tag="PWY98_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2036241.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            49948..50661
                     /locus_tag="PWY98_000460"
     CDS             49948..50661
                     /locus_tag="PWY98_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2036242.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            50810..52141
                     /locus_tag="PWY98_000461"
     CDS             50810..52141
                     /locus_tag="PWY98_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2036243.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            52409..52561
                     /locus_tag="PWY98_000462"
                     /pseudo
     CDS             52409..52561
                     /locus_tag="PWY98_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(52706..53581)
                     /locus_tag="PWY98_000463"
     CDS             complement(52706..53581)
                     /locus_tag="PWY98_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036244.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
BASE COUNT        16785 a         9911 c        10087 g        16812 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     5641 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     5701 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     5761 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     5821 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     5881 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     5941 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     6001 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     6061 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     6121 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     6181 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     6241 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     6301 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     6361 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     6421 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     6481 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     6541 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     6601 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     6661 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     6721 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     6781 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     6841 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     6901 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     6961 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     7021 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     7081 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     7141 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     7201 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     7261 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     7321 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     7381 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     7441 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     7501 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     7561 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     7621 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     7681 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     7741 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     7801 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     7861 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     7921 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     7981 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     8041 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     8101 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     8161 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     8221 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     8281 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     8341 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     8401 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     8461 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     8521 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     8581 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     8641 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     8701 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     8761 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     8821 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     8881 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     8941 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     9001 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     9061 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     9121 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     9181 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     9241 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     9301 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     9361 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     9421 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     9481 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     9541 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     9601 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     9661 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     9721 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     9781 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     9841 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     9901 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     9961 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
    10021 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
    10081 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
    10141 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
    10201 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
    10261 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
    10321 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
    10381 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
    10441 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
    10501 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
    10561 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
    10621 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
    10681 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
    10741 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
    10801 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
    10861 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
    10921 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
    10981 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
    11041 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
    11101 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
    11161 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
    11221 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
    11281 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
    11341 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
    11401 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
    11461 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
    11521 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
    11581 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
    11641 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
    11701 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
    11761 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
    11821 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
    11881 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
    11941 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
    12001 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
    12061 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
    12121 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
    12181 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
    12241 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
    12301 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
    12361 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
    12421 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
    12481 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
    12541 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
    12601 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
    12661 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
    12721 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
    12781 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
    12841 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
    12901 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    12961 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    13021 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    13081 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    13141 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    13201 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    13261 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    13321 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    13381 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    13441 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    13501 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    13561 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    13621 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    13681 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    13741 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    13801 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    13861 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    13921 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    13981 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    14041 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    14101 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    14161 taactgaatc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    14221 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    14281 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    14341 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    14401 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    14461 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    14521 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    14581 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    14641 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    14701 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    14761 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    14821 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    14881 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    14941 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    15001 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    15061 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    15121 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    15181 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    15241 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    15301 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    15361 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    15421 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    15481 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    15541 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    15601 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    15661 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    15721 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    15781 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    15841 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    15901 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    15961 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    16021 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    16081 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    16141 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    16201 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    16261 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    16321 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaaccca attcgttctt
    16381 tcacttcatc aaaatcgaca ggtggggtaa accacgcccc caccaattca ccttcgttat
    16441 atttcccgag gttcgcaata taaacacgca tctgttccat cgtgcttccc ccttacgcac
    16501 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    16561 atccgaccgc aatcaaagca atcaccaaaa agccaatcag tttggaaaac tcccgcttaa
    16621 atcctaaaaa caaaccaatg accacaattg ccagtaagac taaggattgg gcattggtta
    16681 aaaaccattg atatatggat aggtaagttc ctgatattat aaatatctca aacttttccc
    16741 cccattcaca cggagcgtgc gactttcacc gcactccgcg ttccatcgtg tttatattga
    16801 aaagtattta tgtagtagac ctatatcaag tttaattctt gattgccagc taatactcta
    16861 tatttattta gtcttatgag ctggacttta tttagcttca atagagctat atatttttgt
    16921 attgtttctt ctgttttagc gtgtattaga cggtgtaccg cctcttttac caaaataaga
    16981 tttttataat cgtcatttcc accagctcca gttggtacaa tatgatgaca atgtatttca
    17041 ttagcttgtt gaaattcttc cagagttata gcacattttc catgttgagc agaaaatagc
    17101 gaaattcgat tgtctgcgta ttctattgtg cgattttcca aagataattt cattaactgg
    17161 tacatcagta atttatcaac acttagattg gtgtggataa attttcttcc ctctagagtg
    17221 tagcaattcg ctaaatacac ctttgccatt ggttttctgt gttggatata tccaacaggg
    17281 aaaataggtt cgccctcgcc agcaacatac cgaagcattt tagatttacc atatcttgtt
    17341 gcctcctttg tcgtaagttg tctgcctgtt tttctcaatc tgcccctttt gtttgtacct
    17401 aatctgttag tgaagatagc cattacagct ctattcagtt tcatacaatc cagatttaca
    17461 ttcgttgcaa tctgataata attttgcata cctaatacca ttgaattata aagtcttgtt
    17521 tcttctaatt ccgttttccc ttttgaggga gaagcaattt tctttgcctg tgataccagc
    17581 ttgtcttttt ggtttttgaa ttgtttatca cttatatgtg atttaacaac taacttcttt
    17641 cttctcggta tcattttaat cttgaaacca agaaagtcgg agtatctttt tctaacattt
    17701 acaattcttg ttttttcttg tgatacttcc aattttaatc gttgttcaat ccattgagtg
    17761 attgctattt tggctttttc agcactttcc ttgtagcggc agaaaactct aaaatcatct
    17821 gcataacgaa ccatatacat ttcctttaac tttgttttct tcatttcttt gtaagcgttg
    17881 cttttacatg ggctaccgct tttattgata tgaaccacat agttttttgt aacaggattt
    17941 tcctgccatt ggctttctac ccaatggtct aactcattaa gaacaatgtt tgctagtagt
    18001 ggggatatta ttcccccttg cggagttcct ttctctggat atgttattgt accgttagtc
    18061 attttgattg gagcttttaa tatccttttt aaaatgaata tcaagtgctt gtcccgtatt
    18121 cccatagccc atatctgttt tatcagcttg gaatggttca cgttgtcaaa gaaaccctta
    18181 atatcaaatt caatcacata atgtagctgg ctgatttgta ggcggttata ggttgctttt
    18241 attgcatttt caactgacct attaggtcta aaaccatagc tgttttcact aaatttagct
    18301 tcgcagattg gttctaatac ttgttttata cattgttgta taagcctgtc ccacatacaa
    18361 ggaataccta gaggtctggt tttaccattt ggttttggta tttcttttcg cctcactggc
    18421 ttaggtcgat acccatgtgg actgcctgca acaatgtatc ttactttttt gacaactgtt
    18481 tctgctggca atttgccaat atctttaatt attgtaccgt ctgttcctgc tgttttactg
    18541 cctgcatttg ctttgatatt tcgatatgcc agtaatatat tttctctcga taggataata
    18601 tccataagat gagtgaaaat atctccattc ttacttttgg catataaatc atcaaaggtg
    18661 ttttgcattt cgtaatattc agcgtgtctt aaatcttcta cacacaatgt ttcattttta
    18721 tttttaggca taaggcttca ctcctttact tgaaattgag tgagcttttt tatcttactc
    18781 gtaatcctta ttgaaacaac ataatacttt tctaatatat acacgacttg agggcattcc
    18841 tccattctca ttacaagaat ttcttaggtt cgccctctgc ttttcagcat tagttcaact
    18901 gcttattgtt cttcgtcagt ttattctctt gatgagaacc caataccttt cctcgttccg
    18961 ataatcttat ctatacatat atatccttag gtcactagct ataagcctgc tggcttgata
    19021 ccgcctgtaa cgttatatgg catttcataa cctatattct tactttgcca caccagctac
    19081 gatttctcgt acctttgcat ttctacaaag ttcaaattta gacccgtaca ttcgctgttt
    19141 cgtcagttcc ttatagaaca ttctaaccat agatatttta tagacccccg acctgttgga
    19201 tacgctaccc acctctggat agttttcgat agaagatata tgttcatcaa ctattacggt
    19261 atctttcagc cgtctttacc gagcttcaaa cattctgagt ttcctcatac aatgcttgtc
    19321 ggaggctcag ggcaggcgtt tcagagcgtt acttcatcat tccacctgtc agattatcag
    19381 ttctcctaat aaccaaactt tttcgttctt ttattagtgc cttagctttt cactaaggaa
    19441 cgagtcgcac ataaattttg tccgaaattc atcttgtcac tttcctttct tttccattgc
    19501 tcgatccgct gctgcacttg cattcatcaa gcacatcaca gtaacaccaa gcatactgcc
    19561 accaaatacc aaaaaaatat cttttacaat tcccatgacg attcctcttt tcatataatc
    19621 aaattttcaa ttgccatact ttgttgctcc aaaatctttt tatgtcggtc tgataagcgt
    19681 gcattctgta ccatatcctg gatataggtt gtccgattca atcgatccaa ctccttagcc
    19741 attttccacg taggggcgac ttgtcggcct agccaattca aggttcgttc aatggtatac
    19801 ggttttggct cggaagttaa ttgaatctct ttccgattcc gtccaataaa acgcccccat
    19861 tgttcattca agggccagtt ggttcgcctt ttaccttcta ctttatccgc aaatcgcaag
    19921 taacgattga taatggaaaa cgtggtgctc tcaatgctac gaccttttaa caaatcttga
    19981 atcgcatgaa aagcacggtc atttttcagt cgaatctcaa aacgattttt cgtctcagtt
    20041 tggtctaatg gaatccctaa cttgatatat tgttcatagt ctttttcata caaacagaaa
    20101 taaacctcac tcttgagact gccgatatat aaggtattgc ccatgccgtc cttttcatca
    20161 gcctttaaaa gttccccgga acgataactt ttaaaggtac gaaacaagga gatacattct
    20221 tccttctgac attttttcgt tagctcaggt atatccaata gccccactcg atcattgatt
    20281 gccaagtcca agcgtttcat cacgccaccg gcctttagac aatcctcgaa aaagtcgtac
    20341 cagctacgct tttgagcgag taaaaaagtt tcaaattgtc ggcaacctcg cccctttaat
    20401 tctaaaagca cacctttatc ttcttcatga gacaacatca ccacaatatc acccatgaca
    20461 tactgttctt gataggagta aaaggcataa tcttcatgga gcatatactc aaatttcaaa
    20521 tggagaattt cttgaataat ttttcgtgca tccgttgtgg gaaagcgaat ccttacatag
    20581 tcaaacagta acgtcaaagg caaatcggga ttaaagcttt ccagttggtc aaaaattttc
    20641 ctcatggttt ttatagatgc ggttacgcca ccattttcta tctgatttaa gtacggccga
    20701 ctaagtccac aagccgtagc caatttattt tgtgaaacgc cgtattcgtt gcgtcggtct
    20761 tttaatagcc gataatctaa attccgttgt gccaagttct cacctccaaa ttcaaggtgt
    20821 taactttttc aaatgagtta acacctatac aaattactta ctatctttga tacaacaagc
    20881 attcttaatg gttgcttact tattttttgt gtttttgtac ccccctgtta gatacggggg
    20941 tgagacatca tggtcggcta acgccgtcca tgaacaggca cagcaccacg gaggatttct
    21001 gcttcgcgaa tcctccgcgg tcagcgccta acagtcagtt gtgttggttt aggttactca
    21061 tctgtaatac catttacttc caactctgct ttcatttcta acaagtattg acgaatatct
    21121 ggttcttttg cttggtccgc aatccgctca aattctgcaa ttacttgatt tatttcaatt
    21181 cgagcatccg tctcctcatt cattaaatat ggatcgccag cgactttgac accattcttg
    21241 cgaatatcaa attttaaccg tcgcaattgt tcttctaatt ctgggtctga ctgaatcttt
    21301 ccaatcaccc aataaatttc tccaatcaac tcatcataaa ccatgtacat ccaactcttt
    21361 taatcttctg gcaatatcaa aaatgaccgg aactgttaca gagtttccgg cttgcttata
    21421 caactgacta tctgaattga cttgagaatc tcgatcaaaa gcccaatcgg gaaagccctg
    21481 aagtcgccaa cactctctag gtgttagctt ccgaatttga tactgatccg tgacgatcgc
    21541 ttgttggtct cctgttagta aggtatgaac actctgtttt cccagacgac ctcggcgact
    21601 tgtggaatta ggaaaagcca gattcacact gtcttctggc ttcacctctt gatattctgt
    21661 ttcatttttt cgaatcatga tttttgtacc accttgtttc gttgacaagg tgggggcggt
    21721 tccagtaact tcataaactc gattaccttg ttcaaacttt cccggtaata gtcctgctac
    21781 agagattcca tcccttgctg gcagtgcgat tttgattcct tcatcacctg ttaaggttgg
    21841 cgctacgcca tctgaaccat agacttgtcc acccattcct cgattggaag gattgatatt
    21901 taccaaattt ctgatgtttt gatcagatgt tgaagacggt ccaatgatag gaaatacttg
    21961 ttcggtacat gcgtctcgag aatgtccgat aaggaatatc cgctcccggc tttggggaat
    22021 gtaggccttt gagttaagca cctaccactc cacgtcatac cccaatccat ccagcgttcg
    22081 gaggatcgtc tcgaacgtag cccctccttc gtgattgagc aatcctcgga cgttctcaag
    22141 gaataaaagc ttaggtcgga gaagagtggt gaaccgagcg atttcaaaga ataaagtacc
    22201 tcgagtatcg gaaaatcttt gccgctttcc cgcaattgaa aaagcttggc acggaaatcc
    22261 gccgcaaagg atgtccactg gtccgaggga ttgaataaac tcatctgata cacttgtgat
    22321 gtcatgcatt tccacctcct tacttgtatc atgaattgct ttataactac gtctagcaaa
    22381 tttatcaatt tcacaaaaac cgatacaatg atggctcgcc tgttccatac ctaaacgaaa
    22441 gccaccaata ccggcgaata aatctaaaaa tctcatacat tacctcattc attgcaaaga
    22501 aaaaagccaa gaacattttg ctcttggctc atacattatt aatttgtcct actatttttt
    22561 atttaatcca actaatttcc gttgtttttc attggcctga tcggctacat atttaatcac
    22621 cgttttgaac tcttttgcag tagcattttc cgataaatcc gcaatgctct tattgtattt
    22681 ctttacaatt ttcgttactt gttctttctc agtcatgtaa ttgaacctcc aaatctttcc
    22741 tactataccc tagtggtaaa aataattcta gattatccgt agcataatga acttcggaaa
    22801 tattattcgt ataatctttt attatgatat gaagcaatac atctggatgt ttactcttta
    22861 cttttgcaat gttctctctt gatttgaaaa aagcgttaat aaatgccttt ttcggcacat
    22921 gacgtccctc tgcaatttca cgttcttgtg taaatttcca agcgatttta gggtcttgat
    22981 aaacatagaa aatcgtcact cgaaaatgtt ttttcaacgc ccgcgagata tttttttcag
    23041 cacttaatat tgcaaaagtt gcatccagaa taaaagaata gccatgatct aagacatatc
    23101 taaatgcctg ctctaccaac caagaagatg ctttttgaaa gagactagag ttttttccat
    23161 tataatcagg aaactgggtg cgaaagtaat ccgcatcaat aatcacatgg ttatcataat
    23221 tctcagctaa tccaatggca acctctgatt ttccagcccc aggactccct gccataaaaa
    23281 ctgcgtctcg tccaacttcg ggggatttcc ctttgataat ataagaaata aacttatcct
    23341 tatttgcttt tgcatactct aaataagaat cattactttc catattaatt atcccctttc
    23401 ttcctactat ataaaagtat tatacgttac ttgaagtata aagtcagtat tgaagatcac
    23461 tcaatcattc atcttttcta atttactaat tgaatctaag aaatcatacc cttttggtac
    23521 aagtggcgta taaaactcac taatcacact gccacctgta tccacataac ctcgaccttt
    23581 gatgtgcttc ataaagaagt ttttttcgac ctccccaaac atcatggaat atccgagttc
    23641 actcatacga cccaaagcca cgcgaaagtt gaattgatcg cggattccgt ctcctaaata
    23701 tttcgcatcc ggcctttggc aagccaaaat cagaaagaaa ccagattgac gacccaacat
    23761 cacgatttgt ttgagcttat tcaaaatcac cgcactttct ttcgtcgtta acatctccat
    23821 ataagccacg tattcatcaa agattaaaaa gttgggtgga agtccaagat aggcataatt
    23881 ctctcctgtt ttgtagttag acatttcttt cattgccttg ctacgagcca tcatacgttc
    23941 atagaaatct tccacacaag cagaaatttc ttccttttgt gaataaacat gaggcatcac
    24001 cgtacctaaa tcagctaaat ctgcattttt gggatcgagg ataaacaatt ctgcatctga
    24061 cttcaatagt gcttcgataa tggtgaggag gaaataggtc ttccctccac ccgtgccacc
    24121 agcgattaac atatggggta aagaatcata agcccatact tgatttttca tcaatcgcag
    24181 agtcccattt tctgccacta cttcgtctat cccaattcga ttggcgatca tatcatacag
    24241 taaagtatac tccacatagg agtctttgag ttccttttct actaactcac aatacagtcc
    24301 gctttctaac ttcttttcca attttaaaag ctgatcttgg tatttcccaa gtgaaatttg
    24361 aacgcgaata gacagtaatc catctttcat tcgataacag attttgggga agtaactgat
    24421 agtctctctg gttcggctac tattcaaatc tttgaaaaag ccttcacttt gagtttgttt
    24481 cacttcatac caatggttct ctagaatcat ccgagctagc ttttgtcgat gccacaagct
    24541 tcgccaatga tcaagaaaaa agtgaatata aagcaatatc atagctcctg aaaaagcgag
    24601 acttccaagc agacatacta aatcttttat agaccaaacg atttcaatag tagaaagtga
    24661 tagcttagaa atatcaaatt gtctaaactc ttgaaaataa aagacgctca tccaaccgat
    24721 gaacagagcg agtagccagc caagaacaaa gtgataaacc aagtgctgat ctcctgcgcg
    24781 tatgcgatgt cctttgtaca ttttgatggt ttccacctcc aatcgaaaaa gcccaatagg
    24841 atttcattag gcaatttcaa aaagttatgc tagtcattta ctgctacgag gaggggagtt
    24901 gtttcgtttc aaccaattga tttaaaaagc cttcaatagc atccggcatc tccacaccaa
    24961 tttcttccaa aatggattct gcctgccgac ataagttttc atttacttcg acaactataa
    25021 agaaagactc ttcttctcct aaccagtcta gtacatcaga gtcttcttca ctgtcgctat
    25081 tttctccaag taaaccttga tgtataatca gaaacatttt ttcatcagag gttaatgctt
    25141 taacgagttg ttcctcagtc agctcgcctt ttccatattg atcgagacaa tctaaaatat
    25201 gttcaacatt aatttccata atcttcacct atttctttct ttcattttgg ttcgtaggat
    25261 ttccttgatt cggctgccgc ttcaagacta aatcatccgc cttaatgaac caattaacgg
    25321 tagttccttc tcgaaaaaca taattggcaa cggtatccac gattggattg accaactcta
    25381 cctctgcatc ataagcaaac tcgcgtaagg gtacattagc tgggatacta acttgaatca
    25441 ttcgcccttg tgcagaggat tttaaattat aggttcgttc ttttacctca ttaccaattg
    25501 ttccatcctc attttgcttc cgtacttctc ggcgtaatgc ggaaaaacgc aacttcccaa
    25561 atgttaaacc tgaatctacg acaatacctt ctgttaatct catcttcttt tcctcctatg
    25621 ctttcactaa atcatcaaca tacaacacgt aatcagtaaa cccacgattc tcaatctcat
    25681 agcctttggc aaccaaatgg ggattcacgg cttttagcgg ttggtcttga tcaaaatcct
    25741 tttcaccggc ttccgcagct actaccacct caatatcatc ggcccgttgc ttactggaat
    25801 acaaatgata actgcgacca atcaccgtag tattccgtcc atatccttct gttataattt
    25861 ctccttcacc ggcataactt aactttccaa aggtttcagc catatctggc acgacaaatt
    25921 tcaattccat cttcttttcc tcttttcatt gttagttttg gatacaaaaa tagcgcgact
    25981 acttttttag gaggcggtcg cgcacgttgg ttcttaggat ttacttgtat catctcatca
    26041 ccttagttcc ggcgattcca gaaataatag cccgccgtcg caaaaatcaa agtaaaacca
    26101 atgaacagta aaacattgga atttttctcc ccggtttgag ggaaggtctt cactgtggga
    26161 ctagattctt gagagccact tggaacagtt ggtgtttccg gttgtttcgg cgtacttagt
    26221 atggttggaa cttcttttgg tgttaaggtt tgagattttt ctttcaaatc ttcattgtgt
    26281 tttccattga tattcccatc tttatcgtaa ttgatttccg caaaagtaaa cttggttcct
    26341 tctggatatt ttccggtatc tacttttttc gcaagcacag ttttggtgaa ttctttatca
    26401 tttacttcat aatcaatctt gccagatttc caaatttctt tgtttgtacc atccggtaat
    26461 aaagcataca gaattgtttc gaaagcttct tttgagccat ccagtacatc atgagtaatc
    26521 gacacatcat caaacatatc caccacatca ccatgagtaa aggtctgaga accgttttct
    26581 aggtgggcct ttgtttggat agaaacatgg tgttccactg tacaactgac agtttgggct
    26641 tggttattca agctaacatc cttcgctact ggcttatcgc ctgcttcaaa agcttcctta
    26701 ttttcataca catagttgaa taagacaata gttttattta aaacttgttc ggcggttaat
    26761 ttatgctgaa ttttccaagt tcctgttttc tctttgttcg ccaacgttgc gcttgtttct
    26821 gcaatcacca ctggtttggc tttttcatct ttttcttggg cagcttttgt cgcatccaca
    26881 tcaatggctt gcgctaccac ataccagtct tctttgattt catgtagatt gtaactcaat
    26941 ttatcaacta gctcggtaga atccagagaa gttaattctt tgtttccgtc tttccaagtc
    27001 gcaagagaag ttaaactatc ttcttcctca ggcaaatcat agagcattaa acggttcaaa
    27061 ctaacagaaa atgctttgtt cgtcagcttc tcgtaaggaa cggtcaccgt tttaattggt
    27121 tgtttttggt cttgttcggt aatggtaaag acatactcac tcttcacaaa gtcttctttg
    27181 ttttccttaa aagtagaacg gatttctaat ggcgtaatct tttgaaaacc ttctggggct
    27241 tctacttctt caagtaaata atccccataa ggaagatttt caaacttgcc ataaccatca
    27301 aaccctaatt gctcgttata agctgtggtc gcttcatctt cggcacctgt gatttcattg
    27361 gtcccttcca atggcgacac tttaaaggtc agatcattga agccagtttc ggtagtgccc
    27421 gcagctgatc cagcaaattt aaagaaatca aagccaaagc gaatcacttg ttcttttgcc
    27481 gtaacatttc gggtaatcac ggcatttttt tcgctatcat ccaccttttt gatggataca
    27541 ggatactttg tttcatcaag gctatatcct tcgggcgctt ttgtttcttg ccaataatac
    27601 tcgttgatag ctaaatgttt aacggcaact tggttctttt cgtctaaagt caaagtgacc
    27661 gtttcatcag aacctttcgt tcccttcgct aactctggtt taaaagcctc actccattta
    27721 acagcttttc catccttagc agtaaacaag gtgtattcag caccctcaaa gactgcctta
    27781 ccttgtgctt tatccccagt atctttgtct tctttcgtca aagtggtttc gccagtgact
    27841 tcttgatttt gcccttttac gttactggta acaggggcta cggtttgatt ggcatacttc
    27901 aattcgactt ttacaggttt gaacgtattc acgaacccat gactagcttt agtttcggtc
    27961 acataatagg ttcccaattc caacgcatta gcaatctctt ttggtgtttc cgcatgaccg
    28021 ttttcatccg tggtcatttc ttgaacgatt tcaccggttg gactgtcttt gcgaatggca
    28081 aacgtattac cagctaagga ataattgtca ttccaaagat cactccctgt ttcaactccg
    28141 cttttatcca gaatgatttg ccctttttcc cgtgcgtttt ttgaagtcac gtaaatggtt
    28201 tcgcctgctt taatggtggt agtcatgggg gtggtatcaa tcgtataagg cgccggtact
    28261 gatttttcgg taatggttac ttttgtacca tgaggaatct catccaatgt agcaatgcct
    28321 tctttgtcag tagtcacgtc ttttgcaggt aaagtttttc caaagtctaa atggaaaacc
    28381 gttcctggta caacatcacc cgattcttta tcgacttttt tgatctttaa agaaccctcc
    28441 gtttccacat caattttgat tgtgtaggta ttaggtttat caatcgcccc ggccattaaa
    28501 gtctgttggc cagccttttt ataggcgaca ggtgttccgg taccagcaga tttcttaagc
    28561 gtcagcacac cactttcttt tgaattggca tttggggtga ttatcaactg attaccattc
    28621 acacgataat caatgtttgc ggcattttcc acaacctcat cgaactctga caaattcaga
    28681 ttattcgtat ccgtcacagt agtcgattgc cctaatactg ttttagccgt actattatgg
    28741 aaggttggtt tcttctgata atcgtcaatg gcttgattta tttttgcctc aatggctgcg
    28801 atatttacgg cactaccatc tgatcgtttt attgagtgaa gggtataccc attaacttct
    28861 tgccagatca tcttctgtgc cacaatatga gtatctgcat ctgtacctac ttttttccat
    28921 aaaacggaaa ctaatttggc tttatcggac atatctggta acgggttttt ctcatagcca
    28981 ggagtgaact cattgtaaat cgggactcct ggttcaatac agaaaatatc ttgtttaaag
    29041 ccgttgtaca ctgcataatg cggaacagtg acttctgaca ttggacgacc atcactaaaa
    29101 gttccttcaa cagcatattg atgtgctaaa tctagttcta cggttaccgt ttgtggatgg
    29161 gtaatttcat cagcaaaagt aatcgtagga gcaattgctc caacaattgc ttgaacagcc
    29221 aaactcaaaa tagttaatac acataatttc tttttttgtt ttttatacat gaaatgttct
    29281 cctttcaaat taagcacaaa aaaataagat gctcgcttga acatctttgt ttataaagtc
    29341 tttttaaaat ttttattgac tgaatagaat ggatttgaga atgaatgagc ctaaaaattt
    29401 aatctaaaat acgaaatata ctgcctttaa attagaacac attttagaac acaaacattt
    29461 caaatttaat caaatataat aaaatatatt tacgggaaaa cctttataaa tcaaggtttt
    29521 tcaaacttat tcaatgatac gaagaaaaaa ccaggtctta agaaagcccg taaagcttca
    29581 cagttctcaa aacgttaatt gcgattatcg ttcgagttca cgagttacga gaatcgtact
    29641 gggcgaagca atgctgagcg cagttacctt aatcctaact tttctggcac tttccacaat
    29701 ttggaaagtg ccttttttgt gtttttagct atgttaatag gtaggattct tggtctataa
    29761 tatcattcga tctataaaca aacgggaaaa agataaagta tcatcaaaca atattattta
    29821 tccgcttctg aggttgtgac agaagtggtc agcttcaaga aataagaagg aatttacaaa
    29881 aattgcttct catgtttgat aaatattgtg acttgtgacc gaggagttgg caggagaaca
    29941 ccgtttattc ggaagaaggg attgtgaaaa gtcttttgtc ataacctctt atttggtagc
    30001 taattgtaaa tcccaaacgg catgtattat ttttcataag tccgttttta aggattaatg
    30061 ttttcttttg ctttttatat ggtctggctt cagcgagaag ttccaacgca tatacaagga
    30121 cgacttatgt caacgataac aacgattacc atggtggcca gtccacttgg attagcactg
    30181 tttggtgcgc tctttgattg gaagacaacg caaccacgtc tgcgagacag tctaattttt
    30241 ctgggaggga gtcttgctat agttatcctt ataaccggtc tggtaaaatt actcaagctt
    30301 gatctgaaag aagcaaaaat tttcagtgaa gaggaataca gagaaacgac tttggtcgaa
    30361 aagaaagaat caggaaactg atacaagcgt ataacaaact gcctgataat aggaaagaca
    30421 cgtattgtcc gattgttttt tttgacattc aattggaaaa tttacacttt ctttatacat
    30481 ttctggctgc tttctttata cccgtcaact atacttcact catcaataag aaagaaggat
    30541 aagtatggat tttggaaatg aaacggtctt gaaagcaact ggtataacca aaaaatacgg
    30601 tgctgccaaa gcattagata aagtatcaat cgaaatcaaa cgtggaatga tctatggatt
    30661 gattggggaa aatggagcgg gtaagtctac ctttatgaga actattatgg gtttgatctc
    30721 gattgatgaa ggttcgatcg aattatttgg aacgacggat ttgcaggcag caagacgacg
    30781 tatgggtcaa tcgattgaaa cccctgctct ttatccagaa ttaactgcaa gagataacct
    30841 acgtatccaa gcagcgaacg gcggtgtgtc ggatagagaa atcgaagacc tgctgaaaat
    30901 gatgcgattg gaaaatacag ggaaaaagaa agcaaagaat ttctctttag gtatgcgcca
    30961 gcgtttagct attgcaaatg cactgatcac gaatccagaa tttttgattt tggatgaacc
    31021 gacgaatggg atggatccag cagggatggc agaaatgcga gaaatcatcc aacgtttggt
    31081 aaaagaacgg ggcatcaccg tcttgctttc tagccacttg ctagatgaat tgtcacagat
    31141 cgccactcac tatggtattt tgcatgaagg acacttgatc aaagaattat cgaaagaaga
    31201 attggcacaa gaatctcgtc aatttataaa aattgacact tcggcgacgg aacaagcagt
    31261 aacggttctc gactctcttg gttataggga ttattttgta caatctagcc gtgttatcca
    31321 gctttttgaa ggaattgacc aagtagcagc catcaatcaa gcattggtag aagcaaaagt
    31381 tcctgtggat ggtattcacc tagttgggca aaaactggaa gattatttct tacaattaac
    31441 tggaggcaat ccacatgttt aatctattaa ctgctgaaaa aataaaagta tggaagagcc
    31501 gtaaaatgtg ggtctcacta ggactgatgg tcgtacttcc gcttttttat tcttggaacg
    31561 aatggtatat gcatacaaaa tacggcaatg aattaaacca agcaacggat acagtcatca
    31621 acggtgcaac agggatttta atggtagaaa aaatggctgc atggattctt ctggctttcg
    31681 cagcatttgc gtgcttttat attggggaag aatttcaaaa cggtacgatt cgcaatacgt
    31741 tatctttagg acggaatcga atgacttatt atgtgtcgaa acttctcgtt acgcttttga
    31801 tcacaacact tggcgtggtt ttgatcacgg gactagccat gcttgcttac acacttgctt
    31861 tcggatttgg tgaagttgag ggaatcaaaa actatggaaa ttatgttcta aaagtatttc
    31921 ctgtattgct tctgctgaca ttagggacat tatctgttcc agtggctttg acattcatca
    31981 ctagaagtac cagtgtcagt ttgcttcttt cttttcttta tatcatggga acggccttca
    32041 ttcccggtgt tttcgcaaaa atcaaaggct tggaatttct aacggagtgg tttacggaaa
    32101 cgtggctcat gtatacggac tttgcgcaac aagcaactta ttcgcaagca ccgaaaatga
    32161 ttctggtcag tctagtaaca atcgtggtat catcagctct aggaatgttt attttccaaa
    32221 aatctgacat taagtaggtg gtcctgctgg aaactatttt gattatcgaa gacgatgaag
    32281 ctgttcattc cttactggag gaggttcttg aacagagata caaacttctt gatgcttatt
    32341 caggtacgga aggaaaatta ttgttaagca catatcctgt cgatttgatc cttctagatc
    32401 tcatgcttcc agggctttca ggtgaagcat tgttagcaga aatccgacag acatcgaatg
    32461 ttccgatcat cgttctttca gcaaaaagcg atcaaagaga taaagtttcc ttgttggcag
    32521 caggagcgga tgactatgtg acgaaaccat ttgatatcga agaacttttg ttacgaatcg
    32581 gcatccaact tcgtcatcag acttctgtcc aagtaaaaga tctgtcgcaa cgcatctatt
    32641 acaaggaaat cttactggac aaagagtcga gggatgtaaa tgtgaatggt gtggctgtcc
    32701 atttgacagg aagagaattc gatctgctga agcttttttt agaacatccg aaaaaagtat
    32761 tttcgcgagc aaatctttat gaaacagttt ggcaagaacc atattttgat agtgagaaaa
    32821 cagtgaatat gcatatttcc aatcttcgag ggaagctagc aaaggcacat gctgtgtata
    32881 ttcatacggt gtggggagtg gggtttagat ttgattaaat ggctgttgct tctcgttgtt
    32941 atctttatgg gaagttttat tttcttcctg caaagggaaa tcaagagatt aactaagaaa
    33001 atcgccactc tttctgaacg acaagaatat ggtggtcgtc tttcagtcga atttcgagaa
    33061 aagaatctaa tggacctagt agatgcactg aatacatggt tggatgaaaa tgaacaaaaa
    33121 ggacaagcat ttcaagaaat ggaagaaaac atcagactat cgatcgtcgg cctttctcat
    33181 gatctcagaa ctcctttgac tgctattaac ggttatgtac aattactgga acaggtagag
    33241 gatacggaga aacgggagaa gtatttaacg atcatcaaac aatctgttgc aagattactc
    33301 agtatgacgg atcagtttta tgatcttgct cgaatagaga cgaagcaaaa agaaatggat
    33361 ctccatacgc tttctttttc taaagaggtg gaagaagtat ttttctcttt ttatgaacaa
    33421 ttttctgaga aaaatatcga ggtagcgttc gctgaacact cgataaatac aactgtatta
    33481 gcagatccgc tcatgttgct gcgagtgctg caaaatatca tacagaattt attgcgttat
    33541 gcagatggac aagcaaaggt gacattttca aaagaggatc cattcattcg attaacagtg
    33601 gccaatccta tcaaaccagg aacgcaaacc tctgttgaaa aagtcttcca gcgtttttat
    33661 accgaaaata ccagccgcac aaatacggaa tctagcggaa tcgggcttta cctgtcaaaa
    33721 aaattggtgg aaggaatggg aggagagatg accgctagat tagacggggg catcttctcc
    33781 atctccgtca agttgcggag ggtttaatta tacaagatta gtgagaacta gtagaaaaat
    33841 gtattatctt atatatattt aatagagaaa aaagcctgtg atataaatat tgcaggcttt
    33901 tttattgatt tgaaataatc aagaatatta gaaacttcta cattaatcaa gaactttctt
    33961 ctgtatgaaa atagagataa taattgtaca cttaagctgt attcaggagg tgaagataat
    34021 gggcatcgaa tttagcgaac ggcaaatgaa aatactatac tttttgattg aaaatgagca
    34081 cacaacttac aaagaattat cagaacaatt atatgtatct acgaaaacaa ttagcaaaga
    34141 tattttaatt ttgaaagaaa taatccaaaa aatgccagat gtacaaatgt tggttcaacc
    34201 taaaaaaggc gtttcgctta ttggaaaaaa agaaacactt cgaaatttat tatccagtca
    34261 taaaatcaat atcgcagact ctccggagga ccgtctaact tatgtgtttg ctaaattatt
    34321 gaatactaat gattctctaa agattcaaaa tcttgctgac gaattatacg tgagcaggtc
    34381 gacggtagaa agcacaatga aagatgttag aaaaagacta aacaagcatg ggatgtcctt
    34441 agtttcagat cgaaatggcg tgcgcatcaa agctgatgaa aaaagtaaaa gaaggttgat
    34501 gtcagaagtt gtgaactatt attggggtgg ctttacagca tctaacagta aaacagatct
    34561 cttagacttg aatatcaatt tcattggtga agcgaataat ttcttaaata aggacatatt
    34621 gaaaaaagta gccgaattgc ttaatcgata catgaataag acacaattga aatttacaga
    34681 ttatgaattt caaactttag ctatccacct tgctattgcg atagaaagga ttgaaacaag
    34741 caatttctta tcacgtagta ataattctga agaagaaatc tcaaagaata cacttatttt
    34801 aatcaaaatg attgaaaaag cattttcaat tattgtgcca atagatgaac aagcttatgt
    34861 tgacattcac attacttcga ttgaaaaaaa tgctatgaat cacgacttag atcaaacgaa
    34921 gctttcctat ttagatatgg gtgttgagct acaagagtta gtaagaatta aattaaatga
    34981 attttatcct gatgaagaat tgatcaataa tctagtatta catctcaatg cagcagttaa
    35041 aagactcaaa ttgggagtaa atatctataa cccttatacg gacaaaatca aatattcttt
    35101 taaaaggtca tttatgataa gtgtagattt attagaggaa atcgaagaga gattctgtat
    35161 acattttaat gaagatgaaa tcgcttacgt aacactccat attcaatcac ttttggatcg
    35221 ttacaaacct gacaaaacga aagtgatatt agtttgtagt agcggatatg gaacatcaaa
    35281 attattagag caaagaataa ataacggttt tgcagatatg gttgagatag aagatgtact
    35341 aagcatcaat gaactacaag atagaaaagt cactgatgaa cttgttataa gcacgattcc
    35401 gattgaaaat acaaattttc ctgttattgt tgttagtcct ttgatgttag gggcagatgt
    35461 cagaaaagta aaacagtatc tacagttaaa tgaacaaaca gaagatgctt ttctaaattt
    35521 attagataaa cgattttgct tttttaccca ccaaataaag gataatcaag tgaacgtttt
    35581 aaatataatt accaaaaaat tggtaaaaga aaattacgcg aaagaaggga tactagaggg
    35641 agcaatcaaa agagaaaaac tatcttcaac agcgatgaat gattttgcta tgccgcatat
    35701 cgaagtagat tttataaaaa agccagtaat ctgtgtctac gttaatccag aaggaattga
    35761 ttgggatggg acacttgttc attctgtttt cttttttgca cttaatgaaa aagtaaaacc
    35821 tgaattgaat aaaatttatg aaacattcaa tgaaatcgct tcgaatcatg agttattagc
    35881 aaatttaaaa acgagttcgt cgtttgaaaa attagtttat atattgaaag aaggcttata
    35941 aatgatcagt gaaaaaatca atataaataa tatagtgata gatttaaatg catctgataa
    36001 aaaaagtgta gtcgaaaaat tatcgaaatt gttagagact aatgggtttg tgtctgacca
    36061 caaacaattt caaaaagacg tatttgacag agaaaaccat atgaccacag gaattgggaa
    36121 aaatatcgcc attcctcatg gcaaaagcct agcagtaatt gaatcaacag tggctgtagc
    36181 caagttaaag cggtctgttg aatggggcgc attagatgat caaccagttg atctcgtctt
    36241 tttgttagca ataaaagaca ctgataaagg tactaatcat ttacgtattt tagcagaatt
    36301 atctggaaga ttgatggatg acgagtttgt taaaagaata aaagattcga gaaataaaaa
    36361 agaattggta aaagctctat cattttaaaa ggaggaattt catatgacaa aaatagtagc
    36421 agtaacagca tgtatcgcag ggattgccca tacttatatg gcaaaagcta atttagaaaa
    36481 gtttgcgaag aagaaagggt tagatattaa agtagaaaca cagggggcta tggggataga
    36541 aaacaaactt tcaatttcgg atgtttccga ggcagatgtt gtcatatttg cagtagatac
    36601 aaatgtttca gaacgaggtc gctttgacgg taaaccgatt ttaacagtag ggacctcaga
    36661 ggtagtaaaa gatggtgaag agacgattga tgaagcaatt gcattaatta acaaataaaa
    36721 aatatatcgt gggggagtta ctaatggaat tcaagaaaca aggaagagaa attctaaatc
    36781 atttccaaag tggggtttcc tatatgatcc cgcttgtcgt agcagcaggg ttactaactt
    36841 ctattgcagt catttttggt ggcacaggtg tttgggacca gacggataca ttctggggag
    36901 tcctgcgaat gattggtcag acgggattac agtttatcgt accgatgatt tcagcctata
    36961 tcgcatattc aattgctgac cgtcccggtc tagcaccagc ttttatcact gggatgatgt
    37021 gtcaaaatct tggtatggga tttattggag gaatggttgc tggattagct tgtggctatt
    37081 tagctaatgc attgaaaaaa gtaaaagtac cgatgcaagt tattagtcta aaatcaatta
    37141 tgctgattcc ttttgttact acattagttg ttggaatcat attatggtat gtactagcta
    37201 cgcctattca agcaatgaca acaggactga ctaattggct agaaggcatg tcaggagcaa
    37261 atgctgcttt gatgtcggct attttaggag gaatgatggc gtttgatatg ggaggaccta
    37321 tcaataaaat cgcatatgct tttggggttg cttctttttc aagcggaagc tttggaccta
    37381 gtacagccat gttattagga attgctattc cgccatttgg aatgttttta gcaacaattt
    37441 taaacaaaaa actttatgat gaatcggaac gagataatgc aaagacagct atgattatgg
    37501 gccttgtggg gattactgaa gggacgatcc cgtttgccgt gaaagatcct ttacgagtaa
    37561 taccaagcat cgtggtgggc actgcagtag catgtggtct aaatggattt tttggtgtta
    37621 ctcaagaaac aatgctttca acctttatgg caattccttt tgttacgaat atacctttat
    37681 atttagtatc aattgttatt gggggagtgg ttacagcgtt aatggtcaat gcactaaaat
    37741 ccatcaagta taaaaaagaa caacaagtaa attcagtaga agaaggaaag taggagaatc
    37801 tgatatggta aatgcacata taattcaaca cacgcactgg gacagagaat ggtattttac
    37861 aaatgaagat gccatcgtat tgtctgatca ggtctttact gaagttctag atgaattgga
    37921 aaggaaccct aaagtaaact tttgtttaga tggacaaact tcaatcgtcg atgagtattt
    37981 ggagatcaat ccgcaaaagc ttaaggttat ccaacgactg atgaaaacag gacaactatt
    38041 cgtgggaccg tggtatgcac aaacagatgc cttattagtg gatgctgaat caattttaag
    38101 aaacttaatg attggaatca gtgatacgat ccttaaatat ggagatccaa tgatggtagg
    38161 gtatttgcct gacacgtttg ggttcaatgc gaatttgcct acacttttgc atcaagtaga
    38221 aatcgataat ttcatgtgtt ggcgaggatt aaactttgaa acgatggttc catctcctta
    38281 ttttatctgg aaaggattgg gagataaatt tgtttatgcc atgaattttc catttggtta
    38341 tatgactgga atgatgacct tagacgcaat taaagatatt cctgaatttg caaaagaaaa
    38401 gttggatgaa gcagttgaaa tgcttcatat gcaaggaaaa aacaaagata ttttagtacc
    38461 ttcgggtgta gatcaaaagt caatgatttt aaattttgat caagtcgtag agaagttgaa
    38521 taaatacagt aaattccaca atattatttc agattatcct tcttatgtag aagttatcag
    38581 aaacaaaaaa gaattacctg aatataaagg tgaacttcgt cagccggttt actctcgtgt
    38641 tcatcgttct attggctcag taagaacaaa aatgaaaata gaaaatttta aattggaaca
    38701 aaaaattcta aaacgagttg agccgctcat gctgattgca gaaagaaatg gtatccatat
    38761 cagtaaaggc ctacttcgtc ggttatggaa aaagattttg caaaaccagg ctcatgatag
    38821 tattggtggc tgtgtttcag ataatgttgc ggaagatatt ttccatcgaa tcaaagaagc
    38881 taatgagata gctgacggaa tcgaaaacct tattgtaaaa cggatggctg atgcattaaa
    38941 gctaaaatcg aatgaagtcc ttgttttcaa cacaacgact gagaattata gtggatcgaa
    39001 aatcgtgcat gtagtagctg attcaaagaa aatacacttc aaaggtagtc cctataatat
    39061 cattgaaaaa gaagtttatt ttccagaacg gaaaaatgct tatcggatga ttgctacagg
    39121 tttcgaatat tttgatgaac cagcttatta tgaacttgac ataaaaataa atgtaaatgt
    39181 gccagcacta ggatacactg ttgttgaatt tgagaaaaat aatgaaaaat tagaaacagc
    39241 aatcgagata gacaaaactg agataagtaa caataaatat aaactttctt ttaaagatgg
    39301 gcaattgaat ttgatagttg gagatagaca atatcttgat ttcgttcatt tgatcgattc
    39361 agcaaatgat ggcgatacgt atgattattc accattagaa ggagatacag aactatctct
    39421 aaaattagaa acagctaaag tatataaaga ttctttacaa gaaacattag ttgtttacgg
    39481 aaaagctcaa cttcctaaag atttaaaaga tcgtttatct gaaaaacccg aaatggaaga
    39541 aatatcttat gaaatatcgt tcagtctggg agaaagtcaa atcgttgaag gcacactcaa
    39601 aattcataat actgtttact cgcatcgtat gcgcttagca attaatgttt tagataaatc
    39661 tgaaaaatca attgcacaaa ttcaaaacgg atttattgaa aatacccctg atgtcattcc
    39721 tccgaattgg tcagaaacta tggtagagaa acctgttaat ttagaaatat tcgataagag
    39781 tgtctctgtg gcagaaaatg atcattattt tacgttttat gcagatggtt taaaagagta
    39841 tgaacgtata gaaaatcaat taatcataac cttattttca acaacagggg aacttggaaa
    39901 acctaatctt gcttggcgac ctgggcgagc ttcaggagat acgacaaatg aagggcatgt
    39961 catgatggaa acaccgctag ctcaagagat aggtgagtat aaagtcactt ttggatttaa
    40021 tgtagaagaa ggaagattta atgagttcaa agtggcaaaa caggcagaaa aacggttaga
    40081 acaatctatt tcttatcaaa agcagaaact gaatgtattt attcacaggt tagataacaa
    40141 gatttggcca ttacaaaatg aacttgatgt tcctcgtaaa ttttcattga ttgaattacc
    40201 tgaagactta attgtatcat cgatttatcc ttcttatttt catgaacaag gatttgttct
    40261 tcgcttagct aatccaacag aacaagaaaa aatagtacct gaggctatcc tttcccttgg
    40321 gacagttgtc aatgcactgg agaataaaca agaattaaca acaattcctc cctatgatta
    40381 tttgtcaatt ttgataaatg agagataaat atgtaatata caagaaaaaa acacctttta
    40441 ttttgaaagg tgttttttta ccctcttttc aaatagcgca acagtgctat actaaagcta
    40501 acaaataaag gaacggagag ggatatgggt caaagagaat ttatcgtttt aattattatt
    40561 gcagctgtta ttttattagt tgctttagac attttttcac gtttgaaatt aaaggaaaca
    40621 gttcgtagca aatgggaaaa aattccttat caaccgagat ttgataaaga agaaagcttg
    40681 aaggaagcat ggctcacgga gaagaagttc cgttcatggg atagcgaaat cgatgatctt
    40741 acttggtatg acctggatat gttcgaagta ttcgaaggaa tcaacagtac ctattctagt
    40801 gtaggttcag aagcgttgta tcaacgttta cgcagctttg attttggtga agatcagcag
    40861 ttagaaaaac tgattgcttt ttatcaggaa aatcctcaac tgagagaaaa aatccaatat
    40921 caattcgctc gtttaggaaa aaaagatcat aattttgcaa aacaatatct agcggatgga
    40981 aaaagtaaaa agattggacg cgtaggttta tttatttttc ttggcttgct tccgctggtg
    41041 ggtttgggtt tgcttctttt aggacaagta ctaggaatct atttggcaat cggtgggtta
    41101 gtattcaata cggtctatta tctagtgaag aaacaaacac tagaaacaga gctgaatagc
    41161 atgagttatt tggtttcaac gatttcagcc gccaatcagc tagcaaaact gccgacacca
    41221 cttcaagaag agctgaaaca gcaaactaaa ccattaagag acatccctaa attaggtttt
    41281 tcatttcgta tgaagagtgg gagtgaagca gagttgattt ttgaatatgt caacatcatg
    41341 ttcatgctgc catttatctc ctatcatttc gttctcacta aattagaaaa gcattcaact
    41401 gaagccatca agctgtggga attgctggga gaattagaag tagccgctgc aatattgaat
    41461 taccgtactt atatgcccat cacttgccga ccagagttta ctgagggcgg tgtgacagcg
    41521 aaagatatct atcatccatt gttaaaagaa gcggtagtga atccggtcaa ctggcagaag
    41581 aatacattag tcacgggttc caatgcatca ggaaaatcta cgtatgtcaa aagtatagcc
    41641 atcagctgta ttctagcaca gacgatccaa acagcgattg cagaacagtt taccctgcaa
    41701 cctggacatg tgttgacttc catggcaatc gaagatgatt tatttgaagg cgacagttat
    41761 tttgtttcag aaatcaaatc gatcaaacgt ttgttggacc aagtggcttc caaagaacgc
    41821 tgctactgct tcgtagatga gattttaaaa gggacaaata cgatcgaacg gattgcttct
    41881 tccgcttcag tcgttcggtg gcttgcggag tatccatctt tagcatttgt cgccacacat
    41941 gatatcgaat tgacagagat tttaaagaat tattgtgaga atgtccactt tgaagaacaa
    42001 gtaacagaag gaaaaggaat cagttttgat ttcaaattaa aagaaggacc ttcaaggacc
    42061 cgaaatgcga ttgccttgct gaaagttttg gattacccag agactttagt agaaaccgca
    42121 aaaacggaag ccgcgttatt tgatgaaaag cggcagtggc atgtattggg ataggcgaag
    42181 tgatcgaaag tatttattgg atcaaaaatc ttggcttttg cttccgccgt ttatacgtgt
    42241 ttagagcgtg agacaaaagt gttctttact tttgtctcac gctccttttg ttgcacatat
    42301 gaatagtaat cctttatctt cgatttgttt tttctgaaaa ggttgtctga cttcgtccga
    42361 ttgatgtctt tcattaggag attttcctga gcccagcaac ctgatttttc gctggaacca
    42421 agttgttttt tcatctgctg tattccatga tacaataaag aatcatgtct ataaggagat
    42481 aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaaggttta tggaagtggg
    42541 gtcgaagcat tgcgaggaat cgatctttcc gtgaaagaag gagatttcta tgcgctatta
    42601 ggaccaaatg gagctgggaa gtctacaacg atcggaatca ttacttcttt agtcaataaa
    42661 acatctggaa aagtgaatgt cttcggttat gatctggata cggatctagt ccgagcaaag
    42721 caacagattg gattagtccc gcaagagttc aatttcaatc cctttgaaac ggtccagcaa
    42781 atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat gaaacgcagc
    42841 gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc acgtatgctt
    42901 tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga accacgtttg
    42961 ctgatcttgg atgaaccaac agcaggagtc gatattgaat tgagacgcga gatgtgggag
    43021 tttctccgag aattgaacga aaatggtaca acgatcattt tgacgacgca ttatttagaa
    43081 gaagctgaaa tgctttgtcg caacatcggg atcatccaat caggtgaact gatcgagaat
    43141 actagtatga aagagcttct atccaaactg caatttgaaa catttatctt tgatttagcg
    43201 ccatatgaga ccaaacctgt gattgaagga tacgcttgta catttgaaga tgaacagaca
    43261 atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca gctgacaaga
    43321 caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga actgtttttg
    43381 aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt attttactgc
    43441 attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatctggg tccaaacact
    43501 ggttccgcct gtgatcacga catccttgta ctttgttatt ttcggaaaaa tgatcggtgg
    43561 tcggatcggt gacatgggtg gtttttcata tatggaattc atcgtgcctg gtttgattat
    43621 gatgtctgct atcactagtt cgtatgccaa cgtttcttcg tccttcttct ctcaaaaatt
    43681 ccagaaaaac atcgaagaaa tcttggtagc gcctgttcct acgcatgtga tcatttgggg
    43741 ctttgtgatt ggcggagtgg gtcgcagtat tctcgtcggc tctctcgtaa cgatcatttc
    43801 cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta cattattgat
    43861 gacggcaatc ttgttttcgt tagcaggact gctcaacggg atttttgctc agtcttatga
    43921 cgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag gtggcgtttt
    43981 ctatgccatc tctatgttgc cgccattttg gcaagctgtt tcgaaagtca atccgatcgt
    44041 ttatatgatc tccggtttcc gttacggatt ccttggaaca atcgatgtgc cagtagcaac
    44101 atcgatgatc gtacttgtgc tattcatcgt tgttttgtac gccgttactt ggtatttgat
    44161 caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg ttttgacctg
    44221 agtattagag aaacaaacgg gaaaatagtt cctcatattt tgtcgtttgt tgagctaata
    44281 ccgagggtca gctgattgaa caccgtttag tggagaggtt gtgaaaaagc tgttctgcat
    44341 caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gttggactta
    44401 ttaccgcaga tgcaagcttt tgaacaccat ttattccgga aaaggaattg tgataagatt
    44461 tttgtcacag tctttttttg ctcaatcaga aacatctttt aagctgttca ctttgcttct
    44521 ttttttgttt cctagtataa tgacaatgtt tttgagaaag attgcgactt cactgcttga
    44581 tagaatggag agtaacaaat aaatgactac taatttttgg aaagagctgc caaagccttt
    44641 ttttgtttta gcaccaatgg aagatgtaac agatgttgta tttcgccatg tcgtaaagca
    44701 tgcagcagca ccggatgtct tctttactga gtttacaaac tcagacagtt tctgtcatcc
    44761 agatggaaaa gacagtgtac gaggaagatt gacctttaca gaagatgaac agccgatggt
    44821 tgcccatatt tggggagaca agccagaatt ttttagagaa atgagcattg ctatggcaga
    44881 aatgggcttc caaggaatcg atatcaacat gggctgccca gtccctaatg tagcagaacg
    44941 tggaaaaggc agtggcttga tccttcgccc agaagtagcg gcacaattga ttgaagcggc
    45001 taaagctggc ggtttgcctg tcagcgtcaa gacgcgtatc ggctatacgg aaatgtctga
    45061 gatggaagaa tggatcagtc acctacttca tcaagatata gccaatcttt ctgtccattt
    45121 gcgtacgcgt aaagagatga gtaaagtaga tgcacattgg gaagtcatcc ctaaaatcat
    45181 tgctttacgt gaccaaatcg ctccgcaaac actgatcacg atcaacggag atatcccaga
    45241 tcgccagaca ggagaagaat tggccgaaaa atatggcgta gatggcatca tgattggccg
    45301 ggggattttc aagaatcctt atgcatttga aaaagaaccg agggaacata cagaaaaaga
    45361 actgatcgga ctacttcgtc tgcaactaga tctgcaagat cattattcag aaatcatccc
    45421 gcgttccatc acaggactac atcgtttctt taaaatctat atcaaaggat ttccaggagc
    45481 aaatgacttg cgagttcagt tgatgaatac gaaatctact gatgaagtac gagagatctt
    45541 agacaccttt gaaaaagaac atccaacgct tttcagtgaa caatgatttc ttgcttgaac
    45601 accgtgaatt agaggtcgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca
    45661 aaaatagctc ctcatatttt gtcgtttttt gagcgaatac cgagggtcag ttgattgaac
    45721 accgtttaaa agaggttgtg acaaaagtct tgtcgcaacc tcttttttaa ccaaaaaagt
    45781 gcaaatagtt tactttaagt aagagaataa ttatgatgaa taaaagtgga acaaatagca
    45841 tactatatat attgaaatga aaactgtatg gatgctccaa cttgatgaag ctaaggagga
    45901 attcgtatgt cagaaaagga attaatgaag gtagtcggtt tttatgaagg gttgtcgatc
    45961 gatgatccaa aaagctttat tgatgaaaaa gaacagatac ctgtacccag ttcacgggat
    46021 cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg atacgaaact tcgccaagat
    46081 aacgggataa gaaatgcttt acgtatctta gggtttgatg gagtcgggaa agttgttgct
    46141 gtaggtgatg aagtgcaaaa attttctgtc ggcgatcgcg ttttttacgc aggtacaacg
    46201 acaagagcag gaagcaatca agaataccaa ctggttgacg aacgtattgt cgctcttgca
    46261 ccaaaaaatc tttcagatga agaagccgca gccttgccgt tgacctcatt gaccgcttat
    46321 gaattgttat ttgaaaaatt tggtctgact ccagaagaaa atgccaaccg cgggaaaaaa
    46381 atcttagtga tcaacggagc tgggggcgtc ggttcgattt tgaatcaact agcgcattgg
    46441 gctggcctag aagtctacgc gactgcaagt ccgaagaatt ttgaatggct gaaaaaaaca
    46501 ggtgtagact atccaatcga ttatcatcac gacttaagta aaagttttcg tgaattagga
    46561 atcgatcaag tagagtatgt cgctgtatta ttcgatatta cccgctactt tgatcaaatc
    46621 aaacaccttg tccgaccatt tggacatgtg ggaacgatcg tagggatcaa agagccgtta
    46681 gatataggtg tttggaaaaa ccaatctatc agcttcgact gggagtatat gttcgctaaa
    46741 acagactata actatgaaat cgaaacgcaa ggagaagctt tagcgcacat tgctgcttta
    46801 gcaaatgaag ggattatccg cagtacattg agcaaggtct attcagatgg gatgaatgcg
    46861 aagaacttga aacaagcaac caaagacgta gaaacaggac atatattagg aaaagtcgtt
    46921 gttagtggtc cgttcaacgg ataagcaaga atacgccgat ttttttctta tttgactttg
    46981 ataaaaacaa gagcgcatga cagtaatttg ccataagctc ttgttttttt atttataata
    47041 agcgaatcaa cgaaagggaa agaattccta ccaagacgat caccaataag ctttttgaaa
    47101 gaatagcagc taaaagtgta ggaactgaag ccaaagcatt ttcgagattg atctgcggca
    47161 gatgtccgat attttgggta aatagactgc tgaaccataa agcagacata atgacgatcg
    47221 ggacaaaact taaatactca agcaacggtt gcggcaaatc gaattttttc agtaagacaa
    47281 atggtaggat cctagaaagc caagttgcta aagtacaacc taaaatagtc agcaatgtaa
    47341 actcaaaaga aggcatgttt gatccacacc cccaatccgc acccaatcag tgttacaact
    47401 aaaatcacta agttacttgg gataaaaatc agtccgacat ataccagaat caaagtgaga
    47461 ccaatcagga tcaactgtaa tcgtttagag gtgtttctat cagaaatgac ttgaagataa
    47521 agtaacccga taaacatagc gacgatggcg aaatccaaac caaacttttt cggatcagta
    47581 ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca cccacgtcaa ataagaaatc
    47641 caattagctg tattcatcca acgaaagttt aacttttgtc cagtatagtt caatttattc
    47701 attcccagtg cgaaactttc atctgtcagc aatgtgccaa tcagcagatt ttgaagcagc
    47761 cgatttttct tgaaataagg agcaatcgtc atactcatca gaatcattcg ggaattgact
    47821 agaaaggtcg agagaacgat ggagagcagt ggactatggc tagcaagcat gctgaccgtg
    47881 ataaattggg cagaaccagc atagatcagc agggacatca acgtgacgac cagcggatga
    47941 aacccggcag ctttcccaac gataccaaat gcaagtccaa taccgatata accaaaaacg
    48001 gtaggtaacg tgtctttgat cgcagttttt atatctaact tttcatccaa taacaaaact
    48061 cctttagcac gacataaatt ttcataattg tcgtcaaact tgtgaatatt ctaaattttt
    48121 aatgaagact tgtcaacttc aattttaaac aaataaaaaa gactggacgt agtttaaaaa
    48181 aatcttctca agttagattt ccagacctaa cttctgagga tcactacatt tgtcacagtc
    48241 tcttatactg acaagtataa atgaagtatg aagaaaaagt atttgttatt ggtatttctc
    48301 attattcttg ttttctaatg tgttcacttc aagtggaatt ttagaatatt attcaactaa
    48361 caagtctaca tcgtatctaa atccatttgt atcacttaat tgcttgtaaa attcgccact
    48421 ttttttaatt gttctttttt gagtagtaag gtcgagtgaa accagcccat atcggttttt
    48481 ataggcgttg atccatgacc agcaatcaat aaatgtccaa agatgatacc ccttaatgtt
    48541 acatccttca tttattgcct tccataagta agcaagatga tctttgatat actcaatccg
    48601 ataatcatca attatttgcc ctgtatcatc catgaaacgt tcttcatccg caacgcccat
    48661 tccgttctcc gagataaatg cttcgatgtt tccataatgg ttcttgatat ccatgcacaa
    48721 atcataaatt cctttttcgt aaatttccca tccccggtat tcgttcattc gtcgtccagg
    48781 catgatatgt ggttcgaaaa accactctgg taaaaatgga ctagatgtat ccaccataga
    48841 agaacgagat ttgacgcgac gtggctcgta atagtttagt ccaagaattt gagcggtatt
    48901 ttcgtagata catacaagat cttctttcgt ataatcttca ggaagctgat tatattcttg
    48961 taataacagg ataagctttt tagaaaaatt ccctttaacc ataggatcta aaaagctccg
    49021 cgtataaaat aaatcagcga tttctgctgc tttcacatca gcaggatttt gactacgagg
    49081 aaaagtagga ctcaaattta gaataacgcc tattttacta ggaagagcta gttctttaaa
    49141 tacttgtatt gcttttgcgt gtgcaagtat agtgaagaat gctgcttgcg ctccgcgttt
    49201 aaagtcaatg aggtttggat aatgaaaatc ttcaagataa gtacctaaaa taggcccaag
    49261 tggttcattg aaggtaaacc aatgacaaac taagtcacca aattctttaa aacaagtttc
    49321 agcgtaatga acataagcat caactacttc acgtgattcc catcctcctt tatcttgaag
    49381 agtagcaggc atgtcaaaat gatataagtt ggcaaatact ttgattccgt tattattcat
    49441 ttcggaaaat aattttcgat agaaagaaat ggctttggga tttacttttc cggtaccgcc
    49501 atttggaaac attcgtgccc aagaaataga aacgcgaaag gagttatgcc cagttttttt
    49561 cattagttct atatcttctt tatattgatg gatgtgatcg gttgtaatag aggaatcgat
    49621 tcgttgataa aaccggtaag gttgttcttt aaaccagcga tcccagatag tctctgcttt
    49681 tcctgtttct ccttttcctt ctgcttgctc agctgaccaa gcggaacccc accaaaaatc
    49741 atctggaaaa gttagctttt tcatagttgt cataattttt ttacctcctg tttccactta
    49801 atgataacac cgcttacaac ttaaagtaaa tagttttgtt taaatatata tatctttttt
    49861 attgtgtgta taattttgat gtggatttta tcggtttatt tatgttaaag tatagatgaa
    49921 gatatgtttt tgattggagg gtcaccattg aaaaaaattg cgaagtatat ggaaatttac
    49981 ttagatataa agaaaaaaat tgtaaatgga gagtataaaa ttggtgagaa acttcctaca
    50041 ggagatgaat tggcagttat ttatcataca agcaagttaa cagtaaagaa aggcgtagac
    50101 ttacttgttt ccgaaggagt tttgcgaagt agaagtgggt atgggactga agtcgtcaga
    50161 acaccaattg ataactcacg agttcttggt ccaaacgaag gtttgtttag cgtggttggt
    50221 gaagaacacg tagattcaga aatccatact ttctcaattg aacttccatc gaaaaaagta
    50281 gctgataaat taagtatcag tgtaaaagac tatgtatata atattgtacg aagccgtttt
    50341 attgattgtc aaccatactc gattgagcaa acattcatgc cattagcagt cataccaggc
    50401 ttggaaccaa aacatttaaa gaaatctgta tatgcttata tcacaaatga actgggtctg
    50461 gaaataaaga cttcgcacat acgtatcaaa ggagatactg cgaatgaatt tgacgcaaaa
    50521 atattaaaaa tagatccagg tgaatttatg attgagatag acaaagttgt tgctctatcg
    50581 tctggtaaac ctttcgagta ttcggtatcg agacatttat atcaagactt tgtttttgaa
    50641 gctgtttttg ttgaaaaata agctcgggaa aattattttt tcagagctta tttttttata
    50701 ctaaaaaaga tatatatatt taattaaaag tatttacttt ttttgataag tgatgtatta
    50761 tttgggtgga aaaagtaatc gattacaata agcataaagg agaaaaaata tgggcaaaag
    50821 tagcaggaag gacattgtat ttgaaaaatt tggtatagtt gcgactaagt tggggaatca
    50881 gatccattta agaactttgc gagatgcgtt tgctacattt atgccattta tgatgttagc
    50941 aggatttgtg acacttatta attcagtgat cttagaacca acaggattca tgggggaaat
    51001 tgtcgatact agtacattga ccacgttaca acaaattggt acttcaattg ccaatggtac
    51061 attaagcata actacattat tagttgtagc agcagtatct tatcatatgt gtgcaagcag
    51121 aaattataca aatcatattg cagcggtatt agttgctatt tcgacatttg ttgtattaac
    51181 acctatgcag atgatgttta ctcctgaaaa tgcggataaa gctgttgaag tgacgggtgt
    51241 gatacctata tcctatacag gagcttcagg aatgtttgta ggtatatttg ttggattagc
    51301 ggctacagaa ttatttatta aactttctaa taataagaaa ctacagattc agctatccgg
    51361 aaatattcct cccgctgttt taaagtcttt taatgtatta atcccaatca ttattacgat
    51421 tacctcgttt gctttattgt catttataat cattcaattg tttaatatgg atataaataa
    51481 gctaattacc acgatagtta cagagccatt aagtaaaatt acaactggtt taccaggatt
    51541 tttgttaatt acatctattg ctaatctatt ttttggattt ggtatccatc aagcagtaat
    51601 atcaggttca ttgttagatc cgtttttgat tcagaatatg caagaaaata tggcagctta
    51661 tgcaaacaaa gagcaaattc cacatattat caatatggcc tttaaagata cctttgcggt
    51721 aatgggtgga tcgggaaata cgattgcttt attgattgca atatttatct tcagtcgtag
    51781 aaaagattat aaggactttg ctaaattatc ggtaactccc gctatattca atataagcga
    51841 acctattatt tttggattgc ctattgtatt taatatcagt ctgattattc cctttgtgct
    51901 tgcaccgatt ttttctttga cagtagcata ttttgcaact gctgtgggat taattaatca
    51961 tgtagtagtg caaattcctt ggactacgcc accagttata tcgggatttt tagcaacagc
    52021 cggagactgg cgagcagctg ttttacaaat attaattata gccgcaagtg tgtttatata
    52081 tttaccgttt ttacgaattg acgaacacgt gacaactaaa ttaagtgaaa atgaaacata
    52141 atttactatg agataacaaa aattaacaca agaagtcgtt ggaatataaa tagccagtaa
    52201 tttcaataag gatcatattt tttgataagt acgaacatgt agaaaaacaa acattttttg
    52261 gtaaggataa tcattatttt tcacaagaca aacctatctg tgtatatacg tctatttagt
    52321 atatgcaaag ataggttttt ggtggtgaaa aataaagact gttaatatta ttaatcgtta
    52381 ttttaatatt tatctttaaa gattagagtg gttcgacaca cctgaaagtt gttgtcagaa
    52441 ttttatccaa tatacagatg cagtggaacc tctcttacaa aatgtagaaa agtatcataa
    52501 aatctatcaa caatgccgga aaatctaccc agcaacgaag gaaatttgtc atgacttata
    52561 aaacaaaacg cgtacctaat agtcaaatga ctaatattgg gtacgcgtta agtcttcttt
    52621 acttcatcct ttgcatgatt cgtgttttta aagcaaggtc tgctgtattt cttgtggatt
    52681 taggatggtt gttatcgttt acttgctatt tttcattgaa atcgtctttc tttaccgttt
    52741 ctttagtttt tttatctatt tttttctttt ggttagaaaa ttctttttcc cgaaagtctt
    52801 ctttcaggat ttttatggag tagcgttttg aataagaggc gtcagtatga tcgacttggc
    52861 agacgaacca agagaacatt tgcctttttt ctgcatgagg tgcttctgct aataattctt
    52921 tccataaata ataacagtcc tctatcagaa gaagggttcg ttcatcctca ggctcccaat
    52981 agaatgtttc gagagtagca atcacttcat tgatcaattc gaatgcggga agataggcat
    53041 tttgctgaat gagcgattca actgcttctt ggatgaactt tcgcatctcc atgatgaact
    53101 tcgctgtttt cctgtttttg tttttcttga taatatgatc gtatttatgg atgatagttg
    53161 ttaatttatc tgaataattt attgcatgaa atggcatctt aactttgaag cgttcgataa
    53221 attgcggatg ttctataaga atctctgtta aaagctcccg tacaagagag gaactagcgg
    53281 aggaaacaag ggacgagagt tcggaaagtg agtagttgga tttttttatt ggacgttctt
    53341 cgtaagcgaa taagacagca gccatgtgtt tgcatttttt tccttctatg gcatatagac
    53401 aatcacaagt catgttggtc ttttcatccc aagtgatcac taccttataa tcttccgtgc
    53461 cagaaactgt tgcggacaag tacttgcttg tatagttcaa atgacggaca aaaccgtgca
    53521 gatgatagct gtaaccgcgt tccaaaacgt tttcaggaaa atactcaagc caattattca
    53581 tctagttctc ctttc
//
LOCUS       DANKAY010000008        52805 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000008 DANKAY010000000
VERSION     DANKAY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(60..278)
                     /locus_tag="PWY98_000464"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="PWY98_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="PWY98_000465"
     CDS             complement(544..738)
                     /locus_tag="PWY98_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036245.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="PWY98_000466"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="PWY98_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="PWY98_000467"
     CDS             2090..3130
                     /locus_tag="PWY98_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2036246.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /locus_tag="PWY98_000468"
     CDS             3954..4286
                     /locus_tag="PWY98_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2036247.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="PWY98_000469"
     CDS             4905..5246
                     /locus_tag="PWY98_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2036248.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(5483..6733)
                     /locus_tag="PWY98_000470"
     CDS             complement(5483..6733)
                     /locus_tag="PWY98_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2036249.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            7143..7382
                     /locus_tag="PWY98_000471"
     CDS             7143..7382
                     /locus_tag="PWY98_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036250.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            7449..7658
                     /locus_tag="PWY98_000472"
     CDS             7449..7658
                     /locus_tag="PWY98_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036251.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            7718..8389
                     /locus_tag="PWY98_000473"
     CDS             7718..8389
                     /locus_tag="PWY98_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2036252.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8442..10418
                     /locus_tag="PWY98_000474"
     CDS             8442..10418
                     /locus_tag="PWY98_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2036253.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            10431..11186
                     /locus_tag="PWY98_000475"
     CDS             10431..11186
                     /locus_tag="PWY98_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2036254.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            11183..12007
                     /locus_tag="PWY98_000476"
     CDS             11183..12007
                     /locus_tag="PWY98_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036255.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            12308..14407
                     /locus_tag="PWY98_000477"
     CDS             12308..14407
                     /locus_tag="PWY98_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2036256.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(14445..16502)
                     /locus_tag="PWY98_000478"
     CDS             complement(14445..16502)
                     /locus_tag="PWY98_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDL2036257.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(16616..16828)
                     /locus_tag="PWY98_000479"
     CDS             complement(16616..16828)
                     /locus_tag="PWY98_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036258.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17015..17482
                     /locus_tag="PWY98_000480"
     CDS             17015..17482
                     /locus_tag="PWY98_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036259.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            17479..17721
                     /locus_tag="PWY98_000481"
     CDS             17479..17721
                     /locus_tag="PWY98_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2036260.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            17739..18041
                     /locus_tag="PWY98_000482"
     CDS             17739..18041
                     /locus_tag="PWY98_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036261.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18113..20167
                     /locus_tag="PWY98_000483"
     CDS             18113..20167
                     /locus_tag="PWY98_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036262.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            20167..22779
                     /locus_tag="PWY98_000484"
     CDS             20167..22779
                     /locus_tag="PWY98_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2036263.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            22776..25307
                     /locus_tag="PWY98_000485"
     CDS             22776..25307
                     /locus_tag="PWY98_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036264.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            25359..25691
                     /locus_tag="PWY98_000486"
     CDS             25359..25691
                     /locus_tag="PWY98_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036265.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            25692..26285
                     /locus_tag="PWY98_000487"
     CDS             25692..26285
                     /locus_tag="PWY98_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036266.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            26448..28142
                     /locus_tag="PWY98_000488"
     CDS             26448..28142
                     /locus_tag="PWY98_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2036267.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            28830..30707
                     /gene="ltrA"
                     /locus_tag="PWY98_000489"
     CDS             28830..30707
                     /gene="ltrA"
                     /locus_tag="PWY98_000489"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2036268.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            30838..31215
                     /locus_tag="PWY98_000490"
                     /pseudo
     CDS             30838..31215
                     /locus_tag="PWY98_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            31215..32420
                     /locus_tag="PWY98_000491"
     CDS             31215..32420
                     /locus_tag="PWY98_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2036269.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            32436..33080
                     /locus_tag="PWY98_000492"
     CDS             32436..33080
                     /locus_tag="PWY98_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036270.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            33091..33897
                     /locus_tag="PWY98_000493"
     CDS             33091..33897
                     /locus_tag="PWY98_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036271.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            33919..34149
                     /locus_tag="PWY98_000494"
     CDS             33919..34149
                     /locus_tag="PWY98_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036272.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            34695..34970
                     /locus_tag="PWY98_000495"
     CDS             34695..34970
                     /locus_tag="PWY98_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036273.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            35012..35434
                     /locus_tag="PWY98_000496"
     CDS             35012..35434
                     /locus_tag="PWY98_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036274.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            35453..37045
                     /locus_tag="PWY98_000497"
     CDS             35453..37045
                     /locus_tag="PWY98_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036275.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            37057..37392
                     /locus_tag="PWY98_000498"
     CDS             37057..37392
                     /locus_tag="PWY98_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036276.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            37539..37733
                     /locus_tag="PWY98_000499"
     CDS             37539..37733
                     /locus_tag="PWY98_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036277.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            37765..40137
                     /locus_tag="PWY98_000500"
     CDS             37765..40137
                     /locus_tag="PWY98_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036278.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            40198..41658
                     /locus_tag="PWY98_000501"
     CDS             40198..41658
                     /locus_tag="PWY98_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2036279.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            41669..43873
                     /gene="topB"
                     /locus_tag="PWY98_000502"
     CDS             41669..43873
                     /gene="topB"
                     /locus_tag="PWY98_000502"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2036280.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            43889..44038
                     /locus_tag="PWY98_000503"
     CDS             43889..44038
                     /locus_tag="PWY98_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036281.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            44112..44705
                     /locus_tag="PWY98_000504"
     CDS             44112..44705
                     /locus_tag="PWY98_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2036282.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            44721..45620
                     /locus_tag="PWY98_000505"
     CDS             44721..45620
                     /locus_tag="PWY98_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2036283.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            45623..46108
                     /gene="ssb"
                     /locus_tag="PWY98_000506"
     CDS             45623..46108
                     /gene="ssb"
                     /locus_tag="PWY98_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2036284.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            46474..46734
                     /locus_tag="PWY98_000507"
     CDS             46474..46734
                     /locus_tag="PWY98_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036285.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            47175..47381
                     /locus_tag="PWY98_000508"
     CDS             47175..47381
                     /locus_tag="PWY98_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2036286.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            47381..47632
                     /locus_tag="PWY98_000509"
     CDS             47381..47632
                     /locus_tag="PWY98_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036287.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            47647..48084
                     /locus_tag="PWY98_000510"
     CDS             47647..48084
                     /locus_tag="PWY98_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036288.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            48077..48784
                     /locus_tag="PWY98_000511"
     CDS             48077..48784
                     /locus_tag="PWY98_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036289.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            49165..49344
                     /locus_tag="PWY98_000512"
     CDS             49165..49344
                     /locus_tag="PWY98_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036290.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            49685..49948
                     /locus_tag="PWY98_000513"
     CDS             49685..49948
                     /locus_tag="PWY98_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2036291.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            49942..50292
                     /locus_tag="PWY98_000514"
     CDS             49942..50292
                     /locus_tag="PWY98_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2036292.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(50316..50930)
                     /locus_tag="PWY98_000515"
     CDS             complement(50316..50930)
                     /locus_tag="PWY98_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2036293.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            51244..51666
                     /locus_tag="PWY98_000516"
     CDS             51244..51666
                     /locus_tag="PWY98_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036294.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            51676..51879
                     /locus_tag="PWY98_000517"
     CDS             51676..51879
                     /locus_tag="PWY98_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036295.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            52090..52674
                     /locus_tag="PWY98_000518"
     CDS             52090..52674
                     /locus_tag="PWY98_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2036296.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        19228 a         8245 c        10507 g        14825 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattaata
     4861 taaagcatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
     5341 tatttagtct aatggtgata agtcaagatg aaatttaaga ttttctttat tgagtaatat
     5401 gttttgaagc gcacgaaact agacccgatc aaaatcaaat ttttttcggg ttgattcaag
     5461 gatttgcgtc aataacggat tgttatttct ctatcacact ccttggtggc gtatagagtt
     5521 ggtgtttacg tagtagcgta tccaccagac gcgtaaattt tcttgcggtt aggacgagtg
     5581 cacgtttgtg ttgatgcttt ggaacctcct tatatttact ttgataaaaa gctttgtatt
     5641 caggaagata attttttaca gagttggcag cttcaactaa gtagtatcta aaatatcgat
     5701 ttccttgttt tgtcagaggt gtattttgcg actgatagtt tccagattgg ttcactttcc
     5761 aacttaatcc agcgtatttt gccaattttg tctgatcttc aaatctttcg atttgaccaa
     5821 tttcagcgat taaaccggca gcatagactt taccaacgcc tggaatactg gttaagcatt
     5881 gatattctgg aatgacaaca attacttgtt cgatggcttt atctaattct ttgatatttt
     5941 tttcaagtgc tcgtatttct cgtacgagaa cacttaaaac aacattaatt gattcttgtg
     6001 caagagcacc taggcggtaa ctcatggtaa ttgcacgttg aattgcttta gcgatttttt
     6061 ctggttgctt gaaacggcct tttccttttt cttgaagtaa atcacagaaa gcttctaaag
     6121 gaagctcagc aagttcatcc aatgtgaaat cttccgtcat gagtgaaatg agggttgcac
     6181 taaaaagact cgtcgattcg tctcgcattt ctcgagcaag cgtattacat ttgtacgtta
     6241 gattttcaag aaaatgttgc tttgtacgaa tcaattgctt aataagttga tagcgagtac
     6301 gtgttaaacg ctgtaaggcc atgtatttct cttctttgat aggggaagtt gtgaatcgtt
     6361 gaatacgcaa gaaatcagcg attcttaatg catcgtttcg atcagtttta ttctcatcaa
     6421 acatgcgact aaattgtttc acccgaaaag gattttcaat cgttacaagt gtgttgagct
     6481 tctttagttt ctcatcttca tgaaaaaaca tggaaggatg gaagctgtac atggacgttg
     6541 actccatgcc aatcacaatt tgatcaaagt ggtagctgtc attgaattca agaattgttt
     6601 ctcgtatgaa tgaagcacct tcgatgtcat tggcaacaga gatttcagat aagatagaca
     6661 attgattgtc gtctgttaaa aaacaaacat ctagtttttc agagctaacg tcaataccga
     6721 caaataattt catggaagaa ggacctcctt tcgatataaa ttttggaaaa tgcttcgata
     6781 ctgtgggatt tcccagaaac tcactgtata gccacaacct cgcctaatag aatacggtga
     6841 taaaagagct agaagctgcc ttccaaacta ctatttgaaa gtctagtcca ttatcgaaac
     6901 agctaatgag ttggtagtga taacagtggt tcgggtaaca gactaaacag ggtagacaaa
     6961 cgctacagga ggaaatagca aattcccgtt ggatcctttc catacatcca ttgtactctg
     7021 agaaatcaca caatatcgaa acaaattccg ttaggaatgt gttattcaat aaattttata
     7081 atctagaaac attttgaata aaaactatta gacaaatgat attttacgag gaggaatagt
     7141 agatggcgcg accagtttta gaggcgatgg atatggatac tttgacaaaa ggaaggtatg
     7201 tacagctgtt agctcgtggt tattcaccta aagaagtatt caaagcctta tctaaaggaa
     7261 cagatatgga aaaagaaaga ttgcgaaaag aattcgatta ttggagaacc cataatggaa
     7321 tcaaggattt gaaaccagca cggccagtaa ttaaacgaaa gaaaaagata acccaagact
     7381 gaagtgtaga gcgtatgtga tcataggctt ttttattttt gcaaaaaaca aggaaggtag
     7441 gaaaactcat gaattatgaa gaaatcgaaa acagaaaaaa agtgtcgaaa gaaatggaag
     7501 aaaagttact aaaaacgatg aagcaaaaac accttaaaag attatctgtc gcgcaatata
     7561 tcaatgatat gcagttaaca ggaaaagaga aagcttgttt gttaggttca atgaaaaact
     7621 ttgaacagtt gagacgaact tatagaattc atttttaaaa ctttttctat ggaaatgaga
     7681 aaaagttttt ttgttttgtc tgaaaggagg cagatggatg aaaaaatggt tatttggttt
     7741 tttaggagtc actttaatcg tcttttgcag tgtgtttggc tatgttagct accaaaaaca
     7801 tgaaggagag gtgtttaagc aaaatataga aaaaaagatg cccgtagatc aaatcaatgc
     7861 acatgcaaag agttataagg aggatgcaac gaacgtcaat aatgatatgt ctttggggca
     7921 aatgttgtcc atacaaaaag aagcaatcga aatgggcgtc aataaacaag tatttgcaca
     7981 aattcagatt cctgcacttg gtttagcatt gccaattttt aaaggggcaa atcaatacac
     8041 tttgagtctt ggagcggcta cttattttta tgaagatgcc gaaatgggca aaggaaacta
     8101 tgtcctagca ggtcataaca tggaaatgcc aggtgtttta ttttctgata ttcaaaagtt
     8161 gtcattaggg gaggtgatgg acttagttag taacgatggc gtctatcgtt acaaggtaac
     8221 gcgtaaattt atcgtacctg ggtactttaa attaatcgat ggcgttcctg aagagaactc
     8281 tttcttgtct ttgccaaaga aaggagagaa acccttatta acgttgttta cttgtgttta
     8341 tacgtcacaa ggaaaagaaa gatacgtggt acagggagag ctacaataac aaggttttaa
     8401 tcaacgcgag tcgttgaaat atgaaatgag gagagtaaaa aatggcaata aatcggtatg
     8461 taaaaaaagc tggaatgctt gttggaatgt taggaatcat tgcaggctgc tttcaggggt
     8521 atcaagtaca tgcagaagat gtcacgcaaa aaacaccacc agagaaggtc aatatcacgg
     8581 ttcataaatt aatgtacgat caaggaacgc aattgaatgt tgatattgac gggatcaaaa
     8641 acgatggtta tacgcacgat caatatccta caggtgtaac gaaatacaac aaggcagatt
     8701 atggtgatgt tgagtttacg ttaggaaata ttacggatca agtattaccg agagaagatt
     8761 ctgatctaac caacgcgaaa gtggacgaga ttgtacaaga tgtagaagac aagggatcta
     8821 acagtgagta cgtcaagaat gcaaccaaca aaattacttc agctgtagat gaaaatggtg
     8881 agatcacttt tactgatcaa ccagcctatg tcaattccaa agggaatgta tatgtggttt
     8941 atgaaagtaa atccgctgct ggattagtca cacaaaaagc aaaaccaatg gttgtgattg
     9001 cgccaatgac agataaaaca ggatcaggat ttttaaaaaa tattcatttg tatcctaaga
     9061 acattgtttc taagttgagt tttgaattaa cgaaatttgg tgacgatggc gcagaccaaa
     9121 gcaaacaaac ccttttaaaa ggggcaaaat ttgagttata caagggcgaa cctggaaaag
     9181 gaacaaaatt gggtgatctt gtatcggatg atcaaggaaa gctgactgcg actgatttga
     9241 cgttaggaaa atactatttt gtggaagttc cttcggaagt tgtagtggga tcagatcaag
     9301 aaccaacagc agatcaatat ttattaggcg cagacgctcg caacgatgcg cacaataaat
     9361 taacctttga aattacgaat gatggcgtga caagtgactt aaaagcttca tacgtaaact
     9421 ataaagcacc agtgatcgat aaaacagtaa caaatggaac aggacaagaa cattctttcc
     9481 aaattggtga cgcagtaaat tatcaaggaa cgattcatat tccaacagac attgcaggtg
     9541 gcgcagacgg tatcacagta aacggtgtga aaagcgaaac ttctccttac agtgtattta
     9601 aatggggaga tacagcaggt aaaggacttt cttatgttgc tgaaaaagca gatattaaag
     9661 taacaaataa tgatggttca gtcgtattaa aagaaaatac tgactataaa atcgaaaatt
     9721 ctgatagtgg ttttgtgatc gattttatcg taaataatgg acaagtaagt gatacagttg
     9781 cgaatttaca tggtcaagat ctaaaaatga cttataacat gtttgtgaat gatagtgctg
     9841 aaatcgcaaa tccattaaca aatagcgtgg atttcgttta taacaataat ccatttaatc
     9901 aggaagaaca ccacgaaaaa acgaaagctg acgttgtgac ttatggcgca aaattcttaa
     9961 aagtcgatag cggcctattt ggaacaggta tcaaagcaac accgcttgaa ggcgcagaat
    10021 ttgcagctaa gaatgcagaa ggtaagtact acggtggact tactgataca gataaagatg
    10081 gtgtcaaaga agcagtttgg gtagacgatg tagcaaatgc agcaatccta aaatcagata
    10141 aagagggtca ttttgaaatc actggtttaa ctgaaggaga ttactcactg gaagaaacaa
    10201 aagctcctga aaactatcaa aaattaacaa aagagatctc tttcaaagta gacaaagatt
    10261 catacaaaga agaaaaccga atcacgatca aaaataatca aaaagcttcc gtaccaatga
    10321 ctggtagcaa cggtttccaa tcttacgtat tgatctcttg cttgttgcta ggagcagggg
    10381 gactttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta atgattattc
    10441 gtaagccccc aaaaagaaga gtcatgcaca aactgatgat atttttttgg ttgctgtcgg
    10501 cagtagcagt ctatttaatt ccagttgtgg aaacaaatgt agcgaaattt caacaacagc
    10561 aaagaatttt agccattgaa aatcttgaaa aaacagattc ttctttggat gttgcgaaaa
    10621 tagagttagg cgatcctgtt ggtgttctta caattccaag tatttcttta aaactaccaa
    10681 tttatgacgg aacttccgac aaaattttag agaatggtgt agggattacg gaaggaactg
    10741 gcgatattac aggaggaaac ggtaaaaatc ctttaatcgc ggggcacagt ggactttata
    10801 aagataatct tttcgatgat ttgccttcag taaaaaaagg gcaaaaattc tttattaagg
    10861 taaatgaaga acaacatgcc tatcaaattt atcgaattga agaagttaaa gcagaagaat
    10921 tacaaaaaaa ttatttagat tatcttatgc ctgaaaaaga ccaagatagg atcacgttaa
    10981 tgacatgcac accaaaaggt gtcaactcac accgcttttt agtgtatggt gaacgagttt
    11041 ccttcagtaa agaagattta aaactacaga aaaaagcaaa aaataggaaa tcaatttcat
    11101 tggattggcg attagcactg ctattcgctt ttttttgcgt tcctattatt tttatttata
    11161 aaaaaataag gaggagatcg gaatgaataa gagaaaatgg ctgtttgttt gtgtagcagc
    11221 tctcttttct actgtagctt attcgcaact gtctttcgct gacagcagtg atagtagtac
    11281 cactcaaaca atagataatt caattactgc accaagcgaa acggacagtt cggagaatac
    11341 tacagaagaa acggcagatt caaatataag ctttactgaa tcaacgaatc caacgtcaga
    11401 aagttcttca gagacttcag aaaagccgca agacgtagaa gatacgcaag tgatcaagca
    11461 agccattcat attcaaaaaa ttatttcttc gaatcaaatc aacaatcaag gcagtcaagt
    11521 agcccagcaa gaaggtatca atggcgcgaa gtttattgtc tacgatatca cgaatatctt
    11581 agaagaaatg tcacaagatg ataatgagaa aaatcaatct gtagaaataa ttgagtcaaa
    11641 attgaaagat cgagcaaaaa aactttcata cgaccagttg aaaaaagtgg ctgaaggaga
    11701 aaccaaaacg attgatgatc aggaaggcgt gattgaattt tccattgaag tacctgcaaa
    11761 caaaaaacaa gcatactata tcgttaatga atcaagccct gaaaacattt caaacagtga
    11821 agatattgtt ttattgacac ctgtttcaaa tgaaaaaggt gaatttttga cggatgtttg
    11881 gttataccct aaatcagaga aatcagagcc gaaagaagaa attaaaaaag ttgtgtcaac
    11941 tggtgtaaag aagaactttt ttgaaaattg ttgggaattt tttgctcaat tattttttga
    12001 taattaggat gaaatgatga aaataataag aatattagtt tttaatctat cctttttagc
    12061 cgcaacaata ttgtgttgcg gtttaatttt ttctcaatta tcaatttatc caagatatca
    12121 acgattgttc attcattttc taaacattca atgtgcttcc atgccattag aggtgattaa
    12181 aaagctcttg atcaatccaa tttttatggt tagtttggtt cttcttacag gattggtaac
    12241 gtgggtatta ggaaaaaaag aatgaaaagc ttacgatcgt ttaaaaaaat attagatgga
    12301 gggcattatg gaaaataaaa ttcattgcac tacgggaaga gtaattactt tagtgttgtt
    12361 tttgttcctt cttctttttg cgaggggaac tgcagtccag gccttagaaa aagttgatcc
    12421 gagtcaaatc aaagaaaata ataatctggt gctttatcat gatagtgatt cattgagaaa
    12481 tatcgaagta actgggatca atggatttaa gatactagat gagtattcaa ctaaaaaaca
    12541 aaaagtactt gcttggccaa aagtcaaaga tttgaaaagt acgtcaaaaa tcgttgccca
    12601 ttatcaaaaa gtaggtactt atctaggaaa agaaattgat tgtgacgtga ctttttctaa
    12661 ttttgtagcg cacgctgggg ttaaccaaga cggattagct aaaacaatta atggcaaaaa
    12721 gtattataat tatttgctct ttgataaaaa tatttatcga ggatttttca tgcagtcaat
    12781 caatgtcatg aatgcaaaac tagaatttaa gtatgcagga acaaaaacaa aagtagcttt
    12841 gagtgaacca cagccagaaa ttcaatcaaa aatgagatcg atgagagcag ctggtattga
    12901 tttaaacgat acatttctat ccatttacag tttaaatgga cagtatcagc aacgtgtgtc
    12961 ttggtataat gacaataatg aatctgtcgg tgaacgtaat attgatatga gagaatttgt
    13021 tgggtatgaa aaaatgggta gcttacctta ttttgtcaca acagatacag catgtgcaga
    13081 atacaaagct cctgcgttat caacaaacaa tttaacttca aaagtagtgg gaggacgtgc
    13141 agaaaaggct tatagctcga atgatcattt caccgatgtt gtaggagctg atacttatca
    13201 cagaagtggt gtaacgtata cgcttcaagg cgcttcccca acattcatga ttggcgcaaa
    13261 tacgaatagt atgatgttta gctttgatac tgcattgcta tggacaccac aaccatcgaa
    13321 gcctacaaaa gaagtgttta acaaagctaa tactgaagag gcagcacaca atattgacaa
    13381 aaaagtgatt ccacaaggat cagatgttta ctatcatatt caccaaaagt ttgatgcatt
    13441 aacagtcaac acaatgaaca aatacaaatc atttaaaatc actgatacct ttgacagcaa
    13501 aaattttgat atggtatcgg atgggaaaaa ctatgatggc gcattgtggt atgttgaagc
    13561 gaatggtact tggaaaaaag ctgatacagg aggaaagtta actgtatcag gtaatacagt
    13621 cacttatact gcttctgaca gctttttagc taacgctgca aattacggta gggattatat
    13681 tttgcagctc catatgagag caaaaaatga tgcaaaagga attgcaccaa atgtggctac
    13741 tgtaacgttt aacaatgatc cgaacaaagg aacacaaaaa acaaatacag tagaaaacta
    13801 tttctctctt aaaccaaata aaaaagtgga acaagatggg aaagatgttg atggtcgaaa
    13861 tgatggtaaa aaaggaacgc ctacagctcc tttaaatgca ggatctgaag tacaatatct
    13921 agtcacacaa aaatggcata caaaaggtgt agacattgcc tcagatcatt acaagcaatt
    13981 tagtattcaa gatccagtgg aagatcgttt gacgtataaa gaaggttctg cgcgagtgat
    14041 agatatgagt acaggaaaag atattacttc agaaggtaca ctaacctatg atagcaattc
    14101 aagaacgctg aagtgggaag cttccgctga tttcttaagt aaaaatcttt tagatggacg
    14161 agaaattcag ctgatattta cagctaaaac tcctttgaat gaagaattgg atatcgacaa
    14221 ccaagcaaaa gtttcagtag aaagtattga gaaagcaact aatgtagtaa cgattggggt
    14281 aaaacctaac ttacctcaga tcattgtgcc aaaaactgga tcgaatcatt tagtgctaac
    14341 tgttattgga agtatattat ttcttacttt agcatcattg ggctatgtag ttttaaaact
    14401 caaataaata taaaaacaag gctgagaatc agccttgttt tcttttattt gaagattgga
    14461 ttcgcactaa taaatacatt atctacgcca attctaccag cagagtcaac tacaaaatta
    14521 tgacctaatc cgagaatact ttggttatta taaagtaaat ttgcattatg ggcagggcta
    14581 tttttccatc ctaccatggc atcgatagca ccatcgtctg ataaaaggtc atcaatatac
    14641 agcccagttt gacttatgtt ttctccctta gcaccgtagc agcctaagct ttccaataaa
    14701 tcttggaaag taccaccacc agggcgatag tgactgaagc tgacttgtaa ttctttagct
    14761 cgaatatctg cagcctgttg aacaatatca ttgaattgca ttggggcaac accattttgt
    14821 tggcgataat catttaacca agcaagttct ttttgacgca taaagttacg taattcgtct
    14881 aatgaaatct ttttatattt gaatgtgatt gttttatttt gatcgacgtt attaatggat
    14941 cgttggccgt ttttatccac ggtaacggta ggatcgtcat ttctgcacaa gaaataattt
    15001 tgatcagtca tgtttttcca aggagtagca aagctatccc cttcgtatac tgtttttgat
    15061 tcactagaga gaacagcgtt ggtatcagca tttacatgat taacagtcac attaaattta
    15121 cgagtgtagt tgaaaatata attttgactt tcagtcacat tgtttaaggt tactgaattt
    15181 gttggttcta cccgatcgtc taaaacatat ccttgaatag aaggagcagt taaagtgaca
    15241 ctgtcaccgt gagtgacatt gactatctgt tctttcaaaa tattaactcg tccttcgttt
    15301 ttgtagttgt tattgcacaa caaattgatt tgatgcactt gattataatt gaatgtgatt
    15361 gttgtattac ctgtgattgt tttgctttga gtatcattac caatcacttt gtagctaaaa
    15421 ttatctttag cgccttcgct atatttaaat gcttttgcct tagcagtgta agtagaacca
    15481 gaagttactt ttgcttgatc tgtagcaatc acttgacctg tttgattgtc aatatggtta
    15541 atagtaaccg tatattcttt tggtgtttca ggtttgtgtt gcaacccttt aaatgcagca
    15601 tcaagactat ttttagcatt gtttacttgt gtttgagaaa ccttagaatc gtctagtact
    15661 tttttagcat tggataaagc agtctgaaaa ttattccaag attcttctgt aaaatctcct
    15721 ttagcagttg attttactcg gttgtataaa gcttgtaatt cagctttatt cacagtagga
    15781 actggttttt tctttaatcc tttgtaagca gaatctaatt tatttttggc actatccact
    15841 tgcttttgag tcgcttttga gtcatttaac actttcttag cattgtttaa agcggtttgg
    15901 aatgcgttcc aggtattgtc ggtatagtca ccttttttcg ttcccttaac gctcgtataa
    15961 agagcttcta gagccgattt attcactttg ggtttagagg tgtcagcgcc ataccaaccg
    16021 ataccttcgt atttccagcc atagtctttg gttaaccatt gtacttcttt ccaacttgca
    16081 gtgtagtgat gtgagccaga ttttacacca ggaaggaaaa ggcggtagat cggtttatta
    16141 tttttttcgg cagaacgcca ggcaggccct tcataagtcc agccgtaatt tttagtcaac
    16201 catttcactt catcccaact agctgtgtaa tggtgatcac gaagaccttt attgtacaaa
    16261 cgatagacaa gatggccacc gctagtcggt gcttcccagg cggggccttc ataatttccc
    16321 cattttatct ttactagatg gtcacgttct tttttgctag aggtgtaaaa atgttcatta
    16381 ttatttggat tgtaaaggcg atacatgacg ttagttgaag cagcgtcaac agatccagca
    16441 agactaagtg aggcagcaaa tgttccaata gctaaaacag tttttggatt aaataatttc
    16501 attttttcaa ctcctaaatt taattaaaaa tgtttacatg attattatat atcggctaac
    16561 cgatatagtc aataaaaaat aagaaaagcc ttatataatt ttggcttttc ttattttatt
    16621 tgttagttga agtattaata tcaacgattt cttcagccaa tttatttaat ttcatagcat
    16681 ttatgaatct catgttgtct atactagagc gtttgttggc aaggtcactt actgtagtta
    16741 agggtacgcc agattctatg gatatagaac gaactgattt accactatca aacagcaacc
    16801 attcgatttt ctttttatca gcgtacattt caatgctcct tcttgttttt tctttattat
    16861 agctcttttt tgtaatgaat cacaatatca agagaaaaat tttctataga aaggtggatt
    16921 tttatgagta ttcgttctcc gtgtatccaa atgaggtttg ttgctacttt cgttatttga
    16981 tatactaaaa ataaacaacg aaagaaggaa taaaatgaag aaaaagcaac tatggtttgt
    17041 ctctatgttt gcaatatttt ttattatttt aagtggatgt tcaagtaata agaaatcgga
    17101 agctaaagaa gataatagtt tattgcaacc aggaactgtt tggaaagaag aagtaggtgg
    17161 tcttattcat aatttaaaaa ttatagatga tactacctgg gaatattctg aaagtgtatg
    17221 gcatccagat cctgtacaaa taacagttaa gaggcaaaaa gactatgaag gtttagaaag
    17281 atacaaaata gtagattctg caggcattag ggaatttata aacaaacctg attcattatt
    17341 tattgttgtt ccctatgaga aggatggcgt aaaaaaaata atttttcttg gatctagcaa
    17401 ggatgagaag cgaacgaaag agaaattaat tcatgatgga tcagagttaa ataaatacaa
    17461 attacaaaag actagtgagt gattgctagt ctttttattt atcctgaagg aggaatcaca
    17521 gtggcaaaag ttaaaacacc attcgaaaaa gccgcgtcta aaatcttaga acgccgtggt
    17581 gtttctgaaa tacagtggaa aaaagaagtt cttgaaaaag cacaatttga cctattacga
    17641 ggaaaagata aggacttaga agaatatatc tatcaacgtg aacgtgaaaa acttgtattc
    17701 gatgaaatta ttaaacatta aaaggagcga ataatcttat gtttgaatca attacaatga
    17761 attttttaac tcaaccgaaa gtagtagctg cagttgatat ttttagagca ttacaaactg
    17821 ccgcaactag tttgaaaaat aatctgatga tttttgcggc tgtagctgcc gttgcctcca
    17881 ttgctcttgg aggtattttc ttcttctttg ggcgaagagg agctgaaaac gggaagacac
    17941 acgtaggtta tgtattgttg ggggtaggag ttattttagc tgccgcttct attgtagttt
    18001 caatgttctc aatgtttgga caaaccccac aaggactata aaaaaaacaa gtaggaggag
    18061 taaaaagtac gtggctactt tttactcctt tttttatgaa aggagaatat tcatgttttt
    18121 tacgatttca ggaaatgcaa aattggataa agagttaata agaaaagcaa cgaagaaagt
    18181 gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg aaacggaaga
    18241 acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg ttaaattgac
    18301 tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat acgtttttgg
    18361 cccactggat tatcaaattt cagaaggttt ttatcctaca ttgatagatt taatccgcct
    18421 tgatgtgttt aaagatacgg atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa
    18481 actgctacaa gttttagaag aagagttaga gagcgaccaa agaaaagtga atcaagtgag
    18541 tgaagtcgaa gtaaatgaag aagtaccatg ggaacgacca atctttccaa agaaggagtc
    18601 aaaagaaaaa gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat
    18661 atttgaggaa gagaacgatg tgatgggtca attggaggta gatccctttg aagaaacaat
    18721 ggaggatgaa gagacggacg aagtagaagt tgatccagtt gaagctaatg aagagtcaac
    18781 atggactgaa ggggattttc aggaatatat tgaaccactt atcagtgaag aagtagaaaa
    18841 aaacgaaaca gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc
    18901 agagcatgaa tggatgaaag aaaaaaacaa attattttta aatgagcttt attcgaaata
    18961 tcaattaccc caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg
    19021 taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc atgagcttgc
    19081 acaagaagat ttattagcca cctttgaaga acgtcaacaa gaaacagatc aagaaattaa
    19141 tgagtatgcg acggaacagt cgtctaattg ggagaaagta aaagaggaac taaataaaga
    19201 agaacagcga gtgattgaag aagaagtaca aaaagtcaaa gagcggtacg aaaagaaacg
    19261 cgacaatgct tattccttgt gtgtagaaaa gatcaacaat tttactaaga cgaatcaaga
    19321 agcgttatcg aaagaaaaag aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga
    19381 gttctaccgc ggtttacgta cagataagct aaagattagc aaagaattaa atgaacaact
    19441 gaataagcta tatcaaacaa cgactgaagt tcttatggaa aaacacgaac agatccagca
    19501 agaattacaa gtacaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt
    19561 gaaagaacaa gaagaaaaac aacggcaaaa agaaaaagag cgtgaacaat tagaaataag
    19621 aaaacgggaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac ggttagagca
    19681 agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg
    19741 tcaagcacag ttgaattatc tagcacaacc tcaaccagta gctgctgttc aaacgccagt
    19801 gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat ggatggtggg
    19861 atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt ttaatcatat
    19921 caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg aagaagccca
    19981 ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc gtaccaatca
    20041 gtcaacaaca gagtcaagca tggaaaatag caaagagagt gatcctagta aggctaaaga
    20101 aggatcaagc gattcaactg tacactcaaa cacttccgaa agttcagaat caaatgaggt
    20161 gaaataatgc gtattagaga aacaacgaga cgaaaagaca aacgattcaa tttagaaaag
    20221 aataggtatc tttctcattt ctatcgctat actccttcag gagaagagct accagaaaaa
    20281 catgacaaac gtatttctca aaagattttt tatagtggct tagcttatct tcctttaaac
    20341 tatgttgtta caagaatacc agaagcattt catcgatttg agattattcc aggaagttta
    20401 caaggagtga atatttttca gttacaagaa atgccgcagt tattccattt tgattcaact
    20461 ggtttttgga atcctttgac cccaacaaca agtttaggca cacgtttagt tgtttctacg
    20521 atttgtgttg ttccagctgt acttatggga ttgaaaatgg acatgtggtt tcgtccgcta
    20581 gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca acagatttat
    20641 ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc aatcacacat
    20701 tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt tggtacctct
    20761 cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc tcgtgcagaa
    20821 aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg tagtaaagat
    20881 gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc actgcaatca
    20941 atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga catagatact
    21001 gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa cgcttcagat
    21061 aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt tgcgttgtta
    21121 gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct cgttgagctt
    21181 ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt caaacagttg
    21241 aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa aaaacaatat
    21301 ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac ggcaatcacg
    21361 ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc aacgattgat
    21421 tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag tcttttaaat
    21481 caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga acgtgtcaaa
    21541 gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc aggtgatcaa
    21601 gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga attttctcaa
    21661 agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg agaaacccat
    21721 ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag taacttatta
    21781 gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat gcttacacct
    21841 gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt atataaagaa
    21901 ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat tcattttatt
    21961 ttagatgaat ttgggaatat gccaccaatg gataatatgg aaggaatcat gacggtcaca
    22021 gctggccgaa acatgttgtg ggatctgttt attcaatctt atcagcaact ttatgcgaag
    22081 tatggagaac aaaacgcagc gattatcaaa gaaaattgtc agacacatat ttatattatg
    22141 tcgacagatg ataataccat cgaagaattt tctaaaaaag tggggaataa gacggttgaa
    22201 caagagaaca gtaccatcaa ttcattaggt atgaatactc atatcaatcg caatgttgat
    22261 tcagatcgta tcttaactcc tgaaagaatt tctacttttt tagaaggaga aacgattgta
    22321 ctcgctcctt taaaacgaca agatttgaaa ggtcataaaa tacggccgta tcctattttt
    22381 aatcatgggg aaacgaatct tccttatgct tatcaatata ttgaagattt caatccatcg
    22441 tgggatatca atgatataga catacattgt acacatgcgg acttggatct taaggcttta
    22501 caaattgatt atgattattt cctaccagat gaatatgcta gaaatctcta tcatggactg
    22561 catgatcaat tagaagaaaa aaacgaagaa aatatgtttt ataaaatcgt tcaatctttt
    22621 gagaatgaga cggctaaaaa attgttaatt caattttacg aagaacaagc agcagatcga
    22681 cttcaagaat atgttgaaaa gttgatcaaa aataaagaaa tttctgaaat gattgggaaa
    22741 cggttgttat tggaatctga aaaggtggtg aaagaatgat cattggagga attttttctg
    22801 gcatagccga ctggtggtca ggcttattcg gagaagggga tgcaaacccc aaaaccgtaa
    22861 cgaaagtctt acaagattat gggaattatc tgcagtatca agatgtattt agttggattt
    22921 ggaacacttt tctgtgggga ttgattaaag gattatataa tctcaatact ttactagaaa
    22981 aaaccattta tcaaagcttt gatttaaaag acttattgaa cgccgctggg gtaaatgcac
    23041 tatttcaaag tttgattagt aaggtattgg ctcttttttg tgtcgtcaca ttaatttatt
    23101 taggcttgaa attttttatg agtaaacacc caccaaaagt caaaaatatc ttggttaatt
    23161 tatttatggc catgatcttg atttttggtg gtagttcttt aatcgatgaa gggttaaata
    23221 tcagtaaaag cttttacgga gacgtcactg cagcaaataa agcagataaa acgggatcac
    23281 ctgcttttca aattattcaa aataatgtga tcgatgtcag tacgttacta gcaactgacc
    23341 cgaataaagt agaatcccta ccaacagata aaagaaatgc tttaacagcg aaaaatttta
    23401 aaacagcgaa tatcaatggg attattactc ctgaacaagc taaaggtatg gcaggtaatg
    23461 ataaaagtgg gttatcagat atacaaaagg atcggttaaa ggcattacaa taccgattgg
    23521 aactagatga taatgggaaa gaaattcctg ttcaaattag tgacgaaggc ttagcaaaat
    23581 atgtctatac ttcagggtat cgtcgataca gtagtaaacc aggtattatt ttagctggtg
    23641 aatcttcttt ggcagtagcc tatttattta tcctatttac gatcgttact tgcattattg
    23701 agctggtatt taaaaaattc tatttagtga ttgccgcgtc aacggacttt gagacaggtc
    23761 aacggatgaa aagtgcgata gaagatattt ctcaatcctt cttgttattg gcgtttacga
    23821 ttttagaatt acgaatctat accttaatgc tttcaggtat cggtgatttg catgcggcag
    23881 gaaagatcaa tggattcttg tatgtcgttg ctttgattgt actaaccatt gcgctattca
    23941 aaggctctca atcagttacg aaaattttcg gcgttgatac aagcctgaaa aacggcagta
    24001 attctcttct tagcatgttt gccttaggaa atattgcgaa aaatgtgggt agcggagcta
    24061 aaaaccttgc aagtgctggt aagagtggtt taagccattt aaatgatatt cgtaaaaatg
    24121 gatttggcca tcgaaaagat gttacccctg ataacgggga agaacaatca gacgataaag
    24181 ctacaactgt acctaatagt aagaaaacag tttccgaaat gttcgggaaa gcgaaaaatg
    24241 gtgtttctaa agccgcagaa ggtcttggtt acgtcaatga acgtgggtta aaaggaacag
    24301 ctgaagatct tacagaaaaa gccaaagaca ccttagaaga taaattagac ggcacgagag
    24361 ctaaagaatt ggctgaagct gtgaagaatc catccgaagc actagaaaaa caaaaagaaa
    24421 aagcttcaga agtcaaagaa ggactaaaag atgcgtatgg tgaaggacaa gtcaatgcag
    24481 cagtgaaagc taatacaaca accgaaaaag aaaatcaaga aggtatagtg attccttcag
    24541 tcaaagatca ccaagaagcg ttgcactcta atgaaaattc gattgaagag aaaaattcca
    24601 tagaagaaag aaattcagaa gaaggaaaaa atggacagtt accaaaaact gatccagaac
    24661 aatatcgcta taaacctaac agtgaccttg aaaatgagaa gcgaatttca gatttatcaa
    24721 aagtaaatca agaagacaaa gcagaaaatg agggacagtt accaaaaact gatccagaac
    24781 aatatcggta tagatctgtt aactccgaaa atgaaaaacg catttcagat ttaccaaaac
    24841 gaaatcaaga agtacaaacg gatgaggatt ccatcgttct gccaaatgat aatggaatcg
    24901 aagttggaac tgaaaaatca acgaaaattg gtttacctaa ggcacaaaat ttaccaatga
    24961 aagatagtcg tttgaatcaa ttcaaggata atgatcaaag ttttgttcat caatcacctg
    25021 caggtactgt tcaaatgaaa ccaagtcgtt tgcctgaaaa tggaaatctg acaactgttt
    25081 cttacaattt atctgaaagt ggtgagacag ggaatgcgtc cgttcaaaat gttgtgacaa
    25141 gagaagctgc accaaaacct attcaaaacg ttcatacagg ttacaagcca gcaaatgtgg
    25201 catttactgc accaacaaaa ataaatgtga caagagaaac aaaaaacagg aacaatgcat
    25261 tgaaagaaca actattgagg gatatcaaac aggatatcaa accataaaag gcctacaagt
    25321 taggcctttt ttctatgtat gaaaaggaga aatcatttat ggataaatat agtaacatga
    25381 aaaaccctcc aagatttagt ggagaatgga agtttttcgc ttttttcttt ttagatctat
    25441 taacgcttgg tggctgcttt ttttgggcga aaatggtcaa taatcttgtc cacttaccac
    25501 tcttttttgc actcttgaat tatggcttat ggattgcgat agcgttagtg attgtttggc
    25561 gaccctataa taacccaggt caacgacaaa tttttgcgat tatcgagtct attttttttg
    25621 cggacaagaa ccattatcat gcgttagatc ctaaccgaaa ttatcatggt acgtataagg
    25681 aggatgaata aatgcgagtc aaaggacaaa attcagttga aaggaaatcc attttgcagt
    25741 ttggaaaacc agaagagcgc tctatcttgg acttatttcc atataaatcc tgtacagaag
    25801 agggagtctt aattactaga gaagatcatt ttcaacgtta ttatcgtgta gtttctacag
    25861 acgtggaagg attaaatgaa caagagaaac tagaacgaat gaatcagttg accaccgtca
    25921 tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg gatctaacgg
    25981 aacaaatcgt gcaaaagcgg caattattag ataaaaatcg tatggaacag tccttaggaa
    26041 gaaatctaca acgattaaaa aaatatgaaa agaaattagt cgaagaaatc caggaattga
    26101 aacgagcaga acaagaacgt cctgacttaa gcttcttttt cgtcattgaa ggaaagacaa
    26161 tcaaggattt acaaaccaga aaccgtcagt taatgcgatc tagtggcatt ttagggttaa
    26221 aaccactcta caaaaaagaa ttgatcaaga ttctgtatcg aatgaacaat atgaatgatg
    26281 agtaggaatg gagaagagag aatgaaatat ccgttgatat ggttagaaaa agatgttccg
    26341 aaggaagaga ccactagaaa atttcctcac actttaagcc tgggtaagtt gggaaaaggg
    26401 caatctttta accaagtaaa gaatcaaaca agaagaggtg actgtacatg ttagaagcaa
    26461 ttaaaacagc tttttcacca caaaatgatc aaaaagtact tgatccatta aagcaactag
    26521 gaaatcggga aatagaaatt gtaaaatcca aagggtatga tttagatttt ttaaaacaag
    26581 tgatgccaca aggtgggtta aattttgatg aagaatatgt gacgtatggt aatggttatt
    26641 cacgatgtgt gacagtttat cactacacaa aaaatcctac gcttttttgg ttggccaatc
    26701 tcatgaacaa tcctcataca gtggccacac tcgatgttag cacagatgaa aagcaaaaag
    26761 tgattgatca aatcaatcgt gccttagatg aattaaatga tcgatcagat aatgaacgaa
    26821 aagcgactga ccgaaataaa agcgcggaag aatacctgga actagcggaa tatgctagaa
    26881 aattgacttc taatggggaa atttctaagc aagtcaaagt gagagtacat gtttacggaa
    26941 acacgattca agaagtagat gaacgagcgc aggaactgat cgaaaatctc aaagggatgg
    27001 attatcgtgc ggtgacttat ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca
    27061 tgttcgatga acaagctgaa tggttcggaa atatcaaagg gcaatcacta ccatcgttaa
    27121 acattggcgg aggtttcccg ttccaccacc aaacgctaaa agatccagga gggacacaat
    27181 atggggaaac catgacaggc ggcccatttg tctggaatgc gacgtataaa aatagtgtac
    27241 ggttgtctac taataccttg cttctaggaa tgatgggagc tggtaaatcg accgttctta
    27301 aaatgatcgc ggaagcccat ttagctgcag gagactttat ttggggcttt gaaaagggaa
    27361 aagactttat tcctttctta aaggaatata acggtattat ggtgcgttta gacggatctg
    27421 acggaatgat caatccttta gagatatttg ccacacggac gtatgatgaa gcatccagtt
    27481 tatatgacga tggatcagtc aaagatttga agatcaatga agcagcgtcc tatcaaacac
    27541 acttagacaa agtggtctat caggtacagt tagtgagtcc tcagctaaaa ggaacgatga
    27601 aagcagaatt taagacctac ttaaatcgtt tttatgaaga gtatggaaca gtaccacgcg
    27661 gctttacttc ctcaaattcg cgttcaaata ctgatacgca agtcacaggg aaagatccag
    27721 aagcttatcc gacgttcaaa gaatttttag actttttagg acaactggaa ttaccaggtg
    27781 cttcccaaga gaaaaagaat cgaaaagaag aaatggaatc catcgtagaa tccttatgtg
    27841 agacgtatgg catgattttt gatggtcata gtacgattcg acaccttgac caacaacagt
    27901 tggtcttttt tgacattgac tctattacag gcttagatcg aggggtacaa caatgtcaaa
    27961 tgtacatggc tatgacgttg atttggaatc aggcattgat tcaaggaaga agacaacgac
    28021 gtttaattaa agaaaaacaa atggaagaac aagacttcaa acgcttcttg gcgatgattg
    28081 atgagtgcca taatggtgcg acctgttctg cagtgaaaag ctgtgggttg caagtagtct
    28141 aaaaactatt gtaataactc ttataacgag aagtcaaatg agtgggtaac gccacaaagg
    28201 gcttccactg atactccgac atgcaacaag tagtttatgc agaccaacgt tgtgtgaagc
    28261 tcggtgaagt cggctgaagg ataccgtaag gtaaaagaaa tagtgaatac cacgaaagtt
    28321 ctctagaggt aaagggtgta tccgataagt cgggtgtcca aaaaaactta atatggtgag
    28381 tatgtacaaa cgtactgacg aactagcgaa tgtactgtcc gaaaggcata gctcctagaa
    28441 atgggagaga cagtgagcaa aaaatcactc attgtgctaa cttgtggttg agtaaaagtc
    28501 gttgttatga aagaccatgc tgcgttacag gcgtaggaga agttaggggg caaaagctag
    28561 catctaagtt aaggcatgtg gatagatata agggaacagg ggaagccatc aacatggaga
    28621 gtgcatactc gatgaagtgg tagtaagaaa tatgtgaatg acaaataact tactttagtg
    28681 atggtgaaag tagagtcacg actgatgaag gttctgtaat ggaactggag gaacaggctc
    28741 gattcgtctg taaaagtcat gaaacccgtt caaccatggg attctcgtat gactaaaaag
    28801 gtcacgagcc aattggagcg tgataaacca tgacaaaaga aacaagtcca aaagatagga
    28861 agttgcggca tgcagaatat tatggcatga cggaaatctt cgataagata tactccaaaa
    28921 gtttgaaggg agataatttc aagaatctaa tgcagattat catttctgat aacaacattg
    28981 tcctagcata tcgtaatatc aagagaaatc acggtagtgt gacaagagga gaagatggag
    29041 taacgattaa agatattgaa aatctcaacc gtaatgaatt cgtagaaatt gttaggaagc
    29101 gattcgcaca ttataaccct cggaaagtaa ggcgtgtaga aattcctaaa tataatggta
    29161 aattaagacc gttgggtatt ccatcaatat gggatagaat cgcgcagcaa tgcatcttac
    29221 aagtattaga accaatatgt gaagcaaaat tcaataatca cagctatggt ttccgcccaa
    29281 atagatcagc agaacacgca atagcagatt gtatggcccg aatgaatcaa agtcacatgg
    29341 aatatgtggt agatgtagat atcaaaggtt tcttcgacga agtcaatcat actaaactca
    29401 tgagacaaat ctggtctatg ggtatacgag acaaacagct tctcttaatc attaggaaaa
    29461 tgttaagagc acccgtagta cttccaaatg ggaagttgat atacccaaca aaaggaacac
    29521 cacaaggcgg aatacttagc ccattacttg cgaacatcaa tctcaacgaa tttgactggt
    29581 ggatagcaaa tcagtgggag gaaaaagatg ccaaagagct aaaacatcat tttaggaaaa
    29641 atggagcttg ggataaaggt aatatctatc acaagctcag aaagagtaca actctaaaag
    29701 aaatatacct agtgagatac gcagatgatt ttaaaatttt ctgtagaaat tataatgaag
    29761 cgaaaaaagt tttctatgca actaaaatgt ggttacagga gcgtctaaag ttaccaatat
    29821 ctgaagaaaa gtcaaaaatc acaaatcttt caaaagaaga aagtgaattt ttaggcttta
    29881 cgatgaaatt agtgaccaaa ggtaacaaaa aggttgttaa tacgcatata ggtacaaaag
    29941 cacaaagcat gattaaagtg aaactaaaaa aacaaatcaa acgaattcaa aaaagtccta
    30001 atagcaaaga tacctttatc tatattggag tctataacag tatggttatt gggattcata
    30061 attacttcaa aattgcgact cattgtagtg tagcaatgcg aaaaattgca agagaactca
    30121 gaccaagttt tgaaatacga ctacgagaac aaggaatctc aaaagaagga caatacggaa
    30181 agtacgaagg atacaagcct tacctacggt caggacaaat acgatatata agagggtttc
    30241 caatattacc agtagggtat attcaacaca gacatccgat gtgtaaaaaa agaaatatta
    30301 ataaatacac cgaagaaggt agggaagcca ttcataagaa gcaacaaacc gtagaagaat
    30361 ggaagatatc gtggttgtgt agcaatccag tgcttagtaa gagagcaacg attgaatttg
    30421 cggacaatcg aatttcaaaa ttcatcgctc aaaagggaag atgtgcagta acaggtgagg
    30481 agcttatctt atctgaaatg cattgtcatc atattatccc ttatcatgaa agcaaaagtg
    30541 atagctatga aaacctagtg attgttactg aggaggttca tcgggtaata cacgctacac
    30601 aaagtgaaac gattgaggaa cttctaaaat atctaaaatt aaatccaaaa caaaaagaaa
    30661 aactcaatga gttgagatta aaagttggta acgaagaaat atcttaataa atatttgtag
    30721 gacgggaaaa cagaatgtct tttatagacg agggaacgcc gtatgcggtg aaagtcgcac
    30781 gtacggtgtg gactggggga aaagacggag ataatatcaa actcttacct atcagtatcg
    30841 attaatccag attttattga aacggtaaat tatgtcacaa actttcaacg agaaatgcgt
    30901 aaagtagaag caatgactgt tttagctaca caatccccac aagaaatggt gccagaaaat
    30961 atgtcttctg ataacttctc gaaactgaag aaagtctttg aattttcttc tacgaagatc
    31021 tttatgcgta tggatgaatc tattttacgt catatagaag gattagtggg taaatcaatg
    31081 acgaactcag aattagaatc cattcctcaa caaaatcaag gagacgctgt gatcaatttt
    31141 ggctcaaaag aaaccattcg tgttcatttt agtccgaaca aacgtcaatt aaaattgttt
    31201 gatggaggaa aataatggaa aaccaaaatg aaagtttaat caagcagtat gttaagcgtc
    31261 gtgcaaagcg gcgcttattt ttgtggttgt ttggtacttc tgcaggtttg attactattt
    31321 tgatcactgt gttcgtgacc ctatttctaa tacttgcggc gggctcgatt gataattcgg
    31381 attcagattc aagcagtggt ggcgaagctt ttacaggaga atattcggaa ggcttaccga
    31441 tttataaaga aatcaaagga agaggccctt tttctgatga gattgcgcaa tatgccgtag
    31501 gtgcagcagt gaaatataaa ttattaccaa gtgtgatcct ttcgcaatac ggttacgaat
    31561 cagcgtttgg tacatcggcg tccgcaagaa atgatctcaa ctattttggg attacttggt
    31621 ttgatggttg tctgtttcct aaaggaactg cacgaggaat tggtggcatt gaaggtggct
    31681 ggtacatgaa gtttcccaac tcaaaagctg cattttccta ttacggcttt atggtagcca
    31741 cacaatccaa ttttaatgct tgtgtaggaa ataaaagccc tggcgcaagc ttattgatct
    31801 taggacgagg cggttatgcc gctgcaggaa ttacggaaga tagtccgtat tatacaggtt
    31861 gtatgtcaat tatcacttct aataaattaa ccgaatatga tgaattcgcc atcaaacatt
    31921 ggggtgaagg tggcaataac aacggcacga ttactggtga atggacgaat ccattccctg
    31981 gcagcagttt agataaaagt tccttcagcg gtggtcaatt atttggaacg aatccaggcg
    32041 gtgaattccg tccgaacggc ttccatgatg gattagattt tggttcagtt gatcatccag
    32101 gaagtgagat tcatgcggtt catggcggaa aagtcgtgta cgttggcaat cctggtattt
    32161 caggtttggg tgcgtgtgtg atcgtgatta attacgatgg cttgaatatg gtgtatcaag
    32221 aatttgcaaa tagtacagga aattctcgag tgaaagtagg cgatcaagtg aaagttggtc
    32281 aagtaattgg gatacgagat acagcacact tgcatttagg atttactcga atggattggc
    32341 gtcaagcaca aggacatgca ttcattgatg atggtacatg gatcgatccg ttaccatttt
    32401 tgaatagcag taaaaaatag atcaggagaa aaaatatgga aaataaaacg ttaaaaatcg
    32461 tcttaacagc tgcaattagc gttagtttaa tcggcaatat tgcgttgttt tggcagctga
    32521 aagaggaaca agcagcaaat acctataaaa ttgaagaagt ggctaaaaac gcgtctgaga
    32581 agctcaaaaa aaattcctct gaggcacagg cgaagatcga caagttaaat caaaccatca
    32641 atgagaaaaa ccaagaaatc gatcagctga agcaacaaga tggcacaaaa gcaaaagagg
    32701 aagtaactga agcacaaaga aagacggcag aagagttcgg tcaattggcc attgataaat
    32761 cattaagcag aaatgaatta acggaaaaac taaaagatgt cgcaacccaa gaagtgatcg
    32821 acaaattaag tcctacagac gatgatcacc aacatgatga ttacggaagt tactccttta
    32881 cattaggtga cgtgcaaact tacgctcaaa caagcagtgt aaaagaggaa caaaattttg
    32941 tcgtatttgt tgattataca atcggcaatc cgcaattcaa agatgtcaaa ccacaaaaaa
    33001 taaaaggtgg attaactgtc actgaaaaac aagtagatgg tcaatggaaa gtcatagact
    33061 ttagttattt cactcgttag gaggaagaaa atgagtaaga aaaaaggaga aagacaacgt
    33121 ttcaaaaaac gaaaaagtta tcttagatgg ctgggaatct ttatgattct cggctttttg
    33181 attcttgcta gtccgattta tacgaatcag aaggtagata taccagatga ggtaagcacg
    33241 aacgcctttt taaacacgaa agagctgaca atggtttcaa aagaaaaagg agcgaaccag
    33301 caattagttt ttgaatttgc gatcgatgat gaagatcaaa gcttgttaaa agaactggct
    33361 aatcttaatt atcgcgtaga agtaaagaca gctaaagggg attatcaagc aatacgagcc
    33421 aaagtgatta aggtgtctga tgattatttt gcagtgaagc taacgcatgt accagaagaa
    33481 tatatcgcca tgcgtttaac cattattcct gaaaaaattg atccaaaagt ggatatgcaa
    33541 gaaccacaag atctgatttt ttatattcac gaagataagg tcaaagatca cgtgaaagac
    33601 gaagattatg aacaacatgc gatcaattac aaagtgaaag gatacaagaa agaacaaaaa
    33661 gcagctcaaa aacaaatgga atctttccaa gcaaccattg atttgaatga agaattagtg
    33721 aagaagctaa aagatcaatt gccttatcaa gtcgcagatg atcaggaaaa tactgaaaac
    33781 aagattaata gctaccagca agaaattgaa acatcgaaaa cgcaactgaa agatcaagaa
    33841 gagatcattc aaaaacttca agaaaaaatt gatcgtatga caaaagaaaa gatttagaag
    33901 gacaggtgga gtgaaaaaat gaagcgaaca aacttggctg acaaagatac tttcgctgag
    33961 cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat atgtcgagaa
    34021 gtgaatttat ctaacacaac aatatctgag ttgaagaatg gtcgaaagaa tttagatacc
    34081 ttaaaattca gatcatttga agctctatat gtagctgcta aaaaatattt tacagctgat
    34141 aacaaataaa aaacaggagt attttatgtg aaaaaagtat ataaaaaatg cttatgaatc
    34201 agctatatca ccttaaccat ttgttaatga gatgggagaa aacattaacc acgattctaa
    34261 cgcaaacatc aagcacataa aacatttaac actcctcctt tctggctgaa gatttcaacc
    34321 aaacaggaag acagcgtatc aattatagca tacacttttt cttaaattca tttttcatat
    34381 gacaaaatat ttccttctaa tcgacaaagt tagaaggatt ttttattgtc taaatacaca
    34441 aataacctta atttttaaag agcataagga aacgtgatat cactttctca cgaaaggtgt
    34501 taggtaggct gtgcctacca cgtttctaag aaagataaat gatatcaaaa ttttggattt
    34561 gtgtgtagat ttgtgggtag gatgaaagcg aatgtgggca gtaaaataag cgtttgtgtg
    34621 tagcaaaatc aaaaaatgtg tagggatatg tgtagctaca catatcccta cacataataa
    34681 atttggagga aaatatggac ttattaatta gaaatattga agaaaagtat attaataaaa
    34741 ttgacaaacg ttgtgaagaa cttacggtca agactggcaa aaaatggagt cgaaatcaat
    34801 acctaaaagt tttgatcgaa aatgattttg atcacgctct tctgaattat aaaaaagacc
    34861 aatttgatcg gttgttagaa aaatttgtgg atattcaaac ttataatacg aaaactttag
    34921 aagaatatat tgcaacaaat aatcaattaa ttggtttgtt aattgaataa taaaaatagt
    34981 attgtgaatt tataaaagga ggaaatcaat tttggcaaaa gcaacaagaa aacatgtata
    35041 tctttacgat atgcatatcg aagtacttga tgaaataatt tctactcatg aagatatatc
    35101 taattatagt gaagcagtca gacatttgtg tatgatgtat gatccaaaaa tcgaagaaaa
    35161 aataaatcca attcaaatta aattgaatgc aatgggaaaa gaaatttcta ttttaaccga
    35221 aatggtttca gagtttgcgg atcttcattt gaaagataca ggaatactaa ttggtacaaa
    35281 ttcaactgtt tatcaagaag cagaagaacg tgtggaagga aaaattaaat ccaatcaaac
    35341 caaaaaatat tcttctaagt taaagaaacc agtgaatgaa tctgttcata ccagagtaga
    35401 tccatttgat ccaacgaaga ttctaaaaag ataaaagtag aaggagaacg tgatgaataa
    35461 atcgcctgca gttattctta cttgtcaatt taccttgccc aataaaaaat ctttttcaac
    35521 atacattgat tatatgacaa gagaaaaagc actggaagaa atggaacgac gtacgcccga
    35581 agaagaacag gaactgcaac gaatcaagaa tgctttagag gatttttata ttcctgaagg
    35641 agaaactttt tcggaaagta agacggataa agaattcagt gaaacgtcga atgaagctca
    35701 atcgttgatg gaacatggaa tcaattttga caagttagag gaacaagatt ttaccaaata
    35761 tctttcttat atgacccgtc actatgcgtt agaacaaaag aaagaactga cgcaaaggga
    35821 acaaaaagaa taccaacggt tgaaccaagc aatgaaaaaa tatcaaacag agccacataa
    35881 aaaagcttca ggaaatcttc ctggagtttt ttctatggct tcagacgaag tgaaaggaag
    35941 tgatctgaag gagatcaaac aaatcttcca aagaggacaa caaaaaggct caatcattta
    36001 tcaagatgtt atctctcacg acaattcgta tcttgaaaaa atgggcttgt acgatcccaa
    36061 aacagatacc ttagatgaag aaggtttgaa agcagctgga agaaaaatga tggagacatt
    36121 attcgagaaa gaacaattga atgaaacagg ttactggatg gcaacgattc accgtaacac
    36181 taagcatatc catattcatt ttgcagttgt tgaaaaagaa aatacgcgta aaccgtatac
    36241 tgagattgaa aatggtgtga gttatatcgt accgaagggg aagagaagac aagcaacatt
    36301 agacgcaatg aaaaccaatt atgtacatga gttagaacgt ttctcttatg aaaaggatcg
    36361 aatgatcggt atggagaaac gaaatttatt gacacgaaaa agtgatttgc gtaacacctt
    36421 aacaaaagta gtaaaagaac cagcacgtta cgatcgacac gccatgaact tattaaatga
    36481 agtatataaa accctacctg aaaaacgttc ggaatggaat tatggagatg aaaatcgaac
    36541 caagctttca ccatttacaa ggggaaaact tgatgagtta accaaatatg ttttaacaaa
    36601 agaacctgga tatcaagaat atatacagat ttcaaaagca ttgaaagaag aagccaaaag
    36661 cctttatggg gaaagcaaaa gagaaacaaa gaatgcagaa aaaaacgccc tctttgattt
    36721 aaaaaagcgc acaggaaatg cgattttaac agaactgaag agacaggata gtcaaataaa
    36781 accattaata gaaagtgtca atcagatgca atctatttac aatgaacgat cctttcgaac
    36841 actcaaacag ttgccaacaa atgaatacaa gtatagttat caagaattcg taaagcgtag
    36901 gaaacaattc caacaaagag ttgtcacgca gcgaagcttg cgtcaattaa atcgaacaat
    36961 taatgcacat gaagaaaaat accacgcaag taaagcatat gaagagcaac agcgaagaat
    37021 ccaaatggaa caagaaagac aataggagga agagaaatga aacaatattt agatcaatgg
    37081 aaagtcatag aaggatcact aaaagaagaa cgaatcgaac aacttcctga ttgtttagaa
    37141 aaagagcatt tgtttcagat aagcgaaatg ttgagaaacg aacagtttga tccaaatcaa
    37201 tttttagttg ttgagtaccc agctactggt gtgtattgtt gtaatcatgt gaagggtgaa
    37261 aaatatttta ttattcaaga atacgaaggt aagttagcgc cttattatac tacttgggaa
    37321 ataaatgaag agggaatcaa taatttccca tgtgaaagca ttgaagagtc tatttctctc
    37381 acggaatgtt aaataaatag agacgtaaaa gagtcctgaa cgtgattgtc caggactctt
    37441 tttcaatgaa ttgttttccg ccaattcact gagtggtgcg attcaatttt attatatcaa
    37501 aagaaagtga gattccaaag tgaagggagg aagaagaaat gactcagaca acgattggtg
    37561 ttgtggttga aaaaagcagg gataatctcg tttttgtgac agaaattcaa acaggtagag
    37621 cttttgtagt cacagacaaa gctgcaaaag cgtatcaaaa tggcaatatt ttaacgttaa
    37681 atctgactac gaagatgttc gttgatgcag tagaaaatta tccatttgtt tagacaaacg
    37741 atattttaca aggaggaaac ggacatggca gaagaaaaag aaccactaga taatagtcgg
    37801 ttgggaaagt ccaaaagaaa attagtacgg ttgcaaaacg aattaaataa tcaatttcaa
    37861 aagctatttg atcaccaaaa attgacaaat gggcaaccaa tgaatgacaa acgaaatggt
    37921 cgttcatggt ttaaccagca agaaaaaata gaaaataaaa tacaaagttt gagagaagag
    37981 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
    38041 ggctataaca agtacggcgg actggatatg acgatcgata atattccaag aatcaaagaa
    38101 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ccgccacgat ccgaaaatat
    38161 caacggaagt tggaaacatt agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat
    38221 atcctccctg aagtacaagc gatcattgac agcggacgag tgacgcaatg gaaaaaaaat
    38281 ccaaccattt attttttgaa aggctatcga aaagttgctt tagaactgaa tgaaaacggg
    38341 ggatttgagg aatcaaaaaa ataccaagcc acgtcggaag aggaaaaagc agctgttcgt
    38401 gaattacttg aagaggctgc accaactgat tctctgaagg agaaagaaaa agatcatttc
    38461 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggggag
    38521 atcgtaacat tagaattgat cgatcaactt aaaacgttgg ataaccaata cgctgcagaa
    38581 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt agatcagatc
    38641 cgaatggatc taggggatgg cgtcaacagt aatcacccca tctatttaga gttagcaaaa
    38701 ttgtgcgaac aagtacctga gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg
    38761 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
    38821 gcagaagaga tcaaacaaga agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa
    38881 aagcataccc attcttctga agacgtcaaa gaacttttga actttatgtc tcgttttcca
    38941 gaacgatcct ttagaaatca attattgatt gaggaacaat ttccaggtgc aacggcttgt
    39001 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
    39061 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
    39121 gaaacagaag ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
    39181 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca gttaaaacct
    39241 gaagactatc cgaaaatctt tccgaaccgt gtctttgatt ttcaattgga taaaaaagga
    39301 cagtcagaac ttcaagctgg gattcaagcg gtagccaaca atattggaat acaaattcga
    39361 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt
    39421 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
    39481 catttagcta caagtattca tgaattagcc catgcaagaa tgcacaaatt ttcagaatta
    39541 gataccgcaa ctaaagaatt gcaggcagaa atgacttctt atattgtgtg caaacatttt
    39601 ggaatggaca cttcggagaa agctattcct tatattgcag catggacgaa gaatggtcag
    39661 acgttggatg ataaggaagc ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc
    39721 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
    39781 gagatccaac cagcagaagt gaagcaggag cataaaaata ctgtagaaac ggcagagaat
    39841 aagaatattg tgcccgttgg ttcactttgg atcgacaaga aagacgggaa agtaatggaa
    39901 gaagatacgg gtcgaacgtt acatttgaag gatgcggcgt ttattgaaga tccgcagcat
    39961 aatcaagtga ttttaaaagg aaaaagttat ggaaaagaga ttcaaacggc gttaccttca
    40021 gaagaatttg cgctaggaaa tccattccga ttttcacttg aaacagtgtc agaagtggcc
    40081 aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac aaaaagaacg cgtataaaac
    40141 aagtaaaaaa gactatctga aaaacggata gtcttttttc ttttgggagg tttgaaaatg
    40201 accgaaaaaa aaatgagtct tattgatcga tgtaaacaga tcgatatagt ggattttgct
    40261 cgaaacaatg gaatggctgt agtcaataaa ggacgagact atcgcttaga agaccatgat
    40321 tcatttgttt ttgatcgaag aaaacaacgt ttttattgga acagtcaaaa tattagtggc
    40381 gacattattg aattagctaa actttttttc atagacaaag agatccaaga ctctaagcaa
    40441 caatttaaag cagccactga ttttattcta aagaatgaag acaaaacaga acgagtggaa
    40501 aatcttcatt ttgaaacaga aaagtacaaa gatcaccccg ttgattatca accactcaca
    40561 gaaaaaggaa ggaattatct gaaggaagaa cgaaaactac cagagtggct gattgattat
    40621 gcagaaaaag aagggttgat tgccgaacta aagccaaaat atgaacggca aaatttctta
    40681 gttcgagacg atcgtttaga tcatgcggtc gcctttttat ggaaagatcc acagacaaaa
    40741 gagacagtag gggcgagtta tcaaggaacg ttcattgatt atgaacgttt tggtgagaga
    40801 ggcacatata agcatataga taaaaattcg acagcgaatc atggctttaa cctaaagatc
    40861 ggtgacccta agcagttaaa attttttgag agtagtattg atttacttag ctatgctgca
    40921 ctgaatcgtg atcagttaaa cgatacgtgg ttagtctcta tggaagggtt aaaacaccat
    40981 gtgatcagtc attattttgg tgaggcagta tctgaattga gaaagaaaca agcctttcct
    41041 caatccatcg agatttgtgt ggataatgac cgtgcaggac atattttcta cgaaaaagaa
    41101 caattaatgg gcgcagtcga tccatttacg aatcagaaag tacgttgtga acgaggaatc
    41161 gctaatgatt ggcaagtgcc aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa
    41221 gaaaaggtca caccagaagc tattatggcc atacacaaga cagaaaacaa tctgcaatta
    41281 acgaaccaac tggtttccgc tcacaaagta aatgcgtttt ttggccagca gctttctgtt
    41341 aacgattcaa tagaagccat taatttgaaa gatatttgta gagaagtcgc aaaagaatta
    41401 aaagtttgtg aacgcgtgga tggtacgtac gattttgatc gtttttatca agaaaaaggg
    41461 gatattaatg cgcagattct tttctcttat aaagcagaac aatactacaa aggctataaa
    41521 aatcatgaac atgaatttat acctgaagta aaaaaggact ggaacgatca attgaagcat
    41581 gagatccagc aacaagaaat cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga
    41641 caacaagaaa gggagtgaaa tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc
    41701 acaagcgaaa gcttatgccg atagcttttc taaagctacc cgaaaagatg ggtattttga
    41761 gatccaagat cgattgttta caggagaaac tgtgatcacg tatggttttg gtcatttagt
    41821 cgagttagat tcgcccgata tgtacgatga aaaatggaaa caatgggcat tagaacattt
    41881 gcctattttc cccaatcaat accactatca tgttcctaaa gataagaaaa agcagtttag
    41941 tgtcgtcaaa cgacagctgc agtcagctga tacgattgtg atcgcgactg atagcgatcg
    42001 tgaaggggag ctgattgcct ggtcaatcat tcaacaagca ggcgccaatc aaggaaaaac
    42061 ctttaaaaga ctttggatca actccttaga aaaagaagcc atttatcagg gattccaaca
    42121 gttacgagac gcagaagaga cctatccaaa atttgaagaa gtacaagcac gtcagatcgc
    42181 cgattggtta attgggatga acggtagccc actgtatagt ttactcttgc agcaaaaagg
    42241 cataccagga agtttttctt taggtagagt ccaaacccca acactctata tgatttatca
    42301 attgcaggaa aagattagaa acttccaaaa agaaccatat tttgaaggaa aagcccaagt
    42361 aatcgctcaa aatggcgcat ttgatgcgaa gcttgatcca aatgaaaccc aagcgacaca
    42421 agaagcattt gaagcctatt taaaagaaaa aggtgttcaa ttaggaaaac agccaggtac
    42481 gatccttcaa gtcgaaacag agaaaaaaag tgcggctagc ccacgtcttt tttctttatc
    42541 gagccttcag tctaaaatga atcaactcat gaaagctagt gccaaagata ctttggaagc
    42601 catgcaaggg ttgtatgaag gaaaatatct aagctatccg cgaacggata ccccttatat
    42661 tacggaaggg gaatatgcgt atctgttgga tcacttagac gaatacaagc attttttaaa
    42721 agccgaagcg attccgactc ctattcatac accgaatagt cggtatgtga ataataagaa
    42781 agtccaggaa cattacgcga tcattcctac aaaaacggtt atgacagcag cggcttttga
    42841 acaactaagt ccactgcagc aggctattta tgaacaagtg ttaaaaacaa cggtggccat
    42901 gtttgcggaa aagtatacct atgaagaaac aacgatcttg acgcaagttc agcagcttca
    42961 gttaaaggca atcgaaaagg tgccgctaga tctaggttgg aagaagttat ttggaaaaga
    43021 gagcgacgga aaagagaagg aagaagagcc gctacttcct aaagtgacaa aaggagaaat
    43081 ggtgacggtg gatctacaag ttcttgaaaa agaaaccaag ccaccacagc cttataccga
    43141 aggtacgttg atcacggcga tgaaaacagc tgggaaaacc gttgatagtg aagaagcgca
    43201 atccatattg aaagaagtgg aaggaatcgg tacagaagcg acacgggcga atattattga
    43261 aaccttgaaa caaaaggaat atatcaaggt agagaaaaat aagctcgtgg tgacgaataa
    43321 agggattttg ctttgccaag ccgtggaaaa agagccgctg cttacgagtg ccgaaatgac
    43381 cgccaaatgg gaaagctact tgttaaagat cggtgaacga aaaggaacac aaaccacttt
    43441 tctagccaat atccaaaaat ttgtctctca cttattggaa gtcgtaccag ggcaaatcca
    43501 atccactgat tttggatcga cgttacaaga agtgaaagca gccagtgaaa agcaagaagc
    43561 cgcgcgtcat ttaggaattt gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa
    43621 ggtggcagca tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa
    43681 gaaacttaca gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt
    43741 gaaagggtta aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt
    43801 cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg
    43861 cactacaaaa taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat
    43921 cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag
    43981 actgcagcaa ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat
    44041 tcctttcttg ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta
    44101 ggggaagagt aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat
    44161 ccttgaagga tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa
    44221 aggataaaat agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg
    44281 aattgtatct cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg
    44341 atctttttca agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat
    44401 acaatgtatc aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag
    44461 aaaattttga cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct
    44521 atgatttaga gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg
    44581 taaaacaaga agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa
    44641 gaagatacat ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt
    44701 attgagagga gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg
    44761 cagcagagaa acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat
    44821 tagaatgtat tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga
    44881 ttggatcaaa atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa
    44941 attcgaaact aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta
    45001 caaaagatgg aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt
    45061 atccaggttt tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat
    45121 tagatattac ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa
    45181 tgtttgaaga taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa
    45241 aattacatgc cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc
    45301 tgtatatgat tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag
    45361 gaacattcaa ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc
    45421 ctcaattggt gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact
    45481 attttgccaa agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat
    45541 caattgtaga gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga
    45601 atcaagaaat ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa
    45661 agatccagat ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt
    45721 taatcgtaat ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat
    45781 ttggcgcaaa ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt
    45841 tgtcggacga attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac
    45901 agaagttgtg gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat
    45961 gcacgctgat caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc
    46021 gacgcctaac aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga
    46081 tattagcgat gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg
    46141 tttatgttat aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct
    46201 ggcgggctag tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt
    46261 ctttcttggc aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca
    46321 tgattgcaaa aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga
    46381 ttaggccggg gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg
    46441 gatttttgct ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat
    46501 aaaatccgag taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat
    46561 gaatcgatta attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat
    46621 gatggaaaat acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt
    46681 gctagcatgt atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa
    46741 gaagagttgt agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa
    46801 agaccttttt aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac
    46861 cgccaccgcc gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc
    46921 ttgcaaagca agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcgt
    46981 tatctcttta ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca
    47041 aggacaaggg taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgatttatt
    47101 ctggccaaca aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg
    47161 aggcagaatc aaacatgcga aagaaatttg attattgggg tgttcccttc agcgaattat
    47221 tcccaaacta tcatgcacca catactgttg aatgcgattg tggagaaaga gcgaaatgta
    47281 tcaaaagcta tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg
    47341 gcgattacat tttgattgat gagaaaggaa agaaacgctg atgaaaacac aacacaaatt
    47401 attgactgta aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt
    47461 agcagaagct ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc
    47521 accgactatg ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga
    47581 aaacatggaa acttatgaaa tcgaatattg gttaaccaaa ttagagaact aaaaaggaga
    47641 aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca
    47701 caaaaaatgg aggatatgac gttcctatac catgaaacac gtgtacccaa aaaacattac
    47761 aaggaaaaat tgtcagtcgc agtggaagaa atgattgaat caggtgtcga aatgaatctc
    47821 attgcgacgt actatcgaac actggaagaa ctgaaaaaac aaaatggaaa atggtttttc
    47881 caagccttac tatgcttgga agtaggtgtc aaaccaagca ctatcaaacc ttccgaatat
    47941 caagccttag aactgactta tgcaaaattc gcacagatta agaaagctaa agcaatggct
    48001 agcgactggc tagatacttt tgaaagtatc gaaaaaaatg gtgcatttta tacgattgaa
    48061 tcggaggaga aagataatga ataaggtggt ttatcagcta acaacagaag atgtttcgca
    48121 atttttaaca agcaaagaac acttgaatga agtaatcaga cggattcaaa aagactttag
    48181 ttgtatggaa gaaatgaagt actttattca aagagtggaa gaacagttgt atatggaaag
    48241 cgaaacaagt caaacgaagg tcacagtcag ccataatcat ttagacgtgt ataaacgtcg
    48301 tcctgaaacg gtaaaaaaag gaacagagct tctacgacta gaaacagaaa aatacatcat
    48361 gaatgtgtac ctagaaagga gtgagctggt cgaaaacttt gtcagctaca cgatttgtag
    48421 gaaacactat tactcagaat cagaatatta ttcaattttc gagagcatgg aattaatgga
    48481 agaagaaatg gaagagctaa acagcaaaaa tgcgacggta gacgattatt accttgtatt
    48541 tactaggttt ttgacgtgtg ggacggaatc tatcgtgtta atgggaatgg attatgaact
    48601 cgctgtaccc gaagcatttt tgagggaata tgtagaagaa gtagataacc caggcgacga
    48661 ctggtacacg actttaactg attttttaga cacctacact tacgacgata cagaagaaat
    48721 ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga tttgaaagga aaaaaggtaa
    48781 ataaccattt ataccgaaaa gtgaggatgt gtgaaataag aacaaaaaac cttgaaatta
    48841 agggaatggt tcttatttcg caccaatcgc tccaaccgcc cccgccgctt aggtggtgcg
    48901 aaataagcgg gcaaagcccg ataacctaag agaaagaagg agatccatat gaataaaatc
    48961 attcctctat tgttcgtact gtttgttggc gagatactta tgcgtcatgc tgcagctgta
    49021 catttaaact tcgtagtatt cttaggaata ctggtatttt gtttgatctt ttggcgattt
    49081 gtattagccc ttgtgagtct actattcaag atgattgcag ccatattttt catagggtta
    49141 tttcgatgaa aggagagagt actaatgcca attgcaatag gaaataaacg gttacccgtg
    49201 acattagatg aaaagagaca aaaagaattg cagcaactaa agcagaagta cggaaaaagt
    49261 gaatctaaga ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt
    49321 gatataccag cactaaaaaa gtgacgtcac ttttcatttg aagaaacaaa agtgatagca
    49381 cttaattgtt cctactattt agaatatttt ctatgtcctt tttattccat tttgtatcaa
    49441 taactatgaa atctttaaag tatccagaag agattatttt tagatcaatt tttttcttac
    49501 ctgattctaa agattttgtt ttcttaaaca tattatgatt tcctttcgac tattttcata
    49561 taaatgttaa catagtttat ggattcatgg aatgttaatc taatttaata gaaaaggata
    49621 attaagtata tacgtttttt cttatgtatg ttatactatg tatatacaga aagaggtgtc
    49681 tactatggaa atgttaccta taaaaaaatg gggaaatagt aatggcctta gattgccaaa
    49741 gtatattatg gaatatcttg gaattcatac agaagacaaa gtgaagataa tacaagaaga
    49801 agtaaacggt cagaagcggc taattatcga agcggctaat atagaaaatg aattaacaat
    49861 tgaacagtta tttgaaaatt acactgatga aagaaatcat gtgacgattc aaaatttagg
    49921 tgaagcagta ggtaatgaaa aatggtgaga aaacctagac aaggcgatat tctattattg
    49981 aatacagcac ctcgatctgg acacgaacag acaggtaaaa gaccttatat tgttttaagt
    50041 catgatatta ttgcggatta tagtaacgtt gttacagttg ctccaatttc tagtacaact
    50101 agaaactatc cgctatatgt aaatataaac tcagaatatg aaatgaagac aacaggaaaa
    50161 gtattattag atcaactaac gacaattgat tatgaagcca gagagtgtat ttttttggaa
    50221 aaagcgcatg atagtttagt tgaggagtta ttgatgaaag ttagaacagt ttttcaaaaa
    50281 gtaagcaaat aaaatagaaa cagcagcatg atcaactatt cgtgattgac catgctgtct
    50341 ttttttattc tatatacggt ttgtctcgta acattgtagt cttttgcaat cttagcgatc
    50401 gcaacacctt tttttagatc ctcaacgatg tttttataaa caagacgacg ttggggatct
    50461 tttgcattgg cactgtatag cttaggacgt cctttataaa caccgttcgc ttttgcaatc
    50521 ttaatgcctt gtgcctgacg tcgtttagat tcggtacgtt cttgttcagc gatcatggcc
    50581 aaaatttgaa tgatcaaatc tttaatgaat ttatccagga gaggattgcc aattgcttcg
    50641 gccatgatcg gtagacttgt gatgatcagc tgcacatttt tcttcttcaa gtaattgact
    50701 gaagcgatga tctcgtcgta atttcttcca agtcgatcaa tagcttccac aataaaaata
    50761 tcttgatctc ggacgaaatc cagtgcttct tgaaaaacaa gtcgatgagt aatcgttgcg
    50821 ccagattgtt tctcaatgaa aattctctct gcaccgaatt ttttcatttc ttcaatctgc
    50881 cgttgttcat tttgatcgat ggatgagaca cgtatgtatg caactctcaa aatttttacc
    50941 tactttctgc aactatttaa aaaacaccct aatcatacac gagaaaacca gatttttcaa
    51001 tgtcgcggtc gtgtctagtt agggtatacc ttatatagac aaaaactaat gatggaggaa
    51061 atagtatgtg tcacttttct ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa
    51121 gaaaatttaa acgattatga cattaaaaat atattttatg aattttctag tatataactt
    51181 gatttatctg ttaagattta ttaagaaaac gttttcttat ttttgatttc gaggtgatag
    51241 acaatgaaaa ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact
    51301 ggaactacaa ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat
    51361 aaccaagaat taactccttt agataggaac ggaatagcca ttggtaaccc aactaacgac
    51421 ttaactgtta ctgaaaatta tttaatagtt tctccattac ttgctcgagc agcttctaaa
    51481 acgatacgag tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct
    51541 caatcacgga attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta
    51601 caaagttata aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga
    51661 aagtagggat tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat
    51721 attctaagta aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta
    51781 caggagacac aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga
    51841 aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc
    51901 tagtcgacat tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact
    51961 ttcttttttt gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca
    52021 atatctatgt tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga
    52081 gtgatttttt tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta
    52141 caagaagaca gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg
    52201 gcaaaaagta atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca
    52261 cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc
    52321 aatcgactga atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac
    52381 aaatcaagtt ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag
    52441 cgtaatttga tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg
    52501 tttggtggtc gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg
    52561 gatagcggta cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt
    52621 tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg
    52681 tcatttaatg ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt
    52741 tttgtaagtg tctactgata acttattctc aaatgttgta aggattttat tcatttgtta
    52801 atatt
//
LOCUS       DANKAY010000009        51363 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000009 DANKAY010000000
VERSION     DANKAY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1002
                     /locus_tag="PWY98_000519"
     CDS             <1..1002
                     /locus_tag="PWY98_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2036297.1"
                     /translation="ITSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1006..2040
                     /locus_tag="PWY98_000520"
     CDS             1006..2040
                     /locus_tag="PWY98_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2036298.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2142..2858)
                     /locus_tag="PWY98_000521"
     CDS             complement(2142..2858)
                     /locus_tag="PWY98_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2036299.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2827..3612)
                     /locus_tag="PWY98_000522"
     CDS             complement(2827..3612)
                     /locus_tag="PWY98_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2036300.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3603..4352)
                     /locus_tag="PWY98_000523"
     CDS             complement(3603..4352)
                     /locus_tag="PWY98_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2036301.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4650..5174
                     /locus_tag="PWY98_000524"
     CDS             4650..5174
                     /locus_tag="PWY98_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2036302.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5190..6689
                     /locus_tag="PWY98_000525"
     CDS             5190..6689
                     /locus_tag="PWY98_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2036303.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6777..7379)
                     /locus_tag="PWY98_000526"
     CDS             complement(6777..7379)
                     /locus_tag="PWY98_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2036304.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7483..8439
                     /locus_tag="PWY98_000527"
     CDS             7483..8439
                     /locus_tag="PWY98_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2036305.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8744..9700)
                     /locus_tag="PWY98_000528"
     CDS             complement(8744..9700)
                     /locus_tag="PWY98_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2036306.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9874..10434
                     /locus_tag="PWY98_000529"
     CDS             9874..10434
                     /locus_tag="PWY98_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036307.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10427..11251
                     /locus_tag="PWY98_000530"
     CDS             10427..11251
                     /locus_tag="PWY98_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2036308.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11261..11878
                     /locus_tag="PWY98_000531"
     CDS             11261..11878
                     /locus_tag="PWY98_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2036309.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11896..12579
                     /locus_tag="PWY98_000532"
     CDS             11896..12579
                     /locus_tag="PWY98_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2036310.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13262..14545
                     /locus_tag="PWY98_000533"
     CDS             13262..14545
                     /locus_tag="PWY98_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2036311.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14561..15448
                     /locus_tag="PWY98_000534"
     CDS             14561..15448
                     /locus_tag="PWY98_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2036312.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15465..16301
                     /locus_tag="PWY98_000535"
     CDS             15465..16301
                     /locus_tag="PWY98_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2036313.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16304..16927
                     /locus_tag="PWY98_000536"
     CDS             16304..16927
                     /locus_tag="PWY98_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036314.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16924..18216
                     /locus_tag="PWY98_000537"
     CDS             16924..18216
                     /locus_tag="PWY98_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2036315.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18575..18672
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18788..19369
                     /locus_tag="PWY98_000538"
     CDS             18788..19369
                     /locus_tag="PWY98_000538"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2036316.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19372..20679
                     /locus_tag="PWY98_000539"
     CDS             19372..20679
                     /locus_tag="PWY98_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2036317.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21095..21583
                     /gene="purE"
                     /locus_tag="PWY98_000540"
     CDS             21095..21583
                     /gene="purE"
                     /locus_tag="PWY98_000540"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2036318.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21576..22700
                     /gene="purK"
                     /locus_tag="PWY98_000541"
     CDS             21576..22700
                     /gene="purK"
                     /locus_tag="PWY98_000541"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2036319.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22713..24008
                     /gene="purB"
                     /locus_tag="PWY98_000542"
     CDS             22713..24008
                     /gene="purB"
                     /locus_tag="PWY98_000542"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2036320.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24130..25518)
                     /gene="ltrA"
                     /locus_tag="PWY98_000543"
     CDS             complement(24130..25518)
                     /gene="ltrA"
                     /locus_tag="PWY98_000543"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2036321.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26087..26464)
                     /locus_tag="PWY98_000544"
     CDS             complement(26087..26464)
                     /locus_tag="PWY98_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036322.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26720..27448
                     /locus_tag="PWY98_000545"
     CDS             26720..27448
                     /locus_tag="PWY98_000545"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2036323.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27448..27702
                     /gene="purS"
                     /locus_tag="PWY98_000546"
     CDS             27448..27702
                     /gene="purS"
                     /locus_tag="PWY98_000546"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2036324.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27704..28375
                     /gene="purQ"
                     /locus_tag="PWY98_000547"
     CDS             27704..28375
                     /gene="purQ"
                     /locus_tag="PWY98_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2036325.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28376..30598
                     /gene="purL"
                     /locus_tag="PWY98_000548"
     CDS             28376..30598
                     /gene="purL"
                     /locus_tag="PWY98_000548"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2036326.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30583..32022
                     /locus_tag="PWY98_000549"
     CDS             30583..32022
                     /locus_tag="PWY98_000549"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2036327.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32045..33097
                     /locus_tag="PWY98_000550"
     CDS             32045..33097
                     /locus_tag="PWY98_000550"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2036328.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33094..33672
                     /gene="purN"
                     /locus_tag="PWY98_000551"
     CDS             33094..33672
                     /gene="purN"
                     /locus_tag="PWY98_000551"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2036329.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33669..35204
                     /gene="purH"
                     /locus_tag="PWY98_000552"
     CDS             33669..35204
                     /gene="purH"
                     /locus_tag="PWY98_000552"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2036330.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35220..36494
                     /gene="purD"
                     /locus_tag="PWY98_000553"
     CDS             35220..36494
                     /gene="purD"
                     /locus_tag="PWY98_000553"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2036331.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36616..37530
                     /locus_tag="PWY98_000554"
     CDS             36616..37530
                     /locus_tag="PWY98_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2036332.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37663..39228)
                     /locus_tag="PWY98_000555"
     CDS             complement(37663..39228)
                     /locus_tag="PWY98_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2036333.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39221..40672)
                     /locus_tag="PWY98_000556"
     CDS             complement(39221..40672)
                     /locus_tag="PWY98_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2036334.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40686..41702)
                     /locus_tag="PWY98_000557"
     CDS             complement(40686..41702)
                     /locus_tag="PWY98_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2036335.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            41922..43010
                     /locus_tag="PWY98_000558"
     CDS             41922..43010
                     /locus_tag="PWY98_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2036336.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43064..44590
                     /locus_tag="PWY98_000559"
     CDS             43064..44590
                     /locus_tag="PWY98_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2036337.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44592..45797
                     /locus_tag="PWY98_000560"
     CDS             44592..45797
                     /locus_tag="PWY98_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2036338.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46054..47031
                     /gene="guaC"
                     /locus_tag="PWY98_000561"
     CDS             46054..47031
                     /gene="guaC"
                     /locus_tag="PWY98_000561"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2036339.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47295..48719
                     /locus_tag="PWY98_000562"
     CDS             47295..48719
                     /locus_tag="PWY98_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2036340.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            49180..50289
                     /gene="pdhA"
                     /locus_tag="PWY98_000563"
     CDS             49180..50289
                     /gene="pdhA"
                     /locus_tag="PWY98_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2036341.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50292..51269
                     /locus_tag="PWY98_000564"
     CDS             50292..51269
                     /locus_tag="PWY98_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2036342.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51294..>51363
                     /locus_tag="PWY98_000565"
     CDS             51294..>51363
                     /locus_tag="PWY98_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HDL2036343.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWF"
BASE COUNT        16191 a         8983 c        11260 g        14929 t
ORIGIN      
        1 atcacttcaa cagtttttat tatattatta gggtttgttt gtagaaaaaa ggggattttt
       61 tctgaatctg ttggaaaaat tctatcgaaa gtcgtcttaa cagtggcttt gccagcactg
      121 gcatttcgag ctttcatgca agatatcaat acagaattac tgatgcaagg aatcaatgtc
      181 ttgatttggg gattagttat ttatattatt cttattctag tttcaaaacc ttttttctta
      241 agctataaag gggataagca aacaacgttg cgtgtcctga cgatctttgg ttctacgacc
      301 tttttcggaa cgcctatcgt cagtgcgatt tatggtcctg tcggcgtgat gtattcctca
      361 attttcaata ttggctaccg aatctttttg tattcttatg gatacataaa aatgagtggc
      421 ttgaagatgg aagctaaaaa tatcaaagcg atgttcgtca atccaatcat tatcgctact
      481 tttgccggcc ttttcatttg gctgttccaa gggtatctgc cacaagtgtc agttacaggt
      541 gcagaaggag agataagcca agtagcgttt ttgcgaattg accagactgc accgtggcta
      601 tttcagtcga tggattattt agctggttta agttcaccat tggcgtggct agcaatcggc
      661 gcaacattag gtgaagtaag cttcaaaaaa gctgcatctg aaaaaacttc atggtattac
      721 agtctagtca aagtggtatt tgtacctgca ttgaatatcg tgattctagc agttttgacg
      781 gcaacgaata ttctaccagt caattttgaa gcactggcaa caatcgttat catgatggct
      841 acaccagctg caacagtggc agcagcctat gctatcagtt ttgataggga ggcactttta
      901 gcctccaatg catccttact gtcaactatt tttgctgtgg tactgacacc tgtatggatc
      961 gttgtattac aaatgattca tggcataggt ttattttcat aagaaatgga ggaagaaagt
     1021 atgagaatcg catgttacgg tgttcgtccg aacgagatag atttttttga aaaattgaat
     1081 atttaccatt atgatctcag tctttatgaa gaactgttga cacatgacaa tatcgtgtca
     1141 gcaaaggacc atgatgctgt attgttgagg ggaaattgta tagcagatga gaccaatctt
     1201 gcaaaaatgc aggaatatgg gattcgttat gtttttacac ggactgtggg agtcaatcat
     1261 atcgatctac aagcggcagc tgattttggt atgacagtag cccgtgtacc atcgtattcg
     1321 ccaaatgcga tagcagagtt atctttgact tttgccatga tgcttttgag aaatactgcg
     1381 tatacgacga tccgaacatc tttcaaagat tttcgagtgg atgagcaaat gtttagtcgt
     1441 gaaatcagaa attgcacagt agggatcata ggaacaggca ggatagggtt gacggaagct
     1501 gttttgttta agggactagg agccaaagtg atcggctacg atattcatca gacagaagct
     1561 gctaaaaaag tattgacgtt ccactcgctt gaagagttat tagccaaaag cgatattgtc
     1621 agtattcatg tacctcatat cccaggtgaa aacgatcaaa tgatcaatga aggattttta
     1681 gctcaaatga aaaaaggtag catcttgatt aatacagccc gtggagagct tcaagataat
     1741 gaagctattt taagagcttt gaaaagcaat catttagctg gttttgcaac cgatgttttt
     1801 gcaaatgaaa cagaggtttt tttccgatcc ttcaagccat gggagacgat tccagaccct
     1861 gctatccagc aattagtaga aatgtaccct agagttcttg taaccccgca tgtaggttca
     1921 aatacagatg aggcattatc aaatatgatt tcgacaagtt tcgagaactt tcgtgaaata
     1981 atagaaactg gaaaaacgaa aaatgaggta tctcttccta aagcccgtca attaagataa
     2041 gaaaacatgg atattcgtca ataaaatgtt taaaaatagt ccaagtaaac agttgttact
     2101 tctgtatact tggactattt tgtatgggat aattcgatga ctcaacagta atctgtgagc
     2161 tttttccgcc cattctttgt caattcataa caggttagcg gccggccgat ttccaaatat
     2221 ttgattcttt cgttcaatat atcttgttct actaaaaaat tcaaatattt cttgacggat
     2281 atacgtgaca gtgagctttt ttgagccagt tcttttgttg aaaaaatagt atcttctttt
     2341 agaataaatg cacttatttt ttttaaagtc agttttgaca gacctttagg caaatcgatg
     2401 aatgatggta tttcgtgaat gggagtggtg tggttgaata cttgatcgat caatttctga
     2461 tcggcttttt cagtcgtttg gattgtctga gagaaagcaa gatacttttc gatcgccagc
     2521 tgaaaacgtt gcaaagtgaa cggtttgatc aaataatcta aggcaccgta gcggatcaca
     2581 ttctttatgg tttcgatatc atctgcagca gtgatcaaaa tcactgggat atcatttcct
     2641 tgctgtctaa gccaagataa aaaatggata ccactttcgc caggcatgta tacatcaagc
     2701 agcagcagat ctgcgttttt ttgtctgaaa tatttttttc cttcttctac atttctgaaa
     2761 ctttcgatta cgttcatatt agggatgctt tccacaaaag tacgattcaa ttctgcaacc
     2821 attggatcat cttcgattat caggacattt gtcatcattt tgatcttcct tttttggtaa
     2881 ttctagatag acgattgtac cgccatttgt ttgtttggaa aattctaaaa atccaccgtg
     2941 gcgtttgacg attgaatgga tgagattcaa tccaaaccca cgattgattc ccttcgtcga
     3001 aaaacctagc gtaaatattt tttctgagat ttcttctgat atcccgacac cgtcatcgca
     3061 gacggtcacg atgaatactt cagcttctgt gtcatagttt aaatgaatcc ggacctgtcc
     3121 gtttctttca tgtttttgta gcacggcttc ttttgcatta tcaagtaagt tccctaacac
     3181 ttgcaaaagt gaatggatgg agtcatccat ctccatttta gggataaaag aatcgctttc
     3241 taacagcatt tcaacagatt gttctttggc ttcgtttatt ttgcctaata aaaaaccggc
     3301 aattgcaggt actttgatca gacttgagac ttggccaatc tcttttttat aattttgatt
     3361 tagctgctgg ataaaatggg tgacttcttc atacttcttc aaatcaatca aaccagaaat
     3421 gacatgaagt ttattcatga attcatgggt ttgagctctc atggagttta tatattgttt
     3481 cgtcccccca agctcctcca ttaatttcat tacttctgat cgatttcgta aagttgcgac
     3541 tgctcctaaa aaacggtcat tgctaaaaag aggtgtaacg ctagaaatag tttctatccc
     3601 atttaaatac atggattgag tccgaacggt cgtttgatgc tcaatcgtct ctttaaacaa
     3661 aacgtcatat agattatcac tgattttttt cctacttcga aatccaatag ctgctctgtt
     3721 tggtagaaaa tgttttttgc aggtgtattg atcaagagga tagttttatc agaagagatg
     3781 gctaagattc cttcatctac ttcattttcg ataatcgttt tttcacttaa ctgttctgct
     3841 atttcttgtg gttcaagtcc aagcagtact tttttcattt tttgggcaag aaaaacggcg
     3901 cctatgatgc ctacgctaag tccaataatc aatgaaaaaa caatcatttt ttgtccatct
     3961 ttgacttgat tgtcaatcgt gttgtttgtt aagccaacac aaactgcacc aataatctct
     4021 tgatcataat aaatagggac gaaatagcgg aagccgaacc ctaacattcc ttctttttca
     4081 gagaaaaatt cttgtcctcc aatcactttt ttcacatctt catgacttga gaaaggaaga
     4141 ccgatgtaac ttgggtctgc gtgagaatat cgaatgcctt cagggtccat gacaacgata
     4201 aaatctaaat tcgatgcaat catgatttca tttgttattc cttgaatcgc atgtgtcggg
     4261 tcaccagtct gcagcttttt ttggatgagt ttatcattgg caacgatttt agcaatgcta
     4321 gagattttat ctgacacact agatcggttc atcgaaagaa taaaattcct gatcaatatg
     4381 gcagaaacaa tcaaagaaac gaatgtggag aaacaaacga caaggatgat cgtgttctgg
     4441 agctttaatg gttttttctt aaaaaaagga aacataggct acctcgaaag aatttattgt
     4501 gaaaatttta acatgaaaac aaaaatttaa caaattgtca acgataaaca caaccttcct
     4561 ataaaaaaca ccactgagtc ggtaaaagaa gctatacaga attttcaaac attggtatga
     4621 tagacgtaga aatggaagga ggcgtgtata tgataggggg aatcgcactt gagacgattt
     4681 attggtatac attgctgatt tgtgcaggat tggcagtcct attgattatt tttggagatg
     4741 tattcgattt tgatggcccg cttgatccga tgcttattat cccttggtta actttcacaa
     4801 gcttatttgg ttttctaggt gagcgtttaa cagatcaatc atctttattt ttatttattg
     4861 ctagtggagg catatcgata gtgttggtat ttttgcttaa tttctatata ttgatgccga
     4921 tgaaacatgc cgaatcaacg atgtcgtctt ctgaaaaaac tctggaagga caagtagctg
     4981 ttgtgatcac accgatccct gtacgaggta tgggagaaat tcagttgaaa agtgttaccg
     5041 gttccattag tcgtccagct tgtttttaca gcccccaaga acagacagct gagcggggaa
     5101 cgaaagtatt gatcattgag atcaaggaaa gaatctgtta tgtcatgcct tatgaaagca
     5161 tgataaaagc ttaaaggagc gtgtgttaaa tggaaatacc agttgttttt atcgcgatcg
     5221 caatcgcagc tttgatttta ctgatgttgc tgatcgtctt tgtagcgaag taccagacag
     5281 caaaaccgga tgaagcgttg atcatcagtg gaagttattt aggaagcaaa aatgtgcaca
     5341 ttgatgaaag caataacaaa atcaaaattg tgcgtggagg tggcgcattc gtcttgccag
     5401 tttttcaacg ttcgaatcgg atcagtctat tatcgagtaa attggacgtc tccacccctg
     5461 aagtgtatac ggaacaaggc gtaccggtga tgtgtgatgg tacttcgatc atcaaaatcg
     5521 gttcatctgt agaagagatt gctacagcag cagaacagtt tttaggcaag acaagagaag
     5581 aactggaaaa tgaagcaaga gaagtattag aaggacactt gcgctcgatt cttggttcta
     5641 tgactgtaga agaaatctat caaaatcgcg ataagttcag tcaaagcgta caagaagtcg
     5701 cttctgtaga ccttgcaaaa atggggttga tcatcgtttc tttcactatc aaagaggtca
     5761 gagacaaaaa cggttatctt gactctttag ggaaaccaag aatcgcccaa gtcaaaagag
     5821 atgcagatat agcagaagcg gaagctctaa aagaaacgcg tatcaaaaaa gctcaagcag
     5881 aaaaagaatc acaagcagct gaattacagc gacaaacaga gatcgcagaa tcattaaaag
     5941 aaaaagaact gaaattagca acctacaaac aagaacaaga tgttgctaaa gcaaaagcag
     6001 accaagccta caatttagaa agtgcccgag cacaacaaca agtaatcgaa caggaaatgc
     6061 agattaaaat cgtagaacgt caaaaacaaa tcgaactgga agaaaaagaa atcacacgtc
     6121 gtgaaaagca atatgattcg gaagtgaaga aaaaagcaga tgccgatcgg tacgctaaag
     6181 aacaagaagc acaagctcaa aaagtcaaag aagtgactga agccgaagca gaacgattcc
     6241 gtgtggaagc actggcagaa gcggaagcca ataagacacg tttagctgga caagcagaag
     6301 ccgaagcagc actagcaaaa ggaaaagcag aagccgaagc aaaacaaaag atcgccaatg
     6361 ccttcaagga atatggcgaa gcagctgtct tgagtatggt tatcgatatg ctaccgcaat
     6421 tgatgcgcga agcagcacag ccgctaggaa atatcgaaaa aatctctgtt gtcgatacag
     6481 gaagcagttc aggagaaacc agtggagcga accgagtgac aaattatgcc acaaatttgc
     6541 tttcttctac tcaagaaaca ctaaaagcga caacaggact tgacttgaag gaaatcgtag
     6601 agaatttgtc gacacgtggg actactagcc aagtaaatat gtatgaagcg ccaaaagagg
     6661 cagaacaaaa ctcgatgaag gaagaataaa gtagtacact tcaccgttca atcaaaaatg
     6721 gaggaagact ttttaaagtt cttctctcca tttttttatt ttattgcaaa agaaaattag
     6781 tcctcgctat ttttcttttg tttttggatc ttagcttcta tctcttgtaa atttgttctt
     6841 agcaatcgtt ctttcttttg gagttcgacc aaatcttctg atagttcttc cttcttttct
     6901 gtttcaggag gacgaagaaa gaaattgata ccgattgcaa tgaatgtccc tataaaagta
     6961 tctagtacac gttggatagc atacaaagcg gattcgcctt gagggatgct caacgcaatc
     7021 agcaacaacg tagctattgc ggaaatgata ccggaattat tgttaatacc atctgacacg
     7081 acgatcacaa taataactag aataggaagc aagatcaatt ctaccaaaaa atcattgtga
     7141 aaatagcttt tgacaaaaaa ataaatgata gctaatgtac cacctaatgt attgccgatg
     7201 atcctagact ttccaaaaga aacggttgta gtcaagtcct gacgcaaaga aaaaacagca
     7261 gataatgcag caatcatcgg gattcctcgt tgagtcaaat gaaaaaacaa gatacataaa
     7321 aaaacagcta aggcactttt gatcgtacgc atacctagtc gaaaacggcc aaattgcata
     7381 gagattctcc tttttggtct ttttactatc atacctaaac ttttgtctga tagctagttt
     7441 ttcattattc aagtataatt aaaataaaaa agggggaagt aaatgcgtat aggaatgcga
     7501 acagtcaaaa cagtgatcag tgtcttcttt tcgatggtca cagcgggtag cttatcgctg
     7561 ttgtattggc cagctgcagg gatcattgca ttattaagtg taggaaatac caagcgctct
     7621 acattgcgta cgggcatgaa tcgaatcgca gcctttatat tagctacggc aacagcactg
     7681 ttcagttttt ttgctgttgg gtatactatt tttggcttca gtcttttttt attcctgttc
     7741 atcccgcttg cctctcgctt caaattaacc gaagggatag tagtaaattc ggttttggtc
     7801 actcattatt tagtggagga aaacatgtct tggcagctga tcggcaatga agcgggatta
     7861 atggcgattg gattagtatt tgctttgctt gcaaatgttt acatgccaga tcgtacgaag
     7921 cagctgaagg aaaaccaaat acagatcgaa aaagaatttc gaaatatttt gagacaaatg
     7981 gcagaatttc ttttatcaga aaataaagaa gatgtgcaga tcaaatgcga acatttactg
     8041 acattcattc gcgaaagcca agaagatgcc agagaacatc aagaaaacta ctggctcaga
     8101 cagccgctat actacgaaac ttacttctct atgagaagag cacaggccaa tgtgattaaa
     8161 gacatgctgg aaaatctaga gagaatccag caaccagctt attatggaaa acatatctat
     8221 ggtttattga tttatactgc agaaacattt tcagagtcaa atgatggcag acaaatttta
     8281 acacgtattg aagaagttta tgtattgtat cggcaaatgc ctctgccgac tagtcgccct
     8341 gaattcgagg atcgagcaga attgttccag tttttgcagt cttttaaaag ttttattgaa
     8401 atcaaggcag aattctccca acaaaaaatc caaaaataga ggttgtgaat tcagctaata
     8461 ttcaggtcaa aaagctgatt tcacaacctc ttctttaaac ggtgttctcc tgtcaactcc
     8521 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg
     8581 tgactcggag ctgaacggta ggctcacaac ctctttctta aacggtgttc aaaagctttc
     8641 ctctgcggta ataagtccag ccaaacgaaa aataggagga gctatttttg tttggctgtt
     8701 cttattactt gatgcagaac agctttttca caacctttta aaattacata tttttccata
     8761 gaacttgcat ttgatccaag atttcgatga catcttttgt caagaagaga gagcgattgg
     8821 gcaagtcacc ttcaatcatg tgtaccatat tctttatttc gtaattcatt gcttcagatg
     8881 tattcccgct ttcgatgaat tcctttgtac catctgtaaa taagatctcc gcacgatcag
     8941 cacgcgggta atcagcaatc gtcacataac cgttctcaaa ggcaacgata ccgacttttg
     9001 gcatttttgc ctgaaaagtc aagctgacag cagcaagttc attttctttg ttacgcaaga
     9061 tagtgacgga ttgttcgtcc acgcctgttt caaaaggaac cattgtagag gcaactactt
     9121 caggttgaga gctcaaaaag aaacgagcga aagatacagc atacgtaccg atatccaaca
     9181 gagctcctcc agctaattct ggattgaaga accggttttt agggtctggt tctttgtaac
     9241 tgccgaaagg tgcttggatc atttttaaag cccccagttt tcctgtatcc attattgagc
     9301 gtaactgctg ataaagtggc atgttgaaga ttgtcatcgc ttctgctaag atcaaattct
     9361 tttcttctgc taagcgcata gcgtcagcca gttctttttt attcatcgtg atggcttttt
     9421 cacatagaac gtgtttgcct gcttccaatg ctgccaagat atggtcgata tgctgtctat
     9481 ttggtacagc aatgtacacg atgtcgattt cagcatcgtc taacatttct tgatagcttc
     9541 cgtaagcttt tggaatattg taacggtgag cgaagttttc cgctttttct gaagttctgg
     9601 aagcaactgc tgttagttcg ctcgtctctt gattgaagct ttcggcaaac tcatgtgcga
     9661 tggtaccaag accaacgatt ccccattgat attttttcat ctgaaaacgc tccttcatag
     9721 ttggattgag tttcttgtct gtctattatt ataacaaaaa tcacgaagaa taaagaggag
     9781 aggacttgaa acattaaaaa aaatgaaagc agcgatttaa gaggtgcttt ttgatcaaaa
     9841 cttaagtaga atagttaaaa ggaggggata aacgtgcatc aagtatatat tgatcttatt
     9901 atgagtgtat tattagaaca gtttgaaact gaaaaaaatt ttttagatga atacttgctg
     9961 atcagtccag aacaatggca aaaatggaaa gatggcgaga aaaatctgcc atctgaagcg
    10021 atgcagaaag taaaaaatct tttcactgat tatgaatgga tgctaacgca aaagattttg
    10081 cgtcaaacga ttatttttcc cgaaaaacga aacgttgccg ttgcagagta caagcaaatc
    10141 aaaacacgca tcgcgcaaaa atggcttagc agcaaagcag caaaagtcga gctgatccct
    10201 ttgaaaaata atcagcaggc tggtagatac cttgacttga aagtaagtat tcaatatgat
    10261 gaatggggct atgatgatat tttgaatttt cgtctgccag cagatgttca aaaacagatc
    10321 gaaggagaaa aaatcgaact tctcgattgg gtgaatgaaa atctggaaga aacttacgtt
    10381 catgaggcga atggggaacg tgaagaacaa cgaaaggatt tttcaagtga ataaattttt
    10441 aggatttgga gcagttgtga ttatgatcgc agcaatgggc tatgttattt ttagcgatga
    10501 tgattcgcat gttcaagccg gcaatcaagc agtcgaacga acaagcacca aaacaactgc
    10561 tagtaaagaa aagaacagtc aaacaaccag cagcaaggta aaggacaaag aaacaaaaga
    10621 tcgtacgtcc aaacaattgc cagatgttaa aacagacgat tggaagctag ttttggttgg
    10681 tccagatcac aaattagaaa aagaaattga tgagtctact caattggcat cattaagtaa
    10741 cggatacttg atcgacaaac gcattgctcc agaatatgaa gcatttgcca aggctgctga
    10801 agcagctgga tttccattag tgatggtctc agcttaccgg tctgtatcat cccaacaaca
    10861 agttttcgaa caaaatgtcc aagatgtgat gagtcgtcaa ggtgtatcag aagaggaggc
    10921 gacaaagatt actaaagaaa cgattacgga gccaggatac agcgaacacc acacaggtct
    10981 ggcagtcgat gtcgtcgatc aaaactggta caacagttat ccttctactg tattggatgc
    11041 aagttacgga gatcagcctg gagctaaatg gatcgcagat aatgctgcgc agtatggatt
    11101 tatcgttcgt tatccaaaag gacacgaaga tataactaag atcacatatg aaccttggca
    11161 cttacgttat gtcggtaaag aaaacgctga gtatatcacc aaacaccaat tgacgctaga
    11221 agaatatctg aatgaattaa atgaaaagta ggaattgatc atggcgaaaa aaaagtggaa
    11281 aaagcaaaag cgcaagaata tccgttggcc agcagtggtt gccggtgtag cagtgatact
    11341 cttagccttt gttttttcct tgaagaatct gtcttctcct tttactgata atcagcaaga
    11401 aaatgaagcg caatcccatc agcaatttat tgaacggctt gttccgcatg cccaagaact
    11461 acaagatggt tatggagtgc ttcctagtat cattttagga caagctatat tagaatcaaa
    11521 ttggggaaaa agtaccttgg ctagcaaata caataatctt ttcgggatca aggcttatgg
    11581 ggatcagaag aaagtaagcc tggaaacaaa ggaatttgtt aacgaagaat ggataactat
    11641 ccaaggagac ttcaaagtgt atgattcatg ggaacagtcc atggatgacc atacacaact
    11701 gttcgtccaa ggagtagatt ggaatcctgc gctgtatgaa aaagtgatta cggccaccaa
    11761 ctatcaggaa gccgcacaag cattgcaaga tgcaggttat gcgacagacc ccggttatgc
    11821 gcaaaaaatc atccaggtca tcgagacgta tcaattgaat cagtatgacc ggagatagca
    11881 aaggagaagt ggctgatggc agaacaattt acgccagaaa tcatgacgga attgagaaaa
    11941 gatatcgtaa gagtaccgga aatcatttta gaagcaagcg ggatcaagat atttggaaaa
    12001 ttgatccgtt cgatcatctt tactacggat attgcgatca tacgtaatac aaatgcgaat
    12061 gcagtaattg ctgtgtatcc ttttacacct catccagcga ttactaaaag cattattgag
    12121 gcagctgaca ttccagtttt ctccggtgta ggtggtggtt tgacacaagg tgctcgttct
    12181 tcctacatga gcatgttcgc agaagcgcaa ggttcgttag gggtcgtttt gaacggtcct
    12241 acgcctttag agacagtaaa agaagtttgt cgagtagttg atattccagt tatcagtacg
    12301 gtaacgtcga aatatacaga aatagatgaa aagttagcag ctggtatttc gattatcaat
    12361 atcagtgcag gtaaagaaac tgcagaagca gtacgccatt ttcgtaaaaa atatcctgaa
    12421 cttcctatca ttgcgacagg tggtccaacg gaagaaagta ttgctgagac cattcaagca
    12481 ggagcaaatg ccatcacata tacgccacca agtagcggag agctttttag tcgtaaaatg
    12541 gaaaaatatc gggaaactga gaaaaagaat catgaataac aggtgagaat caaaagacga
    12601 ggaagagcag atagtggctc ttcctcgtct tttgtactcc agtactattt gttcagaatt
    12661 caaaaaaaag agaaattttt ttgttttttt acctgtaatg tacataagaa gagttaaaac
    12721 cttttgttat tattagcatt ctaacaaaaa aatgtcttga tttattgaag cgctttctat
    12781 agaatgaaaa acgaataaaa actaaaaaag gcacccgaca atttagtagc agctaaattg
    12841 tcggggcttg aaaagtcagc gggaactgac caatcaagtt gccatagtca atgcttgtgc
    12901 atcgacgtgt atattgtacc taatttcata cagaattact agttattttt ctgtttttta
    12961 gggaagagcg cacacgaaga agcatgctta tttagctatg tcaaggatat tgggcagttg
    13021 tctgatatcc ttttttcttt gcgtaattat cctcccatca ttgaagcaag aagtgttttt
    13081 agcgatgaaa gaagaatcag cactatctaa tttttcttga aagtattgag ttgttcatcc
    13141 taggcagaac gataaggcaa gtcttggttt ttattcccct tgaaaaagat cgtttaataa
    13201 atatgttctg tataaaaata cagtatattg aaaaataaca atggaagtag aggtgcagag
    13261 aatgaagaaa agaatgagaa tcatcttagc tttaagtgtt atgttattct tcttgacggc
    13321 ttgtgggcaa gaagatgaca aaaaaatcac gctgaacatg gcttggtggg gttcgcaagt
    13381 ccgacacgat gcaacagtaa aagtaatcga gctttatgaa gaaaaaaatc cccatgtgac
    13441 gatcgattat gaattttatg attatgaagg atattacaca aaattaggta cactagcagc
    13501 atcagatgat atctgggaca ttttccaact gaacaatcaa tttcctcaat acatcaatca
    13561 gattgaacca ttagatgaat atgtggagca agggataatt gatatttcta atgcagatga
    13621 atcctattta gcaacgacga cttacgacgg gcaattagtt ggactgtcta acggggtgaa
    13681 ttcgtttgcg attgcttaca ataaaaatat atttgaacaa ttaaatatcc ctgaaccagc
    13741 ttcaaattgg acatgggatg aatttgaaca aatcagctat caaatcactg aagaattagg
    13801 aatctatgct atttcaagat tcgaagattt tatagctggc tgtattattc aaataccaca
    13861 agttgagaaa ggattgaatt tttataatcg ggaagatcta tctaacagct tgggatttga
    13921 aaatccagac tatctaacag attttttcca gctgcgtaaa aatctggtag acaaaggggc
    13981 ccatccagaa ccaggtggtg gagctgcaag tacgacggat ctgtcttttc aggcggttag
    14041 ggatagagaa gcggcaatgg tgtttctatc aagtaaccag ttgacagaaa tattgtcagg
    14101 ggctcctgaa gatatggaga ttcagttgat taatccacct cgcagaaaag cagatggaga
    14161 atctggtatc cctttacgtt cttcgcaaat gctgagtatg gcaaaaagct cagcccataa
    14221 agaagaagct gctaagttta ttgatttctt tcaaaacagt gaagaagcca atgaaatttt
    14281 aaaaggagaa cgtggtgtac caatcatgtc gaatattcga gaacagctcg ctcgtgcaga
    14341 tgatgcaact ttgcaagcga catttgatta tttagaaatg atttccgata tggacaacgg
    14401 cgaagtgaat gtctttgata gtgctttgaa tccggaaatt caagaccaat acaatctttt
    14461 agttgaaaaa gtcatttatg aagaagtaac agcagaagaa gcagcagaga gtctttatga
    14521 gtttgcgtca aacattctag gatagcagca ggaggaaggc atgaaaaaag aaaaactaaa
    14581 acggttcttg gatcaagaca atgtggtcgg ttacgtattt gcttctccat tcatcctagg
    14641 atttctattg ataacagtga taccgatgat cttgtcactt tattattcgt tcaccgatta
    14701 tcgtttggga aatccagtct catggatcgg cttggataat tatataaaga tgctgagtga
    14761 tgcccgtttt ttgaactcca tccgagtcac ttttctctat gtatttgcat ctgtacctgt
    14821 gaaactgatc tttggtctat tgattgctta tcttttaacg aggaatatca aaggaacgac
    14881 tttctttcgt tctttgtatt atgtgcctag tttgatcggt ggcagtattt ctatcgctct
    14941 agtttggcgg gagttgtttt ctaaaaacgg gttagtgaat attttcctgg aacgattagg
    15001 cgcacctgcg ctttcctggt ttagcgatca aaaactggtc atgatccctc ttgttttgat
    15061 gtctgcttgg caattcggtt cggcaatgat catctttgct gcaggtatca aagagatccc
    15121 aggctcgtat tatgaagcgg cagagatcga tggagcgaac aaatggcaga cgttttacaa
    15181 gatcacgtta cctcttcttt cgccgatcat tttgtataac ttggttatgc agacaatcgc
    15241 ggcgtttatg acttttacgc aggcatttat cattacccaa ggtggtccga atgatgccac
    15301 gaacttttat gctctttatg tgtataacca agctttttct tttaattcta tggggtatgc
    15361 tagtgctatt tcgtggatca tgctgttgat tatgggagct gtcacctttc tgattttcaa
    15421 atcatcaaaa aaatgggtcg ttgtgtgaat gagaggagga agaaatgaat aatacagtaa
    15481 cgaaatggtt tcaatacggt tttattttgc tattaggtct agtcatgatt tatccggtct
    15541 tatggatgat cgcaggttct tttaaaacgg atcaagagat tatgagtggt tcgctgaatt
    15601 tgattccaga gacttttcaa tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa
    15661 tttcattttt tactttcttt aggaattcgt ttgtcatctc tgcagtttcg acgataggga
    15721 cagttctgag ctcgacttgt attgcttacg cattatcgag aatcgatttc aaaggaaaaa
    15781 gattctggtt cgtcgtcatg ttagtaacga tgatgattcc tgcacaagtg attttgattc
    15841 cgcagtttat tatttacaat cggctgaatt tggttgggac gtatgtgccg ctgattttac
    15901 cacatttttt tggacaagca tttttcattt accagatcat gcaattcatg gtcagcatcc
    15961 ccaaagagtt agatgaatca gcgattattg acggatgcag caagtatagt gtctttacca
    16021 aaattatttt tcctttgctg aaaccttcga tcatcacgac gatcatcatc cagttttatt
    16081 ggaaatggga cgacttcatg ggaccattga tttatttaaa caagccgaga tcttacacgg
    16141 tatcgattgc catcaaatta tttgcagacg ctggatctag tacgacttac gcgagtatgt
    16201 ttgcgatgtc tacgttgtcg ctgctaccgg tttttctgat tttcttattc ttcaataaat
    16261 atcttgtcca ggggatcagt acaagcggtc tgaagggctg aatatgaaaa agccaataga
    16321 agagatgttt ctttacaagc tcaactacta tgtctgtcgg atttttttaa ccagtcttat
    16381 cagtaatgct cttttcgctt tagttctagg atgtttgttc actttaggat tagtgctgtc
    16441 tgctcgtgtt gtctatctga tttatttttt gatgatgtat ccttcttatt atctatctgt
    16501 ccattacttg gcagaaaaaa aggtatgttg gaggaaagga aaaatcaaac ggatgtgttt
    16561 catcattacg ttgtttcagc tgtttgtcat tatttttgca gttgattggt atttattagt
    16621 gggaaagatt ggagcttggt atctgattcc ttttctattg tgtctgttgc tttttgtatc
    16681 ccatagtttt tttagtacgc taattgatat ctgcaaagaa gataaagcag atagtcttgt
    16741 ttatttgatc aaaaggaata tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta
    16801 tttatgcctt ttgtatggac tgttctggtg tatacagcat gattttttac tcactgcatt
    16861 actgtatcca gggctgccat ttgtcacagt attttgctta aaagaaaagg agaatgactt
    16921 taaatgaaga aaaaatatgt acaagtcgga cttggtggga gagcgcgttt tttttatgag
    16981 gcaattgcct ctacgtatca tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa
    17041 acaaggatgg attatgcaaa tcggatactt gtgaacaagt atggacagaa aaaagtaaag
    17101 acatgtatgc catcacaatt tgagcaacta ctggatgacg tacggccggc tgaagtcatt
    17161 attacgtcag tagatcgaac gcatcatgat tatatcattc gtgcaatgaa aaaaggatat
    17221 gatgttattt gcgaaaaacc tctgactatc gatgaggaaa aagctcaaga aataataacg
    17281 gtccaaaaac agacgggaag aagcctgcga gtcactttta attatcgtta tgcaccgtat
    17341 cacacgaaga taaaggaact gatagaaagt gatgtgattg gtgatgtgtt ttctatccac
    17401 ttcgaatggc tgttaaatac gcaacacggg gcagattatt tcagaagatg gcatcgaaat
    17461 aagcacaata gcggcgggct tcttgttcat aaatctaccc atcattttga tctagtgaat
    17521 ttttggttga acagccgtcc ccaaaaggta tttgcattcg gtggattaaa cttttatggg
    17581 atgcataatg cggagaaacg cggagtagaa atattttaca gtcgatgcta tggaagcgaa
    17641 ctagctaaag ctgatccatt tgctatagac ctgtcagcca atgaggaatt gcgagaaatg
    17701 tatttagaag cagaaaaaga cagtggatat attcgtgacc gcagtgtatt ttccgatgat
    17761 atctctattg aagatacact aggcgtaacg gtcaaatatg aaactggtgc gattttgacg
    17821 tattcgctaa acgcgtatat gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa
    17881 ggacggatcg aaatgaaagt gatcgaaaag tcttacatca atgcaggtgg agataaaaag
    17941 gatgaaggag cagccgttct tcagtccatc catgtctatc cgatgttcgg tgagccatat
    18001 gaggtaatcg tcgaacaaga agcgggcgga catggcggag gagatccgcg tttattagat
    18061 gatctatttg gtgaagaaaa agaagaagat ccgtggaatc gggcagcaac tcacgtagat
    18121 ggtattttgt cgattttgac aggaatcgca gcaaatcatt ctattgcttc aggaaaagca
    18181 gtagtgattg acgagcttgt cagcttaaaa gaataagaat agatttacgg aaattacttc
    18241 ccgtagttta taaaatcttg ccttattgcc gataggctgt cttttgaaca ctatttattc
    18301 ggaaaaaggg gctatgacat gctttttgtc atgccttctt tttatgtggg ctgctttttt
    18361 agataggaga aaataaaaat aactatttaa acccgaacaa aaaggaataa tgcattaaaa
    18421 atcattagat tttacaaaaa aataagtaaa ccacgttgtc agtttcagaa agtagtgtta
    18481 gactgttttc aatatttgaa acaacatttt ttattcaaat atggcaaact gccttcacgt
    18541 gcaaaacagt tgcaagaaaa tgaatttaca ctattattag ttttacatac ctacttatat
    18601 atcgtcataa tatggatgac agtttctagc cagtaccgta aatgctggac tataagtaaa
    18661 agattggcta tttcaccctt cttttcttat gataaagtgg agggtgtttt tctttaacag
    18721 aaacatagta ggtttttgca caaaagcggt tatttcggta ttaataaatg ataaaggagc
    18781 aaataaagtg aaagaattag ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg
    18841 cgtattaaaa gtcgacagct tcgtcactca ccaagtagac cctgtattga tggaacaaat
    18901 gggaaaacgg tttgcagaag tatttaaaga acaaggaatc acgaaagttg tgaccattga
    18961 agcttctggg atcgcaccag ctttatttgc tgcaagagaa ttagatgttc caatgatctt
    19021 cgctagaaaa gcaaaaagtt tgacgatgga tgaagaatta ttgacagctt ctgtttattc
    19081 ttttacgaaa caagtgacaa gtacgatttc tatttctcgt aaatttttat cttcagaaga
    19141 caaagtattg attatcgacg atttcttggc aaacggacaa gcggcaaaag gattgatcga
    19201 attatgccag caagctggag cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt
    19261 ccaagatgga cgtcagttac ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat
    19321 tgcttccttg acgaatggac aagttgagtt tcttgaggag gacgcataat cgtggaaaaa
    19381 gaaacacaaa atggaaaagc tgcggtttta gggctgcagc atctgctagc gatgtatgca
    19441 ggagcagtag cggtgcctct tttgatcggt acaggactag gctttaatca agaacagatg
    19501 acttatttga tttcaattga tatctttatg tgtggattag cgacattatt gcaattgaca
    19561 gttaaccgat ttttcggaat tggcttgcct gtcgttttag gatgtgcgat ccaagcagta
    19621 gctccactga tcttgatcgg aaccaatgaa ggagtaggag ctatctacgg atctattatc
    19681 gcttctggga tctttgttgt gttagtttct ggtgtctttt ctaaaattaa aaaactattc
    19741 cctcctatag taacaggaac ggttatcacc gttattggtt tgactttgat tcctgtagca
    19801 atcgagaaaa tgggtggagg taatgcaaca gcagctgatt ttggtgacaa aacaaatctg
    19861 ctacttgcat ttgtgacgat tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc
    19921 attcgttcaa tcgcggtatt gattggttta gtcggaggaa gcatcttagc tgctttcatg
    19981 ggaatgattg atctgagcgc gattggagat gcgccgttgt tccatgttcc acaacccttt
    20041 tattttggta aaccaacttt cgacgtatgg tcgattttac taatgatcat catttccatg
    20101 gtcagcatgg tagaatcaac aggtgtatat tttgcacttg gtgacattac tgggaaaaat
    20161 gttggagaag aagaattgaa aaagggatat cgtgcagaag gacttgccgt tatcttaggc
    20221 ggtattttca atacattccc ttatactggc ttttctcaaa atgtcggatt ggttcaactt
    20281 tcgggaatca aaacaagaaa acccatctat ttctctgcat tcttcttgat tgttttagga
    20341 ttattcccta aaatcggtgc gttagcacaa atcatccctg agccggtact tggtggagga
    20401 atgttagtca tgttcgggat ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat
    20461 ttcagcaatg ataaaaatct attgattgtt gctgtttctg ttggatttgg gctgggtttc
    20521 aatgttacgc cgacattatt cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat
    20581 ggcatcgtaa tgagtagcat tacggctatc atattgaatt tgatctttaa tggattgaaa
    20641 aaagatgaag aaatattgga agaagccttc aagaagtaat agagcaaaag aaaggttgtg
    20701 aaatcagcgt tttgacctga ttgttagaga aacaaacgga aaaatagttc ctcgtatttt
    20761 gccgtttgtt tgagctaata ccgagggtca gctgattgaa caccgttgaa ggaagaggtc
    20821 gtgagcctgc cgttcatctc cgagtcacaa ggaacaattt caaaaaaaag cttctcatgt
    20881 ttgttgaata ttgttccttg tgaccgagga gttggcagga gaacaccgtt tatccataaa
    20941 aaggaatgtg acctaacttt tgtcacattc ctttttttta tctttaataa acacgaacaa
    21001 taaaatgttt ttctttgtta acattcgaaa tttctttgac atcaaaaaaa gtgattgtta
    21061 aaatatgaat gtagaaaaac tgggaggaaa aacaatgaca gcaaaaatct ctgtgattat
    21121 ggggagtact tcagattggg aaacaatgaa agaagcatgt caaatattgg atgaattcgg
    21181 tgtgtcttat gaaaaaaagg tcgtttctgc tcatcgaacc ccagatttga tgtttgaata
    21241 tgcacaaaac gctcgaaaaa aaggaatcaa aatcattatt gcaggagcag ggggagccgc
    21301 gcatctgccg ggtatggtcg catcaaaaac aactttgccg gttatcggtg taccggtcca
    21361 atcacgaaca ttaaatggtt tggattcgct tctttcgatt gtccaaatgc ctggaggagt
    21421 acctgtagct actacagcaa tcggaaaagc gggagcaatt aatgctggac tacttgcgat
    21481 acagatgctt tccatgtatg atttagaatt ggaggctaaa ttggctgaaa gacgttcaat
    21541 gttggctaaa actgttatag aaagtagtga tcaacttggt taaacctttg ttgcctggtt
    21601 caacgatcgg gatcgtcggt ggcggtcaat taggtcgtat gatggcgatt agtgcaaaag
    21661 aaatgggctt taaagtaggt gtgctggatc ctgtgagcga ttgtccagcc gcacaagttg
    21721 cagattggca cattgaaagt gtctatgatg acacctttgc gctgaaagag cttgccagaa
    21781 gaacagatgt cattacctat gaattcgaaa acgtcagtgt agaggcgtta aatgcgattt
    21841 tacccatgtc atttattcct caagggacag acttgcttgc gattacgcaa gaccgcctgt
    21901 tagaaaaatc ttttttggaa acgaataaca tcgtgattgc tccttatgca acgatcgtca
    21961 gtccgacgga tatccaagat gcgattgacg ggatcggcta tccttgtgtt ttgaaaacca
    22021 ctcgaggcgg ctatgatggc aaggggcagt atgttttgaa aagtcgggcg gatctagctc
    22081 ctgccatgga ccttttaaga gaaggaacct gtgttttaga agcatggatc ccgtttgaaa
    22141 aagagatttc gatcatggtg gcaggaaatg gtcaaggaga tttcacgact tttccggtcg
    22201 tggaaaacat ccaccacaac aatattcttc atgaaacgat tgctccagcc gcgattgatc
    22261 aggatgtgat cgaggaggct gagcgtattg cccgtgtgat tgcagaagca gtgagccttt
    22321 caggtgtttt aggggtagaa atgtttttaa caaaaacagg cgggctatat gtgaatgaat
    22381 tagctcctcg tcctcacaat tctggacatt attctattga agcttgttct atgagtcagt
    22441 tcgatgccca tattcgaggt atttgtggtt ggccattagg agaaaaagta cgtttgataa
    22501 gcaatgcagt aatggtcaat gttttgggag aacaactgct ggaaagctat cgcttgattg
    22561 agaataagcc agactggcaa ttccatttct atggaaaaaa acaagcaaaa aaaggtcgca
    22621 agatgggaca tatcactatc ttgacagaag acatccagca aacgctggaa gaaatcaaac
    22681 aaacgaatat ttgggattag aggagaaaaa acatgttaga acgttataca cgccctgaaa
    22741 tggggaatat ctggacggaa gagaacaaat accgtgcgtg gctcgaagtc gagattttag
    22801 cagatgaagc ttgggcagaa cttggtgaaa tcccgcaaga agatgtgaaa aaaattcgtg
    22861 agcatgcatc atttgatatc gatcggatct tggaaatcga aaaattaaca cgccatgatg
    22921 tcgttgcgtt tacacgagcg gtttctgaaa cattgggaga agaaagaaaa tgggtccatt
    22981 atggtctaac aagtacggat gtcgtagata ctgcttatgg ctatctcatc aagcaagcaa
    23041 atgatatttt acgggaagac ttgaaacgtt ttacggaaat cattggcgaa aaagcaaaag
    23101 aacacaaata tacagtgatg atgggtcgga cacacggtgt ccatgctgaa ccaactactt
    23161 ttggattgaa acttgccaca tggtattcag aaatgaaacg gaacatcgaa cgatttgaac
    23221 atgcggcaaa aggagtagaa gcaggaaaaa tctcaggagc agtgggaacg ttcgcaaata
    23281 tcccgccatt tattgaagag tatgtatgta aacaattggg gatccgtcca caagaaatct
    23341 ctactcaagt tctccctcgg gatctacatg ctgaatattt ctcagcgatg gcgcttattg
    23401 ctacaagtat tgaacgtttt gcaacagaga tacgcggatt gcaaaaatcc gaaacacgtg
    23461 aagtagaaga attttttgcg aaaggtcaaa aaggttcttc tgctatgccg cacaaaagga
    23521 atccgatcgg ttcagaaaat atggctggtc tagccagagt ggtgcgcgga cacatggtca
    23581 ctgcctatga aaatgtcaca ttatggcatg aaagagatat ttctcattct tcagcagaac
    23641 gaatcatctt gcctgatacc acgattttat tgaattacat gttaaatcgt tttggcaata
    23701 tcgttaaaaa tctaacggtc tttcctgaaa acatgaagcg gaatatgtct gcaacatttg
    23761 gtttgattta tagccagcgc gttatgctga aattgatcga taagggaatg acaagagaag
    23821 aagcttatga tcttgttcag ccaaaaactg cgtacgcttg ggacaaccaa gtgcagttcc
    23881 gcccgctgtt agaagaagat ccaaccatca catcggtatt atctaaagaa gaaatagacg
    23941 atgcctttga ttatcatcat catttgaaaa acgttgacgt gatattcaaa cgagtaggtt
    24001 tggaataaag aagagtgagt tgcaaaattc aattaaaata gaatagcaaa aataatcgag
    24061 tagaggggaa agccaactga ccttccgccc ctctcacacc accgtacgta cggacccgta
    24121 tacggcggtt caataagttg agtgtacgaa ctggtacttc aaggagatgt cctcatatcc
    24181 ttggcgggcc agttctttgt ctgttaatga tcgatgaaga atatgacttt tggagactga
    24241 ccaaatacct tttcttgtat ttgaccattc atacgctttc gctttatcga tccctaaacg
    24301 aatcagattt tttcttttgg ttcttggatt cttccattgt ttccaaatat attgtctgat
    24361 tcttcgcttc aaccaaccgt ttaactccct catgaattct ttcatctcac caattccata
    24421 gtagttgatc caaccagtca ttagttgctt gatttctttt aaaataagtt ggatacttct
    24481 tccacggttt cttttagtga gttgtttgag cttgcttttc acacgctgtt ttgcttttgc
    24541 gtgagggcga attttaatac ctttctttgt tggcagaatg cagaatccaa gaaactttcg
    24601 tttcaatgga cttcctaccg cactcttttc tcgatttacc gtcaacttta gttgttcttc
    24661 gagaaattga atggatttct tcatcacacg ctgtcctgct cgtttgcttc taacatagat
    24721 attgcagtca tcggcgtatc gaacaaattt atgtcctaga ttttctagca cttgatcaaa
    24781 ttcgttcagg tagatgttac ttaacagggg agagagattg cctccttggg gcgttccttc
    24841 ttcagttgtt gtgatgagtc cattttccat tacaccactc attaagtatt gacggatcag
    24901 ttttaggact tgtttgtcct ttactttccg ctcaatgaaa tacatcagct tatcgtgatt
    24961 tacggtgtca aagtaggctt tcatatcaat gtccaccaca tatcggtaac cttgttcata
    25021 ataagatttt gctttcttca ctgcgtcatg tgcgctgcga tttgggcgaa acccataact
    25081 attatctgag aattctggtt caaatagcgg ttgtaacact tgattgattg cctgttgaag
    25141 catacgatca ataaccgtgg gaattcctag ttttcttaca ccgccgtttg gtttcggtat
    25201 ttctactcgc aaaacaggct gaggtttata gttcccttgg cgtaaattgg atagtaattc
    25261 ctcgcggtgc tctttcaagt aaggaagcag ttgatcgacg gtcatcccat ctgcaccagc
    25321 cgccccttta tttctgacta cttgaagata cgcttgattg agattattcc gatctagaat
    25381 tctcccaaac agattttcta caccatcttg tttcctctgt tcaccgcaag aagtgctgcg
    25441 cacttcatca tacccttcgt gttccacact agctctttga tgatagccaa gttttcttgt
    25501 tttctgcggt cgtcgcatct tctcacacct cctaaattat tcaagattct tattgttcag
    25561 tccttcgtaa cggtcagtgt cgttactact atgacctcgg ctgacttctc atgattcacc
    25621 atcatgtcgc catgatcgtt gtgctggttt gttttcattc aacgcccaca acaccctcat
    25681 gagatctccc ccggtaagag tgaaaacttt cctcccatgt aactgctaga tttactgtat
    25741 aggtttcggg cagtattgga ctttgtcttg tgatgaagac ttatccgacc taattcagcc
    25801 ttgtatctag tttctgttcg tcagttcagg agtttgcgtc cgacttcctt cagccacaac
    25861 ctcacgattg ccgccttgtc tttcgctaac agttcctact gccaagcctg tagtggactt
    25921 tcaccaccaa gttttcaccc atgcagggcg cacaccaaaa aagaagcaga tccgttagcg
    25981 gttctgcttc ttttagttta ttatattttc ttatcaataa taggaacctg attttaaaag
    26041 cggctacctc tttaccgtgc ctctttacat acaaaaaata gaggaattaa gcgtaaatgg
    26101 cttggctatt ataactaaaa tgctggttat ggagatgttg cagcaagttt tcgagaaaag
    26161 cgtattccat ctcttgtcca ttgaataaga tctggatacg ataatgattt ttgaaacgct
    26221 tctgttcatg aatctgactg attgaaagtt cgcctttgtg ttctgtctga taaagaaaag
    26281 tccttttacg gcattcagca cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa
    26341 aactagtatc tttattgcat aaataaacgt ctttgatcga cagcaaatca ttatatagac
    26401 aggaacgttc ttcagctgtt aattctgata aaaaaagctg accttgtttt attgcatctt
    26461 ccatcggcaa gtgcctcctc gaacccatta tccaaatttt tagttaattc taatatacca
    26521 tcattaagta taaatgtaaa cgcttgttga tggtataaga ttttcgtttc tgataaaaca
    26581 cgaacattaa aagaatctaa tgccttaaat gtacatattt ttgttgattt ataaaaagat
    26641 gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa
    26701 ataaaaagga gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa
    26761 aatatatctt acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc
    26821 gttgaatggc gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac
    26881 aagtgcaatt ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt
    26941 gtctaaaacc gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg
    27001 aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat
    27061 caaaccaatc attgaattct actataagga tgaccagttg gatgatccat ttatcaatga
    27121 agagcatatc tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca
    27181 agcgttggct attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt
    27241 agatttcaag ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac
    27301 accggatact tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta
    27361 ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga
    27421 actaactgaa gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa
    27481 gattcagtgt tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat
    27541 gaaacaatcg aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa
    27601 gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg
    27661 gaaacatacc gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat
    27721 cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg
    27781 cgctgatgcc gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt
    27841 actgccggga ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc
    27901 tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg
    27961 caatggattc cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga
    28021 gtctttgcat tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac
    28081 ttcagagtat gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta
    28141 tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta
    28201 taatgacaac atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa
    28261 tgtgttagga atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga
    28321 tgggaaacgg ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa
    28381 aacattgaaa gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg
    28441 attgactgat gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta
    28501 tacggaaact ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa
    28561 accggttcta cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg
    28621 cgcaggcatc gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa
    28681 tcatccttca gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag
    28741 agatattttc agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga
    28801 attagataat ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata
    28861 cggcaattgt atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga
    28921 aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa
    28981 agggcaagcc gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga
    29041 cggtatccac ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg
    29101 ttctgctgta caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga
    29161 attaatcttg catcattcgg attctctaat aggtatacag gatatgggag cagcaggact
    29221 tgtttcatct agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga
    29281 tgaagtccca caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca
    29341 agaacggatg ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca
    29401 gaagtatgat ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct
    29461 atatcatcaa ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc
    29521 agtttatcat aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc
    29581 ttatcagcca gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac
    29641 gattgcttca aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt
    29701 tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc
    29761 tatgaccaca gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat
    29821 agcagtggca gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac
    29881 cgactgcctg aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc
    29941 agctgacgga attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt
    30001 ctctttatat aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt
    30061 cggattgatt gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct
    30121 aatctatata ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca
    30181 gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca
    30241 agagttagta ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga
    30301 aggtggcttg gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt
    30361 acagtttgaa ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac
    30421 agtcgcaccg gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc
    30481 aggtaaggtg acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat
    30541 agaaactgca gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag
    30601 agtttaaatg aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg
    30661 gcatacttcg ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc
    30721 aatgatcgag gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa
    30781 gatcaaagaa aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca
    30841 acagcaggca atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag
    30901 tcgatcggct tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta
    30961 gaggaaaatg gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc
    31021 cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa
    31081 ggtggctttg cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat
    31141 ggttttcgtc cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa
    31201 acttgtgcat tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata
    31261 gtgatcatca atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct
    31321 atttgttcga tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat
    31381 gtgcatactg cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca
    31441 gatatggtcg ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct
    31501 agtggaatcc cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc
    31561 cagccaacgc ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga
    31621 gtagtggaag gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc
    31681 agacggatcg tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct
    31741 tctcctcctt tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc
    31801 attgcggcaa atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt
    31861 tttttaagtg aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca
    31921 ggattgtgta tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa
    31981 aaatatcagg aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga
    32041 gtatatgaat tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata
    32101 tgaagtagta gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg
    32161 cgctatagga ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt
    32221 ccttgtctcg gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa
    32281 acacgataca atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg
    32341 cgcagagccg ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt
    32401 ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg
    32461 cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt
    32521 tgctgtaggg atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga
    32581 tgtgttgctt ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa
    32641 aattttcttt gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac
    32701 acttggcgaa gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat
    32761 caaagaaaag aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat
    32821 cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct
    32881 tccaatattc ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat
    32941 cttcaatatg ggcatcggta tggtcctggc agtccaagaa tcgaaagtaa aagaaatagt
    33001 acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga
    33061 aaaacatcgt gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga
    33121 aacggaagca actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg
    33181 attgactggt tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg
    33241 agtgtaccgg cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa
    33301 gcaattttgc ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg
    33361 cgaatcattg gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg
    33421 tctcttttac ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta
    33481 aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc
    33541 cggcaggaaa aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc
    33601 catagagtag agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc
    33661 tatctgaaat gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg
    33721 cccaaggttt gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt
    33781 tggataaagc tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga
    33841 tggatggccg tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg
    33901 atctccctag ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt
    33961 gtgtcaattt atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag
    34021 ctattgaaaa cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg
    34081 aatttgtcac ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc
    34141 agtatgggca tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata
    34201 ctgcggcata tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac
    34261 cggaaaaatt gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc
    34321 atcaagccgc tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa
    34381 aacaactcca tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac
    34441 ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg
    34501 gtattgggac agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg
    34561 tttcaatctt tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac
    34621 agatgcataa gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg
    34681 aattactgca aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa
    34741 atcaaaatcg agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc
    34801 tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag
    34861 aagcaaaagc attagcgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg
    34921 tactggcaaa cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt
    34981 cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg
    35041 atgcttattt tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa
    35101 tcattcagcc aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg
    35161 atctagcaat ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa
    35221 tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag
    35281 ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg
    35341 gtatcaattg tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga
    35401 aagaggctat tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg
    35461 atgattttat ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg
    35521 aaggttcgaa aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat
    35581 ccagaagttt tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa
    35641 tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga
    35701 aggaagcgct ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca
    35761 aaaaaatcgt cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc
    35821 gtaataccaa ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg
    35881 ataaagggcc aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa
    35941 agatgattga aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac
    36001 taggacgctc tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa
    36061 aagtgatcga attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt
    36121 tgaagagtga ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa
    36181 agtggcagca agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag
    36241 aatatgagac aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg
    36301 ctggagtttc ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag
    36361 aagcatacgg tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata
    36421 aaacgacaga tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac
    36481 tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag
    36541 cgccgatttt agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa
    36601 taaggaggag ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca
    36661 gtgatcttag gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca
    36721 gcttatgaaa acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt
    36781 gaagaaatct ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt
    36841 caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt
    36901 gaatctgtag aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt
    36961 catcgtccag atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa
    37021 cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg
    37081 ctgaaaaaat atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat
    37141 actggaatga tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat
    37201 gacggtagtg tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca
    37261 taccagtatg gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac
    37321 caaacattag aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct
    37381 tggattttac gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga
    37441 atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg
    37501 taccgtgctg ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat
    37561 aaattaaaaa gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt
    37621 ttattgcaaa tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt
    37681 aaggaaaagc tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata
    37741 actgaaatag ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc
    37801 tgattgctgc aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa
    37861 tgtttggtta aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg
    37921 ttgagcgacc caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa
    37981 tgcatggttc aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa
    38041 gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa
    38101 gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca
    38161 gacatgctgg agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt
    38221 gccttgtggt ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga
    38281 caaatcaagt gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat
    38341 caaatcctca tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg
    38401 cgtgatctca aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac
    38461 agaaaaatta tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata
    38521 actggtctcg atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga
    38581 ggaaacagca gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact
    38641 gagatgagcg taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat
    38701 ttttttccct gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt
    38761 aagtgagcct ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc
    38821 agaagaagtg tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc
    38881 ttgtttcaat tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat
    38941 tgctcctttc atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg
    39001 aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc
    39061 agtcagaatg atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc
    39121 agcttcgccg acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa
    39181 gttattgcga atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc
    39241 caattgtttt gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc
    39301 tgtcaatcgt gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt
    39361 tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta
    39421 tttctgccag tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca
    39481 tctcatcaat tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc
    39541 catggtatac agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa
    39601 aaagtgtttc tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga
    39661 tgactagata atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt
    39721 tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa
    39781 agcagtcttg ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag
    39841 taatctgagc ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt
    39901 tctcccgtaa agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt
    39961 gtaattccag tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt
    40021 ctgacatctc gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat
    40081 tctttttcgc atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa
    40141 tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa
    40201 aatcttgtaa gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt
    40261 cattggtatc cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt
    40321 ccagtgtatc gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg
    40381 tattattttt gatctctcga atatcattcc taagttcttc taggatattt tcctcattga
    40441 actcatctac ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat
    40501 caaccttcaa agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca
    40561 aaagctgttg cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca
    40621 tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc
    40681 tccagtcaat aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg
    40741 gaaataaaaa tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta
    40801 acggtattgg ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt
    40861 cctgagcgta gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct
    40921 tctccaggtt tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca
    40981 ctatggctgt ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc
    41041 tggtcttttc ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga
    41101 ggtttatggt cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa
    41161 aatccttcgg agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt
    41221 ctgatttcct gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt
    41281 ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga
    41341 atatgtctgt cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag
    41401 tcccacccgg attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac
    41461 gtccgtatat cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg
    41521 taatcttctt tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc
    41581 tccgttcctg attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt
    41641 gtacacccca tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc
    41701 actttaaatc actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca
    41761 aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa
    41821 agtacaccta aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta
    41881 ttagctatac tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa
    41941 agattgttgg gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt
    42001 caggatctgc agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag
    42061 aaagatggat cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa
    42121 cagatcttca atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga
    42181 ttacaaaagg ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg
    42241 tgttagctaa agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga
    42301 attcagaaaa tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag
    42361 cagcttatat tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat
    42421 tatttggtgg ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg
    42481 ttttacaacc atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca
    42541 gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt
    42601 taagcgcaaa ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa
    42661 gtttaggaat catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac
    42721 ctgtgattac tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac
    42781 aaacacaaac gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca
    42841 aagcaatcag tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg
    42901 tgaaaactgt ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag
    42961 cagagctgat cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa
    43021 acaaggaact aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt
    43081 aaaaatggca gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct
    43141 gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac
    43201 attgatgaat gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta
    43261 tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat
    43321 cattcatcaa gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg
    43381 aaatgaacaa gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga
    43441 actgctgaat aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt
    43501 agggcaacag caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat
    43561 tttggatgag cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat
    43621 ccgagagttg aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat
    43681 cgtggatatc gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga
    43741 aaatgaaatg atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga
    43801 tcgttatcct gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac
    43861 ggtctatcac cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa
    43921 aaaaggagag attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat
    43981 gagtctattt ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc
    44041 agagatatca gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga
    44101 ggaccgaaaa tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag
    44161 cagtttggat aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc
    44221 agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag
    44281 tcttagtggt gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga
    44341 tattcttttt ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta
    44401 cacgattatc gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact
    44461 tcctgaaata ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg
    44521 agaagtctcg cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga
    44581 ggtgggttaa gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt
    44641 tgaaaagtag agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac
    44701 tacttattgc gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta
    44761 accttgttct gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc
    44821 tcttaggtca tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg
    44881 gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag
    44941 taggcgggct gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt
    45001 ttatcgtgac tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag
    45061 gccaatctct agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag
    45121 acttaagcga aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga
    45181 tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag
    45241 tggaatctcc aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa
    45301 taacttatat ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga
    45361 tcgttgcagc atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga
    45421 cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct
    45481 gggtattcgt caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat
    45541 tgaatgcggc aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat
    45601 attttggtgg tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg
    45661 gcttggtcat gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc
    45721 agcaggcaat caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga
    45781 aaaaacgaac aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt
    45841 gtcacagtct ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa
    45901 taatatatgt ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta
    45961 attgagttaa attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt
    46021 tttgctggat gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt
    46081 caattaattc ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca
    46141 ttaggtaaac atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat
    46201 gaaacaatcg cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat
    46261 gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc
    46321 agtgttggcg tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta
    46381 aatcctgatt atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg
    46441 attcagcata ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca
    46501 ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt
    46561 ccaggaaaag tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg
    46621 gcagctctta gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc
    46681 agaacacatg gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt
    46741 tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg
    46801 tataaagaat actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa
    46861 ggaaagaaaa tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa
    46921 caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa
    46981 gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat
    47041 aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa
    47101 aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa
    47161 catcattatt cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc
    47221 aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata
    47281 gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa
    47341 ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg
    47401 aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat
    47461 tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta
    47521 atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc
    47581 ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa
    47641 aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg
    47701 agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag
    47761 gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat
    47821 tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac
    47881 aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat
    47941 tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga
    48001 tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca
    48061 acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag
    48121 tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt
    48181 catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat
    48241 acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac
    48301 ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata
    48361 tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag
    48421 atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat
    48481 cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact tgggatggga
    48541 aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa
    48601 gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa
    48661 accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat
    48721 ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct
    48781 tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc
    48841 cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt
    48901 taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat
    48961 aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa
    49021 tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat
    49081 ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg
    49141 aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg
    49201 atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt
    49261 gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc
    49321 gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg
    49381 aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg
    49441 gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct
    49501 caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct
    49561 gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca
    49621 caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc
    49681 gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat
    49741 ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc
    49801 tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct
    49861 gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa
    49921 atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat
    49981 cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca
    50041 gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa
    50101 ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa
    50161 gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac
    50221 atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag
    50281 tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg
    50341 aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag
    50401 ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc
    50461 ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc
    50521 cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc
    50581 aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt
    50641 cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga
    50701 tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac
    50761 tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat
    50821 atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag
    50881 cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag
    50941 caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc
    51001 gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg
    51061 tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga
    51121 tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata
    51181 cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag
    51241 acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt
    51301 atcaatttaa attgccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt
    51361 tcg
//
LOCUS       DANKAY010000010        49923 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000010 DANKAY010000000
VERSION     DANKAY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..113)
                     /locus_tag="PWY98_000566"
     CDS             complement(<1..113)
                     /locus_tag="PWY98_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2036344.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLK"
     gene            complement(115..312)
                     /locus_tag="PWY98_000567"
     CDS             complement(115..312)
                     /locus_tag="PWY98_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2036345.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(706..2433)
                     /locus_tag="PWY98_000568"
     CDS             complement(706..2433)
                     /locus_tag="PWY98_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2036346.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2426..3619)
                     /locus_tag="PWY98_000569"
     CDS             complement(2426..3619)
                     /locus_tag="PWY98_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2036347.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3806..4450)
                     /locus_tag="PWY98_000570"
     CDS             complement(3806..4450)
                     /locus_tag="PWY98_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2036348.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4544..4678)
                     /locus_tag="PWY98_000571"
     CDS             complement(4544..4678)
                     /locus_tag="PWY98_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2036349.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4680..6812)
                     /gene="feoB"
                     /locus_tag="PWY98_000572"
     CDS             complement(4680..6812)
                     /gene="feoB"
                     /locus_tag="PWY98_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2036350.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6809..7282)
                     /locus_tag="PWY98_000573"
     CDS             complement(6809..7282)
                     /locus_tag="PWY98_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2036351.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7683..7907
                     /gene="nrdH"
                     /locus_tag="PWY98_000574"
     CDS             7683..7907
                     /gene="nrdH"
                     /locus_tag="PWY98_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2036352.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8066..10225
                     /gene="nrdE"
                     /locus_tag="PWY98_000575"
     CDS             8066..10225
                     /gene="nrdE"
                     /locus_tag="PWY98_000575"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2036353.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10275..11240
                     /gene="nrdF"
                     /locus_tag="PWY98_000576"
     CDS             10275..11240
                     /gene="nrdF"
                     /locus_tag="PWY98_000576"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2036354.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11329..12468)
                     /locus_tag="PWY98_000577"
     CDS             complement(11329..12468)
                     /locus_tag="PWY98_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2036355.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12777..13490)
                     /locus_tag="PWY98_000578"
     CDS             complement(12777..13490)
                     /locus_tag="PWY98_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2036356.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13744..14445)
                     /gene="nagB"
                     /locus_tag="PWY98_000579"
     CDS             complement(13744..14445)
                     /gene="nagB"
                     /locus_tag="PWY98_000579"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2036357.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14679..16211
                     /locus_tag="PWY98_000580"
     CDS             14679..16211
                     /locus_tag="PWY98_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2036358.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16402..17604)
                     /locus_tag="PWY98_000581"
     CDS             complement(16402..17604)
                     /locus_tag="PWY98_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2036359.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17686..18375)
                     /locus_tag="PWY98_000582"
     CDS             complement(17686..18375)
                     /locus_tag="PWY98_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2036360.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18481..18849)
                     /gene="rplL"
                     /locus_tag="PWY98_000583"
     CDS             complement(18481..18849)
                     /gene="rplL"
                     /locus_tag="PWY98_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2036361.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18902..19405)
                     /locus_tag="PWY98_000584"
     CDS             complement(18902..19405)
                     /locus_tag="PWY98_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2036362.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19609..19736)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19815..20504)
                     /gene="rplA"
                     /locus_tag="PWY98_000585"
     CDS             complement(19815..20504)
                     /gene="rplA"
                     /locus_tag="PWY98_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2036363.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20608..21030)
                     /gene="rplK"
                     /locus_tag="PWY98_000586"
     CDS             complement(20608..21030)
                     /gene="rplK"
                     /locus_tag="PWY98_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2036364.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21373..22041
                     /gene="sdaAB"
                     /locus_tag="PWY98_000587"
     CDS             21373..22041
                     /gene="sdaAB"
                     /locus_tag="PWY98_000587"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2036365.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22054..22926
                     /gene="sdaAA"
                     /locus_tag="PWY98_000588"
     CDS             22054..22926
                     /gene="sdaAA"
                     /locus_tag="PWY98_000588"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2036366.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23017..24036)
                     /locus_tag="PWY98_000589"
     CDS             complement(23017..24036)
                     /locus_tag="PWY98_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2036367.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(24209..25114)
                     /locus_tag="PWY98_000590"
     CDS             complement(24209..25114)
                     /locus_tag="PWY98_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2036368.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25173..25718)
                     /gene="nusG"
                     /locus_tag="PWY98_000591"
     CDS             complement(25173..25718)
                     /gene="nusG"
                     /locus_tag="PWY98_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2036369.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25819..25989)
                     /gene="secE"
                     /locus_tag="PWY98_000592"
     CDS             complement(25819..25989)
                     /gene="secE"
                     /locus_tag="PWY98_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2036370.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26013..26165)
                     /gene="rpmG"
                     /locus_tag="PWY98_000593"
     CDS             complement(26013..26165)
                     /gene="rpmG"
                     /locus_tag="PWY98_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2036371.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26327..26971
                     /locus_tag="PWY98_000594"
     CDS             26327..26971
                     /locus_tag="PWY98_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2036372.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27027..27530)
                     /locus_tag="PWY98_000595"
     CDS             complement(27027..27530)
                     /locus_tag="PWY98_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2036373.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27543..27589)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28127..29092
                     /locus_tag="PWY98_000596"
     CDS             28127..29092
                     /locus_tag="PWY98_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2036374.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29191..30363)
                     /locus_tag="PWY98_000597"
     CDS             complement(29191..30363)
                     /locus_tag="PWY98_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036375.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30350..31495)
                     /locus_tag="PWY98_000598"
     CDS             complement(30350..31495)
                     /locus_tag="PWY98_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036376.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31509..32433)
                     /locus_tag="PWY98_000599"
                     /pseudo
     CDS             complement(31509..32433)
                     /locus_tag="PWY98_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32550..33629
                     /locus_tag="PWY98_000600"
     CDS             32550..33629
                     /locus_tag="PWY98_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2036377.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33661..34254
                     /locus_tag="PWY98_000601"
     CDS             33661..34254
                     /locus_tag="PWY98_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2036378.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34364..34591)
                     /locus_tag="PWY98_000602"
     CDS             complement(34364..34591)
                     /locus_tag="PWY98_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2036379.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34694..37282)
                     /locus_tag="PWY98_000603"
     CDS             complement(34694..37282)
                     /locus_tag="PWY98_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2036380.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37302..37961)
                     /locus_tag="PWY98_000604"
     CDS             complement(37302..37961)
                     /locus_tag="PWY98_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2036381.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38010..38513)
                     /gene="trmL"
                     /locus_tag="PWY98_000605"
     CDS             complement(38010..38513)
                     /gene="trmL"
                     /locus_tag="PWY98_000605"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2036382.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38734..39600)
                     /locus_tag="PWY98_000606"
     CDS             complement(38734..39600)
                     /locus_tag="PWY98_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2036383.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39709..40350
                     /locus_tag="PWY98_000607"
     CDS             39709..40350
                     /locus_tag="PWY98_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2036384.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40412..41773)
                     /gene="mgtE"
                     /locus_tag="PWY98_000608"
     CDS             complement(40412..41773)
                     /gene="mgtE"
                     /locus_tag="PWY98_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2036385.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41791..42690)
                     /locus_tag="PWY98_000609"
     CDS             complement(41791..42690)
                     /locus_tag="PWY98_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2036386.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42692..43489)
                     /locus_tag="PWY98_000610"
     CDS             complement(42692..43489)
                     /locus_tag="PWY98_000610"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2036387.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43467..44171)
                     /locus_tag="PWY98_000611"
     CDS             complement(43467..44171)
                     /locus_tag="PWY98_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2036388.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44302..44878
                     /locus_tag="PWY98_000612"
                     /pseudo
     CDS             44302..44878
                     /locus_tag="PWY98_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44996..45673
                     /locus_tag="PWY98_000613"
     CDS             44996..45673
                     /locus_tag="PWY98_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2036389.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45752..47560)
                     /gene="pepF"
                     /locus_tag="PWY98_000614"
     CDS             complement(45752..47560)
                     /gene="pepF"
                     /locus_tag="PWY98_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2036390.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47736..48734)
                     /locus_tag="PWY98_000615"
     CDS             complement(47736..48734)
                     /locus_tag="PWY98_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2036391.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48960..49613)
                     /locus_tag="PWY98_000616"
     CDS             complement(48960..49613)
                     /locus_tag="PWY98_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2036392.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14946 a        10479 c         8764 g        15734 t
ORIGIN      
        1 aattttaaat ataccttttg taaaacctca agaatatcag aatatttagg atctttactt
       61 atagatatat cattatataa ttcatgaaca atttgcttag catttttttt cattttagca
      121 cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt
      181 tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata
      241 ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt
      301 taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt
      361 cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa
      421 tttaatcact tttaagtata taaactatgt atattcttta atattttttg agaatgcact
      481 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
      541 cgcacccata aattcataat agtttgtcgg cgtctttgga cgtttcgaag atgagctacg
      601 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      661 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      721 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      781 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
      841 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
      901 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
      961 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
     1021 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
     1081 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
     1141 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
     1201 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
     1261 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
     1321 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
     1381 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
     1441 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1501 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1561 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1621 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1681 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1741 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1801 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     1861 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     1921 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     1981 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     2041 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     2101 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     2161 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     2221 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     2281 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     2341 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     2401 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     2461 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2521 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2581 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2641 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2701 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2761 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     2821 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     2881 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     2941 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     3001 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     3061 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     3121 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     3181 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     3241 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     3301 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     3361 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     3421 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3481 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3541 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3601 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3661 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3721 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3781 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     3841 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     3901 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     3961 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     4021 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     4081 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     4141 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     4201 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     4261 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     4321 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     4381 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     4441 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4501 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4561 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4621 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4681 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4741 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4801 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     4861 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     4921 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     4981 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     5041 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     5101 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     5161 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     5221 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     5281 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     5341 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     5401 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     5461 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5521 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5581 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5641 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5701 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5761 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     5821 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     5881 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     5941 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     6001 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     6061 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     6121 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     6181 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     6241 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     6301 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     6361 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     6421 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6481 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6541 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6601 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6661 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6721 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6781 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     6841 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     6901 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     6961 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     7021 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     7081 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     7141 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     7201 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     7261 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     7321 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     7381 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     7441 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7501 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7561 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7621 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7681 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7741 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7801 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     7861 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     7921 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     7981 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     8041 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     8101 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     8161 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttctatc
     8221 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     8281 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     8341 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     8401 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     8461 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8521 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8581 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8641 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8701 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8761 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     8821 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     8881 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     8941 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     9001 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     9061 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     9121 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     9181 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     9241 tgtgattaac ggcaaacagg aatatgaagt attaggaacg gatatctcat gtaacttagg
     9301 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     9361 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     9421 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9481 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9541 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9601 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9661 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9721 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9781 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
     9841 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
     9901 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
     9961 tatggacatg cgtaaagtaa tcgatgttta tgcaatggca caacaacacg tcgatcaagg
    10021 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
    10081 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
    10141 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
    10201 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
    10261 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
    10321 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
    10381 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
    10441 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10501 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10561 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10621 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10681 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10741 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10801 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    10861 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    10921 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    10981 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    11041 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    11101 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    11161 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    11221 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    11281 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    11341 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    11401 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    11461 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11521 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11581 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11641 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11701 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11761 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    11821 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    11881 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    11941 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    12001 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    12061 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    12121 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    12181 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    12241 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    12301 ataatagata aaaccagcaa caagcaatgg taaaaaaact gccgaaagca ttttcaatat
    12361 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    12421 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12481 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12541 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12601 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12661 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12721 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12781 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    12841 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    12901 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    12961 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    13021 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    13081 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    13141 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    13201 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    13261 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    13321 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    13381 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    13441 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13501 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13561 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13621 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13681 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13741 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13801 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    13861 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    13921 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    13981 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    14041 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    14101 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    14161 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    14221 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    14281 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    14341 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    14401 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    14461 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14521 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14581 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14641 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14701 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14761 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    14821 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    14881 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    14941 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    15001 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    15061 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
    15121 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    15181 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    15241 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    15301 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    15361 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    15421 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15481 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15541 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15601 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15661 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15721 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15781 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    15841 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    15901 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    15961 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    16021 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    16081 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    16141 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    16201 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    16261 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    16321 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    16381 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    16441 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16501 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16561 gtacataaac ataaatacag tattatttcg gttgcttgct gcttcaccta gcccctgtac
    16621 tcgtgtttga ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa
    16681 aagcaaaatg gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat
    16741 aacaagtccc atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat
    16801 aggtgcagca actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc
    16861 ccaattgtag gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt
    16921 tccaaacatg aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa
    16981 atgcggcaaa cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga
    17041 acgcggcatt ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa
    17101 atagatcatc cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa
    17161 aatccccacg agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc
    17221 cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa
    17281 aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac
    17341 ggcagataac gctgccattc gaatgatcaa ttttggacga tccaccacgt cccctaaagg
    17401 aactaaaaac agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac
    17461 tgcacttgtg ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata
    17521 catattggca acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa
    17581 aggttttttt tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta
    17641 tggaaaagat tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg
    17701 ttttccttgt tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt
    17761 aacacttgca accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc
    17821 acacgcatac aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg
    17881 tagaggcaat ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat
    17941 catatagcct aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca
    18001 atgattcgtt tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt
    18061 gatgttacca agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct
    18121 caaaccagca atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc
    18181 tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag
    18241 ctctgcctta ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata
    18301 caaccgatag cccactacca gtatcagaat gattcccaac gagatcaata tcaatcggat
    18361 tattttcttt ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac
    18421 ataaaaacaa aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa
    18481 ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc
    18541 ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc
    18601 tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc
    18661 agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc
    18721 agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac
    18781 taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt
    18841 caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca
    18901 attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc
    18961 ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt
    19021 ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga
    19081 tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat
    19141 cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc
    19201 cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag
    19261 catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa
    19321 cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt
    19381 tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag
    19441 ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca
    19501 tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc
    19561 gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa
    19621 aaaactctat gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg
    19681 gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca
    19741 actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac
    19801 attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt
    19861 gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat
    19921 acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg
    19981 attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact
    20041 tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag
    20101 ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca
    20161 acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct
    20221 acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa
    20281 acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct
    20341 gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca
    20401 atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca
    20461 acttttttta atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga
    20521 ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg
    20581 agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt
    20641 tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa
    20701 ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc
    20761 gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt
    20821 tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc
    20881 tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc
    20941 ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa
    21001 tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg
    21061 ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa
    21121 ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat
    21181 ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa
    21241 caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat
    21301 gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat
    21361 gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg
    21421 atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt
    21481 agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa
    21541 acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc
    21601 gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg
    21661 tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa
    21721 ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct
    21781 gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac
    21841 caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat
    21901 atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa
    21961 gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac
    22021 agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta
    22081 gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg
    22141 agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa
    22201 caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat
    22261 gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta
    22321 aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt
    22381 gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat
    22441 cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg
    22501 ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt
    22561 ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat
    22621 caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc
    22681 gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct
    22741 tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc
    22801 gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa
    22861 ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg
    22921 gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac
    22981 gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtatattt
    23041 tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag
    23101 gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg
    23161 tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg
    23221 acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg
    23281 caatacttca aaggacgcag gacctccatc tagttgacgt ccaccatgat tggataccca
    23341 gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt
    23401 tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc
    23461 ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact
    23521 ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac
    23581 gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc
    23641 tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa
    23701 ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc
    23761 ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc
    23821 ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa
    23881 ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg
    23941 catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga
    24001 accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag
    24061 ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt
    24121 agatccttct ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc
    24181 aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct
    24241 gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt
    24301 tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta
    24361 acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc
    24421 gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga
    24481 cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat
    24541 taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat
    24601 acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt
    24661 tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat
    24721 tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc
    24781 cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt
    24841 ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc
    24901 cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt
    24961 ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac
    25021 ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt
    25081 tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc
    25141 caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa
    25201 tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt
    25261 tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc
    25321 acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc
    25381 aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa
    25441 ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg
    25501 atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca
    25561 tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt
    25621 ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat
    25681 gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt
    25741 aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag
    25801 tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc
    25861 cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt
    25921 acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag
    25981 aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat
    26041 tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg
    26101 actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt
    26161 gccatttact tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta
    26221 actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat
    26281 gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac
    26341 acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc
    26401 attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa
    26461 tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga
    26521 tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac
    26581 ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag
    26641 tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc
    26701 ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga
    26761 tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct
    26821 tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga
    26881 agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga
    26941 aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat
    27001 ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct
    27061 aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg
    27121 ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc
    27181 atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg
    27241 ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca
    27301 tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa
    27361 agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc
    27421 ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg
    27481 ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct
    27541 cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac
    27601 aataattata acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa
    27661 aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa
    27721 ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg
    27781 tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg
    27841 tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta
    27901 agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca
    27961 ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa
    28021 aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa
    28081 caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta
    28141 tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat
    28201 tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg
    28261 acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga
    28321 agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt
    28381 acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc
    28441 agcaggttct ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg
    28501 acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga
    28561 aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc
    28621 tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg
    28681 atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat
    28741 tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac
    28801 gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc
    28861 agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct
    28921 tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag
    28981 attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa
    29041 acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag
    29101 gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac
    29161 ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg
    29221 atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac
    29281 taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc
    29341 actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct
    29401 aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt
    29461 gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca
    29521 agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac
    29581 cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa
    29641 caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt
    29701 aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaatgcta gattgatgac
    29761 gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc
    29821 cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac
    29881 cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa
    29941 cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc
    30001 atctgggcga ctctgtatct tgatcgtcgg catctttcct acttcgactt gttcggtaaa
    30061 gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc
    30121 tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag
    30181 gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga
    30241 ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac
    30301 acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt
    30361 cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga
    30421 tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca
    30481 aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa
    30541 tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca
    30601 ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca
    30661 atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc
    30721 aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata atataaatct
    30781 ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc
    30841 taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat
    30901 tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa
    30961 aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa
    31021 aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta
    31081 taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg
    31141 cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat
    31201 ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt
    31261 tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat
    31321 ccgctttgat tacaattcca gtattaatcg tttcgagatt gccaccttga tcaatctcgc
    31381 caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca
    31441 tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt
    31501 cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac
    31561 gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc
    31621 ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg
    31681 attccatcag aagctattgt tcgctgacaa cacgttttct gggagtaatg ggatgtcgat
    31741 cgtcaaacga tccgccgtcc gtaccatatc ttttagagat tctttggttc ctttagcaat
    31801 cacctgtcct tgatccatga tcacgatctg gtcgcaaaga atttctactt cttccatgta
    31861 atgtgttgta tagacaatgg ttgctccttg tcgattcagc tccttgatac tttctaaaat
    31921 tttattccga ctttgggggt ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg
    31981 cttgtgggca atcccacatg cgatgttcaa ccgacgtttc aagccaccac taagttctcc
    32041 tggacgaaac gaaacatatt tttctaatcc cactaattga atcacctctt caatcaaggc
    32101 tttccttttc tctttttcgg aaacatataa accgcaaaaa taatcgatat tttcttgcac
    32161 acttagttcg tcaaagaccg caacgtcttg aggaacgacc ccaatcattg ccttgacagc
    32221 ataattttca ggagtcattg ttgtcccgaa caatttaatg gttcctttgt cataatttaa
    32281 caaagaaagc atacagttga tggcagtcga tttcccactg ccgtttggtc ctaacagacc
    32341 taagacttct ccttcttgta cttgcagatc aaaatgattc aatgctacaa aatctccata
    32401 gcgtttgact aatttatcaa tttcgataat catcatgaaa aactccttcc aaatttcttg
    32461 ttgatctgac tataataaag tgcaagaaaa gaaactagtg acgattggct aaaccacaca
    32521 tgacatttgt cacaatgaag gagtctgcta tgcataaatc tcggctactc aacgaaacta
    32581 gctttctact ggttatctca ttgcttttgc tggtaagtca aggattcgtt gctatgcaag
    32641 ttatctattt attgatcgcc gttttattca ctggtgtcag ttatttactg cctacaaagc
    32701 caaattggat tttccaagtt attctatttc ttttatgtgc ctttttacct gatcttgtcc
    32761 tgtttctgcc agcgacgatg cgagtgatcg cccctgaaga aagccacctt gattacttgt
    32821 ttttgttagg ttttttgttg atttcgacgg ttggtaatca tccccccatc aaggtcgcct
    32881 ttgtcgtaat tttgctgttt ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg
    32941 atcttgagaa aaagtacatt gtctccaaag atactcattg ggaaaaacaa caagagttat
    33001 cggaacaaaa taaagcattg gccctgtcac aagaaacaca attagccttg caaattgtgg
    33061 aagaacggaa ccgaattgcc cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg
    33121 cgattttaca aattggtgcg cttgaaatga tcaatcaaga accaaaacta aaagaaccat
    33181 tagaaaattt aagtgccacc gttcacactg ggatggatcg gattcgtcaa agtgtccatg
    33241 acctccatga gacatcgatc tcttttcaac actccttaga gtttctgatc gctgattttc
    33301 cttcttctgt tacgattgaa ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact
    33361 gcttcttaat ggtcatcaaa gaagccttaa ccaacagtat caaacatagt catgccaaaa
    33421 ccgttgtttt acattttcga acgttgccgg ccttttatcg cttgcaaatc acgaatgatg
    33481 gcaccagccc acaaaaaaat tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg
    33541 ttcaagaaca aaagggacaa gtccatattt tccaagaaaa ccagcaattt cagttaacga
    33601 tcattttacc aagggaggga caccaatgat aacagtagtc attattgaga tgatccattt
    33661 gtgacgaaat cgttacaaac cattttagaa agcacgaaag agatccgtgt gttaggcatt
    33721 ggtcattgtg ccaaggatgc gctagtctta tacgaaaccc atcgtcccga tgttttgctg
    33781 acggacatcc gcatgcctaa acaaacgggc atcgatgctg ccaaagaaat tttggctaaa
    33841 tttcccaaag ccatcctttt gcttttgacg acattcaaag atgaagaata catccgcgaa
    33901 gcattttcca tcggtgtcaa agggtattta atcaaacaga acttgcaagc gattattcct
    33961 tctgtcaaat ccgcttataa tggacaggcc gtatttggta atgaaatcat cgagactttc
    34021 actcaaatga tgaagaataa accgacgatt gatagtcatg cttatgcttc ttttagtgaa
    34081 cgtgagcttg caatcatcaa agaagtagct gccgggaaaa acaataaaga aattgcagat
    34141 gccttgtact tgagtgacgg cactgttcgc aattacatca gccaattact agaaaagcta
    34201 gatttacggg atcgcacgca attggctatc tatttttatc agcatttgca ataagtctta
    34261 tctaacacaa aaaaacctga atgatgctcc ttcgcatcat tcaggttttt ttcacatctt
    34321 ctattcaatt gtcccgcaaa ccatttcctc acgattgaaa gaatcaagga ttcgcagggc
    34381 tgccaggatt cgttggattg ttcggcgtgt taggcggaaa aataggcgcc ttgtctaacg
    34441 ttttttctag ttttgattct tcacgatcgt ctcgttctac atcaggactt tcattcaatt
    34501 tgaattcttt ttccatctca tctaactttt taaagtcatt gccagattct tttgctaatt
    34561 tcgctaattt atcttcggtt tctttagcca taaagtaacg cccccctttt gatttcagct
    34621 tagcatatca cactgaaaaa acaaaagaaa acgctaacca acgatcattt ttagctattt
    34681 ctcatcttta aaatcaagca ttttttgccg gtataaaatc gtagggagaa ctgttttcca
    34741 taccaatcat cggatctact aactggttat cgatcacttc tgctgtaaga acgaaatctt
    34801 tagaaacagc aggttctacc tcttctttct tttcttctcc aacaaatgaa gaaaacagat
    34861 ctggttccac tgtttccatt acttggtttt tcgtttcatt cgtcccactg tcatcttcta
    34921 caggtagctt ttcttttaca acccgtggct cttttgtata gttcccatct tcaaatggat
    34981 catttccgac caaagaatct tcataagctg ctagtttggt ccggagagtg atattcattt
    35041 tatcattatc catgatcctt gatttcaacg tagtcatccg gcttgctgat tcattcgcta
    35101 cacaagtgac ataggcttgc tcttccccta acaaaatcaa cagttcctta ctgcgagtca
    35161 ctgccgtgta gagcaaatta cgctgaagca tccgctgata ttgatggacc atgggcaaaa
    35221 taaccatctt gaattcactg ccttgtgatt tatggattga gcaacaataa gaaagagtga
    35281 ttttattcca ttcgtttctt ttataagtga cctcatttgc atcaaactgg ataactaatt
    35341 catccacttt atcttcagaa tcttttgcca agatgatacc cgtgatttct cccatatctc
    35401 cattgaatac attcaattcg ggagtattga ctaattggag gactttgtct cctatccgat
    35461 aaacggtgtc gttccaatgt acttcctttt ttccaggttt ccctggattg aatatctcct
    35521 gcatcatttt atttaacgca tcgatccctg caggtccccg atacattggt gctaatactt
    35581 ggatatcttg tgcacggaat cccttatttt tcgctttttc cacaatttta gagatcaagg
    35641 gttcaatttg ataagcatca cttcggaaat aggaacgatc tttttgattc tttgtgaaat
    35701 cttttggcag acgtccttct ttgatctcat gggcaagagg aataatacta gaaccatcac
    35761 cttgccgata aatctgattg agttccattt tgggaatttg ttcgatttcc aaaagatcat
    35821 ggagtacttg accaggaccg acagagggaa gctgatcttt gtctcctact aagataactt
    35881 gcatattcgt cggaatcgct ttgaataatg tgttagccag ccatgtatcg accatagaca
    35941 tctcatcaac aatcaaaagt ccgccttcta attctttcgt actgactgaa ggtgtttttt
    36001 cccgtccatt cagccccaga agacgatgga tcgtactggc aggcagacct gtcgtttcgt
    36061 tcattcgttt agctgctcgg ccagttggtg ctgcaagtag aataggaaaa atttcttggg
    36121 tatattttga aggatccaat tccagtccgt tcaactctgc aaaaagattg acgattccgt
    36181 tgatcacagt agttttcccc gtacctggtc ctcctgttaa aataaataga ggggaacgga
    36241 ttgcttcagc gatagcttgt tcttgcgaag gtccatactg tatgccgaat attttttcga
    36301 tacgccgaat attcttctgc acttcttttt caggataatg gatttctttt ttacgactga
    36361 gaaggcgctg gatcgaatta ccgatccccc attcagaaaa gtacaaactg ttttcgtaaa
    36421 gattagtctc ttcttgttgt atctttcctt cttcaacaag ttggataatt acagtagcaa
    36481 cctgatcagg ctggatttca attgggcgac ttgtttctaa aatacggatc gtttgctgaa
    36541 gcaatgtctc cgccggtacg tatgtatctc ctgtttgaac tgcatgctga aaaatctgat
    36601 gcaagatagc tgctcgaatt cttctcggtg aatctgctgc aataccgatt tgttcggcta
    36661 gattatctgc acgtttgaac ccgattcctt ctatgtcttc gactagctgg tacggatttt
    36721 cttcgatgat atccaaagac tcgttcttat aggcctggta gatactgaaa gctaactgac
    36781 taccgaaccc gtaacgattc agtcccatga tcacttgatc catcccgtgg tttagccgaa
    36841 tcgtctcagc aagcatttct ctttttggct tcgtcaaacc ggagatttgt tttaataccg
    36901 aaggatcagc aatgattttt tcgatagcat cttctcccag caattctaca attttttctg
    36961 ctgttttctt cccgattcct gggaattttt cgctggaaag atacgctacg atcccatttg
    37021 cagttgttgg cttttcttgc tcatatcttt cgagtttaag ctgccttccg tatttaggat
    37081 gatccactaa ttctccataa aaacgataag tctcattttc ttgaatatcg ccgaaacttc
    37141 cggtgactac aatctctttt tcacggtaat cagctgtggt ttcactgatg tgtaccaaaa
    37201 gcactttgta aaaattacta ggattttgaa aaaaaatcgc ggcaactgta ccaacgaaat
    37261 aaggcatttc ttctggctgc atcggaagcc gcctccttct tctatagtaa aggttctggt
    37321 gctgtttcag ttcctaaaaa atctgtttgg ttccatactt tcagttcata tggcaataat
    37381 ctgcaaggct cttcagtctc taaaatcgtc aaactactat tgaacaatcc acccatttca
    37441 cgcaactcac ttagctcttt tcctgtcatc cattgaatgg ctgcagttaa agaagctcca
    37501 tgtcctacga ataaaagtgg gccatcttta tgttgggcaa ccgcatctgc tacaacagtt
    37561 tcgatacgtg tgattgccga ttcaatcgtt tctccgtgaa aaggagaagg atcatacaga
    37621 tctaaatgat tacgcaaatt cggcagatta tccggatatt tctggtacat ttcatcgatc
    37681 gactgacctt ctaaagtacc cagccctagt tctcttagct tatctgtata aaaaatctct
    37741 accggacgag caagctcttt gttgatacct tctgctgtat cacgtgctcg ctttgctgta
    37801 ctggaataaa ttgctttaaa aggaacatat tttacctttt tcccaaatgc tttgatttct
    37861 tcgtaacttt gtggcaaaag cggagaatcg ccattgctcc cttgaaaacg tctttcctga
    37921 ttccattgtg tcttcccatg tcgggtaaaa tatagttcca tacgcagcaa actcctgtct
    37981 ttcttttatt ttactctata ataaactgtc taatccagct tgtcattttc atagtgatgc
    38041 gtcaattcta aattagggaa tccttgctgg cgaagtgctt catacactat cattgcagct
    38101 gtattcgata aattcaatga gcggacatgc tcatcattca tagggatgcg taaacacttt
    38161 tcttcgtttt cacgcataaa tgtttctggc aaaccggttg tttcttttcc aaataaaaag
    38221 aagtgctctt ctccatcagc aaaattttcg tcactatata ctttatgggc aaattttgag
    38281 atcaaatgta gtttacgatt acctaaaaat gttaaaaaag cttctaaatt actgtggtaa
    38341 gtaatattta catcattcca ataatccaac cctgcccgtt ttagatgttt gtcatcagtt
    38401 gaaaaaccta gtggctcgat taaatgaagc ggagtattcg tggctgcaca cgtacgggca
    38461 atatttccag tgtttgcagg gattaatggt tcaaataaaa cgatatgatt catcaggatc
    38521 attcctctca tatttatgtt cgtttctatt ttctgacaat ttgatttttg cataaaaaaa
    38581 aacgtatcaa ctcggtgtca gtcactgcga gtcgttacgt tagtgaagct cttctcacta
    38641 acttttatca gaacaggaac ctgataaaaa ccaatgaaaa aacaacttgt tgctaatata
    38701 acaccctcaa cttttttttg caacctctac tcattaaaaa ctgctagaaa aacgttttct
    38761 cttagttcct tttaataatt ctatgtcaat tgtaatcttc ttcaaaggat tcttatataa
    38821 gtctgctttg attcgctctt ctacctgcca ttctaaaggt gacatggcca aaagatcttt
    38881 ttgattttcc tctggtatct caaattcata ggttacacgc tgacgttcag ttaatttcat
    38941 tgcttcagca aacttggcta ctacacgttc attggaataa ctctgttttt caggttgatt
    39001 cggataaaaa gcctgacgta gttcctttaa ataattttct tgtggcacca ccttcaacac
    39061 ttctccatct tctgagagta cacgttggaa ttcttggtaa tgagaaggcg aaaagatatt
    39121 taaaaccgcg ttgaatgttt gatcagcaaa cggcaaattg gtcaaatcag ctacacacca
    39181 aaatgcttcg gcagattgtt ctgtagctgc gtagactcct tcttttgaaa tatcaaatcc
    39241 aacagcattt tgaatctttt tcaattttga aagttcattc aagaagctac cttctccgca
    39301 gccaacatct aatagcgaga cttcttcagg taaatgctgc gcaatagtct caataacggg
    39361 ctgatacatg ccagattgga tcattctacg ccttggaacg aacattcctt gatcgtattc
    39421 tgttttaacc gcatgggata aaaaataaag tgttcctttt ttggaaaggt caaaacggtg
    39481 attatttttg catatcaagc cattatcaga cttttttact tcttcttggc aaaccggaca
    39541 gcggaacagc tgatgatttt ctgccaaaaa ctggcgtcct cggtcaattt tttttaacat
    39601 agttccccct catttctgtt ttatcttacc gttagatgct cactttggca actgctaaaa
    39661 atatgataca atgcctagaa gaaagtgaaa gataaaggag aagattagat gatcaaagaa
    39721 ttttgcgctg aaaactacac atctattcct ctagcaattg ctagaggagc cgatcggatc
    39781 gaattgtgtg acaacttagc ggtcggtggc acaaccccga gtactggtgt gatcgaagaa
    39841 acgttaagtt atgctgggga aaaagatatc cccgtaatga cgattattcg tcctagaggg
    39901 ggcgacttcg tctataacga tatcgaattg aaaatcatgc atactgattt gattgaagca
    39961 aagaaattag ggacggatgg cgtagtattt ggttgtctta ctccttcagg ctggctagac
    40021 gaagaggcct tagaactatt gatcgaaaca gcagaagggt tgcagatcac cttccatatg
    40081 gcttttgaca gtattcctga agatcggcaa ttcgaagcaa tcgattggct ggtagaacat
    40141 ggtgttgacc gtatcttgac tcatggcggt cctgctgaca aaactattgt agaaaatttc
    40201 ccacggctta aagaattgat tgcctataca gatggccgct taatcatttt gcctggtggt
    40261 ggtgtcactg cagagaatgc agcacatgta gctaaagaat tgaacgtaag cgaagtacac
    40321 ggaaccaaaa tcgtggatct tcagttataa tcttataaac aaaaaaacat ttgcaacagg
    40381 cataatgctt attgcaaatg tttttgtttt tttacataaa atagcccata aataaagaag
    40441 caatattgaa ataaatcagt acactcgtca aatcgctcaa agtcgtgata aagggaccgc
    40501 ttgctactgc tggatcaaat cctagacgat ccattagcat cgggattaaa ctacctgcaa
    40561 gattcgccac tgtgattgca aatagcatag ccatccctat gacaaacccc aagataaaat
    40621 tatgtttcca aaaacctacg attagaaaaa tcgtgatccc cgtaacagct cctgtgacaa
    40681 cgccggtcaa gacttcagcc aaaacaagtc gaacaaatcc atgatctttc tcatcagaaa
    40741 tcgcaagtcg acgaacagcc acagctagcg actgggttcc agcattaccg gcagttcctg
    40801 taatcagcga aatgaagact gctaaaatac tcgcttcact taccaagtct tcatagtgac
    40861 tgattaaaga agcggtcgac atacctaaaa acaacagagt gatcagccaa ggcaaacgtc
    40921 gagacgctgc ctttactgga ttttcattaa tttcttccac attgaccccg gcaagtcctg
    40981 aataatcgct agcagcttca tcatcaataa cgtcgatgat atcatcgact gtgacgattc
    41041 ctaacaaatg atcatcataa tcggtaacag gaagagctaa gaagtcataa tctctaaatg
    41101 tttgtgccac atcttcttgg tcgtcgccta catgaactga aagcactcgt tcgctcatca
    41161 gatcagaaat cattgcgtcg tcatcattca cgattaggtc gcgcagagaa ataaccccta
    41221 ctagctgacc ttcttgatta acgacataga tgtaatagat tgtttcagct acatctgctt
    41281 cgttcttcag cacgtacatc gcagagcgaa cagtttgatt ggctacgatc gaaacaaatt
    41341 cagttgtcat gatcgcgcca gccgtttcat cctcgtaatg gagaagttcc ttgatttcac
    41401 tggcatcgtc tgccgacatc aaacttaaat atttagcaat ttgcttttta tccagcgtat
    41461 taagcaagtc aacagcatta tctgtataca tctcagaaag catatcagct gcatagctag
    41521 gacgcatttc atctaggtat tctttcatat gttcgttgtc ttcttcaatc acatcgaaca
    41581 tatccgctaa ttctttaggc gacagatagg aatagatcag ctgcctgttc tcttctgtaa
    41641 gagattggta gaattgcccc tgctcataga tatgcatctc taaaaaactg tttctgaatg
    41701 cctgaatatt attttccctt aaatcttgag tcaactgggc aaatctttcc tctaattctt
    41761 gattttcatc caaaccaatc tcacctcgcc ttattcttca aaccgtctca caactgcctc
    41821 catgtctgcg gctaaaggct gcttgaactc cagcaactct tctgtaaatg gatggtaaaa
    41881 acgcagatca taacaatgaa gggcttgtct ttttattccc agatccattc gcccgccata
    41941 catatcatca ccgagcaggg cacagccaat tgcggaaaaa tgaactctta tttgatgtgt
    42001 ccttcctgta tgcagctgga tatcaactaa cgcgatttct gttagtcgct tctctatcca
    42061 gtactcagtc tgtgccgaac gtccttcatc agagaccatt cgcttcaata gcgaagaata
    42121 atctcgggcg atcggcaaat cgatatgtcc atgtgccgat aaatctgaaa gccttccact
    42181 gactaaggcc tgatattttt tcacaaactt cttttcacga agttgcatat ctaattttgc
    42241 gtgcgcaaat ccatgctttg caaaaagcat caaaccagat gtatcccgat ctagtcgcgt
    42301 caccacatgg attacttggt tttcgtaacc ttgtttttta taataagcct ttacacgatt
    42361 agccattgtc ccatttggat ggtattgggc aggaatagac gaaacgcctg ctggtttatt
    42421 tactaccaga acgtgttcat cttcatacac gatatcgatc ggtgtttcat ccaaaagtac
    42481 ggtttcatgt tctccttcag caggaatcac aatggttacc ttatcatcct tagctagaga
    42541 aaacaagaca ttttctactt gatcattaac tttgatttga ccgccttgaa attttatttt
    42601 cgctaacaga ccctttgaga tccctttttc ttttaaaaaa tattttactt gctgagaatg
    42661 atctttctca taatcccatg taaactgcat atcaatcctc actaataaag gcatctttca
    42721 cacgatgcca aaaatgcata tgtcgataag aagcaaaatg gatccgttct tctgctattc
    42781 gataataaat tgagcggata tttgcttttg cgacattcaa ttgatcaacc gttacaagat
    42841 aatcttctgt attttccaat tttatttcta gccattcatc atgggcaatc acgatcggag
    42901 aacccaaagt acgaaacact cgattattca gtgaagcaat ctctgccaac tgaaaggcat
    42961 tgatactagg atggatcaca gcaccgccga cacttttatt atatgctgtc gaaccagtag
    43021 gcgtagaaat cgtcaatcca tctcctctaa aactttcaaa cagctcgttt ttgatgtata
    43081 catcggctac catcgtacga ttggcccgtt taatcgttga ttcattcagt gctaaaaaat
    43141 gctggtccgg tgtttcatcc aaataactga tccgaacatc cagcaaggga taactaacac
    43201 tttgttcccg gttcgttcgt aagctgtcta ctagttcttc cagctcgtaa tcccgccaat
    43261 ccgtgtaaaa ccctaaatga cctgtatgca cgcccaagaa acgcacatca ttcagacaat
    43321 gactgtagtg atggaacgcc gacaataatg tgccgtctcc gccgactgag atgaccaatt
    43381 ctggctcatg atcatctcgt tgaatccctg cttgttctaa aagtgcgttc aaacgttctg
    43441 tcacttctat agtctttgct tctttattat gaacgatcgc tactttcatc ccctgcctcc
    43501 tctctttcgc tttctttctt caaaagcgtt cgatagtaga catcatctga ttttcctctt
    43561 ccatgggaga ataaccgctg tgcttcttgg atttcttcac ggatactgga catctcttca
    43621 tccaactgat aggcagcttc tgccgcacgc tgaagtcgtt cactcatttc ttctggaaaa
    43681 acgccttgat atttataatt cagcgaatgc tcaatcgttg cccagaaatt catcgcaaga
    43741 gtccgaatct ggatttccgc caaaattttc ttctcaccgc tgatcagctg tacaggatac
    43801 tcgatcacca gatgataaga acggtagcca cttgcttttt tatttgtgat gtaatctcgt
    43861 tcttggagaa ctttcatgtc ttttctttta cggatgatct caacaacttg atggatatct
    43921 tctacaaact gacacatgat ccttaacccg gcaatatcag acatttcttc ttctaaacgt
    43981 cccattggaa tatgccgtaa ttgtgatttt gttacaatac tatcgactgg cttgactctc
    44041 ccagtgacaa attcaatcgg gacatgtcga ttttgttcac ggaactgttt gcggatcccc
    44101 cgcagtttca ctttcaattc tgatacagtt tgttcatatg gtgctaaaaa tgcttcccaa
    44161 tctcgttcca tagctgatac ctctacttca ttaacattca ttgtttattc taccatatat
    44221 gcaaagtttt ttcgtactct tgatttatgc attgaccaaa aaaagaaaat taggcaaaat
    44281 agacaaaaag gagtcgatca aatgacacaa atgcttgaaa tcgaatataa atctatgctg
    44341 acaaagcaag agtacgagat attcatcgct cactatcaat taacagataa agatttccgt
    44401 gtacagacta atatttattt cgataccgtt gatgaacagc tgaagaaaca aaattgtggc
    44461 ctacggatcc gtttcgttgg tcatcaagct gaatatacat taaaaacacc tgctgaaaaa
    44521 gggcgtctcg aaacaacgga tacctttata acagaagaag cagaagcatt tatcttcgaa
    44581 aaacgtctgc ctcgttcagg cgcagttttt caaaaacttt ctgatttgaa tatcgatcct
    44641 gcttcattaa tccaaaccgg aaagcttaca acaaaacggg ctgaattccc tattgaagaa
    44701 ggacttttag ctattgatga aagctgggga gacaatctac acgattttga actggaacta
    44761 gaggtaaggg gatgcatctg tgggaaaaat tgcttttata aattttttaa aacgtttttc
    44821 tttaccttat cgtcctgcta agaacaagat ccaacgtatg cttgaagcta aaaattagaa
    44881 acctttcatt ttcacaaggc acttgagttc atttttttac ttttttctgt taagttatag
    44941 gatgtgaaag aatctagtca ctagttgtat agaatctatt ttgggggaga ttcatatgat
    45001 cgaaatctat ttatttgtta atcctttagg ggaatactgt ttcgaaatgg aacaacagtt
    45061 attacaattt atcgaagaag agtacgggac accatcaaaa gaaaagatgc aatttcgctt
    45121 tcttcctttg gtcaatttgc agacaattgg ggatgtcatg caacaaaaag gaatttctca
    45181 gaacaatctt gaggaacgta atcacttatt ctcaatgact tattctgcag cattggactg
    45241 caaagcggcg cagtttcaag gaaagaaaaa aggccgccgt tttttgatca aattgcaaga
    45301 agcagtcggc tgtaatgaca taccatattc tcaagaactt gctgaatcca tatttgaaga
    45361 aatcggcggc gatttgaata tgtttaaaga agatcgtcag tctgacttcg ttaaagaagt
    45421 attcttttct gatcaaaaaa ttgctcggga aatgggcatc acaaaacacc cttctgcagt
    45481 cgtttacaat tatgcctgcg accgtgattt tggtgtttta gtagaagtaa aaggtacaag
    45541 tagcctttgg agaatcacag agctgtgtaa aacaacagat aactcatatc aagtttttaa
    45601 tggtgaacat ttacatacaa atgattctca gttccattca agaactagcc atttacactt
    45661 gctttctaat taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt
    45721 ttctattcga gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt
    45781 catttagacg ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat
    45841 caactcctgc ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag
    45901 ccaagtagtt ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag
    45961 cagcagaaaa gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc
    46021 gtgaccattc gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat
    46081 tcaatttacc ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt
    46141 ccgtatgcat gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa
    46201 acccatccaa gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca
    46261 ataaatactc cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg
    46321 agtagtcgcc gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc
    46381 ccatttcgtg gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat
    46441 atggatttgt gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga
    46501 caacatcgat ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca
    46561 ttggtttcag tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag
    46621 cttcccctaa gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac
    46681 gtttccgtaa ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca
    46741 aagtatcgta tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc
    46801 tgtaattacg gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag
    46861 caaatgtatt tctaaattgt tcgtaaacac tgtacaatcc cttaaatgct gcttcgcgca
    46921 cccttcgatc cgtgctttct agcaactgac catacacgcc atgagataat tggactattt
    46981 caccattttc tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg
    47041 tatcacttga agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag
    47101 ataagacatg ggctcgatta tctactattt gctggatata atggcgatag acttccaatt
    47161 ttggttcttc tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt
    47221 caaaccagga aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg
    47281 cttggtaatc tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga
    47341 cttcagtttg gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc
    47401 cttgatcaag tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat
    47461 atttctcatc aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt
    47521 caggcaattc ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc
    47581 ccttagctct atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac
    47641 ttttctactc attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac
    47701 atagaaaacc gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt
    47761 atgcattcgt taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat
    47821 tcccttttat gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt
    47881 tccaaggctt cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa
    47941 aaccgacttt tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag
    48001 cattgttctt gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat
    48061 ttcttcccat cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta
    48121 ctagtgaata tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa
    48181 atatgccctg atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga
    48241 tcagctttcc agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc
    48301 aatgagaatt gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg
    48361 ttattcaaat aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg
    48421 atttctattg ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag
    48481 gcttccaaaa atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac
    48541 tgtaagtgct cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg
    48601 gcaaaatgag cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga
    48661 caataatatt tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc
    48721 attgcaagca gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga
    48781 aaaaagaggg cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata
    48841 gttcccaata tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat
    48901 ttatacgaaa aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt
    48961 tatacaaaat aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat
    49021 tcattcaatg tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc
    49081 tgattctcta catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag
    49141 cgctcttcga ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt
    49201 tggatcactt gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg
    49261 gcgcttccag ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca
    49321 ctcacatctt ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga
    49381 tttttactga taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct
    49441 tcgcttcctc ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt
    49501 tcgatttctt tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct
    49561 tcatgaccaa ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta
    49621 cacctcactt tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta
    49681 aagaaaaaaa atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc
    49741 aagctatctt atttaactat aacatttttg gcagacgaat actatatagc cgacttttca
    49801 gctaattttc cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc
    49861 taatcaagaa tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc
    49921 ttt
//
LOCUS       DANKAY010000011        45551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000011 DANKAY010000000
VERSION     DANKAY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            99..284
                     /locus_tag="PWY98_000617"
     CDS             99..284
                     /locus_tag="PWY98_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL2036393.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            351..746
                     /locus_tag="PWY98_000618"
     CDS             351..746
                     /locus_tag="PWY98_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2036394.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            736..1113
                     /locus_tag="PWY98_000619"
     CDS             736..1113
                     /locus_tag="PWY98_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036395.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            1135..1509
                     /locus_tag="PWY98_000620"
     CDS             1135..1509
                     /locus_tag="PWY98_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036396.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(1816..2214)
                     /locus_tag="PWY98_000621"
     CDS             complement(1816..2214)
                     /locus_tag="PWY98_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2036397.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2406..3173)
                     /locus_tag="PWY98_000622"
     CDS             complement(2406..3173)
                     /locus_tag="PWY98_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2036398.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(3208..4431)
                     /locus_tag="PWY98_000623"
     CDS             complement(3208..4431)
                     /locus_tag="PWY98_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2036399.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            5412..6215
                     /locus_tag="PWY98_000624"
     CDS             5412..6215
                     /locus_tag="PWY98_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2036400.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            6800..7255
                     /locus_tag="PWY98_000625"
     CDS             6800..7255
                     /locus_tag="PWY98_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036401.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            7374..8432
                     /locus_tag="PWY98_000626"
     CDS             7374..8432
                     /locus_tag="PWY98_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL2036402.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            8474..10423
                     /locus_tag="PWY98_000627"
     CDS             8474..10423
                     /locus_tag="PWY98_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2036403.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            10426..14067
                     /locus_tag="PWY98_000628"
     CDS             10426..14067
                     /locus_tag="PWY98_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036404.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            14467..14805
                     /locus_tag="PWY98_000629"
     CDS             14467..14805
                     /locus_tag="PWY98_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036405.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            14915..15136
                     /locus_tag="PWY98_000630"
     CDS             14915..15136
                     /locus_tag="PWY98_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036406.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            15156..17312
                     /locus_tag="PWY98_000631"
     CDS             15156..17312
                     /locus_tag="PWY98_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036407.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            17324..17557
                     /locus_tag="PWY98_000632"
     CDS             17324..17557
                     /locus_tag="PWY98_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2036408.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            17547..18413
                     /locus_tag="PWY98_000633"
     CDS             17547..18413
                     /locus_tag="PWY98_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036409.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            18428..20398
                     /locus_tag="PWY98_000634"
     CDS             18428..20398
                     /locus_tag="PWY98_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036410.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            20565..20792
                     /locus_tag="PWY98_000635"
     CDS             20565..20792
                     /locus_tag="PWY98_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036411.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            20789..21283
                     /locus_tag="PWY98_000636"
     CDS             20789..21283
                     /locus_tag="PWY98_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036412.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            21458..21793
                     /locus_tag="PWY98_000637"
     CDS             21458..21793
                     /locus_tag="PWY98_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036413.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(22050..23633)
                     /locus_tag="PWY98_000638"
     CDS             complement(22050..23633)
                     /locus_tag="PWY98_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036414.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            23755..24339
                     /locus_tag="PWY98_000639"
     CDS             23755..24339
                     /locus_tag="PWY98_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036415.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            24350..24595
                     /locus_tag="PWY98_000640"
     CDS             24350..24595
                     /locus_tag="PWY98_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036416.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            24601..25323
                     /locus_tag="PWY98_000641"
     CDS             24601..25323
                     /locus_tag="PWY98_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036417.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            25454..26197
                     /locus_tag="PWY98_000642"
     CDS             25454..26197
                     /locus_tag="PWY98_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036418.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            26203..26568
                     /locus_tag="PWY98_000643"
     CDS             26203..26568
                     /locus_tag="PWY98_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036419.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            26584..27114
                     /locus_tag="PWY98_000644"
     CDS             26584..27114
                     /locus_tag="PWY98_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036420.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            27276..27644
                     /locus_tag="PWY98_000645"
     CDS             27276..27644
                     /locus_tag="PWY98_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036421.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            27835..28095
                     /locus_tag="PWY98_000646"
     CDS             27835..28095
                     /locus_tag="PWY98_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036422.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            28713..29513
                     /locus_tag="PWY98_000647"
     CDS             28713..29513
                     /locus_tag="PWY98_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036423.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            29550..29732
                     /locus_tag="PWY98_000648"
     CDS             29550..29732
                     /locus_tag="PWY98_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036424.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            29922..30209
                     /locus_tag="PWY98_000649"
     CDS             29922..30209
                     /locus_tag="PWY98_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036425.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            30300..30539
                     /locus_tag="PWY98_000650"
     CDS             30300..30539
                     /locus_tag="PWY98_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036426.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            30667..30996
                     /locus_tag="PWY98_000651"
     CDS             30667..30996
                     /locus_tag="PWY98_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036427.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            31049..31880
                     /locus_tag="PWY98_000652"
                     /pseudo
     CDS             31049..31880
                     /locus_tag="PWY98_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31895..32212
                     /locus_tag="PWY98_000653"
     CDS             31895..32212
                     /locus_tag="PWY98_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036428.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            32215..32447
                     /locus_tag="PWY98_000654"
                     /pseudo
     CDS             32215..32447
                     /locus_tag="PWY98_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32562..33038)
                     /locus_tag="PWY98_000655"
     CDS             complement(32562..33038)
                     /locus_tag="PWY98_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036429.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            33164..34495
                     /locus_tag="PWY98_000656"
     CDS             33164..34495
                     /locus_tag="PWY98_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2036430.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            34520..35047
                     /locus_tag="PWY98_000657"
     CDS             34520..35047
                     /locus_tag="PWY98_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036431.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            35034..35597
                     /locus_tag="PWY98_000658"
     CDS             35034..35597
                     /locus_tag="PWY98_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036432.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            36015..36752
                     /locus_tag="PWY98_000659"
     CDS             36015..36752
                     /locus_tag="PWY98_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036433.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(36769..37185)
                     /locus_tag="PWY98_000660"
     CDS             complement(36769..37185)
                     /locus_tag="PWY98_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036434.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            37578..38825
                     /locus_tag="PWY98_000661"
     CDS             37578..38825
                     /locus_tag="PWY98_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2036435.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            39095..41485
                     /locus_tag="PWY98_000662"
     CDS             39095..41485
                     /locus_tag="PWY98_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDL2036436.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            41534..43369
                     /locus_tag="PWY98_000663"
     CDS             41534..43369
                     /locus_tag="PWY98_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2036437.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            43438..44301
                     /locus_tag="PWY98_000664"
     CDS             43438..44301
                     /locus_tag="PWY98_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2036438.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            44291..45370
                     /locus_tag="PWY98_000665"
     CDS             44291..45370
                     /locus_tag="PWY98_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2036439.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT        16800 a         6430 c         8692 g        13629 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggttaaaa catcctcttt ttctattgat tccatacaca
       61 acaatgtgta taatagattt tgtaaaggga gggtagctat gcctaagaca gcaagagaaa
      121 ttgtaaaaat tctcaaatct aacggcttca aaaagactgg acaaactggc agtcatgcaa
      181 aattttataa ttccaagact aaaagaacgg tcgttgttcc gatccacaat ggtgatgtac
      241 cactaggaac agaagctaat atctggaagc aagcagggct ttaacagcct tgccttccta
      301 tttggtatta ggcatactat cctctcttac actagaaaag gagcgaggaa atgaagacaa
      361 aaaatatttt ttatccggca atcttcgaga aagaaggaga agcttataac atcgttttcc
      421 ctgacttacc ggaaatatct acctttggaa atagtcttga agaagcatat ttaaacgcta
      481 aggatgcttt aggattagcg ctatatagtg taccagatgc agaatttcct aaaccatcag
      541 cagtggaaga attaactttg aaagccaacc aagtggtagt aatcatccaa ctaaacatta
      601 aacttttccg aagaagctta aatacaaaaa caattagaaa aaatgttagt gttccggaat
      661 ggttagttct tttagggaag gagaaaaaca taaacttctc tcaactactt caaaaagcac
      721 tagaagaaga gttgcttgaa aagtaaaaag gagaccgaac ctatgaaagc tacacactta
      781 aactatttct ctcgaacgtt aatcaataat ctattaaaag atacaactgg cattagcgtt
      841 agtaccaaac actttgccaa tgatctaatt atttttgagg ataataactt gcgaattgag
      901 atgtttattg aacaggcaac ccctaactca atacctgaat tctgctatac gcacactgaa
      961 aaaatgaaac atgcaaaatc agttaaagat gttgaagcat taagtgaatc actaacgaac
     1021 gctttaaacc tcgttaagaa ggcaaaattg ttatttgaat ttcaaaccga ctaccacaat
     1081 gagttgcttg ctcaatctga aattttagat taattgaaac aggagaagag aaagatgaaa
     1141 caaacacaaa cagaaaccgt taacgatacg cttgctgatc aattagcagg ttattataaa
     1201 ttatttgaga tgggtaaagc acagcgcttg atccactatc tttggaaata caaaattttg
     1261 tttctttttt gggaatcaac actaacattc tcagttcttt tttataagtc agatttttat
     1321 aatcaacact tagttttagg gagttttttg cttatctaca ccattttgat gcttaattta
     1381 tatacctaca tcacacttga agaacactta gtaattagcg atgcagggat cgagagaaac
     1441 atcaaaaggg tatccttcaa gatcacgtta aacaagccta ccgataaaaa gctctcagaa
     1501 gcgttctgag agcttttgac attttcttgt ataattttat cctaacacat tacaaagcac
     1561 ctaattcgag cttatatcaa gcaaatttgc tatttattac tcaatacata tctaaaaatt
     1621 acttgatgga aaattagata catggtaagc atgaaaagaa aatcaatttg agcagatgca
     1681 gtacaataca tagcaattta aaagctctca gaaggcttct gagagctttt aatatattct
     1741 ttgataattt gtctttctta aatccatacg tcctaaaaca agctgttttt gcttaatatg
     1801 atcatctgag agacatcaaa tcaacttttc tctcaacaaa tcattcaaag ctttcgacag
     1861 attaatattc tcttcagccg ctaaaacatt taaatagtgt tccacagtca ctgattttcg
     1921 atcataattt tgcttgtttc gagctttagc aactttcgta ttagctttta cgacaaataa
     1981 attaacattc tcagatcgct tgattcttgc atttaactct tgaacaggcg taggcgatgg
     2041 aatttctttt tcttgcaact ctaaaagcac caagtatcct tccaatactt cttgcgccat
     2101 ctcaaaagca tcttctaaag tttcaccatg tgtatataag tctggaaaat ctgggaattt
     2161 aactgcatat tcatttcctt tccattcgaa tactgcacaa aaaaagtatt tcatcgcatt
     2221 gcacctctct aattaaaaaa taatttttgc ttttttgttt caaaaaaaag aatcgcctac
     2281 caaaaaaatt gataggcgat cacaagctaa ttaacctcta aaggagatta ataccttgct
     2341 cccaaggcac tatatatctt agcatacgtc tgaagcatct tcaaacgagt aagatatatt
     2401 ttgcattaac ctaatttttt ttctctataa gttttttcat caaactcact agaaatatta
     2461 aactggacag caaaattgta atttctgcct tttctaactt tagcaaaatc aatattcagc
     2521 attgattttt cattaatttc ctcaactgat tcccttaaaa cacgtctatc aaaattcgat
     2581 attgttttat actcattttc tgttttcgta atctcacgaa ttttaactat ttcaatcagg
     2641 ggattcttat atatttcagc tttctcaaaa tctcctttat ttttggcgat aagatatttt
     2701 ttataataca tcgccaaata tttgtataac acgatgctat attttttttg aaaagatgct
     2761 aagtcttcaa aaacaaagaa cggatacttc tttttgattg agacaattaa gggaattaca
     2821 tcttctgaaa aactgaaaga tacactatgc tcttctaaat cccaatgaat ccgatcaata
     2881 gcagatacag tagtatacgt cttatatttt ttgtctagat tcgaaatttc ataaactgct
     2941 tgtctttgca aagtgttaac tatggttttt agttccgttt caagattttg atcaacatcc
     3001 atgtaattaa taatcgcttt aacaggtaaa tctactacgc ctgttttctt tagctttgtg
     3061 ggtgagatat aactgaacag caattgataa agttcatacg actttaaatc cattttcatg
     3121 tcaattgttg ctaagaactc ctcattgtac tttgtcagtt catctttttt caaaagtttt
     3181 tccttccttc acgcattttt tcagttgtta atcataaagg ttcacataag gcaaataact
     3241 cgtaattgat ttttcgagat atttaacagt attttttatt gtgtaatcct ccttatgagt
     3301 agcaacgtaa gataggtgat tgacaactga ttcaaatcct ctatctttgg ataaacgatc
     3361 ataaagcgga taaactttat tctgaagtcg gctcataagt ttaagatcgc ataagtcggc
     3421 agaactgatc aaaaagtttt tgattagtaa tttagtgtat ttgctattca tagctttcaa
     3481 gtacgattga ttcattttct ctgttttatc ttctgcgcct tttaaataag cagggtctct
     3541 ttgttcttgc ttataaggta ccggaactga catggtacgt gtaagcaaga actttatgtt
     3601 tgtataagta tgacccttct tgataagctc atagtcaatt ttaaaatcag tgtatttatt
     3661 cagttgggca atcggttctt taattacacg tctttcaaag tctcgaaaag ctttatattt
     3721 tttctcaata ctaaatttct ttcgtaaatc tgcaatggag aaaacaggat tttttaatct
     3781 atcaagtgat tttgtactcc atgttaagtt atctctattt gcttcataca gattaaaatc
     3841 tttagtgaat aattgaaaaa aactgatcgc atacttgctt gtcaagttaa ctacttgatc
     3901 aaatgtatat tgcgtaaaat gattttctaa gttcgttaat aaaggcataa ttgaactact
     3961 aaatttaata gatactgcat cagaaaaatc attgaattca gtaatttcta gcggtgagat
     4021 gactttgtat tcagtcttac cgttaggttt agttctttga attttaatga cagattgctg
     4081 atgcaatttt tcaataactt gttttaatct tgaatgtttt gaagaagatg aactagaaga
     4141 catcaacgca aataaagcct ttttagatac tttgacggta ttattaaaag gcggattttt
     4201 agggtcaaga tgtcctatag cgtaaagata aattttttgt ggcatagcat ccatttcaac
     4261 aatgctatct acaagttgat tcgataaagt aacagttaaa tcaaactttt ttagattgct
     4321 ttcggtagtt tcaaatttcg ggtttacatt cttttctgtt attttcatac gattgcctct
     4381 tctttctaag cttggtacga gcttaaaaac ttggaactgg tacgattcca ttgaataagt
     4441 atagacctgt tttcgatacg ttacaataag aaatgtatcg taaatttata acataactca
     4501 atgtcagtaa aacacatttt attttttcca atcaaatttc attgaaaata cagcagaaat
     4561 cgctatttta gaacattttc aaaataaaaa tccttttcaa taaaatacga aattataaaa
     4621 attaccaaaa ttaaaaaaca aatatttttc tagtttttag aaataaattc acatactttt
     4681 tctttttttg gatatattcg gaataccaca tcgctttttg tccacgtttg catcgctttt
     4741 tgtccacatt cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc
     4801 tatttaagat tgatataaag cctttttgga ggttgctaaa gttttaaaga ttaaaatata
     4861 tattataaat ataaataaaa ggggaagctg aagcttcctc cttttccttt ttttcaatta
     4921 ttcaaagtta cacaagcaaa ataaactcct agatagtgtt agtttttttt aagatttttt
     4981 atttttaaga ttttgaaata agagaggagt aatcctcctt tattaaatat ctgttaatat
     5041 atctgttaaa tatctgttaa tatatctgtt aggggaggct taatctcttg ggagagtaag
     5101 tattaaaggg gtataaaggt aacacttttg gacagtaaag gtaacaaaag tggacagtaa
     5161 gggtaacagt tttggacagc aaaggtaaca aaagtggaca tttgaggtat cacactagga
     5221 taagacgtta ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa ttagattagt
     5281 aacaaatctt tgttgactgt tacctttagt tgtgatttaa tttaggtaac aagcagaaaa
     5341 aattgtttcc tttaattagt accagattaa catgattaaa ggagcgcaaa aagaagattg
     5401 gagagtaatc aatgtctcaa gaagttaccg aaaagattat tgatagtgat gtagatttag
     5461 tatcagaaca aacaaatgca attgtgcgtt ataacaatgg tctgaataca acagctttgc
     5521 gaggatttaa gcctgttgaa atggatgtgt tttgggctat tttatcaaaa atgaaacgtc
     5581 aaggaacagc agaagtaact tttgatttca acctctttcg ttatttggtc aaatacgaca
     5641 gaagagaaaa ggacagtttt tattctgtgt taaaagattt gagtgacaaa ctaggtaatt
     5701 taacctaccg ttttgaagat tcagacgtct atgaacagct gtggcttttt caaaaattcc
     5761 ggatagataa acagaaggaa acagttacaa ttaaggcaaa tgaggaattt gaatttatct
     5821 taaactcaat tggcaaaaac tttactcgtt ttgaacttga taattttaca aaactaagaa
     5881 gtagctacac aaaagaattg tatcgtcatc tcatgcaata cagaaataaa gagtggcgaa
     5941 atggttattg ggtagtcaaa gttgaagatt ttagaagagc attaagtata ccttcaagtt
     6001 atagaatgac aaatattgat aaaagaatac tagagcaggc gaaagaagaa tttttagctc
     6061 ctgatgaaaa cggaatacag gtatttgaaa cttttgatta tgaaaaaatc tatgcaaaga
     6121 aaggaagaag agttgatcgg ctcagattca cttttagtga gccagaatca aacttgccaa
     6181 ccatttcaat gcataattgg ttagaagaag attgagaaga agcaggttgt aattttaata
     6241 caacctgctt ctttaaaaaa gtatgggtaa cacttttgga caataaatgc aagaagtttt
     6301 tttgtaagca agtttcagtc taaataatat ttgaaagaat ggctaatcta tacaaatttt
     6361 aacaactatg ggtaacactt ttggacaagt caagaaaaaa aacaattttt cttaatttgt
     6421 tgatcgacta aaggtaacac ttttggacaa cacagaataa aaagtccggt gatatcagtt
     6481 ttggtaggaa acatcttaat atctttgtga gattgaaggt aacatttttg gacaagtcaa
     6541 ctatttgtta aaagttaata tagagtaatt agataagttt tttcttttcg tctgataaat
     6601 ggataatatt agttatttta ttgctaatta gtctttcatg atgactgaaa tagctaaaat
     6661 aaatatctcg ttggataatt gctgatactt taagggatat ctaaatttgc tgattgattt
     6721 atttatgtta ttatgatatt ataataacat aaatgtgtta cgcccgaacc tcattttaaa
     6781 aaaatgaagg ggtgagacaa tgaatgctat taattggcat gaatttgtta actatatgga
     6841 gagcttacta gatgatctaa aaagttttag tattgctgat gcagtaaagc atgggttacc
     6901 agttagtaca acgatgaaat tacgaggatt gaaaattcca gatgaaatta ttaagctgag
     6961 aactaaaaca attattgaaa ccgcaaaagt cgttgacaat atgaaccatg aactaaaagg
     7021 caagaaaaca acttacgaga aagattttga acgtttttta aaatctgtta ttggaaaata
     7081 tctaggtaaa aaatctttca gtgaattgag agatgaagag attgtaccta ctccaatggt
     7141 tagcaaaatc cgaaaaaaca aagccgaggg gaattactat cttggtacaa tagcaaaaat
     7201 cattgattat ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa gataattttt
     7261 gtgttaaata ttaatgggga agaatatacc gaaatgattc aaaatcccga acctagaatt
     7321 accgaaaaaa cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa ctaatggaaa
     7381 aaaacgtcaa ttcaacatct accaaagatc aatacagtat tattggaggg ttgcttggtg
     7441 ataaggataa gagaacacgt cacggattac gcattatgct tgcaactttt ctaggagtgc
     7501 tttttttatt aacaggcttg ggtagctaca tttatcaatt aggaaactat aattattatc
     7561 tcgtgaacaa tacaacacct ataggctctt cattggagtt tgctcgatca ggagcagaga
     7621 tcactatttc agatgtgtgg acagatcaaa acagagatgt aacagtagta aaactcggtt
     7681 actcgcaaaa cgctagaaag ttgttatcta cgcaaggaaa aaactacaac ttatatatgg
     7741 caacgaaaga aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg ttaagtactg
     7801 atggggatgc tttcttattc ctaaaaggta aattagatga aagagcttat caagttttta
     7861 ttgcaaacca agtatcacta atttctggtg atgatacagc tgacgatgat accaaagtca
     7921 atgtgaatga agcagattca gttacaaaag cactttctca atattctatg aatgatgtta
     7981 ataatgaagg agtaatttct tttgggaaaa aagcaaaacc tgttaatact cctgataata
     8041 tcaatttcag aataaatcca tattcagatt caactaatgt ctatcaagga agctttctta
     8101 ccaaagatgg cgatattgat tattcaaaag ttgtagcagt tacaagtaag aataatgttg
     8161 taagtaagtt agaagatcag ttaaatgctt caaatactaa catcaagaaa attgaagcta
     8221 caaaagctga gtatctggaa cgtttacaac aaaatcctga cgataaagat tcgcaagaaa
     8281 gtattcgtca actagacagt cgaatggaga ccgaaattaa agcaatcaat gatacacaaa
     8341 aattgattga tcaatacaaa aatgctgact ttaacaaaga ttcttttgga gacatgaaga
     8401 cgacatttaa gtacctaact ctgactgact agaagtaaat tattcttatt ttagaggaag
     8461 tgaacacaaa cagatggaaa atagtaccga gttttttaag aatattcaaa ctgaatttaa
     8521 tcatgctctc acaccaatga aaccaccgaa acataagtta gtgggacgtg atgacgaact
     8581 ccatgagttg tcactgatct tgaatagacc ggaaacaccg caagctatct tgttaggtga
     8641 agccggaaca ggtaaaacag cacttgcaag tgaatgggct gaaagacagc gacaagatgg
     8701 aaagaaagta caagtataca aattatcaat cggacaattg cgtgttgggg gagatgatgt
     8761 cctccaaaca cgtttagaaa cattaattcc aagattgaaa gaatatgagc gaattttaaa
     8821 atctgaggac aaagaaaatg aatttgtcct cttcattgac gagtttcata aagttgttag
     8881 ctcttttggt agtggcaata aaattggggg tgattcgcta aaagaaccgt tagcaaatga
     8941 atcacttcaa ttgattaccg ctactacaag aaaagaatat gaaaactata tcaaaagaga
     9001 tgatgcctta gggagacggt tacaaccgtt agaattgaat gaactaaaaa gcgatgttgt
     9061 cgtcgatatt ttgagacaat ggctaaatac atcctctgtt aaagatgatc ctcaggcttc
     9121 ttttaaatta tcgcaaagga ttcctgatga attacttcaa gaaatcgtac aagcaaacaa
     9181 gctttatcgt gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg aggatttgat
     9241 tgcaaaagat cgagtagacg gaaatgctta taatcgagaa agtctattgc aaatatttgg
     9301 taagtataat attcagctag attctgattt tgatatggaa aaacttcaag aggaattaaa
     9361 gtccataaaa gggcaaccgt tagcagttta tactataaca atgttgttta aacgtatgaa
     9421 gcttggattt gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc tgtttacagg
     9481 atcaacaggg gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac tttatggcga
     9541 tgaaaataaa gttctcttag ttaatatgcc ggactacaaa acaaaagatg atgcaatgcg
     9601 ttttagacga ctcataggta ctcatgtaag gcatgatcct tctgcgatta ttgtgtgtga
     9661 tgaatatgag aaagctggtg tagaaacact cgatgccttt ttatcaattt tagatgaagg
     9721 taggataagt tttactgaaa gaggatggga tgatcgagaa gttcacgaca ctgtttcatt
     9781 gtcaaataca attttatttg cgacaacaaa tgcaggtgcg gagatgttcg attcgttaaa
     9841 cagatatagc tcactatctc gtgttacaaa caaggaaaat tctaatgaat atagcactga
     9901 actaaaaaga gaataccgaa aagtcgcatc agggatcaaa caagccttga ttggtgaagg
     9961 tctaaagcct gaagttatta accgatttgg acgaatagtt ccattttggg ctttatccga
    10021 acaaactctt ctggaaattg cttataatga aatgttgaag agtgctgaat tagtacgaaa
    10081 acatttaaat atagatttac gtcttccacc taggaaagaa agagtatatg gtcaaaattt
    10141 ttatgcagac gatctctcca tgcacttagt ttttgacatg gcttcacaag aggattctaa
    10201 atcagggggt gcaaggaata tcttgcattt agttgaatct gagtttgaag aagcgattac
    10261 tgaagcaata attacttatc caaacgcaaa aatattcgat gttaagttga gtgaagccgg
    10321 agcgtttgaa ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt ccgttgctga
    10381 aagtaaagaa aacaagattg tattttcgat tggaggggcg tagctatgaa attcaagaaa
    10441 tatttactac aaaaccttag aataaaacga tgggtcgtaa gaggtctagt tattggagga
    10501 gtattagcag ttactttagg cgctcctttt ggaatgaatg atccaactcc tgacaatcaa
    10561 gatagcaaac aagaaacagt tataaataag aacgttgcat atgcggaaag taaaccggca
    10621 gaacaaggaa tgtggacact atatgctcgt atcctaatgg gagataaaga agttaatgat
    10681 agtgccgcta aaactggaca aatgaatttg ttaggacaag gtggtatcag tgccgatttc
    10741 ccttattctg acatttctaa acaaggtaat tcgattgcag ggggtaatgc aggtactcaa
    10801 ctagcatcta ctttagcaac ttatagttac tacggatata ttgaaacagt ctcaggaaat
    10861 gctattgcca gtcaagcatc atccattcta ggaagtattg gtaggacgat tggtagtttt
    10921 atcggatcaa ttagtttaat cctttctaat attgctgaat ccataaatga actccttgga
    10981 aaatttttca tgagtatgaa tatattttct cttatggggt ggggagaagg tggatcggat
    11041 gtatctaatc cggtatctaa agcattaact agcttgctta acaatttggg attaaataat
    11101 gaattattta aagcgatagc aagcttatct atgctctttt tcacagcaat actaggttat
    11161 caaggaatga aattattgtc gaaagatggt gttaactcga agggattatc accagtactt
    11221 gaaaaatgga ttgttcgtat ttttgtattg tttatattct taccagtggc aggatcgcta
    11281 ttgggagttt atatcaacgg catccagtca gcaaaaaaag atggagatat accaacaaat
    11341 gttgtttctg actacatcat taacacaaga ctttgggctt ctactcaaaa tcttgctcca
    11401 tcatctgata ctggtggtga tgtacctgac ggaaagccta catccgaaat caagttcgtt
    11461 gatacagatt atgaaccatc taaaaaatct gcaatgataa gagcgatcaa tactagcagt
    11521 tatgccagac aaaataatga tctagattct aattcagtcg gtatggctct attaaaagaa
    11581 tggtcatcga atgatacttt caatattaat acttatgccg gtgatgttaa tgtcgccgaa
    11641 aatgatgctt atatgggaag agcgcctgct gtttggacag gctataattt aggggatgga
    11701 aaagaaggaa gtgcaattaa gaaaccaacc aatttaaatg gatatatttg gtcagcaaat
    11761 caaaatgtga cagacgacaa tcgcaagcct gatggaaaag gaacagcgca taactttgat
    11821 gctactcaac cttggggcgt tgctaacaat gcaactttta gcacacagtc ggtagcttta
    11881 ttgctccaat cggcacttga tacatcggga gctaagtttt atgcgtataa tatcgctcct
    11941 tcaggaattc aaggcagtgc taagaatatg tcaacagtaa aaacagaatg gcgtacagca
    12001 tccttagttg gtcatggagc tatgggtaag tttggctcgt ggctagcgat gaacgctgaa
    12061 tcaatcgttt tagcagttct ttacttagca tgtgcttggg cgatgttcaa tgtgaatatc
    12121 gtgaaagcat ttaaacgtga atttattcgg atttgggaag tagttctaac aggatcactt
    12181 gcatcaggag caggtgtgat ggcaatttca ttaggtgttg ttacaacatc tctgatcgga
    12241 ctacaattac ctatctttct gattactttt attcgtgcat ttggtgatac tgctgtggga
    12301 ttcattccta atacattagt tggcgatggc gtagcaaata ttgttcgatc tgctatgatt
    12361 ttaggtgtag gatatctttt agtctttaaa aaaggcgaaa aagtaaataa tttgattaat
    12421 atgcttattg gcttaccact tgaagtagcg tttgggtttg atgaacgtgc taagttatta
    12481 tttggtaata gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc aatcaagaaa
    12541 agccaaggag ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa agatatggtt
    12601 gcaggaggtc ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa caatatggga
    12661 gatagcaatg ctgatgctaa gtccattgct gataaagtaa ttcctgaaac accagaagga
    12721 atgtcattca acggtggcgg agttggttcc acaatcaata atacctcttt caaagattta
    12781 gttcgtcaag atcaactacg taataactcc caacaatata ttgatcattt aggacgtatg
    12841 gttgatgaaa acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga tagtaaatac
    12901 ggtaaagatg ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga tgaagatgga
    12961 aaccttatag atactaatgg taaaccattg gaagatagtg aagatggtaa tattggtatg
    13021 gatagcgaag gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt
    13081 gttgacgaaa atggacgcag gttgctaaat ggtgattttg gtcatgatag taacggacgt
    13141 ttagttgacg aaaaaggtcg tttagttgat gaagatggaa atcttgttga tgagtttggt
    13201 aataaacttg gtaaacacag cgatggatat aatgtgaatg gagatttagt cgatgaggat
    13261 gggaatatca ttgagacgag agatgcgctt gtggcagatc gaaatagaga actcgaacaa
    13321 caacggcata cgccaattgc tgttgcaatt cctgagaatg aagaagacga gattacttta
    13381 gatcaaactc atcaagaact gaggaatgaa atgagtggtg aggacgtgtc agacagagtg
    13441 catcgggaat taggagatgg agcatctggt gaaagtcagt caagagagct taacagcaac
    13501 tttgataata gtgctattcg gaatgacaca gtacttaaag acagagaatc gctaacaaaa
    13561 gaaacggata aagaacaatc agatgtgaag aaaaagttca ttcctactcc taatgttaat
    13621 caaacagata aatatgaaac aaatattgga tcaggaatgt cgaaagacaa tcaatcaatt
    13681 agagaacctg aactgacaaa taaaaataat agaacaaata attctaaatc tacttcagat
    13741 aattcaggga aatctaatca atcaaccaag aaaacatcaa cagctaaatc agccgttgaa
    13801 tatctatcaa aaactggtaa aaatgtttac acctcagcag ataaagcttt agcaaaagct
    13861 ccaaaaggta gtctagcagg gataacctat gaagctaaaa acgaagctat taaaataggt
    13921 aaaggaacgg ctaaggttgc caaggctact gctacaggag catccaaaac tggtaaagta
    13981 gtgaagaaat ctaccatgtc agtcaaggac tatattgtaa aatacggtaa tcaaaaatca
    14041 actgaaaata agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc cttcttttta
    14101 ctttgatttc taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa aattatttta
    14161 aattgtctat aaatgaatta aatatttact gttcaaagtt gtataattaa aaagggctta
    14221 tgctggtaaa gaacagaatt attatctaaa caaaaacttg ataaaccagc atttttagcc
    14281 aataaaaaaa gtgaaataat ttaatggaaa atctctttta atcatcgaaa atttatgtta
    14341 ttatgataac ataaagatgt aaatatcgag acgtcttttt cttagttaga tcattgacaa
    14401 gtgaacaaat aagatttgtc gatgatataa ttaacttatt aaaaatatta aaaaggaggt
    14461 ttttatatgt tcgattctat taaaaatttg gttagcggaa tgacgcctga tatgaaaact
    14521 ttagtacaag ttgttttgta tttcttggca ttcgtttttt cgttgaaacc aggattcact
    14581 gcaatgaaat tttttggtga tgcaaaatgg ggagatggat taaaatatgt tggggcgatt
    14641 attgcaatct ttattattcc tgtaattaca accgttatgc tttacacaat gggtactaaa
    14701 acaggtaatg atttgaacca aaaagctaat atcatatcag cacttattcc agttgttggt
    14761 atctatctag tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg aattgaacaa
    14821 atttggataa tatgccgatt tgttcaattt actttttacc aaaaaactac gctccaagcg
    14881 tagtttagaa gtgagggaag aaagtaaatg taagatggtt tttgatagag aaaaaatgtt
    14941 agctcacgcc aacgaagtat taatgagcag tctgaaaggt accgaactgg caaagataat
    15001 gaatatgaat gttaaccagt tctatgatta tcgaaatggg agtaaaaaga ttgaaaaagc
    15061 cagacttgaa acgcttatta aattcgaaaa agcatatgta tatatgctag ataaacagaa
    15121 aagaactatt gactgaaaaa aggagtttta caataatggc aaaattcaaa gatattaccg
    15181 aatctattaa ttatgtggca gaaacagttc ctgtagttgc actctctttg aagccacaat
    15241 tactcgaagt gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct aaagcaaagg
    15301 tatggaaaga attcgatgcg ttgctgacca aagatttacc aaagaaaaat tatatggtaa
    15361 ttgaagatgt acaaaagctc aacagtgagg gagttataga tgaggatgat gctattctcg
    15421 attctgagaa gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct gatctaccag
    15481 aaaaatttaa agagaagcta ttagcaggtg tccgttttat ggatagtagt ggtcaattat
    15541 tagttattcc ggatgatcct aatgtgtttg aatcagaagt tgaagaagct acagacttct
    15601 atagcgcagt agagcaatta gaagtagcta ctaatggatt agaacgaatt gctgagacct
    15661 tcaactcttc tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca gatgaaaaag
    15721 aagctctttt tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa gtaagcatag
    15781 aagaaacaca aatacaaaca gaaacctacc aacaaccaaa aattgatgaa aaacccgaat
    15841 cccaaagtga caaagaaaat tatgtttatg aagctgaaaa aatcacatct gaaagtgatg
    15901 taccgaaaaa cttagatgct gattttttag aaaaaaattt tgaacagatc attgctgaca
    15961 aatttcaacc tgtagtttta gatgagatta atgaccctgt cgatttaaac gaggagatgc
    16021 tatccatacc tgagttgaaa ccattgctgt ttttagctaa agagagtatg aacgctcgca
    16081 tctctattgc taataataaa atcaacagtg aattaaataa gacgaaggag caattgatcc
    16141 tcaaaggaaa gcaaatgtta gctgatgagc ttttgcggat agaggaagca acctcattaa
    16201 atggtcaaaa tcagtttaca aatacactat cggttgctaa agagagctac gaagcagaag
    16261 catctaaaat tgatgattta gtgatggaaa aatccaatat cattcgtaaa gattatgata
    16321 tggcgaaaca ggcttatttg gatgctgaac taaagaaact agagcaacgg tatgatgctg
    16381 agcatttacc agaagttaaa caacaagaag atgaatatag aaaagcaact gaagaaagtc
    16441 ttgatcaaca gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg aaacaaagcc
    16501 aagaagcaaa agaagttgtg gtggaacgaa ttatcaattc attgaaacca gaaattgaaa
    16561 aatcattaac agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca aagtcagaaa
    16621 atgaagctga gttccatgat acaaaagaga aaacaacaga tgtggttgtc aaatattttg
    16681 cgaacaaaga agaagttgaa aagcaggtag ctaacaacac gaaagaatta gaaacaaaac
    16741 ttgtagagca acaaaaaact atcgaagaat ataaagatcg aatgaaagag gtcaaagatt
    16801 tagcagaggg ttataaatta aatagcgaaa agttggcaga acaaagtgaa gctttaagag
    16861 aagacttatc tcgttcccga agtgatctag ctcaaatggc agaaaaaacg aaatcattag
    16921 cagttgctac agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa aagatggcta
    16981 ctatcatttc tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc tactttatta
    17041 gtcataataa tcaacaagca caggctcaaa ctcaaacatc tatttcgaga gtggaaaatt
    17101 cgattgggac aaatacgaac tctgcttcaa caaatagtag ctatgaagca acaccaacta
    17161 cagagttcaa agttgggaaa gatgttgtca tcagtattga tgggaaaaca actaaggctg
    17221 aagtgatttc tattgatgga gaaaaagcag ttgtgaaaac tccagacgga aatcagtaca
    17281 aaattcaagc tcacttatct gataataact aaggagggct attatgcgag aagaaaaaat
    17341 agtttatcca gtaattcaaa acaatcaaga aaacatgacc tcttctcagc acttacagga
    17401 agcacagctc gtgtatcagg taaatgaact accacaaacg aacgagacaa tgagcaagtc
    17461 aatatcttta attggcttgc ttattgttgc aatagtgata actgtaggtg tttaccgtaa
    17521 gttgaacgtg aaaggaagta tttctgatgg cagataactc aaactatgga tttaaacata
    17581 acttacatca aaaaccttct gaaacaattg tcagagttga atggcttgaa aagcatgtca
    17641 atagcaccta ttctgttaca gcaaaaagat taattgtatc agctatagtt gttgcagtcg
    17701 ttgttcttgg atcaattgtt tacttagttt ctgatacgat taaactgaaa tttatttttg
    17761 ttccaacgtt attaatgata attggatttc ttattctaat tagccagtat atgtttattg
    17821 atccatctag tggttcattg atttctggtt ctattagact gttcaaatca gtagttaatt
    17881 cagcaagagt atctatgggg aaaaagaaac catttcgagg cacagagatt ttaaaagtga
    17941 aacaagatgg aacaatcgta tatcgagatg ggagtgtagc attaatgttc aatctagatg
    18001 gggctacatc actaaccgct tttccagaag aagttatggc tcaggaatcg aaatcaatac
    18061 aatatcaaaa taccagagat cgttccttag ctgaaatcaa aatcacttca tctcaacctc
    18121 agaatacgga aagacaaact cgaaatatga aaatacaaaa acgaaatgca aggagtaaca
    18181 aagcagtctt tgacataata gatcagcaag aacgatttgt atcagaatat attgatggtg
    18241 tgaaaacaac agtaatccaa tatcttatct tgaaatctca atctaagatg gtattggatg
    18301 aaggtgtgga acgtttagaa aactatacga gtagaggact ttattatagt gtcactcagt
    18361 tgtcagctaa acaaacaaaa gaaatattat ctgatattta tgcactgaaa tagaggtgag
    18421 agattaaatg gaaattatgg gtagatcaca actattaaat ttcaaaaaaa agagaaaaac
    18481 taaaaaagaa caaaaaacta gaaattcaaa ggtagtgaat aatacctttg ttagtgattt
    18541 aacatcttta gttggttatc aatttacacc atcttacatt aggacaggta agcggtgggg
    18601 aacaattgtg aaattagtga atacttatgg aatgaataga aattatggat ttggttggat
    18661 ggttaaagct attcctgaag tgagagtaga aggggtaaaa gcctatctaa ttgaagcaga
    18721 taagccagtg tcacaaaaag atcaacaaga aataatgcac gagaatgttg ggggatcaac
    18781 gaatagttat gaaaataatt tagaaggaaa aaattcagat gctgaaatta gcaaaactgt
    18841 tatgcggatt atggatttga atacagcatc tttactagac agcatggcaa ataaaattat
    18901 tgattggaag ctagaaatcc tattagtatc cgattctcct aataaaatca gcgaacagct
    18961 aattaaactc caacggttgt atgatgaaaa aatgtatgga ttaaagctta tgtcatatgg
    19021 aggagatcaa cgtgatctgt ttgatagact tttaagtgaa ccaatcggga atgtctatga
    19081 gtatacgtct atgagttcgg attttgcagg aaatgatcat gcaattcgca aaggtttaga
    19141 tgatgcgaat ggatgggcta tcggcactat gtctactagc tacgcaagtg gtgtagcttt
    19201 tatggcactg gatgattctt ttacaagtaa agttttagtg gcatcacaca aaaacgctac
    19261 aattaaaggc tatgatgaaa aaatctcagc atcatcactt tgggggcaat tgattgctaa
    19321 taatgcaatg gcaaatcata aacgaacttt tcatattgtc ttaaataaat ttgattactt
    19381 tggtgatcca actggtagaa catttgttgc caaaccttcg ttaaacagtg taatagaaaa
    19441 ggttaatttg gctgaagaag gtcttaactc tttggagctt tttggtaatc aaaaggacat
    19501 tggtgattta tatagacgga atctcgataa actaactcag attttttatt tgttagcagg
    19561 cagaacatta gattctgcaa ctagagtttt attgaaaaaa gaattaggta acttttatat
    19621 aagtcagaaa ctttgggata ttaattatga agaacgttca aaacttttgg tgaacagtag
    19681 tgatacaatg ccagttcttg gggattttat tagctatttg actaatgctg aaacaaaagc
    19741 caaaaaatct ggtactgaaa aagaagtcga tcgtattatt gtattaaaag acacattgag
    19801 aacagcatta gacagcttct ctaggatgtt taatcaaaga acgacactga aagaaatcgg
    19861 taaaagtgac aaattacagt tttactataa cttaggtgat ctaagagcag aaaccaatgt
    19921 tatggaagct caatttttaa atacctttga tttcatcaca tcacaagcaa aaaaagatga
    19981 tattgttatg attcacggct tcgataagtt atctattgaa accgttgggc taattaaaaa
    20041 aagattaaca atgcttgaag aagatggaat tcgtcttgcc tttctatttg atacaatcgg
    20101 ttcaggagaa gacaatgaat acaaagaaga tattgaaaca gctgatatat tcaatacaga
    20161 tggaaaaatt taccaaagtt ttgaaagtga ttttaattac actatcattg gaaagatgac
    20221 cagtggagag ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag gagaagatga
    20281 tcaaaaactt tctgttgatt tgagacagta tttgacaaca ggaagaagta ataaatttca
    20341 gatcaggaga ccagtggatc tgactaataa ttttgttatt ggagagttta ttctttaagt
    20401 tttatctttg attttgagtg tataaggaaa ggataagaaa aaacatgtat atttgagact
    20461 aaaaacagtc ttagagaaca tggtacgttt tgtgaaaaga gaactataga aatttcataa
    20521 agtgaaacct tattgagtga aataattata gtggaggtta aataatgaaa ccaaacataa
    20581 aaattttaga tcgaatattt ctaggtagag acacagaagt aatcctaatt caacatgaag
    20641 aaggatttga agttagtata ggtattcaga aacttcaaaa acctcattat tgtaatcagt
    20701 tgtataaaaa ctttactgat gaagaaaaag cgagagtttt ttttaatgaa gtcaaaggta
    20761 tgagagaaca gtatgaggtg gttgaagcat gaataagtta ccagaaaaga accgtgtatt
    20821 tagattgaaa ggtgatctca gtcaatttaa aatagatgag aatggacaat tttttgtttg
    20881 gatgcgacat gagcattgtt ggtgtaatcc ttttgtgtta aaaaaagaaa atgaaatcag
    20941 aactaaaatt gcaaatggtg agtgggatta cctcttcaga tgggaagatg aatcttttat
    21001 agcaaatgat atggatcatg catttgtctc atttctggat aatacatggg gtacttggta
    21061 cgattgttac ctagaaaaat tgggctttca aaatattgat attgatgcta ttgaatgtct
    21121 taaagaagtc gatcaaaagt atgaagaaga accctattat ctcttgcaac aaaaattagc
    21181 caataattca gtgtttcagg tagaaatttg ggaaaatgaa gatggaactt atgaggggct
    21241 agcgcaagaa atacccttct cagataggaa gagttcgatt tgaaaagagt atatcagcaa
    21301 tttttatgaa gtgaaagcaa gcttacaata tagtaatgga aaattgtggt tagctggaag
    21361 gtatattcca gctaaccaaa tcttacgttt tctgggagag tggcgtttta aaacaatttc
    21421 ctaggaaaaa tagttaacat aaaaggaatg aattgttatg aaacaatatt taaatgaatg
    21481 gaaagcgata gaggggtcat tagtaacgca aagaataaag gaattacctg attgcttaga
    21541 gaaggatcat ttgtttcaaa tacgagaaat gttgaaaaaa gaacagtttg atccagacca
    21601 atttttagta gtcgagtatc caactattgg agtgtactgt tgtaaccata tcaatgatgg
    21661 aaaatatttt attattcagg agtatggagg acagttaact cctttctata ctacatggga
    21721 aatgagtgaa gaaggaatca ataattttcc atgtgaaagc atcgaagatt caatttcaca
    21781 caccgaatgt taattgatta taactataaa aaaggattga acattatgtt caatcctttt
    21841 ttcaatgaaa tgttttccgc catttcattg tttggtgcga tttctatttt attatatcaa
    21901 aataaagaga gattctaaag aaagaagagg gaaatatata aatcaagcga cagtatattt
    21961 attattaaat gctattgtga gaatataact aattagttta atttgaaagt aaatatacgc
    22021 tataaaagtg catcttgtgt tataaaaaat tattttattt tgtgaacata atttaacaat
    22081 tctttctcgg aattgatttc ttttcttaaa tagtcttcag acttattaat aatattatct
    22141 attatgtcta gatacatatc acttttcgat tcagctaatt catacagttt ttttaattct
    22201 ttatccttta attgaaaacc ctgatccata gtcactgcat ttggatatag aagcccacta
    22261 taatgtttac gtaaattcac ttggaatttc tgattataaa agtctaacag attagaaata
    22321 aaaggcggaa aattactgtt tttgtaaaat tgattatttc catttaggtt gtatagtaat
    22381 ttgttaaatt gctcctgatt taatttattg taaatccatg gtaatacttc catatcctta
    22441 tctgctaacc tgaatgaact agcatcggta agatgttcga tcataaaatc tatgaacatc
    22501 tctttttcac caaaatataa atattgatta tatattgttt ctaatgttga agatggtagc
    22561 gaagaaactc tcattttttg ctggagtata ggatcaacta gactatttaa gtgattttgt
    22621 aattcttcat tgttgtttcc tagaactgca agggaacgta aacggtagta atattctatt
    22681 tctgttagta cttgttgttt cggtttaaaa tcatttattg atatttgtaa attttgaatt
    22741 gtctcgtctg aaaaatcatg ttgtaattct ggatgggtag ctataaaagt aagtaaaaag
    22801 aacccttttg tatacgtgat ctgttgattt tcaccaagtg atttattaat ttcctctctt
    22861 gattcagctg tgaacctata ctttgcaatt tcttttttta gaatatttaa atagaattct
    22921 ttattatcaa aaattagggc attaagtagt ctgaaagtac tttccctatt tctatctgct
    22981 tcctcatttc cataagataa ttgaaaaaca aacttaaagg ctgtcttaca taggttttct
    23041 ttggcttttt cgcttagttt atttagatag ctatttttta gtgcagagtg aaattcttca
    23101 ggtgtaacag acatagcatg tatgcgactt gcatattgta taatttcgat cgttactttt
    23161 tttcctaata ttggattttt taggaatata atttcatagg acttccgtat aacatcctga
    23221 caagtttctt ttgttatatc tcttaattcc caagtattac cgttgtaagt gactacagga
    23281 tgagctgaat aatttctttg atccttaata tattttaaat cctcatattc atagctatca
    23341 attagctcag ttgaactata agctttttct agtagttcca tttcccattt tggagagttt
    23401 ggatcacttt ttcttagtct agctatatct tctaaaattt tcttagaagt attatcctcg
    23461 taaatatttg ctaaatcaga taatttatca attaaatcta aaataagcgc actgtaattg
    23521 acagctattg cagaacgata atttccctga taaaaactac ttctaacttc ttctaataat
    23581 ggaataattc taggattttt aacttttttt atttcatcat ctaatataaa catataaatc
    23641 tccttttttt atttgtatga caagtttacc ataaaaaaaa attaaaaagc gagacatatt
    23701 tatgttgttg tgttatcata ataacataaa tatgtagagt gagatgatga aaaaatggat
    23761 ttgaaacaat cgttttacat ctggttatat cctgataatc ctatgtcaat aatggctgat
    23821 atttttgtag ttagcatctt attgtattta ttttatctaa ttttctttca caatttaatc
    23881 gggtacatga ttcttgattg gaaaggaatg ttcgatatag aaagaaaacc atttgctaac
    23941 aatcaaacct ataccgtctt cgaaagtgga gattgggaag aaaatgaagt tgatgtgatc
    24001 catgaaattg gattagcttt atcaggaata caagatgctg atgttttggt atcaatgaca
    24061 acggatactg gatacaacgc ttatgaggta ttttatctta tgtattttat atggcgtgat
    24121 ggtcaagaaa ttatcaatgg tcgtgctgaa aaaacaatca attctaatac tgaaatttct
    24181 ttttgtatgg gtgaagggga aatgattgta tataaagatg ggtcagcaaa cattaaaaca
    24241 gcctttggtg atattgaaac aagtattaag gaacttaggg aatatgtgca gaacccatat
    24301 aattttcaat atttagcagg aagaataaat ttagtttagg agtaaaaata tgaaaaaaat
    24361 tataaatcaa ccaacaaatc acttgataat tgaaggtgtt attttcgtaa caatgtattt
    24421 tactgtagga aatggcatct caaatacaat cattcaaaaa aatccacaat acctattatt
    24481 tagtctttat gcttggattg tacaaatggt ttgggtttcc atattgatgc tgttgtttta
    24541 ttggatagta cgggttattc ttttattgtt aaacaaaaga aaaaaggata ggtgaataaa
    24601 atgaaatcaa ctaaattgaa ggttgtattg ttatcgacag cagcattaat aactcttaca
    24661 gcatgtggta caactaatag tgctgataat aacaaagaca caaagtctag tagtacggtg
    24721 gttactaaaa acagctctac atcggcatca acaacaattt cttcaatgac tgaaaataat
    24781 gctgaaaagt atcaaaaata cgaagacatt aataaaggtc aaaagccgga agaagtttat
    24841 accgacttta ttaatacagt aaaaaatgat gaagattatg ggaatgatcc attgcaaaat
    24901 aaaaatctga ctatcgttct ttacaaacct gattgtccag attgtcaaaa agcagaatca
    24961 aaaatcatgc aagaaataaa agaaggattt agttatgctc caacattaat gcatgatatt
    25021 accataaata aaccagctta cgagtatcac gcagttgctg tagatattac tgatggtgta
    25081 cctgagtgga tacaaaacat tcctggtctg gctgatgaga acaatcaata tcatacgcca
    25141 acagttgcag tagtaacacc aaagaaagtt aatggcacac tatactggga tgtaatggga
    25201 atgacaacaa atacaactga tgaaatgatt gaaagtacaa ctaaatatta tatggatttg
    25261 ggagcatata aattatatca acagtcagaa cctgttatgg acgatcaatc caaagttaaa
    25321 tagttcttta gggcgtaagc cctatttttt taaaattaag tgataacaaa taaatgattt
    25381 ctgaaacaaa cgttcttact caactactcg aaacacacaa gctaattaac tagattataa
    25441 aggagaatgc gtaatgaaag aaatcagtgc aaatgaagct catattccgc aaaagaatca
    25501 ggttggtata tataaggtgc ttcatggtga aggaatttat aaaatacagt tacttaccgg
    25561 ttacaataac ggtgcaaatg ggaagtggtt aaaggcaaaa agtattcgtg aacttaaaac
    25621 tgaagtagaa aaaataagac ttgggttaac aagggaagaa ataagtagac tgagtgaaaa
    25681 ttcttctttt gtaatccaaa aagaaaagga gaaacatcta cttcatcggg aactaataat
    25741 tgaaaaatac tacgatgttg atttacctga tcagttaaga attattgacg aattttcaga
    25801 gaaaaagtgg aaatctgaaa tatacccaag cttaattcaa tacaaggcat caggaaagcc
    25861 catgtataac tacacaggct ttttaaacta cacacaatat aaatatgcct ttcgcaaatt
    25921 aaaaggcatt aaacaagctg acattgccct gtatgagatg aaaaaaatgg atagattgaa
    25981 attgattcaa gagttagtgc aaaatacatg gaatcaagcg attgtcgaac tagaaaaaga
    26041 aaatgggaaa attttaacta atgaatatct gatcgtaaat gaaattgaag aattaattgg
    26101 tttattttca tgtgaaaatg gtgaagtctt cttgaagtca tcaccaatct attcaatttt
    26161 ggacagtccg tatcagttta tacaggtaag gagatgagtt acatgtctag cttgaaagat
    26221 gactatatcc aagcaaaaca agagtatgaa aaaatacaga tgttaaaatc tgagaaagtc
    26281 gaattatttt taacacaaaa attagcagag gatcatattg ctaacttaga tgctaaaact
    26341 ttagaggaat atgatcagca gtttaatcag acacatcaca atatcatttt gcttgttgat
    26401 caggcaaaga gaagacttga aaaagcggaa tatgaactat tagatcaagg aattatatgc
    26461 ttggataaag ggattcgaag atattatagt gcagagttaa aaaagcaaaa tgtagacaga
    26521 catgcgttaa aacatgaaat cttagaattg ataatgcaac gaatttgaag ggatagaaag
    26581 taaatgtcac aaaacattag tgctgtaaaa caattaatag aaaaagattt agagacgaca
    26641 gttcagaggt tagccgaacg aatccatttt gcaaattatc aaaccatctc taattggtca
    26701 agcaggaaca gaaatccgga agaaattcca atgaaatttt tttatcgaat cggaaaagaa
    26761 tatggtgttg atccgataaa aattttcgaa aagaccatga tttattacta tcatggaaca
    26821 cctaacgaag tgttggaaca tcttttggaa gaagcagaag aaaaatcatc aatattagag
    26881 atatcacaaa atgacaaagc tattcaagtg aaatgtgttg atccaacagg aaagattatt
    26941 gttggatttg atattgaaag aaaatctaaa atagatttta ttactcaatt aacaaagata
    27001 aataatttaa atattttaac acttagacca gatcatgaac agttgtttat gagttttaaa
    27061 aaacaattga aagaaataca aaaaagatat aacaaggtgt tttctaatgc gtgaaaagct
    27121 aaaagaaatc agagtgtttt ggataattaa caaagaattt attatcttgt atacacttgg
    27181 atttatcagc atgatcttgt tttggtggtt atatttaaaa aagtaattta aaagtgtcaa
    27241 ggagattttt atccagagaa ggatgtggaa tttgcatgtg gaataataaa ttaaaaacag
    27301 gcttgttatt aatagttatt tcctgcgcta tgatggttgg catgataata caaagggaac
    27361 aatcttattt tgaagtttct gctaacaatg taatagaaag atgccattat ggtcaacatt
    27421 tttggactgg ggaagtcaga gaaaatatag atcgtgagta tgttcaaaga atcgtttggg
    27481 atgcatattc tatcaaagat tatcctaaaa gtttaacgag tagattattt tacagcgaaa
    27541 aagataatca aaaactctcg aatttgatga tgaaaaaagt aaggaaactt gcacaaaact
    27601 atttggaaga aaaagaaggt aatagtccaa ttgacaaaaa ttgatgacac tgcaaaaaaa
    27661 tatagagttc gaattagctt ttttcggagc attgtttttt aggtgaacta tatttcatca
    27721 ctaaaatcag agataactaa cttgaaacat ggtcagatgt gttacttgaa gtctctgaat
    27781 aatcaatgtt atctagtaga attaagcaac taaaaaagaa agaagtaaga aaatatggca
    27841 tacgaaaatt tacaattagt agtcgcagaa gttagtgggg atatatacct tacaaaagta
    27901 aaagatggaa ttatggacac gaattatagg agagttgcta cagatgatgt gctaagctca
    27961 tcgacagaat ggtttcgaaa aaatgaagaa tctgctgttc actttgaagg aattgatgga
    28021 aacattcata gtttgttcta tacaaaagac aaagaaaaag caaaaaaaat tcttgatatt
    28081 ttgaaaggaa ggtagttaat taaccaatga tataagagaa tgtgagacca agtaaatact
    28141 tggtctctat aattttagcc actagtcaat taaatagcta aacaatttta ataataataa
    28201 aatatgactg cttcataatt aagaagtgca gaatttttct aagaacgata tttccttgtt
    28261 gaaatgagta ttgctaacaa tttgatcacg aacagcatcg ttacaaatgt tgtatcaata
    28321 tttgatagat tacatattct attaaaaaag aaaagaaaat tattgatatt atacacatta
    28381 gtgtgtataa taaaagagag ggagtgatta gaactttgag caattcacta attgtttaat
    28441 aatagaacga ttcctaagaa gttcattgtt cattggcaaa ttgctttatc agattgaaag
    28501 ttggcgaata aggtttgtag aataacacct tttcaagcaa agcgattttt gaaagctatg
    28561 aaatttgttt aataaaaaaa ttcaccagat aatctgatag agactaaccc aattactaac
    28621 agttgtaatt ggtgttagct tacaaattaa ttattgtgtt tagtcaatta caaattgtga
    28681 aacggcacta ataattggaa aggaaaaatg aaatgtctaa agatttacga gagcttaaat
    28741 tgcgaaaaaa gcaatataat caatattttt ctagtttgtt gaataaacat attgaagaag
    28801 gacttgatca tgattttgga aaagatgaat attgtttata ttgcgaacgt tacaatgaaa
    28861 aaatgtctga atatctacag ctgaaaaaag agatacagaa attagagaaa aaagtacatg
    28921 cttggaagta taaagattcc aggtctggtt atgttgaatc tcaaaataag ccaagaaaaa
    28981 agaagcaagc gtatgttcta aagatggtaa atggggaaga gttaattgta ttagcgtaca
    29041 gtgaagaaga ggttcgagaa ctcgttgaaa cacctgtcag gaaaaaggtt gaaacaataa
    29101 atgagataga cgacagccaa tacaatagca tctatattga aacaagtcat ggtggatata
    29161 agaaattagc aactttcctt agaagttcta aagttgggat aatttctaaa aaagacataa
    29221 gtgctgttac cgatagtttt tgtactttat ctgttcctaa agcttatttc caatacgata
    29281 gtaaaagatt tgaacgctta gagaaattct gtagaccgta tactgtattg aaaactaaaa
    29341 aatttaataa aactaaaaga gtttcgatac agttaccgac tggtcaaatc tcattaggtg
    29401 aaaaagacta tctgataaaa gtaggaaaag agttaattat aaaagtaccg gaacacttat
    29461 ttaaagaaaa ttttgagata cgtgaaacct tggtatggaa agaagaaaaa tagtttggaa
    29521 gacatagaat ggtataaggg gaaattgaaa tggcagtaaa taaaatattg caggtattaa
    29581 tcatagacga atttggcgga ggagaatggt ttgatgtgca gtccatagac tttgaagaag
    29641 aagtcgtagt tatagaaaat atttacggcg aagtatactc atattcgctt gaaggtgaga
    29701 atgttgagga tctacggttg attggtgagt agaaaaaagc agaaatatca aaatggagat
    29761 aaaaattaat agcatgtaaa gatagtcatg cttacatgct ttatccaaaa tggtatgcag
    29821 acaaaaatat agctgaaata aacgattatc taactagtat aaaggaacgc aatgcaatta
    29881 attaacaata gacgaaaaat gaaattaaag gagataggaa aatggtagaa gttgaatttg
    29941 gatcaaaaga atggtttgaa gagttaaact caatgattaa gttcccagaa aagtttactt
    30001 tttactacaa taaaagagac aatatcattc aagtagcttt acttgaaact aagggaagat
    30061 tagaaaatac tcgatggagt atgataaaac catcggcact tagatttcat cctaaagact
    30121 tgacagcggt tgtagcggta aaagaggttc aagactcaca agaatattat gaagtagtaa
    30181 aaaatctgaa ggatatttgg tgtaaataat tctaaattga ccagttattt ggtcaaaaat
    30241 agtatgtgat tatttcatcc aatgtggcat cataattggt atggaagggg ataaattatt
    30301 tgaaagaaat agaaggtatt aagaaaagaa atcaggaaag attggaactg ttaaatgaaa
    30361 atagaaatag aatgtttagt tattatgaat ctatgaggtg tttaaataaa tctagaagtg
    30421 aaattataga atcatcaaag tataaacaat tgatggaaga gagagtttct ctcatccaag
    30481 aaatagaaac agagtctatt tttccagatc attctcctaa agtccgattt tataattaaa
    30541 cattaacaaa tgaaacgatg ctaaataggc atcgtttttt atctatttat aaaattaatt
    30601 tatttacaaa atgtgtatcg gtatgtatgg tgaatttgta gataagttga gaaagggtga
    30661 tcttaaatga aattagaagg tagcttacaa aatagaatga tggaaggcag tacaacaaac
    30721 aaggagattg tagttggaat gggagcaacc gagcttctat ataccgacag aaatccttat
    30781 acagtaattg aagtaaaaag caaaaacaga attttggttc aagcggatgg agcaatcaat
    30841 aaagctactt ttccagatca agagtgggaa tatagcagaa atcctgaagg tcaaatattg
    30901 gagttagtta agacaaaaaa tggatggaaa gtgcttaaag aagatattca tttttatata
    30961 ggtgatagag aaaaatacta cgatcctagt ttttaaacaa cctgaagatg agcgaggttt
    31021 tatcctcgct cagaaaggag tatggaggat gttcagtcaa gaagaaatca tcagcaaatc
    31081 gcaaccggat aaaaatggaa ctataatcat gaagaaaaaa atcccttgct atcgttgtag
    31141 cggatcaggt cagtacaagt ttatgtctat ggggaaaatt acctatggca catgttttaa
    31201 gtgcgatggt gatggatttc ttgttgtaac tgaaaaatcc tttacgccag aatatagaac
    31261 taagctaaat gttttaaata aaaaaagagc tgatcgaaaa ataaggatcg caagagagaa
    31321 agaatctcag aaaaattttg atcgttatgg ctttgaaaag ggttatattt acgtggttat
    31381 agagaaaaat agctatacga tcaaagatga actgcatcat caaggagcta cttatgatcc
    31441 tttattaggt tggttttttt aaggaaaaac aagaacaata caaaactcaa aaagtttttg
    31501 tagaagaatt ttctgtaatc aatgatgaag gaattattga gttgaagcta gaagaataca
    31561 gcaaaagaca acaggagatt ttaagtgcga agcgtagaga agagggtgag tttattggta
    31621 caatcgctca acgattggaa ttggatttga aacttaccaa taccgtcacc atagatacac
    31681 aatggggcaa tgtttacctg catttatttg aagatcaaaa ccataattca ttgatttgga
    31741 agacaaataa atttctttat tatgatgaat gggatgttgg agaaataaga aaagttaagg
    31801 cgacaataaa agcgcatgac tactacagag ataccaagca gacttggatt aaaaatgtta
    31861 aaattcaaaa aaatgagtaa gaagggataa aagtatgaaa agagaagaat acaaacaaag
    31921 attaaacgaa ctgcttgaag aggacgaaac attaactcat ggttcgccgg atgaaattct
    31981 atacatgatt gataatatgg taatttttgg tggatatgaa cttggtaata ggtcagttga
    32041 tcataatatt ttagaatttg atgatgtatc atgggaagaa attcttgatt ggggaatttt
    32101 ggcagtacct gaaactaaaa cctatattag tgacactatg gttccttttt ttgaggaatt
    32161 ggattataaa agacttccca aaaatgaaaa tcatatactg ggaggaaact aacaatgtta
    32221 agtgacttac ttattttact gatctatcta ggtggaactg aaattattgc gtattttttg
    32281 cttgtaattg gtgaatatgg tatttccagt gcaatcactc aaaggtttag attaccagta
    32341 agtgaactaa aaaaatgatg agattatcag catatctttt aatgtttttg aatgtggtca
    32401 tgttaatctt tgtagtaatc ggtgtgactt tcaatttaac ggaatagagg gaaatctata
    32461 atggaaaaaa gagaaaagaa tgaacatatt ttttgagtca atattgttga tggtttttgc
    32521 gatcagcgct taccagaaat ctaaaaaaga gtaatgactg tttagtcatc actctttttt
    32581 tgtagggaaa ttgaaatatg tggagcttgt attggtttag ttaggattgc ttggctataa
    32641 ggagctagtt ctttatcaaa accatcaaag taggttttca gtagcgattt aactgatcgc
    32701 tcatcttgtt tgatttggat aatttcagca tccaaatcgg ttgtgaggtc aggagctttt
    32761 gaagcgatta cctgattttt tttatcttcc aactttctta ggctgtttaa caagttgtaa
    32821 aaataatcat tttgactgtt cattccaacg tgagatgcaa ttttaacaga agtttcatcc
    32881 gtattggcat caagtatttc atcaaaagaa ctcattggaa tatcaagtag taacgaggat
    32941 gtttccttta cagcattttt agtttttcca tcttttttgc tcttaacttg agatattttt
    33001 ggatctagtt taggtaaatc acttgtttta tcagtcattt attagctttt cctttctatt
    33061 aatttatgtt attatattat cataataaca taaaaaagaa cactttttgg gggcgtagtt
    33121 attgaaaaaa tctaagagaa agattgccac gttgcttacg ttaatggtgc agtttacagg
    33181 gattgcaatg agtgtaatca caataaatcc gcaagtgtta catgctgatg ggatagctac
    33241 ttatgatgca caatttaact gtactactga aggtagtggg tcaggaacag atagcgatgg
    33301 aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc aaaaatattg aagatattta
    33361 caaatacatg aatggagaat atggcttatc aggtgctagt attgcaggta ttttaggtaa
    33421 ctggaaacaa gaatcaggta ttgatcctcg aacagtagaa gggtattacg gcgatgggca
    33481 gagagaagtt gacttcatca aacaatttac tgatggtcta atgggtattg gctatggtca
    33541 atggacagcc gggcgacata cagcattagt tgattggtct aagaaaaata atggcggttc
    33601 ttggtggact acagaagccc aattggactt tatgttcaaa ggtgatgggg gattcgtagg
    33661 aatattaaaa gattatgctt taaatgcttc tgatgatgta gtagaaaatg cagtaacttt
    33721 tcataaaaac tgggaaattt cagctgattc cgaatcagct gtaatagctg tacgtggtga
    33781 aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt atggacggtg caaaagatga
    33841 aagtaagatc aataaaatat ccggaggttc aagtaccgga gatggatcga ataattcatc
    33901 aagttcagca tcaggaacag tagaagatgc ctgtgcaact acggatgatg ctaattctgg
    33961 ttctggatca attgggaaag aaggaaaaat taacggtaaa aaaggtcaag taatagcaaa
    34021 tatgacttat gaagaagcat taaaaaaata tgggaaagca atcacgctac caaaatttaa
    34081 agaaccagac tggtcaacat caccttttca aggtgaatta caaggacaat gtactgaatt
    34141 aacatgggct tatatgagtc aattatatga tggaaatcaa ccaactttgg gaaatggcgg
    34201 ttacgtttgg gaaagctaca aatcggcagg agcaaaggtg acgaacaaac caattgtagg
    34261 ttatgcgttt agtgctttag atggatggtt atgggctgtt ggatcagcag gacatacagg
    34321 tgtagttata gcggtgtttg acgatggatc atttttgtgc gctaatttca atgttccacc
    34381 tacccaagca ccaacaagag gtgtttcagt aactttgatt gatggtgtgg atgggtcaga
    34441 taatgtgcat ttctttagcg gtgtaggcaa agctaaattt gaaattgaaa aataaaaatt
    34501 tgaatggagg gcgacagaga tgaatagtaa aacgaaagat gcaattatca tttctttgct
    34561 ttctggtatg ctattagcaa tgcttgttgg aattctatat ttcaataagc agaatcaaga
    34621 catagaagct gaaaagcaca gtgtgcaaat tgaaaatgaa ttgaccttct ttaaagaggt
    34681 taaaccaaat gaagtggatg gattgcaaag agatttagaa aatgatattc aagagatgtt
    34741 taaaggatca gaccgaagca aatttgcgaa tcagtcaggt gaagaatttt tgcgaaagtt
    34801 atttattgtg acaggtaagc caccagttac taacgagagt agcgagcaag aaaaaataga
    34861 atactataaa ccttttactt ttaagctaac aaactttgga attaagacat ctagtagtgg
    34921 gttgtatgaa gcattgactg atttcgaagt tcaatacaac ggaaaaaaag tgtatgatac
    34981 cacattaacc atcacactaa ataatgataa gaaagtgact gggggtgctt tttatgcaaa
    35041 atcatgaaat tactactgct aaaaaggcga tcggaatact ttccataatt ttgatccttt
    35101 gttgtgtggt actatttcaa aagaaaaatg cttatgataa taatgtcaaa gaattggaag
    35161 accttaaaag taaacaaatc gaagtagtca agaataacca gaaagtgaaa gctatgcaag
    35221 aagcccaata caaagaattg ggaaatgctg aaatcaagga acaaagtagg aaattcttta
    35281 atttatttta caactggaat tcatgggaaa aatatacgaa gaatatgaag gaaatacaaa
    35341 aagaatttcc tcagatagta aagtctagtc ctgttgatat ctctggcaca atggtaggta
    35401 ctggtaatag tccaatatct agctatactg ctgattacta tacaacaaat caaaaaggta
    35461 aaatggttga attgattaca cagacaagaa atactccaac aacagcaaca acaacaattt
    35521 ggcgtggagt aacaagcata ggagaaaatc aattgtttta cttggaggat ttagatagct
    35581 atgtaagagc agagtaaaat aaaaaaagag ctaacggtac caacgttagc tctttgtgct
    35641 gacgatgata acaaaaccta ccgaaaaagg attgtttcat cacaaacaca aacagaaaag
    35701 aattgataaa aaacccgaac ctcagaattt ttattaatca attcattttg attataataa
    35761 attgagctga acattacaat acgtatatgt tgtaaaacgt aaaaaaagtc aaaagtaaat
    35821 actttcttaa aaagtcaaat aaaactatta aagacaatat atatggttta tttgacataa
    35881 gaaaatggtc tagatgataa ttagaatata gaattgtaga cttaaataaa tcataaaaaa
    35941 ccttctatga taagttatag ctcttaattt taagcagttt tagatataag aaagtaaagt
    36001 gggggagaat aataatggca gatcgtaaat tagaaaagtt attagaagaa acgtgggatc
    36061 caaaagaatt tagtgaattt tttatggaga attttgaaac tgacttagcg gtgattgtta
    36121 aagatgctct tagagagcaa gggtatcctg aaactgcaaa ttatataaac attaatttta
    36181 ctttgtatac tgaaaataga ggaacttggg atttttgggc taacttagca aataaggagt
    36241 taagtgataa atcagataca ggaattcgta acttttttga atcaaacaga gatgattata
    36301 tgtacgctaa caatcaaaat aaattgaact ttagagtaga gtttgatgaa acacctgaag
    36361 aatttataga aagacaaccg cctaaagaaa atgtagcaaa ggtattggaa gatcgttgga
    36421 attcagatga aatagtttca acaattagcg aacaagacgg acaatatgaa ccgctagtag
    36481 aagcggttag agaagagcta agactgaata agtttcctga tgttcaaaat attgatgttt
    36541 ctcaaattga aataaacgtc aatatcacca atagacttga ttatgatagt tgggcagata
    36601 tagcattaga aaaatatatt tactcaacgt taaaagagtt tatcgaaaat agaatgaata
    36661 tcatgtatct acagcatcca cagtatttaa actttggtgt tgaaatagct acacctcttg
    36721 aagaatggaa aatggagcaa gggcttgact agcctttgct ccattttttt attttacaaa
    36781 tgaatacagt tcttcgtctt taccaagaat gattgtttgc ccaactttga gattatcagg
    36841 tgatccttca gcggtttgta aggagaacgt tccattgaac ttatcggata ccgttatagg
    36901 tagctttaat tctgatcctt cctcatcttt gagaattgct gttttccgtt catcaaaata
    36961 aacagtcttt attagataat ttttagtagt ggtttcatca gatttgtctt tgtttttagt
    37021 agtactaatt tctttagaat tagatgtatt ttctatgact atttcgtttt tggtatttgc
    37081 gcttgtattt atttgattga aaattttaat cgacaagatc ccactaagaa tagccaacat
    37141 agaaacaagt gtaattaata ctgataattt cctttttttg ttcatttctt cttaacttcc
    37201 ttttttgttt tgattagtta gatattcctt atctaataag cctttttagg ctattaaatg
    37261 aagataaacc ccaaaaaatt attgcaataa tgatagtgca atggtactat tattataaaa
    37321 taaattttgc gaaaggggta ggtctaaatc gcaaataaaa agaaattgaa cctagacaaa
    37381 tacaacttgt ataccgcaag ggaagcaagt ctaattttgg gtaaaggtag aaattatgtt
    37441 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
    37501 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
    37561 aaaaacaaaa agaaggaatg aatatgaaga aaaattcgct tgctatggtt ttatttgcat
    37621 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
    37681 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
    37741 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
    37801 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
    37861 gacgaacttt caacatcaat aaagatacat taacagttaa ggaagcagat ggcgaagtag
    37921 ttagtcaaac tgttcaaaaa gataaacctg atcctgttga aaatacggag caagagaaca
    37981 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
    38041 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
    38101 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
    38161 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
    38221 atcaatcaca aacaggttca agtgatgaaa ctgacagttc tacttcacca agtaaccccg
    38281 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
    38341 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
    38401 taatgacttt tgttaaatca atgccactat ctcctgataa taatccaaat aatggttttt
    38461 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
    38521 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
    38581 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
    38641 aacctgctca acataaatca atgttttggg gcgatgctat ttggaatcga catgtagtag
    38701 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
    38761 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
    38821 gttaataaaa gagatcgttt atatactaag tagttaagta tagtctttat ttgtccttgc
    38881 ttaactactt agaaaacttg ttgctcatgg ttggtagaac taaaaggggt gatgctatga
    38941 gtactagagc gttgaaacag cttgctttca acgaaaaaag tgattaatag gtgtggtgat
    39001 tttaacttag gaatgggcgc gaaggtcatt accacaccga catatcgtgg ctcattttct
    39061 tcgcctaact ggtataaaga aggagtaacg agagatgaag catgaaaaaa cgataatttt
    39121 aaaatcaaaa ataatttatt gttttgcgac tttagtaatg gtgttatcta cgcttataat
    39181 gcctttttca gcaataagag ctgaagcaga tgtcacaaaa ccgacaagtg caaaatttac
    39241 agaaactggc gtaaaaacag acggttatat tagggttaat atgccgaatc atccaaatga
    39301 atggatgatt tcaagtcaat ttaaagattc tcatggaaat attggatatt gtatggattc
    39361 agaattgcca tccccaacag gaagtggagc cggaagcttg aagtacaagg gagctggcag
    39421 tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca aaaacagcaa aggaactggg
    39481 tgcaggaaat gatacagaag catggtatgc aacacaatta gtttcatggg ttttagccgg
    39541 aaactttaaa gtttcacaga ttgtatggtc acatccaaac cataccgccg cagaaacagc
    39601 tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct aagaatggaa aagatacgcc
    39661 caacactgag ttttctatca cagcttctaa gacagctgac gaaggaaaat atcacacttt
    39721 cacatataaa acagcatcaa ataagactgg taatgcgaag ctcactttta catcagcaaa
    39781 accagctggt atgaagattt atgatgcaga tggtaaagaa attactaaca acactgtgaa
    39841 attaaattca agttttacta ttaaagtacc tgtgactaca ccaagtggta ctttgtcatt
    39901 taaaggtact gctaatgtta gcacaaccaa tccttttact tttgatggac gaggtgtgta
    39961 tcaagatgcc gtagttatga tcactacttc agaaacaaag gactctaaat ctttgagtgc
    40021 taaatggaca agagctgttg gtcaggtaac agttaagaaa gttgatcaag atggaaatgc
    40081 tttaaaagga gcggagttta ctctaactga caataacaat gctaaatctg ttaagacaac
    40141 cggagatgat gggttagcta cgtttgattt gcaacaaggc ttaacataca gcttagaaga
    40201 aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag gatggtatta ctgttaatac
    40261 cgatggacaa gaatttagtt acactgctac gaacacaatt aaaaaaggta aagtaaaagt
    40321 taaaaaggtt gaccaagatg gaaaaccatt agctaatgct gaatttacct tgacggatga
    40381 ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga cttgcaagct tcgatattgt
    40441 agcaaacaag acctacagcc ttgaagagac cggagtacca actggttata caggcgtatt
    40501 caagcaagaa ggaattaccg ttaaagatga tggacaaact tttgaatata cagctacaaa
    40561 cactttgaaa aaaggtttag taaaagttca taaagtcgat caagatggaa aacctttggc
    40621 aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca caagtagaaa aaacagatga
    40681 aaatggactt gcaagcttca atattgtagc aaacaagacg tatagcctag aagaaactgg
    40741 agtaccagta ggttacacag gtgcatttaa gcaagagggt atcaccgtta aagacgatgg
    40801 gcaaactttt gaatatacag ctacaaataa tttgaaaaaa ggtgtagtaa aagttcataa
    40861 agtcgatcaa gatggaaaac ctttagcaga tgcagaattc accttaacgg atgaaaaagg
    40921 tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct acgttcaaca ttattgcgaa
    40981 caaaacttac aacttagaag aaacaaaagt accaacaggt tacacaggcg cattcaaaca
    41041 agaaggaatt accgtcaaag atgatggaca agtctttgaa tatactgcta aaaacacttt
    41101 gaaaaaaggt gtgatcaaag ttcataaagt ggatgatcaa gggaaagcat tagcaaacgc
    41161 agaattcact ttgacagacg acaaaggtgt aaaagttgta caaaaaacgg acagcaatgg
    41221 cttggcaaca tttaatattg ttgccaacaa aacgtacaaa atcgaagaaa cacaaaaccc
    41281 taatggttat acagggaaat ggtctaaaga aggcatcacc gttgctgacg atgaacaaac
    41341 ctttgaatat acagtaatta atcaccttgt acctgaaaca gtaagaacag gcggaatcgg
    41401 tacatcagcc ttccttcttg tatttctaat tgcaggattg agtatggtgc tactgtatta
    41461 tcgagaacag cacaaagcaa aataaaaaag tgttaatgga aaagaagtaa atatgaaaaa
    41521 agaaggctga cgtatgaaat caaaaaaaat tttttcaaat ttaatgctat ttggtacttt
    41581 gctaggagtg ggattgggcg caagctcaat ctcttctagc cttggactag gggggcaagt
    41641 agcatatgca caacaaaact ataattatca aggaactgga acgattcata tccataaagt
    41701 aacaaatact aatggggatg ggaaccctgt ctcagatatt gataacaaag ggaaagaaat
    41761 agctttacca aatggtgtaa aacctcttga aggcgttgag ttctcttatg tgaaacttac
    41821 taaagatgaa gctaaatcct tgggatggac ggatagtgat actacaaaag gtcaacatat
    41881 tgatgatgct aaagcacgat tattactaaa caaaaaatac agtgaagtaa gtagaatgaa
    41941 gactgattca caagggaata tagacatacc tatacaagta aatacagacg atttaacaga
    42001 taacttgtat ttgattgttg aaacaggaac agtcaaaggt gtagttgaag aagcgtatcc
    42061 tatgattgtt aacgtacctg aactaaacgg atcagatgat caatacttta attatgatgt
    42121 acatgtctat cctaaaaact atttccaatt aaactcagca gagcttgata aaaaagacga
    42181 agatggaaag gctcttgccg gaactgtttt tgaattgcga aaagatgatg gatctgatca
    42241 ggttgttgga acttataaaa cagacagcac aggtaaatta actgtagatg gtttggaatt
    42301 aggtgactat tatttcctag aaaccgactt gggaacagat gctaataaaa caacctattt
    42361 actagatggt aaaaaattca aatttagcgt taaacgtggg gagactgcta aagtagttct
    42421 tgatgttacc aactacaaac gacctgtaat tactaaggta attgataaag aaaccgttga
    42481 tactggcgaa gatgttcaat atacattgaa accatcaatt cctggtaata ttggtaatta
    42541 cgaaaaatat gttgtcaaag atgttctagg tgcagagtta acttatgatt catcagaagt
    42601 gatcgaagga atgactttag atactgatta cagtatcgaa cgtgacggac aaacgacaaa
    42661 ctatgtcttt actgaatcag gtatggataa gcttcagaaa caatttgatg caggggatcg
    42721 cttctttaca attttgatga acgctcatac taatagtaaa gctaaaatgg atacaccact
    42781 taagaatgat gtttcaacga tctacaacaa tggatatgaa cccgaagacg aaacaccagt
    42841 tgatcacgct tatacaatca ttggtgatca ccaattcatt aaaacagatg gagatacaag
    42901 caaagcattg agtggagcaa aatttgtagt gaagaacaaa attgatccga ataaagttac
    42961 ttacctaaaa caagatccaa caacgaaagc tgttacttgg attgcagata aatctaaagc
    43021 tactgaattc gtttccggat cagacggtaa gttccaaatt gaaggcttgc aatataccta
    43081 tgatgtagat tttgataaac aaggtaatcc aacaatcaca gataaaaaaa tcaatgatta
    43141 tgctcttgaa gaagttgttg ctccttcagg atatacacca actagaaatg atgttccatt
    43201 tgtggtatcg aatgatgatg cttctagcga agtaacagac atcaagaacc ttaaaacacc
    43261 tgaaacagtt cgtacaggtg gaattggaac agtagtattt ctagtggttg caggtgtggc
    43321 aggaacaggg ctatatgttg tctacaaacg agaacaacac aaagcttaat gaaataaaat
    43381 tccctagagt gtttttggct ctagggtttc tttttaaaaa gtgtgtgaaa agtaggtatg
    43441 agcatgaaaa agaagaatat caagttgatc atcttaaaaa ttgggatgtt gctatgttta
    43501 ggagtaggtc tatatcctgt aattactcag gcttatacct actaccaaac tcaacaaaca
    43561 ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa aggagaatga agaagtcttc
    43621 aagagatatt cagaagttaa taggaaacga aaagacacag aacaaacctc agtcacgatt
    43681 cctgaagata ataacaatat tccgtcaaat gtaaatcttc agaaggataa attaggtgaa
    43741 ttagtgggtg aaattcttat accaaagatt aatgtggatt tgccaattta tgatggtgta
    43801 aacgattttc aactatcagc aggagcagga atattaaccg gaactgatta tccaactggt
    43861 gggattggaa accattctgt tatatcagct cattcgggca ttcctaataa aaaatatttt
    43921 acagatttag agttgctcaa acttggtgat aaatttttta ttgatatttc agggaaaaag
    43981 cttgcttatc aagtagatcg gataaaaaca atcctaccaa ccgaatttga ggatttgctt
    44041 cctgtggcag gaaaagatta tgtaacactt atgacatgta cacctgttgg agataattct
    44101 cataggttac tcgttagagg ggttagcata ccttaccaaa aaagtattgt tgagaaagaa
    44161 aagaagaaag aagatttaaa ggtaaaggcg cagatgaact ggtatcctat cgtagttgta
    44221 ggggttttat taataatagc tgtgatagtc attattaaaa aaatagtagc aaagagacga
    44281 ggaatgaaaa atgaagaata aaatactact ttttttgact cttatttgca ctttggtttt
    44341 aacaatttat cctgcaaatc agctctatca tcaattcaaa acaaaagaga atgtggcgga
    44401 aatccaagag aaaattgaga aaaaacaaac tccttcaaaa gaggaagtgg caaaaaaagt
    44461 cgaacaaata aaatcgaaca gcggtaacca aggattagac aatactcaga aaacagacgg
    44521 cggatcattt gatattggtg aagctgaagg aaaggtcaat ccttacacag gagatgtttt
    44581 ttcaaaaaat caaacgaaag attttgaacc atctacctat tcaggggcgg ttggttatgt
    44641 ctatattcca aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggaacattt
    44701 atatagaggt gtggcacaga tgagtgctac tagtctacct ttatcaggta aaggtcatca
    44761 aacagtaata gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca
    44821 attagtagcc ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt
    44881 tttccgaaca gacgttatta gaaattacgg tcagaattca acactgactg atattcctga
    44941 tttagatatg ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt
    45001 agttagaagt ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa
    45061 taccgcatac gctgatgcca ttgatagcga gtccgtttca gataagacag aaactgttac
    45121 atcttctaat gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa
    45181 tgatcttaat attccggaac aaggtagcgt agagatgcaa gacagaacat ttgctgaaaa
    45241 ggcaagttca atattcacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg
    45301 gactgtaata ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttcg
    45361 aaaagcataa tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt
    45421 attgatctct tttttatttt gaatagagct agattcactt ttaaataaaa tcaaaactga
    45481 ttttaattct aaagaaaaag tcaagaaaaa aatacaacaa atttttttat cagaatattt
    45541 atcagaatat t
//
LOCUS       DANKAY010000012        41012 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000012 DANKAY010000000
VERSION     DANKAY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWY98_000666"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWY98_000666"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2036440.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWY98_000667"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWY98_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2036441.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWY98_000668"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWY98_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2036442.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWY98_000669"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWY98_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2036443.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWY98_000670"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWY98_000670"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2036444.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWY98_000671"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWY98_000671"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2036445.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="PWY98_000672"
     CDS             complement(8557..9027)
                     /locus_tag="PWY98_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2036446.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="PWY98_000673"
     CDS             complement(9073..9498)
                     /locus_tag="PWY98_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2036447.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="PWY98_000674"
     CDS             complement(9565..9834)
                     /locus_tag="PWY98_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2036448.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="PWY98_000675"
     CDS             complement(9845..11440)
                     /locus_tag="PWY98_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2036449.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWY98_000676"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWY98_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2036450.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="PWY98_000677"
     CDS             complement(14993..15553)
                     /locus_tag="PWY98_000677"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2036451.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="PWY98_000678"
     CDS             15906..16880
                     /locus_tag="PWY98_000678"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2036452.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="PWY98_000679"
     CDS             17050..18450
                     /locus_tag="PWY98_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2036453.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="PWY98_000680"
     CDS             complement(18528..19250)
                     /locus_tag="PWY98_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2036454.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="PWY98_000681"
     CDS             complement(19329..19835)
                     /locus_tag="PWY98_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2036455.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="PWY98_000682"
     CDS             19958..21757
                     /locus_tag="PWY98_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2036456.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23435)
                     /locus_tag="PWY98_000683"
     CDS             complement(21825..23435)
                     /locus_tag="PWY98_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2036457.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23690..24199)
                     /gene="mreD"
                     /locus_tag="PWY98_000684"
     CDS             complement(23690..24199)
                     /gene="mreD"
                     /locus_tag="PWY98_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2036458.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24203..25057)
                     /gene="mreC"
                     /locus_tag="PWY98_000685"
     CDS             complement(24203..25057)
                     /gene="mreC"
                     /locus_tag="PWY98_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2036459.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25212..25850)
                     /locus_tag="PWY98_000686"
     CDS             complement(25212..25850)
                     /locus_tag="PWY98_000686"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2036460.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25851..26501)
                     /locus_tag="PWY98_000687"
     CDS             complement(25851..26501)
                     /locus_tag="PWY98_000687"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2036461.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26514..27413)
                     /gene="rsgA"
                     /locus_tag="PWY98_000688"
     CDS             complement(26514..27413)
                     /gene="rsgA"
                     /locus_tag="PWY98_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2036462.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27578..29647)
                     /gene="pknB"
                     /locus_tag="PWY98_000689"
     CDS             complement(27578..29647)
                     /gene="pknB"
                     /locus_tag="PWY98_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2036463.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29644..30384)
                     /locus_tag="PWY98_000690"
     CDS             complement(29644..30384)
                     /locus_tag="PWY98_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2036464.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30397..31755)
                     /gene="rsmB"
                     /locus_tag="PWY98_000691"
     CDS             complement(30397..31755)
                     /gene="rsmB"
                     /locus_tag="PWY98_000691"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2036465.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31755..32693)
                     /locus_tag="PWY98_000692"
     CDS             complement(31755..32693)
                     /locus_tag="PWY98_000692"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2036466.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32686..33177)
                     /gene="def"
                     /locus_tag="PWY98_000693"
     CDS             complement(32686..33177)
                     /gene="def"
                     /locus_tag="PWY98_000693"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2036467.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33192..35600)
                     /gene="priA"
                     /locus_tag="PWY98_000694"
     CDS             complement(33192..35600)
                     /gene="priA"
                     /locus_tag="PWY98_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2036468.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35853..36158)
                     /locus_tag="PWY98_000695"
     CDS             complement(35853..36158)
                     /locus_tag="PWY98_000695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2036469.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36163..36777)
                     /gene="gmk"
                     /locus_tag="PWY98_000696"
     CDS             complement(36163..36777)
                     /gene="gmk"
                     /locus_tag="PWY98_000696"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2036470.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37264..37548)
                     /locus_tag="PWY98_000697"
     CDS             complement(37264..37548)
                     /locus_tag="PWY98_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036471.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37566..38456)
                     /locus_tag="PWY98_000698"
     CDS             complement(37566..38456)
                     /locus_tag="PWY98_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2036472.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38476..38694)
                     /locus_tag="PWY98_000699"
     CDS             complement(38476..38694)
                     /locus_tag="PWY98_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2036473.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38664..40364)
                     /locus_tag="PWY98_000700"
     CDS             complement(38664..40364)
                     /locus_tag="PWY98_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2036474.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40631..>41012
                     /locus_tag="PWY98_000701"
     CDS             40631..>41012
                     /locus_tag="PWY98_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036475.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYF"
BASE COUNT        11117 a         8873 c         7427 g        13595 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    22501 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22561 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22621 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22681 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22741 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22801 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22861 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22921 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22981 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    23041 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    23101 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    23161 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    23221 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    23281 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    23341 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    23401 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    23461 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    23521 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23581 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23641 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23701 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23761 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23821 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23881 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23941 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    24001 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    24061 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    24121 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    24181 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    24241 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    24301 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    24361 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    24421 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    24481 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24541 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24601 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24661 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24721 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24781 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24841 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24901 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24961 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    25021 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    25081 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    25141 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    25201 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    25261 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    25321 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    25381 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    25441 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    25501 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25561 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25621 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25681 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25741 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25801 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25861 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25921 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25981 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    26041 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    26101 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    26161 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    26221 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    26281 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    26341 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    26401 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    26461 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    26521 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26581 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26641 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26701 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26761 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26821 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26881 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26941 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    27001 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    27061 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    27121 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    27181 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    27241 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    27301 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    27361 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    27421 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    27481 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27541 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27601 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27661 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27721 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27781 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27841 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27901 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27961 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    28021 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    28081 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    28141 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    28201 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    28261 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    28321 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    28381 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    28441 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    28501 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28561 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28621 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28681 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28741 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28801 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28861 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28921 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28981 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    29041 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    29101 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    29161 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    29221 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    29281 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    29341 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    29401 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    29461 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    29521 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29581 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29641 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29701 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29761 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29821 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29881 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29941 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    30001 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    30061 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    30121 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    30181 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    30241 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    30301 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    30361 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    30421 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    30481 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30541 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30601 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30661 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30721 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30781 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30841 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30901 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30961 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    31021 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    31081 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    31141 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    31201 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    31261 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    31321 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    31381 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    31441 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    31501 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31561 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31621 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31681 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31741 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31801 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31861 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31921 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31981 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    32041 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    32101 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    32161 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    32221 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    32281 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    32341 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    32401 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    32461 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    32521 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32581 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32641 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32701 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32761 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32821 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32881 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32941 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    33001 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    33061 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    33121 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    33181 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    33241 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    33301 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    33361 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    33421 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    33481 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33541 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33601 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33661 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33721 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33781 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33841 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33901 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33961 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    34021 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    34081 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    34141 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    34201 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    34261 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    34321 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    34381 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    34441 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    34501 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34561 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34621 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34681 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34741 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34801 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34861 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34921 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34981 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    35041 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    35101 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    35161 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    35221 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    35281 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    35341 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    35401 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    35461 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    35521 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35581 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35641 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35701 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35761 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35821 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35881 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35941 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    36001 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    36061 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    36121 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    36181 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    36241 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    36301 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    36361 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    36421 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    36481 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36541 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36601 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36661 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36721 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36781 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36841 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36901 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36961 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    37021 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    37081 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    37141 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    37201 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    37261 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    37321 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    37381 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    37441 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    37501 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37561 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37621 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37681 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37741 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37801 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37861 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37921 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37981 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    38041 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    38101 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    38161 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    38221 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    38281 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    38341 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    38401 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    38461 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    38521 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38581 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38641 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38701 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38761 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38821 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38881 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38941 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    39001 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    39061 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    39121 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    39181 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    39241 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    39301 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    39361 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    39421 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    39481 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39541 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39601 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39661 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39721 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39781 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39841 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39901 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39961 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    40021 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    40081 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    40141 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    40201 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    40261 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    40321 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    40381 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    40441 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    40501 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40561 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40621 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40681 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40741 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40801 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40861 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40921 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40981 ttataaacta cagaataatt ttgtatactt cg
//
LOCUS       DANKAY010000013        37307 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000013 DANKAY010000000
VERSION     DANKAY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            55..732
                     /locus_tag="PWY98_000702"
     CDS             55..732
                     /locus_tag="PWY98_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036476.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            735..1862
                     /locus_tag="PWY98_000703"
     CDS             735..1862
                     /locus_tag="PWY98_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036477.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2062..2502)
                     /locus_tag="PWY98_000704"
     CDS             complement(2062..2502)
                     /locus_tag="PWY98_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2036478.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2513..4546)
                     /locus_tag="PWY98_000705"
     CDS             complement(2513..4546)
                     /locus_tag="PWY98_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2036479.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4548..6251)
                     /locus_tag="PWY98_000706"
     CDS             complement(4548..6251)
                     /locus_tag="PWY98_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2036480.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6345..7295)
                     /gene="manA"
                     /locus_tag="PWY98_000707"
     CDS             complement(6345..7295)
                     /gene="manA"
                     /locus_tag="PWY98_000707"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2036481.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7334..8359)
                     /locus_tag="PWY98_000708"
     CDS             complement(7334..8359)
                     /locus_tag="PWY98_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2036482.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(8568..9437)
                     /locus_tag="PWY98_000709"
     CDS             complement(8568..9437)
                     /locus_tag="PWY98_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2036483.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9896..11788)
                     /locus_tag="PWY98_000710"
     CDS             complement(9896..11788)
                     /locus_tag="PWY98_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036484.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11785..13512)
                     /locus_tag="PWY98_000711"
     CDS             complement(11785..13512)
                     /locus_tag="PWY98_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036485.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13528..14178)
                     /locus_tag="PWY98_000712"
     CDS             complement(13528..14178)
                     /locus_tag="PWY98_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2036486.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14382..14678
                     /locus_tag="PWY98_000713"
     CDS             14382..14678
                     /locus_tag="PWY98_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2036487.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14850..15470
                     /locus_tag="PWY98_000714"
     CDS             14850..15470
                     /locus_tag="PWY98_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2036488.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15562..16728)
                     /locus_tag="PWY98_000715"
     CDS             complement(15562..16728)
                     /locus_tag="PWY98_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2036489.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16740..17093)
                     /locus_tag="PWY98_000716"
     CDS             complement(16740..17093)
                     /locus_tag="PWY98_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2036490.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17221..18243
                     /locus_tag="PWY98_000717"
     CDS             17221..18243
                     /locus_tag="PWY98_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2036491.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            18262..18423
                     /locus_tag="PWY98_000718"
     CDS             18262..18423
                     /locus_tag="PWY98_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036492.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(18682..19116)
                     /locus_tag="PWY98_000719"
     CDS             complement(18682..19116)
                     /locus_tag="PWY98_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036493.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19226..21085)
                     /locus_tag="PWY98_000720"
     CDS             complement(19226..21085)
                     /locus_tag="PWY98_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2036494.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21203..22363)
                     /locus_tag="PWY98_000721"
     CDS             complement(21203..22363)
                     /locus_tag="PWY98_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2036495.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22670..24094)
                     /gene="araA"
                     /locus_tag="PWY98_000722"
     CDS             complement(22670..24094)
                     /gene="araA"
                     /locus_tag="PWY98_000722"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2036496.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24110..24808)
                     /locus_tag="PWY98_000723"
     CDS             complement(24110..24808)
                     /locus_tag="PWY98_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2036497.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24765..26426)
                     /locus_tag="PWY98_000724"
     CDS             complement(24765..26426)
                     /locus_tag="PWY98_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2036498.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26644..28044
                     /locus_tag="PWY98_000725"
     CDS             26644..28044
                     /locus_tag="PWY98_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2036499.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28296..29375)
                     /locus_tag="PWY98_000726"
     CDS             complement(28296..29375)
                     /locus_tag="PWY98_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2036500.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29461..30420)
                     /locus_tag="PWY98_000727"
     CDS             complement(29461..30420)
                     /locus_tag="PWY98_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2036501.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30432..31943)
                     /locus_tag="PWY98_000728"
     CDS             complement(30432..31943)
                     /locus_tag="PWY98_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2036502.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(31965..32528)
                     /locus_tag="PWY98_000729"
     CDS             complement(31965..32528)
                     /locus_tag="PWY98_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2036503.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32534..33397)
                     /locus_tag="PWY98_000730"
     CDS             complement(32534..33397)
                     /locus_tag="PWY98_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2036504.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33411..34307)
                     /locus_tag="PWY98_000731"
     CDS             complement(33411..34307)
                     /locus_tag="PWY98_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2036505.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34482..35771)
                     /locus_tag="PWY98_000732"
     CDS             complement(34482..35771)
                     /locus_tag="PWY98_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2036506.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35743..37260)
                     /locus_tag="PWY98_000733"
     CDS             complement(35743..37260)
                     /locus_tag="PWY98_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2036507.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        10806 a         7805 c         6453 g        12243 t
ORIGIN      
        1 tttagatgtt ttccattcta ttatgttatt aaattttata ttggaggcct atctatgaca
       61 ttactgatca aaaacatgaa catgaaacgt ggtaaaaaac gtgtgattcg acaattgaat
      121 cttttagtag aatcaggaga agtagctgct ttgattgccc cgaacggatt tggcaaaaca
      181 actttgctga atggcattgc ccaactgctg ccaacaactt atgatcaatt gtcccttcac
      241 aacatctctc ctactgctta tacagattat cgccggtctt tcttttttct agaatcaacg
      301 aatcagttat ccgactatct atcggctgtt gattatttga ctttgatcaa atactattgg
      361 cagagcaaag tagaatgcca aactgttttc caacgattga agatgactga gtaccttact
      421 ttgccgattg gaaaaatgtc tctaggaatg aaacaacatg tcttgcttgc tgcttacttg
      481 atcagtgatt catctgtcat gttattcgat gaaccgttga acggattaga cccaggaagt
      541 attgatatct tgaatagcct catcagacaa tggcagaaag aggggaagac gattcttctt
      601 tcttcccacg ctatcgcgaa tatccaatcg atttgttcta aagcatgttt tctaaaaaat
      661 ggagaaatcg tcacagaatc ctcaaatatg gaagagattt tagctattta tcgtaaatta
      721 tatgaggtgt aactatgtct ttacttacat ttgaacgcaa tctcttacta aaaaacaaag
      781 taaacttttt atttcctcta ttgttggtcg tgctctttgc attcccactt ttttttgatc
      841 ataaacttgc ttatacagaa ttcgatgaac tgaatcataa ctatgaagag atgcaacgtt
      901 tgattgaaac gttgaaagaa gatgagaatg agaaagaatt tgtggaaagt ttagagaaaa
      961 gcaacaaact gatcgaagca atcttacatg caaaaaacac agggaatgtc cagcaaatgg
     1021 tcgaagcaac ttatcattat gaaaaagata tactggatcg actgatttct ggtcaaagac
     1081 aaggaattcc aatcattgaa caacaaaaac gtgtcgagtt gctccgttat atgaaagaac
     1141 atcagatcca aagatattct atttttgact tgccagcgca tctcccttta gcaaattact
     1201 acgaaaatat tttttcagga atgatttcta gttttttgat tttatgtata accgccttgt
     1261 ttctttcttc catcatctct tatgaaaaaa gaaagcaggt aatctctcta gtcaatctat
     1321 taccggatag tatggtaaag aaacatagca tccgttttac catttattat ggtgcagcta
     1381 tgctaagtct cgtgatgcca tttttgatcg tctctatact agtaataatc aaaaatggtt
     1441 tgggagactt ccgttatcct gtaggaacga tcattggtca ggagatacga atccttccga
     1501 tgtatgagta tcttttccag agctttctgt tcctgttgtt gtgggtgctc tttttaagta
     1561 caatcagttt tcttctatct gctttatttg aacattctct agtcaatctt ttaggtacac
     1621 ttctttgtct tttcttagca gagtatcgct tgttttcttc gattggatgg atagaatcaa
     1681 tcagtcatta cttgccaact tcttatgttg attttcaaaa tgtgatcata ggaggagata
     1741 ttttctcgcc actagcttca gaacaggtta ctttcatgaa tggtatcttg acacttggta
     1801 tatggagtat cgttttatta ttcataggta tgggcactat ctatataaaa aagagctatt
     1861 aaaacacaaa aataataggg gcggtgacaa aaaccttgtc accgtcccct atttcaaata
     1921 aacggttttc tagaataaat agttttcaaa agcttgcatc cgcagtaata agaacctccc
     1981 aacaaaaata gctcctctta tttttatctt ggttgaagtt attacttgat gcaaggcagc
     2041 tttttttact gtgaacattg gttatatatg tccatgttca aaaatcattt ggacttcttc
     2101 aatggttttc gcatcactga gcgccattac ttggtcgatg tctgaacaga aaaaggctaa
     2161 atcttggagc accgctaact gatgttcttt ttttaaagcc actacaaata agagtgtcgc
     2221 gatttttggt tcctcttttg ttccaaagtc cacaccatca ggcacttgga ctaaatagat
     2281 tccttcttct actacttctt catctttgtc agtcccatgt ggcaaggcta cgaaattccc
     2341 gatatagaca ctcgcttttt cttgtctttc gatcattgat tggatatatg ccggtttgat
     2401 tcccccttgt tgggtcatcc actggccgat ctcggtcaac gcttctttcc aattttgata
     2461 ggattgatcg agcaatattt tttctgctgg tagctgtatc atcttttcac cttcatttct
     2521 ttatttcttc aatcaggatt ttcgctaaca actgataaac gatttcttta tttcctgagc
     2581 ggaagatctc ggtgttcaag tcattcatga tgattgctcc actgatcttt cctaaaagaa
     2641 tcccgtcttt ttcttggatt ggcgaaggcg ccagcataac taacatacgg gacaaatcga
     2701 ctggctgtct atccatccct tcaatggtca acggatgttt caaattgaaa atacaaaaga
     2761 tcggtttaga aatcaatgag ctggatgtat ggaataaccc catatttgta tgaggaatac
     2821 cgataggtgc ttgttcatat cgcttaagca gccggcgatg aaccacctct tcatccgtta
     2881 ctagtccttc aggaaaataa ggaaataagc tgcgaatcgt ttcggaaatc gtctcaggat
     2941 tatccagttc ttggataaaa aaacgaccga gtaattgatt gatttgatcc atgaaatgca
     3001 ttgtttcctc ataagtatct tctatcatcc actcgttatt gctgttatac gtaatttttt
     3061 gccttttttt cttctcaata tgcgaaaaag cttcttttag ctgcttgact tcatcttcta
     3121 gcagtagcgg ggagactaaa atatattttc caccgtatcc tggtaaaata gatgtagaaa
     3181 taacaacatc atattcttct tgtaaatcga ttttttttaa atcagcaatc cgatagaaat
     3241 gaatttgttg gatgaaggga aaataccttt tcaatcgtag ttccaacatg ctagtagaag
     3301 ctaatccact tggcgaaaat cctgcaatac tgatattgct ttctttcgga cttcgttcca
     3361 atgaattcgc aaaatgaagg accatataag cgatctcttc ttcggataat tttttttctt
     3421 gaaaaatccg ctctactgct ttacggattg cattcgcaac ttcctcgtat tgaaccatga
     3481 ttttttctaa aatcggattg gtcagtgtct cttcttgtaa aacggctctt gaaaacacac
     3541 cagataaatg agtcaacagc attttgtaca aagtttcatc ttcataaaaa tggacattcg
     3601 tctcttcact tacttcttga atcagttgtt ttactaaata agcaagctgt gtatcgaacg
     3661 tctcttcaaa gaaattctga tcaaatgagt tcgtgaagtc atttaataat cctgcaaaaa
     3721 agacgatctc atttactgga taaagttgtt ttgtctcaga agcgatccgt gaaaacaatc
     3781 gtttagctgt ctgtagtaat tctttgtttg ccagactatt atatgtctca tttgtcagta
     3841 aatgattttt agtcacacgc tccatcgtca atgtcagcat caaaaccaaa tgttcaagct
     3901 ttctatcact tagattggtc tttttttgga tcaattcttc cccgattact ttgcgtgcaa
     3961 gagatagatg ttgcggatca acgaatcgaa aaaaataatt cagtgatgca tcgaaagaaa
     4021 tactatgaaa aagttcatat tcattgattt ctaattctaa tacattcgct gtcagaagcc
     4081 ggcggtattt ttccggtcct tcgatctcgt atccctggtt ccgaatgatt tccaaaggca
     4141 gttggcgaat actttcttcc aactgtttga tatctgcata aaaagtggta ttactgatgc
     4201 gatatgattc catgaaatga gcaagactca acggctctgt cgtcagtaac aactcggcta
     4261 aaatcgcgtg ttgtcgttta gcagtggata agtcattact tttcccatct gtattttctt
     4321 ttaattgctc taatacttct tctgatgcct caatgacaaa tgagcctcta gctgcttttt
     4381 tcaaagaagc atttaccgat ttcaatgaga cttcaagatt atctaattcg cggtaaatag
     4441 tccgtttact gaccttcaat aaattcatca ttttttggat agatacaggt gacgggctac
     4501 tgagaaattc tgctaataat tgtttttctc tgggcgataa atacatctta tatttctccc
     4561 ttcacgagtt tttcgtattc attcgtagct acccaatgtt caactagaac tagccgaggg
     4621 atcttttctt ctagctgttc agttacagct agttctcttt gtgcgatata aatcgtttct
     4681 tcttggattt tgattttttc tattgattct tttgagataa caaactgttt tttcgctttt
     4741 tttgctaatt gacttaaaat cgtcatgccc atagtttgtg agccgcgtct gttgttttta
     4801 tacaaaaata caatattagc aggcgacttt ttttgttctg tgatatctgc tatctctttt
     4861 ttttcttccc gctttggaaa ttggatcgct tcttttattc gtgacagcaa gatatctgtt
     4921 tccaaaaagt gatccagtac atagatttga ctttgaggta ctttttctct tgcaagaccc
     4981 gctagttcat gttgcacgat aaccagctga tttacttggt tattcaattc atagatagag
     5041 ctgtatccga cagaaagatc gattttttct gctgctaatt tctttttcag cagggcggct
     5101 cccatagcac ttgatcccat acctgagtcg caagcaaaaa tgacggaatg aatgatagat
     5161 ggttccaaga tttctcccct ttctttgacc gttttgttct ctttttcttt atttggtaat
     5221 ttcaatagta tcgctgcaca aatgaaggtc acaaatgtac tgattgctat ccctatactt
     5281 accccagcta gttgtggata aggcgcattg gcaaaaatca cgagtagtga cccaggcgat
     5341 acaggtgcag ctaatcccgt atctaaccat tgaaatatca acgtgccgct tgccccacca
     5401 gccattacag ctaaaaatag atacggtcgc agcaagacaa atggaaaata gatttcgtga
     5461 atcccgccaa tcgcatggat catcaatgct ccactcgcat ttactttttc ctgtttttct
     5521 gcaaaacaaa ggatagaaag taagatccct aaaccaggtc caggatttgc ctccatcaaa
     5581 aataaggaag agtacccgac tgtttgtgag aattcgattc ctaaaggtgt caatacacca
     5641 tgattgattg cattattgaa aaatagtact tttagaggtt ctaagatcag actgacaagt
     5701 ggtaacagat tcattgtgac tagccaagac acaacggagg cgagcaacaa gtgaacacta
     5761 tttaaaactg gttcaataaa aacgattcca attatacaaa taacgctacc agtcagtcct
     5821 actagtaggt tgctgacaag catttcatat ccagccttga atttcacgtg gaatctagaa
     5881 acaaattgtt catatacgat gccggcaagc ggcccgataa tcatcgctcc tacgatttgt
     5941 ggagcattgg aacgagcgat caaccctaaa gaagcaattg caccggcgac tgcccctttt
     6001 tgtcccccta ccgatttccc gcctgaataa ctgatcaaga tgggtaaaag cagctggatc
     6061 atccaatggc atacctcttc taatgtggtg tgcaaatcgc cagacacacc ttttgttgct
     6121 aatgacaaga ttccccaagc gataaacaga cttagatttg gcatgatcat ttcatttaag
     6181 tgatgtccag cttttctcag ttgtttcata gacacgcgac tgctgttgtt tgctttccat
     6241 ttgtattcca tctttttcac catacttcta tttattgatg gacggatttt atgtagaaaa
     6301 agcagcaaac gaaaaaccgt ctgctgcttt cagctatctg ggaactattt gcctggttca
     6361 gtcgcaatga ttgctagttc accttggacc gcccactcag aaacgccatt tggaataata
     6421 cagcttgttc ctttttctaa tggatatgtt tttccgtcaa cgacaaggtc tcctgctcct
     6481 tcgattaccg tcatcaatgt ataaggggct tgttttttga aagatgtgat tcctttaata
     6541 tcccactcat atacattgaa aaactcagtt tctaaataag tgacgatcga tgaattccct
     6601 tttcggactt ctttgatttg taattctggt gttttcgcag ggacagttgt tacatcgatc
     6661 gattgttgga tatgcagctc tcttgtttgt ccttgcgcat cttttctgtc gtagtcatac
     6721 acgcggtaag tcgtgtcaga actttgctgt gtttctaaaa tcatgattcc tttaccaata
     6781 gcatggatcg ttccgcttgg tacataaaag aaatcacctt ttttaactgg tactttcttc
     6841 aaaaggtcat cccagcgccc atctttgatc atctcagcta gttcttctct tgtctttgca
     6901 tgatgaccat agatgatttc tgcaccaggt tcagcatcga tgatatacca gcattcagtt
     6961 tttcctaatt caccttcatg tttcatccca taggcatcat ctggatgtac ttgtacagat
     7021 agatcatctt ctgcgtctaa gatcttgatc aataatggaa acactggttc tgaaggatgg
     7081 ccaaacaatt ccttgtgttc tgaccatagt tcatctaatc ttttcccttt gaattcgcca
     7141 ttttctacta cactgacacc atgaggatgc gcactgatcg cccaatcttc tccgatttta
     7201 tcgttaggga tatcaaaacc aaagactgaa cgtaaacgac ttcctcccca aattttttct
     7261 tgaaaaactg gttttaaaaa cataggttcc tgcataattc aaactcctct catttttcat
     7321 agctattata gcattatttc ggtaataccg ttttctcttt caaaaagttt tcaacaattt
     7381 catctcgaca tatgagatac tccgcacgat gatcgacagg atgtacgcga tttgacaaaa
     7441 atatcaacgc ttcttgtgtt tttatatcaa tcaagaaaaa cgtacctgta taaccagtat
     7501 gaaaaagaat cgcatgaccg tcttctacat ccttttttaa gtcccatcct aaagaacgat
     7561 agccattttt tttcggtgtt tgatcttgca gtaaagaatc aatcgtctct tcttctaaaa
     7621 aacgaccatt acggattttt cccttgttca aatacatttg gacaaaagta atgctgtctt
     7681 tcaaatcaat aaagagaccc gcgttacctg catgttcagc caaaactctt gctttcggat
     7741 catgagtttg tccacgcaaa acctcacccg aaggcagttg ttctgtggga acgatttttt
     7801 tggaaggaaa aggaagaaat aagctgttca tcattcctaa tggttccaat acttgttctt
     7861 gaaagatttc cactgcagtt tcttggtaaa tttcctctag catcaaccca agaagaatcg
     7921 taccggcatc tgtatatttt acttcttttc ctagttgctc acctggttct agcttcaaat
     7981 aagcggcgat caattcttcc tgagatagct tatctctgtt tggtatccat gttttgatat
     8041 cagaagtatg tgtcagtaaa tgacgcaaag tgattgtctg attttcaaat tcaggaagat
     8101 atttatgaag cggttgatcg agttctatct ttccatcttc taatagacgt aaaactaatg
     8161 aagtggttgc aacgactttc gtcagtgagg ctgcatcaaa caacagatct tctgtcatca
     8221 gctgcttttc aggaacgact tgggcaaagc cggctgtaaa tgtttctact tgattttctt
     8281 ttatgaaggc gactgctaca cctggataaa atccttcttc catctttttc aaaataagct
     8341 gtttcgtgtg tggatacatc tttttctcct gcttttctat cagattctta cagtccaagc
     8401 ttaaagctta atctatgagg aatcaacctt ttttacacaa aaaaactgcg cgaaaaaccg
     8461 cgcagtttcc cccggtatac tattttctct ttctatctct agaataagcg atagtatgac
     8521 cgttcataaa atgacgagac atgcgacaat cgttaagaaa gaaaaagtta agcgtcgcat
     8581 cgaatgtcag ttttattctt ttcttttggc tcactgacga accaccattc gattcctata
     8641 gcatcataga ttgtgagtac tgtgtgcttt ttgtctaaga agaaatcaac tgatagttgc
     8701 tgcaagtgag caatcagtcc ttccatgctt tcagtatcga tcatgaattt cagaaaatca
     8761 agtccaatca cttcatctgt aggcaaatca attgcgcctc cgctagcttc tgtcaaacca
     8821 acatggaaat ctccttcatt caatacatgg atgccctctt cttcattatg taccttcaaa
     8881 ccaagtacat gattaagaaa ttggcgttct tttgttaagt tagtaacatt taaatggatt
     8941 ttttcaaaat aagaacctgt tgatagttta ctgaattttt cttcagattt tcttaataat
     9001 gcttcttgat ccattgtttc tggaatttct ctttggtcgt tcttattctc ttcatggtaa
     9061 atttccaatt cattctcttc tgggtctacg agcaaaatcc ccatacgtcc atgatcttct
     9121 aaagcttctt tgattggata attcatttta cggatacgac aaaggatatc tgccatttct
     9181 tctgtgctag ggataatcaa tgaaaaacgg ttcagctttt tgatcacccc catgtgatca
     9241 ttcgctctag gactttcttc cagcagcaac atttcacttc cagcttcttc tgttcctaaa
     9301 atggctaatt cgttttcttc tcttttcaaa tcaaagccaa caacgttctt gtaaaaatca
     9361 atcattttat ctcggtcttt cacccgaatg gctacacttt ttaaataggt ggcagccccc
     9421 aattgaaaat ctttcatatt tcttgctccc ctctacgtca agctttattt taacgatgga
     9481 ttcttcttct ttcaagcaat aactttgatt ttgttaaaaa aaatgtgaga aaattcgcat
     9541 tttgcttgtt ttttgtaaat taatgagtaa aaactcttat aaaaagaggt tgtgaaaaag
     9601 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
     9661 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtaaa
     9721 atcagcggtt tgacctgagt attagagata caaaacggaa aaatagcttc tcatattttg
     9781 ccgttttttg agctaatacc gaaggacagc tgattgaaca ccgtttattc ggaaaaaggg
     9841 actgtgacac gacttttgtc acagccccta aaaaaaataa taaatttgta ctattctatg
     9901 aagcttcttc ttcagaaaac tgactgttat aaagatctgc atagaatccg tctttttcca
     9961 tcaatgtatc gtggtttcct gtttctacga tcgaaccatg attcatcaca ataatgttgt
    10021 ctgcatcgcg tattgtagac aagcggtgag caacaacgaa acttgtccgg ttttccaata
    10081 ggcggttcat cgctttttga atcagtattt ctgttcgtgt atctacacta gatgttgctt
    10141 catcaaggat caggacatct ggatttgcta agaatgcccg cgcaattgtg attagctgac
    10201 gctgcccttg cgaaatattg cttgcatcct cgtttaatac agtgttatat ccttctggca
    10261 atttacgaac aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt tgttcgtctg
    10321 ttgcatcgtc gttcccatat ttgatattgt catagatact tcctgtaaac agccaagtat
    10381 cttgcagtac cattgaaaac tgtgcgcgca attcatcacg tgaaagatca cgggtatcta
    10441 caccatcgta acgaatacta cctccagaaa catcgtagaa ccgttcaaga aggttgatca
    10501 gtgtcgtctt ccctgcacca gtcggaccta caatagcgac catctcacca ggtttgacat
    10561 ccaagttaaa atcagtcatt agcaagttat ctggtgtgta gccgaattga acattttcaa
    10621 aacgaacttt gtatggtgta tctttttctt ctggaaggcc agatttttca ttggacatct
    10681 cttcttcatc caacacttca aatacccgtt cggctgaagc aactgttgat tggatagtgt
    10741 tcatcaaact tgcaagttga gtgattggtt gagagaactg tgttgtatat tgcaagaacg
    10801 cttgaacatc ccctaatgtc atgttcccat ttgcaacttt tactccgcct agtacagcga
    10861 cgaacacata gcctagattt ttaacgaaat tcatcaacgg catgatgatt gccgaaataa
    10921 attgagcttt ccaaccagcg gcatacagat tctgattttc tttttcaaat acttcctggt
    10981 ctttttcagt atgattgaat gttttaacaa tggtatgacc accatatgtt tcttctactt
    11041 ggttattcaa taaacctaaa cttttttgct gatctgcgaa aaacacttgt gatttcggcg
    11101 ctacgatcat cacgatgatc aagcttaaag ggatcatcaa taacgcgact aacgtcaatt
    11161 gccagctgat tgaaagcatc atcacgatga ccccaatcaa tgtgactgca ctagttacaa
    11221 attgagtcaa attttgctgc agcgtgcttg cgatgttatc catatcattg attgctcgag
    11281 acatgatatc cccatttgta tgtgtatcat aataggaaat cggtacacga ttcattttgg
    11341 cttccaaatc atgacgtaat tcataaactg ttcgttggga aacacgtgtc ataataaatt
    11401 gctgtaaaaa gttgaataca gcagaaatca aatacatcac gattactgtc gcaatgatct
    11461 gcgcgatttt atcaaagtct atcggaaatt ttgttacttg ttggccttgt tgcatctgtt
    11521 gagcgccagc cattacacct ttgaatattt ctgtggttgc tttccccaag acctttggtg
    11581 tttggatttg gaagatcacg gctgctattg ctagtacaaa tacagcgatg atcgcaacca
    11641 tacgtttaga catatagcca aacaatcggc gtaccgttcc ccagaaattt ttaggctttt
    11701 caccttttac accgagattt ctgccaggtc caccatgtcc tggaccgcct gctggtccat
    11761 gagagggacg tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt gtgaatgaac
    11821 gatttcttga taagtttcgt ttgttgctaa tagttcttca tgtgttcctt tgccaaccag
    11881 ttttccttca tctagcacaa ggatcatatc tgcatccatt actgtactta ctcgttgagc
    11941 aactaaaacg gtgatacttt ctttcatatt cgttttcaat gcttggcgaa gattggcatc
    12001 cgttttgaaa tccaacgctg agaaggaatc atcaaacaca tagatatctg ctgttttgac
    12061 taatgcccgt gcaattgcca gacgctgtct ttgtccacca gagaagtttc ctcctccttg
    12121 ttccacatgg ctatctaagc catcttccat ctctgaaaca aaatctttag cttgtgcgat
    12181 ttctaatgct ttccaaatca tttcatctgt tgcatccggc ttaccatact gcatattgtc
    12241 tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca aagcccatta attctcgcaa
    12301 attatattga gtcatgtcac taatgtccgt tccatttatt tggatcgaac cagattcgat
    12361 gtcatataat cgagggatca aattgactaa ggtcgtttta cctgaaccag tcccccctat
    12421 gattgcgacg atctcgcccg acttggcttg gaaatcaata tcttccaatg caagtttttc
    12481 tgctccatga tatcgataat tgacatgatt gaaggccaat gttgcgtttt ttccagcaaa
    12541 agaaactgtt ttaggatttt cagggtcatg gataccgatt ttttcatcca acacttcatt
    12601 aatacggtca gctgaagctt gtgcacgagg caccatgata aaaatagcgg aaagcatcat
    12661 aaagctgatc aagatctgca tcgcataagt cataaatgca atcagattac cgacttccaa
    12721 agtcatatct gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg taccgctcat
    12781 gatcaatgtc atcattggca tcatcaatgc gacgatcgta ttaactttga ttgccgtatt
    12841 tgtataatct ttattcgcta aatcaaaacg attttcttca aagcgtgtct tatcgaatgc
    12901 tcgaatcact ctgacccctg tcaatccttc tcgaaatacc aggtttaaac ggtcagtttt
    12961 cttttgcata cttttgaata atggaacagc aaagtacatg attccgccga tcaaaataat
    13021 cattactggg atgacaacta agaagatttt tgtcaactga tgatcttttt gataagcaag
    13081 tatgctcgcg ccaatcaacg tgattggtgc attgatcatc atacgtaaaa acatttgtgt
    13141 aaccatttgg atttgagtca catcattggt cgttcttgtg atcaaagagg ccgttccata
    13201 tttatcaaac gcatcttttg tcagactctc tgatttttta tagacatccg tacgcaattg
    13261 cttacccagt ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag cagctaagat
    13321 actgatcaaa gaaaagccga tcatcacaaa acctgtatgc caaatataat cgacatctcc
    13381 ttgcgctacc cctttatcaa taatattgga ggtaagtgtc ggcaaatata aatctgccag
    13441 tacttggatc accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga tccgttttac
    13501 gatttttagc atctagttcc cttctttcta tttatatact ccgtttttca gccaagaaag
    13561 tttcaaattg aattcactga ttgcttgctc tactgagtaa tcttcagatg ttgaaaataa
    13621 gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc actaattgga tcagtaattc
    13681 caattcatga tcatttttta ctttcagctt cgtccgatcg atcaattcaa gcaactcttt
    13741 tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac tcgtatgagg
    13801 agcgactttt cttgaggcac ggtaatccat gttcataaac aagttacgat aaaaagaagc
    13861 attctcgcct tccaaaatct cataaatcat tttagaaaaa taggattcga atcctttgaa
    13921 tagatcacca tcggattctt tcattagttg taacatatct ttagagctgt cacgtctcat
    13981 tgtctcaaag taataaaaat aaagatcctc tttatcttca aaatactgat agaaactgcc
    14041 tctagggata tccgctaatt tgacgatatt agcaatcgaa gcttctttta agggtactct
    14101 agaaaactcg attctcgcag cctcgattag tcttttttgt ttttctttag gcaaatggaa
    14161 aaatgtctgt gtcggcatat ggatcctcct ttctttcaag atgacacatt gtcatatgac
    14221 gctgtgtcat tataacacct cctacctgtt ttgcaagaca tttgcatagt ttttttcatt
    14281 atcatccgat acattaaaaa agtgataaga ggaatccttc catttgaatt aatgagagtt
    14341 gcccattaaa atagccacat actaaacaaa ggatgagata aatggcccaa tcaattagtg
    14401 tccaagactt caataaattg cctctcgatg ctgacagcat cgttttagat acaagaaata
    14461 ccttagatta cacaaaaaac cacttaagcc actcgctttc tatccccgct gctatcttgc
    14521 caagacaatt aaaaaatcta gatccaaaga aactttatta tattctaagc tatacaggca
    14581 ggcgatcaga agcattagcc gatcaactta ctgctaatgg ttttcgtgct gtttatatca
    14641 ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt agattcaaaa
    14701 gaggctgtga caaaaaatcg tgtcacagcc tcttttatac cctgtctttt tttagttatt
    14761 tattcttcaa ccttatcaat atgacttgat agccttttct ttttctcata gttataacaa
    14821 tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc aaaggtcatc
    14881 ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta acaagttata
    14941 aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat gatattccgg
    15001 aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca gaattcactt
    15061 ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact gatcagttcc
    15121 ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc ccaacaaaat
    15181 taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat acagaaaacg
    15241 ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat ggcggtgtat
    15301 acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt gtaggcatcg
    15361 atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt gcagatgagt
    15421 tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa aaattgagat
    15481 aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgta ccttggatat
    15541 tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt cttcataaag
    15601 agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag ctgctgcata
    15661 aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag tcagcagaac
    15721 taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg gagagatcaa
    15781 gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga tcatgaatgg
    15841 acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc ctaagtaagg
    15901 gatcaggttc aaaaaacccg caataatccc caacgtgaca gcatactcta aaccaatgac
    15961 cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt gtcctcttat
    16021 ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt tttgcatgcg
    16081 tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca atagataaaa
    16141 taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc cgacaaaact
    16201 tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg ccgaattcat
    16261 gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa
    16321 aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac tgatgaagct
    16381 cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga ttagtgcaac
    16441 aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct tttcaaagta
    16501 atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag gcagacctac
    16561 gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac
    16621 gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat ttaaaaacca
    16681 gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa ttcctcctgt
    16741 tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg atgaagatag
    16801 attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga tacgtgagct
    16861 actgtctcta aattcccatt ggccatcgga ccgacataat cgatcgtata tacctgatca
    16921 tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga
    16981 atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat cagcatgggt
    17041 tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat cattgcactc
    17101 tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc cgtgtggaat
    17161 aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga atcagctcat
    17221 atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg aatctatacg
    17281 atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt tgaagaaaat
    17341 agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac tagtgtggat
    17401 ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc ggtaacagag
    17461 tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact atacggggcg
    17521 aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga actgaaagca
    17581 gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat
    17641 gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct tggtacagat
    17701 cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac taccttcact
    17761 gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca attcgcttat
    17821 ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga aacaggtgct
    17881 tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga aaatggaggg
    17941 gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg aggacataac
    18001 tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc
    18061 tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa ttttgtcgtg
    18121 tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga aacaggatta
    18181 ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt
    18241 tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact acgggaagaa
    18301 ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga agaggaatat
    18361 cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa tgtagaaaac
    18421 taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc gtctatcctg
    18481 atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga aaaggagtat
    18541 ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca ttatcataaa
    18601 ggattttgat cactctatca taaaggtaat attacattta tattagataa ctctagtttt
    18661 tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt tttggatggc
    18721 agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga atagatcttc
    18781 atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg ctacatatga
    18841 aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg gacgcaaagt
    18901 actgactttc ttaagcattg cccgatattt ttttgttcct tcttcaaata acaggatgat
    18961 ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg cgtttctgat
    19021 cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga cgatttgatc
    19081 gatggactgc acataatagc caatattcgg taacatgttc gttttcctac tttctccatt
    19141 gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg aaaaaattta
    19201 atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct gctaaactat
    19261 attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa atctcttgtt
    19321 gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac agttctcgtt
    19381 caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca tttgttgtct
    19441 gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag
    19501 tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt tcatgcaatg
    19561 tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga tgatcccccc
    19621 aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg
    19681 ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc ccacttccat
    19741 ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc atatgtgtct
    19801 gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt gtcattactt
    19861 ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc gtataggtca
    19921 tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc tctcttttat
    19981 acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg tttaacgctg
    20041 aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt
    20101 gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata tttgctgttc
    20161 caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct acttcataat
    20221 aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga tctgagaaac
    20281 tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta tttttttcat
    20341 cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa tatccttcag
    20401 gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa ccagtgttat
    20461 agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat gcttttcgca
    20521 acaaattatc gggattgttg aaattactga tataccctag cagtccaatt gccacaagca
    20581 gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga aacatgtacc
    20641 agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac agcatagtgc
    20701 cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct ggataaatcg
    20761 gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg atggctacga
    20821 catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa ataagaataa
    20881 tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca
    20941 acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta ctaaaaacaa
    21001 caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc tgtttggtcg
    21061 aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa taaaagaatg
    21121 ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat
    21181 gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag cggacctttc
    21241 gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca aaatcagtac
    21301 tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat atatctgcga
    21361 tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg gttttctttt
    21421 gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat ggataattga
    21481 ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact gccaaacgtc
    21541 cgctgtctgc caaagcttcc tgagtcgatc cccctagatg aggcaacata agtatacggt
    21601 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
    21661 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
    21721 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
    21781 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
    21841 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
    21901 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
    21961 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
    22021 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
    22081 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
    22141 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
    22201 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
    22261 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
    22321 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
    22381 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
    22441 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
    22501 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
    22561 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
    22621 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
    22681 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
    22741 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
    22801 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
    22861 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
    22921 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
    22981 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
    23041 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
    23101 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
    23161 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
    23221 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
    23281 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
    23341 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
    23401 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
    23461 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
    23521 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
    23581 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
    23641 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
    23701 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
    23761 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
    23821 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
    23881 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
    23941 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
    24001 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
    24061 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
    24121 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
    24181 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
    24241 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
    24301 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
    24361 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
    24421 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
    24481 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
    24541 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
    24601 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
    24661 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
    24721 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
    24781 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
    24841 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
    24901 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
    24961 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
    25021 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
    25081 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
    25141 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
    25201 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
    25261 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
    25321 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
    25381 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
    25441 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
    25501 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
    25561 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc gtttggctac actgttcgta
    25621 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
    25681 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
    25741 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
    25801 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
    25861 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
    25921 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
    25981 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
    26041 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
    26101 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
    26161 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
    26221 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
    26281 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
    26341 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
    26401 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
    26461 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
    26521 aagcggttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
    26581 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
    26641 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
    26701 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
    26761 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
    26821 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
    26881 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
    26941 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
    27001 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
    27061 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
    27121 tatttgctaa aagtgttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
    27181 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
    27241 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
    27301 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
    27361 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
    27421 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
    27481 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
    27541 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
    27601 actttattga aaataggcgg tagcgtgatg ggactttcat tcatcctacc agctgtttta
    27661 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
    27721 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcatagg cgaaatattc
    27781 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
    27841 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
    27901 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
    27961 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
    28021 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
    28081 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    28141 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
    28201 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
    28261 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
    28321 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
    28381 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
    28441 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
    28501 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    28561 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    28621 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    28681 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    28741 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    28801 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    28861 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    28921 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    28981 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    29041 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    29101 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    29161 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    29221 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    29281 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    29341 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    29401 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    29461 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    29521 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    29581 tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt
    29641 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    29701 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    29761 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    29821 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    29881 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    29941 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    30001 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    30061 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    30121 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    30181 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    30241 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    30301 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    30361 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    30421 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    30481 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    30541 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    30601 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    30661 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    30721 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    30781 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    30841 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    30901 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    30961 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    31021 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    31081 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    31141 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    31201 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    31261 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    31321 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    31381 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    31441 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    31501 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    31561 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    31621 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    31681 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    31741 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    31801 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    31861 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    31921 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    31981 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    32041 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    32101 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    32161 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    32221 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    32281 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    32341 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    32401 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    32461 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaaattt agtctcaact
    32521 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    32581 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    32641 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    32701 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    32761 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    32821 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    32881 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    32941 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    33001 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    33061 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    33121 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    33181 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    33241 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    33301 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    33361 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    33421 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    33481 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    33541 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    33601 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    33661 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    33721 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    33781 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    33841 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    33901 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    33961 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    34021 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    34081 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    34141 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    34201 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    34261 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    34321 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    34381 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    34441 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    34501 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    34561 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    34621 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    34681 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    34741 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    34801 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    34861 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    34921 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    34981 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    35041 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    35101 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    35161 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    35221 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    35281 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    35341 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    35401 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    35461 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    35521 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    35581 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    35641 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    35701 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    35761 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    35821 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    35881 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    35941 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    36001 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    36061 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    36121 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    36181 tgtttctgtc ctgattggtg caataacgtt taccaattga gcaagacaag caatctttac
    36241 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    36301 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    36361 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    36421 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    36481 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    36541 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    36601 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    36661 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    36721 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    36781 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    36841 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    36901 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    36961 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    37021 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    37081 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    37141 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    37201 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    37261 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaag
//
LOCUS       DANKAY010000014        36643 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000014 DANKAY010000000
VERSION     DANKAY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            23..3301
                     /locus_tag="PWY98_000734"
     CDS             23..3301
                     /locus_tag="PWY98_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2036508.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3842..5749)
                     /locus_tag="PWY98_000735"
     CDS             complement(3842..5749)
                     /locus_tag="PWY98_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2036509.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5879..6754
                     /locus_tag="PWY98_000736"
     CDS             5879..6754
                     /locus_tag="PWY98_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2036510.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6834..7808
                     /locus_tag="PWY98_000737"
     CDS             6834..7808
                     /locus_tag="PWY98_000737"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2036511.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7988..9133
                     /locus_tag="PWY98_000738"
     CDS             7988..9133
                     /locus_tag="PWY98_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2036512.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9270..9614
                     /locus_tag="PWY98_000739"
     CDS             9270..9614
                     /locus_tag="PWY98_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2036513.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9885..11171
                     /gene="efmA"
                     /locus_tag="PWY98_000740"
     CDS             9885..11171
                     /gene="efmA"
                     /locus_tag="PWY98_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2036514.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11309..11908)
                     /locus_tag="PWY98_000741"
     CDS             complement(11309..11908)
                     /locus_tag="PWY98_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2036515.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12312..13433
                     /gene="mnmA"
                     /locus_tag="PWY98_000742"
     CDS             12312..13433
                     /gene="mnmA"
                     /locus_tag="PWY98_000742"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2036516.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13679..15099
                     /gene="arcD"
                     /locus_tag="PWY98_000743"
                     /pseudo
     CDS             13679..15099
                     /gene="arcD"
                     /locus_tag="PWY98_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
     gene            15113..16231
                     /locus_tag="PWY98_000744"
     CDS             15113..16231
                     /locus_tag="PWY98_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2036517.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16291..17583)
                     /locus_tag="PWY98_000745"
     CDS             complement(16291..17583)
                     /locus_tag="PWY98_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2036518.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17715..18293
                     /locus_tag="PWY98_000746"
     CDS             17715..18293
                     /locus_tag="PWY98_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2036519.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18422..18799
                     /locus_tag="PWY98_000747"
     CDS             18422..18799
                     /locus_tag="PWY98_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2036520.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18732..20804
                     /locus_tag="PWY98_000748"
     CDS             18732..20804
                     /locus_tag="PWY98_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2036521.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21015..21962)
                     /locus_tag="PWY98_000749"
     CDS             complement(21015..21962)
                     /locus_tag="PWY98_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2036522.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22208..22672
                     /locus_tag="PWY98_000750"
     CDS             22208..22672
                     /locus_tag="PWY98_000750"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2036523.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22734..23777)
                     /locus_tag="PWY98_000751"
     CDS             complement(22734..23777)
                     /locus_tag="PWY98_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2036524.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24154..25119)
                     /locus_tag="PWY98_000752"
     CDS             complement(24154..25119)
                     /locus_tag="PWY98_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2036525.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25353..26198
                     /locus_tag="PWY98_000753"
     CDS             25353..26198
                     /locus_tag="PWY98_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2036526.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26389..27576
                     /locus_tag="PWY98_000754"
     CDS             26389..27576
                     /locus_tag="PWY98_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2036527.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27582..27938
                     /locus_tag="PWY98_000755"
     CDS             27582..27938
                     /locus_tag="PWY98_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2036528.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27940..28278
                     /locus_tag="PWY98_000756"
     CDS             27940..28278
                     /locus_tag="PWY98_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2036529.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28354..28610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28673..29707
                     /locus_tag="PWY98_000757"
     CDS             28673..29707
                     /locus_tag="PWY98_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2036530.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29700..30386
                     /locus_tag="PWY98_000758"
     CDS             29700..30386
                     /locus_tag="PWY98_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036531.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30410..31258
                     /locus_tag="PWY98_000759"
     CDS             30410..31258
                     /locus_tag="PWY98_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2036532.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31455..32228
                     /gene="sufC"
                     /locus_tag="PWY98_000760"
     CDS             31455..32228
                     /gene="sufC"
                     /locus_tag="PWY98_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2036533.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32241..33527
                     /gene="sufD"
                     /locus_tag="PWY98_000761"
     CDS             32241..33527
                     /gene="sufD"
                     /locus_tag="PWY98_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2036534.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33524..34759
                     /locus_tag="PWY98_000762"
     CDS             33524..34759
                     /locus_tag="PWY98_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2036535.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34746..35216
                     /locus_tag="PWY98_000763"
     CDS             34746..35216
                     /locus_tag="PWY98_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2036536.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35221..36612
                     /gene="sufB"
                     /locus_tag="PWY98_000764"
     CDS             35221..36612
                     /gene="sufB"
                     /locus_tag="PWY98_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2036537.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        11973 a         6776 c         7861 g        10033 t
ORIGIN      
        1 atttgttacg ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat
       61 gaatctcctt atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga
      121 acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga
      181 ggaaatacca agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa
      241 agaatcagaa aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg
      301 ggttgttttc ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg
      361 cgtgcggctc tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat
      421 gacctattat tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga
      481 aatagaatct tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc
      541 agtaccaaaa aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg
      601 cagtcggatc gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag
      661 caaatatacg atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga
      721 tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc
      781 agcagggttc ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa
      841 tctgtttccg gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac
      901 tggtttagag gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat
      961 gtatgactgg gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc
     1021 agcaaggaat atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac
     1081 agatcctcct gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc
     1141 tagtaatcat tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt
     1201 attgacaatt aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat
     1261 cactgcgaat cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac
     1321 tttcagtttt acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta
     1381 tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta
     1441 tgtaacacca gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac
     1501 acaagcattg agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc
     1561 cttcgaaaga ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca
     1621 gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg
     1681 aaatgggatg acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag
     1741 tgcagctatg caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca
     1801 tgcttatcgg aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga
     1861 agcatggccc gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg
     1921 atttgaagat gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga
     1981 cggttgtatc gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac
     2041 aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc
     2101 aggaattgag ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg
     2161 ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa
     2221 tagcggaaca gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt
     2281 ggctctttat acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt
     2341 ccgaacagag aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca
     2401 aatctaccac tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct
     2461 tggacatacc atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca
     2521 gcagcctgtt acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa
     2581 accggggaat cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc
     2641 tatttttgac ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga
     2701 accaaatcag acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg
     2761 tgtggcggag aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc
     2821 aactttcaca ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa
     2881 gatccaaatc gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc
     2941 agcaccttca aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg
     3001 ggaaaaatgg tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga
     3061 aactgccgat gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc
     3121 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3181 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3241 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3301 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3361 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3421 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3481 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa
     3541 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3601 taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3661 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta
     3721 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3781 agaagcgatt ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     3841 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     3901 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     3961 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4021 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4081 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4141 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4201 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4261 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4321 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4381 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4441 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg
     4501 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4561 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4621 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4681 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4741 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4801 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     4861 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     4921 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     4981 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5041 ccgtgacaag ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt
     5101 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5161 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5221 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5281 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5341 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5401 gtaatgtatc caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg
     5461 attcaacttt ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat
     5521 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5581 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5641 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5701 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5761 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     5821 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     5881 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     5941 agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt
     6001 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6061 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6121 gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat
     6181 tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt
     6241 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6301 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct
     6361 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6421 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6481 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6541 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6601 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6661 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6721 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6781 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     6841 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     6901 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     6961 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7021 aatcaacgaa tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa
     7081 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7141 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7201 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7261 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7321 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7381 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7441 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7501 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7561 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7621 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7681 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg
     7741 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7801 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc
     7861 tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     7921 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     7981 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8041 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8101 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8161 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8221 taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac
     8281 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8341 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8401 agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa
     8461 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8521 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8581 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8641 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8701 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8761 tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     8821 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     8881 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     8941 taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca
     9001 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9061 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc
     9121 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9181 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9241 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9301 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9361 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9421 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9481 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9541 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9601 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9661 gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca
     9721 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg
     9781 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
     9841 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
     9901 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
     9961 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10021 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10081 gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10141 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10201 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10261 tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga
    10321 atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg
    10381 ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt
    10441 ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag
    10501 ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag
    10561 ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca
    10621 gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag
    10681 tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa
    10741 catggaaaga ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg
    10801 gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg
    10861 caggagcagg ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga
    10921 gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg
    10981 caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa
    11041 tgttcttgtt ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag
    11101 ctgcccgaaa ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa
    11161 aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc
    11221 ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt
    11281 gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat
    11341 agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga
    11401 gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat
    11461 gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat
    11521 gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa
    11581 atttgggact tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc
    11641 gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca
    11701 aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa
    11761 catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat
    11821 ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa
    11881 agtgcatatc actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa
    11941 acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg
    12001 tgcagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta
    12061 ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc
    12121 tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag
    12181 aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat
    12241 gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg
    12301 atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg
    12361 tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat
    12421 tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata
    12481 aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa
    12541 aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac
    12601 ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa
    12661 tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa
    12721 acggcgtggt ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta
    12781 gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat
    12841 cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa
    12901 cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag
    12961 caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga
    13021 tgtattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag
    13081 aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt
    13141 tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga
    13201 atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg
    13261 ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg
    13321 tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg
    13381 gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat
    13441 taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag
    13501 ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa
    13561 agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa
    13621 aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat
    13681 ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc
    13741 aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc
    13801 agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa
    13861 tcatttagga gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc
    13921 atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg
    13981 aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa
    14041 aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt
    14101 attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa
    14161 attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt
    14221 cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg
    14281 aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat
    14341 cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc
    14401 gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg
    14461 cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa
    14521 aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat
    14581 tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga
    14641 cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa atgccccaac ttattcgtta
    14701 gttgttactg caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc
    14761 tatcaatttg cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg
    14821 atctatcaag tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg
    14881 attgctgtag tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta
    14941 tgtagtattg cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa
    15001 catcaaatta cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt
    15061 tcaatcgggt tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat
    15121 cgcaaatttc ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat
    15181 tgcacaaagc tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag
    15241 cgtaagtaaa tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg
    15301 atcagatgtt ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat
    15361 cttacaaacg aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc
    15421 aggagaccat gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc
    15481 tttaggtgcc gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt
    15541 agaacagcta gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa
    15601 caatcctaca ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg
    15661 tacagtagat gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga
    15721 attcttgtcg atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac
    15781 ctactccatt ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt
    15841 ttttcgaaag tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc
    15901 tgtacatgca ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga
    15961 gaaccttgtg attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc
    16021 caattacgtc tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg
    16081 tatcaattta ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc
    16141 gaagcatgcc cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct
    16201 cttatctgag ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac
    16261 aactgttcaa gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga
    16321 agctatggag actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt
    16381 attgtgtcgt acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc
    16441 gcgataacca aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt
    16501 tgcttggaaa gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt
    16561 tgtgatcaat gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg
    16621 taaggcagct aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc
    16681 aaaacccaaa ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa
    16741 tcttggagcg gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa
    16801 cgtaacattc ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat
    16861 ggaattgttg gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg
    16921 atatttcaag tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac
    16981 aaatttttca tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt
    17041 aatgaaaaag gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc
    17101 aagtaatgtt cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc
    17161 ttttggcgtc ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc
    17221 ttgaagcaga cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat
    17281 gacgattgcc attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc
    17341 taattttccc caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa
    17401 tccgctccaa aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga
    17461 cataaaaggc atgactaaac taaatccgat accagccata aatgttccaa accataagac
    17521 aaacaagttt tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc
    17581 caattagact ccttctttct gaaaattttc atctacagct ctttattata aactacaata
    17641 tgtagtttat caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt
    17701 gaaggattta ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga
    17761 aaatcatcga ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc
    17821 agcatttagc caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata
    17881 ttcgtgatgt caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc
    17941 gacgggctct tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc
    18001 gatcctttgc tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca
    18061 atgaagctgt tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg
    18121 aggcttttca attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga
    18181 tccacggtta tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc
    18241 cagaagacag ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca
    18301 gattcatttg taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct
    18361 tgcaactttc ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag
    18421 tatgtatcaa gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg
    18481 ggaagaagat attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta
    18541 cgaaaaacaa tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt
    18601 tctcagcgcc ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt
    18661 acagcaatac aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag
    18721 aaaggaattt tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa
    18781 gcaaggtgct tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg
    18841 ttacttgggc ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat
    18901 ggcagagctg gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac
    18961 ttcctaaaac aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct
    19021 cagaagcagt cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct
    19081 tagagtatgt gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata
    19141 aagcaatcaa aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg
    19201 aatctgctct tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct
    19261 taactgtgaa gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat
    19321 tagtgagaga ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca
    19381 cgttgcaagc tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa
    19441 cacaagaaga agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag
    19501 aagtccaaga aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc
    19561 aaagagaagc caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa
    19621 gtttgtatga gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa
    19681 atgacatgaa aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg
    19741 tcaagcagta tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta
    19801 aagtgaccga tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa
    19861 gtaatgaaga gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc
    19921 aaccccataa agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt
    19981 ttcggcaaaa tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa
    20041 cagtcaaatg ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat
    20101 ctgcaccgcc taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc
    20161 tgggaacccc agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg
    20221 aacgtacccc tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa
    20281 atccctcttt agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg
    20341 ctgcaggaaa gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct
    20401 tggtgaatga gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga
    20461 aatgcccaga atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg
    20521 tctgcaccaa ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc
    20581 gatgcccgca atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc
    20641 gttgtaaata cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa
    20701 tgaccaaaca tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag
    20761 aaagcccatt agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg
    20821 ttgtgataga agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa
    20881 atttttgtga aatttcggct tatttccgaa gaagctactt ctggaacacc atttattcgg
    20941 aaaaaaggac tgtgacaaga cttttgtcac agtccttttt catttaaaaa aatgtttact
    21001 tcatttgacg tatattattg gtgaggcttc aattcatttg gcttcacttt ttctgtttct
    21061 cttatgatat aaacaggacg ctgtttcgtt tctaagaaga ttttcccaat atatttcccg
    21121 atgattccaa gacataacaa ttggattcca ccaatgaaca ggaagatcga gaccatggaa
    21181 ggccatcctg atgtaggatc accaaacaac aatgcccgca aaataatgaa caaaattgca
    21241 ataccagaac cgacacaaga caaagcgccg ataaatgaag caatcgttaa cggtgcatcc
    21301 gagaagttca cgataccatc aatcgagtag ttgaataatt tccagaagga ccaagatgtt
    21361 tcgccagcca cacgttcgcg gttttcataa gaaatatatt ctgttttaaa tccgacccag
    21421 ctaaataatc cttttgaaaa tcggttgtat tctgttaatt ctaggacggc atccaccatt
    21481 tgacgagtca tcaagcggaa atcacgagcg ccatcgacca tttctgtatc gctgatttta
    21541 ttgatcaact ggtagaattt tttcgcaaag aaactgcgta tcgcaggctc gccttcacga
    21601 gtcgaacgcc gggtgccgac acaatcgata tcagaagtag tgatccggtt gatcatctca
    21661 ggaagcagtt cgggcggatc ttgcagatca acatccatca cggtcactaa atctcctgta
    21721 gaagcttgca atccagcgta cagagccgct tctttcccaa aatttctgga aaatgagaca
    21781 aagcggacat aaggataagc tgaggctagc ttcttgattg ctgctaacgt accgtctttt
    21841 gatccgtcgt caataaacaa atattcgaac tcatgggcac ctttgaattt ttcaacctca
    21901 tcaaaaaaaa gaggaacagt tttttcttca ttaaaacatg ggacaacaat tgatactttc
    21961 atcgtttcta tcctttcaat cgtatgttta tatatggttt acattcaaaa tttaataaac
    22021 cttaaataac acctttaaaa tttgatttta cgtaaaagaa caggtattct ttttgtaaac
    22081 taacgtattt agtttaccat gtttcctcgg tttatcaaac ttttaagaac aatttttatt
    22141 agttggcaag tagttttgga tagagaaaaa ggaggaagtt ccagtctatt gactaggagc
    22201 aaacaaaatg aaaatacaca cacctatcgg ctttttatgg ttagatgcag atgaatccgg
    22261 attgactgct gtatcttttc agccaatttc agaaagcgaa tcagcgaatc agcgaatttt
    22321 aacgcgcaca caagaacagt ttgaagaata ttttgcggga aaaagaaaag aatttgatgt
    22381 tcctttatct ataaagagag gtacgccttt tcagcaaaat gtctggaaag ctttgtcgac
    22441 cattccttat ggagaaatcc gaacgtataa agaagtagcc gttgctgttg atagtccgaa
    22501 agctgtgcga gcgattgggc aggcaaatcg aaccaatcca ttgccaatcg ttattccttg
    22561 tcatcgagtg atcgggcaaa atggaaaact gacaggatat atgggaaacg caatagatgg
    22621 ggttgaaatc aaacgccaat tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt
    22681 gtgattcgga cattgttcca tgcgagaatc acaactgttt tttcatgttc attctaaact
    22741 ttgatcagcc aaataaatag taaagccatc actgtttgga gaagcccgac agctaatcca
    22801 taagataaga agcgtgtacc ttctttcatg aattttgcaa agtctaaacg cagaccaatt
    22861 gccgctaaag cagtgatctc aaaccagctg ctgataaaat gtgccgattg gtcaataact
    22921 acaggtaaag aaaacaagct gttgaccaaa caaaagatca caaaccctaa tacataccaa
    22981 ggaagcggta attttcgttt tttctgcgta tcagttggcg tttcaaacag agctggttgt
    23041 ttcttgtgca tgaatttttc aaacagatag acaacaacaa cgagcatcat aatacgtaga
    23101 atcttaaata gcatggcgaa ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact
    23161 acttgaccaa cagattggag tgtaccaccc aaaagtgcac ttttttcaat gagatttcca
    23221 ccaaacaact gtaatccaag aaaagggaga gtcaacatca tcacagtacc cagtagattg
    23281 actagtgtga tgatctgtcc tttttcttcc tctttcgcat ggatggaagg ggcgattgaa
    23341 gcaatagcag aagaaccaca aacagcgttt cctccagcca tcatcaaaga agaagtatcg
    23401 ctgaattgca gttttttacc aatcaagtaa gcaccgatga tagtgactga catttgcagt
    23461 aacacaaaaa caattccttt tacaccaatt tgagcaatcg tttgagctgt gacagttgca
    23521 cctagtaaaa aaacagagaa ttctaatagt ttgctttcag caaattttgt gccgattgct
    23581 agaacaggct gtttaaaaaa agtatttcct aataaaatac ccaataaaat agcaatagtc
    23641 gctccaccta actgtggcag ataaatagca agaagcctgc tgaaggcagc gatggcaaaa
    23701 gaaagcagta aaccgggtaa gatttcgcgg accgctgaat cctttacaga ttttattgga
    23761 tgtgacaatg atttcatttg atcggttcct ttcgtcaata aatactaaaa gcatactcga
    23821 acatagtagc actaaaccag tattttgaca aaagaacaag aggtcgtgac aaaagtcttg
    23881 tacgacctct tgtttcgaat aaacggtgtt ccagaagtag cttctacgga aataagccga
    23941 aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
    24001 cacttctttt acgacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    24061 ccaaccagta caaaaacatg aacagctgtt tttgtttggc cgttcttatt acttgatgca
    24121 gaacagcttt ttcacaacct attgaatcga ttattaagcg atttttttag aaacaaattt
    24181 tccatgtttg ctatgttctt taattaagaa acgtacgtaa tgagcaagaa cgtagaagac
    24241 aactacactt gcaaacagac attccgcaac ttttaagaaa tccaatacgc tactatagat
    24301 acctaaactg ttgaagtaat gaacagtagt aaatatagct gttgcagaga taacaagcgg
    24361 gaatgtaaag gctgcatagc ttggataaaa acgaagcttg aacaaacgtg gaagcataga
    24421 cagcacaacg aaatacaata cttgagataa agcgaacaat gctacgacaa gataaaaatt
    24481 aggttcagca aagtttttaa gataacctgt taggcaaaga gaaccaggtg ctgcaacgat
    24541 cgtaatcagc ggacgtgtcg gatgagccat atttcttacg aaaaagacac gtttgatgat
    24601 gatcggcaat aagaacgcat agcagatcaa tgaaatccaa aagaaaattt ttccaataag
    24661 tgggaagaag ttcccggcag ttacagtgat gaccccaaat ccaacgtaaa tgataaacca
    24721 gcttggataa attgcttcaa ttgaaagatc agctttgata acatggtgat aattgaaata
    24781 aaagacaagg atcacttgga agataacagc gcacagccaa atgaatttga caattggtgc
    24841 aatactttca tatgaaacaa aataagtaca aataaccatg agtgacatcg taaaagttgg
    24901 tgaaacggag gcgatgattg gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa
    24961 caatttaaca aaaatgaata tcataagaat catagagata ccgccagtta gattgcctaa
    25021 agcaacatgg tgatagtctt taaacagatt tccaagggaa gctaacccaa ggatcaaccc
    25081 gcagatcggg atcggaataa ttttcaaaaa gttcttcata tataatattc tcctttttat
    25141 ttaatagatt ttagtaccaa gatgaacgaa tacattgtaa aatgaatagg aatgtatgat
    25201 tacgagcaag cagacagctg attgtcttca tctaagttac tagttggatc agcaactgtt
    25261 ttgttgtaca tttctattat agatggtttt ttgtatattt aaatcaaata gtttttatag
    25321 taattatcat aaaaagttat ggaagtggtg ccatgtttaa gttattaacg acttttcgag
    25381 cagtatatga aacaagaaat ttttccagag cagcagaagt actgttttta tctcaaccag
    25441 cagtctcgaa tcaaatcaaa cagttggagc gggaattgaa tatcgatctt tttcaacgta
    25501 acggaagaaa agagatgctt ccaactaagc aagccgactt attatatgaa cggacactgg
    25561 ttttgttaga agaatgggaa gaaactcagc aaaaattgct caatgaacaa aatgaagaag
    25621 aagtatgtcg gatcgttgct tcccacacct ttgctgtcta tcttttgcct cagttgatga
    25681 aacatctgat caaaaggttt cctaaagtgc attttgagat cgaaatcacg aattctcatg
    25741 aatcattaga acaagtagcg aaacatgaat ccgattttgg ttttatcgaa aagccattag
    25801 agacttccgg cgtacaaaga tactctttga tggaagacca gctagtcatc gctggggacc
    25861 caaaaagcaa attgtggctt gtaagagaag caaactctgg tgtttttcat tacacgcagc
    25921 gttattttga agagatgaat atccaagata agaagatgat cgtgaaaagt aacgatgtaa
    25981 ttgttgcgtt gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc tctgttccag
    26041 aaaatgtgcc aatgactccg ttgaatttcc atcgatattt ttatttcatc caacgaacca
    26101 atctggtttc tgatcagctg ctagagattg ccgaagagat ccttcgatac aatgaaagca
    26161 cagaaacttc tcacagcgag gagaatctca gctggtagat aattgcatgt atcaaaaaaa
    26221 gttctatgat acactaaaga taagcagtag gcttttgtac tgcatttgct caagcaactg
    26281 cacaaacgga aagttaattt ttcccaaatg tctatggaaa gactttccat tatctaactg
    26341 gaccgctata ataatcagca gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa
    26401 ttaaaagctt cgaacgataa tcgtattgat tatggcgtga ttttaccagt ctttctgtta
    26461 tgtttgattg gtctgctctc attatatgta gcacttagcc atgatccgaa caatccagca
    26521 gtcttgaaag gcgtagggat gcaagttgtc tggtatttag ttggagcggc agcaatcgtt
    26581 gtcatcatgc atttgaattc caaatggatc tggaaactga ctccttacct ttatggtttg
    26641 gggttagtgg taatgggact cttgctcaaa ttctatgatc agaatttagc tgcttcaaca
    26701 gggtctaaaa actggtttcg attcggtgcg tttactttcc agccagcaga gttgatgaag
    26761 attgcgtaca tcttgatgat ggcactgatc gtaactaaac ataatacgca agtaaaagaa
    26821 cggacgatga aatcggactt ttggctgatt gggaagctct tgatcgtgac aataccggtt
    26881 ttagcattga tcatggccca agatgacttt ggaacgatgc ttgtctttct tgcgatcttt
    26941 ggcgggattt tcctgatgtc tggaatctct tggcgcatca tcgcacctgt agtaatcttg
    27001 gcagtagtcg ttggagcagg aacgatcttc ttggttacga ctgaaggcgg aagagacttg
    27061 ctttataaag ttggattcaa atcttatcag tttgcacgta ttgattcatg gctggaccct
    27121 ttccatgata cgtcaggaat gagttatcag ccggcccaag gacttttggc aattggtacc
    27181 ggtggcttgt ttggtaaagg attcaacgta agcaatatct atgtgcctgt tcgtgagtcc
    27241 gatatgattt ttaccgttat cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt
    27301 ttcttgtatt ttattttaat ctatcgtatg attcgggtgt gtttcgatac gaataatgaa
    27361 ttttacgcct atatcgcaag cggtttgatc atgatgctgc tgttccatgt ctttgagaat
    27421 attggagcaa atatcggatt acttccttta acagggatcc cattgccttt cattagtcag
    27481 ggaggatcct cgattttagg gaacatgatc ggtatcgggt tgatattatc gatgagatac
    27541 caaaatgaag cacctgcaag atcttcaagg aggtaagaaa attgtacacc ttttactggt
    27601 acccgaaatg ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt
    27661 atcaaaccgt tgatatgatc aaagaagtac cgaaggcctc aacgattgag acttggctag
    27721 aaacgagtca agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac
    27781 ttggactgaa agatcaagta ggcgagttta cggcttcaaa agcgagccaa gtcttatcga
    27841 cagatggtat gctgatcaag cgaccactga tcttacgaga ggaccagctt gttgcaattg
    27901 gattcaatga aaaaatttat gaaggagaac tgatataaaa tggcaggcaa gcatttgaag
    27961 aaaaaggaca acctttggat cttatttaat ggaaaagaat actgtgttgg tttgacaaat
    28021 gaagcgcaag aagaacttgg agacatcacg tttgccaatc tgccaaaagc aggacaatcg
    28081 ctaaaaaaag atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc
    28141 agcccgctaa cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc
    28201 ttgaatgatt cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa
    28261 gaatttgacg aattataaaa tcagaaaatc acttgaaaaa agttgacact gattcttcca
    28321 gttgctataa tcataagcaa tcctatatga aacgagaaag ctttgaaaag aagagtacat
    28381 gtcacggacg tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa
    28441 gatggtctta gagcagagta agcgagcgta agcaaactta ctacgggagt gcccgttaca
    28501 gcgcgacagt atacggaaag aagctaagtc caagtactgg aagaggctgt tattgcgaag
    28561 taacggtaaa tcaaggtggt aacacggaag ttcaagcttt cgtccttttg cttaaggcaa
    28621 gagggcgaag gcttttttgt ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt
    28681 aatccaacta acgaatgtca aaaagacatt caatgggaaa aacggaaaca tccaagccgt
    28741 caatgacgtt tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg
    28801 cgcaggaaaa agtacattag tccgtctcct gaatggactt gagcttccca cgagcggcga
    28861 agtgatcgta aacgatcaag atattacaaa attaaaaaac aaagaattac gtacattccg
    28921 aaagaaaatc ggtatgatct tccagcattt caacctgtta tggtcaagaa ctgtacttga
    28981 aaatatccag ctgccgctgg aattagctgg cgtaccaaag agcaaacgta aagaacgggc
    29041 agaagaactg ttgcgtttag tcgggttaga aggacgcgga caagcatatc cttcacaact
    29101 ttccggcgga cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg atcctgagat
    29161 cctgctttgt gatgaggcta ctagcgcatt ggacccgcaa acaacagaag aagtattaga
    29221 cttattgtta gcgatcaata agaaattgaa tttgaccatc gtcttgatca cacatgaaat
    29281 gaatgtgatc cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga
    29341 agaaggagac gtattgactg tcttcagaca accgaaaaaa gaagtaacca aacgctttgt
    29401 acaacaagac atcgaaccag aagctgattc gacagaacta ttagctgaac tcatcaaaga
    29461 aaatccaagc ggccgtttgg caactttgac ctttagcgaa agaaatgcca acgagccagt
    29521 catttcccaa gcgattcgac gctataacat cgatgtcaac gttgtgtatg gaaagatcaa
    29581 acaagtgaaa gaaggttcct ttggttcatt gactgtcttg atgactggtg aaaccaaaga
    29641 attagatcaa gcagaagtgt tcatcaaaga acaaggagta ggaattgagg tgatccatcg
    29701 tggataaaag cttttcagaa acatatctag attttaaaca aatcaacccg gaaacgatga
    29761 atcaggctat ccttgataca ctatatatga cattaatctc aatggtctta gtgacgatca
    29821 tcggattagt tttgggattg atcctttact ctattggacg caaaaacaaa ccgtatgctc
    29881 gaatcattta tggaatcgta tcgatcatca gtaatatttt ccggtcaacg ccatttatga
    29941 tcctgatggt tttgatcatt ccatttacta aagcaatcgt aggcactatg ctaggtgcgc
    30001 aagcagccat tccagctctt gttttatccg cagcaccatt ttatgcccgt ctagtagaga
    30061 ttgcttttcg tgaagtcagc cctggtgtac tggaagcagc agatgcaatg ggagcaagtt
    30121 actgggaaat catctggaag gtcttgatac ctgaaagtat tcccgcattg atttctggat
    30181 taacagttac aacgatctcg atgatcggat ttactgcgat ggccggcgcg atcggcgcag
    30241 gtggacttgg aggtcttgct tggcaggaag gctaccagcg tggtaatcta acagtgacat
    30301 tcgttgcgac tttaattatt ttactaatcg tatttatcgt tcaaggaatc ggtgatttct
    30361 taactaagaa aacagacaaa agataatagg atataggggg atgtaggata tgaaaaagaa
    30421 aatttttgga tttgcagcag cgcttttgct gactgtagga ttagcagcat gcggtaacaa
    30481 cagcgacagc aaagattcag caaataaagc agacgacacg acattgaaag tcggcgcttc
    30541 accgacaccg cacgcagaga ttttggaaca tgtgaaacca ttattgaaag atgaaggaat
    30601 cgatcttgaa atcgtcaaat tcgatgacta tgtattaccg aaccaatctt tggaagaagg
    30661 cgacattgat gccaattact tccagcatat tccttatttg aacaaagaaa tcaaagaaaa
    30721 aggatatgac tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa
    30781 aaaagtcaaa gatgtttccg aattaaaaga tggtgcaaca atcattactt caaattctga
    30841 atcagactgg ggacgcatca tcacaattct acaagatgct ggcttagtca aagtcaaaga
    30901 tggagtagat ctggaaacag caacattcga tgatattgaa gaaaacccta agaacttgaa
    30961 gttcaaccat acgatcgacc catcactttt agcttctacg taccaaaatg atgaaggtga
    31021 cctagttgca atcaacgcaa acttcgctta cggcgcaggg ttgaatccat tagaagatgc
    31081 cgtattgctt gaaaaggaca actctcctta cgtgaatatt attgcgactc gtaaaggtga
    31141 tgaagacagt gacaaaatca aaaaactgat tgaagtgctt caccgcaaag aagtccaaga
    31201 atggatcaca gacaaatggg gcggctctgt taaaccagta aaggctgatg caaaataagg
    31261 tatggaacaa aaaagatgag aaataagtct gtaatggctt atttctcatc ttttttatac
    31321 ctattctaat ttagaaatca aaattgttta ataataaata aaagatattt gattttagta
    31381 aggaaggtca ttgcgcttca ttctcaatta gtctaaaatg agtgagaacg aaagagaatt
    31441 tgggggtata atgcatgtct gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa
    31501 aagaaatcct taaaggcgta aacctaacaa tgaaaaccgg agaaatccat gccatcatgg
    31561 gacctaacgg cacaggaaaa tctaccttat ctgccgctat catgggcaat ccaaactatg
    31621 aagtaacaga aggcgaaatt ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg
    31681 aacgtgcgcg acttggctta tttttagcta tgcaatatcc aagtgagata ccaggtatca
    31741 cgaatgcgga attcatgcgt gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt
    31801 ctgttatggc atttttgaaa aagttagatg aaaaaatggc tttactaaat atgccagaag
    31861 aaatggctga acgttatctg aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga
    31921 tcctgcaatt gttgatgtta gaacctacat ttgcaatttt agacgaaatc gattcaggtc
    31981 tggatatcga tgctttgaaa gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt
    32041 tcggtgcatt gatcatcacc cactatcaac gattattgaa ctatatcaca cctgatgtcg
    32101 ttcacatcat gatggaaggc cgagtagtca tgacagggaa tgctgaccta gctaaacgtc
    32161 tggaagcaga aggctacgct ggtatcagta aagaattagg aatcgactat aaagaagaag
    32221 aagcgtaagg gggagcaaga atgaaagaaa aaaactggct cgattatctt gacgcggtaa
    32281 atgatttttc tttaagtaaa ggcgaacctg attggatgag aaccttccgt caagacgcac
    32341 tagcgaaagc agatgaactg ccattgccgc atatcgatcg ggtgaaattc catcgctggt
    32401 cgttatttga tgtgaaagaa acacaaacca tcagtgaaac aggcacgatt ccggcatttg
    32461 atgcaatgaa agacaatcct gtgcttgtcc aacaaggttc atggacgatt tttgaacaac
    32521 tgcctgtgga attagcagaa aaaggagtca tttttaccga cttgttcact gcaatgatcg
    32581 aatatccaga actagtccaa gaatattata tgaaaaaagc agtaaacatg aacgaagacc
    32641 aattgactgc actccatgta gcttttatga acagcggtat tttcctgtat gtgcctaaaa
    32701 atgtcgtgat cgatgaacca ctcgagtcct tattcattca agatggcgca tccgatgagc
    32761 acttcttcaa gcatgtgttg atcgtggcag atgaacatag tgaattttct tatctggaac
    32821 gtttccagac aaccaaagaa caagtagcaa aatcatcagg taatatcatc gtggaagtca
    32881 tcgctaaagc tggttcaaaa atcaaatatt cagccgtaga tcaattagga gaaaatatta
    32941 cttcttatat gaatcgccgt gggcacattt tacgagatgc ttctgtcgac tgggcaattg
    33001 gtgtcatgaa tgacggtcat gtgatcgctg actttgactc agatttagca ggagaaggtg
    33061 cacacgcaga agtcaaaatc gtggcaatca gctcgggcag acaaatccaa ggaatcgata
    33121 cacgagtaac caataaagcc cctcatacga tcgggcatat tttgcaacac ggagtgatcc
    33181 gagaaaaagg aacattgacc tttaatggta tcggccacat cttgaaaggg gcaaaaggtg
    33241 cagatgccca acaagaaagt cgtgtcttga tgctttctga taaagcacgt ggtgatgcga
    33301 atcctattct tttgatcgat gaaaacgaag taactgctgg acatgctgcc agtgtaggac
    33361 gagttgatcc agaagaaatg tactatctga tgagtagagg attgcataaa gaagaagcag
    33421 agcgcttagt gattcgagga ttcctcggtt ctgtcttaac agcaatccct gtagaacaag
    33481 ttcggaaaga attagtcgaa gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa
    33541 gctacgcaat gattttccta tcttatttca agacgtcaat gatgaacctt tagtttatct
    33601 ggataatgca gcgacgaccc aaaagccaaa acaagtactc caggcattag cgtactatta
    33661 cgaacacgat aacgccaatg ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca
    33721 tgattatgaa caagcaagag aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga
    33781 agtgctgttt actcgtggta cgacgacaag cttgaattgg gtagccagaa ccttcggcga
    33841 ccgctatatc aaagcaggag acgagatagt gctctcttac atggaacatc attctaatgt
    33901 tatcccgtgg cagcagttgg caaaacgaac aggtgctgtc ttgaaatata tcgaactaac
    33961 agaagaaggc ttcctggata tggaggatgc agcgaaaaag attacggaca aaaccaaaat
    34021 cgtctccatt gctcatgtat ccaatgtctt aggtgtcttc aatcctgttc aagaattagc
    34081 tgaacttgtc catgaaaacg gcggggtcat cgtagtagat ggtgctcaat ctgccccgca
    34141 catggccatt gatgtccaag cactagactg tgatttcttt gctttcagcg gacacaaaat
    34201 gtgcggacca acaggtattg gtgttttata tggtaaacga aaatggttag aagaaatgga
    34261 acctgttgaa tttggtggcg aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa
    34321 agaactacct tggaaattcg aagcagggac accaaatatc gccggcgcta tcgcattagg
    34381 ccatgcaatc gattatttgc aaaaactcgg tatggacaat atccatcgat atgaagaaga
    34441 actagccgct tatgtgttgc caaaattaca agcaattgat ggattgactg tttatggtcc
    34501 acaagacccg aattttcgga caggagtcgt tgcattcaat ttggatgggt tacatcccca
    34561 tgatgtcgcg acagctttag acatggaagg ggtagccgtc agagcaggtc atcattgtgc
    34621 gcaaccgcta ttgaagtacc ttgaagtatc agcgactgct cgcgcgagct tttatttcta
    34681 caacacaacg caagacgccg accgtttagt tgaagcaatc atggcaacaa aggagttttt
    34741 ccaacatggg actatctaaa ttagacaatt tataccgtca agtgatcttg gatcattctt
    34801 cccatccgca tcatcgtgga agcttgactg actcgacaag caagatcgaa ttgaacaacc
    34861 ccacatgtgg agatgtgatc gaattgcaag tcgatgtaaa agacggaatc atcaaaaata
    34921 tcgcctttga cggcagtggc tgctccatca gtacggcaag tgcctcgatg atgacagacg
    34981 ccgtcatcgg aaaatcaata gaagaagcag aacatttaac tgtagacttt ctacagcttg
    35041 tccaaggaaa agaagtcaaa gatcccgacc aattaggcga tgctgctatg cttggtggtg
    35101 tagctaaatt tcccgctcgg atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag
    35161 caatggaaaa cgatggtacc gcagctttcc atcattacca caaggaagag gagtgagaac
    35221 atgggcgtac cagaattaga agaatataaa ttcgggtttc acgatgatgt cgaaccggtt
    35281 ttcagtacag gagaaggctt gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc
    35341 gaaccagaat ggatgcttga attccgtctg aaatctctag aaacatttaa caaaatgcca
    35401 atgcaaacat ggggccccga cctttcagac atcgatttcg atgccatcaa atattaccaa
    35461 aaaccaagtg acaaacccgc tcgtgactgg gaagacgtac cagaaaaaat caaagataca
    35521 tttgaaaaga ttggtatacc agaagctgaa cgagcttatc tagccggtgc ttccgcacaa
    35581 tatgaatcag aagtcgtgta ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc
    35641 tttactgaca ctgactctgc tttgaaagaa taccctgaat tattcaaaga gtacttttca
    35701 aaacttgtac cgccaaccga caacaaatta gcagcgctga attcagccgt atggtcaggt
    35761 gggaccttta tttacgtgcc aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc
    35821 cggatcaatg cggaaaacac ggggcaattc gaacgtacgc tgatcattgt tgacgaagga
    35881 gcaagcatcc actatgtcga aggttgtacg gcacctactt attcaagtaa cagcctgcat
    35941 gccgccatcg tagaaatctt tacaagaaaa gatgcgtatt gccgttacac aacgatccaa
    36001 aactggtcag acaatgtgta caacttagta accaaacgag caaaagcata cgaaggcgca
    36061 accgttgaat ggatcgacgg aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt
    36121 tacttagatg gaaaaggtgc acgaggcacc atgctctcca tcgcctttgc aggagcaaac
    36181 caaatccaag atacgggtgc aaaaatgatc cacaatgcac caaatacatc tagctctatc
    36241 gtctctaaat caatttcaaa agacggcgga gaagtaaact atcgaggaca agtaaccttt
    36301 gccaaaaaca gtgcgggttc gatctctcat atcgaatgtg acacgatcat catggacgaa
    36361 tggtcgaaat ccgatacgat cccattcaat gagatccaca acagccaagt ctctttagaa
    36421 cacgaagcca aagtctcaaa aatctcagaa gaacaactct actatctgat gagccgaggc
    36481 ctgaccgaat ctgaagcaac tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa
    36541 gaacttccaa tggaatatgc agtcgaactc aatcgattga tcagttatga gatggaaggc
    36601 agtgttgggt aaacctgtta taggagctag aacccttgat ata
//
LOCUS       DANKAY010000015        35533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000015 DANKAY010000000
VERSION     DANKAY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            399..899
                     /locus_tag="PWY98_000765"
     CDS             399..899
                     /locus_tag="PWY98_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2036538.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(982..1533)
                     /gene="lepB"
                     /locus_tag="PWY98_000766"
     CDS             complement(982..1533)
                     /gene="lepB"
                     /locus_tag="PWY98_000766"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2036539.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1685..1912)
                     /locus_tag="PWY98_000767"
     CDS             complement(1685..1912)
                     /locus_tag="PWY98_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2036540.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(1909..2427)
                     /gene="msrA"
                     /locus_tag="PWY98_000768"
     CDS             complement(1909..2427)
                     /gene="msrA"
                     /locus_tag="PWY98_000768"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2036541.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2447..3160)
                     /locus_tag="PWY98_000769"
     CDS             complement(2447..3160)
                     /locus_tag="PWY98_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2036542.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3163..4059)
                     /locus_tag="PWY98_000770"
     CDS             complement(3163..4059)
                     /locus_tag="PWY98_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2036543.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4217..5059)
                     /locus_tag="PWY98_000771"
     CDS             complement(4217..5059)
                     /locus_tag="PWY98_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2036544.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5191..5844
                     /locus_tag="PWY98_000772"
     CDS             5191..5844
                     /locus_tag="PWY98_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2036545.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(5907..6434)
                     /locus_tag="PWY98_000773"
     CDS             complement(5907..6434)
                     /locus_tag="PWY98_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2036546.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6454..7401)
                     /locus_tag="PWY98_000774"
     CDS             complement(6454..7401)
                     /locus_tag="PWY98_000774"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2036547.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7413..9299)
                     /locus_tag="PWY98_000775"
     CDS             complement(7413..9299)
                     /locus_tag="PWY98_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2036548.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9459..11537)
                     /locus_tag="PWY98_000776"
     CDS             complement(9459..11537)
                     /locus_tag="PWY98_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2036549.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11550..11825)
                     /locus_tag="PWY98_000777"
     CDS             complement(11550..11825)
                     /locus_tag="PWY98_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036550.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12187..12642)
                     /locus_tag="PWY98_000778"
     CDS             complement(12187..12642)
                     /locus_tag="PWY98_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036551.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12796..14004)
                     /locus_tag="PWY98_000779"
     CDS             complement(12796..14004)
                     /locus_tag="PWY98_000779"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2036552.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14116..14448)
                     /locus_tag="PWY98_000780"
     CDS             complement(14116..14448)
                     /locus_tag="PWY98_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2036553.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14596..15471
                     /locus_tag="PWY98_000781"
     CDS             14596..15471
                     /locus_tag="PWY98_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2036554.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15553..16134)
                     /locus_tag="PWY98_000782"
     CDS             complement(15553..16134)
                     /locus_tag="PWY98_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2036555.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16140..17399)
                     /locus_tag="PWY98_000783"
     CDS             complement(16140..17399)
                     /locus_tag="PWY98_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2036556.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17401..18447)
                     /locus_tag="PWY98_000784"
     CDS             complement(17401..18447)
                     /locus_tag="PWY98_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036557.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18682..18957)
                     /locus_tag="PWY98_000785"
     CDS             complement(18682..18957)
                     /locus_tag="PWY98_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2036558.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19200..20510)
                     /gene="der"
                     /locus_tag="PWY98_000786"
     CDS             complement(19200..20510)
                     /gene="der"
                     /locus_tag="PWY98_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2036559.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20692..21921)
                     /gene="rpsA"
                     /locus_tag="PWY98_000787"
     CDS             complement(20692..21921)
                     /gene="rpsA"
                     /locus_tag="PWY98_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2036560.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22056..22736)
                     /locus_tag="PWY98_000788"
     CDS             complement(22056..22736)
                     /locus_tag="PWY98_000788"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2036561.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22785..23420)
                     /locus_tag="PWY98_000789"
     CDS             complement(22785..23420)
                     /locus_tag="PWY98_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2036562.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23486..24889)
                     /locus_tag="PWY98_000790"
     CDS             complement(23486..24889)
                     /locus_tag="PWY98_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2036563.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(24876..25907)
                     /locus_tag="PWY98_000791"
     CDS             complement(24876..25907)
                     /locus_tag="PWY98_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036564.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            25938..26159
                     /locus_tag="PWY98_000792"
     CDS             25938..26159
                     /locus_tag="PWY98_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2036565.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26324..27061)
                     /locus_tag="PWY98_000793"
     CDS             complement(26324..27061)
                     /locus_tag="PWY98_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2036566.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27214..28554)
                     /locus_tag="PWY98_000794"
     CDS             complement(27214..28554)
                     /locus_tag="PWY98_000794"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2036567.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(28791..30314)
                     /locus_tag="PWY98_000795"
     CDS             complement(28791..30314)
                     /locus_tag="PWY98_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2036568.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30692..31303)
                     /locus_tag="PWY98_000796"
     CDS             complement(30692..31303)
                     /locus_tag="PWY98_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2036569.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31426..31551)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31633..32349)
                     /locus_tag="PWY98_000797"
     CDS             complement(31633..32349)
                     /locus_tag="PWY98_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2036570.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32355..32924)
                     /gene="scpB"
                     /locus_tag="PWY98_000798"
     CDS             complement(32355..32924)
                     /gene="scpB"
                     /locus_tag="PWY98_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2036571.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(32908..33705)
                     /locus_tag="PWY98_000799"
     CDS             complement(32908..33705)
                     /locus_tag="PWY98_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2036572.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33675..34046)
                     /locus_tag="PWY98_000800"
     CDS             complement(33675..34046)
                     /locus_tag="PWY98_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2036573.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34065..34952)
                     /gene="xerD"
                     /locus_tag="PWY98_000801"
     CDS             complement(34065..34952)
                     /gene="xerD"
                     /locus_tag="PWY98_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2036574.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35048..35521)
                     /locus_tag="PWY98_000802"
     CDS             complement(35048..35521)
                     /locus_tag="PWY98_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2036575.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT         9944 a         7474 c         6022 g        12093 t
ORIGIN      
        1 aaagaaaatg gtggtacttt ccacgaagta gatatgggaa tgcctgaaga agaaatgttt
       61 tcattagaag tccaagatct agacggaaat acattagaac ccgtatggat gaagatgtaa
      121 gcagataact aatctgacgt tttttataac aaaaaaacaa aggcggatga tacgataagt
      181 attctccgcc tctatttttt ttattcgtac aatctctttt ttacagttta taaaagcagt
      241 cttctcaccc tttttttaat tgttagatgc cagtgtatgt aaatcaccaa tgtgtcatga
      301 cacattggtg attttatggt atccttttat tattaactga caaaggagag aatattttga
      361 acactttgat tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga ttgttgtcga
      421 cctctgctct tttattagcc cttgatgtca tcttatacaa gatagcgatt ggtcctgctt
      481 attttcaaat cacattcgga tttttaagta tggcgcttat gggttatcta tatggaccga
      541 tctgggctgg tttgcttgaa atgttgtcga acactttaaa tttcacgata tttggttcag
      601 gaacgtttca agctgctttt cttatcacgg ctttccttgg tggatttatt aatggcttat
      661 ttttatatca taaaaagata aacctgcctt ccgtcatcgc tgcccaatta ttgattatga
      721 tcccagtcag cctttttatc aattcttggc tgatgtctgt tttgtttggt gcagatttca
      781 aagtaatttt tctaggtaga ttcatcagga atatgatcca aatccctatt caaataatgg
      841 ttgtttattc atttatcaaa actttagagg ctagaaaagt gtttaaacga gctgtttgat
      901 tttacgtact aaggtagtaa actgattttg gattaaacaa taaagcgtgc agtctcaaca
      961 gactgcacgc tttattgttt ctcactggat ccattttatt tcatcaagtg gataatagac
     1021 aaatcttgct ttacctagaa tatcagtttt attgatcgtg ccaaacgaac gactatcttt
     1081 tgaaactctt cgattatctc caagtacaaa ataattatct tccggaagct tggattgtcc
     1141 cgttagctct tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat ctgatttcag
     1201 gtttttagtt agataaggtt ccttaactac tttcccattg atcttcaatt gatcattttg
     1261 gtagctgaca ttttctcctg gaagaccgat tactcgtttg atataaatcg tgccatctgc
     1321 taattgaaaa acaacaatat caaatcgtct gatctccgaa aatttttcca tcacaaccat
     1381 atcaccttgc ttcaacacgt tttccattga attgccttcc actggtaccg ggatgaataa
     1441 aaatccccgc aacatgaacg caaggaatac agaaacaagt aagtatttga atacaagcca
     1501 gaagcgatta atatagcgtt gtttttttgt caatctctat caatccatcc ctcttttttt
     1561 cattctttct ttattataag aaaaatatca aaaaaatgcg actagatagc tgaaataaaa
     1621 ataattcttt caattaggct attggacaaa aaactttttt gatcataaag tacaaaacta
     1681 tctcttatat gtcatgatta ttttctaaat atttttccca tgcttcgtca aagatatcca
     1741 tggacggcaa atagtcgaca ttcagttcca gataattaga aatttcttcg tagctagagc
     1801 tgtgcttagg aaattggatg tctttaccgg cttctgtagc gaaaacagat aaagcatcat
     1861 gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt
     1921 cctccaattt tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa aattttcagg
     1981 atgcttttta taatactctt gatggtcttc ttctgctaga taaaatggtt gggctggttc
     2041 gatttttgta acgattggct gttcgaaacg tccactttca gctaactgtt tcttactttt
     2101 ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc accgggcgat aattgtcacc
     2161 gcgatcctcg aattgtccga aagcgtcagt tggatcagtt tgctgccagt aaagttctac
     2221 taattgttca tatgaaatca atgttggatc atagataatc tccactgctt ctgtatgtcc
     2281 agtcgtttgg ctttttactt gttcataagt cggatctggt acatgccctc cagtatatcc
     2341 tgacatcact gtatggatac caggaagcgt gtcgaaaggc tggatcatac accagaagca
     2401 gcctcctgca aatattgctc gttccattat gattccccct ctaatactac tcatccgatg
     2461 attttttatt ttcttttgtc ttgctttcag tactgctggt gctagttttg ttggtatttg
     2521 agttgtttga agcatcagag gtatcttttg gcaaatatag actaaaccga atgtcatcat
     2581 ttactaagtc gattttatct gctttaatat acatcccgtt ttgcattcgg aattgatcca
     2641 acctaagcat gatagtcaga tcatctgtat tgacttcgat ccaatctggt aatttatagt
     2701 ttctcttgat catattcatc acatctttga ttggcaaact caatgttccg atagaaagac
     2761 ttctagcttt tagttggaca tttccgtttt ccatcacata cggatcaaaa tataaataga
     2821 aatcgatagg aaaacctagt acttcgaaag taccattgag tagtgcttca ttttccaaat
     2881 aaaatttata ttcaatatca ctatcttttt gatactcact aaggaaaaag ctgatgattt
     2941 gattgacttt ttctttattt gaattgatcg ttaagacagg atctccttgt ctttccgtaa
     3001 gagctggtat ttctgggagt tctggttccc gattagcaaa aatacggctg cctaaaaata
     3061 cggtcgaacc aagtactacc ccaagaagga taaggaaggc gaatttccag taatttgttt
     3121 tctttgtttt gtttgaagat acgtcagatc ttgttttcat gtttattgat catcctcctt
     3181 ctctaaccat tggttttgtg tctcgatcag ctgatctctc actgcatttg ccatcaattg
     3241 ataacctaaa ttatttggat ggaaatgatc ttcttcatag agcacattat ttttgatgcc
     3301 atttagattt tctgtcgtgc tagttgtttg atctgttcct tcttctgtga cacctacttc
     3361 ctgattcaat ccttgataaa gcaaatcatt gattgggata aagtagcagt tcggcatttt
     3421 ttttgtcacc gtttctgttc ctttgttcca attgttaaca attgtctgca tgtcagtgat
     3481 ctcaggaaag ttcaaataaa atggattgta gatgcctaaa atgtaaatag gtgcggtttt
     3541 attgagggat cgaaattctt ccaatagttc tgtgacattt tcctgatagt ttttcagtgg
     3601 ctttttaaag gaattgaccg ttaacccgaa taaattattt tggataactt tcatcaaatc
     3661 attaccgcca acagtcagtg tgataaaatc agctgttttg atattgtttc gaatttcttc
     3721 ttttgttttt actcgcttta agatctggtc acttcgttca ccatttacac catagttttc
     3781 tgtttcaacc gcggtcaaat tataacggtc ttttagatca tttgcgacaa ggggaacgaa
     3841 tcctccttgc ttggtttgat ctcctactcc ttcagtcagc gaatctccga tagctatgta
     3901 gtgaagtttt tcttttttat ttggatcttc tctcacagta gttactggtt tcattagtgg
     3961 gccagctttc ggtatagaag cagctaaacc tgcatagatg atgatcgcaa ttaataatgg
     4021 gaacaaaacg aggatcaaat gtcgtttcgt taatttcatt ttttcacctc ttgtattcga
     4081 ttggttctaa tgttatcaaa aattttatca aaaaggtagt ctttatgaaa aaagaaaaca
     4141 attattttcc taattttata aagaaaaaga gaataagctg caagcctttc aggctgaggg
     4201 cttatcctct tttcgactaa tctacataat acatgattgc aaacgcgtct ttaccagtat
     4261 gcgtagcgat aaccggattc gtatgcagta cagggatatg catgtccggg aataattctt
     4321 gcaaaccctc tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt
     4381 gtcgcacatt tggcaattct ttcagttcat ttttcaaatt attaaaccat ttatcgaacg
     4441 ttttctttcc tcgcccctta gttacaggga ttaattcaga atgggcgaaa ttcatcacca
     4501 cgcgtatgtt caaaacacta gacagcaaac cagttgttcg gctgattctt ccacctttta
     4561 ccaagttatc tagtgtggat attccgatgt ataattttgt attgtcttgt acttttttga
     4621 cttcttcaag gattttctct actgttttac cttcttttgc taattcagct gctcgaatta
     4681 cctgaaaaga taatccttgg tcagtggtat cactgtcgat aaccgttact ttcgtttttg
     4741 ttagattact ggcttgtctt gctgcttcta ctgttccact caagcctttt gtcatatgga
     4801 tcgaaatcac ttcactgccg tcttctccta gctcatcata catcgagatg aattctccaa
     4861 ttggtggttg gcttgttttg ggcagatttt ttgctttcgc catcatttcc ataaatcttt
     4921 cgccgtctag ttggtcatca tcgggatata ctgttccgtc gatcatgatg gatagcggca
     4981 tgacacgaat gcctaaagca tcgcgtacat ttttttccat cgtacatgaa gaatctgtta
     5041 cgatttttac atttgtcatt attttctctc tctctttctt taacaagatg ccttattcat
     5101 gttaaactac atgtaagaca atttctattt ttagtataac agacaaataa tgatttttca
     5161 ttaattacac ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat
     5221 gaaatattaa atgaagtgtt taatgcagtc acccatggaa ttgggactgg attgagtatc
     5281 gcgggactag tggttctttt agtaaaaggt gcccacttgc attcctccct ccatattgtc
     5341 tcttatgcta tctatggatc gatgatgatt cttttatttt taacgtctac tttgtttcat
     5401 tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt
     5461 cttttgattg caggaagtta tactcctttt tgtttgctga gtatccgagg atggctaggt
     5521 tggactttat ttgtattaat ctggttgttt gcgattcttg gcattatata taaagcgttg
     5581 acgttgcata aacgggagag agtatcaaaa gtatctacga tcatctatat ttttatgggg
     5641 tggctctgtg tcgtggctgc tgttcctctt tatcactctc ttggaatgac tggtgttatt
     5701 ttgatggtag caggcgggct ttcttacacg ataggcgcat tcttttatag cttgaattct
     5761 atccgctata tgcatgttgt ttggcatttg tttgttatgt taggtgcagg ttttatgtac
     5821 ttctctgttt tattctttac ttaataaaaa aaataaagct atggatggga cagctctaaa
     5881 taacagctag tctatccata gcttttttat atcgtcaatt cgcctgtccc gctttttaca
     5941 ggattctcat tacgctgata cgtttcaaat tgatgcggat aacgattttt ttcatccatt
     6001 tctccttgac tagtattgat cagtgaccaa tcagaccagt caacaggtgg aaaataagca
     6061 tctccatcga atacatgatg gatgactgta cggtataata tcgtacaaaa tggcagaaat
     6121 tcttgataaa cagctgaccc tccagcgatg aaaaagatac cgtctgtttc tttttccaga
     6181 gcaagcacgt cttccacact atgcatgaca tgcacgcctt ctgctttata ggaagaatct
     6241 ctcgtcaaaa caatcgtctg acgattcggc aacggacggc tacccatccc ttcgaatgtt
     6301 tttcgtccca tcactagtgt atttgcttct gtcatttgtt tgaagaattt cagatcattt
     6361 ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa
     6421 attgcagcta acattttctc atcctttctg attctaaaca gcgataggag ctttgatcgc
     6481 cggatgtggg tcatatcctt ctaattggat atcttccatc tcaaaatcaa atactgactg
     6541 cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt tctcggctca attgttcttt
     6601 catttgttcg atatgattga tataaagatg agcatctcct aatgtgtgaa caaattcgcc
     6661 aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt
     6721 gaacggaacg cctagaaaca catccccact gcgttgatac aattggcagc ttagccgtcc
     6781 ctcttgaaca taaaattgaa acatcgtatg acaaggaggc aaagccatgc taggaacatc
     6841 ttccggattc catgcagaaa cgatcaatct tctagaatca ggagtctttt tgattgcttc
     6901 gatcacttgt ttgatttgat cgataaagcc accgtctttt gtttcccagt gacgccattg
     6961 atagccgtag atgtgtccaa gatcgccgta tgtttcagca aaggaatcat cggttaggat
     7021 cttctcgcaa aaaagcttgc tctgcttttt atattcttca ttgaattcag gatctgtcaa
     7081 acatctatgg ccaaaatcag tcatatctgg tccattatag tctggacttt tgatgtagcg
     7141 ttcaaatgcc cattcatccc aaatatggtt tttattttgt aatagatagc ggatattcgt
     7201 atctcctttt aaaaaccaaa gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt
     7261 tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt tggtatccga ataagcttaa
     7321 tgtgcctgtg cctgttctgt cttctttacg atgcccttct tccaacaatt ttcttcctaa
     7381 gtcaagatac gcttgttcca tcctttttcc tcctaatctt ctacccattc actcaagtat
     7441 tcccaccgtg ccattttttc ttcaagttgt gcttctgttt cagatacgtc gttttgcaat
     7501 tcttgtaatc gagtgaaatt atctccttga tgattcatct cttcttggag caatgagatt
     7561 ttttcttcaa gttctgcaat ctcatcttca atcgtttccc attctttttg ttccatgtaa
     7621 gataatttct tcttttcagc aggttccttg ctctgtattt ttggtttcgc tttttcagca
     7681 ggctgctggg tctctttttg cttcagtaaa taactggaca tgtttccgta ataaagttct
     7741 tgttttccgt ttccttggaa tactaataac ttatctgcta ccttgtccaa gaaataacgg
     7801 tcatgggaaa cagtgatcac agcacctgga aaacttctaa aataatcttc caaaatcgtc
     7861 aatgttgcta tgtccaaatc attggtcggt tcatccaata ataaaacatt aggttgctga
     7921 atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc ctgaaagttt gcctatcagt
     7981 gtcccatgca taaatcttgg aaataaaaat cgttcaagta attctgctac tccgatttga
     8041 ctgccatctg ctgtttttgc ttcttcagca gcttcttgta agtaagcaat catccgttta
     8101 tctggtgcca tttcttcatt ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata
     8161 gacattgtac cgctatccag gggaattctg ccagctagaa tattaagcaa tgtcgatttt
     8221 cccgcaccat ttttaccagt aatacctagt ctttcccgag attgaatcaa taaatctaaa
     8281 tgttctagca gtgtttggtc atttatacgg taactgccat ctttgatttc cagtactttt
     8341 ttccctaaac gttgtgtcgc taggttcagc tctaaatcat cttcttggtt tacttggtac
     8401 aagttctcct ttagatcttc gaaacggttg atccgtgctt gctgcttggt gccacgtgcc
     8461 tgtacgccag ccctcatcca agctaattcc tgtttataaa gccgcttgcg cttttcttct
     8521 tgttctactt ctacacgatc acgttctgct ttttctaaga cgtaagcttc ataatttcct
     8581 ttatactcgt ataattttcc aaatgaaagt tcgaagattc ggtttgctac tctatctaga
     8641 aaataacgat cgtgggtaac catcaatagt gcgccacggt attgtttcaa atagttttcc
     8701 aaccactcga tcgcttcata atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg
     8761 ggttcatcaa tcaacacttg cgccagacta actcgttttt tctgaccacc tgataaagta
     8821 ctgatttttt tatctaattc actgattcct aatttttgca aaataatctt tgcgttggta
     8881 tctgtagtcc aagcatcttc tttattcatt tgttcttctg ctttagcata acgtttttgc
     8941 acatcagggt cttctccgtt ttcccctagt tcgatcaatg cttcctcata tgctcgaaca
     9001 gtttggatca aggggctgtt tccttgaaag actgcttgga gaacagttaa ttcatcggaa
     9061 aattcagctg cttgagaaag ataaccaata cggtaatcag ttggataaaa cactgtttgc
     9121 cgatcaccgt cgctgctatc gataccggca ataatattta agagacttgt tttacccgtc
     9181 ccgttcgtcc caatcaaccc gatacggtct ttttcgtgga ccaaaaaaga aatctggtca
     9241 aacaaatctt tttcaccata agtttttttg aggtcagtta cttttaattc tttcaacatg
     9301 ttccctcctt atcttggata ttgtatcata aaaatagtga acttcaggaa aatttttttg
     9361 gtatttgttc tttttgtgtt ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata
     9421 tgttttgttt tttataaaac ttgaaaattt ttaccttttt attttattgg actcgttttt
     9481 tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt gttcatcgta
     9541 taagattcta acaaattgat attcctgcga tagctgttcg cagaaaacgt cgtgattttt
     9601 tcagcaactt cgtattcatc aatgattttt ctttcaagat aataacctct tagcattttt
     9661 ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa tcaaattccc cgagttcatt
     9721 tttattcctt caagtttgcg ttgctggata aaaaattgaa ccatcgtttc atcataaata
     9781 tcagtcaagt tatctaaact ttcaataatc aactgagtat ttttttgata tgtctctctg
     9841 atttcttgga tatcttcatc actaataaat gtctgacgat cagcatatct tctttgccaa
     9901 aacattttcg gatggagaac gattcgcatt aacctacgga caacaatgaa ccaaaagccg
     9961 ataaaagaca aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa acgggagtaa
    10021 aaacgataac cattagaaga aagcttgccc gaacgataac tttcatccaa tccatcccgt
    10081 tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt gcttttcatc atcggttaaa
    10141 tcttccaagt acagatcacg tatccgttct tgataatttt ctatcactgc ttcagtggct
    10201 aaaaattctt ttgtattacg atcaatttcc tttatttctt ttcgcaaaac ttcaacgacc
    10261 tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt ttccgtcagc
    10321 ataggcaaga caatgatccc tatgaccaaa gtcactaaga tgacacccgc cgtcagaaac
    10381 aaaagcaaag accgctggta aaacatcata ttattgattg aaaatggcaa aataaaaatc
    10441 gtagccaaac tgactgttcc ctttacaccg ccaaatgtta agatcaagat ctcgttcaac
    10501 tgttttttga actttttact gccatctttg aatacataaa aaagactgat agaaatgaat
    10561 ctgatcacaa aaagcatgac agagattaat acgataatcg ctaataaaag accgtttgga
    10621 tataaaccgt ctccccatac tggcgagaac acttgcgtca attcgattcc taaaaatata
    10681 aatactaatg cattcagtgt aaaagcaata gttgtccatg tactgtggga caagctcgac
    10741 aattctgcat caaacacaga gattttccta aaaccagaag cttggagaat acctgctgcc
    10801 actgcagcaa tgattccaga tacatcaaat gcctctgcca atacataggc tagaaaagga
    10861 agcaaaagtt cgatcagcag ataccctgtg acatcttgtg ctgatgcttt ttcgatcaaa
    10921 tgaattattt tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc cccaatacta
    10981 gaaactacta gactcatccc cgcgtttact gctgaaaaag atccggtgat cagcgcacca
    11041 agagcaaact ggaaggcagt aacaccagat gcatcattca aaagaccttc tccttccaaa
    11101 atatgcatcg cctttttagg gatattcact cgtccagaaa gagaactgac tgctaccgca
    11161 tctgtcggac ctaacgctgc tccaaaagca aagcaggcag caagtgggac acttggcaac
    11221 aggaatgata acgtcgcacc taccccgaca agggtcaaaa tgactcctcc aaatgccaag
    11281 aacaaaattg tcccgaaatt tttgagaatc gaagaaatat ctgctttttc tccttctcta
    11341 aataacaatg gtgcaataat cataactaag aacaattcag gttcaaaatc gattgattgt
    11401 ccccactctg tcagacctaa taccactcca aggaaaattt ggatcagtgg agctggaatc
    11461 attggcaaaa tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa tacaataact
    11521 aactcagcaa attccatcgt ccatcccctt tattttttat tttgtgtaag catttttaaa
    11581 aattgttctt ttttctttaa aattttggtt tcgactactt caacctgtgt ttggttcccc
    11641 tctaattcac actgtacacg ttttgactcc aaacgaagca gatcatgttt cagacgttcc
    11701 aactgacgat acatcaatat atttctttta cgcaacttac aaagtatttg acggcggtca
    11761 tccatcgaaa gggcgccata gcgatcatgc aacagttttt tttgttgttc aaaactctct
    11821 ctcattttcc agatctccta tacacaaact tccatcatta tagcaaagtt ctttttggat
    11881 gaaaaatatt tcattgtttc atcgtttatt gctagttaga aatttccata aatttttcaa
    11941 cgcctcattc tattccaaat agtatcagat atcaagtaaa acttaagaaa gataaaagtt
    12001 tctgacaaaa ggatgtttac tttcccattc ctgcctttct tgttctttta tcacaacaaa
    12061 aaaatggggc tgcgacaaaa atcttgtcac agtcccttat tctgaataaa cggtgtggaa
    12121 gagacagcct atcagtagta ataagacaca tctctttcac agcctcattt ttgcgtaaaa
    12181 atcgatttat tcgaaaaatt ccccgtatac atcttctaca cgttgatctg gattgatcac
    12241 gatatacaag ttacctgttt tttcgctgat ttttgttgat tgatattgat tgtctaatcc
    12301 ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat ttcggtaaag
    12361 gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc ccatttccag
    12421 tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt
    12481 aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt
    12541 tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc
    12601 atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga ctcctttatg
    12661 atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg gtcacatctt
    12721 atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag ctgagcagat
    12781 acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa cctgtttttt
    12841 atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag cttcactaac
    12901 ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta ctgctaattc
    12961 gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca tctgaggtgt
    13021 ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc ctaagaagta
    13081 caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga caagtgcttg
    13141 aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac gaatctcatt
    13201 ttcttttaga tccagttggc tgatcaataa tgcccatgct tggctctctt ctttcaatgg
    13261 gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca aaccgggaca
    13321 gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt catttcccaa
    13381 gagcatcttg atgaattcca catttatacg ttctacagat attttaccta atagctcgtg
    13441 gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt gactgacaaa
    13501 acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag gattgcctac
    13561 agctcgcaag atctgttctt ctagatcttg cagaccatcg aacaaatcga tgatttctcc
    13621 atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat cttctgccaa
    13681 cgaacgaacg aattcgacat gatctggtct acggtagtcc tgataagttg attccgtccg
    13741 aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc catgttcgat
    13801 cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg cgcttgttgc
    13861 gatatccaca tcatggattg gctggttcag caagatatca cgaacacttc cacctacaaa
    13921 ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct tttggtattc
    13981 cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct tgtcctgaga
    14041 taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt caaggccagg
    14101 accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc tttgattgaa
    14161 tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat tagccatgat
    14221 catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg gttcagaagc
    14281 ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac ctacttcttc
    14341 agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt gctggatata
    14401 ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct ttttccctcc
    14461 aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa
    14521 tttccatgct acatttagga aggaatttct aaatggaaat tctgtgaaaa tacataattg
    14581 aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt tatcattata
    14641 ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca tttagcagaa
    14701 gggggagtct cagggatcac cttgatctta agagctttat ttggtattga tccagcttat
    14761 acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct cggtaaacgt
    14821 tccttggcct atactgttct aggtacactt tctttgtcat tctttttatg gatctggcaa
    14881 agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct acttgcgggt
    14941 ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac aggcggcagt
    15001 gatattatcg ctcggatttt agaacaaaat tacggcatca ctatggggcg ttccctattg
    15061 gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa acgtatgatg
    15121 tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt agatggcgga
    15181 tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc accactactg
    15241 atgaccggtc tgcaaagagg tgtgactttc ctttctggag aaggcgcatt ctctggtaat
    15301 tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc taagaggatc
    15361 gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga agtggaaggg
    15421 caaggcttta cttatttgaa gccacagaca agattactga aaaaactata agaaaaaaga
    15481 caaagatagg ctatgaggaa agattccaca tccagctgac ctttgtcttt tttagtacta
    15541 tactattttt aactaattgt tatgttcact tacccggtta cttttctttt gtgtctgcaa
    15601 aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat ctccatcttc
    15661 tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt ctttttcttg
    15721 aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc aagaagaaaa
    15781 tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt cgaatataca
    15841 ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct ggtctgtgtc
    15901 cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag atagatcact
    15961 gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat tgcttaaaat
    16021 cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc taaaagatac
    16081 atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta gcatttttct
    16141 cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg ttcaagtgtt
    16201 aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct taagaaaaag
    16261 gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg ttcatagtgt
    16321 acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt ccattctgca
    16381 taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc atatctttca
    16441 tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc tcctacttct
    16501 aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa agcattttcc
    16561 gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc ttctaacact
    16621 gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata tagatataac
    16681 gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc ctgctgattt
    16741 tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt cctttcttct
    16801 ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat acttaaggat
    16861 tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct tgccaacaat
    16921 gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc
    16981 gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa ttttgcttca
    17041 ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa gctttgcgga
    17101 tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc gatctcatta
    17161 tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg gtattggttg
    17221 atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag caattcttcg
    17281 cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa aaagatttgt
    17341 gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt tgtattcatt
    17401 tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc tcaataattc
    17461 tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa caagtatttt
    17521 tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg aaatctgttc
    17581 cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt ttagttggaa
    17641 atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta aaaatttttt
    17701 catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt caaacagttt
    17761 accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt cttcgatcat
    17821 cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat acagattttg
    17881 caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt caatctgctt
    17941 tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg gcagattgat
    18001 ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg
    18061 gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg ttgtatgttt
    18121 ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga taaacatgga
    18181 ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg ttaaatgatt
    18241 gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa ataacgggtt
    18301 gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga ttggctgatc
    18361 aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt tcgtcaacgc
    18421 atcttctact tgataatcaa gttccatacg catccctcct cttcttattt ttattttacc
    18481 attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact tgcaaataaa
    18541 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
    18601 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
    18661 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
    18721 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
    18781 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
    18841 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
    18901 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
    18961 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
    19021 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
    19081 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
    19141 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
    19201 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
    19261 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
    19321 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
    19381 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
    19441 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
    19501 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
    19561 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
    19621 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
    19681 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
    19741 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
    19801 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
    19861 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
    19921 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
    19981 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
    20041 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
    20101 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
    20161 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
    20221 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
    20281 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
    20341 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
    20401 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
    20461 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
    20521 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
    20581 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
    20641 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
    20701 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
    20761 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
    20821 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
    20881 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
    20941 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
    21001 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
    21061 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
    21121 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
    21181 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
    21241 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
    21301 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
    21361 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
    21421 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
    21481 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
    21541 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
    21601 aactttttta gcatctaaac ggcgttttga aagtagatag ctgccgtttt ctttatcttt
    21661 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
    21721 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
    21781 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
    21841 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
    21901 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
    21961 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
    22021 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
    22081 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
    22141 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
    22201 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
    22261 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
    22321 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
    22381 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
    22441 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
    22501 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
    22561 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
    22621 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
    22681 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
    22741 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
    22801 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
    22861 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
    22921 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
    22981 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
    23041 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
    23101 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
    23161 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
    23221 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
    23281 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
    23341 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
    23401 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
    23461 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
    23521 aaatcgttcc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
    23581 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
    23641 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
    23701 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
    23761 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
    23821 ctgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
    23881 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
    23941 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
    24001 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
    24061 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
    24121 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
    24181 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
    24241 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
    24301 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
    24361 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
    24421 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
    24481 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
    24541 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
    24601 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
    24661 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
    24721 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
    24781 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
    24841 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
    24901 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
    24961 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
    25021 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
    25081 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
    25141 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
    25201 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
    25261 aaaaggtgga tacatctgct ttgttgcaga taaaccgctg actcatcatt gttttttgcc
    25321 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
    25381 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
    25441 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
    25501 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
    25561 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
    25621 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
    25681 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
    25741 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
    25801 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
    25861 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
    25921 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
    25981 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
    26041 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
    26101 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
    26161 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
    26221 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
    26281 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
    26341 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
    26401 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
    26461 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
    26521 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
    26581 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
    26641 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
    26701 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
    26761 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
    26821 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
    26881 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
    26941 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
    27001 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
    27061 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
    27121 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
    27181 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
    27241 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
    27301 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
    27361 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
    27421 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
    27481 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
    27541 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
    27601 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
    27661 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
    27721 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
    27781 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
    27841 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
    27901 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
    27961 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
    28021 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
    28081 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
    28141 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
    28201 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
    28261 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
    28321 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
    28381 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
    28441 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
    28501 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    28561 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    28621 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    28681 agaaaaaaca ctcactgaat ggagctattg acttatccac gtgcttatcg atagccgttt
    28741 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    28801 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    28861 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    28921 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    28981 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    29041 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    29101 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    29161 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    29221 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    29281 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    29341 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    29401 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    29461 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    29521 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    29581 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    29641 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    29701 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    29761 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    29821 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    29881 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    29941 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    30001 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    30061 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    30121 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    30181 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    30241 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    30301 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    30361 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    30421 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    30481 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    30541 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    30601 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    30661 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    30721 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    30781 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    30841 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    30901 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    30961 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    31021 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    31081 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    31141 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    31201 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    31261 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    31321 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    31381 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    31441 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    31501 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    31561 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    31621 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    31681 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    31741 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    31801 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    31861 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    31921 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    31981 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    32041 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    32101 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    32161 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    32221 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    32281 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    32341 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    32401 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    32461 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    32521 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    32581 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    32641 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    32701 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    32761 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    32821 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    32881 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    32941 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    33001 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    33061 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    33121 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    33181 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    33241 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    33301 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    33361 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    33421 gtctgtgacg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    33481 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    33541 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    33601 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    33661 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    33721 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    33781 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    33841 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    33901 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    33961 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    34021 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    34081 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    34141 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    34201 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    34261 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    34321 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    34381 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    34441 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    34501 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    34561 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    34621 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    34681 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    34741 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    34801 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    34861 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    34921 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    34981 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    35041 tagacactca gacggactgg tttctttttt ctttttcata gcattcctga cagattccat
    35101 ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact acttgctcca
    35161 cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag catagtaaat
    35221 gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat ccatcattaa
    35281 aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga taaacagtag
    35341 caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct gcagataaat
    35401 gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt gtcagcttaa
    35461 accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca ttcatgacca
    35521 tttactcaac ccc
//
LOCUS       DANKAY010000016        35203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000016 DANKAY010000000
VERSION     DANKAY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            92..892
                     /locus_tag="PWY98_000803"
     CDS             92..892
                     /locus_tag="PWY98_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036576.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            980..1786
                     /locus_tag="PWY98_000804"
     CDS             980..1786
                     /locus_tag="PWY98_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036577.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            2018..2887
                     /locus_tag="PWY98_000805"
     CDS             2018..2887
                     /locus_tag="PWY98_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2036578.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            2907..3254
                     /locus_tag="PWY98_000806"
     CDS             2907..3254
                     /locus_tag="PWY98_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036579.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            3267..4529
                     /locus_tag="PWY98_000807"
     CDS             3267..4529
                     /locus_tag="PWY98_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036580.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            4609..5985
                     /locus_tag="PWY98_000808"
     CDS             4609..5985
                     /locus_tag="PWY98_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2036581.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            5982..6611
                     /locus_tag="PWY98_000809"
     CDS             5982..6611
                     /locus_tag="PWY98_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036582.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            6623..7093
                     /locus_tag="PWY98_000810"
     CDS             6623..7093
                     /locus_tag="PWY98_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036583.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            7090..7572
                     /locus_tag="PWY98_000811"
     CDS             7090..7572
                     /locus_tag="PWY98_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036584.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            7569..8231
                     /locus_tag="PWY98_000812"
     CDS             7569..8231
                     /locus_tag="PWY98_000812"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2036585.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            8218..8586
                     /locus_tag="PWY98_000813"
     CDS             8218..8586
                     /locus_tag="PWY98_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL2036586.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            8667..9113
                     /locus_tag="PWY98_000814"
     CDS             8667..9113
                     /locus_tag="PWY98_000814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036587.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            9765..9818
                     /locus_tag="PWY98_000815"
                     /pseudo
     CDS             9765..9818
                     /locus_tag="PWY98_000815"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9822..10421
                     /locus_tag="PWY98_000816"
     CDS             9822..10421
                     /locus_tag="PWY98_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2036588.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            10437..10913
                     /locus_tag="PWY98_000817"
     CDS             10437..10913
                     /locus_tag="PWY98_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036589.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            10930..11832
                     /locus_tag="PWY98_000818"
     CDS             10930..11832
                     /locus_tag="PWY98_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036590.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            11905..12096
                     /locus_tag="PWY98_000819"
     CDS             11905..12096
                     /locus_tag="PWY98_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036591.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            12260..12847
                     /locus_tag="PWY98_000820"
     CDS             12260..12847
                     /locus_tag="PWY98_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036592.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            12847..13746
                     /locus_tag="PWY98_000821"
     CDS             12847..13746
                     /locus_tag="PWY98_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL2036593.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            13746..14660
                     /locus_tag="PWY98_000822"
     CDS             13746..14660
                     /locus_tag="PWY98_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036594.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            14712..14927
                     /locus_tag="PWY98_000823"
     CDS             14712..14927
                     /locus_tag="PWY98_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036595.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            15112..15366
                     /locus_tag="PWY98_000824"
     CDS             15112..15366
                     /locus_tag="PWY98_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036596.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            15545..16588
                     /locus_tag="PWY98_000825"
     CDS             15545..16588
                     /locus_tag="PWY98_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2036597.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            16761..16985
                     /locus_tag="PWY98_000826"
     CDS             16761..16985
                     /locus_tag="PWY98_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036598.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            16988..17245
                     /locus_tag="PWY98_000827"
     CDS             16988..17245
                     /locus_tag="PWY98_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036599.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            17347..17700
                     /locus_tag="PWY98_000828"
     CDS             17347..17700
                     /locus_tag="PWY98_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036600.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            17753..18547
                     /locus_tag="PWY98_000829"
     CDS             17753..18547
                     /locus_tag="PWY98_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036601.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            18576..19694
                     /locus_tag="PWY98_000830"
     CDS             18576..19694
                     /locus_tag="PWY98_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036602.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            19727..20047
                     /locus_tag="PWY98_000831"
     CDS             19727..20047
                     /locus_tag="PWY98_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036603.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            20302..20991
                     /locus_tag="PWY98_000832"
     CDS             20302..20991
                     /locus_tag="PWY98_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDL2036604.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            20992..21195
                     /locus_tag="PWY98_000833"
     CDS             20992..21195
                     /locus_tag="PWY98_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036605.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            21215..22843
                     /locus_tag="PWY98_000834"
     CDS             21215..22843
                     /locus_tag="PWY98_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036606.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            22894..23265
                     /locus_tag="PWY98_000835"
     CDS             22894..23265
                     /locus_tag="PWY98_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036607.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            23350..23550
                     /locus_tag="PWY98_000836"
     CDS             23350..23550
                     /locus_tag="PWY98_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036608.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            23684..24910
                     /locus_tag="PWY98_000837"
     CDS             23684..24910
                     /locus_tag="PWY98_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036609.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            25507..25902
                     /locus_tag="PWY98_000838"
                     /pseudo
     CDS             25507..25902
                     /locus_tag="PWY98_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            25980..27019
                     /locus_tag="PWY98_000839"
                     /pseudo
     CDS             25980..27019
                     /locus_tag="PWY98_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            27032..27970
                     /locus_tag="PWY98_000840"
                     /pseudo
     CDS             27032..27970
                     /locus_tag="PWY98_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            27963..28310
                     /locus_tag="PWY98_000841"
     CDS             27963..28310
                     /locus_tag="PWY98_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036610.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            28625..29482
                     /locus_tag="PWY98_000842"
     CDS             28625..29482
                     /locus_tag="PWY98_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2036611.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            29502..30062
                     /locus_tag="PWY98_000843"
     CDS             29502..30062
                     /locus_tag="PWY98_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDL2036612.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            30951..31718
                     /locus_tag="PWY98_000844"
     CDS             30951..31718
                     /locus_tag="PWY98_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDL2036613.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            31744..32289
                     /locus_tag="PWY98_000845"
     CDS             31744..32289
                     /locus_tag="PWY98_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036614.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            32290..32856
                     /locus_tag="PWY98_000846"
     CDS             32290..32856
                     /locus_tag="PWY98_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036615.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            32871..33119
                     /locus_tag="PWY98_000847"
     CDS             32871..33119
                     /locus_tag="PWY98_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036616.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            33138..33377
                     /locus_tag="PWY98_000848"
     CDS             33138..33377
                     /locus_tag="PWY98_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036617.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            33394..33978
                     /locus_tag="PWY98_000849"
     CDS             33394..33978
                     /locus_tag="PWY98_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036618.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            34085..34366
                     /locus_tag="PWY98_000850"
     CDS             34085..34366
                     /locus_tag="PWY98_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2036619.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            34356..34697
                     /locus_tag="PWY98_000851"
     CDS             34356..34697
                     /locus_tag="PWY98_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2036620.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT        13398 a         4599 c         7201 g        10005 t
ORIGIN      
        1 ggaaaagcta taaagaaata gataagatgt agaaaatctt atttgtgaat gaaaaacaag
       61 tcatcgtaaa atattaatag gaggtgtagt aatggtggga atatcaaaaa cagctttaga
      121 ctcattagct aaactattta gtagcaggtt tgatagtgca gaatttgttg aatatctaag
      181 ccaaaacgaa agtggaaaaa ctctgatcga aacaatgaaa aaagactatt tgaatgaaaa
      241 tgactttccg acatggacga atattgaaga tgctacatgg gatgtagtgt gtacgatata
      301 ttctccttgg gtaacgattg ctgattttgt tttagataaa attggagggg atacataccc
      361 tgatgccaaa tctcttgcta caagtgactt taaaacgttc tatggttatc taataggtct
      421 aataggcaat ggaatagatt tgaaagttac gccaaaacaa acacctgaag aaattgaaaa
      481 agaaaaagga gaggttcaaa tggcttatgc aaatgacaga gaaaaagtaa aattattgga
      541 aggtaaatta tctgaagaag aacttggaaa aatggtgcaa gaaatcaatg aagacagcaa
      601 ctattttcct gaattgaagg tatatcaaaa tgacgaagat ttctttaatg aaatgtttag
      661 taatagtcca tgggaagcag tgagtaaaac aaatttagga gaatggaact ttcaagatga
      721 tctagttgca tttagcaatg atgggtattt acgatcatat tcagaaagtg agtatagagc
      781 tgaattaaaa acgtatgaaa cggatattct gatgagctat ttaaatctat tagagagtgg
      841 tcagggagag ttaagagata aagttgaaga actattaaaa gaagagctat agggattatt
      901 tcaacataaa cacaagcaga aaactaccaa acaaaacgta ccatgcaaaa agtcggttaa
      961 accggctttt tgctctatta tgagaggaga tcaaaccatg agtgcaacaa ccgatatggc
     1021 tttagaagca gtaataaaag ataatacagg aaatgtcgaa aatgtagaag actttagcga
     1081 tattgcaatc aatctttcag catggatttc agctgtagga gaagaaaata tcgaatttga
     1141 gatcaagaat atggatgact taaaagcaaa atttcctttt atcgcttcga aactaagtca
     1201 atttgccgag gataaatcgt ggaatgaatt agttagtttt tccgaaaaaa tttatgatat
     1261 tacgcaagaa gatgagtgtg tttcatggga aatgaataat caatacaaga aaattgatgc
     1321 tttcgtcaaa tcacgtatag aaggaacagc ttttgagttt gaaggtatag ataccgcaga
     1381 taattggtta aataaagaat cctttattaa aagtatagcg gatgatcttg aaaaacaatg
     1441 gtctaaggaa gactatgcag agtacctaaa aacagaaaga gatacagagg tgaaagaaaa
     1501 actcactgag gtatgtttgg atgaacatat tgatcccaat atcgtaaaac aagaagacta
     1561 tgaagtggtt attgataaag ttcctactga ttttgaggaa gttgtaaagt atatagttga
     1621 agaagcatta tcgcaatact cttcagatga ggaagtggac gaagaaactg ttcgtaccag
     1681 agtttggcaa ctggtttttg atgacaaaga ttataaatat ttgagtgatc aaaactcggt
     1741 taattggagt gtacgattat taattgatca aaatgatatt gaataaaata gagctgaaaa
     1801 gctctatttt ttttgataaa aaaactagct atttttttaa aaataatttg gttcaaaatt
     1861 tattttttta acttgataat tttttgccat ccttttttaa attggctata tatcaaggtt
     1921 tatagtgggg ataaataaaa atacatttga caaaatttac aatatagttt attatatatt
     1981 tatcttatta tgataacaca ataaaaggaa tgatggtatg aaaaagaaga agtttagtcg
     2041 atcgttgtta ggaattttag ttttagttgc ggttggtctt agtggcaagg gttactttca
     2101 gaataacagc actcaggaac atgattttca gatcaaaagt agtgttcagg taggtaataa
     2161 agcagacagt gaatcaaaag aatttttagg gatacgttat gatccaaaca aatatccaaa
     2221 taatatcgta aacattaatg atgggaaata tacactgaca aaagaagacc atgaagcatt
     2281 atcagaaaaa ggcagtcaaa ccttttgggt aaattatgca gatttagatc aacttggaag
     2341 agcaggagat gttgaagcgt tgatcaccta tgatgctata aattctcact catctaaaaa
     2401 tgtggaacgt ccatctttca aaagttctgt ccatattgca ggagaataca aggatggagt
     2461 atttgatcaa gagagagata cttgggtcgg caaagattca aacaatgaaa tcactcagct
     2521 agaaggatat aaaggttatc tctataacaa aagtcattca ttagcatggt cgctaggagg
     2581 agatatggag gtacacaatt taacacttgg aacgagagcg caaaatgttg ggaaaaatga
     2641 tcagaatgga gggatggctt actcagaaac gattgtacgt gatgcaatct ataaaaataa
     2701 gaatgaaaaa gtattgtata aagttgatcc aatttataaa gggaatgaat tggttcctcg
     2761 tggttctcat gtcgaagctt tctcagttaa cgataatgga aaaacggtaa accttaatgt
     2821 ttatgtattt aacaatcaaa aaggaatcaa catcaactat caaaatggta cttgggtaag
     2881 agattagaaa ttgagaaggt gaagaattgg aaacaataag catcaatgga ttagataaat
     2941 cagttgcaaa tcgaataaaa aaaggaaatg gctactatct tctgaagaag ggtaaatatt
     3001 cagttgctag agagttagaa acaatgagta tcgtgtatgt agctaagaat cattttgacg
     3061 tagaacaggc tatttttgat ccagattctg aaatagggaa atgtgttgaa ttatcagaga
     3121 aaactgttag tacaaaaggg gcgatgatca ttgagagaga aatgttagac gaaatcagag
     3181 taatcattcc taaagatacg cagatgtgtg aggacagcat aagtgacgat gaaccaaaat
     3241 cagaaagaaa ataaggagat attcagatga gcgaaaaatc cattgaatta aaggaatata
     3301 agcgaagcga tggagaaaaa tattatcctg tagccagttt ttctaaatca gtatatctgg
     3361 ttggacgtgc attggtagtt ggtgcaattg gcatatatat tttgcattgg gtaacaaagg
     3421 ttgtacaact gcttgtagat accttagtaa gtattcttgt ctcgttaaat gatgttaacg
     3481 gaacagatga tttaggatta aaagaaatgt ttaaagagtt actcaatgcg ttttttccca
     3541 attatttaag ttattcatat aatgtacaaa gcttttttgc aaagctacac ttggtagaca
     3601 tctctatttt agtattagaa gcaggaacgg tattctttgt tggatatttg gttactttga
     3661 tgttcaggca tcactatgga gaacaaagac cattttttga tgatatggaa agtagaaggt
     3721 tgaaaagaga agctctagaa gctatggatg tgaagtatga tccaaacaag attataggag
     3781 atgaaacgaa caacaaaaaa agaaaacgaa acaaaacgca attagaatcc gatgtgctat
     3841 caacgcttaa aaatatcaaa atttctgttc atacaagacg tgatctaagt gtcaatgaag
     3901 atattcgaga atatatcgta aaaatcccgc aaccaaaagg gaagaaagtt agagaaacag
     3961 ttttacgaca aattcaagat ttagggtcga tcctgacaag agcaacaagc ggacgagcca
     4021 aatttggtga aaaagaagat gaagtttcag gcgattattt cattttccga gcatcagtaa
     4081 atattgaaag agaacgtaag gaaagagtaa gtaccaaaaa caaagaaacg gaaagagcaa
     4141 catcttcaat cgcagtttca aattcacaat tttcttttcc aatcaatctc tttgccgata
     4201 attccaaaaa tatccaaaac caaaccgaaa aagctagaaa atatgccgaa actcaaaagc
     4261 aatctttaga actttatcta tcttctgctg acgtacctgc aagtctaaca gatatgcacg
     4321 ttgggaatac atctattgaa tatttgttta ctttatctcg taccagaaaa attcaactaa
     4381 gctcaatgaa agaagaaatt gaaacttact tgcatatatc aggtgtaatc gtgcggatca
     4441 acgcagggcg tattcaaatt actctaccat taggagaaaa tgatcgtatt ccaattgatg
     4501 taccatcaat gatcaatagc gtgttttaaa agacatcaca aatagaaaca gaaaatacaa
     4561 actaccagtt cacaaacaga aaagtaccga gaaaagtgag gaatagaaat ggctatgaaa
     4621 ttattagaga aagtcaaaaa gagagccgaa aaagctgaat ctattttgct agataaagaa
     4681 cctaatataa agcttaatgg aaaattcagt gtcaatatgt tgaacacaaa ggctaaagac
     4741 ccgacagatg ctatttttgg aattaagcct gatggcacaa ttgctcattt ttgcatcagc
     4801 gaaatgccac acatgttggt tgcaggaacg acaggatcag ggaaatcagt attactgaat
     4861 gaaattcttg ttacagcaat gtgtcactca acgcctgatg aattaaaaat aggaattgta
     4921 gacccaaaaa gggttgaatt tggaagatac aaaaaattgc cgttcatgtt ggctgatcca
     4981 attacagata tggatgaagc ttatgatttt tttgaatatc ttgtgatctt gatgcatgaa
     5041 cgttacaaac tgatggaaaa agcagggtta caaaacatca cgctatataa cgaatatgcc
     5101 gaaaaaaatg gattagatag atttccctat gtgatcttat tagttgatga atattcacag
     5161 ttggttggaa cacataaaga agtagaaggt ttgattgttc aattaggaca aatggcaagg
     5221 gcagcaggta ttcatgttat cttagcaaca caatctcctc gatcaacagt tgtcactggt
     5281 attatcaaag ccaattttcc gtcacgtgtt tgccttatgg tagcaagtga tttagaaagt
     5341 aggattgtat tagatgaagg cggtggagaa agtctaagtc caaagggaga tatgcttatt
     5401 aagcttgttg atggaagtat ggttagagcg cagggagctt acatcagtaa caaagaaatt
     5461 gaaacaatct tcaatcacct tagaaatact atgccagaac cagaatatgt tgattaccga
     5521 gcaatcgtag ctgaaactcg tggtgagggc ggtgaagacg aggattttgg tggaattggt
     5581 aaagctcctg taagaaaatc tgctaaaaga aatttgaaca cacctttagc aaatccatca
     5641 tctaaagagc catcaattga aaagaagagt gcgtttgcta ttaagcttga aaaaaataaa
     5701 gaagaaaaag ctaatcaggg agctaagtca ctagaaaaac ttcagcaaga aaaattagaa
     5761 aaactagaaa aagcattatc atcatcaact tctgaaactc aatcttcaaa gggtatagat
     5821 aatggacaat caattacaac taaaaacaag cctaaaaata gtaattcaaa tctctcggta
     5881 aatgttgtta tcacaactga taaatctgaa aaaaaacaag tgaacggaac tacttcaccc
     5941 atcatgcaaa aaatgataaa ggcaaaaaaa caggtgcaga aatgattatc attagttcct
     6001 ttttgagatg gctaacacca tataaatata cagatgaaat agttgagaag cgagcgaaga
     6061 aaaaagttaa aaaaacgatg ttaactccta aagaaatgct agatcgtgga atattgggag
     6121 tgaaggtttt agaagtaaac caaccgtttg aattggataa tgcgagagat aggttgctta
     6181 gagctgaagt tgatgtcgca tacttttatc atgataaaag caagcatcaa atagctatca
     6241 aattcaatcc aaaatcggca tggcgatacc aagcaagaga tgacctaaag cgtaggaagc
     6301 tgaaaccgat gctctatcct gaaggaaaag aggcatttga tagaacccat attgttccca
     6361 ttggatttca tggtacagag tcagataacc gcctattggt agggtggaac agctcacaaa
     6421 atagagttca gatcaataaa tttgagaaga aggtaagaaa atttaacgat cggaaaataa
     6481 tactttggtt tgccaatatt cagaagcaac cagatgggac agcaaagtgg gaagcggtca
     6541 tatttgatga agatggcaaa aaagtaatgt ctgaagtatg gcatgataaa gctaaatttg
     6601 tttggcagta gaggtgaaaa ttgtgttaac taacctaaaa ctatatagag agggatttaa
     6661 cgaaaaagta agtatcggtg aatctattga atatttagga aagaagtaca ttattattcg
     6721 tattcttgaa attaaaaaac tttattttag taaatataat aatttagcag ttagagttct
     6781 cgtccaaaag gttggaaatc caagtgacta tagaagtgat taccataact ataaaacaga
     6841 atcatctgta attaagcgtt ataatcaatc aaaaaatgat gagagaatat tcaaagtagg
     6901 agaaattatt atttctaagt atgggatagc atacgaaata gtttctatta aaaaatttta
     6961 ttatgaattt gttgatttgg ttgtagaata ttctgttcga ttgatcgttc cgtggagtaa
     7021 agaagaaatt aatgaggcat taaaacatga acgaaaaagt acattcaaag ttttagaagg
     7081 tgaaaaaaag tgaaaacaga ccttactagg caagctgaga agtgcttatg gcatgaaaca
     7141 aatcgcatgg gtgtatttgg ttgtttcgaa gtcactatcg gttgggatgg cagtgaaaga
     7201 gtagattata taacttattc agtagataac acagttcgat gttatgagat caaagttact
     7261 ttagctgatt taaaaagcaa ggcgaaacag acttttatcg gtgattataa ctatttagtt
     7321 gttactagtg atttgtggga gaaaatgcaa caagacgata aacttatgcg gaaatattac
     7381 tatcaaggcg tatacgtgtt caatgaaaaa tatcctagca taataagtgt taagaaagca
     7441 aggaaacaaa aagtatcgct aggtacaaga tcaactgtat tagaaagcat ggttcgttct
     7501 atgaatcgtg aagttaagaa actctataaa gtaaaaccat tttgggagat agcagaggtg
     7561 gaaaaaggat gattcaaaca tttgcaaaaa tgagtagaag tgcttggatt gaaaagctgg
     7621 aaaatgattt gagtgttatg cgattagctc caaagaaatt caaacgagag attgcagaaa
     7681 aagaagcaat tttgtcggta ttgaatgcgg tagaactgga cgaaccgaaa aagtcagttg
     7741 atgtacctga aaatgtagca gagttactag attattatcg taattcgtca gatgtcgatt
     7801 tgttagctct gatattaaca tttcacgatt ggtattactg tcaaaatgaa gatactcagc
     7861 atgataaaca tgataaagca gttgactggt taatagagaa tccagaaaag ttcatgcgtg
     7921 cttggctaga tggctatgat agcaagaaaa agccactgga aagtaatgag gaaacgtatt
     7981 taacggcaga acaatatgac tatgctagaa aaacaggaga ttgggaatct ggtaagactt
     8041 tcaatcacag aaaagatcaa ccaaaaatta gtttttcaga tatagccgca attatcgaag
     8101 gtatctacta tggacaagcc gatgaagaga tcttttctat acaagattta ttagaatttt
     8161 tgaacaaaaa taaggatagc tttgtagaac taactgatta tgaggaggat acagcaaatg
     8221 gaaaattatg atccaaatat gcgatggggt acacatacgc ttaaagtttc ttttcaacgt
     8281 tgggattaca agggatttgt gacttttaga aaagctggaa attgtaaagg attagatgtc
     8341 ttagcgttgg atgaagacta tctatatgac catccattga cggataatcc aatcggattt
     8401 ggattgcttc ctgaagatga tgagggcaat gaatggttca aaatgattct tacaaacgat
     8461 aaaggtgatc aattatttgt agaagatact tggtcatatt tgagtgaata tattgttagc
     8521 attaaaatca ttgattttgt agcagataaa gaaaaagaga ttggagaagg taaatctaat
     8581 tactagctaa tgaatagcaa ctttggagga taagtcaaaa gaataaaaaa agaatactgt
     8641 gtgtcattgc agggatgtat ctttgcatga tagtatctgc ggttaccatt tattggttag
     8701 gaaatcagca aaacagttta attgttgatc tgggggagtt aaaggttgga ttacgaatga
     8761 ctagatggct tttattagct caaacagttg gtttagccat attgacttgg atatgtgtgg
     8821 aaaaaataac atctaataat aatcaagaga ttgttgaatc aacaaaccta agaataactg
     8881 aacaagcaat tgaaaagcaa aaagacaaag aaaaaaagga aataatgtca atttatttat
     8941 ctagtggagc atcatttttt catgaaatag attcgatgaa actgagtagt caagaaatca
     9001 ttgatagatg gaaaaacatg cctctaggtg gtgagttaat cgttgaagat agtgatggtt
     9061 ggtcagctta taagaaggac gagattgtag ctatcacgat tttgtttgaa tagtaatata
     9121 ttaccactag ataatctgaa tatgttgatc tgctagtgac gtaccaaatg cgtttaatag
     9181 aaccttggtc acaggcagaa attgataaag cagtaaaatt gaaccgatta tcaaaattta
     9241 acgtattagg aaatgccttt taaaaatcgc aatacacaaa cagaaaaata ccaaaaaatt
     9301 agcaatgaac gtaggtaaga acatgacaat aaataataat aaccctaaca aatataatgc
     9361 tcttgtccaa tgctatatcg aaaacaaaat tacaatgcaa gaagtcgctg atgaattagg
     9421 caaatcaaaa agcctagtat cacacgactt taagcacaat gtttttccga acccgattga
     9481 gctgaggaaa aaatttcaaa agagagttta tcaagaaata gggaagtgga ttgatgaagg
     9541 gaatgcaatt gaccctttaa ttgaaaaata tcctttttta aaaagcaaaa ataaaattca
     9601 aaggttaatt cgtcaaggcg atattcaagc taaagaacct gttttgacaa ccaatgattt
     9661 ggctaaaata aatcttcata aacagattat tgaagacttt gaaaaaaatc tttcaacaac
     9721 tgaaatttta aaaaaataca atattccgag aggaaagcta acatcgactc tttttagaga
     9781 atatggagaa tcggcagtta ctaaagcaaa ggaagtagcg aatgagagaa aaaaacaaaa
     9841 atagtagtcg atacttagta gcgactgtag tttttgtttg tgtattgatt ttcctgtttg
     9901 attttttaca gattggaaaa agtataattg gaaatacatt gagtgcaaag gaaatggttg
     9961 ctttaggtgg tttgtatgaa ggacatttgt tcccgaatat attcatttct atcgtttctc
    10021 atattaactt gtaccattta atttctaatt tattgttctt agtagttctt gaagaagcac
    10081 tgaatgatca gtatgagtgg ttaacattat tgatcgtttt aatcatatca ggatttctag
    10141 gaagcttagt tccttttctg ctacaaagca acatcgtaag tatggggctt agtggaggta
    10201 tttttggatg gattgcttta tttgcgattg catacatcca ttttgatagc aaggaacatt
    10261 tattactaag tgtttttgta ttattgataa tcggcatttt attggtatct acttttgaat
    10321 caggcgaggc aaatgtcttg tcacatgtaa tcggatttgt ctgtggaatg atcttgggat
    10381 ttattctaaa gaacgtttca ttgatcaaac aaaagaatta aaagggagtg ctaaaaatgc
    10441 caaaaagaaa agaaaagaaa attccagtgt ttttgtctgt ctcagaaatg ttatcacttc
    10501 aaatactgtt aagaaaaaag attgaaacaa tagacgaaga agttagaaat aaaagggcta
    10561 tgtacgaaca acgtgaagcg ctaagagaac gaatccacta ttttgatgat atacacacta
    10621 aaatctatga agcacgtaca aatcacgaag acgaaagtaa attaggatta gctcaacaac
    10681 ggatacttga atggcttaat acgaatactg atcaagaacc gttaagagtg attagtaatc
    10741 tttttgaatt tggaacgaag cattggttta atgctgtaga gaaggatgct gacttacaag
    10801 aagcgtttga tacgctaagt aaggatagca aatccatgcg agatttattg attacttatc
    10861 taaatgagca tgagtttgaa caaacgaaaa ccaaaaagaa aaataaagaa tagaaagttg
    10921 tgatgaatca tgctattaac cctgttaggt tatttatgtt taattcttgc tttttttggg
    10981 gcaatcgcta taattattgg tcaatttcaa acaaataatc cgattatgcg taacataggc
    11041 attggaattg tgattgtaac ctttattgtg tttaatgcca ctaagatttt tggaagtaac
    11101 gataaacaat ttggtatatc gggagttaat aatagttatg ctccagattc tttgggaagg
    11161 gtagtaatag atggaacatt tgaaaatgta acgagttata aaaatgtttc gctgacagtg
    11221 aaggaagatg gcaagaaagc atcagacgac gttctcgcta ctattaagcc gtctcaatca
    11281 caatttgaag tgttttatac agttaaagag aatagccctg tacacaagct gacattggtt
    11341 tttaacgttg atggagaaga atactataaa gatgtaaaaa taaataaaac atcaacgaaa
    11401 aagattatcg aatcagcgaa aaaagaagta gctacagaag aaagtacaac tgaatcaagt
    11461 tctactgtcg aagcggaact accagataaa attgattttg atcctgaaaa agtcaaatcg
    11521 tttgaatctg tttctggttc tatggctgat cggtttaaca ctgaagtctt caaagctatc
    11581 aaaactgtta aattggatac tgaaaagcaa atgattaatg ttaccttatt caattcagtt
    11641 gaatctatgg atcaggataa aaaagttacg gttgcaagag aactaggaaa aagagtaatt
    11701 ggtttgtatc gtggtactgc taaaccagat tcagacggtg cagatttgcc agttcatgta
    11761 gtttatgaag acggtaaagt ttttggagat tctacagcaa acgaagcatt aacgattaaa
    11821 ttggctaaat agccagaaag ttataataaa caaatggtgt cacaccaata atatataggt
    11881 gtgacacctt ggagatgata gctgatggta gtaaataaag aaaaagttag aaagtatgtg
    11941 actttaaaaa tagcttctgt taaatattta gaaaagcgag ctaaagaaga agaaagagca
    12001 agaggtaaga aagtaacagt ttcagagttg ttagaagaat tgatcgagaa tgatcaaaca
    12061 ataagacgtg catttactaa taggtctatc agataacaat ataattaata aaaaagaaag
    12121 agtgatttgg tcttagacta aatcactctt tttatataaa gtttctttac actcattccg
    12181 aataatgtta aaattcttta tatgatatat agttccttgt cagaggaaga gataaccagc
    12241 aatattggag ggataataag tggaaaacaa taatgatatt agacatttta gcattgggga
    12301 aagagttaca acagtaagtg aattatcaaa ggattataga gataaagagg gaacgattat
    12361 ttctcttaat aagaatcgaa gaggcgtttg gcaatatgtt gttgatttag gtgatggcgt
    12421 tacagttgag tgtaaagata gtcaactgat cagaaataat ggcagtcatt ccattgaaaa
    12481 agcaattctt gtatacccaa aagtatcaag taagatcatg aatgactggt tgcttggaaa
    12541 atttggtgag tacaataaaa aagaaatcgc agaagtgatc ggcaaatctt atccaacagt
    12601 attagggtat ctaaaaaaga taacgagtaa agaagcaaaa attgaggatg ttttaacagt
    12661 aagagagctt caatcattaa tcgagtatcg gaataaacaa ataacaaatc ctgagtacct
    12721 tagaaaagaa gatggtctat tgaaacaatt ggaacaagta aataaaatcc ggaagatgca
    12781 aaaggtagct cctttaagcc ttgatacctt taaaaaaatc tatttgaaag agatcaaaaa
    12841 agactaatgt ggactgtaac tttaccgttc caacctgtat ctgcaccaag accaagtgca
    12901 agaaggaatg ctgctgatga aactaatgac gtagcagaga aaaggatttc aacctactat
    12961 gataaaaagt atcttgatta tttgaaaaaa ataaagagct ttatcgaaga tcaagattta
    13021 ttagatgatg aattttactc gattgtaaat gatcctatgg gcgcaattat ggaagtggac
    13081 ttttactacc agataccaaa aagctataaa aaagtctata acattatgaa gacaacagcg
    13141 ccagatttag ataatttagt aaaaagtgct atggatggta tttttaacat ctcagctata
    13201 cgagatagcc atatcacagg cttaattgct tttaaatata atgtggcgaa cgaaggaaaa
    13261 acagttgtga ggatcaaacg atattcagaa tttgagtggg atacttctga aggattgaac
    13321 ggagatatat gggaagctac atttgatttt aaaccagtag caacgccaag accaaagtct
    13381 aaatttagag gaaatgggat tatcacttac tatccaaaag agtacaacga ctatttggaa
    13441 aatatcaaga atacgcttaa agagaaagat ttggtaaatg atcaactgaa aaaggtaatg
    13501 aatgctccta tgggggttat agctcagata gattattttt accaagtgag aaaagataag
    13561 aaaaagcttg attcactgat gcgtacttca cagccggata ttgataattt agtcaaagga
    13621 acattggacg gaatttttaa caaggaaata ggcatcaaag atagcagggt tgtagggtta
    13681 attgctttta aatacaatac ttttgaaaag tcgaaaacaa acgtgagact acaggagatg
    13741 ggctgatgaa taataatgaa aaaattcttc atgtacttga tagctttgaa ataattcaag
    13801 aagaactaaa aaaatatcgg gatgttttgg aacaaagata tgattttgtc aatcagcaaa
    13861 agagtaatca catggatttc attctcaata tgaatgactt gaagaagaaa ttagttgaac
    13921 gaaaagaaca agaaaagttg atcaaagctt actttgaact cggtgaaaaa gaagttaaaa
    13981 atgcaatgga actgaatgaa gagcgcaggg ttttagatca gctgttagaa caattattgg
    14041 tgatgtttca aaaaggacga atagatgagg atttaattga agaaggattg agaaaatatc
    14101 cttcaaactc aggtatcgga atagtgctga aagcgattga tgaagaagaa attgaagctt
    14161 tcataccgcc agaggatttt gaatcggcaa tggaatatat aaaatattat agccaaggga
    14221 tcactgcttt tagagagttt gatcctgaag atgttattca tgacttgaac aacctaaaag
    14281 aatggtgtga aagttatgga gtcgatgatt ccggattgga ctacttgatc agtattatgg
    14341 aaatcgaaga agagatgccg gataagcctg atccaacaga tattttagaa ttgatccatg
    14401 aagctagaaa tccaattgcc tatatttcta gaggatatac tgtattggaa tactataagc
    14461 cttatatatc agcgatgaac catttaagac gtgtattaag ggaaaaacgt gaataccgct
    14521 cagtgttgaa tgcaacgaat agattagaaa aagctgtttc agagctagat gcttattatc
    14581 gtgaacatta cctgcaagca ggtggtatgc caagaaatac gaaagcaaat atcagtaggt
    14641 atataaaaaa agctgagtag gcggagttag cccataaaat ggcaaaaggt aacctgaaag
    14701 gggcattttg aatgaaaagg gatgtttttg aattgtacgt attagaacgt ctagaagacg
    14761 gcatgatggc acaagagata attgatgaaa ttgatgatat gatcagcaga tttggtcaat
    14821 tggagttgaa tgatggaaac aaaaattcgg tagtgctaaa acaattttgg ttgaacaatt
    14881 tttcgtataa ggattttatc aaagaaaagg caaatacatt gtactgacta aattcacgag
    14941 gagcagtgat gagagcttat tgctaagagc agttaggctc ttttttgttt gtgtaaaatt
    15001 atttgtttta taatacacat ttatacacaa aaaatattga cagatagtac acaaatgtgg
    15061 taggatatat ttgtaagcaa gttagattaa caagttaaag gaagagcagt tatgaaaaaa
    15121 gaagaactaa tagatatatt tcaaattgtt gaacgagcaa ataatatggg aattatgttt
    15181 tttgatcgta tttctttaaa gatggattta agcgttgccc accaagaatt taatctgcga
    15241 ttaaaagcct tgttgaattc tgatgatgtt aatttcgctc atgatgttgt aggtatacag
    15301 aaccatatag atagagaaaa caaacgtatg ggtgatgaat tcttaccaag atattcaagt
    15361 ttatgatttg gcgaaagatt caagagtatg tgtcattatt gataggtact cttgaattgg
    15421 aaaggaggaa tgaatttaag acaacaacga ttatgatctt aaaacaatcg tttatgtaat
    15481 tggcaatctt acgaataatt ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa
    15541 tcaaatgaaa aaatttttag taggaattct attcacaatg gggctagtat tattatcacc
    15601 ttcagtttca gcagaaagct ctatttccat gtatcgtatg tataatccca atagcggaga
    15661 gcatctatac actagaagcg ttggagaaag agacaatctg aaacgttatg gatggcatta
    15721 cgaaggtgtc gcatggcaag ctccaacaag tggtactcct gtttatcgtt tgtataatcg
    15781 gtataacggt gaacattttt acacagttga tgccggagaa agagataaga tttcacacac
    15841 aggatggacg tacgaaggtg ttgcgttcta ttcctacaac ggagcaaata gagttcctgt
    15901 aacacgttta tataacaaat acgtgaattg gcatcactac acgttagatc aaaatgaaaa
    15961 gaacgtaatt agtaaacaag gttggaagat tgaaggtaca ggctggtatg caatgccaaa
    16021 cagcgtacca aatcctacac ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt
    16081 atggcaagga aatacaatgt tatgcgaagg agcttcttta ttagaaggtt tacactacaa
    16141 aggtgtagcc acaaatcaaa gcttaaccag ttttgtaaac tatatgccaa aagctaatga
    16201 caataatcct tatcatggtt actcaggaga gtggcgtcat aacgttaatg gaacgtatca
    16261 aggcatgatg gctgacccag tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa
    16321 tatcactggt tcaggaacga atggaatcaa gaatgaaatt cgcaatggta atccggttgt
    16381 aacttggatt acttataact atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc
    16441 agtatggaat ggacatgtag ttctcgtgga tggcttcaga aatggtgcat accatgttgt
    16501 tgaccctgta tttggcgcaa aatggatcaa tgcaagcacg tatgagagag catttaatgt
    16561 gactggaatg gcggttgctg tacgttaatt gatagaatta tcaactaaat gaaataagag
    16621 cttaattact gaaatggtaa ataggctctt atttttttag aggaaatttt tgtaatacga
    16681 atctataaca aaaaatattg aatgtagtac acaaaagtgg tatattaaaa tcgtagagat
    16741 tatgcgaaga ggtgattttg atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg
    16801 agcgactttc cgagctaaag gatgagttag aagtaattca agaagaagag tacaaagaaa
    16861 atatacctaa aaatctacag ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc
    16921 aacttgaaga agctgttgat ggtttatccg acatattaga attgattgaa gatatagcta
    16981 gctagagatg attaatgaag atttcaggag tgagaataaa atgattgata tttcactgat
    17041 agagaagtta cttttctcaa ataagtacac cgcaaaagag ctggctacaa gatggcaaat
    17101 atccgaatca gctattaaag cctatcgtca aaataaggga agcgtaggat ggcgtgattg
    17161 gaaagggatc agcattggaa aagcagaaga cataatgaaa cgtgaaggac ttcgatccta
    17221 taccacttct gaaaaagaca aataacttaa tgtttgaaaa gataaaatat ctgtcttaaa
    17281 gctatgtcaa attcaaatgg tgaatgtttg ctaaatataa tgcaaaaaaa ggaagggaaa
    17341 tattaaatga taagcgaaaa tttgtcttat gagaataata aacatctagt taaagaaaat
    17401 aagaaagtag aagattataa gaacatgtat atagaaatga aaaaatcacc actgtgtatt
    17461 caatataaaa atgataattg gaagcaagta gtcgaatttg taaaacctca tagtatacga
    17521 agagtacgaa gggggaagaa aaaaagtgaa cttgtaaata gcggaagagc ggtaattaga
    17581 gttataacca aagattatcc agttgaatat ggagattata ttttaaggtt aagttatcat
    17641 gcttttgttg tgttgaaaga agagcatttc aacgagtttt tcaatttaaa aagtaagtaa
    17701 tcatattttt ttgtagacgt gtataaagtt aactaaattg cgaggtgatg acatggtagc
    17761 aaaaaagaat actctaaaag aagcttctga aaaatatcaa gatatgaaaa gacgaggatt
    17821 aataccgaaa gacaaaaaag gtatatggag agaatttgtt aaggaagtca gtagtgatcg
    17881 aagtggcggt gactttgttg gacgatatac agcaaaaggc tatagcaaac gattaaggat
    17941 cactgttgaa tatggaacga atgaatcagc tacagagctg aagcaaaggt acttagatac
    18001 acgaagggaa gcttcaaaaa aatataatga tttcaagagc agaggtctga ttcctaaaaa
    18061 gaaaaaggga atttggcgtg agtttttcaa ggaagaagat agcaatcgga ataagggaga
    18121 cttcattgga cgatacacag caaaaggcta cactaagaat ttagggatca aggttgaata
    18181 cggaactggt gaaagttcat gggaattgag aaatcgctat ttaaatacat tgttagagaa
    18241 atataaagca gaattattgg agttgttcga tgttaatgat tttatcgaga tggctgaaaa
    18301 gtatggtcat gaagaaggtt ttatgatcaa tgacattatg gttattgata attttacgga
    18361 tttattagca cttgcgaaaa aagcaaggga tttaagagaa acgcagatgt atctattcag
    18421 aaataaaaac ggaagaaacc ggagaactga atcgagtgag attgaagatt actacgatgc
    18481 ctttacacac gatctgggag cctttgtaga cgagataagt ggtgtaatga acaaatatag
    18541 ggtctagaat aaaaaagccg gagatgagca gataaatggg aaacaatagc atatacacac
    18601 ctgaaatgca gaataaaatt aaaagacttg tcggattagg gtggatgaca agaggaaaat
    18661 tagattcttt tagtacgatc aaatatcttt tgatggacat ttaccgtcag aacaagcaaa
    18721 caggaataca ttttttgggc tatgataagc cggaagatgc acctttcttt gttccaagtt
    18781 cagcctatag agccagtacc tccggtagca tgtataactt ggatttgttt gaggaaggag
    18841 caaagatgat cgaatatgat ttcaacgaag cctattcgag gatcatgaga aattatcctt
    18901 tgccgtcaaa cacttatttg aaagcaaaat ataatacaga tcaagctatt ttgagagcga
    18961 taaatagacc aaaagtaaga ccgtatgaaa atttaagaac gtatatgttc tttttggtta
    19021 attttgaagg aacagccaaa gaaggaacat atgctaatcc aaaatcacag tcgatgtttg
    19081 cggattatgg cgaaaatccc aaaacagcta atgagcctat ttggttatcg gagattgagc
    19141 taacacttat ccttgattat tatgatatta aagagatgga agtcctagat agccacacat
    19201 ttttgacgaa aaaaggatta ctcaaagaat attttgaaag aatcgagcca ttaaaaactg
    19261 atcctgatac gttgaagctt tataaaaatc ttagaacgaa agtatttggt gctatcggac
    19321 aaaaatcatt aagagaagga gaaacaagca ccttcgataa atacagtatg tataatcgag
    19381 cgtattcctc catggtagca ggcgtattta gagatcgtat ggttagatat gaacagaaat
    19441 acgttcatag cgcatatggg ctattaaaaa ttcgtactga tggattgtat ttcagatccg
    19501 aagtaccaga gtttgaagag ctgtataaac ttggagtagt taagaaaaaa gttcatacaa
    19561 tatccaaaga agaagtcgaa aaagctttag aatttaaaaa caaaggattt gaaaataagg
    19621 gctcctttgg gaaatctaaa gatgaagtta agggtgttgt gatagacgat gatttcgagt
    19681 ttgattttga ctaaatactc tttggaaaag atggtaggag gtaaatatga agaagaaaat
    19741 tgtggaagga aatgtaggtg ttattattct aaaagattca actgactatg ctaaatcaat
    19801 ggcactaaaa gacgaaaaag ttgcacgcat ggataaagat ttagttgaat ttctgatgga
    19861 tgatacagaa agattaaaag gggatcagtt tttgagttta atgaaagaca aaggcttaaa
    19921 cacatatggt ttatttgatt ataagaaatt agctatagag tgggtaaaag aaggagaaat
    19981 attttttatt gaagaatggg aaggtactga gaacttagtt cttcatcagt atcttttgaa
    20041 agcttaatct aagtaaaaca gttctgacta gttatgcttg tcagaatttc aaaggaagtt
    20101 ttccataacc atcatcattc aaaaataaag gatttgaaaa taagggttcc tttgggaaat
    20161 ctaaagatga agttaagggt gttgtgatag acgatgattt caagtttgat tttgactaga
    20221 tttttttgaa aggtttaaga gtaaacaagg taagaactaa gatcatgaag attgagagta
    20281 aagaagatag aggacgaatg tatgagaaca cactatattt atttagaaga taaaattgta
    20341 gatgaaattc cggcaaaaag tcgagaacaa gctagaaaat tatttattga aaaatacaaa
    20401 gagatcaatg gagatagtga agttccaaaa gtggtcgtaa cagttagaaa aaggcaaaca
    20461 aaaaagagaa gtacgtttca tgtcagccct gatatatata atcttataga gcgacataga
    20521 agtgaagata atatttctat ctctcaatat attgctacag ttattgatga attgatttca
    20581 ggaaagtatg actatgcttt tgattttgag ggtgaaaagg attacaaaag tgttgctgtc
    20641 tttcaatctg attttgagaa gctaaaaaac tatgctggca ggagaaatgt taattttgca
    20701 agactactag aagagttcta ctccgaagag ttgggagtag gaaatgaata caagagagaa
    20761 acttatgcga aattgtctca taagttatcc caagcgctta aagaagtgac agaagttaaa
    20821 gcggaactag atgctaaact tgaagaagtg aaaaaatatc aaacagagag gaagaacaac
    20881 atgaagaatc aagtggaatt tgacgaacag aaagaatttg aaaaagcaag aaaacgctat
    20941 aacaaaatta tgaatgaact tagctatgaa cacttaacag ttcatgaata attgagtgag
    21001 ggtacagaaa aatatacgat cgcagatatg gttaatgaag tggaaactct tttagcttct
    21061 ttctttgaaa atggtcacgt aaacgatgaa tggcgaaaag aagaacctaa aagggttaac
    21121 gcttatattg ctcgattaaa aaggtttatt gataaatata aggagaatag tgacaaattt
    21181 tctaaagata aataaggaga gagtgtgatt acatttgcca gaaatcgaag gaatattttc
    21241 gccaaaaata ggtaaaaaac agttagattt cagcagtttt ccgagcgtaa atagagatta
    21301 taatctaggt gatgaggtac agatactaaa tattgatttc aaagaactag acttcgctgt
    21361 tgctgtttcc aacaaaccca aggcattgga acgagtgaaa aaaatatacc aagagaatga
    21421 tcaaaatatc aaaaatatga tcaaaaagta tagacgagat aattcaaaag ttgaagacgt
    21481 tttattcaac ggtacggcaa aacatgattt gaatgtttat atggcattag gagtggcttt
    21541 gtactgtaat tcaattaatc agacagatat tttggttaat atcgtgagga atagctatca
    21601 aaaaatatta aaaataagta gcgaaacaaa cccttattat gcaatgaaat atcttgggga
    21661 taatttatta aatgataaaa aattgatgca caacactgaa gagctgttat ctaatttggc
    21721 tgcgattaac tttctattca aaacaggttc tgaagattat tttatagata acgtactaaa
    21781 aggtgcttac ggaacagcat tttatccgct ttacaatatg aaacagcata aagccgaaca
    21841 aaaggataac aaacttgaaa ttaaaaagat gaaagaacta ttaggtatag gatttgtaaa
    21901 acccaattca aaagtcacta ttgcttcgtt agcaactact aacaatgatg ggaaaatagt
    21961 aaatgggttt gacgattcag atatagatat attgtcaaaa tcatattttt ctttgtatgg
    22021 agattttgca gatttgatgg gtattgatag ttacttagtc aataattttg agatcaaggg
    22081 tagcgagctg aatgatattg tgtatttcgt tttaatctct ctatatgcaa ttgatcagaa
    22141 taccgagtac cgacaaattg actatttgct agttttttat agtttgttat atcagttcgt
    22201 tttaactcgg ttgtttaaaa atgctaagga agaattgatc ctgaattctg attatgaaat
    22261 agcaaaacaa attgcaagta ttagaaaaga aaacaaatct gaagtaatca aattaagaaa
    22321 agaattaagt attgcgagta aagaaaacgg tcggttagaa tctttagtca aatcggcaaa
    22381 aaaagaagag gaacttttga agcaagagat taaagaatta aaaggaatca gaaaagaact
    22441 tgaacagctg aggtttgaaa ataaagtatt aaagcaattg caggagcaag aacatagaca
    22501 atcattaaat ggtaaaactg aagagccttc actgtctgaa atgatagctt atctaaaaca
    22561 taaaaacatt cttttagtgg gaggacatgt aaattttcaa aataaattaa ctgaactatt
    22621 accaaatatt aaaatgtttg atgtaagcga aaataatgga aagatactta aaaatttgaa
    22681 tacgtgtgat gcgatttttc tatatacaga ttacttgaat catggtctat attatcgtgt
    22741 tatgagtgaa atttctaata aagataagat attttatttg gataatcatg tgaatatgaa
    22801 cttaacaatt aaacgtattt acgatctttt atcacttata taaagagaga agtgataaat
    22861 aggacacaaa atttggaaag taggtaagtc actatgagag aactaacttt atcttctaag
    22921 gaattagaaa agaaaactgc tttattaaac gaaatattta gtgctgattg tataatccaa
    22981 agtttaaaag aaagttcatt ttcaaaacta acagatcagg aaaaaatttc ttatatgcta
    23041 agtttagata atctaggaac taaatatgat gaatcagcaa gtagcgataa gcttttgatt
    23101 gaattagtat caagtcttag tagttttgga aaaatgaaaa gatttaatgt gatattagaa
    23161 atttttgctt ggttaatccc ttcatttatt tggttttttt atattttcat agttgaatct
    23221 gagttaatag ccaaggtctc tcttggactg ttaattccga tataaatagg ttcagcaatt
    23281 tactttaata acttgtatac acctattcaa ataaaaaagc agttattaca tgaaggatta
    23341 agtggtaata tgttaaaaag aattgcatgg gataaagata ataaaacaga tattgagctt
    23401 aaagaatata aatacaatta tttctcgaga tgtatagata gaaagttact gtttaaaaaa
    23461 gaaggattag aatttagttt tgatgaagat gaatttgaaa aattagtgat ggactggcgt
    23521 aagaatttac tggatacggt acaattctaa aagcaattga ggtcgaaatt ccgatacttg
    23581 aatcatgatc tatattatcg tgttatgagt gaaatttcta ataaagataa gatatttcat
    23641 ttggacagtc atgtgaatat gagcttaaca attaaacgta tttatgatct tttatatgtt
    23701 tataaacatt aatgaggtgc taaaattgac aacaataact atgacaaaaa aaatagaagt
    23761 atatgttgat ggatcacaag aagaaaggga ttcctttttt gatttttttt ggaaaaatag
    23821 tagagaatat gtaaaagcgt tgaatctggt atataaacag ctaatgtttg aagagttgat
    23881 aaaagatgca attaaagaaa tggatcaagg ttatgctgag agagaagaaa gtattcaaaa
    23941 tcggattgaa aagttagcat atactgatcc aatagatcaa aaaaaacttg ataaagcaaa
    24001 agctgaatta tctaaattga gaaatgaaaa aaataaagaa gcgagagaaa tattaacgca
    24061 agcgataggg ttgaagagtc aaacgagaac acgagatgta gcaaaaaaat tagactttga
    24121 ctttggagat tttgttgatt ccgcaaatat gaaagctagt aaagattttg gcaatgattt
    24181 tatcggaatt gtgaccggac aaagaacgcc tagaatatat aaaaatagaa acaaatatgg
    24241 attattacct tttagaggtc gagatattaa aatccataaa gatgataagt tttatattta
    24301 tttacctaaa ggctttaggt ttaaagtgaa tttaggtagc aagccaagaa aagcaagaga
    24361 tgttgcttca gttttacaac acattatgga tggcgattac aaattaaatc aaagtaatct
    24421 tatttttgat ggaagaaaat tattttttaa tctaacaatt tcttttgaaa aaagctataa
    24481 tgaaatggaa ttcaagaaag gtaatgaatt gatcgtaaac tttgcagatt tgtgtagcgg
    24541 tgaatgttta attaatgata aatctgtaat gtcttttggt gatagccaat atataagaaa
    24601 cttcaaaagt aaaattttag caatgaaatc aaaagaacaa agccgaggag tcttttctaa
    24661 gggcggtcat ggtcgaaaaa gaaaattaga tcaagatcga tgggaagcaa taaagaaaag
    24721 agaaagtaat tttgttaaaa cttataataa tcaatgttct gcaaaaatcg ttaaaactgc
    24781 tattcgcaat aaagctcaaa ttattcgtat cattgaacca gataaggctt atggcgaatg
    24841 ggcttactat caattgaaag aacaactagt aaacaaagga aaaagatatg ggatagaagt
    24901 cagaagttag taaattaaac aatcgttgat ttggcaacga aatagtgagg gaaaatccac
    24961 tcactgaagg gtaaactccg tcaagttgtg tgaccagacg gaaggacgaa tgttgttgag
    25021 gttttagaac catataaaac agcatgtttc tcaaattttt aggataaggg aaattgctag
    25081 cagtttgttt tagaaccata taaaacagca tgtttctcaa atgagtactt taaattaatt
    25141 gatggcgttc ctgctttaga accatataaa acaacatgga tttaaaacaa gaagtaagtc
    25201 agttctcctt ttactttagt aaggaaaaat aaagtattct caataaaact taaaataagc
    25261 aaacctttgt tttaaaatga ttgtcaagtt aaaaaaatct gggatcatat aaaatagtat
    25321 agttctcaaa tatgattaga aaccctatta aagagcaata ttaagccaac ttacttaaat
    25381 cgtttaatta agaacaataa tctcttttag tcagactttt agtctgactt ttttgtattt
    25441 ccaaaattta ttctttacaa aaacgaacat acgtttgtat aattacttgt gaatggggga
    25501 ataaaaatgt ttgattacag tagagaaccg gtgaatgact attttttaat cgacatgaag
    25561 tctttctatg ctagcgtgga atgtattgaa cgaaatttag acccaatgac tacaccgtta
    25621 attgtcatga gtagatcgga caacactggt tcaggtctga ctctagcttc ttcacctact
    25681 gcaaagaaaa aatatcatat tactaatgtt agtagagcta ctgatttacc ttatccatta
    25741 ccaaaagaac ttcatattgt tccaccacga atgaacttat atataaaaaa aaatatggaa
    25801 gttaatcaga tttttagaaa gtatgtcgca gatgaagatt tattgattta ttcgattgat
    25861 gaatcaattt taaaagtgac aaaatcctta aatttattta ctatataaat tgagttaaat
    25921 tgtattcgtt tttcctccta taggcgtata ctaaagaaaa cacttgagga gtggggaaca
    25981 tggaaacaga acgccaaatc cgtgaattgg aaaaagcgat gaatacgaca gacaaccgcc
    26041 ggttgtacga gcgtttcctg gcagtcaagt tggttcttga aggccacacc cggaaagaag
    26101 ccgggcacac catcggccgc gacgagcata ctgtcagcca ctatgtgaaa aactactgcc
    26161 ataatggaat ccaaggttta gtctccaaaa aacaaagcgg acgaccttca cggctgacag
    26221 atgaacagga agcggaactg gtccaaatcg tcgcctacca cactccggag gaagtaggtt
    26281 tcaaatcccg tgccaattgg acattggcga ttgtgtgtga cctgattctc cgggaatgga
    26341 gttatggata tacgcaaaaa ggcgtggcgg acctccttca ccggctagga ctaagctgga
    26401 cccgtccgac gtataccctc aaaaaagcgg attccaagaa acaacaggta tttttggaag
    26461 aaaccttccc gagcctaaaa aaaaattgct gaatagcgat attgaccacc tcctattcga
    26521 ggacgaaacc atgatccggg attaccaagc cattggctgc acctggtttc tccgcggcca
    26581 ccagcgaaaa atccccactt atggacaaca caaaggggta aaattgatag gcaccttgga
    26641 ttatgaaacc ggtcggatca tttgtacaga agaaggggca tacgatgcag cggccttcct
    26701 ccgttttctg gagcggacgt tgcgggaata ccccaccgga aaaatcgtga tggtattgga
    26761 taatgccctt atccaccacg ccaagctgat ccagtctttt cttgaagaaa acgctaggcg
    26821 cctggcactc atcttcctcc ctccctacag ccctcagttg aacctaatgg aaggcgtttg
    26881 gaagtggctc aaggaatcgg tcatcaacaa tgtttttttt gaccacgtcc agaaaatcaa
    26941 acagtcggtc cgcggtttct tagccgatgt aaacgagcgt ccccttgagg tgattgaccg
    27001 actgtgcgtg cggatgtaaa gtatttaatt caacctatat agttcaggaa caagaagcga
    27061 gagaagaaga gaattagcta aaataatcaa agatgagatt cagaaagaaa caggtctaat
    27121 agcaacagtc ggaataggag ataacccatt attagctaaa cttgcactgg acaatgaagc
    27181 gaagagtagt aaagaattta tagctgagtg gacttatgag actgtacctg aaaaaatatg
    27241 gaaaataaac gagatgactg acttttgggg cattggatca cgcatgaaaa aaagattgaa
    27301 tcatatggga attatgagta tagaagattt agcaaattgg aatccctaca caattaaaag
    27361 tcagttaggt gtaatcggac tccagttata tttccatacc aatggaattg atcgtacgga
    27421 tattgctatt ccaccagaac caaccaaaga aaagtcttat gggaattcac aagttctacc
    27481 aaaagattat acgaggagag aagaagtaga actagtagtc aaggagatgg cagagcaagt
    27541 agccattcga attagacaac acaattgcaa aactggttgt gtacgcttag caattggtac
    27601 gtcaattttg gaagaaaaat caggtttctc gcaccagatg aaaataccaa ttacagataa
    27661 tacgaaagaa ttacaaaatt attgtctttt tttattcaat aagttttatg aggggcagga
    27721 agtacgacat ataggagtaa cttatagcag attagtatac acagattccc tacaattaga
    27781 tttatttagt gatccaagaa aaaagattgc tgaagaaaat ttggatagga ttattgataa
    27841 gattagacaa aaatatggat ttacagctat tatacatgct tcatctatgt tagaaggtgc
    27901 aagaagtatt acgagaagca ctttaatagg aggacacgca ggaggattag gaggtttgca
    27961 taatgattga atataacgat agaggacgtt taaaatggtc gggattttat ttgtctgatc
    28021 atactgaaag aatcaatgca gacaatgtac aacgtgctaa tcataatttg gcaaaagaac
    28081 aaatgacgac tgaagagatt gcgaaaatac tgagaactgc tgtcataaaa aatagacccg
    28141 tctgcattca aaaagaggaa cgaaacgctg aaggttacta tccagatgat gtagtaggaa
    28201 aaattattgg atatgacgag cttggtgtat ttgtggaaga agaaaaagtt cactatgatg
    28261 aaattaggaa tgtttctttc tcggaattat taaaatggag tcagatatga acattattaa
    28321 aaaatacgaa ttcggttata ttacgtatga acagctaata gaggaattat ggggatatgg
    28381 tcaacaatta attaatgaag tggggattga ttgcttttgt ttctatattg aatccggagc
    28441 aggataccat aagaacagat actatatatt tccatatcca ccaaaatagt ctcttagata
    28501 tttttaaatt ttctccaaat actttcaatt attgtgaaat atttcccttt aattaatcaa
    28561 attttttata aaagtaatat ataaaataaa ttgcttgaag tattatattt agatatcata
    28621 atttatggaa gaaaaattaa ttaaaaagga tgagattata atggggttac tggttccgag
    28681 agctaatact tatactatac ctttaacgaa acgccaatta attgctaaaa gatatcaaag
    28741 gattactaga gccatcaata gagagttttg gaattctgaa agtgatactg ctcacagttt
    28801 atacgtaggt tcttatggga gaggaacggc aattagtact agtgatatag atataatagt
    28861 tgaattaccg atggcagaat ttgacagatt caaaaattat ttatcaaatg gcccgtctaa
    28921 gttattacaa gttataaaaa acgcatttca ggaaatacta ccaaatagtg atattagagc
    28981 tgatggacaa gttgttaaaa taaatttcca tgatggaatc aaatttgaaa tagttccagc
    29041 ttttaatgaa aaagattatt ggggtgagtc aaaagggttt atttatccag attcaaacat
    29101 gggagggaac tggaaagcta caaatcccaa aaaagagcaa gaagccatga aattaaagaa
    29161 tacaaaaagt aataatttat tatatgctac gtgtaaacat tttcgacatg tacgagatac
    29221 agaatttacg agttatcatc tttcaggaat agtaattgac agtttcgtat atgaggctat
    29281 gggaaattgg aaattcgttg aaaataacag tgggggtcaa aacattagtt ctgtatctta
    29341 tgagacagct ttattagaat actataactc acataaagtt atgggcggtt taaatttata
    29401 ctctcctgga agcaatcaat ttgttaattc tgatagtagt atcatatgtt tagaaaaagt
    29461 attaaaaaaa atagctttat aaagttttat ggaggaacaa catgggagac gaaaaaaagc
    29521 gcttggaagt tcaattacgt gaagcatatg gacgagtaac gtatacttac accactcatt
    29581 taaagcaagc cgataatatt atttctgaca ataaaatttt aaggtgtctt caaattggtt
    29641 tgtcagccct tactactgga ggggtactta gtaccattat tactgataat agtgagttaa
    29701 cctttgttgc tggattatgt tcaacaattt ctcttggatt aaacttatac tttaagaatt
    29761 ttgatcttgc tgaagaagct aaaaatcatc aaattgttgc cgataaatta tggttgatca
    29821 gagaagaata cgtaagctta ctaacagatt taaatcgttt aagtgttgaa gatattgtga
    29881 aaaaaagaga tcaattaatg aacgaaactc atgaagcata taaaaatgcc cctaaaacaa
    29941 atagtaaaag ctatgaatca gctcagaaag cattaaaatc tgaagaagag caatttttta
    30001 cagaagagga attggataag atgttaccag agcatcttag aaaaaagagt tctaaatatt
    30061 gaactctttt tttatgctta aaattctctt gtaatttttc tattcatcta tatttaagaa
    30121 gaaatatgat gatagaaaca tctcctaaag ccaccgcaag ccgttctaag catagcttag
    30181 cccgacattg ctagatatgc tcatctcacc atgaacgttt cctaaagcca ccgcaagccg
    30241 tcctagccat tgcctagtcc gacatcgcta gattagtgca tctcgccata aaagttttct
    30301 aaagccaccg caagccgttc taagcatagc ttagcccgac attgctagat atgctcatct
    30361 caccatgaac gtttcctaaa gccaccgcaa accgtcctag ccatcgccta gtccgatttt
    30421 gcttgatcgg ttccatttct gtttatttag tgggtcaaca ccgtaatctt aaatagccat
    30481 cgcctattta agattactag gtctgctttg aaggtaaaaa gtctttcatt cttcaagact
    30541 tttaggcttt caagctacgc ctgaaagcat tgaaattcat cgctctttat ttggttttgc
    30601 catttataat tccttacgcc cattatcttt ttcgcctgtc ataccttgtc agaagtgttt
    30661 agagcttatc tcatctttgg attaatacaa gtggcgtaaa tttttttcta aacgcaagcg
    30721 ttacgaaaat aatttactta aacacttgta ttaatccggc agataagaac gcattctaat
    30781 cactcccgac aaggcaagac aggcgaaaaa gataaataaa tgaacgaaag gaagaaacat
    30841 aaaatgcaaa accaaatagc gatgaagaaa aaaccacctc cctctaaaac aatccaaaca
    30901 aaaaagaatt ttgaaaaacc aaaaatcaaa aacagaaaga agcgttcaac atgagagata
    30961 gaattttaga agttaatcaa gaaatggtaa acaagttaac aaatatcagt gggaactatg
    31021 aaaagtacga tggtacttta cttgataact atatttttca cgatacacaa aatatcagaa
    31081 ttggcagaag taaaccgaga gcgttcattc tcatcaaaga gaaatatttg aatgaatgga
    31141 atagcaccta tgaaatggtt atgactgata actttgaaaa ggtattggat ttcagaaatg
    31201 ctttgttggg ttctgatatg ggaaatagca gtcttgaatc tattgaagtt gaaaacaaga
    31261 ccgagaatga aggtgaagga ttgattaaga atgtcttgaa aatcgaacaa aaatatatca
    31321 gtgaagatag aagtttttat agagtcacaa gctcggttgg tctagcaaac tttgctacta
    31381 acttaattgg aaacaatgct agtgaatctt tgctagtgat gtgtctgaat acaaagaatg
    31441 aggtcattgc ttatagcgaa gttttcaaag gtggaattaa tcaatcagta gcgataccaa
    31501 gagagatttt tcaactagct ttgctaaaca attcagcaaa aatcgcagta tcacataatc
    31561 atccgagcgg atatgaattt ccaagtgaaa atgacaaaag ttttactacg aatttgaaaa
    31621 acgcaggaaa aacaattggt attgaattgt tagaccatat catcgtaaca agtaaaaaca
    31681 caagctatta ctcatttcaa gaaaatggtt ctttataaaa attagagaaa agaggaaaac
    31741 aagatgggcg aaagaacaca agcgttaatt acagtcaaat atggtaacag caaaacgaaa
    31801 gcaagcgtgt cgattcatta tcagtggggg tatggtcgat atatgctgat ggacgtattg
    31861 aatcaaggaa tttatttaat caataaagca tacgacttac gagataaatc aagagaagaa
    31921 ttagcaaaag gaatctttga tagaagtaca ggagttatca attttgtcac ctatgaatct
    31981 ggagtgagtg cttatgatga aacagggttt ggtgggcgct atgaacctta tcaagaaacg
    32041 attgaagatg tgatgaagtt ttcagataac aatgatggct atgctcattt agagatggtg
    32101 ttcgatgaaa atggatttag tctagatgat aaaaccagat tgcgattgtt tgattttgag
    32161 ggtcaacaga taggactaaa caagttttgt gaaagcggaa aagagtttgc tacgaaagat
    32221 tttattaagg gatacagatt attgctagaa aattatggaa taaacctaga agaagagagc
    32281 gtgaaataag tgtttgaaaa aagaaaagac cagttcccta atttagaagc gaaaagtcaa
    32341 aaagtgattg atggtgaaat gattgaatta ttccaaaaaa gttatggaac catagaaact
    32401 accgcaagtt gcaaatacta ctttcaagga aagagaaaca cgtttaaagg ctttgccgat
    32461 tctgatgaag aattggcaaa gaaaatcatt gattggcttg tcagcagtga acacgtagca
    32521 agagaagata ccgttagaac aacgattaat ggggaagtcc taacaggtac tagatgtgtt
    32581 tgttgtaatc atgaatttgt agaagatgaa ggttgctacc gaatcaatga atatggtgat
    32641 tttatttgcc agagttgcca cgagatagaa tttccaaccg aaagaagttg gtttgaatac
    32701 atgctacaag attatgagat ggaaacagaa aatggtgaag tagttttttg taccaaagag
    32761 gaattgagta agagaagcga tgatgaaatt gaagattggt gtgtgtggtt agtagggaca
    32821 gatgattgcg ttgcatatta cacaacagca gaataggagt gtgaatcagc atggaaggaa
    32881 tggattttat agatcatgaa gatttgattg attttggtta tacatggaaa ggaatggtag
    32941 gtattagcag aagtcttgct aatgcctttt acgaaagaaa ttatgcagtc tatgtccttt
    33001 atgatgacaa tacagaaagc cttgttgatg aagagtataa attggattta gagaatgttt
    33061 tatatgggat agaaaaagaa gatttagcaa aatatatttt ttcatggttg ggtcaataaa
    33121 cgaaagaggg tagcaacatg acaaaattca aaagtaaagg tatgcaatgg tgttcttatt
    33181 attatgttgg gaatgatgaa gagcttttga gaaaagccaa acaaaaaggt tatccagata
    33241 agatgttaaa acagattggc tattacatgc cagaagagag aatagaaaaa gaatcttgta
    33301 aggttctttt tgatagtgaa cagctattag ttttgttaac tcaaaataaa ttgatttgtc
    33361 aatttgaaaa taattaatat ggggtgaaga accatgaatg agaataagat ggtattaagt
    33421 gaatacgcaa gattgagaac cattaattgg ttgaatggta tgcaaagtag tggcgctttt
    33481 gatggatttg atgaggagaa tatggacttt gaaagtttat cgaatgagga tattagatat
    33541 tattttatcg aatggtattt tacagaacca gatgaagagg tggaagatga accagaatta
    33601 attttagaac gtcaaagaat tttattcagt gaacaagata gtaagaggtt gtatggttta
    33661 tatgacttac aactgaaagg attaaaagcg cagtatgttg ctgatagtta cgatgatatg
    33721 gtagaacagg gggcagatag ggttgtcact tattcagaag atgatgaaga ggaatgggaa
    33781 aactattcag atgatgagat atgctttaat gatgggtata tgcctattga aatcaccgaa
    33841 gaagaatatg ataaagtgtc ggatagcgat gagattggta tgtgtacggt tgtaagtgga
    33901 ttggatatag atgtccaaag agtagaggtt gcataccaga agatgaaaga acgtataaaa
    33961 agtaaagtag aatcttgatt tgattggatt gtttgcaaag tagctaatta agagtataat
    34021 ataatcataa atgtagttaa tggatataat aatgagtata ttatactcaa aagaggtgaa
    34081 cgaaatgaag gtaaaaaatc caaaagaaaa aagcagaatt caagtcggaa tagacaagga
    34141 tttaaaagaa aacgcagaaa tgatattgga agagctaggg ttaaatccaa caacagcaat
    34201 cacaatcctt tataagcaag tggtcgctag aggtgagttt ccagtagaga taaagctaag
    34261 cgaagaggaa aagcaaggaa tcagattgca acagctaact atggatatgc cagttgatgt
    34321 tctagataca gatgaaaagt tagaagagtg gttcaatgaa gcctaatgag atagtaacgg
    34381 tctatgttgc atttacagat aaaaatggtg gtaaaagaag acctattttg gtggttagtg
    34441 ataaagaaga tagagtagaa ttttttggaa tcacaagtca atatgagaat aaatcagacc
    34501 aaataaagag agtgtatttt cctattgcag aatgggaaaa agtaggtcta aagaagcaat
    34561 catggattga cgtagggtct ttaaaggcta ttcccaaaag ccgagagaac atatcattta
    34621 aaaaggttgg tatgctatca gctattgata cgaaaaagct aaataggttt atcgagagat
    34681 tacaaagaaa agtctaacaa ggcttttctt tttttgttta tatgtatatt aagatatacg
    34741 tttatataaa cgtatttatg tatattaaaa tacacgtaaa tatatacgtg attataaaaa
    34801 ttaagaaaat attaagtatt gttaaatagt gataattagt aatatacgaa ttataagata
    34861 cattttatat ttaatgtatg taaataaata aaatgactga cggatattga atagtaataa
    34921 ataacaataa ttcagacgga tacagtgagt gaagtagctt gctacgaacg aacaggcaga
    34981 atgaaacggt agtttcatta gagcaagtcg cttgcgacgt agcgttacca ctgatgtaac
    35041 tggcaagtta catagagaga gtttgcttgc aaacgaacgt tacccacttg aatagaaaac
    35101 aaaaataaca tattagtgaa ttttgaatag gagccacaac tcaaccaacc gcttgcggtg
    35161 ggtgagtccc acggaggaaa agacgaacga acggtcgcgt agc
//
LOCUS       DANKAY010000017        34510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000017 DANKAY010000000
VERSION     DANKAY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            212..604
                     /locus_tag="PWY98_000852"
     CDS             212..604
                     /locus_tag="PWY98_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2036621.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PWY98_000853"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PWY98_000853"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2036622.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PWY98_000854"
     CDS             3165..4775
                     /locus_tag="PWY98_000854"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2036623.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PWY98_000855"
     CDS             5067..5936
                     /locus_tag="PWY98_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2036624.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PWY98_000856"
     CDS             6101..7372
                     /locus_tag="PWY98_000856"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2036625.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PWY98_000857"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PWY98_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2036626.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PWY98_000858"
     CDS             8763..9026
                     /locus_tag="PWY98_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2036627.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PWY98_000859"
     CDS             9471..9920
                     /locus_tag="PWY98_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2036628.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PWY98_000860"
     CDS             9977..11395
                     /locus_tag="PWY98_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2036629.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PWY98_000861"
     CDS             11395..12255
                     /locus_tag="PWY98_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2036630.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PWY98_000862"
     CDS             12429..13202
                     /locus_tag="PWY98_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2036631.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PWY98_000863"
     CDS             13349..13714
                     /locus_tag="PWY98_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2036632.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PWY98_000864"
     CDS             complement(13862..15070)
                     /locus_tag="PWY98_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2036633.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PWY98_000865"
     CDS             complement(15089..15367)
                     /locus_tag="PWY98_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036634.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PWY98_000866"
     CDS             15521..16216
                     /locus_tag="PWY98_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2036635.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PWY98_000867"
     CDS             complement(16658..17455)
                     /locus_tag="PWY98_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2036636.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PWY98_000868"
     CDS             complement(17452..18636)
                     /locus_tag="PWY98_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2036637.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PWY98_000869"
     CDS             complement(18661..19908)
                     /locus_tag="PWY98_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2036638.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PWY98_000870"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PWY98_000870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2036639.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PWY98_000871"
     CDS             21888..22202
                     /locus_tag="PWY98_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2036640.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PWY98_000872"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PWY98_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2036641.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PWY98_000873"
     CDS             22887..23531
                     /locus_tag="PWY98_000873"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2036642.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PWY98_000874"
     CDS             23546..23875
                     /locus_tag="PWY98_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2036643.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PWY98_000875"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PWY98_000875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2036644.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PWY98_000876"
     CDS             24863..25687
                     /locus_tag="PWY98_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2036645.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PWY98_000877"
     CDS             25680..26027
                     /locus_tag="PWY98_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2036646.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PWY98_000878"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PWY98_000878"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2036647.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PWY98_000879"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PWY98_000879"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2036648.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWY98_000880"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWY98_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2036649.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWY98_000881"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWY98_000881"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2036650.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PWY98_000882"
     CDS             31657..32451
                     /locus_tag="PWY98_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036651.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PWY98_000883"
     CDS             32441..34483
                     /locus_tag="PWY98_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036652.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11094 a         6120 c         7248 g        10048 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg tataagacaa
    34501 gataataaat
//
LOCUS       DANKAY010000018        33924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000018 DANKAY010000000
VERSION     DANKAY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1152
                     /locus_tag="PWY98_000884"
     CDS             <1..1152
                     /locus_tag="PWY98_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDL2036653.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="PWY98_000885"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="PWY98_000885"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2036654.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="PWY98_000886"
     CDS             2728..3003
                     /locus_tag="PWY98_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2036655.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="PWY98_000887"
     CDS             3009..3776
                     /locus_tag="PWY98_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2036656.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="PWY98_000888"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="PWY98_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2036657.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="PWY98_000889"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="PWY98_000889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2036658.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="PWY98_000890"
     CDS             7920..8222
                     /locus_tag="PWY98_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2036659.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="PWY98_000891"
     CDS             8225..9388
                     /locus_tag="PWY98_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2036660.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="PWY98_000892"
     CDS             9414..12842
                     /locus_tag="PWY98_000892"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2036661.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="PWY98_000893"
     CDS             12863..13987
                     /locus_tag="PWY98_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036662.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="PWY98_000894"
     CDS             13977..14414
                     /locus_tag="PWY98_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2036663.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="PWY98_000895"
     CDS             14398..14736
                     /locus_tag="PWY98_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2036664.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="PWY98_000896"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="PWY98_000896"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2036665.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="PWY98_000897"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="PWY98_000897"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2036666.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="PWY98_000898"
     CDS             15893..16936
                     /locus_tag="PWY98_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2036667.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="PWY98_000899"
     CDS             17095..17823
                     /locus_tag="PWY98_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2036668.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="PWY98_000900"
     CDS             17939..18184
                     /locus_tag="PWY98_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2036669.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="PWY98_000901"
     CDS             18181..18399
                     /locus_tag="PWY98_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2036670.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="PWY98_000902"
     CDS             18535..19074
                     /locus_tag="PWY98_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2036671.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="PWY98_000903"
     CDS             19067..21340
                     /locus_tag="PWY98_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2036672.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="PWY98_000904"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="PWY98_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2036673.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="PWY98_000905"
     CDS             complement(22513..22767)
                     /locus_tag="PWY98_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2036674.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="PWY98_000906"
     CDS             complement(23167..23436)
                     /locus_tag="PWY98_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036675.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="PWY98_000907"
     CDS             complement(23906..24361)
                     /locus_tag="PWY98_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2036676.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="PWY98_000908"
     CDS             complement(24499..26427)
                     /locus_tag="PWY98_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2036677.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="PWY98_000909"
     CDS             complement(26624..27457)
                     /locus_tag="PWY98_000909"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2036678.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="PWY98_000910"
     CDS             complement(27649..28353)
                     /locus_tag="PWY98_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2036679.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="PWY98_000911"
     CDS             complement(28376..29002)
                     /locus_tag="PWY98_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2036680.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="PWY98_000912"
     CDS             29328..31703
                     /locus_tag="PWY98_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2036681.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="PWY98_000913"
     CDS             complement(31727..32218)
                     /locus_tag="PWY98_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2036682.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="PWY98_000914"
     CDS             32405..33325
                     /locus_tag="PWY98_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2036683.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="PWY98_000915"
     CDS             33479..33823
                     /locus_tag="PWY98_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2036684.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        11247 a         5931 c         7037 g         9709 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaat
//
LOCUS       DANKAY010000019        33528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000019 DANKAY010000000
VERSION     DANKAY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..779)
                     /locus_tag="PWY98_000916"
     CDS             complement(<1..779)
                     /locus_tag="PWY98_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2036685.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIM"
     gene            950..1318
                     /locus_tag="PWY98_000917"
     CDS             950..1318
                     /locus_tag="PWY98_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2036686.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1488..1919
                     /gene="mraZ"
                     /locus_tag="PWY98_000918"
     CDS             1488..1919
                     /gene="mraZ"
                     /locus_tag="PWY98_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2036687.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1940..2899
                     /gene="rsmH"
                     /locus_tag="PWY98_000919"
     CDS             1940..2899
                     /gene="rsmH"
                     /locus_tag="PWY98_000919"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2036688.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2904..3305
                     /gene="ftsL"
                     /locus_tag="PWY98_000920"
     CDS             2904..3305
                     /gene="ftsL"
                     /locus_tag="PWY98_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2036689.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3306..5498
                     /locus_tag="PWY98_000921"
     CDS             3306..5498
                     /locus_tag="PWY98_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2036690.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5524..6486
                     /locus_tag="PWY98_000922"
     CDS             5524..6486
                     /locus_tag="PWY98_000922"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2036691.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6500..7861
                     /locus_tag="PWY98_000923"
     CDS             6500..7861
                     /locus_tag="PWY98_000923"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2036692.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7887..8975
                     /gene="murG"
                     /locus_tag="PWY98_000924"
     CDS             7887..8975
                     /gene="murG"
                     /locus_tag="PWY98_000924"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2036693.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8990..10210
                     /locus_tag="PWY98_000925"
     CDS             8990..10210
                     /locus_tag="PWY98_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2036694.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10366..11691
                     /gene="ftsA"
                     /locus_tag="PWY98_000926"
     CDS             10366..11691
                     /gene="ftsA"
                     /locus_tag="PWY98_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2036695.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11714..12955
                     /gene="ftsZ"
                     /locus_tag="PWY98_000927"
     CDS             11714..12955
                     /gene="ftsZ"
                     /locus_tag="PWY98_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2036696.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12972..13649
                     /locus_tag="PWY98_000928"
     CDS             12972..13649
                     /locus_tag="PWY98_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2036697.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13663..14262
                     /locus_tag="PWY98_000929"
     CDS             13663..14262
                     /locus_tag="PWY98_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2036698.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14277..14564
                     /locus_tag="PWY98_000930"
     CDS             14277..14564
                     /locus_tag="PWY98_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2036699.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14613..15395
                     /locus_tag="PWY98_000931"
     CDS             14613..15395
                     /locus_tag="PWY98_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2036700.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15416..16123
                     /locus_tag="PWY98_000932"
     CDS             15416..16123
                     /locus_tag="PWY98_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2036701.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16385..19183
                     /gene="ileS"
                     /locus_tag="PWY98_000933"
     CDS             16385..19183
                     /gene="ileS"
                     /locus_tag="PWY98_000933"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2036702.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19232..20758)
                     /locus_tag="PWY98_000934"
     CDS             complement(19232..20758)
                     /locus_tag="PWY98_000934"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2036703.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(20773..21420)
                     /locus_tag="PWY98_000935"
     CDS             complement(20773..21420)
                     /locus_tag="PWY98_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2036704.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21604..21933)
                     /locus_tag="PWY98_000936"
     CDS             complement(21604..21933)
                     /locus_tag="PWY98_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2036705.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22110..22838
                     /gene="sfsA"
                     /locus_tag="PWY98_000937"
     CDS             22110..22838
                     /gene="sfsA"
                     /locus_tag="PWY98_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2036706.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            22854..23867
                     /locus_tag="PWY98_000938"
     CDS             22854..23867
                     /locus_tag="PWY98_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2036707.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            23867..25144
                     /locus_tag="PWY98_000939"
     CDS             23867..25144
                     /locus_tag="PWY98_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2036708.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25207..27909
                     /locus_tag="PWY98_000940"
     CDS             25207..27909
                     /locus_tag="PWY98_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2036709.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28061..28378
                     /locus_tag="PWY98_000941"
     CDS             28061..28378
                     /locus_tag="PWY98_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2036710.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28408..28728
                     /locus_tag="PWY98_000942"
     CDS             28408..28728
                     /locus_tag="PWY98_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2036711.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            28836..30296
                     /locus_tag="PWY98_000943"
     CDS             28836..30296
                     /locus_tag="PWY98_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2036712.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30364..30585
                     /locus_tag="PWY98_000944"
     CDS             30364..30585
                     /locus_tag="PWY98_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036713.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30616..30798
                     /locus_tag="PWY98_000945"
     CDS             30616..30798
                     /locus_tag="PWY98_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2036714.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            30798..31211
                     /locus_tag="PWY98_000946"
     CDS             30798..31211
                     /locus_tag="PWY98_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2036715.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31334..32515
                     /locus_tag="PWY98_000947"
     CDS             31334..32515
                     /locus_tag="PWY98_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2036716.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(32586..32750)
                     /locus_tag="PWY98_000948"
     CDS             complement(32586..32750)
                     /locus_tag="PWY98_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036717.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            32904..32977
                     /locus_tag="PWY98_000949"
     tRNA            32904..32977
                     /locus_tag="PWY98_000949"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32938..32940,aa:Arg,seq:cct)
     gene            complement(33046..33321)
                     /locus_tag="PWY98_000950"
     CDS             complement(33046..33321)
                     /locus_tag="PWY98_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2036718.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        11200 a         5715 c         7101 g         9512 t
ORIGIN      
        1 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
       61 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
      121 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
      181 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
      241 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
      301 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
      361 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
      421 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
      481 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
      541 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
      601 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
      661 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
      721 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
      781 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
      841 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
      901 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
      961 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
     1021 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
     1081 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
     1141 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
     1201 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
     1261 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
     1321 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
     1381 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
     1441 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
     1501 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
     1561 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
     1621 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
     1681 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
     1741 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
     1801 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
     1861 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
     1921 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
     1981 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
     2041 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
     2101 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
     2161 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
     2221 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
     2281 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
     2341 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
     2401 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
     2461 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
     2521 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
     2581 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
     2641 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
     2701 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
     2761 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
     2821 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
     2881 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
     2941 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
     3001 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
     3061 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
     3121 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
     3181 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
     3241 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
     3301 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
     3361 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
     3421 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
     3481 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
     3541 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
     3601 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
     3661 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
     3721 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
     3781 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
     3841 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
     3901 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
     3961 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
     4021 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
     4081 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
     4141 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
     4201 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
     4261 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
     4321 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
     4381 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
     4441 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
     4501 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
     4561 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
     4621 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
     4681 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
     4741 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
     4801 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
     4861 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
     4921 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
     4981 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
     5041 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
     5101 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
     5161 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
     5221 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
     5281 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
     5341 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
     5401 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
     5461 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
     5521 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
     5581 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
     5641 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
     5701 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
     5761 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
     5821 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
     5881 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
     5941 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
     6001 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
     6061 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
     6121 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
     6181 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
     6241 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
     6301 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
     6361 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
     6421 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
     6481 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
     6541 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
     6601 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
     6661 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
     6721 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
     6781 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
     6841 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
     6901 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
     6961 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
     7021 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
     7081 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
     7141 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
     7201 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
     7261 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
     7321 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
     7381 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
     7441 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
     7501 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
     7561 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
     7621 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
     7681 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
     7741 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
     7801 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
     7861 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
     7921 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
     7981 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
     8041 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
     8101 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
     8161 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
     8221 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
     8281 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
     8341 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
     8401 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
     8461 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
     8521 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
     8581 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
     8641 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
     8701 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
     8761 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
     8821 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
     8881 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
     8941 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
     9001 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
     9061 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
     9121 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
     9181 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
     9241 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
     9301 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
     9361 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
     9421 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
     9481 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
     9541 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
     9601 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
     9661 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
     9721 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
     9781 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
     9841 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
     9901 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
     9961 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    10021 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    10081 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    10141 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    10201 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    10261 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    10321 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    10381 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    10441 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    10501 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    10561 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    10621 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    10681 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    10741 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    10801 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    10861 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    10921 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    10981 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    11041 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    11101 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    11161 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    11221 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    11281 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    11341 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    11401 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    11461 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    11521 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    11581 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    11641 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    11701 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    11761 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    11821 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    11881 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    11941 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    12001 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    12061 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    12121 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    12181 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    12241 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    12301 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    12361 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    12421 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    12481 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    12541 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    12601 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    12661 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    12721 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    12781 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    12841 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    12901 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    12961 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    13021 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    13081 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    13141 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    13201 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    13261 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    13321 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    13381 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    13441 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    13501 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    13561 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    13621 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    13681 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    13741 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    13801 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    13861 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    13921 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    13981 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    14041 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    14101 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    14161 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    14221 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    14281 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    14341 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    14401 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    14461 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    14521 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    14581 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    14641 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    14701 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    14761 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    14821 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    14881 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    14941 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    15001 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    15061 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    15121 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    15181 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    15241 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    15301 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    15361 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    15421 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    15481 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    15541 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    15601 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    15661 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    15721 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    15781 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    15841 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    15901 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    15961 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    16021 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    16081 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    16141 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    16201 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    16261 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    16321 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    16381 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    16441 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    16501 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    16561 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    16621 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    16681 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    16741 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    16801 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    16861 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    16921 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    16981 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    17041 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    17101 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    17161 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    17221 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    17281 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    17341 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    17401 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    17461 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    17521 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    17581 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    17641 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    17701 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    17761 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    17821 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    17881 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    17941 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    18001 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    18061 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    18121 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    18181 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    18241 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    18301 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    18361 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    18421 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    18481 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    18541 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    18601 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    18661 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    18721 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    18781 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    18841 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    18901 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    18961 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    19021 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    19081 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    19141 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    19201 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    19261 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    19321 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    19381 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    19441 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    19501 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    19561 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    19621 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    19681 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    19741 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    19801 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    19861 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    19921 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    19981 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    20041 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    20101 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    20161 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    20221 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    20281 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    20341 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    20401 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    20461 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    20521 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    20581 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    20641 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    20701 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    20761 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    20821 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    20881 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    20941 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    21001 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    21061 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    21121 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    21181 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    21241 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    21301 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    21361 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    21421 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    21481 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    21541 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    21601 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    21661 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    21721 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    21781 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    21841 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    21901 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    21961 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    22021 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    22081 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    22141 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    22201 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    22261 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    22321 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    22381 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    22441 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    22501 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    22561 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    22621 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    22681 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    22741 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    22801 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    22861 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    22921 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    22981 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    23041 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    23101 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    23161 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    23221 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    23281 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    23341 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    23401 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    23461 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    23521 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    23581 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    23641 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    23701 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    23761 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    23821 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    23881 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    23941 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    24001 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    24061 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    24121 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    24181 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    24241 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    24301 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    24361 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    24421 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    24481 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    24541 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    24601 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    24661 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    24721 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    24781 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    24841 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    24901 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    24961 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    25021 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    25081 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    25141 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    25201 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    25261 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    25321 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    25381 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    25441 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    25501 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    25561 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    25621 gcagatgcgc catttgtttc tttcaattgt gcggattatg atcaaaatcc gcaactgctc
    25681 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    25741 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    25801 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    25861 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    25921 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    25981 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    26041 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    26101 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    26161 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    26221 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    26281 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    26341 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    26401 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    26461 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    26521 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    26581 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    26641 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    26701 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    26761 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    26821 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    26881 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    26941 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    27001 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    27061 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    27121 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    27181 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    27241 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    27301 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    27361 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    27421 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    27481 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    27541 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    27601 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    27661 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    27721 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    27781 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    27841 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    27901 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    27961 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    28021 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    28081 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    28141 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    28201 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    28261 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    28321 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    28381 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    28441 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    28501 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    28561 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    28621 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    28681 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    28741 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    28801 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    28861 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    28921 gtgatgcttt tatcggcata ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    28981 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    29041 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    29101 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    29161 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    29221 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    29281 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    29341 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    29401 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    29461 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    29521 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    29581 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    29641 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    29701 atggatcaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    29761 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    29821 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    29881 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    29941 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    30001 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    30061 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    30121 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    30181 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    30241 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    30301 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    30361 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    30421 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    30481 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    30541 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    30601 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    30661 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    30721 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    30781 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    30841 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    30901 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    30961 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    31021 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    31081 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    31141 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    31201 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    31261 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    31321 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    31381 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    31441 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    31501 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    31561 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    31621 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    31681 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    31741 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    31801 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    31861 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    31921 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    31981 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    32041 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    32101 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    32161 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    32221 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    32281 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    32341 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    32401 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    32461 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    32521 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    32581 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    32641 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    32701 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    32761 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    32821 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    32881 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    32941 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    33001 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    33061 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    33121 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    33181 gctaagtata tttactcctt gataaagtaa aatagatgca tgcgtatgtc ttagtccatc
    33241 aataaacagt cgaagagaaa atagggaaaa attgattact aaagaaaaac ttactaggtc
    33301 gacggtgtag ttgattttca tacttcaata gattcgaatt aaacgatttc gattggctta
    33361 taccagtcct atccaacgaa agatatatcg ttcttaactc tagcggtata acttgaagct
    33421 ttacgtaaag ccaatgggaa tgaactggag atgacttttt attattttta gtaatagctg
    33481 atgggttaat acttacaact ttccttttca aagtaccata taaaaaaa
//
LOCUS       DANKAY010000020        33190 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000020 DANKAY010000000
VERSION     DANKAY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            161..1042
                     /locus_tag="PWY98_000951"
     CDS             161..1042
                     /locus_tag="PWY98_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036719.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1055..2665
                     /locus_tag="PWY98_000952"
     CDS             1055..2665
                     /locus_tag="PWY98_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2036720.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2742..3401)
                     /locus_tag="PWY98_000953"
     CDS             complement(2742..3401)
                     /locus_tag="PWY98_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2036721.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3684..4349)
                     /locus_tag="PWY98_000954"
     CDS             complement(3684..4349)
                     /locus_tag="PWY98_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2036722.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4496..5101)
                     /gene="dhaL"
                     /locus_tag="PWY98_000955"
     CDS             complement(4496..5101)
                     /gene="dhaL"
                     /locus_tag="PWY98_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2036723.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5129..6139)
                     /gene="dhaK"
                     /locus_tag="PWY98_000956"
     CDS             complement(5129..6139)
                     /gene="dhaK"
                     /locus_tag="PWY98_000956"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2036724.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6114..6560)
                     /gene="dhaM"
                     /locus_tag="PWY98_000957"
     CDS             complement(6114..6560)
                     /gene="dhaM"
                     /locus_tag="PWY98_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2036725.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6663..6983
                     /locus_tag="PWY98_000958"
     CDS             6663..6983
                     /locus_tag="PWY98_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2036726.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7095..7963)
                     /locus_tag="PWY98_000959"
                     /pseudo
     CDS             complement(7095..7963)
                     /locus_tag="PWY98_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8059..9363)
                     /locus_tag="PWY98_000960"
     CDS             complement(8059..9363)
                     /locus_tag="PWY98_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2036727.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9360..9869)
                     /locus_tag="PWY98_000961"
     CDS             complement(9360..9869)
                     /locus_tag="PWY98_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036728.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10044..12179)
                     /locus_tag="PWY98_000962"
     CDS             complement(10044..12179)
                     /locus_tag="PWY98_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2036729.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12262..13917)
                     /locus_tag="PWY98_000963"
     CDS             complement(12262..13917)
                     /locus_tag="PWY98_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2036730.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13919..14233)
                     /locus_tag="PWY98_000964"
     CDS             complement(13919..14233)
                     /locus_tag="PWY98_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2036731.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14403..14663
                     /locus_tag="PWY98_000965"
     CDS             14403..14663
                     /locus_tag="PWY98_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2036732.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14677..14991
                     /locus_tag="PWY98_000966"
     CDS             14677..14991
                     /locus_tag="PWY98_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2036733.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15721..16275)
                     /locus_tag="PWY98_000967"
     CDS             complement(15721..16275)
                     /locus_tag="PWY98_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2036734.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16401..18330)
                     /locus_tag="PWY98_000968"
                     /pseudo
     CDS             complement(16401..18330)
                     /locus_tag="PWY98_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18636..19352)
                     /locus_tag="PWY98_000969"
     CDS             complement(18636..19352)
                     /locus_tag="PWY98_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2036735.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19353..21176)
                     /gene="glpO"
                     /locus_tag="PWY98_000970"
     CDS             complement(19353..21176)
                     /gene="glpO"
                     /locus_tag="PWY98_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2036736.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21190..22686)
                     /gene="glpK"
                     /locus_tag="PWY98_000971"
     CDS             complement(21190..22686)
                     /gene="glpK"
                     /locus_tag="PWY98_000971"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2036737.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22822..24282)
                     /locus_tag="PWY98_000972"
     CDS             complement(22822..24282)
                     /locus_tag="PWY98_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036738.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24272..25567)
                     /locus_tag="PWY98_000973"
     CDS             complement(24272..25567)
                     /locus_tag="PWY98_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2036739.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25899..26576)
                     /locus_tag="PWY98_000974"
     CDS             complement(25899..26576)
                     /locus_tag="PWY98_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2036740.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26606..27697)
                     /locus_tag="PWY98_000975"
     CDS             complement(26606..27697)
                     /locus_tag="PWY98_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2036741.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27798..28232)
                     /locus_tag="PWY98_000976"
     CDS             complement(27798..28232)
                     /locus_tag="PWY98_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2036742.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28816..30273)
                     /gene="ascB"
                     /locus_tag="PWY98_000977"
     CDS             complement(28816..30273)
                     /gene="ascB"
                     /locus_tag="PWY98_000977"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2036743.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30270..32165)
                     /locus_tag="PWY98_000978"
     CDS             complement(30270..32165)
                     /locus_tag="PWY98_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2036744.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32223..>33190)
                     /locus_tag="PWY98_000979"
     CDS             complement(32223..>33190)
                     /locus_tag="PWY98_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2036745.1"
                     /translation="EKQAIENRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPV
                     SLLFHETEFLLTHIKQMKTRMDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRP
                     NLAESAYFVLYLAEALNPFRKQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEP
                     VYLFKNQSNKITEYDAILTTEQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVE
                     EKKKTKLLDHYYKEENQIKIHKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVET
                     KITEYQLEIPLIHQQRKIKRVFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT         9242 a         7089 c         5762 g        11097 t
ORIGIN      
        1 gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac
       61 taaaataata aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
      121 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
      181 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      241 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      301 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      361 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      421 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      481 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      541 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      601 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      661 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      721 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      781 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      841 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      901 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      961 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     1021 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     1081 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1141 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1201 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1261 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1321 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1381 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1441 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1501 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     1561 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1621 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1681 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1741 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1801 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1861 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1921 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1981 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     2041 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     2101 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2161 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2221 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2281 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2341 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2401 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2461 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2521 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2581 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2641 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2701 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2761 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2821 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2881 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2941 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     3001 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     3061 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3121 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3181 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3241 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3301 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3361 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3421 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3481 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3541 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3601 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3661 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3721 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3781 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3841 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3901 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3961 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     4021 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     4081 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4141 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4201 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4261 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4321 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4381 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4441 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4501 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4561 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4621 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4681 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4741 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4801 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4861 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4921 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4981 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     5041 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     5101 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5161 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5221 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5281 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5341 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5401 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5461 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5521 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5581 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5641 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5701 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5761 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5821 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5881 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5941 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     6001 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     6061 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6121 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6181 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6241 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6301 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6361 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6421 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6481 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6541 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6601 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6661 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6721 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6781 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6841 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6901 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6961 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     7021 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     7081 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7141 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7201 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7261 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7321 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7381 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7441 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7501 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7561 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7621 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7681 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7741 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7801 tccgaggcac cgtttcagca ccagaagagc ttatggttgt tgaaggttct gtttgagcag
     7861 gttcagaagt ttccgcaaaa gctgtctgtc ccaataagaa aatactggaa acagcaattg
     7921 gccagattaa cggcttcatt ctttttttca tcacttttcc catccttact actttttaac
     7981 taatttctta ttataagcaa aaatgtttgg aagaaccata aacacaacag gctgtttgtc
     8041 taaaaaacgg tgcatctttt atttttttgt acgttccggg cttttccatg tcccgcttcc
     8101 ataacctaaa atcgttttga ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac
     8161 tatccagaac aaaagcatat atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg
     8221 atcatctaca atcagcgaag ctagcaactg gaaaaatcct gcgaccattt caaagcagat
     8281 gaacacaaaa ctgaatgtaa aagtaatata aatctgttcg tagtttcctt gatatacata
     8341 atgcccgatt aatccagcaa aaagaactac ggaaatacaa aagaatattg accagacaat
     8401 actcaatgtt tgatcaatga agatgatcgt tcttccaata tgtttaaaag gatggagcat
     8461 cacttttttg aaattcgtca gccagacttc tgttccacct tttgcccaac gttttctgtg
     8521 tcgatatagc atcttcaacg tcacaggtac ttccatgaaa aagataatct caggagcaaa
     8581 aacagataac cagtcattta actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg
     8641 atcttgtctg aatccgccca catcgatcaa ggcttctttt cgatacatcg tatttgctcc
     8701 actgtacgca taaagaccac cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat
     8761 acttgagaat tcaaccgttt gagattttgc aatcaatttg gtacgatttt ggacatccat
     8821 atttgcagtg accgcagcaa tatgtcttgc accagggcga atgaaataat tcacatacct
     8881 aatcagtgca tctggctccg gtaccgtgtc tgcatcattg cttaaaatca atttcccttt
     8941 tgcaaaggca agtccgatat taaacgcgtg ggcttttcct ttattttttt cgatccgaac
     9001 cacacgaaga ttagcgtatt ttttcatcag tctagctaat atttctggtg tttggtccgt
     9061 tgaaccatcg tctgtcacta atacctcata gttgtgataa ttcaatttcg tcatcaaata
     9121 ctcgattgta tattcgatca caatttcttc attatgggct ggcaccatga tcgtgatgaa
     9181 cggttcgaca ctcaagggaa catctaccca ttctttttgc ttatgtttgt ataaaaacac
     9241 ataacaccag acaccaataa accatacaaa tccgcctaaa atcggataag aaacgagaaa
     9301 aaaatacata atagacaaac aagtatctaa gaataaatta cctgtatggg tgattgagat
     9361 catttcagtt ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt atctagattt
     9421 ttctcttcgg agacagaata aaaacggacc gtttctctct cttcttttgt tccaaaacgt
     9481 tccgtataca cttcttcaag taattcttgt ctttttttca atttttcatc ttcatattca
     9541 aaagatttat ttactttttt gtatagattt cgattgttcc acctagtcaa tacaaatgag
     9601 gtaatcagga aactgaaaaa aatcaataca aaaaagccga ttaagaaata cagcattttc
     9661 ttttctgata aaaaagcgta caaggaaaaa tccttggctc tttctggtct cgttaatggg
     9721 aaaaagaccc atataaaagg aagaacaacg atcatccatc ctaaaagagc cactaacgtt
     9781 tgccaaatct ttaaccagta attacctttt gaaaaatact tatctttcac caaaattgat
     9841 gcaacttctt ctcttgttac ttttttcata tcctcacctc acgttttttt gttacgacgt
     9901 aggagtttac catacatttc atttcttttc acataaagtg ttcctaaatt tttcctaaga
     9961 aaaagaacaa aacatttctc atcagcaaat gaaaaatatt ttgttctttt tatcagttgt
    10021 tctttttatt ttttatctct taattatgtt gctctttcca atacgacaat gcttttcatt
    10081 cctctagata tcgccgtgta catttgtctg cgtcttcttt gctcatctcc atcgttttca
    10141 aatggatata agattacatg atcaaattcc agtccttttg ctagactcaa aggaatgatt
    10201 tggcagtttt ctaatccata tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc
    10261 tcttctgttt gggtgataac tgccgctttt tctcctttta gagaaaccaa aatctcagct
    10321 agtattttca acaattcttc agaggatcga aaagagcggt attcgggttc ttttccttgc
    10381 ggtcggatcg atacgatgtc gatcttctct tgatccacag cataggtttt gaaaagctct
    10441 gtgattgctc cgctagaacg gtaactattt ctaagatcat accgatggat cggcagatgg
    10501 tagtcgtcga aacaacgcat aatatcagca aaagtagtgg aagaattaaa gattgcctga
    10561 ttctcgtcac ctactaaagt aaagtgagtt tttggaaata attcgatcaa tagacctaat
    10621 tgtgccaggg tataatcttg tacttcatca atcaatagat agcgaaatgc tggtacattt
    10681 accttttcaa caagtaaatg acgaatagct aatataatga ctgcttcatc aacagtacgt
    10741 gtgggttgcc aagcataagc tgagccatat cgtttttcat aaattctttc aaacaactga
    10801 tgctcatcca cccatgccat ttcttcgatt tgtctggtta tttttcgata ttttttttgt
    10861 aaaagtttcc ttgcataagc tacaatactt tgttccgaat catcagaaat caatttaccg
    10921 aataattcca actgcctatc ttctgtcaac gagagtacct gattctgcac agcaggactt
    10981 tttgcttgtt tcaaaagatg attcttccat tcgctcatca gtgccttttt cgttgcttga
    11041 agcctatcga tcaatttagg atgagcagga actttctcat atattttttc gatcgttttt
    11101 ttagaaagga ctgtttttcc atttttagta agtccacgga taaaattcgg atgatctaaa
    11161 aacaaaggat ctgattgttt tagattatcg ataaactttt tgctacgcaa acgttctgtc
    11221 tgctccgata cattttctcc cgtgatacgt ttgaaataag cctcttcacc ttctatttcc
    11281 tctgctgaaa gctgtgaaac cagctgtatg atggttagat tcagtggatt tctttcacct
    11341 aatgatggta ggacattcga aatatagtcg ataaaccgat gattaggtga tagaatcagc
    11401 aagtcatcgg atgtcatcgt atctcgatat aaatagagca gatacgcaat acgctgcatg
    11461 atagcagagg ttttaccact tcctgctacg ccatttacca agatattttg acttttcaca
    11521 tcccgaatga ttgtgttttg ttccgattgg atcgtagcag tgatagcctt catttcattg
    11581 gtctcatctt ggcctaagac ttctaataat acgtcatctt gaatagctac agacgtatca
    11641 aaaaacttga ccaaacgatc tctttccaaa agaaattgtc gtctttcttt aataaccgta
    11701 tcgattttat tttgacgaac cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt
    11761 tcggcaattg gtgaacgcca atcataaaca agattttcat ctgcatcatc tgtgaaattt
    11821 gctgtaccga tataaaagga ttcttttggc tcattatcca gaaagtctac aacgactttt
    11881 ccaaagtagg gacttttcaa taaacgttcg atctttgcta gctgattttc tgcgtttttc
    11941 tgtttgatat tcatctgatc gatttctcta tttttcattt caatcatcga aaaagtatcc
    12001 aggttatcta agtaactgtc aaaattcaat cgaagctcgc cagccatttc tttcaacgac
    12061 tggtttcctt ctatttttgc cgtatccatc gattcccgca gttcgttttt tttatcaatt
    12121 agcttttgat acacgttctt cagatatgct tcttctaact gtttttgtgt gttgaccata
    12181 cacattctcc tcttttttta aagtggtgga accgattagt ttacctcttt taagagtcga
    12241 atgcaagctt tttaacgagt atcatttgtt aggatatatc agttcttcag gtcggacagt
    12301 ttgatataaa gaaaatgcac catcaaggtt ttttacgtgg tagccagact gcttcaagat
    12361 tctttccgct agatagctac gtaatccgct atgacagctg acgatatatt cttgttggct
    12421 gtctaattca tttaatcggt cccgaagttc attcaaaggg atatgtttgg cttccttgaa
    12481 tttgcccttt tccaattctt ctgctgttcg cacatctaat aaaatttttc cttttgctag
    12541 ttctgtcggc aattcatgcc attgtacggt ttcactaagc ccttctgcaa tgttcattgc
    12601 tgcatatcct agcatgttta ctggatcttt ggctgagcca aatggcggtg catacgtcaa
    12661 ttctaattcc ggtaaatcga agatcgtcaa atgccctttg attgctgttg ctaaaatatc
    12721 gatccgttta tccacacctt ttgccccaac accttgtgca ccataaatct cgccagtctc
    12781 tggatggaag atcaacttca gtaaaatatc tgttgcttct ggataatagc ttgcatgatc
    12841 ttttcctgat acatgaatga cttcaaaaga cagttcgttt tctttggctg ttctttcact
    12901 taaaccggtg gatgcggcag tcattccaaa cgttcgtata attgctgttc caataccgcc
    12961 ttgatttctt cgagaaattc cggaaatcgt gtccgcaact tgtctacctt gacgattggc
    13021 tggtgaagca agagaaataa gtgcatcttt tccagtgatt tgctgtttta caacgatagc
    13081 atcccccacc gcataaatat caggaatatt cgtttgataa cgttcgtcta ctaggatacc
    13141 cccgcgtatg cctaattcga ttccagcggt cactgctagc tggttatcag gacgaacacc
    13201 aatggataaa ataactaaat cagcagggat ggtacgccca tcatccagtc gcaatcgttt
    13261 cccgtggtct tcaaatccag cgacagcatg agaagtaatc acttctactc cttgatgaga
    13321 caattctttt tctataaaag ctgccatttc ttcatccagt gttggaagaa tatgtggtgc
    13381 ttgttcgatc accatgactt ctaaacctct tctttttagg ttttctgcca tttccaatcc
    13441 gatgaatcct gcaccaacga tcacggcacg ctttggctgt ttttctaatg catgcatcac
    13501 tttatcgata tctggtacat ttctaataga aaaaacattg tcggcttctg ttatccctgg
    13561 aatcgatgga acaactggtt ttgcaccagg agataaaacc aatgcatcgt actgttcaat
    13621 gtgtttttga ccctcatgta cgacttctac cgttttgttt tcgggaaaga tggctgtcac
    13681 ttcatgattt ggacgcacat ctaaacaaaa acgagcggat aatgattctg gcgtttggac
    13741 aagaagattt tcccgttcac tgatttctcc cgaaagataa taaggcaagc cgcaatttgc
    13801 aaaagaaaca tacggtcctt tttcaaaaac aacgatttct gcatcttcca ttaatcggcg
    13861 aagccgtgta gcagcagaca ttccacctgc tacacctccg acaataacga ttttcatctt
    13921 atttccctcc tactgttcga tcaaaccatt gattcatgcc accacgtaca ttcactacat
    13981 cataaccaga tttctttaat tctttagctg cttgtttact cctcatccct gattgacaaa
    14041 tcacatagac ggtcttttct ttgtctccat catatctgtt tatactttgt aatggaacat
    14101 ttttagctcc ttttatatgc ccgccccggt attctgcagg tgttctaaca tcaagtaacg
    14161 ctggattttc caaaagcttt tcctttaatt ctgggactgt tatcgatttt attgattgaa
    14221 atagcaaatt cattttctgc acctcattta catttttata aatacctcat ggggtatatt
    14281 aaacaggagc tttattaata tgtcaaccag aatgactctt catttttttc actacttttt
    14341 atatttgcat aaaataccga taaggggtat tatctaatta taaaaataga ggagaatgtg
    14401 ttatggagat tgataataaa aatatcctga accgtttgaa acgtacagag ggacaaattc
    14461 gtggtatcca aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc actcaattaa
    14521 gcgctgttcg ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa
    14581 attgctttga gaaacctgat agtaatccag aagaacaagc ggctaaacta gaacaagcga
    14641 tcaagatgat tattaaaaag taaaggaatg atttcaatga aagtcgtttt ccatattgat
    14701 gaattagaaa aatggtcaga aacagggaaa aacgtaaaaa acctgataaa agcttctcca
    14761 gaaacgacga ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct agactctgcc
    14821 aatgcagaat tcttagattt gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt
    14881 gctaatcata tttcagaaag ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc
    14941 cttgatttag ttgaactgca atcccaaggt tatgcttata tcaagcctta gtcagtagag
    15001 gttgcgacag aagtgatcag cttcaagaaa tttcggctta tttttcgaag aagctacttc
    15061 tggaaaaccg tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc
    15121 caaacaaaaa tagcttctca tatttttcgt ttggttgggc ttattaccgc agatgcaagc
    15181 ttttgaacac cgtttaagga agaggttgtg aaatcagcgg tttgacctaa gtattagaga
    15241 aaaaaacggg aaaatagctc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
    15301 ctgattgaac accgtttaag gaagagattg tgaaaaagtc gcttagtttc gagtattaag
    15361 aaaaattttg tgaaaatgga tttctacatt tttgcataaa tttggcttaa taccgagaaa
    15421 ctggcttttg aataccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt
    15481 agagaaacaa acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag
    15541 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca
    15601 caagaaataa tttcaaaaac aacttctcct gtttttggtt cggttggctt attatcgaga
    15661 agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt
    15721 ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag
    15781 accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag
    15841 cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc
    15901 tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc
    15961 ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa
    16021 aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt
    16081 tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac
    16141 aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag
    16201 caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc
    16261 tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt tattttagct
    16321 tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag acactgatat
    16381 tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt
    16441 cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca
    16501 cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg
    16561 tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt
    16621 ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt
    16681 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctgacgttc ttttttattc
    16741 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
    16801 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
    16861 gcaatccaca aagcttggct ttctcctgtt tcttccaacc cgcaactgat atttaggacg
    16921 aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa
    16981 tttcttgatt tctccataat gggtcgtggc aatgatcaat gccccttgct catacataga
    17041 ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc tctgttccac tgccaatctc
    17101 atctaaaagt accaacgtat tcctttttat ctttttcaaa atagcagcga tgttttgcat
    17161 atgaccagaa aatgtactca acgcattttc taaattttgg tggtcaccga catcgacgaa
    17221 aatctcatca aagacagcaa gctctgtccc ttcttttgcc ggaacttgca taccgaacat
    17281 cgccatcaaa gtcaataaac cgactgtctt caacacgact gttttgcctc cggcatttgc
    17341 tccggtgata atcaagcctc gatagtcttt accgatttca acatccaatg gtaccgcatg
    17401 ctcaggtaaa aatggatgtc tgccttgctt gataatgata tgttccgatt tattgattct
    17461 aggtgtgctt ccattgattt ctcggctaaa ttttgcccga gcaaagatga tatctaacac
    17521 cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt tctgcgatag caccagtcaa
    17581 tgccgccagg acttgatatt cttcactgat ttcttcagct tttaacaact gatagtgttc
    17641 attcaattta gaaatcacag ttggttcaat aaaaaccgtc gttcttttat ttgattcatc
    17701 gataatcgtt ccatctattt tatttttata gctggctttg ataggaatcg tatagtattc
    17761 gcctttttga acaatcattg cttcttggat catttctttg tttttcggat gacgcaagaa
    17821 tttcagtaat tttgactgga tttctttttc gattagttgt aattgtttgc gtactttacg
    17881 caaattacgt gacgcgtcgt cactgacttt attatttttt attttctggt caatcaactc
    17941 ttctacattt atcaaatcag gaagatgttt gctataagca aacaacaagg gtgtctggta
    18001 ttgattctta tcaaaaaact ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc
    18061 gatcaaatcg gttggctgca gaatcaaccc ttttttgact tgttctgtca gtgcatcgat
    18121 cctcggcaac cccataaatg gaacatgctg actgctttct aagatcaagc gtgcttctct
    18181 tgtttcttct tgacgatcca agacagtttg taggtttgtc gagaccgtca tatcgctgat
    18241 tcgttttttg ctatagtttc caattgctct tgcttggatt tcctttttga tccgatcaaa
    18301 ttgtaattga ctaatcattt cgttgtccat attctctcct tttcttgaag agatacctgt
    18361 cccaaaataa ataagggcct agagataaat aaaaggatag aacacagctt aaaaaagtca
    18421 tgtcctatcc ttattagaat acaaagatat tgttttcctg acaggtaccg tttcataagc
    18481 acaccaaata aactttgttt attggctatt cgacttactg attagatacc agctacactc
    18541 acaaaacata ccccattctt ctaggtctta tttattttcc acttaactat agcaagacac
    18601 attttggttg tcaaacttta gtatgtttta tagatttaca ccattaatga atagacacct
    18661 accgcgataa ttgctccgat cattggacct gcgattggta cccatgaata tgcccaatct
    18721 gaagttcctt tattagctat tggcaatacc gcatgtgcta atcgaggacc aaagtcccga
    18781 gcagggttga ttgcatatcc tgttggccct cctaatgaaa gaccgatggc taagatcaat
    18841 ccacctacag cgaatacgtt cgttccaggt gcaaattcgt tttgtccaaa agctagcaac
    18901 ccaagaacta atacaaatgt accaattaat tctgtgatca catttgctgg ataattccgt
    18961 atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt catctttcgt tgcattccaa
    19021 tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa aagctcctaa aacttgcgca
    19081 atgatgaaag gaacgacaag attccaacta attgcccctg tcatcgccat agctagagag
    19141 actgcaggat tcaaatgagc tgggcccatg aatccagaaa tatagacagc cattgttaca
    19201 gcgagtcccc aaccaaatgc gatgacaatc cagccagaag cctgtgcttt acttttgttt
    19261 aaattcacag ccgcacacac accatctcca agcaaaacta ggatcagtgt cccaataaac
    19321 tcccccatga tttgagtcat atcactgttc atttatttct ccccctttag ttcccgcaga
    19381 tctgattctg caatcaatgc ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc
    19441 cattcaaaat atccagcaat tgcatcgatc acaggttgct tgatctcatc aagctgatct
    19501 cgttcaaaca acagatgatt tgtacgacga atgagataat cgcctggggc caaaaccatt
    19561 tcttcttcta aaccataacg taaccgagct gactcagcga gactcaaacc aggataaggt
    19621 gccatttctt ttgctaattc aaagatacgt cgggcattag tcccgtaaaa atcagcgata
    19681 taagtagcat cttcttcctc taaaccggct gctactcctt cttttgctaa ttctgtcact
    19741 gtttcttcta atttagttgg atcgaagttt ccacctgaaa tctgatattt tttagagtcg
    19801 atttctttcg tctctattcc gtattcttct tttaataatt ggcgtatcaa tcgcaaagct
    19861 ccttctgcca tcttacggta atcggtgatt ttcccaccag ataaagtcag caaaccatct
    19921 ggttctcttt ctaaagaact acctctggaa atcgtagaag gtgctttttc atcacggctg
    19981 ttttccaaat ggttcaacac gtcttctact tcagcacgag aaactttgtt ttccttatat
    20041 tcacttactg tatcgacaac tttattgaag ctcttgtctg aaatcgatcc attatctcca
    20101 ccattgtaat cagaaccaga attaccaatc aacagtggac gaagtcctgc ccagcttgct
    20161 tcgatatcat tcaatgtgat atttgcttct ggatagcgat gattgatcac atccaataga
    20221 taatctacat cttcttgtgt gactttcgga tccgtaaaat ctccttggta atccgtatct
    20281 gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa aaaccatccg cccatcttgc
    20341 tttcctgtat cgaagtatgt gggttgcggt acaggcagtt ttttcgcatc tacaactaaa
    20401 tggatccctt tagttggacg cattttagga gagactggac gcgtaaaatt caagtttcgt
    20461 actttatcta cccaaggacc actcgtattg atcactaatt ttgctttgat ttcgatcact
    20521 tcatctgtca gcaggtcacg agctttgacg ccaacgattt gatcgccatc atataaaaag
    20581 ccaaccgctt tcattttact tactagataa gccccatctt cggcagcctt tttgatatta
    20641 tcaatcacta aacgggcatc attgttgcgg aaatctagat agacacccgc gccttttagc
    20701 ccttcttttt tcaaaaacgg ttctctttcc aatacttctt ctggtgtcag cgtataattc
    20761 tcatatttgg ttcctgtcac atttgccagt ttgtcataaa ggtccattgc tactttgacc
    20821 gagaacatat tgaaggttgt tgctccttcg ccctcataga ttggcaaaag cattggatct
    20881 ggttttggga tgtgtggggc aatactttgt acgactgcac gttcaccaac tgtgtcagct
    20941 actacttcca catcaaatgt cttcagataa cgaataccgc catgaacaag tttggtcgag
    21001 cgagaggaag tcccttctgc aaaatcctgc atttcgatca atcctgtttt gattcctgat
    21061 gctgctgact ggactgccac accggcacca gtgattccgc caccgatgat caacagatcc
    21121 aattcttctt gctgcatttt ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg
    21181 atttcctcct tattcaaatg cttgggtagc ttttactgct tttttccaac cgttgtaaag
    21241 tttttctcgg cgctcttcct ccatttgaac ttcaaatagc ttgccagctt cataaaattc
    21301 gcggatctcg tctgtatctt tccaataacc gacagctaat cctgctaaaa atgctgcccc
    21361 tagcgccgtt gtttccaagt tttctgctcg ttggatcggt acattcaaga tatctgtttg
    21421 gaattgcatc agatattcat tgtttgctgc accgccatct actttcaata caggaatctt
    21481 gattccagtg tcatcttgca tcgtatcgat gatatctctc acttgataag caatcgactg
    21541 taaagtagct ttaatgatat cttcttttgt cgtaccacgt gtcaagccaa acatagcccc
    21601 tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg aatgcaggaa caacataaac
    21661 ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat
    21721 ttgaagtcca tctctcagcc attgaatcga agaaccagcc acaaaaatac ttccttctaa
    21781 agcatagtaa acctttccat ttatgccgta accaattgtc gtcaataaat tatttttgga
    21841 taattgcggt ttttcaccgg tattcatgac aataaatgaa cctgtaccat aagtattctt
    21901 gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg gcttgctggt ctcctgccat
    21961 ccctgaaatt ggtgcttcgc ttccataaaa atgataacct tgcgtataac cataaacttc
    22021 tgagttagaa gtgacttttg gtaacatcac tcgaggaata tttagtaaat ctaaaatctc
    22081 ctgatcccaa tctagctgat ggatattgaa caacatcgtc cgactggcat tcgagtaatc
    22141 agtaacatgt gcttgtccat cggtcagttt ccatactaac caagaatcga tcgttccaaa
    22201 catcaattcg cctttttcag ctcgttcttg cgcgccttct acatgatcaa gaatccagcg
    22261 tatcttcgtt gctgagaaat aggcatcgat cactaaccct gttttttcgt ggaaaaattc
    22321 ttgatagcct tcatttttta agttatctgc aattccagct gactgtctgg attgccaaac
    22381 aactgcatga tagattggac gtcctgtatc tttttcccat attactgttg tttcacgctg
    22441 gttcgttatg ccaatcccag caatctgatt tggtttgatc cctgattcga tgaacgctcc
    22501 ggcaataact gactgtacag aattccagat ttcatttgca tcatgttcca cccaactttc
    22561 ttgcgggaaa tattgcgtga actctttttg agaactgccg atgtgtcttc ctttcttgtc
    22621 aaagataatt gctcgtgagc tagttgtacc ttggtcaatc gccatgatat acgttgattc
    22681 tgtcattgtg attccctcca ttatatgcaa acgctttctt taactactct tatcatacta
    22741 ctgttttaaa aaaagaaact gtcaaaattt tattcttttt ttcttttttt gacagttttt
    22801 tattatgaag ctatttttta atcaattctt ttcttttttc agcgtctcta ttcttctttt
    22861 caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga taatattcac actgtttttt
    22921 gtttctctgg caaaatgtga tcaccaaatc atattcttta ccttcttcat aacttccaat
    22981 ctcaataccg ccgattcccc tcagttctct gatcagatat tgttgaaaag caatacgata
    23041 aatcggcaga ctttctaaat cgattccaat cactactggt tttgcaatat aaaaatcgat
    23101 catcaaaaga acattggcat actggatcat caggtaacta agctcactat cttcagctct
    23161 tttcctcggc aattgctgca ccgctgtttc ctgtaatttc gccagtaact gtgttaagga
    23221 atgcccgagc attttctgct gctgttccaa aagccgatca cgctcataga cttcgatctt
    23281 tccaacaaag aaaaaccatt cattgttgat ctgggaaatt tggtagccta ctgccttttc
    23341 ttctttgatt ttcaaacgac gaggacgata atgccataac atcgtttccc gaatgtaagt
    23401 atctaaaaca gcggttggta actttttgct ccgtgtcaaa tcatactgat aaaatatttc
    23461 ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt
    23521 cagttctgct gctgttcgac taagatgcat cacgatgatt ggatcgattg cttgataaag
    23581 ccggtccgac tgatagagta atttcttttc tttcaaagtt gcgtacgtac ttttttcatt
    23641 caatagtctt tttttcgtaa tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt
    23701 aaacgttgtt tctaatacac ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt
    23761 ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg aatagttcat aataaaaata
    23821 tcgtatctgt agctcttctc cgtataattg accgtttttg atttgtaatc caaattcctc
    23881 taagagctgg ttcaattctt tgatctttcg aaaaacagaa gactcactga tattaaacgc
    23941 tgtcgctaat cgaacgatgg atacttgctg atgttctaat aaatagacga gcatcttaaa
    24001 cttcaagctt tcctgcaata aaaacaatag aattttttcc aaacttacat cttcgtccaa
    24061 cacaagcagc gcctgatact catttcgctg tacttctact tttttttcca tcattcggcc
    24121 aatcaattca atatcttcca aatattgatc aaacgaggct tttgacagtt ggacatgctc
    24181 tctcaaacca ttgatagagg ctgttccccc ttgcagaaca aggtttcgca aaatcaggat
    24241 ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc ataggcagta ttcgccacct
    24301 ttgaccacac catatctggc acacggtatt ctggctggaa aaatagctca tgttcttcta
    24361 actgttcaaa cgtccatcct tcttctactg caattgtcag ttggtcaaga aggtggaaca
    24421 tctctttttt gctttggagc tgtgctccaa gaatcttttt ttcagaatca aatagaaatt
    24481 tgagtttaac tttgctttga tttattgctg taagcttgta gtctacgtat cctgtttcaa
    24541 ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc
    24601 caaaccactg attgccgact ggacgatagg ttccctgatc ctttgtctgt gagccactaa
    24661 ttgtcttgct gacatttcgt gctgtacgga cagcgttatt tactagagaa acatacattt
    24721 tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc aatcgcataa acgtcttttt
    24781 gagacgtttg caagtagtca tctacccaga tcgttccatc atcattcaat gttaattgtt
    24841 cttcccacat ctgattgtcg ggacgagaat gattagccag caaaacaaga tcatagttct
    24901 cctttctttt ttccgtctct acaatagcgg tttcattttc ggtcaaagac acaacttgtt
    24961 cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt tactaacggt tctgtcattt
    25021 caggatctag ataacgaaaa agaattgact ttcggctttc gtaaagatgg atttgttttt
    25081 ttcttgatgc caacccttca gctatttcga ttcctgcttg tcctgcacca ataattgcaa
    25141 ttttctttgc attcatgatc ttttgctgaa gagccgttac gttttgtatc tttttaactg
    25201 ggtgaataag cgttgattct gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta
    25261 gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa aacgactttt ttttctttca
    25321 atcctattac tgtcttttct gtgtaaacat ggattcgata gcggtcagtc agttcttctt
    25381 tcgtgatcca agaaagttgt ttttcactgg taacctttcc ttttaatatc aaattgaacc
    25441 cgctgggaat ccatcctaac ttcgattgct tttcaatcaa atatatttca cttgttggca
    25501 tcaattggcg aagataaata gccgcatgaa tccctgcaaa cgacccgccg acaattacaa
    25561 tgttcatctg cattcctctt tcttataagg tgttctactg ctaacttctc aatcagaaaa
    25621 cacagtattc ataactcgct ttcaacctta tgatacatag cgatgcagca gaatcaaccc
    25681 tctccatcat accgtgttct aggtctgctt ctcaataatg tactagtgga cctaattgaa
    25741 gagtatcatt tctgaagaga tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc
    25801 cttttacgta cctctgtcca aataggaaac agaaaagaac gaaaagacga gttttagcgt
    25861 gtgtaatgta atagttagaa tagacacatc atttattttt aattctttaa tgcttcaaca
    25921 actgcttcgc ctaattgatg agcagattga ggattttgcc ctgtaagaag ccgcccatca
    25981 atttgaacca cttctgaaaa agctgctcca gactgatagt ttgctccttt ttctttcaaa
    26041 gcatcttctg ctaagaatgg tacttcattc gttgtaccat ttgcttcctc ttcttcatta
    26101 ctgaacccag tcactgtacg tccagctaaa atagattttc cagactcatc tgtaactggc
    26161 aacaatccta caacaccgtg acaaacagct gagaccagcc cttcatttcg atagatttgt
    26221 gaagcgactt tcccaagatc tattgcttct ggaaaatccc acattgttcc gtggccacca
    26281 gcataataaa ccgctgcata atcatttgca tccacatcat caggacgtaa ggtatttccc
    26341 aaggcccgat ttctgaaatc ttcatcttca taataggccc aatcgatttc atccatattc
    26401 aaaacacttt gcggttctaa aggtacatat ccgcctttag gactgacaaa atctgcatcg
    26461 atcccatttt ttactaatac atcataaaaa tgagtcagtt cacttaacca caaacctgta
    26521 ggacgctcga tcttttcgta cttttctaca tttgttaata cgatcaatac tttcattttc
    26581 ccatcctctt tctataaaaa ttgcgttatt gataacgtgc tttcaagaaa ttatataatg
    26641 ggcttggcaa ataaagctct ttatatgctt tttcaaaagg tttcgttaca aattcatcct
    26701 cacggttttc caataatttc tctgcccagt caggatcgac gatcatttgc tgtccaattg
    26761 ctgcaagttc tgcatatttc aatacctgct ctacatcttc gcgattgcgg atcccaccga
    26821 caccaactaa agggatcttt ccttgaatcg tgttatggac cacttcaaga atcgatttat
    26881 cgttgtattt atcagaacga gctattcgat catacatatt taatgaaaca tgcagataat
    26941 ctagttttga ctcacgtaat cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg
    27001 gtgtttcata ttcttctggt gagaaacgat accctacgat gaatggtttt ttagcatatt
    27061 gttcaaccgc tgagaaaacc gttgttaata attcttcaat aaaacgataa cgattttcca
    27121 agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg agagaaaaat tgttggatca
    27181 aatacgtatt tgctccatgc aactcgattc catcaaatcc tgattcaatc gctctacgtg
    27241 tggctttgcc aaatgcttgg attgtttcaa cgatttcttc ttcactcatt gctcgtggtg
    27301 tttccgcatc aggacgtttt gctgcaactg cacttgcaga tacaggctgt tctccagata
    27361 aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt
    27421 ctccttggat tgctttcgct agctcactta atcgaggaat catttcatca tgagcgatgc
    27481 tgagttcacc tggccagcct tttccgatat cttggacatt tgcagcacca gtaataacag
    27541 caccaagacc tttagaacgt tgtttataat aagtgatctc atcttcggtg atcactccat
    27601 tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa tcggttcttc aattgaactc
    27661 ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt cattcctccc ataattcgct
    27721 ctgattaacc tatcgagaat catctatcat taaaccactc gattagttgt acatgcaact
    27781 aatttgctta aaaaatttta ttttttttgt aacttatctg ttgcatcatt cattgtctgg
    27841 gctaattctt tacttgcttc atttcccaaa actgctttgt atgactgatg aagttcttcc
    27901 cagctaccat cgacttcctt agcaaattgg cgtccttgtt ctgtaagata aactaatgac
    27961 aagcgacctt cgctaactgt tttgacgtat cctttatatt ctaatttttc aacaaaacga
    28021 gcaatcgttg atggggcgga atagagcatt tgtgcaatat ctttttgtgg tattccatct
    28081 ttttctttta atatcaataa aataaaacca taagttggtg ccagacctgt ccttctaaac
    28141 gcttcatcag ctaatttatt caacatgcga tctagctttt tcacgctaaa gaaaagacat
    28201 tcatctaacg ctttttcaat tggcttttcc atttcatccc ctccattggc gtacatatca
    28261 tagtatgatt tttatttttt gtcaaaaaac acggaataat gtccgcctcc tctgtctaga
    28321 tttgaaaaaa agaagtagcc atcttcaaag aatttcggct tgttttcgac aaaactcctt
    28381 ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa
    28441 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg aaaaactggc
    28501 ttttgaacac cgtttatatc agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa
    28561 cagccaaaca aaaatagttc ctcatatttt gtcgtttttt gagctaatgc cgatggtcag
    28621 ctgattgtac accgtttatt gagaggtcgt gagcctgccg ttgaactccg agtcacaagg
    28681 agcaatttca aaaaacagct tctcatgttt tatgactatg gtgacttgtg atcgaggagt
    28741 tggcaggaga atactgttta ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc
    28801 ccttatttcc ttttattata ggatttcgcc atttgattgg atgacttgtc tgtaccaatc
    28861 aaatgatttt ttctttaacc gtttctttgt gccattccct tcgtcatctt gatcaacata
    28921 gatatatcca taacgttttg acatttgtga agtagaagcg ctgacgatat caatgcagcc
    28981 ccacgacgta taaccaaata aatctactcc atctttgata gcttccttca tctgctcaat
    29041 atgctgcttc aaatagtcaa tgcggtaatc gtcttgaacg gttccatctt cttctacttt
    29101 gtctatagct ccgagtccgt tttcagctat gaacagtggt ttttgatacc tatcatataa
    29161 ttgattcaaa gcaattctca aacccttagg atcaatctgc cagccccatt cacttgatgg
    29221 aagatacgga tttttaattc ctccagttat taagtttcct tggacccctt cccaattttc
    29281 tggatgagca ctgactgcac tagacatgta gtagctaaaa gaaacaaaat caacagggta
    29341 ggctgctaag atttcttcat cacctacttc tatttttaag ttgacacctt tttcttcaaa
    29401 cattcgttgc gtatacgctg gataatatcc tcttgcttga acatcactaa agaacaaaga
    29461 agttctttgc tgctcaattg ttttttgaat atcatccgga tcacaagaat acggaacaag
    29521 aagctgatag ctgatcatac aaccgacttg tgaacctttg ataatttcat gacaagcttt
    29581 tgtagctaaa gcacttgcta taaactgatg gtgtgcacct tggtatttcg cttgttccaa
    29641 atggggatgg ttttcttcaa ttactcctac gctaacaaaa gggtgatgtt ttacacagtt
    29701 gatctcattg aaagtcatcc aatattttac tttattttta tagcgtttga atactgtttc
    29761 agcgaaacga acgtaaaact gaataagctt tcgattaggc catccaccat attctgtaac
    29821 caaatataac ggcatttcat aatgggaaag agaaacgact ggctcaatcc catattttag
    29881 acattcgtca aacacttgat cataaaaaaa taatcccgct tcattgggtg tttcttcatc
    29941 accatgagga aaaattcttg tccatgcgat agacatccga tagcatttaa aacccatttc
    30001 agcaaataaa cggatgtctt ctttaaagtg atgatagaaa tcatttccgt gtcttttagg
    30061 ataataatac ttgctgttgg gatcctgcgc cgcttttact tgctgatgcg tcatcatatg
    30121 ccattgatct gtccattttt ctttaggtaa atcgctgtcg aatgtataca catcagacac
    30181 agataatcct ttttcatcta catcccatgc accttcacat tgattggccg ctgtcgctcc
    30241 gccccataaa aatccttctg gaaatttttt catatcgatc cccctaaact tgctagtttt
    30301 cttgtttgat tatttttctt atgactagta atgatatttt tgaatctact actatttgta
    30361 acaataacca ttgtggtcgg atcatatccc tctttgatta attcttgcaa atctacttcc
    30421 atcaataaat cgcctttacg tacttttttt ccatcagata ctgcactttg aaagtatttg
    30481 ccatttaaat tcactgtatc aataccgata tggatcagta tctctgctcc ttcttcactg
    30541 actaccccaa ttgcatgttt cgttggaaaa actgttgaaa cttccccgtt tacagaactg
    30601 tagatttttc cgtccgttgg aatgatcgca aacccttctc ctaaagatcc actagcaaat
    30661 gttggatcgt ttacttcaga taaatcaatt atttcacctg ctgcaggagt acttaacact
    30721 atatcctttg aggattcttc ttcgatttca tccttgtagc cgaaaagaaa agttagcata
    30781 caggaactga tcatcgctaa gaaataacta gcaactagcc aaacaaatcc ttcaccaata
    30841 taaatcggga aagacaaaat acttcttgta gcaactgcca aagcaccggc tcctgcggca
    30901 ccagctactc caccaaatat agctccacat atacaagcaa tgtaaaatgg tcgtttatat
    30961 ttaagagtca caccataaac agcaggttca gtgattccaa ataatgctgt gataaatgca
    31021 gataaagata aactttttac tttcttattt ttcgttttca aaaatacccc aagtactgct
    31081 cctgcttgcg cagctacggc agtcatacat gctggaccta gcgtatcacg accgtagaaa
    31141 gaaagattgt tggtcattac tggtacaagt ccccaatgta acccaaaaat cactaacact
    31201 tgccaaaatc cgccgataac agcaccagat aaaacagggc taaagttata aacagctaaa
    31261 tatccagctg caatcagctg acttgcataa tctgcaattg gacctacaac taataaaata
    31321 attgggacca tcacgatgat acaaatgaat ggtgtcaata aatttcgaat actttcgtgc
    31381 aagcttttgc gaatcttagg ctcaatataa gaaagaaccc atacagctaa aattgctgga
    31441 ataaccgtac ttgtatacct tgctagaatc acgggcatcc ctagaaatcg caaggttatg
    31501 ctatcactat atgcactgac aatcgtagga taaacgagag ctgctgcaat aaccatagcg
    31561 atgaaacgat ctgtattgaa cttttttgca gcagtatatg ccaaaaatat aggcaagaaa
    31621 tagaaaaatg catcagctgc tgcattcaaa atgatataag ttcctgattc agtagttaat
    31681 aaattcaaac tcgtacataa ggcgagtact cctttgatca aaccacttcc tgccaagacg
    31741 cctaaagtcg gggcaaaaac agcggcgatc atgtctataa aagagtttaa aatgcctttt
    31801 ttctcagttc ctctctttcc actagatgca tcatcttcta gtttcatttg tttggataat
    31861 acgtcataca cttcatttac tgcatttcct actacgactt gaaacattcc tccagcttcg
    31921 actacagtga tcacaccttc tagttgctct accttctcct tatctgcctt cttattatct
    31981 tttagggtaa agcgtaacct tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct
    32041 ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt ccatttttac ttcccccttg
    32101 ttaattaatg ccgtagcaat ccttcttaca agataagtat agttcttaga aaacggtttc
    32161 tccattcaat tagtacccct tctaatagcg aacgaaactg tacaatagtg tgtatggttt
    32221 acttagccga gcaaatcact cactgcttga aagaaaacaa gtatatcgtt tctcttatag
    32281 tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct gatgtataag tggtatctca
    32341 agctgatact ctgtgatttt tgtttctact tggcttttga taagacagca caacaaagtc
    32401 tcggcattta acggaagcat aactaggtca gaactaggag agattagttg ctgaatggaa
    32461 tctatctttt tatggatttt tatttgattt tcttctttgt agtaatgatc aagcagtttt
    32521 gttttctttt tttcttcaac tatggtcagc ttttcacgca aaatctgata aatctcccaa
    32581 gattcaaact catctagaac actaggtaga taaatcaaat catcccactc aaaaataagc
    32641 tcttgctcag tagtcaaaat tgcatcatat tccgttattt tgtttgattg atttttgaaa
    32701 agatacactg gttctatccg gcattctttg atccacatgt tcatactttg ttgaatctgt
    32761 ttttttagtg tattcaaaat actaattggc tggttactaa caatcagtat ctccccttgt
    32821 tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa
    32881 ttgggacgta attgaaaatg tattgggaag aaacgccgaa ttaaataaat ttctaaagga
    32941 taacgggaga tgatcttttg tacaaaatga ttcatggtat tatgtccggc atccatcctc
    33001 gttttcattt gtttgatatg ggttagcaaa aattctgttt catggaacaa taacgaaaca
    33061 ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc ttagattctt ctctatttct
    33121 ttgttttttt catcattaat catcaaataa tttaactcta atagtcgatt ttcaattgct
    33181 tgtttttctt
//
LOCUS       DANKAY010000021        31542 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000021 DANKAY010000000
VERSION     DANKAY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..807)
                     /locus_tag="PWY98_000980"
     CDS             complement(<1..807)
                     /locus_tag="PWY98_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2036746.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKK"
     gene            complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="PWY98_000981"
     CDS             complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="PWY98_000981"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2036747.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3308..3487)
                     /locus_tag="PWY98_000982"
     CDS             complement(3308..3487)
                     /locus_tag="PWY98_000982"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2036748.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3534..5357)
                     /gene="cadA"
                     /locus_tag="PWY98_000983"
     CDS             complement(3534..5357)
                     /gene="cadA"
                     /locus_tag="PWY98_000983"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2036749.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5635..7914
                     /locus_tag="PWY98_000984"
     CDS             5635..7914
                     /locus_tag="PWY98_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2036750.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8007..8654)
                     /locus_tag="PWY98_000985"
     CDS             complement(8007..8654)
                     /locus_tag="PWY98_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036751.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8833..9552)
                     /locus_tag="PWY98_000986"
     CDS             complement(8833..9552)
                     /locus_tag="PWY98_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2036752.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9683..10834)
                     /locus_tag="PWY98_000987"
     CDS             complement(9683..10834)
                     /locus_tag="PWY98_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2036753.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11051..11791)
                     /locus_tag="PWY98_000988"
     CDS             complement(11051..11791)
                     /locus_tag="PWY98_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2036754.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11795..12118)
                     /gene="rsfS"
                     /locus_tag="PWY98_000989"
     CDS             complement(11795..12118)
                     /gene="rsfS"
                     /locus_tag="PWY98_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2036755.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12175..12768)
                     /gene="yqeK"
                     /locus_tag="PWY98_000990"
     CDS             complement(12175..12768)
                     /gene="yqeK"
                     /locus_tag="PWY98_000990"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2036756.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12752..13402)
                     /locus_tag="PWY98_000991"
     CDS             complement(12752..13402)
                     /locus_tag="PWY98_000991"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2036757.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13412..13723)
                     /gene="yhbY"
                     /locus_tag="PWY98_000992"
     CDS             complement(13412..13723)
                     /gene="yhbY"
                     /locus_tag="PWY98_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2036758.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13739..14848)
                     /gene="yqeH"
                     /locus_tag="PWY98_000993"
     CDS             complement(13739..14848)
                     /gene="yqeH"
                     /locus_tag="PWY98_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2036759.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14845..15372)
                     /locus_tag="PWY98_000994"
     CDS             complement(14845..15372)
                     /locus_tag="PWY98_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2036760.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15552..16337)
                     /locus_tag="PWY98_000995"
     CDS             complement(15552..16337)
                     /locus_tag="PWY98_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2036761.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16330..17199)
                     /locus_tag="PWY98_000996"
     CDS             complement(16330..17199)
                     /locus_tag="PWY98_000996"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2036762.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17200..18579)
                     /locus_tag="PWY98_000997"
     CDS             complement(17200..18579)
                     /locus_tag="PWY98_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2036763.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18587..19006)
                     /gene="fabZ"
                     /locus_tag="PWY98_000998"
     CDS             complement(18587..19006)
                     /gene="fabZ"
                     /locus_tag="PWY98_000998"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2036764.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19031..19507)
                     /gene="accB"
                     /locus_tag="PWY98_000999"
     CDS             complement(19031..19507)
                     /gene="accB"
                     /locus_tag="PWY98_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2036765.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19510..20745)
                     /gene="fabF"
                     /locus_tag="PWY98_001000"
     CDS             complement(19510..20745)
                     /gene="fabF"
                     /locus_tag="PWY98_001000"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2036766.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20764..21501)
                     /gene="fabG"
                     /locus_tag="PWY98_001001"
     CDS             complement(20764..21501)
                     /gene="fabG"
                     /locus_tag="PWY98_001001"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2036767.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21513..22430)
                     /gene="fabD"
                     /locus_tag="PWY98_001002"
     CDS             complement(21513..22430)
                     /gene="fabD"
                     /locus_tag="PWY98_001002"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2036768.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22537..22761)
                     /locus_tag="PWY98_001003"
     CDS             complement(22537..22761)
                     /locus_tag="PWY98_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2036769.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22814..23776)
                     /locus_tag="PWY98_001004"
     CDS             complement(22814..23776)
                     /locus_tag="PWY98_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2036770.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23786..24226)
                     /locus_tag="PWY98_001005"
     CDS             complement(23786..24226)
                     /locus_tag="PWY98_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2036771.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24532..25497
                     /locus_tag="PWY98_001006"
     CDS             24532..25497
                     /locus_tag="PWY98_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2036772.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25554..25784)
                     /locus_tag="PWY98_001007"
     CDS             complement(25554..25784)
                     /locus_tag="PWY98_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2036773.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25870..26757)
                     /locus_tag="PWY98_001008"
     CDS             complement(25870..26757)
                     /locus_tag="PWY98_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2036774.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26846..28069)
                     /locus_tag="PWY98_001009"
     CDS             complement(26846..28069)
                     /locus_tag="PWY98_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2036775.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28086..29132)
                     /locus_tag="PWY98_001010"
     CDS             complement(28086..29132)
                     /locus_tag="PWY98_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2036776.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29296..31023)
                     /gene="ptsP"
                     /locus_tag="PWY98_001011"
     CDS             complement(29296..31023)
                     /gene="ptsP"
                     /locus_tag="PWY98_001011"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2036777.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31023..31289)
                     /locus_tag="PWY98_001012"
     CDS             complement(31023..31289)
                     /locus_tag="PWY98_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2036778.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
BASE COUNT         8694 a         6926 c         5431 g        10491 t
ORIGIN      
        1 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
       61 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
      121 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
      181 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
      241 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
      301 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
      361 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
      421 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
      481 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
      541 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
      601 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
      661 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
      721 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
      781 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
      841 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
      901 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
      961 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     1021 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     1081 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     1141 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     1201 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     1261 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     1321 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     1381 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     1441 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     1501 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     1561 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     1621 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     1681 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     1741 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     1801 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     1861 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     1921 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     1981 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     2041 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     2101 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     2161 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     2221 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     2281 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     2341 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     2401 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     2461 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     2521 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     2581 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     2641 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     2701 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     2761 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     2821 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     2881 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     2941 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     3001 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     3061 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     3121 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     3181 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     3241 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     3301 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     3361 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     3421 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     3481 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     3541 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     3601 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     3661 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     3721 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     3781 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     3841 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     3901 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     3961 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     4021 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     4081 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     4141 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     4201 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     4261 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     4321 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     4381 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     4441 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     4501 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     4561 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     4621 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     4681 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     4741 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     4801 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     4861 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     4921 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     4981 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     5041 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     5101 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     5161 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     5221 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     5281 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     5341 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     5401 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     5461 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     5521 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     5581 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     5641 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     5701 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     5761 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     5821 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     5881 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     5941 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     6001 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     6061 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     6121 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     6181 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     6241 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     6301 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     6361 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     6421 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     6481 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     6541 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     6601 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     6661 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     6721 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     6781 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     6841 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     6901 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     6961 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     7021 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     7081 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     7141 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     7201 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     7261 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     7321 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
     7381 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
     7441 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
     7501 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
     7561 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
     7621 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
     7681 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
     7741 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
     7801 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
     7861 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
     7921 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
     7981 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
     8041 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
     8101 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
     8161 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
     8221 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
     8281 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
     8341 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
     8401 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
     8461 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
     8521 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
     8581 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
     8641 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
     8701 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
     8761 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
     8821 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
     8881 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
     8941 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
     9001 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
     9061 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
     9121 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
     9181 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
     9241 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
     9301 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
     9361 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
     9421 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
     9481 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
     9541 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
     9601 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
     9661 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
     9721 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
     9781 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
     9841 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
     9901 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
     9961 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    10021 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    10081 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    10141 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    10201 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    10261 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    10321 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    10381 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    10441 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    10501 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    10561 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    10621 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    10681 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    10741 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    10801 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    10861 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    10921 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    10981 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    11041 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    11101 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    11161 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    11221 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    11281 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    11341 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    11401 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    11461 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    11521 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    11581 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    11641 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    11701 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    11761 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    11821 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    11881 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    11941 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    12001 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    12061 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    12121 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    12181 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    12241 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    12301 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    12361 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    12421 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    12481 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    12541 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    12601 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    12661 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    12721 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    12781 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    12841 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    12901 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    12961 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    13021 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    13081 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    13141 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    13201 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    13261 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    13321 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    13381 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    13441 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    13501 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    13561 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    13621 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    13681 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    13741 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    13801 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    13861 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    13921 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    13981 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    14041 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    14101 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    14161 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    14221 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    14281 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    14341 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    14401 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    14461 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    14521 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    14581 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    14641 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    14701 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    14761 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    14821 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    14881 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    14941 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    15001 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    15061 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    15121 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    15181 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    15241 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    15301 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    15361 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    15421 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    15481 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    15541 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    15601 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    15661 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    15721 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    15781 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    15841 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    15901 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    15961 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    16021 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    16081 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    16141 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    16201 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    16261 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    16321 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    16381 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    16441 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    16501 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    16561 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    16621 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    16681 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    16741 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    16801 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    16861 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    16921 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    16981 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    17041 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    17101 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    17161 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    17221 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    17281 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    17341 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    17401 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    17461 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    17521 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    17581 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    17641 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    17701 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    17761 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    17821 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    17881 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    17941 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    18001 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    18061 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    18121 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    18181 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    18241 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    18301 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    18361 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    18421 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    18481 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    18541 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    18601 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    18661 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    18721 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    18781 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    18841 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    18901 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    18961 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    19021 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    19081 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    19141 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    19201 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    19261 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    19321 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    19381 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    19441 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    19501 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    19561 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    19621 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    19681 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    19741 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    19801 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    19861 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    19921 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    19981 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    20041 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    20101 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    20161 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    20221 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    20281 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    20341 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    20401 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    20461 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    20521 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    20581 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    20641 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    20701 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    20761 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    20821 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    20881 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    20941 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    21001 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    21061 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    21121 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    21181 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    21241 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    21301 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    21361 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    21421 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    21481 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    21541 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    21601 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    21661 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    21721 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    21781 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    21841 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    21901 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    21961 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    22021 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    22081 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    22141 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    22201 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    22261 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    22321 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    22381 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    22441 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    22501 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    22561 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    22621 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    22681 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    22741 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    22801 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    22861 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    22921 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    22981 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    23041 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    23101 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    23161 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    23221 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    23281 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    23341 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    23401 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    23461 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    23521 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    23581 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    23641 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    23701 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    23761 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    23821 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    23881 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    23941 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    24001 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    24061 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    24121 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    24181 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    24241 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    24301 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    24361 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    24421 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    24481 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    24541 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    24601 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    24661 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    24721 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    24781 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    24841 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    24901 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    24961 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    25021 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    25081 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    25141 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    25201 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    25261 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    25321 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    25381 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    25441 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    25501 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    25561 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    25621 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    25681 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    25741 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    25801 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    25861 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    25921 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    25981 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    26041 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    26101 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    26161 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    26221 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    26281 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    26341 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    26401 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    26461 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    26521 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    26581 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    26641 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    26701 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    26761 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    26821 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    26881 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    26941 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    27001 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    27061 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    27121 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    27181 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    27241 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    27301 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    27361 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    27421 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    27481 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    27541 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    27601 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    27661 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    27721 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    27781 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    27841 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    27901 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    27961 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    28021 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    28081 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    28141 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    28201 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    28261 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    28321 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    28381 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    28441 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    28501 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    28561 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
    28621 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    28681 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    28741 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    28801 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    28861 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    28921 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    28981 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    29041 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    29101 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    29161 aacatagcct acaaactgtc actcaaactt aagccctatt tttgtctatt cttactgaca
    29221 aaaacgtttt cttaaaaaag aacaaaaaaa ctggcgtagc tcttttacaa gccacaccag
    29281 cttttgatac gttgtttatt ttacatattc atgaacaagt tcaacgactt cttccatcgt
    29341 atcgcattct ttcaatgcac gatcagcaag ttcagccatt ttcgctgtgt ccaagcgttt
    29401 cattaaacta cgcgttttaa gaatagatgt cgcactcatt gagaactcat ctagacccat
    29461 acctactagc aatggaacag ctgtttgatc tccagccatt tctccacaca tacctgccca
    29521 ttttccttca gcgtgtgctg catcaattac atttttgatc aaacgtaaaa ttgatgggtt
    29581 atatggttgg tacaagtaag aaacgcgttc gttcatacgg tctgctgcca ttgtgtattg
    29641 aatcaaatcg ttcgtaccta cagagaagaa atctacttct tttgcaaact tgtctgctaa
    29701 gactgctgct gctggaatct cgatcatgat gccgacttgg atcgtatcag atacttcctt
    29761 gccttcactt atcaattttt gtttttcttc ttcaaagatc gcttttgctg cgcggaattc
    29821 tttgagtgta gctaccatag ggaacatgat acgtaaatta ccatgaacag atgcacgaag
    29881 caatgcacgc atttgtgtac ggaacatttc gtcgccttgt tcagataaac tgatacgcaa
    29941 tgcacgatat cctaagaatg gattcatttc atgaggtaat tgcagatatg gtagttcttt
    30001 atctccaccg atgtccattg tacgaacaac tacaggtttt ccttccatac cttctaatac
    30061 agctttgtaa gcttcatatt ggtcatcttc tgttgggaag tctggagaat ccatatacag
    30121 gaattcagta cggtacagac caactgcttc tccaccatta ttatggacac caactagatc
    30181 ttttggtgtt ccgatgtttg cagctaattc aaaatgcttg ccgtcagctg tgacagtttc
    30241 tgcatttttt aatttttccc attctgcttt ttgtgctgcg taatctgcac ctgctttttc
    30301 aaattcagct ttttgttcat ctgtaggatc gatgattaca tcgccttcga tcccgttgac
    30361 agccaaaata tcgccttctt tgactttagc agtaatttct tttgtcccaa cgattgctgg
    30421 aatttctaaa gaacgtgcca tgatagcaga atgtgaagta cgtccaccaa tgtctgttac
    30481 aaacgctttt acaaaattgc gatctaattg tgctgtatca cttggagtca agtcatgtgc
    30541 aacaacgacc acttcttcgt tgatcattga tggatttggt aaagtaacgc ctaataagtg
    30601 cgctaaaata cgtttagcaa cgtcacgaat atctgcagca cgctcttgca tataagcatt
    30661 atcttccatt gcttcgaaca taccgatata catatccgtt acttctttta gcgctgattc
    30721 agcatttacg ctgttgtctt taatattttg cttgatttga cctaccattt ctggatctga
    30781 aagaaccatc aaatgggcat caaatacttg cgcttctgct tcacctaagc tttgcgctgc
    30841 tttttcacga atttgctgca attcttcagt agatttagct aatgcagcat ccaaacgagc
    30901 ttcttcagcc gcagtatctt ctactgttgc cttactgaat gttaaatccg gttgaactag
    30961 caggtaagct tttgcaacgg ctacaccgtc actagcggca atccctttta gcatttcaac
    31021 cattattcag ataagccttc tttcttcatt gtttcaacga ttgctgccat tgcatcagct
    31081 tcgtcagcac cttctgcagt aatagtaaca tcagaacctt gacctacacc taaagacata
    31141 acgcccatga ttgatttcaa gtttactgat ttacctttat actctaagtt tacatcagag
    31201 ttaaatttgc ttgctgtttg tactaatagt gtagctggac gagcgtggat cccagtttct
    31261 gctactacgt gaaattcttt cttttccata ttaatcggtc tcctttaagt gagtgttgtt
    31321 tttatgtgag ggttttcttt tttagtaatt cagagaatcc taacaaatgc caccctttca
    31381 tctgtaaaga ttaacatttt ttttcgttag atacaacagt tttcattcaa tttcagataa
    31441 aagtattcaa attattccgg attctttttc atccttttct tcacatttta taacaaacgt
    31501 gtttatttcc tttttcttgt cgctttctct tctcctgacg tt
//
LOCUS       DANKAY010000022        30971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000022 DANKAY010000000
VERSION     DANKAY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(130..1005)
                     /locus_tag="PWY98_001013"
     CDS             complement(130..1005)
                     /locus_tag="PWY98_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2036779.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1114..2883)
                     /gene="aspS"
                     /locus_tag="PWY98_001014"
     CDS             complement(1114..2883)
                     /gene="aspS"
                     /locus_tag="PWY98_001014"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2036780.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2898..4190)
                     /locus_tag="PWY98_001015"
     CDS             complement(2898..4190)
                     /locus_tag="PWY98_001015"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2036781.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4584..5531
                     /locus_tag="PWY98_001016"
     CDS             4584..5531
                     /locus_tag="PWY98_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2036782.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5620..6879)
                     /locus_tag="PWY98_001017"
     CDS             complement(5620..6879)
                     /locus_tag="PWY98_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2036783.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7050..7496)
                     /locus_tag="PWY98_001018"
     CDS             complement(7050..7496)
                     /locus_tag="PWY98_001018"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2036784.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7507..9720)
                     /locus_tag="PWY98_001019"
     CDS             complement(7507..9720)
                     /locus_tag="PWY98_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2036785.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9953..10717)
                     /locus_tag="PWY98_001020"
     CDS             complement(9953..10717)
                     /locus_tag="PWY98_001020"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2036786.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10718..11665)
                     /gene="prmA"
                     /locus_tag="PWY98_001021"
     CDS             complement(10718..11665)
                     /gene="prmA"
                     /locus_tag="PWY98_001021"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2036787.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11678..12166)
                     /locus_tag="PWY98_001022"
     CDS             complement(11678..12166)
                     /locus_tag="PWY98_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2036788.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12132..12803)
                     /locus_tag="PWY98_001023"
     CDS             complement(12132..12803)
                     /locus_tag="PWY98_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2036789.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12941..14227
                     /locus_tag="PWY98_001024"
     CDS             12941..14227
                     /locus_tag="PWY98_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2036790.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14299..14493
                     /gene="ssrS"
                     /locus_tag="PWY98_001025"
     ncRNA           14299..14493
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWY98_001025"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14571..14855
                     /locus_tag="PWY98_001026"
     CDS             14571..14855
                     /locus_tag="PWY98_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036791.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14928..15407
                     /locus_tag="PWY98_001027"
     CDS             14928..15407
                     /locus_tag="PWY98_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2036792.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15630..15773
                     /locus_tag="PWY98_001028"
     CDS             15630..15773
                     /locus_tag="PWY98_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2036793.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16351..17934
                     /locus_tag="PWY98_001029"
     CDS             16351..17934
                     /locus_tag="PWY98_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2036794.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18371..19555)
                     /locus_tag="PWY98_001030"
     CDS             complement(18371..19555)
                     /locus_tag="PWY98_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2036795.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19580..20587)
                     /locus_tag="PWY98_001031"
     CDS             complement(19580..20587)
                     /locus_tag="PWY98_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2036796.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20759..21118)
                     /locus_tag="PWY98_001032"
     CDS             complement(20759..21118)
                     /locus_tag="PWY98_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036797.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21090..21461)
                     /locus_tag="PWY98_001033"
     CDS             complement(21090..21461)
                     /locus_tag="PWY98_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2036798.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21478..21780)
                     /locus_tag="PWY98_001034"
     CDS             complement(21478..21780)
                     /locus_tag="PWY98_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036799.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21797..22267)
                     /locus_tag="PWY98_001035"
     CDS             complement(21797..22267)
                     /locus_tag="PWY98_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2036800.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22242..22520)
                     /locus_tag="PWY98_001036"
     CDS             complement(22242..22520)
                     /locus_tag="PWY98_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2036801.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22517..23566)
                     /locus_tag="PWY98_001037"
     CDS             complement(22517..23566)
                     /locus_tag="PWY98_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2036802.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23523..24452)
                     /gene="tadA"
                     /locus_tag="PWY98_001038"
     CDS             complement(23523..24452)
                     /gene="tadA"
                     /locus_tag="PWY98_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2036803.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24584..26338
                     /gene="ade"
                     /locus_tag="PWY98_001039"
     CDS             24584..26338
                     /gene="ade"
                     /locus_tag="PWY98_001039"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2036804.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26436..27098)
                     /gene="trmB"
                     /locus_tag="PWY98_001040"
     CDS             complement(26436..27098)
                     /gene="trmB"
                     /locus_tag="PWY98_001040"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2036805.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27168..27950)
                     /locus_tag="PWY98_001041"
     CDS             complement(27168..27950)
                     /locus_tag="PWY98_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2036806.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28154..29365)
                     /locus_tag="PWY98_001042"
     CDS             complement(28154..29365)
                     /locus_tag="PWY98_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036807.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29358..30095)
                     /locus_tag="PWY98_001043"
     CDS             complement(29358..30095)
                     /locus_tag="PWY98_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036808.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30233..30667
                     /locus_tag="PWY98_001044"
     CDS             30233..30667
                     /locus_tag="PWY98_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2036809.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30660..>30971
                     /locus_tag="PWY98_001045"
     CDS             30660..>30971
                     /locus_tag="PWY98_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2036810.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LK"
BASE COUNT         9132 a         6557 c         5526 g         9756 t
ORIGIN      
        1 tttcaacttt aagaaaattt caaataaaaa agcagttttt ctttgctgat tctactatgg
       61 tagaaaaaaa ctgcagacaa acgaacgtct gtctacagca gtatttcatg aatttttgtc
      121 aaactcgatc taaatgccat cctcatagaa acgaccaagt atctggacgt tttctgcaat
      181 acttacaaaa gcttgtggat cagaagcttt cattgcttct ctaagtagag gcagttcgta
      241 tcgtgtaacg atcgtcatca atactgtctg cttatcatgc ttataagcac cttctgcttc
      301 attgatgatc gtgatccctc tacgcataca gttttggatt tcatttacta ctttttctgg
      361 atgtttcgtt acgatcatta cctgcatctt cttttgttta gtataaacga catcagttac
      421 ttttccactg acgaaaatag acaacgtact gtaaaacata tattgccagc caaatagaat
      481 tccggcaaca aaaacgatca atgcattgaa gtagatcgaa atagaaccaa tcgactttcc
      541 tgttttttta cgaatcgtga tactcacgat atctagaccg cccgatgata ggccgtttct
      601 caacgctagc ccaataccta gtcccatcac agccccccca aacaacgcac agatgattgg
      661 atcggtagat aggactgttt ccggtaaaat atggatacaa agggaggtca aagtgactgt
      721 gataaaagtg aaaattgtaa acttcttccc gatttttaac cacgccaata caaacaaagg
      781 tatattcagt aaatacaaag ttgttgatac cggtacttcg aatcctatca gtgattttga
      841 caaagtcgtc aaaatctgag ctagaccagt aataccactt gaataaatat gtcctggctg
      901 ataaaagaaa ttcaatgcga ctgatgcgat caacgcataa acgatagata ccgaaagctt
      961 ggtcgtgtaa tcatgataag cccaattttc gaatgttttt ttcatggaaa agccccgctt
     1021 tatatgttat ggcttcatta taaagtgttt ttcaattaaa tcaaggttgt caaaagaagc
     1081 gtattcgaca aatccttttt acctttttat cggctattct tctatattcg ttacatcaat
     1141 attcaattcg aacaattgta aaggcgaaac catacttggt gcatcactca tcacatctgc
     1201 tgcttttccg tttttaggaa aggcaatgac ttcacgaata ttatcttctc cggctaataa
     1261 cattgcaaaa cggtccaatc ctaatgcaat accgccatgt ggagggaatc cgtaatctaa
     1321 agctgttaac aagaaaccaa attgttcttc catttgttct tttgtaaagc ctagagtttc
     1381 tagtactttt tcttgaagct cacgagtatg aatacggata gaacctccgc ctaattcata
     1441 accgttcaag acgatatcat aagcttctgc atagactttt gcagggtctt cagccaataa
     1501 cgggatatct tcttcttttg gcatcgtgaa tggatggtga gcagagacat atcgaccttc
     1561 ttcttctgaa aattcaaatt gtggccaatc agttacccat aagaaattga atttcgattc
     1621 atcaattagt cctaattctt ttcctaaacg tgtccggata gcacctaatg cagcagcgac
     1681 gatttcactt ttatctgcgc caaacatcag caagtcacct ggttttgcat ctgttgcttt
     1741 aatgattgct tctgttgctt ctcccatgaa tttagcaatt ggacctttca atccgtcttc
     1801 ttctactttc agccatgcaa gccccttagc tccgaattgt cctacatatt gacctaactg
     1861 atccatgtct tttcgtgagt agcgatctgc agcgccttta gcattcaatg ctttcacgac
     1921 accgccgttt tcaagagcca tttggaagac tttgaattct acttctttga ctgtatcgct
     1981 tagatcgatc aattccatat caaaacgtgt atccggtttg tcgctgccgt agcgagccat
     2041 tgcttcatca taggtcatac gaggaaatgg caatgtgact tcgataccgc gaacttcttt
     2101 cattaccttc gcaatcaatc cctctgtgta agtttggatt tcttctgctg ttaagaacgt
     2161 tgtttcaata tctacttgcg taaattcagg ctgacggtct ccacgtaaat cttcatcgcg
     2221 gaaacaacga acgatttgat aataacggtc gaatccagca ttcatcagca attgtttaaa
     2281 caattgagga gattgcggca acgcatagaa atgtccagca tggacacgtg aaggaactag
     2341 gtagtctctt gcgccttctg gtgtagattt ccctaggtaa ggtgtttcga tatcgagaaa
     2401 gtcattatca tctaaataat gacgaatcgc ttttgttgtc gcatttctta atttgatgtt
     2461 tttagtcatt tttggacgac gcagatccaa ataacgatat ttcaaacgta tttcgtcact
     2521 gattgcttga tcatcttcaa tttgaaatgg cggggttttt gcttgattca aaatcacgat
     2581 ttcacttgca atcacttcga attcaccagt ggccatttta ggatttactg cattttctcc
     2641 gcgtcttcta acttccccag tcacttcgat cacgtattca ctgcgacatt tatccgcgat
     2701 ttcccatgct tctttgttga tttcaggatt aaagacgatt tgaacgattc cttcacgatc
     2761 acgtaggtcg ataaagatta ctcccccaag gtctcgtctt ttttgtaccc atccttttaa
     2821 tgtaactgtt tgttctaatt gttgttcgga aactaatccg caatagatcg ttctttgtgc
     2881 catttatttt tttcctccta ctcgaataaa gtcatttcat catagacttc atcaaaatgt
     2941 tcatatatct cttctaatgg aaaacttttt tcttttcggc ttgccattgc tttgacatta
     3001 atcgtttgac tagcaagctc atcttcaccg atcaccaata ctaatttggc attcgcttta
     3061 tctgctgtct taaattgcgc ttttgcttta cggttcatga agtcgcggtc agctgaaaaa
     3121 ccaaagtttc gaatcgcttg gaccactttc aatgcttcga tatttgtttc gtctccgagt
     3181 gcgaccacgt aagcatccaa ctcattaaat gcagggataa ctacttcctc tgcctccata
     3241 gtcaagagta atcgctcgat acccattgca aatccgaagc caggtgttga aggaccacct
     3301 agttcttcca ctagattgtc ataacgtccg cctgcgcaaa tcgttgcttg tgcccccatt
     3361 tttggtgcgt cactcatgat ttcaaaaatc gtatgtgtgt aataatctag tccgcgaacc
     3421 atattactgt cgatttcata aggaacagcc aatgcatcca gcatggaaac aacggtatca
     3481 aaatgttttt gagcaggctc gcttagataa tctaagatag atggagcttc tgccacaaat
     3541 ttttgatctc gtttgtcttt actgtccaat acgcgtaatg gattttcatg aaggcggcgt
     3601 ttagagtctt cactcagctc tgattcaaaa ggtaaaagat agtcgatcaa tgcttgacga
     3661 taggcttgac gcgtttcttt atcacctaat gaattgatga ctagccggat ttgcgtgatc
     3721 cccaactgtt tgaagaaatc aagtgccatc accatgctct ccacatcagt tgcagggttt
     3781 tctgatccaa atgcttctac tccgatttga tggaattgac gtaaccgtcc tttttgaggg
     3841 cgttcgtagc gaaacattgg tcccatataa aaagttttga acggtttcgc atactctggg
     3901 ccgaacaatt tatgctcaac aaaagaacga acgattggtg ctgtaccttc tggtcgaagc
     3961 gtcacatggc gttccccttt gtcataaaaa tcatacatct cttttgaaac gatatccgtt
     4021 gtatccccta cacttcggga tatcacttca tagtgttcaa aaatcggtgt acgcatttca
     4081 ttatactgat aatctctgaa taataaacgt gctgtttctt cgacaaactg ccatttttct
     4141 gattcgcctg gcaaaatatc atttgttcct tttggtcttt ggaaactcat agttttctct
     4201 ccctttctta gctatgtaaa taaaaaaatc acccttgttc atcatgaaca agggtgaatg
     4261 aaattcatac acggtaccac ctaattttga aagatttctc tttcctctta cgaataacgc
     4321 tcgtacgcgg gacggctttt cactcgtcca tctccagagt gtctttaggc taatgaaact
     4381 ttagatcatt ttcagcctcg atgatcctct cttgtaaagt ttcaaaaact tactcgctct
     4441 ttcattgaca ttcgatatta cttataacct actaaaaacc agtctcaaag tcaagagata
     4501 tctgactatt tcccctcaca aaaacgtttt agttcagctt tttggtattt tgttttagac
     4561 tagacaaaaa ggaggaatca gctatggcag aaataagaat cggacattca caagtctacg
     4621 cagaacagct tggattagga gcaaatgccg ttggaggaca taatcttttt gacggtttgg
     4681 aagatgagac aggaaaacaa gtagttcgta cagctttgaa tagcgggatc aatttgatcg
     4741 ataccgctta tgcttacgga aacggccgtt ccgaagaact gattggcgag gtactgaagg
     4801 aaaaagaata tgatcgctca cgtgtcgtca tcgctacaaa agctgctcat gtaccaaata
     4861 aagggcgaac atttgataat tctcctgagt ttctcaaaca atcggtggaa gatgcactaa
     4921 aacgtctgca aactgattac atcgacattt tttatatcca ctttccggat gaaagcacac
     4981 caaaaaatga atcagttgct acattgcacg aattaaaaga agcaggaaaa atccgagcag
     5041 tcggtgtgtc aaacttcacg ttagaacaat tgaaagaagc gaatgcagat ggatatgtcg
     5101 atgtagtaga agataaatac agtttgatcc atcggcaggc tgaaaaagaa ttattccctt
     5161 atcttgaaaa aaacaaaatc agttttgttc cctattttcc tcttgcttcc ggtttgctga
     5221 caggaaaata tgaacttggt gaggaaaaac aatttggtga aggagatcct cgaaaaagaa
     5281 atccagattt ccaaggagaa cgtttcagag aaattttaac cgctgtcgat gtacttcgcc
     5341 ccattgctaa aagataccaa gccactcctg cgcaacttgt tttagcttgg tacatgaaga
     5401 atccacgagt gtctgtagtt atacctggag ctaagcgtcc agagcaagta tctgacaatg
     5461 tccaagcttt agatttgcat ttatccaatg aagattatca aaccattgat gaagcatttc
     5521 gtggattttg atcaattgac cttatcaatt tatagtaata tgaaaaaagg aagatcagca
     5581 ctgtttgatc ttcctttttt tcgataagtt tatgctaatc taattcttgt aaagtagcgc
     5641 tcggctattc ggataagtcg gaaactgatt gaatggaatc gtccaatctt gttcaggtac
     5701 agagaactca aacttattta ctagctgatc ggaaaacaca cgtagactat gaagagtgat
     5761 ccattctcct gcacagcgat gcatttgtcg aaaatttccc ccgccttgtg caatcatctc
     5821 atattcttct ttataggaaa tatcttttgc tttccctaca taccgtttga tcataaagga
     5881 atctggcgct tcaactgttc gttcatcatg gtctgttcca tataaatcta aaataaccca
     5941 actgccttct ggtatacgat aaccatctac ttctacttct ttcagcgaaa tcgcaggtag
     6001 cattgggaag aaaggataat agcgtctcat ttcttgaata aacggatctt gtaacgtcga
     6061 aaagtcattt tttaattgct gatacaaatc tggtctactg aaaagtgcat gtcccatcaa
     6121 tgccgcccac acagttagag caaccgttgg acggatgatg ttcaataatt caacagctgc
     6181 cacttccaga ggtaaaagct gaccatctaa atctgtggca ttggcaaaag catataatgc
     6241 cacattttct tttccagcca caggattttg tcgagcttct tcaatcaagc cttgcgccca
     6301 tttttctgat tttttgcgat tttcgacacc ttttatgtga tcaatcggag aagtaaacgt
     6361 tccgctgatc atagagattt gatattctgc taatttttcg acctcatctt tagaaatcgc
     6421 tgataaattg atacctgccc actcgcaaat agatgtaaag aaaaccatct tacttaaatc
     6481 aaatagttca aattgaccat gctgtgcttc aagtgcttgt gtcagattct tatctaagat
     6541 acgatgataa tcttccattc tttccggtgt cattagatcc ataaaaatat ttttccgatg
     6601 gtgatgagca gctccatcca aagtctgtac tccaccttga ccaaaaagtg tcttcaatac
     6661 taattttggc atagcacctt ttcgtttgaa attccttgga tcataaaata cttttgctgc
     6721 tgatgatccg tagatcgtga tagcttcttt attgaaaatc tttgctttga cgacgggagc
     6781 gtccgcttca tggcgtaact cctccaacat gttgtatcct ttttgataca atttttttaa
     6841 atctgtgatt ttgatatcta ctactggtac ttctttcatc ttcagcaacc tcctataaaa
     6901 aataacttgt ataatttaag cttaactttt tttccgactg ctgacaaaag aagcgctttt
     6961 tgcagacaaa aaagccactt aagttagagt ttatcagtct aacttaagcg acgtgtttca
     7021 tagtttttac tagtgaaaac cagattactt tatttgtttt tagaatcaat aatgatcgtt
     7081 acaggtccat cattgacgag agaaaccgac atatctgctc cgaattgtcc ggtagcgact
     7141 gtgatgccgc gacttctgat tccttcattg aatgattcgt ataaaggaat agctgtttct
     7201 ggtcgtgcgg cttccacaaa gcttggtcga tttccttttt tcgtatccgc aaacagtgtg
     7261 aattgagaga tacttaaaat ttgtcctccc acatcttcta cactaatatt catttttcct
     7321 tgttcatctt caaaaatacg catttgtgca atttttttta ccagatagtc cacatctttt
     7381 tgcgtatcgg tttcatgaat gcctaataaa acaagtaggc cgtagccaat ttttccaatt
     7441 tcttgctgat cgactgacac agcagcttta cttacccgtt gaacaactgc tctcatatct
     7501 gtcctcctat ccattcgtcc ggcgtacact gtagacatca ggtactgctt tgattttatc
     7561 cacgatactc tttaggtggg ataaattctg gataccaacc gtcaaatgga tcgtcgccat
     7621 tttatcttta ttcgtccgtg cttcaacgct gttgagattt ttcgttagcg cattgactgt
     7681 ttgaagtaca tcattcaata agcctgaacg attatagccg taaatttcca aatctgcatc
     7741 atactctttt cgtgtattcg atgtatcttc ccattcgact tcaatcaaac gttctgctac
     7801 atttggtttg tcaggctgaa cattcggaca atctcgacga tgaatggata tccctctgcc
     7861 tttagtgata taaccaacga tatcatcacc aggaatcgga ttacagcagc gactgatacg
     7921 aattagtaag ttttctaccc cttgaatgac aacgccacct tcatgacgta ctttcatttt
     7981 gtcaggttct tttttcggct gattcatgat ttcttctgct tcttgctttt gctgctcgat
     8041 tttttgttct ttacgttctt tttcagtcag acgattggcc atcgtcaagg ggctaacttc
     8101 tccataccct acagctgcat agagatcatc ttctgtttga taattaaaac gatcgagtgc
     8161 ttcttgcagt ttgttcttcg tcaaaatatc tttaggcgta aatcctagat ctgtaataca
     8221 tttgaccacg gattcgtggc ctttgatcac attttcttct cgatcttggg ctttgaagaa
     8281 acgtttgatc ttatttcttg ctttgctagt agcaactaat ttcaaccagt cgcgacttgg
     8341 gccaaatgaa tttggagaag tcatgatctc aatgatatct ccgtttttca atttgtaatc
     8401 aagctgcacc attttgccat ttacttttgc accagtggtt ttgttaccga tatctgtatg
     8461 gatactgtat gcaaagtcca atggtccgga tccttttggt aactcagtaa cgtctccttt
     8521 tggtgtgaag acgtagactt tatcactaaa gatatctcct tttacgcctt ccataaattc
     8581 agaagcatca tagctttcgt cttgaagttc gagtatctca tggaaccagc ttaattgttt
     8641 cgtcatacca tctggttcta ctttttcatt ttttccttct ttataggccc agtgtgcagc
     8701 aaccccgaat tcagcgattt catgcatttc ctgtgttcgg atttgaattt ctaccggatt
     8761 tcctgccgga ccgatcacgg tagtatgcaa agattggtac atattagctt ttggcatggc
     8821 aatataatcc ttaaatctgc cgggcattgg tttccatttt gtgtggattg ctcctaatac
     8881 agcataacaa tccttgattg aatctacgat cactcgaata gctagcaagt cataaatttc
     8941 gttgaactgc tttttctgat ctttcatttt acgatagatt gaataaatgt gttttggccg
     9001 tccatagatt tctgcaaaaa tccccagctc ttctgtcgct attcgaatat cttcaacagt
     9061 accacttacg tatttttcac gttcttctct cttcgtctgc atcaagtgga cgatacggta
     9121 atactgtttt ggatttagat aacgaagtgc tgtatcttct aattcccatt tgatccggct
     9181 gatccctaga cgatgtgcaa gaggcgcata gatttctaaa gtttcctgag cgatccttct
     9241 ttgcttatct tctcgcaggt gcttcaacgt acgcatgtta tgtagtctat cagctaattt
     9301 gaccatgata actcgtaaat cttgagccat tgctagcagc atctttcggt ggttttctgc
     9361 tagctgctct tcgtgggatt tatattttat tttccctaat ttggttacgc cgtccactaa
     9421 catagcaacg tcatcgccaa attcttctct cagatcttct aaagtgattt ctgtatcttc
     9481 aacaacatca tgtaagaagc ctgtagccac tgtatgggga tccattttta aatctgctaa
     9541 tatacctgcg acttggatag gatgaataat gtagggttcg cctgattttc taaactgtcc
     9601 gtcatgtgca gcagtcgcgt attcgcatgc tttctcaaca aatgctacat gttcgggacc
     9661 catgtattga ctaacgagct ttatgacacc gggtcccgtt aaaatttctt cttttgtcac
     9721 aggaatcgat ccccctttct tttttcggta aaacgggaat cgggacaaat atctgtttga
     9781 taattgtccc aaaatccgtg cagttatacg tttttttacg gaaatgttcg ctttttaaaa
     9841 gctttttcaa acgatttttc gtatccattg tataaataat tgtttctatt atacaaattg
     9901 ttgactattt tttcaattct ttctgtcgat actcttttat taatgaagaa cgttagctga
     9961 aagaaggatt cattaattcc cattgatagc tagcagcact taataaataa agaggtgctg
    10021 tttctgtacg aaggattctt ggtcctaatc cacaaagtat ccctttgtgt tctacaaaat
    10081 aatcgacttc atcgcgggat aatccacctt ctggtccaaa gacagctaag attcgactat
    10141 ctggttttgt ttcactaaga attttggctg catttgattt ttcgccttgc tttgctgatt
    10201 cctcataagc aaccaagacc acgtcataat catctagccg ctcaaataaa tcttgtttcg
    10261 tttccaataa ctctactgat ggcacgatct gccgatggga ttgttccgct gcctcttgag
    10321 caatcttttc aagccgttgt tgtcgtttca tacgtttttt tgcatcccat ttaacgacag
    10381 aggctgatgc cgggaaacca agaaaagctt gcgctcctaa ttcagtggat ttctgaacga
    10441 tccaatccag cttttcccct ttaggatagc cactcgctat ggtaatcgaa cttggcagtt
    10501 ccttttgatt ttcttctttt ttaatttctt tcagataaac agcctcttca gtaatctccg
    10561 tgatttctgc aacaatggct atttgatcat tgaatgccaa aaaaacttca tcctcgggtt
    10621 ccatcctcat aacccgtaca atgtggtgat agttttcccc ttctaaaaca aaatagtctt
    10681 ttgattcata tgattctttt aaaaaatacc gctgcatcta ttccacctct ggttttttca
    10741 aaataattgc ataccagtct ttttgctgga aaatttgatc cacgataaac ccttgttcta
    10801 acatggcatc cagaaccatt tgcttcttct cttcaatgat tcctgaaacg atcagcgtgc
    10861 ctgtttgctt cagtaaacgc caagcatctg ggatcatcaa taagatgata tctgctaaaa
    10921 tgttcgcaac gattacatca ctttcaatct cgattcctgt caaaagatcg ttagctgata
    10981 catggacatc tttggctatt tcattcatat ccatattttc tttcgccgaa cgaacggcta
    11041 cttcgtctaa atcaaaggca tagacatctt ttgcaccaag atattttgct gcaatactta
    11101 gcacgcccga cccagttccc acatctagca atgtttcacc accacgcaag actgtttcta
    11161 gtgctgtcaa tgtcaaggaa gtcgtgggat gagtgcctgt gccaaatgcc ataccaggat
    11221 ctagtgtgat aattttttct agaggatctt ctgtctggta gttttcccag cttggcgcaa
    11281 ccgtcagcat tcgcgtaata cgcaccggat gatagtattt cttccaggct gttgcccagt
    11341 tgctttcctc tacttcacta actgttactt tattttttcc aatagctaga ccaaactcag
    11401 gtaattgtgt gatacgatct ttgataaaag gcaagatttc cggtaagaaa gtcgtttccg
    11461 gaaaataagc catgactagt gcgccttctt tcagcgatgt aaaatcattt ttatctaaaa
    11521 tctcaccata ttgatccgac tgaaaatgtt caacatctaa agaatcttca atcgcgacac
    11581 cactcgcccc tgcctccatc aaaatattag caactgcttc gactgcttca ctttcagttt
    11641 ctacttttac ttcattccat ttcatatctg tcactcctta ataacttggg tacggtaaat
    11701 aagtatctgc ttcattttgc tgttttactt ctactaattc tgctagttgc tgattatccc
    11761 agtgaagctc gaatacagct tcgtcatcgt cttcatccaa aaaatcaagc aaatcgcttt
    11821 gcccttgagc taatacttca tttagatatt ccactaaaga atcagctact gattttgcca
    11881 accctttttt tccttcataa gggattactg ctagataatt gcgttcatca aaaacagatt
    11941 tctgaggatt aaaaagaagt acgccgtctt caaattcgat aattggttct tgcgaaacga
    12001 tcccttctgc atcatcaatc tcaaattgtg ctttattttc tgcaaacaag cgaacaacga
    12061 tctcgatcgt atgatttttt gtatcccaat ctaaagctac atcatagtct gtcacttgtt
    12121 tttcaattat ttcatctaaa tatgttaata gatccttctt tttcattttc tttcctccaa
    12181 ccccaatctt tctcgatttg gttttttctt tgtaaagaga tataaggatt tcccgacaca
    12241 acaaaacgca acggcttttc tgtccataca cctttgtttg ggataccgat tctaggcaaa
    12301 gcattgatct tttttggtgt cttttttttc tcaaaaacta agcgtaacga actatctgct
    12361 atagactgac cataaagttc ttgaggaatt gctaatgctt ccactaattt tcccggaccg
    12421 ttgctgatat ctggaccagt ttttccgcca cgatttttac tcatttgatc aatcattgcg
    12481 gcgggttcga ttgcacgtat cattactcct tgaggaatcc cttctggctg agtaatgatg
    12541 ttcaaaatcc gatgagtatg catcgtgtaa aggtagatcg tacctggttt ttcatacatt
    12601 gcccgcacac gcggtgttct tcttaaacca tagctatggg cggcctcgtc ttctggacct
    12661 aggtatgctt ccgcgtctac aatatatcca gctaatcttc cttctggtgt gtcatgctcc
    12721 aaatacatcc ccaataaatc ccgtgccact tctggcgtcg ttttattcca aaaaaattca
    12781 tctatctttt gtttatccgt catttttctc acctacttta tcatacacaa tattagttgg
    12841 ataagctatg aaaacaaaac aacagaaggc tgttctttct aggcatcttg ctaaatatga
    12901 tataattaag ggacgtaaaa acaagaagga ggaaaatcct atgcaacagc cattggctta
    12961 tcgcatgaga cctcgaacga tcgatgaagt cgttggtcag cagcatctag tcggggaagg
    13021 aaaaatcata cgccgcatgg tcgatgctcg catgctctct tccatgattc tatatggacc
    13081 tcctggtact ggaaaaacga gtatcgcaag tgcgatcgcc ggctctacta attatgcatt
    13141 tcgcatgcta aacgccgcta cagattcaaa aaaggatcta caagttgtag cggaagaagc
    13201 aaaaatgagt ggtacagtga tcctcttatt ggatgaagta caccgattag ataaaacaaa
    13261 acaggatttt ctgcttcctc atttagaaag tggccgaatc atcttgatcg gagcgactac
    13321 tgagaatcct tatattacca tcaatccagc gatccgcagc cgtacacaga tctttgaagt
    13381 caaaccatta acagaacaag acatccagtt ggcagtcgaa catgctttaa gggataaaga
    13441 acgcggcctt ggacaacaag caatccagtt agacgaagat gctcttcttc acctttcgcg
    13501 tgcaacgaac ggcgatttgc ggagtgcatt gaatgggctg gaactggcta cactatcgac
    13561 tccttcagat aaagaaggaa ggattcatct tacactgtcg atcattgaag aatgtgtcca
    13621 aagaaaagct cttacccatg acaaaaatgg agatgctcat tatgacgtta tttcggcttt
    13681 tcaaaaatcg attcgtggaa gtgatgtgga tgctgcactt cattatctcg cccgtttagt
    13741 tgaggcaggt gatctggcaa gtatttgccg gcgtctgatg gttatcggtt atgaagatat
    13801 aggtttaggt aatcctgctg ctgcggctcg tacagtgaac gctgtattag ctgctgaaaa
    13861 attgggattg cctgaggcac gtatcccact agcagacgtc gtcgtcgatc tttgcttgtc
    13921 gccaaaatcc aattctgcct acatggcatt agatgcagca cttgctgata ttcgagaggg
    13981 caaagctgga gatgttcctg atcatttgag agatagccat tacaaaggtg cgaaatcact
    14041 caaccgtggc gtgggttatc agtatcctca tcattttgat caggcttggg tcaatcagca
    14101 atatttacca gataaactga agaatgctca gtattatcag ccaaaagata cagggaaata
    14161 tgaacaggct ctgggtcaac agtattaccg tataaaagag tggaaaaagc atcctccaaa
    14221 aaactagaca agaaaacggt tctgtttctg cgagctctct cattgctctt tttaaattca
    14281 tatgctacta tagaggtggg aaatctgtga tgttcgaatt gtcctctttg tgtgttgacc
    14341 gaacattttt attgatatct tgggacccgt cttgtgtcac tgctggtaac atgccttatc
    14401 atttggtagt tcgaagctac gaccatggga cccacctgct tgaattcgcg ggatcaatac
    14461 tacaggacaa acaacggcat taacggagat taccatcttt taaagaataa agcaatttgc
    14521 tcttggcaaa ttgctttttc tatttgttgc ttattttgga ggatttgttc atgattagga
    14581 ttttaatgat tatcgccact ttagtattat tattcgttag ctattattta tttaataaac
    14641 aggatatctt ttttgtattg atcaacaaaa atgataaaaa tcaaggattc ctacaatttt
    14701 acggagctgc atatgctgtt ttaggcgtaa tgggtatttt aacagctttt ttcaatcaac
    14761 gctttattgc cttggtcttt ttactgatcg taattattgt ttctgccacc ttcagtatcc
    14821 gatttgcaaa aaaaattgct gaaccaaagc agtaaataat tctctttttc cctaaagtgt
    14881 tctacaataa aagagtacaa tacgattgac ttgaggagtg aatgcttatg ttacaacaat
    14941 acaaaaaaat catggttgcc gtagatggtt ctgatgaagc tgaattagct tttaaaaagg
    15001 cggtcaatgt agctatccga aataacggag aacttctatt agcacatgtc attgacacac
    15061 gttctttcca aaccgtttca tcctttgatg gaatgcttgc agaacaagct actgaaatgg
    15121 caaagcaaac attggcagat tatgaaagca atgcgaagaa agctggcttg aataatgtaa
    15181 cctctgtcgt agaatatggc tcaccaaaac aaattattgc cagagaaatc ccagaagata
    15241 atcaagtaga tttgattatg ttaggagcca caggattgaa tgctgtagaa cgtttattta
    15301 ttggttctgt ttctgaatat gttattagaa acgcagcttg cgatgtgctt gttgtccgaa
    15361 ctgatttaga aaaccaattg ccagctaaag accacaccaa agcttaacac gtagacgacc
    15421 tgccaactat cggcaggttt ttttgcacaa aaaaccgtga taaaaacaaa agacgcggca
    15481 agcactcatc ggtctttata atgtttaaaa taaagggaaa acagtcaatg agttcaagta
    15541 aaataaaaaa aagtgaacac cagggcgatt agtcgccctg gtgtggttta aaacgaccta
    15601 agcatgccaa agcttattgg ttcgtcaatg tggaagtttc cgatgaatct gatacgaaaa
    15661 aatatgaagt atcgatggcg gataccgatt ttatcgccaa actaaaattt tatttcgggt
    15721 tcgggtaccg cgagaaggca agtgctacat tcggtaaatc attcaagaat tgatgaaagg
    15781 ctaccgcttg ccattgcaac cacaacgata ctcctgaacg cgatgttggt gagatttcca
    15841 aagagaagag ctgtttaatg caacggtatt aagcaaatgg caaaaggaag tcatgtagac
    15901 aaagtttttt atcaaacacc atatgatttc gcctgaacgc tggtttagct cagaatacaa
    15961 tgatgtggtc cacgagacat taccctcagt cagataaaaa aagctctctg acagaattac
    16021 acatcgataa atattaagta aatgtcagcg ctagttgaac tttctgacac tttcaggtac
    16081 actggaaaag acaactacac gaaggaggga aaatactatg atgcgcatga ctaaaataac
    16141 aagtaatgaa aattaccaac gataccatat cgcatttagc catgcaattg gttttacagt
    16201 tgctgttgac gggacaagtt tgtccaaaac agttgaattc acgtaaaatc aatggccatc
    16261 tcaaggtatt tttctcatga taatctgagt ttaaataaca caggccatgg ataaccatgg
    16321 cctttttgag tggtctcaag gagagagatc atgatcatac aagcaatttc tattaaaaaa
    16381 acaatcggcg ggaatccatt atttgaggat ttatcctgtc aaattgaagc tggtgaaaaa
    16441 attgccttag tcggtatcaa cggaactggc aaatcaacct ttttacaaat cttgaccggc
    16501 agagaaggtg tcgacagcgg tgttatcagt cgcaaaaaag gcttaaagat cgggacggtc
    16561 gaacaagaac ttacagtttc cgaagcgacc gtcaaccact acttgctcca ttctgccgct
    16621 gagatacagg acctcaaaca gcaaatgagt cgctacgaag cattgatgac agaaccagat
    16681 acaaacttag agaagtgtct ggcccgttac ggtgagctgc agcatcgctt tgaagaacta
    16741 ggcggttacg tattagaaga ccggattgca gcgatcttgc aaggcttagg gattccacac
    16801 cgtcaggaag cacggttaac agaacttagc ggaggcgaac gagttaaagt cgctttagcc
    16861 aaaattttag cagccgatgc cgatttactt ttactcgatg aaccgaccaa tcatttagat
    16921 cttaacagta ttcgctggtt ggaaaactat ttacagaata caaaaaaatc ggttctggtc
    16981 gtttctcatg atcggcaatt tctcgatcaa gtcaccacaa aaacatggga actggaagaa
    17041 ggtgcattga ttgagtatcc cggaaactac agccgttttc gagtcttgaa agaagctcgc
    17101 ttggctgagc tgacaaaaaa ttacgaactg cagcaaaaag aagtccagcg gttgaaagtc
    17161 atgattcgtc gttttcgtca gtgggcacat gaaggagaca atgagtcctt cttcaaaaaa
    17221 gccaaagaac tggaacgccg cctggcgaaa ctgacactcg tcaaaccgcc acctcccccg
    17281 aaaaatcggc tgcaaagctt gtctaatggt ggaaaatctg gcaaagaagt tttcatcatt
    17341 caaaacttgc accagcagta cgctgatcaa gtactattca aagacagcag ttttgcagtc
    17401 tatcgtggag atcatttagc catcatcggc gacaatggtg caggaaaatc aaccttactc
    17461 aaattattac tcggtgagga aaagccttac ggtggcacca tcaaattagg tagtagcttg
    17521 caaatcggtt atttgccgca gcagctgcag tttgccgatc ccgatagccg tttgctggct
    17581 tacgccatca cattgactgg aaacgaagaa gtcgctcgac ggcaattagc caaaagtggc
    17641 ttttatcaaa gcgatgtggg taaacggatc aaagatttat ccggtggtga aaaaatccgt
    17701 ttggcgttgc tgaaactctt tttagaaaaa atcaacgtcc tgattttgga tgaaccaacc
    17761 aatcacctcg acagctatgc tcgagaagaa attgaagaaa tgctgcaaga ctatcaagga
    17821 acgctcctag cagtcagcca tgaccgctac tttttgcagc aacacttcca agaagcactc
    17881 gttattgatc aggagcagat cagccgccag ccgctcggcc aactgattga ctaggacgtt
    17941 ttttcgcaac aaaaaacttg attttcatac ctgtgctttc acgattcagg caaagtacag
    18001 gtggaaaatc aagtttttat tattcgatca atgaatgttt cggatcggtt ttctagctaa
    18061 acataaagcg aagaataaag tcgacagccc acgaggctga tctaaaaatg ttttttcttt
    18121 ttacatatgt aatgtccctt ataaagatga atgaaaataa aatattggga ttttcctgaa
    18181 aatcaataaa taaaatgcca gtcttttccc ttttgaccac ttttccaagc aaaattccct
    18241 aagaagatcc tgtaaaaaag aagggaaatt gaagttaaat taccattttg cggtcttctc
    18301 cgcaaaaaaa acacatgcaa gaattccctc ttgcatgcgt ttcatctttt atcctaacga
    18361 atgctttggc ttagccgcgc agacgctcca catcacgggc aatcattagt tcctcatctg
    18421 ttgggatcac taatactctt acttttgatt cttttgtaga gatatctact tcttcaccac
    18481 gcaattcatt caacgctgga tcgatttcac aaccaaacca agacatacct ttgatgattt
    18541 cactacgtac atgtccgtca ttctcgccga taccagcagt aaagacaatt gcatcaacac
    18601 cattcatcgt tgtcacataa ccgccgatgt attttcggat gcggtccacg aaaatgtcat
    18661 aagctaaacg aatatgttct ttttcatagt tcttttcaag atcacgcata tcactagaca
    18721 atcctgttag gcctagcaga ccagatttct tgttcaaaac attgatcatt tcatcaatat
    18781 caatctctaa tttttccatt aaatatggga gaacagctgg atcgatatca cctgaacgtg
    18841 tacccattgt caccccagct aatggtgtga atcccataga agtatccatg gatttacctc
    18901 catcgactgc tgtgatcgat acaccatttc ctaagtggca agtgatgatt tttagttctt
    18961 cgatcggttt gttcaataat tttgccgcac gttccgacac ataacgatga cttgtgccat
    19021 ggaagccata ttttcttact ttgaaatctt cataataaga caccggtaag ctatataaat
    19081 aattttgctt tggcattgtt gagtggaaag atgtatcgaa tacagccaca ctcaaaatgt
    19141 caggtaaaat ctttttaaac gctttgatcc ccattaagtt tgcaggatta tgcaaaggag
    19201 caaattctgc taattcagca atgcggtcca ttactttctc atcgatcact actgaatccc
    19261 cataagtttc tcctccgtga acaacacggt gccctactcc ggtgatttca tcataagagc
    19321 ttaaaatttg cagttcaatc aactgatcta acagcatttt tactgctact tcgtggtttt
    19381 cgatatccat gatttgttcg aatttttgat tatcgccgta cttgatggta aaaatagaat
    19441 cttgtaaacc gatacgttcg acgatccctt tcgcaactac ggtttcttct ggcatttgat
    19501 ataattgcca tttcaaactt gaactcccag cattgattgc aattgtttta gacatatgac
    19561 tctccttctc gttgaatgat tacagagcag atgatttcca gtttttaaac tcgttgaaaa
    19621 attccgtcac cttctctggt tcttttaatg aagcaagttt cacaagcaag acttctttta
    19681 cttgttgtgc ttgtggtcct tttttctgta agatcaaaat acttttttgc gattgtttat
    19741 tacggaacaa ttcatctggc aattgaatca ttccttgaag ataaccttct tctttaaacc
    19801 attttttgat ttcttcactt tgatccgttt ctaaaaatcc actcggcatc aaaaacaaac
    19861 caaaaccatc cggcttcaca tatttcattg attgctctag caataaatga tgtgcataac
    19921 tatggccttc ttcggtactt gttaaaaact cttttgcttt ttcgtcattc ggataataac
    19981 cgattggcaa atcgctgatt gcaaaatcga caggatctat taataattcc tgcaacccgt
    20041 cttgatgaaa gtattgaaca tttgcttgtg tcaaatcact tgtagaagct gcgacagcca
    20101 acaatgtatc atcaatatcc acaccaattc cttctgtttg gtaccccgca ttcgataggt
    20161 ttaaaagaac tgttaataac aagttgccca ttcctgccgc aatatccaga atcttaaccg
    20221 gtgctttttt atttgtgtat agttgttcaa taagaaaaac aaataaaaaa ccgatactat
    20281 caggagtgag ttgatggttc gcctgcaaat gttctgtctg gctgcccttc aataaaagta
    20341 attgagacag tctgcgccat tcttctggct cgaaagaaag tttcttcaac tcttcataaa
    20401 gagctgtgat ccgttgcgtt gtctcttttg tcggcactcc atccaccaca cgtacttgat
    20461 aatcatctat taaattttct gcattttcga cataggcttc taaaaaggaa gttcccaatg
    20521 cattctgtaa aagctgaata gcttccaggt tttgattaaa cccttgttcc attttttctg
    20581 gtaacatggt gcacctcatt tctagatata ttataacaat tttttcacct gtttaattct
    20641 atcgaacaat tgacaaaatt tcaatcgttt tcactgtttt ttatttgtga aacggcaaac
    20701 atttttttat taaaaacagt gttataacag aaaagagggc gttttcacga acattccatc
    20761 attctgtact agtatctgta tcatttttta cttcttctgt gaaccgaaag ataaacggtg
    20821 agacttccac ctctaagaat agggactgtt gtacccactg atatgttaca gatccagttg
    20881 tgtagtaaaa aactccttcc gaactttcgg caagcgtaga ctgttccgat aaagccatct
    20941 gcttcattat ttttgctata taataatttt tgattcgtat attaagatcc attgacaaac
    21001 ggtagctttc taatgctgcc atcaataaaa acgtacataa aagaaataaa agcaagacag
    21061 aaaataaaat agagccctta taacattttc tactgatcgt tttttgtcca tatggcatat
    21121 ccatcttctc cattttcaaa gtgtacataa aaagaaattg tgtgttcttt ttccaataat
    21181 tgaagctcag tgatacttgt taacataggt tgatgtccgc cggaagttct tcttttacgt
    21241 atcattgcct gatatctttc gatgatatac tgtttatcct cgatcgtgta gtgcagttgc
    21301 tgatttttca cagagacatt gtgactgctc ttgagttctt cttctaattg tgctaaaaat
    21361 attagccact ctttttgatc tttacgctgc aaaaattcac ttacttggat ggtttgctga
    21421 atcaaaagat tgaatgtgag caatagactg ctcaatatca tcagtccaat tagacattca
    21481 atcagcgtaa atgctggaat tacttgtttt ttcaatactg atcgcttctt tctccatatt
    21541 gacaaacact ttttcgatta ctccattatt atcgacaatt tggatatcgt atgggatcat
    21601 ttcttccctt cgctctggtc ttttgtgata catccgctgc gtcttgatat cttcataaat
    21661 cattcgaata aatagcagtt cctccccact tttcttttct gcctgaagca aaaacagctg
    21721 gccaaagctc actaagtaga cacagacaga agcgatcata agcgctgtaa gggactccaa
    21781 caaaacaaat cctgttttat tcaattcttt ttgtatagcg accactccct atctggaatt
    21841 gatacgtaat cgtccgctct ttttcttccc aataaaattg ataagctttt agtgaacttt
    21901 cattcccggt tctagcagca aaagtaatag aagcatggcg tttcaaggta atctcatcag
    21961 gaatatctaa tacttcccaa tttattttat ctggattcgg aacatgaaaa atgatttgat
    22021 tctcttcact attccaataa aagcctgttt ggatctgatt cacaatagcg attttttgcg
    22081 tagaatagat tcgtttttca aattggccga aaaattgata gactgccaat tcattcttcc
    22141 atgcagaaaa tgcgagaact ggaaaagaaa ggatcgttgt aagtaataaa atcagtaaga
    22201 gtgattcaaa caacgtatat cctgatgcta aaaccttacc ttcaaggccg ctgatagatt
    22261 tctgcatgtt ctcgtttgat atagccttca ttcaacaatt cctccacagt aggaactctt
    22321 ccattatttt gaagcgcata gagctctttt tgtgtgtcta ccaactggat gatcgcttca
    22381 cgactttctt ggtctacatg attcctataa cgcgacaaat tagggacgaa caacaacact
    22441 aatacactga tgatcagtaa gacgattagc atttcgatca atgtaaaacc gctataataa
    22501 gatatttttc tctttttcat ggcaaaacct cctctatgct tccgtaaaca ggtaataaaa
    22561 cagcggcata aactaataaa atcaaagccg ctacgcccaa aaaaatgatt ggctgaataa
    22621 aatgcatcca gtgatgaaga cgtttgaaaa gctgttttct tgtccattcg ctgtaaaaaa
    22681 tcagttcttt acctaaactt ccctttgcct ctccctgtaa aacaatttta ctgaattctt
    22741 ctgatagaaa acgataattc tggaactgct cagccaatga catacctgcc tccaatcctt
    22801 gaatcaactt agaggcaaga tgctgaatca gactttcttg tttcgtttcc tttaagcaga
    22861 ctatgatttg ccttaattcg aaaccttcat gaaaaagctt tccaatctct agtgacaaat
    22921 aagccgattg atacaaggaa tatatcggtc caatcaataa ttgtctgctt atccattcac
    22981 tctgatcaac aggagacaac ttcatcagac gataccttac tagtaaagcc aatagagtaa
    23041 ccgctaggat gaatagtaga acataccaat gaaagacttg tatcagctga ataccccaat
    23101 gctcttgcat gaccattcct gaaccaataa gctgaggcaa aacgaacaac cgcatactaa
    23161 acaagatacc tagcaaaaag gtgagcaata gcatcggata tgcaagcagc tttttcaaat
    23221 cattgcgaaa tccttcaaca agccgaaact gtttggcaat ttccaacaaa gttgtcggca
    23281 gattcccatg acgttcagct aactcgatct gtaccagttg ctctgctcca tatcctattt
    23341 gcgaaagtac ctcactaaac gcttgccctt cctttagctg atgttggata tttgccaaaa
    23401 taacaggtgg aaacgaacgg atcgtgagta ataaataaag ggattcttgc agactaaaac
    23461 cagataaaag cagttctccc atggtttgtg caaactggtt ttgctggctt cttgttaatc
    23521 ctttagaata gttctgactg ccaaatattt tcctgagctt cttcataact ttcctcccat
    23581 gattttttta ctgattcttc ctccataaat tgataactcc ataaaacaga aggatggtga
    23641 ttggcatctt ccaaaagttc ttgataaatg acaccagcta aacattcaga tagctcttct
    23701 tttttctttg tcagttcatg gattctgacc aatgtacctt caagattgcg tgcatgtacg
    23761 gttgcaaaaa cccggtgccc tgttagtgct gctcttaccg cagcatgtgc agttgattca
    23821 tctcttatct cgccaatgat tagcaagtct ggacgatgcc gtaatgcgag tctcaaaagt
    23881 gtatcataag tctgtgctat tttcggattg atctgtagct gcagaaaaaa cggttcttct
    23941 atctctacag gatcttcgat agtaatcact tgcaattttt cttccaaagc caaccgatac
    24001 attagagaag tcttgcctga tccgactggt ccgctgaaaa ggtataatcc acgtctgacg
    24061 gttttctgta aaatagttgc aaaatctcga gaactaaagc aacgatgggg tgtttcaaaa
    24121 gtaccgtata atagccgaat cactaaactc tctcgttgaa ggtaatctcc tacagaagac
    24181 aaccgcagac gaacttcctg tttttctaaa gaataagtaa atgcacctaa ctgagctttt
    24241 cttttttctc cgatatccat ctgtcccaaa tacttaaatc ttgagatcag ttgctgtcca
    24301 tctgccgaag agactttctt ccagaatgtt cgtttttttc catagcgaaa atagatgtaa
    24361 aatacttctg aagctggcaa tatatatacg tcttgggcat gtttggcaaa actttcactt
    24421 atcatttgat ccgataatcg ttttatttcc attattctcc cctccacaaa aaacatacgt
    24481 ctttttgatc tttttgtttc aaataaaatg aatttacaaa aaaatattgg caaaaaccga
    24541 atttatgtta tacttataaa aatttatctt gggaggcact ccattggaaa caacattaag
    24601 gaaatatatc gaagtagcaa gtaaaaaaaa gcctgctgac ctagtgatca agcatgctaa
    24661 gatcgtcaac gtctttacga aagaaatcat ggaaaaagat gtagcaatct gcgaaggaat
    24721 gatcgtagga atcggtgaat acgaaggaaa aagcgtacat aatgctaatg ggcaatatct
    24781 agtgccagga ttcatcgatg gccatgtgca tatcgaaagc tcacttttat caccaagcga
    24841 attttcaaaa gtatccttgc ttcatggagt tactactgta gtgacagatc cccatgaaat
    24901 aggaaacatt gctgggagta caggtcttga gtttatgata gaagatgcaa gacaaacact
    24961 catgaatatc ttttttatgc taccttcttg tgtacctgta accccttttg agaccaatgg
    25021 tgcaaattta aatgcaaaaa aattagcccc ttttttacaa aaacccgaag ttttaggctt
    25081 agctgaagtc atgaactatc aagcagtagc aaataatgaa acagacatcc tagaaaagat
    25141 ccaattgatg caccagcata agaaaaaaat tgacgggcat gcagctggta tcggcatgga
    25201 agagttaaat gtttatcctg cagcaggtat tcggacggat catgaagcta caactgcaaa
    25261 agaagctaaa gaacgtttgg atcttggtat gtatttaatg gttcgagaag ggaccgttgc
    25321 aaaagatctg gcatcgctac taccggcgat cacacctgaa aattcaagaa gatgcttctt
    25381 tgtcaccgat gataaactga tcaatgattt agtaaaagaa ggatcaatcg atcatatcgt
    25441 ccgtcaagct attgcacttg gaatcgatcc attacaagcc attcagatgg caacattgaa
    25501 tgcagcagaa tgttttgatt tgaaaacact aggtgcgatt gcccctgggt atcaagctga
    25561 tttcttctta acagacgatc ttaccaagct gccaattcat gaagttttct ataagggaac
    25621 attagttgtt caatctgggc aactccaaga ccatttattt gctgatcatt caaattcata
    25681 tacgtgttgt ctgccaaaac taaacgctca accattaaat ccacattcgt tagagctgct
    25741 tctttcttct tcacaagccc atgtgatcga aattatcccg aatagtctag tgactaaaga
    25801 tttggtagaa aaagtggacc gacaaggcga acgattcatt ccttcaatcg aaaaagacca
    25861 attaaagatt gcggtgatcg agcgtcacca tcaaacggga aacattggat taggcatagt
    25921 gaaaggattt cagctgaaac aaggagcgat agcgacaacg attgcccatg actcgcataa
    25981 tctagttgtc gttgggacaa acgacgagga tatgctgtat gctgctaatc ttttgattca
    26041 aaaaggtggc gggatgaccg ttgtcaacaa acagcaggaa atagcttgtg tttctcttcc
    26101 tataggcgga atcatgacac aggaaccttt ttcgatcgtc tatgaacagt tgaatgaatt
    26161 aataactaaa gcacatcaac taggagcagc taaaaccttt gatccttttt tgacactttc
    26221 tttcttaaca ttatctgtca tacccgaatt gaagatcacc gacaaaggat tattttcatt
    26281 ttctcgcttc caattgattc caccttgtgg aaaaaccgat gaaataaata cattttaaat
    26341 caaataaatc ccagaaaata cattacatct gtaatgttca ttttctggga tttatttatg
    26401 gatattaatg tttttatgaa agcagaaagt tagtttcaca gacttttctt tttggtctta
    26461 tcttgaaatt gagcttctac tcgataaata cgttgacctt ttgaagaaaa tttttcttca
    26521 tactccgtca taatattgcc ttcaaattga ctttgatgta aatctaacca tacctgtttg
    26581 aggatcatgc catattgaga aaaacttgcc agagaatact caaacaatcc ttgattatct
    26641 gttttgaaat ggatttctcc atttggacga agaatctgtt catctacagc aagaaaagtc
    26701 ttatatgtca atcgccgttt ttcatgacgt ttttttggcc atggatcgga aaaattcaga
    26761 tagatttgat ctacttcgct gtcagcgaaa tactctgtca acgctgatcc atccacatgt
    26821 agaagtttca ggttaggaag cggttgttcg atcaacttgt ctagtgcaat cgaaacaaca
    26881 ctgacctgca tttctactcc aatataattg atttcaggat gggcttttgc cattccagtg
    26941 atgaactggc ctttccccat accaatctca atgtggattg gatgatcgtt ttcaaacaat
    27001 tcattccatt ttcccttcca caatgttgga tcactaatta caaaattcgg gtgagccgct
    27061 aacatttccg ctgctcccgg acgatttcgg acacgcatta tttatctcca ttcctttcct
    27121 ctaattaaaa aggtctggcc aaatctccgc agagtttggg ccaaacctca gccactgaaa
    27181 attcgtttta attgtaaaat ttcttcgttc atccgtcgat gatcttcacg tttgcaatat
    27241 ctggtgattt cttggagcaa attcatggcc gcataccaat gaaatttttc aagcatttcg
    27301 tcagtcgggc gtatgccata actgatcagc cactgattcc agctagacag tggcacataa
    27361 tttcctaaaa tcgttgcaat atccaccgca gggtctgcaa acatgattga atcccaatcg
    27421 actagatata aataattttt acatactaac caattacgat ggtttacatc tccgtgtact
    27481 actgataagt tttccttatc tattgacggg atctgctcac gaagaaaacg atggacaatt
    27541 tgcagaaatt gatttttctg gagttcttcc ggtaattctt tttcatattt ttgcagcatc
    27601 ttctccggcg tcatgacacg accaccgata cgggtcagca tggttttaag agaatttgaa
    27661 tgatgtaaat ggtacaaaac atcaatcacg tcgtttcttt gaccaatctc attaggagtc
    27721 agcaagcgac cttccagcca ctcttgtgct gtcaaagtat cgccattccc cgttcgtttt
    27781 gtccaaacaa gttttggtgt aattccttcc cttgaaagag cagccaacat cggagtcgtg
    27841 tttcgtttaa taaagacttt gtcctcatca cgcatgccta tgtatgtttt tcctgtgtcg
    27901 cctttgatgg gacgtaaccg ccactcttgg tctaactgaa actccatcat gcattctccc
    27961 gtcctcttac tctttgtttt gcagtactta ttattctagt cgcctgaaca ctacacgtca
    28021 agcacatata cgttcttttt catttaattg ccattcaaaa aaccagcttt tctcaaaaaa
    28081 aatagaacga acagcattct tactgtcaac gctctatttt tcattctcat ttttacccta
    28141 cacagctttg ttttcagtct gacatcttct tcaaacgagt tggtacatat agtttaacaa
    28201 agagcatagt aacaatcaaa taagccgcta gcggaaccca tcgctgctgc ccatctaata
    28261 cgactgcagc aatcaaacca aaaataaccg acgcgataat taatacccat gtgagtagat
    28321 actgaatcgc ctgcttcttc tgattcttat ggattggata aagctgaacg agaaccatat
    28381 agcggaactg gttcgctaaa ggtaacagtt ggaaaccgat caaatataaa aataagaaac
    28441 cgataatcag actaaaataa aagtcgacta ctccggcaat caataaccca cctataatta
    28501 ctaatcggaa ataaagacca ctaaaatcag aacctcttgc caatcttctt gcaaacaagt
    28561 aaagataagt gttttttgat tctcttttaa tatgagtcaa tataggatcc aaatagcgtc
    28621 ttcttttgat ctttgccgta atttcaggaa catctgtgaa taaattgata aattgataga
    28681 tctgatgcaa cctatgttct tctacatgca ccattttctc ccaatccaat cctttttctt
    28741 gttgcttcca caaaaaccag taatatagac ttgcctgtac taatgcacct gctattcctg
    28801 cccatattag ccaataaaat gatagaaaca aaaagatcag agaagaaaaa aaccagccta
    28861 atttccaata acgaataact tgttgatcta gtcgaaacaa ttctgctctc ttgatttgca
    28921 gatgactgaa tttcaaactc cataatatga tcagatagac gaaaaacatc gaaaaggatt
    28981 gctccgtact aaccaccact aatggcatgg ccaatccgca tgccaatatc aaaaagatca
    29041 acggaaaaat gcaagaatag cgaaaagctc gttccaaata ggtttgcatt tctttttctt
    29101 taggaagtaa aaaaacaata tctgctgcct ctgcaaaagt agccatctta cccacgaata
    29161 aacagagcat ccaaaataca gagattatga gccctccaat tgcaaacggt ggctgcaggg
    29221 ttttcaacca attagaataa tataatccca caccgccaat caagaaaaga caaactaaaa
    29281 caaaatgatc gttcaataca taacgcatat atttcatcat tttcttttga tgtcttgcta
    29341 aacgtttttg aaaaaaacta ctcatgtgcc tgctcctttg ttaaagcaat atagatttcg
    29401 tccaaagaag aatccggcag ctgaaattct tttctcaaca tctcaagaga accgtctgct
    29461 cgtatctctc cgttatgtaa gaccacaaaa cgatcacaat atttttctgc agtagctaaa
    29521 atatgcgtag acatcaaaat accggctcct tcttttttca tttctaccat caattcaagc
    29581 aatgcattga tagctagtgg atccaagcct aaaaatggtt cgtcgatgat atataaagaa
    29641 ggctcgatca aaaaggcgca taagatcatc actttctgtt tcattccctt tgaaaaatta
    29701 gctgggaacc agtcaagacg attagataac cgaaaagtct ccaacaactt ttctgctctt
    29761 ttcattgcta tatccatagg tatatcgtag gccatcgcag tgacttcgat atgttctctc
    29821 aatgtcaatt cttcatataa agaaggagtc tctggaatat acccaatttg tttgcgatat
    29881 tcttccgtat ttttctttaa ggatagatga ttgatttcta tcgttccttt tgaagcttcc
    29941 agcagaccga taatgtgctt gatggtcgta cttttccctg ctccgttcag tccaatcaac
    30001 cctactatct ctccaggctg tacatcgaaa ctgatatctt ttaacacagg gagatgtccg
    30061 tacccccctg tgacatgatc gataattaaa ctcattcttt ttcctccgcc atttattttt
    30121 atttattata ccacgaagaa aatgacggta ttttgttttt ttccttacaa tagctttaac
    30181 ttatggtaaa gttaagctga agtatttatt ctttatagaa aggatgtttt atatggaaaa
    30241 ctgtattttt tgcaaaatca tcaatcaaga aatcccaagc tataaaatat atgaagacga
    30301 caaggtctat gcttttttag acatctctca agcaacgaaa ggacacacat tagttgtgcc
    30361 taaacgtcat gtagcagata tttttgaata tgatcccgaa cttgcaggag aggttttttc
    30421 gcgtgtgcct aagattgctc aagcattgga aaaagctttt ccagaaatga aaggtcttaa
    30481 tatcttgaat aacaaccgtg aactagccta tcaatctgtt tttcattcac atatccattt
    30541 agtccctcgc tacagcaaag aggatgattt ttctattcac tttgtcaatc atcaagatag
    30601 ttatggatcg gaagaactaa aagctattca agaaacaatc gtaaagcagg tgtcctgcga
    30661 tgattaaaaa ctttttcaaa ggactgttat ttggagctgc tgctggcact gtcggaggtc
    30721 tgttattggc gcccaggagc gggaatgaaa caagaaagaa actgatcgaa gaattggaag
    30781 aagcaacaac tctgacaaat gatttaaata acagcctgaa tcattttaaa agcgcattga
    30841 tcgaaacaaa agaaacagct gaatctgtga ttcctgcttt ccaagaagca atcaaaaaag
    30901 atatcgaaga ttttaaattc caagccgaac cacgtatcac acaaatcaat gaacaagtag
    30961 aaaaactaaa a
//
LOCUS       DANKAY010000023        30969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000023 DANKAY010000000
VERSION     DANKAY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(415..1350)
                     /gene="whiA"
                     /locus_tag="PWY98_001046"
     CDS             complement(415..1350)
                     /gene="whiA"
                     /locus_tag="PWY98_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2036811.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1384..2382)
                     /locus_tag="PWY98_001047"
     CDS             complement(1384..2382)
                     /locus_tag="PWY98_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2036812.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2379..3263)
                     /gene="rapZ"
                     /locus_tag="PWY98_001048"
     CDS             complement(2379..3263)
                     /gene="rapZ"
                     /locus_tag="PWY98_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2036813.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3398..4129)
                     /locus_tag="PWY98_001049"
     CDS             complement(3398..4129)
                     /locus_tag="PWY98_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2036814.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4133..5398)
                     /locus_tag="PWY98_001050"
     CDS             complement(4133..5398)
                     /locus_tag="PWY98_001050"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2036815.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5661..8477)
                     /gene="uvrA"
                     /locus_tag="PWY98_001051"
     CDS             complement(5661..8477)
                     /gene="uvrA"
                     /locus_tag="PWY98_001051"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2036816.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8487..10481)
                     /gene="uvrB"
                     /locus_tag="PWY98_001052"
     CDS             complement(8487..10481)
                     /gene="uvrB"
                     /locus_tag="PWY98_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2036817.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            10740..12242
                     /locus_tag="PWY98_001053"
     CDS             10740..12242
                     /locus_tag="PWY98_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2036818.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12244..12981
                     /locus_tag="PWY98_001054"
     CDS             12244..12981
                     /locus_tag="PWY98_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2036819.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13152..14345)
                     /locus_tag="PWY98_001055"
     CDS             complement(13152..14345)
                     /locus_tag="PWY98_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2036820.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(14424..14630)
                     /locus_tag="PWY98_001056"
     CDS             complement(14424..14630)
                     /locus_tag="PWY98_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2036821.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(14623..15765)
                     /locus_tag="PWY98_001057"
     CDS             complement(14623..15765)
                     /locus_tag="PWY98_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2036822.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            16277..16912
                     /locus_tag="PWY98_001058"
     CDS             16277..16912
                     /locus_tag="PWY98_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2036823.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            17066..17143
                     /locus_tag="PWY98_001059"
                     /pseudo
     CDS             17066..17143
                     /locus_tag="PWY98_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            17388..17504
                     /locus_tag="PWY98_001060"
     CDS             17388..17504
                     /locus_tag="PWY98_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2036824.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            17520..19439
                     /gene="tet(M)"
                     /locus_tag="PWY98_001061"
     CDS             17520..19439
                     /gene="tet(M)"
                     /locus_tag="PWY98_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDL2036825.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            19558..19725
                     /locus_tag="PWY98_001062"
     CDS             19558..19725
                     /locus_tag="PWY98_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2036826.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(19785..20138)
                     /locus_tag="PWY98_001063"
     CDS             complement(19785..20138)
                     /locus_tag="PWY98_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036827.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            20642..21061
                     /locus_tag="PWY98_001064"
     CDS             20642..21061
                     /locus_tag="PWY98_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2036828.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            21087..21293
                     /locus_tag="PWY98_001065"
     CDS             21087..21293
                     /locus_tag="PWY98_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036829.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(21521..21694)
                     /locus_tag="PWY98_001066"
     CDS             complement(21521..21694)
                     /locus_tag="PWY98_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036830.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            21670..22335
                     /locus_tag="PWY98_001067"
     CDS             21670..22335
                     /locus_tag="PWY98_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036831.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(22417..23583)
                     /gene="dnaJ"
                     /locus_tag="PWY98_001068"
     CDS             complement(22417..23583)
                     /gene="dnaJ"
                     /locus_tag="PWY98_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2036832.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(23734..25563)
                     /gene="dnaK"
                     /locus_tag="PWY98_001069"
     CDS             complement(23734..25563)
                     /gene="dnaK"
                     /locus_tag="PWY98_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2036833.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(25614..26177)
                     /gene="grpE"
                     /locus_tag="PWY98_001070"
     CDS             complement(25614..26177)
                     /gene="grpE"
                     /locus_tag="PWY98_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2036834.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(26271..27314)
                     /gene="hrcA"
                     /locus_tag="PWY98_001071"
     CDS             complement(26271..27314)
                     /gene="hrcA"
                     /locus_tag="PWY98_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2036835.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(27548..28713)
                     /locus_tag="PWY98_001072"
                     /pseudo
     CDS             complement(27548..28713)
                     /locus_tag="PWY98_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(28729..28956)
                     /locus_tag="PWY98_001073"
     CDS             complement(28729..28956)
                     /locus_tag="PWY98_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2036836.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            29440..30030
                     /locus_tag="PWY98_001074"
     CDS             29440..30030
                     /locus_tag="PWY98_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2036837.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            30220..30933
                     /locus_tag="PWY98_001075"
     CDS             30220..30933
                     /locus_tag="PWY98_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036838.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         8973 a         6313 c         5556 g        10127 t
ORIGIN      
        1 aacttctaca ttaattacac ctggttgttt tcgactagct aatccaatga acttatcaac
       61 catccttatc caccaaccac gagaatcttg accagaagta agaaaaacag ttctcaaggt
      121 tgatcgagca gttccttcca gtttttaatt aatctccagt tatagataaa tacttgatcc
      181 tatctatatc aggacttcta tattaagcaa aagtttatgt ataaaactaa aatcttcatt
      241 cagttgtctc cccgcttttt atacgattgt attaaaaaac aatagaagcc tttcaacatt
      301 atgttactcc agtaaagttg gaattatagt ttgacggaag aaaagcaagt atagctttta
      361 aaaaaaactg atacattcgc atctcctgct tcatgtatca gttttttctt tggattaaac
      421 tgcttcttca cgcagttttt ccgcaaattc atttattttg cgaattcggt gattaattcc
      481 cgattttgat attgcacctg agggaatcat ttcccctaat tctttcaagc tgacctctgg
      541 atgttgaagc cgaagttctg cgatttcctg aagtttttca ggaagggcat gtagaccgac
      601 ctttgcttcg atcaattcga tgttttcgat ttgtttagaa gccgcatcaa tcgtcttgtt
      661 cagatttgct gtctcacaat tgactagacg attgaccgaa tttctcatat cccgtacgat
      721 tcgaacatct tcaaatcgga gcattgagtt ggttgcacca atcaatgtta agaaatcagc
      781 aattttttct gctcctttta aatacgaaat atagccattg cgccgttcaa gcgttcgagc
      841 atgtagaccg taataattta acatatcgca aatgtcttgg ttatgctgtt cataaataga
      901 aaatatttcc aaatgataac gactcgtttc cggattattg actgaacctg aggccataaa
      961 agcaccacgt aagtaagaac gcattttttg cgaatttccc ataatctcgt ccgaaacgtg
     1021 tccattgaac atcataccat ccatgatatc aagatccatc aacacatctt ttgtgccttg
     1081 cttcaaccgg acgatataga cattgttctt ttttaatttc atttttttac gaacgagtag
     1141 ctcacttcgt acctgataat gatctttcaa aagagaatag atacgtctgg caattgcagc
     1201 attttctgtt tggacgttca aaataaactg ctggttcaca atacctaaag agccgttcat
     1261 tcgcagcaaa gcagctaatt ccgctttcgc atgttctcga tgaacttcca aaccagttag
     1321 ttctttcttc acatctgcag caaatgacat cctattctcc tcctttctcc aacaataaat
     1381 tgcttaatat tttgaaccga aaactaaacg gaacaattct tctactactt tgtctccatc
     1441 gtggaagaca ccaccatctt tcaattctaa aaagtcggtg gaaatcactc gacatccttc
     1501 atttctcaat ccgttgaagt cgtgtgcgac ttgtactaga tactcatcat agatctctgg
     1561 gtccatataa tcttcaggca ctttttccgt attgaccaat actgtatcga taaaaggctg
     1621 attcaaatgc ttgtgtaaaa cacgtatatg atcggcatct gtgaaatgtt ccgtttcccc
     1681 tttttgggtc atgatattac agatatagac ggtttcagca gttgtcttga gcattgcttc
     1741 gccgatttct ggtatcacta aatttggaag gatactggtg aataaacttc cgggtcccaa
     1801 aacgaccatg tcggcgtcca taattgctga aacgactttt cgagccgctt tggcttcact
     1861 tttatcattt gtgttgcaga catacacacg gtcaatggtt ttacggttga tcgcgatttt
     1921 agattctcct actgcagtcg ttccgtcttt aaaaacagca tgcaaggtca aaggagtttc
     1981 tgaagaagga taaacatgtc catctacatg catcattttt gctaataatt gaatcgcctc
     2041 ataagtgctt ccccgcatct cggaaactgc ggcaatgatc agattaccga tcgtatgatt
     2101 ggctaaaaac tgatcttcct ttttgaaacg gtattggaaa atatcttcat agaattgtgg
     2161 catatccgat aacgccacta agacatttct caagtcacca ggaggagcca tagcgatcga
     2221 ttctctgatt gcaccgctgc ttccaccatc atctgcaaca gtcactactg ctgtgatatc
     2281 tgctccttga tttcgtaaac ttttcaggat aactggcaga ccagtgccac cgccgaccac
     2341 tacgatcttt ggttttctta tccggtaggt tttcattttc acgaccggtt caccgtctct
     2401 ttccttttat ccttgtcccg atgtgtcaca ttgactttat agtttttccc taaagcattt
     2461 gccaaacgtt cagtcaatgc cacagaacgg tgttgaccgc ctgtacagcc gattgcaact
     2521 gtcaagctgc tttttccttc tttaatgtat ccaggcaata cagtttccag aagatccaaa
     2581 aatttatggt agaatgattg cgtttcatca aaggacatca cataatcata gactggctga
     2641 tcttttccgg tcaatggtct tagttccggg atataatgag ggttgggcaa aaaacgcaca
     2701 tccatcacaa tatccgaatc gatcggcaac ccgtatttaa aaccaaatga tacaaattcg
     2761 atccgaaatc cttggtctcc ctttgatttg aatgactcat tgattttttc acgtaattgt
     2821 cgtggtgtca atgttgtcgt atcaatgatc acggaagctt gggctttcaa gtcgtctagt
     2881 atcgcccgtt ctttacgtat cccttcagtg atcaaaccgt ccatcgccat tggatgtgct
     2941 cgtcttgttt ctttgtagcg agccactagt tcttcgtctg aagcatctaa aaataagata
     3001 gaagtgtcga tgaagttcgt attttcgatt tcgatcagca tgttttggat ttcttcaaag
     3061 aaggaacgag aacgcaaatc cactcctaaa gccatttttg tgactttacc tgattctttg
     3121 atcaattccc agaattttgg aatcaatcga ggcggcatat tatcgataca aaaatagccc
     3181 atatcttcaa aactttggat agctactgtt ttccctgcac cactcatacc tgttatgatg
     3241 actaattgca aattatctgt catatttacg aacccctctc acattgtgca taataaaatt
     3301 atatcatccc gggcgtatca ttaccagttt tctttttctt ttacaaaaag atttggacca
     3361 aatatctttt aggatatttg ccccaaatcc aattttttta cttttccgat gctgtatcca
     3421 atgcctcgct tctttcagct aatgcacgtt cgatcgttcg atccgctaaa atagattgag
     3481 catcgatcac tgggtaatga ttatcttcag caaattcatg catcaaagat aattctgtgc
     3541 aacctaggat gactttttca cagtttaggc gctctaccgc ttcctctaag atttcgtaat
     3601 agagctcttg attcaaataa tccgattcct tgatctcatg atagatcagg taattgattt
     3661 tttcttgaag cgcagtatca ggaatcactg tttcgaaacc aagatttttg acttcgcgtt
     3721 catagattcc agctttcata cttccttctg ttcctagaat cgctactctc cctgtcgtat
     3781 gctgacggac aagttcattt gctgcttctc ttggcatatg caagatagga atatccgttg
     3841 ccgcttgtaa ctcttcaaaa aagtagtgtg cggtgttaca agtcaacaca atgaagtttg
     3901 gtcgcaataa attttgtttc tcaatatcat ccagcagaaa cggcattgga ttttcttctg
     3961 atcgatctaa aatataagct gtacgatcag gaactgttgc atgattgaat aagacataat
     4021 tcaaatattc ttgatctttt gtagctttcg ttcggtggtt gatcaagcga acaaaacttt
     4081 ctgtcgccat cgtgcccatt ccgcctaaaa tactgaaaaa attctccatg aactagcctt
     4141 ctttcacatg aaaatacttt ttgaatcttg gaatatagtt atggaacata tatttcatca
     4201 acaaccagcg cttgattgat cggtcttttt cataaaatac agtagtaccg taacgatttt
     4261 ctttgatcat ttgctcagct agttttttgt cttcattttc ccgagcatat tctaaaaaga
     4321 tttttttggg cacacccatc cataatcttg ctttatctga ttgattcgtt ccataggtcg
     4381 tttcaacaaa gggtttgttg aacactcgat cttctgtgac aaaacgagcc agattcaagc
     4441 cgttcaatgt cacaaagaaa ctgctacgac cttgtctcaa attgatttca aataatttat
     4501 attctccatc tctaggatca tacttcatat cgaaattggc aaagcctgta tattcgattt
     4561 tttccaaaaa tgccttaatg gtttgataga ttttttcgtt ataatctggc atgatcacaa
     4621 cgtaattacc aatcgatgct ggtgtaggat cttctaatag tggatgacca aggcacatca
     4681 tccgaacttg atgatcttca tctacatacg catttagcac acgcatatta ctatcatcac
     4741 ccgggataaa atcttgtaca atcatttcac ttttataacc ggcttcgtat atccggccta
     4801 aaattaaatc gaactcttct ctagtctcta aaataaaagc tttttttctt ccttcaaatt
     4861 ggacagaaag atactccaca ctgtttgcag gtttcaaagc aactgggaat tcaaaaggca
     4921 attcctgttc caaatgtcca tttactaaca tttcttctct gacgatcaat gttttaggat
     4981 acggaagatc atattcttca caaacttcat aaaagcttac tttattgatc agcctttcaa
     5041 ataaagaata atcaatatat ggacagataa aatattctga cagctcttgt ttatgttggg
     5101 agatcaattc tgcatatcca tcaccacaag caatcaaaag atatttttta ctcttatcag
     5161 aatatctttc ttttgccaaa cgaagcatcg tctcgataaa tacagggtct ttatcaaatc
     5221 ctggaataac ttctacattg acaattttac tgtagcgggt aggggctaac tgatccgaag
     5281 catatgcttg gcagattttg ccgtatgctt cattaaaaga acgtgccata ccataaacgt
     5341 tcatgtcgct tcctaataat atagggataa attcttcatt ttcaatactg ttcatcatta
     5401 tctctcactt actcataatc tcttgttttt cttttccaac tacacaagga atatcatacc
     5461 ataaagaaaa aagcaatcaa aggaaagtcc tccgattgct caattctttt ttgaacggat
     5521 tgaacttata ggaagttttt atctatacca atcagtgtag aaagaactac catgtaatga
     5581 ttctagaaca ctgatattaa taaaacaagc aaaaattcat ttttttatgt ttttttaact
     5641 atctcagcta tctatcctct ttataccatt atttttttca aatagtgacc tgtataactt
     5701 tcttttacgt taatgatttc ttctggtgtt ccagtcgcaa ggatcgttcc tccaccttct
     5761 ccaccttctg gtcctaaatc aatgatataa tctgctgttt tgattacatc tagattgtgt
     5821 tcaatcacta aaactgtgtt accagcatct actaatcttt gtaatacatg caacaagcgg
     5881 gcgatgtcat cgctatgaag tcctgtcgtt ggttcatcta gtatatagaa attctttcca
     5941 ttagagattt tgtgcaattc actggcaagt ttcatccgtt gtgcctcacc accggacaat
     6001 gtcgttgctg gttgccccat cgtcacataa cctaagccaa catcaacaat cgtttgcagt
     6061 ttgcgatgaa tctttggaat gtgcttgaag aattctacag catcctctac tgtcatttcc
     6121 aaaatatcag caatgctttt tcctttgtaa tgcacttcta atgtttcaga attatatcgt
     6181 ttaccatgac atacttcaca aggaacatag acatcaggca aaaagtgcat ttcgatctta
     6241 ataattccat ccccgcgaca agcttcacaa cgaccgcctt ttacgttgaa actaaaacgc
     6301 cctttcttat aaccccgcat ttttgcctcg ttcgtttgag caaataaccc acggatatca
     6361 tcaaatacac ttgtataagt cgctggatta cttctcggcg tccggccgat tgggctttga
     6421 tcgatatcga tgatcttttc gatactttcg tagccggaaa ttgtcttgaa tttacctggc
     6481 ttagcagaat tcttatttaa tttttgcgct aacgatttct ttaagatact attgaccaat
     6541 gtactctttc cagaaccaga cacacctgtg actgctacaa attctcctaa tggaaattca
     6601 acactaatat ttttcagatt gttttctgaa gcaccagtga tcttgattgc tttcccatta
     6661 cctttacggc gttcttttgg tacagggatt gattttttcc ctgaaagata tttccctgtc
     6721 aaagaatttt catcattagc tacttcttca ggagtgcctg cagccacgat ttgtccgcct
     6781 tgttctccgg ccccaggtcc gacgtcaatc aaataatctg ctgcccacat cgtatcttca
     6841 tcatgttcga ctacaacgag ggtgttgcca agatcacgca ttttctttaa tgagccaatc
     6901 agtcgatcgt tgtcgcgttg atgcaatccg atcgatggtt catctaaaat atacaaaact
     6961 ccagaaagat tcgatccaat ttgtgtagcc aatctgattc gctgcgcttc tcccccagat
     7021 aatgttccgg aagcgcgact caaagttaga tagtctagtc ccacattttc caagaatgaa
     7081 agtctgtcat ttacttcttt taaaatcggt ttggcaatta ctttctcttg ttcagataac
     7141 gttaagtttg agaagaaatc tactgctttt tgaatagaca actcatttgt ttgcccgata
     7201 ttcgttccat tgatttgaac agatagagct tggggattca atcgataacc tttacatgtc
     7261 tgacaagtta attctgtcat ataaagacgc atttgctcac gcgtgaaatc actgttcgta
     7321 tcatgataac gacgctcgat attcttcaat accccttcga aagtcgtttc tacatcgcga
     7381 acgccaccaa aatcattttc ataatggaaa tggaaaagat catctgtccc attcaagatg
     7441 atctcctgat gctcttccgg taattcttcg aatggtgtat ctaaatcgat accgaaactt
     7501 tcacaagcct gcgctaacat ttgaggataa tactgagagc tgattggatt ccatggaacg
     7561 atagcacctt gactaagtgt cttcgttcta tctggaatca ctaaatcttg atcgacttct
     7621 aatttgacac ctaaaccatc acagtctgga caagctccaa aaggcgcatt gaatgagaac
     7681 agtcttggtt ctagttcgcc aacagtaaat ccgcaataag gacaggcata gtgttcgcta
     7741 aacaacattt cttcttcacc gatcacgtca accagagcat agccgtctgc taatcgcagg
     7801 gccgcctcta aagaatcaaa taggcgagaa cgaatccctt ctttcacaat gatccggtcg
     7861 atgacgatcg cgatatcatg ttttttgttt ttctccagct caggcacttc acttaaatca
     7921 tagatctcac catcgactcg aacgcggacg tatccctctt tttggattcg ttcgaatatt
     7981 tttttatgtt gtcctttttt ctttaccacg atcggtgcca acacttggat ctttgtgcgc
     8041 tcaggcaaag ccattacttg atctgccatt tgatctactg attgactggt aatctcgata
     8101 tgatcgttgg gacaaatcgg atgtcctaca cgtgcaaaca acaagcgtaa atagtcattg
     8161 atttccgtca cagtacctac tgtcgaccga ggatttttac ttgttgtttt ctgatcaata
     8221 gaaatcgccg gactcaatcc gtcgatgcta tctacgtccg gcttatccat ttgtcctaaa
     8281 aactgacgag cataagcaga aaggctctct acgtatctcc gctgcccttc agcgtacagc
     8341 gtatcaaatg ctagggaact ttttccagaa ccggacaaac ctgtaacgac cacgaattta
     8401 tctcgtggaa tcgttacatc tatatttttc aagttatggg cacgtgcacc atgaatcgta
     8461 attttatcgt tagccatgtt gtcctcctac tttgaagctt ttaattctaa aatcatatct
     8521 cgcacattcg cggctgtttc aaaatccaaa gcttttgccg catctttcat ttctttttct
     8581 aatttcatca ataacgtgtc tttttcttcc ttcgtcattt cttcgtacga gacttgtacc
     8641 gcttcttttg tatcatcttc cgattctttc gtaatagaaa tcaaatctcg gatttcttta
     8701 atgatcgttt ttggaacgat cccatgttct tcattgtact tctgctgaat cgtacgacgt
     8761 cttgacgttt catccatcgc tagacgcata gaatctgtta ctttgtctgc gtacatgatt
     8821 actttacctt cttcattccg agcagctcgt ccaatcgttt ggactaagga acgttcacta
     8881 cgtaaaaagc cttctttatc cgcatccaag atagcaacaa gggaaacttc aggtacgtct
     8941 aatccttcac gaagcaaatt gattccaact aacacatcaa attcacctaa tcgaagatct
     9001 cgaatgattt ctgttcgttc cagcgttttg atatcactat gcaaatattt gactttgatc
     9061 cctaattctt tgaaataatc tgtgaggtct tctgccattt ttttcgtcaa tgtcgttaca
     9121 aacacccgct ggtctttttc gactcgttca tttatttcgc ctactagatc atcgatttgt
     9181 cccatgattg gacgaacttc gatcactggg tcaagcagcc ctgtcggacg gatgatttgt
     9241 tggataacgg tatctgtttg ttcatgttca tatggtcctg gcgtcgcaga gacataaatg
     9301 atctgattga catgtttttc aaactcttct aaccgaagtg gccgattgtc taaagcagat
     9361 ggcaaacgga aaccgtaatc tactagcatc tgttttctcg ctcggtcacc attatacatt
     9421 cctctgattt gaggcatggt tacgtgcgat tcgtccgcca cgatcaaaaa atcttcagga
     9481 aagaaatcca atagcgtata aggcggttca ccttctttac gtccgtccat atgccgagaa
     9541 taattttcaa ttcccgacgt gtagcccatt tctaacatca tttcaatatc gtaattggtt
     9601 cgctgttcta aacgttgagc ttccagaagt ttgttttcat ttcgcaagac agttaaacgc
     9661 tgttctaatt cctctttgat attcgctacg gcatgctcca tatgttcatc attcgtcaca
     9721 aagtgagtcg ccgggaagat tgctacgtgt tccgtttctc ctaaaacttc acctgtcagt
     9781 gcattgactt ctcggatacg ttcgacctca tctccaaaaa actccacacg taaggcacgt
     9841 tcatctcttg aagccggaaa aatctccacg acatcaccgc gcactcgaaa acgaccgcgc
     9901 tgaaaatcta tatcatttct ttcgaattgg atactgacta agtcacgaat caactgattg
     9961 cgatctagtt ctgctccctg tcgaatcgaa actacttgtt tttggtactc aaaaggcgag
    10021 cctaatccaa aaatacatga aacggatgca atgacgatca catcatttct ttctaataat
    10081 gaactagtag ccgaatgtcg taatttgtcg atctcatcat ttacacttga atctttttcg
    10141 atatacgtat cgcttgaagg cacataagct tcaggctgat aataatcata ataagaaaca
    10201 aaatattcaa cagcattgtt agggaaaaat tccttgaatt ccccgtacag ctgaccagcc
    10261 aatgttttgt tatgggcaat aatcagtgtt ggtttattca ctttctcaat aagattggaa
    10321 atcgtatatg ttttccctgt tccagtcgca cctaataaga tttgtgcttt tttcccgccg
    10381 actacgccat ccactaattg gttaatcgct tcaggctgat caccagccgg ttggtatttc
    10441 gagacaagct caaagtgtcg ggaggtatct ctctcaatca ttcttcgtcc tccttttcat
    10501 tcattccttt tcattttacg accaataaaa agaaaaggaa agcaaaggca ttccttacta
    10561 aaaaattata ccacaccgaa catactttcg ccactttttt atcttattat acatgttatg
    10621 ttttataaca taaaatgtta gaaactcgca tttttttcat tttttcgagc ttacagtctt
    10681 gtctgattca gcacttttcg cctataataa taaaatattg gaaatttagg aggttgatta
    10741 tgaaaagaaa gcatttattt cactcatttg ttttcttaat gacaggactt gtgacacttg
    10801 cgtttggtca tgttgcacaa gctgacaaca ctacaacggc agagtcaagc acaagcgaga
    10861 caatgcaaaa aatagagccc aaaaaagacg tatacgtaat tgccagtgac tctgcttttg
    10921 ctccatttga atttcaaaac gcaaagggcg agtacgaagg catcgatgta aaattgatgg
    10981 aaaacatcgc caaactgcaa ggatttaaca tccgaatgga ctaccgtggt ttctctgctg
    11041 ctttgcaagc tttggaatca ggacaagcag acggaatgat tgccggaatg actgtaacag
    11101 atgaacgaaa gaaaagctat gatttttctg attcttactt tgatagcggg atccaattag
    11161 ctgtcaaaaa aggagatact tcgatcaagt cttatgaaga tttgaaagga aaaacagtcg
    11221 gagcaaaagt tggtaccgaa agtgctgaat ttttaagcga ccatgaatct gaatatggct
    11281 attctatcaa atattttgat gctgctgatc aattgtacga cgggttaaaa gttggcgcaa
    11341 tcgatgcaat gatggatgat tatccggtaa tcggttatgc gatcaaacaa ggacaagaat
    11401 tggaaacacc gatcaaaaaa gaatctggcg gaaaatatgc atttgccgtt aaaaaaggac
    11461 aaaatcctga gcttcttaat atgttcaatg aaggattgaa agaattaaaa agaacaggcg
    11521 aatacgataa gattattaaa gaatatgtag cggatggttc agaaacttcc agtgagtctt
    11581 ccactgctga tgaatcaaca tttcttggac ttatccaaaa caactatcaa gcattgttga
    11641 aaggactttg gagaacaatt ctcctaactt tgatctcctt tgctttggct atagtaatcg
    11701 gtgtgatttt tggattattc agtgtagcac caatacgcgc tcttcgcaca attgctagta
    11761 tttatgtcga tatcatccga ggaattccaa tgatggttct tgcgttcttt attttctttg
    11821 ggttgcctgg tatcgttgga tttacgattc ctgacttcct tgctggtgtt atcaccttga
    11881 ctcttaacgc aagtgcttat atcgcagaga ttgttcgagg cgggatcaac gccgttcctg
    11941 tcggacaaat ggaagcttcc agaagtttag gtctttctta taatcggaca atgcaaaaaa
    12001 tcattttgcc gcaagcgata cgcatcatga tcccatcatt cgtcaatcag ttcgttatct
    12061 cattaaaaga cacaacgatc atttccgcaa tcggtgtagt cgaattgctg caaactggta
    12121 agatcattgt tgcacgtaca acacaaagta cgtatgttta cttgatcatt gcgatcatgt
    12181 acttgatttt gatcacagca ttgacaaaat tagcaaaagt tttagaaaag aaggtgaagt
    12241 aagatggcag aaaaaatctt agttgagcat ttagtcaaaa aatacggcga taacacagta
    12301 ttaaacgaca tcaatgtatc catcaacgaa ggagatgtcg tctgtatcat tggcccttca
    12361 ggttcaggaa aaagtacatt cttgcgttgc ttgaatcgtt tagaagaacc ttctgacggt
    12421 gaaattatta ttgacggcgc ccacttgatg gataaaagca cagatatcaa cttggtgaga
    12481 caacatatcg gaatggtatt ccaacacttt aacctattcc ctcatctttc tattttggaa
    12541 aacatcattt tagcaccgat ggacctgaag aaagaatcta gagaagaagc agagaaaaaa
    12601 gcaatcaaat tactgcagac agttggtcta gaagataaaa aagatatgta tcctgaaatg
    12661 ctttctggtg gacaaaaaca gcgtgtggct attgctcgtg ctctagcgat gaaccctgac
    12721 atcatgcttt ttgatgagcc tacttctgca ctcgatcctg aaatggtcgg tgatgtcttg
    12781 aacgtcatga aaaagttagc agaacaaggt atgacgatgg ttatcgttac gcacgaaatg
    12841 gggttcgcta aagaagtggc caatcgcgtc atgttcattg acgacggaaa ctttttagaa
    12901 gatggaacgc ctcaacaaat ctttgaaaat ccgcaaaatg aacggacaaa agatttcttg
    12961 aataaagtat taaatattta aaataaatta tatatgtctt tttgactatc tttttatatc
    13021 ttcttaaatg tccgcaaagc cttattttat aggctctacg ggcattttgc ttttgtggta
    13081 aacctcacat atctaggtct atcttcttat attttcacta tcaagcgtgg ttaaaatcgt
    13141 ggtaaatact tctatgcaat tagacgctga acctctgatt ttgcggtatc tatggacgca
    13201 tgagcatacc agttcattgt gatactgata tttgaatgcc ccatgatata ctgtaaatct
    13261 tttgggttca tgttcttgct tgccagtctt gtgcagaatg tatggcgtag cgtatgcggt
    13321 gtgatatgtg gcaatggatt gtccttgtgg tgcttgttgt atttctttac catacggata
    13381 aataaggcgt tataatcaat cgcaactttg ggcttgccct tatgattgac aaataggaag
    13441 ttgccccgtc cgtctatcac aaatggttct gcctttggac gttttttcat aacctgttga
    13501 aatgcctgta tcgtttcttt gcttaatggt acttgcctta ttccgctttt tgttttaggc
    13561 gtttcaatat aatagccctg ttccttgctc tttagtaact ggtggtcgat aatcacaact
    13621 tcattcttga aatcaatgtc ggctactgtc agtccgcaca gttccgaaat acgaagtcct
    13681 gtctttaaca gtatcagcac atcatcataa tgcttgtgat acacattgtc cgtcttgatg
    13741 aatgagagta aggcttgttc ctgttcctct gtcaatgcga ctttcttttt ggtatcattt
    13801 tctaggactt cactcaactt gaaatcaaag ggatttttcc tcacataatc atcttgaatg
    13861 gctatgtaga atgacgcttt taacgagcgt ttatggttgt taatggtatt gtaggaaaag
    13921 cctttgtctt tcatgcgtaa cgcccattcc ttagcgtcag agggtttgat tgtatcaatg
    13981 ctcctagcac ctaacttgtc ctctttcaat aaccgcatga gttgttcccg ttgtttctgt
    14041 gtgcttttct tcacatttgc cctctgtgcg ttctgtttgg cataaagttg gcaaagcgtc
    14101 attttcttgc cagtgctgtc gataccgtcc tcaatatccc gtcttatctg ctgttccagt
    14161 tctcgaagtg agggtttttc ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc
    14221 caagcataca catattttgt gtttccaaat gcgtctacat atttatataa gtattttctg
    14281 tctgttcgtt ggctctctcc agtatgcagg atacgacctt tgttatcccg tcttttttct
    14341 ttcatggtgt ctgctccttt ccttgttgga aagagccttg atatgacttg tgttcatcat
    14401 aacacataca aggctcattt gcattagata gcgtccaatt tatcaatgac ctgttcaaac
    14461 tgtcggcgtt taatctgtat gcggttgcca ttcataatga gccagccagc gtccttgttt
    14521 tcctctgcca atcgtcttaa cttgttttct ccgatacgga aatactttga cgcttcttca
    14581 atggtaaggg tgtatttttc ccatacggga atatcctgtg cattactcat aaaacacccc
    14641 ctcactttcg attacgtctg ctatgtccgt acacatctgt tccacaagca tttcctgctt
    14701 ttcagagagg tgggtacatt catcaatcat tttcatgtag tttgagcctg tcagcctgtc
    14761 gatttcctct atcatcacga ttgacggcat gacctgtctt tccagccatt gcagagtgcg
    14821 ttccatattg acaggtacgg ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat
    14881 ctcccacagc ggatagtcgg gaaattgcac aaaatcagta ataaggtaaa acaccagccc
    14941 atgagggtta tatgtcaata acaattcctc aactgcctgt actgcttttg taccacgcag
    15001 acgaatttca aaacgattga taatatccgt atgtttcttt ttggttgcct gttccttttg
    15061 tttttcatac aggcagaagt attttgtgct tgcctttgaa ccgatataaa gggtgcttgc
    15121 taaattacgg ttttttccgc ttagttttcc accctgcaca ttttcataat ttttacagcg
    15181 acaatccgca ccgccatttc tgtatttttc agacaaaacg ggaatatcca acagaccaca
    15241 catatcattg attgccaagt caaaacgtcg gataacaccg tcacagtcaa gacagcggtt
    15301 taaaaatgaa taccaatctc tgttctgtgc ctgcaagaca tattccatgt tgcgtgagcc
    15361 tgcccctttt aattccaaga atacgcccat gtgttcgtca tcagaagcca tgaccttgat
    15421 ttctccgtag gcgtaatgct ctttatagcc ataataccca tagtcataat ggataaaata
    15481 atctgctttc atgccaagca cctttcggat aatttccaat gcgtcagtcg ttggataacg
    15541 gacagacaga taatcaacga gcagataaaa aggcttatcg cagatataat aattcagaca
    15601 acgcaggata atattttgtg tttccttgct ggcgttggct tttccgttct caaagcggtt
    15661 gatactatat cttgatacat gggtaagact tgcaagttct ccttgtgaaa gaccgcttgc
    15721 aagacgtttg ttcttcattt caagtgcaaa ttctaaattg ttcattgtat tcctccaatc
    15781 ccacaatagc cttgaaatac ctgtaaacct ctattcaaaa agtgtgatat gcactatctt
    15841 cttaaaactt ctcattacta ctcacaactt tgggattgta tggtttttga cttgtctttt
    15901 gtgaaatagt gcccccctgt tagataacgg gggcacagac aaggctcgca ggctcgccag
    15961 ccgacaaagc atttcttccc cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc
    16021 cgccaccgtg tcagaaatac tttgtcagca agttctgttt tgccttttct aacaagttcc
    16081 atttcaaccg atattcatat ctacggattt aatcagatag gtatttcttt ttgaatgttt
    16141 ttatatttca ttctattgtg cttattcgat tacaagaaca tagtaattct tatatgctta
    16201 tttgtcaata tctttttttg cctttacaaa tcatattaag catgatagac ttataatagc
    16261 gaagatataa cgagatatgg agggttatgc aatgaaaata agccatttag gaaataatat
    16321 acagaccata agaaaattta ggggaatgaa acaacaggaa cttgcggaca aaatcggtat
    16381 caatatgcag agcctttcca agattgaaag agggttgaac tatcctgcct atgaaacgct
    16441 ggaaaagata atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa
    16501 gtatgtcaat caatctgaaa aggaagtctg tcagttttta agagccgaag aacgtctaaa
    16561 tgcagaactg aaacatggac actatgataa cttctttgac agcgaggaag aatggctgga
    16621 atatgagtta gaaaagttac gggaatatat tacagactat atcaacggga aaagcattga
    16681 agcgtccgac ctttacccaa tcaaagaatt tatccagcat ttgaagtttc aaaaattatt
    16741 agaccgctat gatgatttat acagtatgga tatgtttggg gaaagcatag agggacataa
    16801 gtataagaca ccttatcaag tcgtgaaaat gataaacccg aactcaaaag aagatatgga
    16861 actgttacga gaagtattaa ggaataacaa ttttgatgat gaggacgagt aacttttttc
    16921 ctcttgcgag aagatacagc aaacaaaaag cccgatcaag agtgcaaagc accaccaatg
    16981 gtggcaaagc tcttgacagg gctttttctt ttctgttgtg ggcaatcaag aggaagaaag
    17041 ggataaaatc tgatttgtgg taaatcaggc aaaggcaacg caggtatctc aatttgattt
    17101 ggtacttgaa aagaacggga gtaattggaa gattatagaa taacaaatat tggtacatta
    17161 ttacagctat tttgtaatca cgtactctct ttgataaaaa attggagatt cctttacaaa
    17221 tatgctctta cgtgctatta tttaagtatc tatttaaaag gagttaataa atatgcggca
    17281 aggtattctt aaataaactg tcaatttgat agtgggaaca aataattgga tgtccttttt
    17341 taggagggct tagttttttg tacccagttt aagaatacct ttatcatgtg attctaaagt
    17401 atccggagaa tatctgtatg ctttgtatgc ctatggttat gcataaaaat cccagtgata
    17461 aaagtattta tcactgggat ttttatgccc ttttgggttt ttgaatggag gaaaatcaca
    17521 tgaaaattat taatattgga gttttagctc atgttgatgc aggaaaaact accttaacag
    17581 aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa
    17641 cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa
    17701 cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt
    17761 tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg
    17821 caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga
    17881 ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt
    17941 atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt
    18001 atcctaatat gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg
    18061 gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg
    18121 aacaagagga aagcataaga tttcagaatt gttctctgtt ccctctttat catggaagtg
    18181 caaaaagtaa tatagggatt gataacctta tagaagttat tactaataaa ttttattcat
    18241 caacacatcg aggtccgtct gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa
    18301 aaagacaacg tcttgcatat atacgccttt atagtggagt actacattta cgagattcgg
    18361 tcagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg
    18421 aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt
    18481 tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa
    18541 atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt
    18601 tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt
    18661 ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg
    18721 cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata
    18781 tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt
    18841 tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg
    18901 agagctcggt ttctcttgga tacttaaatc aatcgtttca aaatgcagtt atggagggga
    18961 tacgctatgg ctgtgaacaa ggattgtatg gttggaatgt gacggactgt aaaatctgtt
    19021 ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg atgcttgctc
    19081 ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag ccatatctta
    19141 gttttaaaat ttatgcgcca caggaatatc tttcacgagc atacaacgat gctcctaaat
    19201 attgtgcgaa catcgtagac actcaattga aaaataatga ggtcattctt agtggagaaa
    19261 tccctgctcg gtgtattcaa gaatatcgta gtgatttaac tttctttaca aatggacgta
    19321 gtgtttgttt aacagagtta aaagggtacc atgttactac cggtgaacct gtttgccagc
    19381 cccgtcgtcc aaatagtcgg atagataaag tacgatatat gttcaataaa ataacttagt
    19441 gtattttatg ttgttatata aatatggttt cttgttaaat aagatgaaat attttttaat
    19501 aaagatttga attaaagtgt aaaggaggag atagttatta taaactacaa gtggatattg
    19561 tgtcctgtat gtggaaataa aacacgatta aagataaggg aagatactga attaaaaaaa
    19621 ttccccctct attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa
    19681 gtaactgtga ttacagagcc agacgcaaag acgcagagcc gataaaatga gattaataca
    19741 atctcatttt atcggctctt tccgttatgt atggattctt ttaattagtc ttcgatgttt
    19801 cttgcttcgt tgataccgct ggctaaagat tccattaagg atagttcttt gtctgtaaag
    19861 ctatccatgt atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt attagcaggt
    19921 aagaaaaatt catcaacgga aacatgaagt aacgatacaa ggtcataaag aacttgtatg
    19981 ctggggtgtt gccctttatt ttcaatatta gttaagtacc gtgggtcaat ttcaatcaat
    20041 gctcccactt gttcacgagt taaacctcgt ttcaatcgag cttctttaat ggctaaacca
    20101 aaggctctaa aatcatattt atcttctttt ttacgcatag tagaccacct ctatacattt
    20161 tattgttcct actgaattaa aaacaggtat agaaaaacgt gttatatggt ttataggttt
    20221 atatttaata aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg gtagtgctat
    20281 ttacgctgtt aaaatattgt atattacttc caaatggcgg tttgttggag gtcaacgtcg
    20341 ccatgaagta catcatatac aataaatttc cttacattgg gttcttgtca aaaaaagtcg
    20401 tctatctgca atagataagt acgtccacca atgtggtttt ataaatcata tagatagaat
    20461 aacagaagca tgtaaacaga gaaataaatc tgtttatatg cttttttggc tattcagaac
    20521 ttttttacaa agtttattta tcagtaatgc aacaaatccc cctttcacat tgggactaag
    20581 agtgaaagga gataaacgag caaggctcac ttcctttcct agacagaaag ggggtgagat
    20641 tatgaggaca tcttctttcg agcatatcgt cagattacag ttcaattctt taatgctgat
    20701 tgtcattaag aacaaagtga aaagtcgaaa cagacagctt gcaagacatt ctaaacgtga
    20761 agtattgttt tgtgaaatag ccgaaacgaa acaagttgaa cgtggaacga ttgatacctg
    20821 tttttatgat tgtgtatcat ttaaagtctt gcactttaca atttatgtat ctgatgaaac
    20881 acttggtaca gttttacata gactatcaga aaaacagcgt agtgcgattt tattacgtta
    20941 ttttcaagga atgaacgaca gagaaatttc agaactgtac catgtatcac gttctgctat
    21001 ttgcagtaga agaactagag gactaaggaa gttaaaaatg cttttgagcg aaaggaagta
    21061 aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg
    21121 cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt
    21181 tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa
    21241 ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg
    21301 tataaaaaaa tcgggagata cacagtttcg tgttatctcc cttagtagtt ttacggtcat
    21361 atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa
    21421 aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa atttaataaa
    21481 aaccaaaaat gcacggtcgc catgaaggga ccgtgtattt ttatcttctt attttctcac
    21541 ctaatcgtat cagccgcatc gcttgttttc ctgcacaaac aagcaatcgc tctacctgtt
    21601 ccatcgcata agaaagtacc atcggttcat tgatgatatc aagtacatag tcgataccac
    21661 cctcacttaa ttgagcttga tttttcttct ccatattatg tagttgtggg acaactttct
    21721 gacaatcagc aattgtgtca agaacggatc aatcctttat tggagttgct cgaaagggtc
    21781 ttctcttatt acggacaggc tttgctcact gtacatagac aacaaatcat tatcctagtc
    21841 aatcggattt ccagagcctc attgctatca ttgcttgata aaatccagac aaagtttaat
    21901 aaaatgtatc agttacagct aaactttgga attgggtcgc tttgttatac agagcaagag
    21961 acaccgcaat ctttccttca tgccaaacaa gtttgcgaat ggatagcttt ccatcaatct
    22021 gtgaacgaga tccgcttttt tgaagattta gacctcggga tcgttttacc agcaatcccg
    22081 tcagatcaac gaacattata tgtaaaacgt attttgaaaa gcctgacaga agaagaagta
    22141 catttgttca agaagacttt ggcatgcttc agtaaaaata acggaagcat caaaaattgc
    22201 tcagaagaat tatttattca taaaaacacc ttgcaatatc gattgaacaa gttccattct
    22261 ctcactggtt atacccctag aaactatgac gattatcata ttctaaagct ggcttttttg
    22321 ttggtacaaa catgacaact acaaaaaaac caggcgaaca tatgattgtt cgtctggttt
    22381 ttaattatta atgacttagc gtttttacta gaaaaactac ttccgctttt tcccgccaaa
    22441 agcatcttta aacttatcga agaaaccgtc ttcttgctgt tcttcaactt tgatgccgcc
    22501 agcttcagcg aatgcacgta atgcttctct ttgttgttca tttagatttt tcggtgtgat
    22561 cagttttact ttgacatgtt ggtcgcctgt tgcaccacca cgtaacttcg gagcgccttt
    22621 tccacgtaaa cggaagttcg tacctgtctg tgttccagca gggatcttca atttcacatc
    22681 tccatggaca gttggtactt ggacttcatc ccccaaagct gcttgtacaa aacttaaagg
    22741 caattcatag taaatttcag aaccttcacg atcaaagata tcactttctt ctacacggaa
    22801 gaccacatac aaatcaccaa atggtccgcc gttttctcca gcttctcctt gaccagctaa
    22861 acgcatttgt tgtccttctt ctacaccagc tggtacattc actttaactg aatgtgcttt
    22921 cttctcatgt cctgagccat gacaggttgg acaaggttct ttgatttctt tccctgtccc
    22981 atgacaaaca tcacaagttt gacggctcat cactcgtcca agtggtgttt ggcgttctac
    23041 attaatcgta cctgcaccat gacatttatg acaagtagtt ggttgtgtgc ctggttttgc
    23101 accgttccca ccacaagtat gacagatttc ttcccgatta tacttgattt ctttttctac
    23161 accaaaaatc gcttcttcaa aagacaagtc gaccgtatat tgcaaatcag caccttgacg
    23221 aggcgcattt ggatcgactg accgaccgcc tcctccaaag aaagagtcga agatatcttc
    23281 aaaaccaccg aagccgccac cagagaagcc gccgaatcca ccaaatccgc caccagctcc
    23341 accagcaccg taatttggat ctgtgcctgc atgcccgtat tggtcatatg cagcgcgttt
    23401 ttgcgggtca cttaaaatct catatgcttc tgatacttct ttgaattttt cttccgcatc
    23461 aggttcttta ttgatgtcag gatggtattt tttcgaaagc ttacgataag ctttctttat
    23521 ttcatcttct gatgctcctt ttgagagccc taagacttca taataatcac gttttgttgc
    23581 cataggcttt cctcctattg gttctcatca ttttcacgtc gcttgttatc atatcatatt
    23641 ttgtttgaaa caaaaaccga tacttcaaac tatttttcaa aagaaaaagc caaagtagca
    23701 actttggcct tctctcgaaa ttcagtgaac tgattattta tcgtcgccat cgacttcttc
    23761 aaaatcagca tcaacgacat catctgctcc gccttgagct gcttctgggt tttcttgagc
    23821 ttgttgttgt gccgcttgtt cgtatagttt aacagtcaag ttttgaacga tttcgttcaa
    23881 tgcatcgcgt ttagttttca tttcttcgat gttgtttgct tcaacagcgg ctttcaattc
    23941 atcgcgagct gtttctgctt ttttcacttc ttcttcgtcc actttgcctt ctaattcttt
    24001 caatgttttg tccacagaga atagcaatgc atctacatca ttacgtaggt caacttcctc
    24061 tttacgagct ttatctgctt ctgcatttgc ttctgcatct tttaccatac gttcgatttc
    24121 ttcgtctgtt agacctgaag aagatttgat cgtgattttt tgttcttttt gtgtacctag
    24181 atctttcgca gatacattta caatcccgtt tttatcaata tcgaatgtta cttcgatttg
    24241 tggaatacca cgaggagctg ctggaatatc tgtcaattgg aatcttccta gagttttgtt
    24301 gtcagcagcc attgggcgtt caccttgtaa tacatggatg tctactgctg gttgattatc
    24361 tgccgcagtt gagaatactt gtgatttact tgttggaatc gttgtattgc ggtcaatcaa
    24421 tttagtgaac actccaccca ttgtttcgat acctaatgac aatggtgtaa cgtcaagtaa
    24481 aacaacatct ttcacatcac cagtgatcac gccaccttgg atagcagcac ccatagcaac
    24541 tacttcatca gggttaactg atttgtttgg ttcttttcct gtttctttac gtactgcttc
    24601 tacaactgca gggatacgag tagatccacc gactaaaata acttcgtcga tttctgattg
    24661 tgataaacct gcatctttta gcgcttgacg tactggtact tttgtacgtt ctactaagtc
    24721 agctgttaat tcatcaaatt ttgcacgtgt caatgtcatt tccaaatgca atggtccaga
    24781 ttctcctgct gtgataaatg gcaagctgat ttgtgtgctt gatacgccag ataaatcttt
    24841 tttcgctttt tcagcagcgt ctttcaagcg ttgtaaagcc attttgtctt gagacaagtc
    24901 aatgctgttt tcttttttga attctgctac catgtagtcg atgattttgt tatcaaagtc
    24961 gtccccacct agatggttgt cgccagctgt tgacaataca tcaaagacac cgtctcctag
    25021 ttcaaggata gatacgtcaa aagtaccgcc accaaggtcg aatactaaga ttttttcgtc
    25081 gcgatctgtt ttatctaaac cataggcaag agcagctgca gttggttcat tgacgatacg
    25141 ttctacttct agaccagcga tcttaccagc atcttttgtt gcttgacgtt gtgcatcatt
    25201 aaagtaagct ggcacagtga taactgcttt ttcgactttt tcacctaaat agtcttctgc
    25261 gaatcctttg atatattgaa ggatcattgc tgagatttct tgtggtgtat atgatttacc
    25321 ttctacatct actttgtaac ctgcttcacc gatatggcgt ttgattgatg aaatcgtatt
    25381 tgggtttgtc acagcttgac gttttgcaac ttcccctact tggatttctc catttttgaa
    25441 tgatacaaca gaaggtgttg tacggtttcc ttctgggtta gcgatgattt ttgcttcgtt
    25501 tccttccaat actgctactg cagagttagt cgtacctaag tcaataccga taattttact
    25561 cataattaac gtctccttct ttaattgaaa gtgtaagttt tatatccgca caattattgt
    25621 gcaacaatga ccatggttgg tcttaacacg cgatcatgta atttgtagcc ttcttgtagt
    25681 acttctacaa tcgtatcggc tggtgtatct tcagtcgctg gaacagtttg tactgcttga
    25741 tgaagattag gatcaaaggc ttctccttta gcagggattt tttcaattcc ttcttcttcc
    25801 agtgcgttac gcaaactttc caagaccatt tccacgcctt ttttcaaacc tgccccttga
    25861 tcatctgaca cttctgtggc taatgcacgt tccaggttgt caattgatgg aagtaatttt
    25921 ttggctaaat cttgtgaacg gtatttttgt aattgctcac gctcattttt tcctcgattt
    25981 gccatatttg cgatttcagc acgagcccgc aagtatttat cttccatttc ttctaactca
    26041 gcttttaatt tcgctgtttc gaattcttcc gcttcagctt ctgaaatacc cgaatcttct
    26101 gcagcttcga catcgatttc cggttctggc tgagcatctg tcatttcttt atctagttct
    26161 tcttgatttt ctttcacgat acagtcttcc tttcattatt cagtcagctg tttgattgct
    26221 cttactaaga aagagagaac gatcagctgc aaatagcttc gttattcaca ttacgagttt
    26281 gacaagtcaa gcgaacggta gtaatcagca agtttagctg ctagttctct tcgataaaca
    26341 tcgactagac cgaacatccg agaataaggc atactagtag gccctaacaa ggctattgta
    26401 cctcttccat gaccatctat ctcgtaactt gcttgaatca tactcatatt ctgcaataaa
    26461 tcattgccta attcagcacc aatcttgata tgaatattgc tatccatcgg agtgatcatt
    26521 tgtgtaagtc cgtctgagtc tttcataaaa gaaaacatcg atttgaactg atcaagatct
    26581 tgacggggct cataatccaa gagattcatt tgtccactga cataaacctt atcttcaaaa
    26641 acatgaccaa gaacggtgtc aaataactga gacatccctt ctgtcgtttg gaaatattta
    26701 tgaagaatca tcggaatctc cgtacgaagt ttatggtaca ccgtcatcag aggatctccg
    26761 actagacggt cgtttacaat ctgtaccatt ttttctagtt cctgacttcc catatattcg
    26821 ggaagagtga acacttggct ttctacattc cccatatctg ttacgacgat agcaataatc
    26881 tgtcgattat tcaagggtac gatacgaaag ccagtcagtc ggcggtcttt tatttcaggt
    26941 cccaatgaaa aagtcgtata gcttgtcagc tcagataata tctcagcaga ttgcttaata
    27001 atctcattga tttcatgaaa ttctctgcct agagactgac gaatcagcag caaatcatct
    27061 tctgctacgc gagcaggttt taacaaatga tccacgtaat aacgatagcc taaagcagat
    27121 ggaatccgcc cagaagaaga gtgagtcttc aacagcaatc cttcatcttc taatgctttc
    27181 atatcattgc gaatcgttgc tgagctggcc ttgactccct cagacatcaa tgttttggaa
    27241 ccaacgggca cgccactact cgtatagtgt tggatgatca gacgtaaaat gtcgttttgt
    27301 cgctttgtta acatcttcct cacctctcat tagcactcat atattctaag tgctaatcca
    27361 cttataaata tagcaagaag cccttctcct gtcaagagaa agactccatt ttttagcact
    27421 ctaaacacaa gagtgctaat tctaaataat tgatgtaaat ccagttatat caacgtttca
    27481 atagtccttt attgtatttt tgaacttttt ttatttaaat tcagataaaa aacaaatatt
    27541 ctttaaacta tttttcaaac aaaaagcgct caaacacatt gttccccaaa aaggtccctc
    27601 ttggtgtcaa ataaacacgg tcttcttcat taacaagtaa cccttcttgc tctaattccc
    27661 ttagcacatc accgtacacc gattcaattg ttttttgata gcgatcatag aaatgttttt
    27721 tagtaatccc tgtttttttc ttagtcctaa gaacatttct tcttctattt gattttttcg
    27781 tgtcaatttt tcttcttcca ataccgggcg ttcatttgct cgtaatgggc gtaaatagtg
    27841 ttggattggt cctttgttgc ggtaacgaat atctccaaga taaccgctag caccagctcc
    27901 aaacccaaaa tagtggtcat tgttccagta catgaggtta tgttgagatt catgtccgga
    27961 gagcccaaag ttacttattt catattggtg acgtccatgc ttttccattg cttcaatcgt
    28021 ctcttcaaac attctggttt ccgtttcctg atcaggcagt tcgagtcttc cttgtcgtac
    28081 ccagttcatg aacattgttt tgttttcgag gatcaatgag tacaaagaat agtgaggcag
    28141 atccaaagcc aaagcttttt ccaaagtatc cagatatcct tctagtgttt gtcccggtaa
    28201 tgcataaatc aaatcgatgc ttacattggt aaaattttct gcttctaaaa attgcatagt
    28261 ttgatacaca tcttctgcgg tgtgtttacg tccaattttt ttcaataagc gattatcaaa
    28321 tgtctgcact cccatggata aacgattgac gccatagttt ttcattactt gaagtttttc
    28381 acgggtcaaa tcacctggat tcgcttctac agtaaactca ttaccatttt gaaaaggcag
    28441 gatctctcta gcacctgata acagtcgatc aagttgttta gcagataaag aagttggtgt
    28501 cccgccccct acgtaaagtg tttctaaatt cgctgtcgga tgactttcta gcattatttt
    28561 catttcattc agcagcattt ccacatactc atctactggc tgcccttcta aaaaaacttt
    28621 attaaaatca cagtaatagc agatatgctc gcaaaatggg atatgaatgt acgcagaaac
    28681 cttgttttct gcgggattta ttgtttctat cattgaaatt ctttctcctt acgtcgaaaa
    28741 gtcgtgcctg ttttcgacct tcttaaaatc gcgttcaagc accaactcat tgaaataagc
    28801 cgcatattta gaatcaagga aacctagctc taaaaatgct tgcttattgt catcggagct
    28861 acacggtgct tttacagcct ctcgataaac ggtaatcgca aagctgacct tcggcattaa
    28921 gtcaaaatct gcaaaaatat gaaaagctat tttcacagat tcctccttaa gtgctcagac
    28981 cacaacgctt tgcttacaac ctctcatata cgcatactaa acgtatttgg cgaacaaatc
    29041 cactaaaaaa tccaagaatc gacttcttgg atgatttttt ctttcattaa actggttacc
    29101 tagttatata tctctagggt tgttttcatg tctgagtgcc cgatatgatt tatttttcgt
    29161 ttctataatt actttttgcc ttccagtttc ttttaatatt gtactcaagc aagtgggaat
    29221 tggttctttt gtcatccatg tatgcaaaat gttataatgc tggtaaacaa aaatgattac
    29281 ctgtcatttt tatttagttc gtatttaatt aagttgacta tctgtatact ctgtctaaaa
    29341 cttaagaatt gttgttttcg ctgtcgctac ccaattttaa tgaatgtctg taggttaaaa
    29401 ctgttgtcct tgttatagat agtaaaggag agcttgaaaa tgaaaactgc gattacgttt
    29461 ctattagagg aatatgccga ttgggaaggt gcttatatta ctagcacact aaaccaaaat
    29521 gagaaatggc aaaacaaaac tgcttctatc caatcagagg tcacttccat tggaggatta
    29581 aaaacaaaaa tcgattactt aatcgaacaa atacctgaaa aagttgacct ttttattatg
    29641 atcggtggca attcatggga cataaaaaat catcatttat atcaaataat cgataaattg
    29701 ttaaaaacag acgtaccagt agctgctatt tgtggggcag ttgattattt agctcagagc
    29761 ggattattgc aaggttacaa acacaccgga aatgcgcaat ttccttggtt agaatcggaa
    29821 aattatcaga ataaagaaga ttttatagaa caacaagctg tgaaagatcg taatttgatt
    29881 actgcgaatg gaacagctcc tatagaattc acagacttga ttcttcaagc agtccatttt
    29941 gaaacagaag aaataagaga aaaaacgatt cgtctataca aacttggatt ctatgattac
    30001 tataaaaagt acggagatcc tttctcctaa tcattctctt caattaaata ctccttatca
    30061 atagtgtgac aagtaatttt actgatgatt atttgtcaca ctttttctga catttatcaa
    30121 agaagataca ctctatttat attcatagac aaggttttac ggattcaaaa attcaggatt
    30181 atgctatact ggtattaaaa aaataatgag gtattttata tgaaaaagtt aggttttatt
    30241 ttattgttgg tctctttatt ccttgtagga tgtacgtctc ctagtaaaga aaatagtgat
    30301 ccatctgcga ccacagacaa aagttcaatc agacaaacca gttcagctga aaaatcaagt
    30361 actcagaaat cagcttctac tgaaacgaat acatcttcaa caagcagtaa agtctcagag
    30421 cccaatactt cttctacatc tagtagtaca gttgagacaa atatttcttc ttcaacaata
    30481 gacacaacct cctcacagtc ttctcagtca atagatccgt tagcaggata ttctgatcta
    30541 caaattgagt acgcacgtgt ttggctagct attagaggta atacttttag agatacatcg
    30601 ggtgatttcg aattacatgt tattcattat cctgcaggaa cacctatcgc cccttatgat
    30661 gcagggagtg cagtctatcc gaaagatgtg gttatgctca ctgggaaata cagttaccag
    30721 ggagttttag tgtattcaag taaccatgac gggacaatca ctagttatcc agttccttca
    30781 cattggcaaa tgcctgcaga tcaaacatct tatccagcgt tcattagaga aacaactcaa
    30841 gaaatcattg ataatgcatc tgtagtagct attcctacag gaaatcccaa tgacgtgaaa
    30901 cagttaatcg atgtgacagt tatagataat tagactttct aatatgttat acaaaaagaa
    30961 gctgacagc
//
LOCUS       DANKAY010000024        30459 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000024 DANKAY010000000
VERSION     DANKAY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(451..1761)
                     /locus_tag="PWY98_001076"
     CDS             complement(451..1761)
                     /locus_tag="PWY98_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2036839.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="PWY98_001077"
     CDS             complement(1773..3131)
                     /locus_tag="PWY98_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2036840.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="PWY98_001078"
     CDS             complement(3333..4901)
                     /locus_tag="PWY98_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2036841.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="PWY98_001079"
     CDS             complement(4905..5315)
                     /locus_tag="PWY98_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2036842.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="PWY98_001080"
     CDS             complement(5312..6235)
                     /locus_tag="PWY98_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2036843.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="PWY98_001081"
     CDS             complement(6232..7110)
                     /locus_tag="PWY98_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2036844.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="PWY98_001082"
     CDS             complement(7097..8434)
                     /locus_tag="PWY98_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2036845.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="PWY98_001083"
     CDS             complement(8437..9540)
                     /locus_tag="PWY98_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2036846.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="PWY98_001084"
     CDS             complement(9614..11122)
                     /locus_tag="PWY98_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2036847.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="PWY98_001085"
     CDS             complement(11088..11780)
                     /locus_tag="PWY98_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2036848.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="PWY98_001086"
     CDS             complement(11802..12776)
                     /locus_tag="PWY98_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2036849.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="PWY98_001087"
     CDS             13076..13948
                     /locus_tag="PWY98_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2036850.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="PWY98_001088"
     CDS             complement(14406..16604)
                     /locus_tag="PWY98_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036851.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="PWY98_001089"
     CDS             16836..17828
                     /locus_tag="PWY98_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2036852.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="PWY98_001090"
     CDS             17888..19309
                     /locus_tag="PWY98_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2036853.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="PWY98_001091"
     CDS             19302..20801
                     /locus_tag="PWY98_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2036854.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="PWY98_001092"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="PWY98_001092"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2036855.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="PWY98_001093"
     CDS             22393..23076
                     /locus_tag="PWY98_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2036856.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="PWY98_001094"
     CDS             23078..23914
                     /locus_tag="PWY98_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2036857.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="PWY98_001095"
     CDS             23925..25679
                     /locus_tag="PWY98_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036858.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="PWY98_001096"
     CDS             complement(25955..26296)
                     /locus_tag="PWY98_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2036859.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="PWY98_001097"
     CDS             complement(26364..28535)
                     /locus_tag="PWY98_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2036860.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28704..29603
                     /locus_tag="PWY98_001098"
     CDS             28704..29603
                     /locus_tag="PWY98_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2036861.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29674..>30459)
                     /locus_tag="PWY98_001099"
     CDS             complement(29674..>30459)
                     /locus_tag="PWY98_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2036862.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9695 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaattt
//
LOCUS       DANKAY010000025        29348 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000025 DANKAY010000000
VERSION     DANKAY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     misc_binding    20..365
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            410..1051
                     /locus_tag="PWY98_001100"
     CDS             410..1051
                     /locus_tag="PWY98_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2036863.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1070..1705
                     /locus_tag="PWY98_001101"
     CDS             1070..1705
                     /locus_tag="PWY98_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2036864.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            1723..2538
                     /locus_tag="PWY98_001102"
     CDS             1723..2538
                     /locus_tag="PWY98_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2036865.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            2857..4293
                     /locus_tag="PWY98_001103"
     CDS             2857..4293
                     /locus_tag="PWY98_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2036866.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            4482..6929
                     /locus_tag="PWY98_001104"
     CDS             4482..6929
                     /locus_tag="PWY98_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2036867.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7159..8226
                     /gene="rlmN"
                     /locus_tag="PWY98_001105"
     CDS             7159..8226
                     /gene="rlmN"
                     /locus_tag="PWY98_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2036868.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(8419..9522)
                     /locus_tag="PWY98_001106"
     CDS             complement(8419..9522)
                     /locus_tag="PWY98_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2036869.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10075..10623
                     /gene="aac(6')"
                     /locus_tag="PWY98_001107"
     CDS             10075..10623
                     /gene="aac(6')"
                     /locus_tag="PWY98_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2036870.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11169..12830
                     /locus_tag="PWY98_001108"
     CDS             11169..12830
                     /locus_tag="PWY98_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2036871.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(12900..13247)
                     /locus_tag="PWY98_001109"
     CDS             complement(12900..13247)
                     /locus_tag="PWY98_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2036872.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            13331..14272
                     /locus_tag="PWY98_001110"
     CDS             13331..14272
                     /locus_tag="PWY98_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036873.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            14273..15316
                     /locus_tag="PWY98_001111"
     CDS             14273..15316
                     /locus_tag="PWY98_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2036874.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            15322..16392
                     /locus_tag="PWY98_001112"
     CDS             15322..16392
                     /locus_tag="PWY98_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2036875.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            16382..17335
                     /locus_tag="PWY98_001113"
     CDS             16382..17335
                     /locus_tag="PWY98_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2036876.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            17347..18357
                     /locus_tag="PWY98_001114"
     CDS             17347..18357
                     /locus_tag="PWY98_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2036877.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(18642..19463)
                     /locus_tag="PWY98_001115"
     CDS             complement(18642..19463)
                     /locus_tag="PWY98_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2036878.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(19560..19838)
                     /locus_tag="PWY98_001116"
     CDS             complement(19560..19838)
                     /locus_tag="PWY98_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036879.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20106..22115
                     /gene="metG"
                     /locus_tag="PWY98_001117"
     CDS             20106..22115
                     /gene="metG"
                     /locus_tag="PWY98_001117"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2036880.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(22594..23181)
                     /locus_tag="PWY98_001118"
     CDS             complement(22594..23181)
                     /locus_tag="PWY98_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036881.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            23356..24678
                     /locus_tag="PWY98_001119"
     CDS             23356..24678
                     /locus_tag="PWY98_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2036882.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            24833..25609
                     /locus_tag="PWY98_001120"
     CDS             24833..25609
                     /locus_tag="PWY98_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2036883.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            25602..26171
                     /gene="rnmV"
                     /locus_tag="PWY98_001121"
     CDS             25602..26171
                     /gene="rnmV"
                     /locus_tag="PWY98_001121"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2036884.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26164..27048
                     /gene="rsmA"
                     /locus_tag="PWY98_001122"
     CDS             26164..27048
                     /gene="rsmA"
                     /locus_tag="PWY98_001122"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2036885.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            27499..28128
                     /locus_tag="PWY98_001123"
     CDS             27499..28128
                     /locus_tag="PWY98_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036886.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(28252..29346)
                     /gene="ugpC"
                     /locus_tag="PWY98_001124"
     CDS             complement(28252..29346)
                     /gene="ugpC"
                     /locus_tag="PWY98_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2036887.1"
                     /translation="MKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGKSTTLR
                     MIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGLKLRKY
                     DKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMDEPLSN
                     LDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIGTPKEV
                     YDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGYEGKEV
                     IFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKVDARDF
                     HKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         9663 a         5167 c         6134 g         8384 t
ORIGIN      
        1 attacacgta aagttaaaac aaatgacatc gaaaagaaga gtagccgtta ggtgttttta
       61 gagagtctct ggttggtgga aagaggcagc agttaaccgt gaaacacatc ttggagtcga
      121 ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag
      181 atgaccagat aaggctggct tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga
      241 ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt tcatctgaac ggcaacgact
      301 gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg gaaccacgta taatacgtcc
      361 tcttggcatt tgccaagagg atttttttat tttaaaggag gaaaagaaga tggagtattt
      421 gattgaagtt atgccgcaat tattagtagg agcactcaca acattgaaat tatttttatt
      481 taccttgatt ggctcattac cattagggat tgttttagca ttaggattgt ctttgcattt
      541 taagccgcta cgctacttgc ttcaaacata tgtatgggtt atgcggggaa caccgctgtt
      601 gttgcaactg atttttattt tttatgggtt gccgacgatt gggatcgttt ttgatcgttt
      661 cgaagcagca ctcattgcct ttatcttaaa ctatgcagct tattttgcag agatctttcg
      721 tggagggata caatcgattc ctcaaggtca atatgaagca gcgcaagttt tgcaattaag
      781 tccctttcaa acggtgacga aaatcgtcat cccacaagtg acaaagattg ttttaccttc
      841 tgtagggaat gaagttatca acttggtcaa agattcttcc ttgatctata tattaggttt
      901 aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc gacgcgactt tactgccttt
      961 gctaggcgtg gcaatcatct atcttacatt gactggtatt ctaacaatca taatgaaaca
     1021 agttgaagga aaattaaatt attaccgcta actgatagga ggaagaaata tgattgaatt
     1081 gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc aaagatttga atctaatcat
     1141 tccagatggg aaaacattag ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt
     1201 acgtatcttg gctggcttag aaagttctga cagcggagaa ttttatttag acggtgtacc
     1261 gttcaaccca ttcgagctta aaatgaaaga acaagttgta ggagtcgtat tccaagactt
     1321 ccagctattt ccacatctga atatttttga aaacattacc ttagcaccaa aaatggtatt
     1381 gaaacagaca aaagaagaat acaccaaaaa agcagaagaa ttagcggctt cattagggat
     1441 tagtgattta ttgaatatct atccttatca gctttctggc ggacaaaaac agcgggtagc
     1501 tattgccaga gcattggcta tgaatccacg ggtattggct tatgatgaac caacaagtgc
     1561 tttagatcct gaattacgcc agcaagtcga gcgtttgatc ctatcattga aagaagatgg
     1621 ggtaacccaa attattgtca cccatgattt agtatttgca gaaaatgtcg gtgatcaatt
     1681 actaaaagtt tccccaattc attaagaaag gagaagatag agatgaaaaa aaccagtttt
     1741 ctattcgctt tgatcgcagg tcttcttctt tttgcaggct gttcgaacaa aaaaacttca
     1801 gcagataatt gggcgactat caacgaagag aaaaaaatcg ttataggttt agacgataca
     1861 tttgttccta tgggatttag agataaagac gggaagctca caggatttga tattgattta
     1921 gccaaagcag tctttgaaca atatgggatt acggtagact ttcagcccat tgactggtca
     1981 atgaaggaat ttgaactgaa caatggaacg atcgatttga tatggaacgg ctattccaaa
     2041 acgccggcaa gagaaaagaa agtgcaattt acaaaacctt atatggaaaa cgatcaagtg
     2101 ctgatcacac ctaaagaatc aggaatcaca aactttggac aaatgaataa taagagatta
     2161 ggtgcgcaaa atgggtcatc tggttatgat gtatttacga aacaaccaga agtattaaaa
     2221 gagatagtca aaaaccaaga tgctgttttg tatgatagtt ttaatgaagc attgatcgat
     2281 ctgaaaagca gacgggtcga tggtctgctg atggataaag tatacgcaga ctattatttg
     2341 aaacaaagaa acgaactaga agacttcaac ataacaaaag gttcttatga gagtgaggat
     2401 tttgcagttg gtgcacgcaa gtcagatagt acattagtga aaaaaatcaa tgaagcccta
     2461 gatcaactag agaaaacagg tgaattccaa aaaatatccg ataaatggtt tggcgaagat
     2521 gttacgccga gaaattaaca aataggggac tgtgacaaaa gacgtgtcac agtccctttt
     2581 tccgaataaa cagtgttctc ctgccaactc cttgtgactc ggagctgaat ggcaggctca
     2641 caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg tattaagcca aatttatgca
     2701 aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa gcgacttttt
     2761 cacaacctcc tttacataat tttattttgt tgtggaaggg gtttcgtgtt attctaaaag
     2821 aggaacgatt gaaggttata ggaaaggaag agatgaatgg aacacaaaga acttacctca
     2881 tttccaaaag attttctgtg gggctctgct tctgcagcgt atcaagtaga aggagcttgg
     2941 caagaagatg ggaaaggaga atcagtctgg gatcgatttg tacggatacc tggaaagaca
     3001 tttaaaggaa cgaacggtga tttggcagta gaccactatc atcgttataa agaagatatt
     3061 gctttaatga aagaacaagg tctgaaagct taccgcttct ctgtagcttg gacacggatt
     3121 tttcctaatg gacgaggaga agtcaatcaa gctgggctcg ctttttatga gagattgatc
     3181 gacgaattga ttgaaaacga tattgaacct atgttgactt tatatcattg ggatcttcca
     3241 caagcattgc aagatgaata taatggttgg gaatcacgtc agatcattga agatttcact
     3301 cattatgcag aaacattatt tgaggcattc cgcgggaaag tccattactg gattagtttg
     3361 aatgaacaaa atatcttcac ttcattaggt tatttgttag cagcacatcc gccaggtgtg
     3421 acagacccga aacgaatgta tgaagttaac catattgcta atttagcaaa tgcatctgtc
     3481 atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg
     3541 ccaaattatc caattgacag taatcctgaa aatgttctgg ctgctgaaaa tgcagaagac
     3601 ctgatggcca attattggat ggatgtctat atgtggggaa aatatccgat tgctgccatg
     3661 aggttcttag aagaaaaagg atgggcacca acgattgaag ctggcgatgc agaattgcta
     3721 gaatctgcta aaccagattt tcttgggatc aattactatc aaacggcgac gaatgcattc
     3781 aatccattaa acggagttgg tgctggaaaa atgaatacga caggaaaaaa aggttcatca
     3841 gaagaaactg gagttcctgg aatgtataaa aaagttgaga atccatttgt cgaacgaaca
     3901 aattgggact gggagattga cccagaagga ttaaggatcg gactgcgtcg tatcacaagc
     3961 cgttaccgca tgccggtact gattacggaa aatggcttag gagagtatga caagttgaca
     4021 gaagataaac aaatccatga cgactatcga atcaattact tacaaagcca cgtcaaagcc
     4081 atcaaagaag caatttcgga tggagcagaa gtcctaggct attgcacctg gtcttataca
     4141 gatttgctta gctggctgaa cggttatcaa aagcgatatg gatttgtata tgttgatcaa
     4201 gacgaaacac aagaaggatc tttggaacga tataagaaag acagttatta ctggtaccaa
     4261 aaactgattg aagaaaatgg agagaacctt tagttcacat acttaaaaca agaaggtacg
     4321 gctggatatg gccgtgcctt ttcttgataa aatcatttag aaattaagaa aaactgtaaa
     4381 ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac aaaactttag ttcccttact
     4441 tttttttgta taatgaaact tgatgaaatg agggataata aatggcacaa aagaaacggc
     4501 ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca aaaacagcag caggaacaaa
     4561 tgctgacgat tggtgttggg tttgccttga ttttatttgc tgtcttcggc tttttaaaat
     4621 taggtttttt aggaatattg attgcaaacg gctttcggat cattgcagga aatacttacc
     4681 aaatcctctg tcttttatta gccgtattag gattttggat tgtcattaag aatacagagt
     4741 tttcaattgg taaaaaccga cgctggtttg gcggcatact cttttatttc ggtatcttat
     4801 tattgttaca tgcccattta tttggcaaac tccatactgg ggaaccaaat atcatgggga
     4861 cgacatggga tttgttagcc agtgatataa aacaaagtca agtagacaat aacgttggtg
     4921 gtgggatgat tggagcaatc ctttatcatt ttacttactt cttgattgca cagcctggaa
     4981 gttatttagc agcagtgcta ttactggcag gaggagcatt cctgttcagt aatctggaag
     5041 gctatcagct gttaaatggt atccaatcaa ttggagaacg agtacaggaa ttattggaag
     5101 gtgatcctgc caagcaagct cgaaagcaag cggcaaagga agagagaatg aaacagcgcg
     5161 cagaagcgaa agaagcaagg cgcttagctg caaaagaagc tgcagaaaaa gaagctgcag
     5221 aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa tactgctgat gaatggcagg
     5281 aaccagaaaa tacagagccg gagcagctaa gttttgttcc aatcgacagt tttcaagaaa
     5341 atatccatcc tgcgaatcta gagaaaccag taccagatac accaaaacag accaatacag
     5401 cagaaggttt ttcagatgag ttgccagagg atgacggtac ctcattagaa tttgagattg
     5461 aagcagaaca ggaaaatcaa gactatgaat taccgacagt tgatttgctt gattctatac
     5521 caactgtgga tcaaagtgat gagtataaaa aaattgaaaa aaatatcggc gtattagaac
     5581 aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa agccagctta ggaccatccg
     5641 tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt aagtaaaatt gtgaacttaa
     5701 cagatgatat cgcgcttgct ttagcggcga aagatgtccg gatggaagca ccgattccag
     5761 gtaaatcact gattggtatt gaagtaccaa atggaaagat cagtatggtt tcctttagag
     5821 aaatcatcga agcacaacca aaccacccag ataaattact agaagtacct ttaggacgtg
     5881 atgtttctgg acgggtacaa acggctgatc tttcgaagat gcctcactta ctcgtggctg
     5941 gttcgactgg tagtgggaag tcagtagcaa ttaatgggat tatcacaagt atattaatgc
     6001 gtgctaagcc ccatgaagta aaactgatga tgatcgatcc gaaaatggtt gaattgaata
     6061 tgtacaacgg catacctcat ctgttgacgc cggtcgtgac gaaccctaga aaagcagcgc
     6121 aagccttaca aaaagtagta caagaaatgg aagaacgata tgaaaaattc gcggcaaccg
     6181 gagtaagaaa tatttctggt tacaatgaat ttgttcaaca gaaaaacttg gaaaacggaa
     6241 cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga tgaattagct gatttgatga
     6301 tggtagccag caatgaagtt gaagatgcga ttattcgttt agcgcaaatg gctcgggcag
     6361 cggggattca tatgatccta gctactcaac gtccaagtgt ggatgttatc acaggaatca
     6421 tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc aagcggtaca gattctcgga
     6481 cgatcattga ttctaatggg gcagaaaagc tcttagggcg aggagatatg ctattcttgc
     6541 caatgggtga gaataaaccg atccgtgtgc aaggagcatt catttctgac cacgaagtag
     6601 aacgggtcgt tcaatttgtt acagatcagc aagaagctca ttatgaagag aaaatgatgc
     6661 cgacagatga agttgaaacg acaggtgctc cagaacagcc acaagatgag ctgtttgaag
     6721 aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc caaagaagat
     6781 ttcggatcgg ttacaaccga gcagcacgtt tagtagatga actggaagct catggagttg
     6841 ttggcccgtc agaaggaagc aagcctcgga aagtattcat tgagcaggaa gaaacatcag
     6901 aaacgttgcc ggacgataat gacatgtaaa acttgtgtga tgaagggttg aaaaacgaat
     6961 aaccaataaa aagacaaggc atgatgatac caaaagaaaa aatggttttt ttccatcttt
     7021 tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt ctattcgatt
     7081 tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg aaactttgag
     7141 aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa tgaagaatta
     7201 atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt ttgggaatgg
     7261 ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa acaattgatc
     7321 gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt ccaagaagct
     7381 tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat tgagactgta
     7441 ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg atgtaatatc
     7501 ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac tgctggagaa
     7561 attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct tggcgaacgc
     7621 gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga caatgtaatg
     7681 gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg gcacattaca
     7741 gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg cttgcaagtc
     7801 aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat catgcggatc
     7861 aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt agagaaaaca
     7921 aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag accagagcat
     7981 gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt caacttgatt
     8041 ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc tgatgtattg
     8101 agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt
     8161 acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc
     8221 aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt tcttaattaa
     8281 ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa agcttttttt
     8341 gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat
     8401 gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt cttgtgtagc
     8461 atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga tttcataagg
     8521 cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta aatcggtcgt
     8581 gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca taaacacata
     8641 gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta caccataact
     8701 gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac cgtaatagaa
     8761 tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca atccggtagt
     8821 aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt cggcactatc
     8881 tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc cgatttggat
     8941 tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag attcgacttc
     9001 tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt cggcaataat
     9061 tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc cgtccattac
     9121 gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg aaccaatagt
     9181 gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag tatcgcaagc
     9241 acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat gctggatcaa
     9301 ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga caacctctat
     9361 ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag tcttccctcc
     9421 aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt catacgcatc
     9481 tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt ccttctttct
     9541 tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg tagatggagt
     9601 agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc ttgaaatcat
     9661 caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat aatgaatttt
     9721 tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct aaaaattcta
     9781 aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt ggtttttttt
     9841 aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc aattgatggc
     9901 tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta ttttataagt
     9961 aagataaata atgaaagaat agagatactg attggtagct aaagattttt gagcgagaac
    10021 atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag acgtatgata
    10081 atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga tttactgaga
    10141 ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga aatgatgaat
    10201 ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt tattggtgca
    10261 atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga aagctcccga
    10321 cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt agcttccaga
    10381 ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac gttaagtcaa
    10441 acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct tcgtgaacat
    10501 ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc aaatgcaaat
    10561 ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc aaattctcaa
    10621 taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg aatttacgaa
    10681 aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta cttttggaac
    10741 accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg
    10801 aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    10861 caccgtttat tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat
    10921 tcttttttat ctttgtatgg caataataga caatataacg aaaattattt aatggctttt
    10981 ggaagaccat ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca
    11041 cgaaaaaatc attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt
    11101 cattaaactc ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg
    11161 tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg
    11221 ctggatgtta tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag
    11281 atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag
    11341 ttgcaacaga tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc
    11401 ttgatgaaga cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag
    11461 acggattaac ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg
    11521 ttacagcagc ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg
    11581 aatatgctta cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag
    11641 ataaatcaga actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga
    11701 aacaaacacc atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat
    11761 ccgtagtgga agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg
    11821 gaccattcac attagaagat tttgatggac caggaacaga tacagaatgg acttataaga
    11881 aaaatgatca ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag
    11941 taaaagaatc ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct
    12001 tgtctggtga gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag
    12061 cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga
    12121 acctaagaaa agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag
    12181 gtgacggatc agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa
    12241 cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa
    12301 aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga
    12361 tggcttctga tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag
    12421 aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc
    12481 gctcaacttc gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc
    12541 caagcagctt tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca
    12601 atgcagaata tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag
    12661 cccgttggga tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc
    12721 cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc
    12781 atgcggcagg cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac
    12841 cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact
    12901 tatcctaaca aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg
    12961 atcagccaaa agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt
    13021 ttctttcggt tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc
    13081 gaagaaaaga cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga
    13141 tcattagata attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc
    13201 agaatcagta gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt
    13261 tatttttggt tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg
    13321 ggaaacatta gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat
    13381 cacgctttgg ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc
    13441 atatacgaat caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg
    13501 tttggataaa ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga
    13561 ttttggaatt tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat
    13621 cggaccatcc ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat
    13681 tttaggaacg atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt
    13741 agcgatatta ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt
    13801 tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt
    13861 accaacaatt gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga
    13921 aatggtggaa gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg
    13981 ttgggaaatc gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt
    14041 aggaccgtta gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat
    14101 tcctggtatc ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat
    14161 ggctgtaacg atcctttact catttatgct gatttttgtt attttgatcg tggatctatt
    14221 gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat
    14281 gataccaaag cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca
    14341 aaaaaatacc gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga
    14401 ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt
    14461 catcattttg atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa
    14521 cgtggcttat atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg
    14581 aaaagcaatg gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa
    14641 cgtaaatttc tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat
    14701 gggaatgaga atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg
    14761 tgtagcttac gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg
    14821 ttttttggaa atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt
    14881 ttttgaacca ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat
    14941 ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg
    15001 attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc
    15061 tagtgtcatt attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt
    15121 tttaagcttt atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag
    15181 cgaagggtac aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt
    15241 atcggttatc atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc
    15301 taaaatgaaa gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct
    15361 tttgatacgt atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa
    15421 ggagaaacat tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc
    15481 atcatgcgct tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt
    15541 aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg
    15601 atgattttcc aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc
    15661 gctgagtccc ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta
    15721 gaattgctta aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat
    15781 cagttctcag gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg
    15841 gaaatactga ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt
    15901 ttggagctgc tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat
    15961 gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc
    16021 gttgaagttg gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga
    16081 ctgcttggtt caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt
    16141 tctccaccgg atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc
    16201 gcgatgaaaa tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag
    16261 gcagcaactt ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa
    16321 cgtcgctggg ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct
    16381 gatgccaaat aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa
    16441 gaatgaagta catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg
    16501 tttagttgga gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa
    16561 tgaacctact gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa
    16621 aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc
    16681 gttgaatcct cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact
    16741 agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt
    16801 gaatccaagc cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat
    16861 tggtattgca cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc
    16921 tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga
    16981 acatcaattg acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga
    17041 ccgaatcggt gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta
    17101 taatcacggt gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc
    17161 agaccatgag agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt
    17221 acgtcaactt cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc
    17281 atattacgca aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg
    17341 acatagatgc taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa
    17401 ttgattcgta cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag
    17461 aaaagttggc aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa
    17521 gaatacccat tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg
    17581 gatcgggctt ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa
    17641 aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat
    17701 ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt
    17761 gaaggatcga ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca
    17821 ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc
    17881 tatcatccga agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt
    17941 caccagacag aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc
    18001 gctcaccata gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa
    18061 gtaatggatc tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct
    18121 gggaaattgg tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca
    18181 catgctgatt tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc
    18241 caatggtata ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc
    18301 gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa
    18361 atgaatgaaa ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt
    18421 ttgtttctag gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac
    18481 aatagcagaa aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt
    18541 ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt
    18601 tttttcttga atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc
    18661 ttgagctgat agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg
    18721 aaatattgga gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct
    18781 aagaatttag caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt
    18841 agaacttgcg gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt
    18901 tgactatggt caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc
    18961 gagatagcca cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac
    19021 cagcgtactt ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag
    19081 tagctgactg tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt
    19141 tgctcaatat tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg
    19201 ctttctccag ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga
    19261 taaggatgcc cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag
    19321 cacatcagta cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt
    19381 tgataccagt taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct
    19441 tctttagaag caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc
    19501 ttattgtagc agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt
    19561 taaatatgaa aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc
    19621 aatactttat accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca
    19681 atgataaaac gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta
    19741 aaataaccat acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa
    19801 gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa
    19861 gatagatagt ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag
    19921 atatgaaaca aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat
    19981 tgaacgtaga tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat
    20041 gcttttttta tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga
    20101 gccccgtggc agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca
    20161 aacttcatat cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac
    20221 ggttgatggg ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg
    20281 aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg
    20341 atgtcaagaa attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag
    20401 atgattacca taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata
    20461 tttacttagg tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa
    20521 cacaacttgc agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa
    20581 gtggacacga agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg
    20641 cagaccgtct attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta
    20701 aaaacgaaat gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa
    20761 caacttttac ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga
    20821 tcgatgcatt atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat
    20881 tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata
    20941 cgatctattg gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg
    21001 ggcatggctg gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt
    21061 atcctgaaat gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg
    21121 ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt
    21181 atgatctggc aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat
    21241 attgtgatgg actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt
    21301 ctacgacggc tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca
    21361 atacagcatt ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa
    21421 cagaaccatg ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga
    21481 tccatttagc tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa
    21541 cgccagagaa gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat
    21601 tacactttgg cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc
    21661 cacgtttaga tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc
    21721 aaacaaacga agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag
    21781 aaaaacaaat caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca
    21841 actgtcaaaa agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca
    21901 gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga
    21961 tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca
    22021 gtcaaggaat gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac
    22081 caaaatccat gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag
    22141 ttgtgtcata cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    22201 acttgaagcc aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa
    22261 gaagtacgag cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa
    22321 cagaaaaaaa gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct
    22381 tttttattgt ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta
    22441 tatctaacag tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt
    22501 tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc
    22561 gatgattgtt ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat
    22621 ttttgtcttc aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac
    22681 ataccagaaa atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg
    22741 taacaagatc aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat
    22801 gacttcacta agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt
    22861 catccgccag ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa
    22921 aataccgata ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat
    22981 cacaggacct ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg
    23041 tgataacgtt gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga
    23101 ctgctgttgt tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg
    23161 ataagaacgc ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata
    23221 cgaaaaataa aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa
    23281 aaaagaatgg taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga
    23341 agaaagagga tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta
    23401 gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact
    23461 gctagtactc agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg
    23521 atctgtgctg gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct
    23581 gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa
    23641 agcttgattt actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg
    23701 acgaacttat tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt
    23761 tctgtgacag ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg
    23821 ttgattgtta aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga
    23881 caaggaactg tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga
    23941 ttgagcagtg cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca
    24001 atggcagggg aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg
    24061 agttttgtaa cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt
    24121 gatcatcttg ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga
    24181 atcggtggaa agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt
    24241 tatacactaa ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc
    24301 agtaaacagc tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc
    24361 cagttagcag tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg
    24421 ttgatttttg tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga
    24481 aaaaaagcgc cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt
    24541 attgcgattc tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta
    24601 gcattgatcg gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa
    24661 aaacgctcag aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc
    24721 tagatcttcg tgctttttct atttcaataa aacaagttct tctattttga tttttgctac
    24781 ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt
    24841 tgattctcat acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca
    24901 gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat
    24961 tgaaaagtct ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca
    25021 tccgacagag gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac
    25081 tttaccaaag gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc
    25141 gaaagaagtc caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct
    25201 accaatcagt attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga
    25261 agatgttcga gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa
    25321 acgatttctt gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc
    25381 attagacgtc caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga
    25441 tgccccttat ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg
    25501 ttatacagta gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca
    25561 aacatggaca aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc
    25621 aagagattat agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg
    25681 cagatacgat agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac
    25741 atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa
    25801 taagaaaaat cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac
    25861 aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt
    25921 tagaagcatt gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca
    25981 ctagacaaga ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag
    26041 aaaaactagg tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt
    26101 tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac
    26161 aacatgagtg aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa
    26221 catggttttt catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta
    26281 aggaaaatcg tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca
    26341 ggaatcggtg cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag
    26401 atcgatgatc gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact
    26461 attgtacatc aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat
    26521 gaggagcttc cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg
    26581 atgcattttc ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa
    26641 gtagctgatc gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct
    26701 gttcaatatt atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct
    26761 cagccaaatg tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa
    26821 gtgacagacg aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa
    26881 acgctttgga ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta
    26941 gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta
    27001 caagaattcg ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg
    27061 tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    27121 gttcctcatg tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg
    27181 tttaagaaag aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa
    27241 aacagcttct catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac
    27301 tattatgccg aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt
    27361 gatcaaaaaa ttcttatact tgatactcac actcattttg tatggattta gaaaagaata
    27421 tgtgtcttga cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca
    27481 tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta
    27541 ttgattggga tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct
    27601 tcctttactt tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt
    27661 gcagtggctg ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc
    27721 gcattgcgag cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag
    27781 catccgagta tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg
    27841 ttcaattttg tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt
    27901 tactttattg gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc
    27961 atcttgatgg ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt
    28021 atagcaggaa cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa
    28081 aatcggaaag agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc
    28141 aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc
    28201 ctaaaaagag ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag
    28261 tttgatgact tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag
    28321 gtcgactatt tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt
    28381 agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc
    28441 tgatttcact acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc
    28501 tggacgaata ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt
    28561 ttttccttca ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa
    28621 tgtcacatta aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac
    28681 gggtgtatca taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat
    28741 aacgattcgg tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt
    28801 tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc
    28861 atctaaattt gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg
    28921 tcccaatgcc acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta
    28981 ttctgtcaga cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc
    29041 atatttacgt aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa
    29101 tgcgtagttt tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac
    29161 ttttccgccg atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa
    29221 agtagttgac tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat
    29281 tgatagatta aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt
    29341 tttcagag
//
LOCUS       DANKAY010000026        28866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000026 DANKAY010000000
VERSION     DANKAY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            7..624
                     /locus_tag="PWY98_001125"
     CDS             7..624
                     /locus_tag="PWY98_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036888.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="PWY98_001126"
     CDS             1644..2813
                     /locus_tag="PWY98_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2036889.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="PWY98_001127"
     CDS             3286..3840
                     /locus_tag="PWY98_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2036890.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="PWY98_001128"
     CDS             complement(4013..4486)
                     /locus_tag="PWY98_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2036891.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="PWY98_001129"
     CDS             4498..5034
                     /locus_tag="PWY98_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2036892.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="PWY98_001130"
     CDS             5074..5445
                     /locus_tag="PWY98_001130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2036893.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="PWY98_001131"
     CDS             5729..7000
                     /locus_tag="PWY98_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2036894.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="PWY98_001132"
     CDS             7245..8270
                     /locus_tag="PWY98_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2036895.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="PWY98_001133"
     CDS             8564..9673
                     /locus_tag="PWY98_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2036896.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="PWY98_001134"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="PWY98_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="PWY98_001135"
     CDS             10668..11492
                     /locus_tag="PWY98_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2036897.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="PWY98_001136"
     CDS             11524..12834
                     /locus_tag="PWY98_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL2036898.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="PWY98_001137"
     CDS             12982..14241
                     /locus_tag="PWY98_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2036899.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="PWY98_001138"
     CDS             14251..15123
                     /locus_tag="PWY98_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2036900.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="PWY98_001139"
     CDS             15135..15965
                     /locus_tag="PWY98_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2036901.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="PWY98_001140"
     CDS             16005..18188
                     /locus_tag="PWY98_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2036902.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="PWY98_001141"
     CDS             18249..19469
                     /locus_tag="PWY98_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL2036903.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="PWY98_001142"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="PWY98_001142"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL2036904.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="PWY98_001143"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="PWY98_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="PWY98_001144"
     CDS             22431..22640
                     /locus_tag="PWY98_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2036905.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="PWY98_001145"
     CDS             22633..22782
                     /locus_tag="PWY98_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL2036906.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="PWY98_001146"
     CDS             22936..23601
                     /locus_tag="PWY98_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2036907.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="PWY98_001147"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="PWY98_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2036908.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="PWY98_001148"
     CDS             25304..25537
                     /locus_tag="PWY98_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2036909.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="PWY98_001149"
     CDS             25534..25941
                     /locus_tag="PWY98_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2036910.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="PWY98_001150"
     CDS             complement(27078..27596)
                     /locus_tag="PWY98_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2036911.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            28714..>28866
                     /locus_tag="PWY98_001151"
     CDS             28714..>28866
                     /locus_tag="PWY98_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2036912.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         9935 a         4350 c         5290 g         9291 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagcttg gataggctac aattaactag acagaaaaat taaggtgtgt
    28681 agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt
    28741 tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa
    28801 atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa gcaatctaaa
    28861 acgagt
//
LOCUS       DANKAY010000027        28587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000027 DANKAY010000000
VERSION     DANKAY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..1835)
                     /locus_tag="PWY98_001152"
     CDS             complement(<1..1835)
                     /locus_tag="PWY98_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2036913.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="PWY98_001153"
     CDS             complement(1832..2536)
                     /locus_tag="PWY98_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2036914.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="PWY98_001154"
     CDS             complement(2548..4857)
                     /locus_tag="PWY98_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2036915.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="PWY98_001155"
     CDS             complement(5052..5516)
                     /locus_tag="PWY98_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036916.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="PWY98_001156"
     CDS             complement(5516..6142)
                     /locus_tag="PWY98_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2036917.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="PWY98_001157"
     CDS             complement(6149..6514)
                     /locus_tag="PWY98_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2036918.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="PWY98_001158"
     CDS             complement(6504..6842)
                     /locus_tag="PWY98_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036919.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="PWY98_001159"
     CDS             complement(6832..7158)
                     /locus_tag="PWY98_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2036920.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="PWY98_001160"
     CDS             complement(7136..7417)
                     /locus_tag="PWY98_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2036921.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="PWY98_001161"
     CDS             complement(7419..8783)
                     /locus_tag="PWY98_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2036922.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="PWY98_001162"
     CDS             complement(8796..9365)
                     /locus_tag="PWY98_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL2036923.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="PWY98_001163"
     CDS             complement(9337..10524)
                     /locus_tag="PWY98_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2036924.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="PWY98_001164"
     CDS             complement(10588..12315)
                     /locus_tag="PWY98_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2036925.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="PWY98_001165"
     CDS             complement(12312..12764)
                     /locus_tag="PWY98_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2036926.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="PWY98_001166"
     CDS             complement(12876..13256)
                     /locus_tag="PWY98_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2036927.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="PWY98_001167"
     CDS             complement(13253..13639)
                     /locus_tag="PWY98_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036928.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="PWY98_001168"
     CDS             complement(13641..13919)
                     /locus_tag="PWY98_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036929.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="PWY98_001169"
     CDS             13967..14695
                     /locus_tag="PWY98_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL2036930.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="PWY98_001170"
     CDS             complement(14841..15317)
                     /locus_tag="PWY98_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2036931.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="PWY98_001171"
     CDS             complement(15277..15624)
                     /locus_tag="PWY98_001171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036932.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="PWY98_001172"
     CDS             complement(15611..15796)
                     /locus_tag="PWY98_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036933.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="PWY98_001173"
     CDS             complement(15797..16069)
                     /locus_tag="PWY98_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL2036934.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="PWY98_001174"
     CDS             complement(16066..16269)
                     /locus_tag="PWY98_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036935.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="PWY98_001175"
     CDS             16394..16606
                     /locus_tag="PWY98_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036936.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="PWY98_001176"
     CDS             complement(16639..16833)
                     /locus_tag="PWY98_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036937.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="PWY98_001177"
     CDS             complement(16846..17028)
                     /locus_tag="PWY98_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036938.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="PWY98_001178"
     CDS             complement(17025..17165)
                     /locus_tag="PWY98_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036939.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="PWY98_001179"
     CDS             complement(17655..18020)
                     /locus_tag="PWY98_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036940.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="PWY98_001180"
     CDS             complement(18060..18362)
                     /locus_tag="PWY98_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2036941.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="PWY98_001181"
     CDS             complement(18362..18661)
                     /locus_tag="PWY98_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036942.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="PWY98_001182"
     CDS             complement(18654..18818)
                     /locus_tag="PWY98_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036943.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="PWY98_001183"
     CDS             complement(18824..19093)
                     /locus_tag="PWY98_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036944.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="PWY98_001184"
     CDS             complement(19105..19854)
                     /locus_tag="PWY98_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036945.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="PWY98_001185"
     CDS             complement(19851..20543)
                     /locus_tag="PWY98_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036946.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="PWY98_001186"
     CDS             complement(20549..21220)
                     /locus_tag="PWY98_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2036947.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="PWY98_001187"
     CDS             complement(21213..21554)
                     /locus_tag="PWY98_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036948.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="PWY98_001188"
     CDS             complement(21732..22430)
                     /locus_tag="PWY98_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2036949.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="PWY98_001189"
     CDS             22517..22987
                     /locus_tag="PWY98_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036950.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="PWY98_001190"
     CDS             complement(23030..23209)
                     /locus_tag="PWY98_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036951.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="PWY98_001191"
     CDS             complement(23196..23534)
                     /locus_tag="PWY98_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2036952.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23550..23753)
                     /locus_tag="PWY98_001192"
     CDS             complement(23550..23753)
                     /locus_tag="PWY98_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2036953.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23768..24544)
                     /locus_tag="PWY98_001193"
     CDS             complement(23768..24544)
                     /locus_tag="PWY98_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2036954.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24843..25163
                     /locus_tag="PWY98_001194"
     CDS             24843..25163
                     /locus_tag="PWY98_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2036955.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25181..25612
                     /locus_tag="PWY98_001195"
     CDS             25181..25612
                     /locus_tag="PWY98_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2036956.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25742..26203
                     /locus_tag="PWY98_001196"
     CDS             25742..26203
                     /locus_tag="PWY98_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2036957.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            26237..26872
                     /locus_tag="PWY98_001197"
     CDS             26237..26872
                     /locus_tag="PWY98_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2036958.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            26985..27620
                     /locus_tag="PWY98_001198"
     CDS             26985..27620
                     /locus_tag="PWY98_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036959.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            27919..>28587
                     /locus_tag="PWY98_001199"
     CDS             27919..>28587
                     /locus_tag="PWY98_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HDL2036960.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQI"
BASE COUNT         8368 a         5731 c         4479 g        10009 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct
    23581 ataattctgt ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt
    23641 ttgctaattc caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca
    23701 tattctttga ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa
    23761 ctcctttcta accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta
    23821 attcgtagat aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc
    23881 caccatcggc tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt
    23941 ttttgcacaa taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt
    24001 tattcatctg ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat
    24061 ctgtagaaga taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt
    24121 gttctgcgat taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg
    24181 tatctggatt aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc
    24241 taattgctgg caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt
    24301 ttgattttaa gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt
    24361 gacctgaggt cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag
    24421 catctttagt gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt
    24481 catcatttac taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt
    24541 tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat
    24601 tcaattgaca gtatctgagt aatactctat actaagagca taattaaata agacataaaa
    24661 acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg ttttttgttg
    24721 tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc agaattagtc
    24781 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
    24841 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
    24901 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
    24961 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
    25021 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
    25081 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
    25141 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
    25201 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
    25261 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
    25321 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
    25381 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
    25441 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
    25501 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
    25561 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
    25621 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
    25681 ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa
    25741 aatggaaaaa gagattttaa taaaatctcc aggaaaaacc ttgattaaag taactgatga
    25801 ttctatttca attattagaa aaggatttat aaatttagtt aatcaaggta ttaaaggaga
    25861 aaaaactatc ccctttaaaa atatatcagc cgtacaatta aaaaaaccag gtatgagtaa
    25921 tggttatata caatttacct tgctaggcgg aaacgaaagt cgcggaggta tattagcggc
    25981 cacgaaagat gaaaacaccg ttatgtttac aaaaaaatat tggaatgaaa tggaaaattt
    26041 aaaaaaatat atagagaagc aacaatctat attagataat aattccaaag aaacacaaat
    26101 ttcaagtgca gatgaaatta aaaagtataa agagttgtta gatgaaggga ttattaatca
    26161 agaagaattc gattttaaga aaaaagaatt gcttggttta taatattcaa tatcaaaaga
    26221 gaaagaggat ctattaatgg acggtatatt tggttttact ggtattgttt tatttattat
    26281 aggtttaatc atgttaattg taagatttat caaaaaaaca aataagaaaa cacctatgat
    26341 tctccttatt atcgggatta tttttactac cgtaggtttc tcgctttcct catcaaacga
    26401 ggcaaatgac aataacaaac aggaaagctc atcatctgat actcaaacta ctacatctaa
    26461 cagcagcaat aaaagcctaa ctcaatttga agaattttta gagacaaata atcatgactg
    26521 gggtgctttc ttagatagtt attattctat tactccagct actgagcaaa atacagcttt
    26581 tactaaatat attagtggta aaacatatac ttttgaagga actgttatcg aatcaatgac
    26641 tactagaata gccattatag cagacaaaga gtacgatgat aaaagttgga atgatatttc
    26701 tgcaacacct aaagtttcat atgtaatttt tgcgaaggac ttaaacaatg ctgatacctt
    26761 tacaaagggc gataaagtaa cttttacagg agaaattagt tcaagaggat cgaacataga
    26821 aaaatcctat gctcaatggg atatgattaa tagtaaagta agtaaaaaat aaatgaacca
    26881 cgccccacta agagttagat tgtgcacaat aataaaacct atacaaaaga gtgccatcag
    26941 gagtcacaat gcttgtaaag gaaatgacaa aataggaggt aactatggat aatagcaatg
    27001 aaaaactaac caaaaaagaa tatcttgaat taatagttga aggcggtatt ggagcaattc
    27061 cttatattgg tggcgttgtg cagtcacttt attttgggtc taaaaacgag aagcgtttca
    27121 aacgcatcga gaaattctac aaaaatttaa acgaatcatt agaaaacatt cgcgatcaaa
    27181 ttccagaaga tgttttcaac ttagagaaca aggaccaact cattggaata ttcgaaggga
    27241 taaatgacga ggtagaaaaa tcaaaggcac aaaataaaat agatatgtat aaaaatcttt
    27301 ataagaattg tttacttcga attaacaacg cttcatggga taacgaggag tattatcttc
    27361 ttgtactgaa tcaattaaca tcgattgaga ttcaattgtt agcatgctta atgagtcgtg
    27421 gaaacgaaaa tttcactgga aatatcagca tgtctggcta ttcacaagag ctaatagatg
    27481 gtagcttaaa tagattatct gattttggtc tgttagaaaa aacgattagc tccatagtat
    27541 taggtgatgt cggcaaacaa aatatgggct acaaaatatc tcatttagga attcaattta
    27601 ttaactcaac tcttacttaa gttttctttc taatatgaca tctagaccta tcagcaatgc
    27661 aacaataaaa aaccagacat accaataatc aacaaataat ctaaactctg gaagttcagt
    27721 tattctttta aaaatcactt taatcataat aaacacctct gattggatga tctaatacac
    27781 atccaattat accattaaaa cgacaaatag cacatccttg cgccggcaag caaaaaggat
    27841 gtgcaaaaaa taataaaacc caaacaatgg gcttctttat agttcctatt gtatcagaga
    27901 aagagagctg attcaattat ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa
    27961 atttcataca aaaaagatga cggaaaatac acgaagataa gaaaatcagg atttaagaca
    28021 aaaggagaag caaaagatgc cgccaacgaa ttagaatata acttgaacaa aggccttaaa
    28081 ggggatcgca aaaatctatt attatcagat tactttgagg attggatgca actttataaa
    28141 gaaggaacag tatctcctat cacttataga aaatacgaag atacgttaat gaacataaag
    28201 aaatatatgc cagcggtatt gatttctgat ttagatagag tgggatatca acgcttttta
    28261 aataagtatg ctaaagacca tgtaaaatct accgttatta agtttaataa ccatattaga
    28321 gcgtcgttga aagatgccgt agaagaagga ttaattccgt ttgatccaac tagaaaagca
    28381 gtaatcaaag gaaaagattc attgaagcca aaagaagata aatatttaga ttatgatcaa
    28441 tttaaatctt taatgaaact cgtagaagaa aacctttctg cgcagtactc ttctcctatg
    28501 ctcgtgttag ttgctggtgc cactggaatg cgatttgctg aacttctagg attaacatgg
    28561 gaagatatcg atttcgaaga tcaaatc
//
LOCUS       DANKAY010000028        26979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000028 DANKAY010000000
VERSION     DANKAY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(144..1112)
                     /locus_tag="PWY98_001200"
     CDS             complement(144..1112)
                     /locus_tag="PWY98_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2036961.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1344..2177
                     /locus_tag="PWY98_001201"
     CDS             1344..2177
                     /locus_tag="PWY98_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2036962.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2211..3050
                     /locus_tag="PWY98_001202"
     CDS             2211..3050
                     /locus_tag="PWY98_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2036963.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3076..3636
                     /locus_tag="PWY98_001203"
     CDS             3076..3636
                     /locus_tag="PWY98_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2036964.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3835..5556
                     /locus_tag="PWY98_001204"
     CDS             3835..5556
                     /locus_tag="PWY98_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2036965.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5721..6863
                     /locus_tag="PWY98_001205"
     CDS             5721..6863
                     /locus_tag="PWY98_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2036966.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6879..8090
                     /gene="thiI"
                     /locus_tag="PWY98_001206"
     CDS             6879..8090
                     /gene="thiI"
                     /locus_tag="PWY98_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2036967.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8663..9310
                     /locus_tag="PWY98_001207"
     CDS             8663..9310
                     /locus_tag="PWY98_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2036968.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9439..9655
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9703..12348
                     /locus_tag="PWY98_001208"
     CDS             9703..12348
                     /locus_tag="PWY98_001208"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2036969.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12658..13971
                     /locus_tag="PWY98_001209"
     CDS             12658..13971
                     /locus_tag="PWY98_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2036970.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13961..14614
                     /locus_tag="PWY98_001210"
     CDS             13961..14614
                     /locus_tag="PWY98_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2036971.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14669..15361
                     /gene="radC"
                     /locus_tag="PWY98_001211"
     CDS             14669..15361
                     /gene="radC"
                     /locus_tag="PWY98_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2036972.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            15511..15711
                     /locus_tag="PWY98_001212"
     CDS             15511..15711
                     /locus_tag="PWY98_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2036973.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(15965..17644)
                     /locus_tag="PWY98_001213"
     CDS             complement(15965..17644)
                     /locus_tag="PWY98_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2036974.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(17646..17858)
                     /locus_tag="PWY98_001214"
     CDS             complement(17646..17858)
                     /locus_tag="PWY98_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2036975.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            18251..19981
                     /gene="efrA"
                     /locus_tag="PWY98_001215"
     CDS             18251..19981
                     /gene="efrA"
                     /locus_tag="PWY98_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2036976.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            19978..21738
                     /gene="efrB"
                     /locus_tag="PWY98_001216"
     CDS             19978..21738
                     /gene="efrB"
                     /locus_tag="PWY98_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2036977.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            21833..22030
                     /locus_tag="PWY98_001217"
     CDS             21833..22030
                     /locus_tag="PWY98_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036978.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            22183..22668
                     /locus_tag="PWY98_001218"
     CDS             22183..22668
                     /locus_tag="PWY98_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2036979.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(22772..23365)
                     /gene="clpP"
                     /locus_tag="PWY98_001219"
     CDS             complement(22772..23365)
                     /gene="clpP"
                     /locus_tag="PWY98_001219"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2036980.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            23545..24072
                     /locus_tag="PWY98_001220"
     CDS             23545..24072
                     /locus_tag="PWY98_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036981.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            24163..24900
                     /locus_tag="PWY98_001221"
     CDS             24163..24900
                     /locus_tag="PWY98_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2036982.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            24904..25821
                     /gene="miaA"
                     /locus_tag="PWY98_001222"
     CDS             24904..25821
                     /gene="miaA"
                     /locus_tag="PWY98_001222"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2036983.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            25823..>26979
                     /gene="hflX"
                     /locus_tag="PWY98_001223"
     CDS             25823..>26979
                     /gene="hflX"
                     /locus_tag="PWY98_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2036984.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAY"
BASE COUNT         8881 a         4674 c         5782 g         7642 t
ORIGIN      
        1 tgacagaagg gggcaacttc aagaaataag aagaaattta cgaaaattgt ttctcaaatt
       61 tttgtaaatt ttcgacttag ttttcgaaga agctattttt gtcacagcct ctctttttaa
      121 gtttagctat tagataatga tgtttagttt tggataaacg aaatcaattc ttcacccgac
      181 aaaccatagt ttacagcaat caatagtttt aaacgtgctt tttgactatt aatactgtta
      241 cagaaaataa tccccatttt ttctagttct tttcccccgc cttcataatc ataaacaggc
      301 tctgcgattc cattgaaaca acgagaaaca agtactacag ggattttttg gacaagaagc
      361 ttttccaaag cagctaaggc ttgtggaggg agattccccg cacctaaagc ttcgattacc
      421 aaaccgtcta ctttcgtatg ggcgatggct tccaataagt ccccttgcat cccagcatat
      481 gctttaacga tcgggatagt cccatctaca gcggaaatgt ccagtcgctt tgtttctaaa
      541 agttcctgca agaataaaat tcgattctta gttactaagc cgatgggacc caatgtaggt
      601 gttcggaaag tagcaacatt tgtggtatgg gtctttgtca cgtagcgtgc agaatggatc
      661 tcatcgttca tgaccaccaa aaccccctta tctagcgctt cttcacaact tgcgacacga
      721 atcgcacttt caaaattata aaggccgtca cttccaagtt cattacttga acgcattgcc
      781 ccagtcaaaa caattggtaa tttgttgcca atcgttgtat caagaaaaaa agcagtttct
      841 tctaatgtat ctgtaccgtg tgtgatcacc actccggata tacctgatgc ataagcggac
      901 aagatacgtt cttttaatac caacatgtga gaaggcatga tgtgtggtga gggaagattg
      961 aagatatctt caactaataa ttctacatgc ttggggattg tgatttctgc atctaataat
     1021 gggtttgcca catcaggact aacaccgccc gtgagcatgt tttctttcat ggcgatcgtt
     1081 ccgccggtgt gtagtacaag tatttttttc attttacaga cttccttacg aataattttg
     1141 catagccgtt aaatcttatt gtatactata taaaagagtt ttgctatcag tgaagaataa
     1201 cgaatagcct aacttatcat aaaaaagctg accttattaa aatggaaaag actcaacctt
     1261 gacctcctaa tacaatggag tatctggttg agtttctttt acaagaaagc agccaaggaa
     1321 atcaatcacg aaggtggccg gaaatgatca gaatcggatt aacatcgttt aaagaacatg
     1381 acaaattaac tggtaaaaag aataacactc tttatgaata tgcagggttt ctacccttag
     1441 tggaattaga tactgcctat tatggaattc cccgaaagtc ctcagttgag aattggataa
     1501 gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta tcaaggaatc agcggtcaag
     1561 gagactggaa gccttattat cagaccgaag aagagatgat tgcgcatttt ttagatgtta
     1621 tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt agtccaattc ccctcatcat
     1681 tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact tagaaagtgg tttgagggtt
     1741 atcctgtttc tatagaattg cgggattatt cttggtacag cgatgaattt cttgaacgta
     1801 caagaaactt tatgtctaga gccgcttttt ctttagccat catcgatgag ccacagttgc
     1861 tgaatacaac gattcctttt gatccatttg tcaccaatcc gaactttgct ctttttcgat
     1921 tccatggtag gaatgcgcaa ggatggcaag caaatgataa agagtggcgt aaaaaaagaa
     1981 cgttatatcg ttattcagaa aaagaactgc aagaactagc acatgaagtc aaagtagttg
     2041 attctaagac aaaagaggta ggtgtgattt ttaataataa ttctggtggt gatgcggctg
     2101 aaaatgcatt agcactgcaa aaaatattaa atatcaagcc agatggactc aatccaaccc
     2161 aaatggatct gttttaaggg cttattttat tcaagaaagg ggcgaagtct atgatcaaag
     2221 ctattttttt tgatattgac ggaacattgg tcaccaatcg ttcaaaagcg ttggaatcaa
     2281 caaaacaagc aattgaatat gctcggaaaa acggcatttt atgcggtgta gcaacggggc
     2341 gcagcccagt aaagatcaaa gaaatcattg atgaattgga attagatatg tatgtcgtgt
     2401 ataatggtca actggttttt actgcagatc gcacgattat cgaccaccct tttgaacaaa
     2461 aagtattgga acatattgtt gaatttgcag atgagaacca tcgacaaatc gtttttggtg
     2521 cgagaaatcg gttagatgga tcaacgacga tgctattagg acaatcgatt tttatcaagc
     2581 gactagtcag ctttttaccg cgtaaattcc cggtacgtct gatgaaaaaa atcctccagg
     2641 tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt agagatatta aaagaaccaa
     2701 tttatcaatg tatattgctc agtccggagt cagaacagaa aaagttagaa catcgctttc
     2761 ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga tatcatccca aaaggtggtt
     2821 caaaattact aggcatccaa gctttcgcaa atgctgtcga aattgaaatg gaagaaatta
     2881 tggcattcgg cgatcattac aatgatatcg agatgctgaa aggtgtagga attggagtgg
     2941 ccatggggaa tgctcagatc gaagtaaaac atgctgcaga ctttgtgact caatcaaatg
     3001 aagaagatgg gatatacgaa ggactgaaac acttcggtct gatttattag tcaatgaaag
     3061 aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa aagtagaaac attccacaaa
     3121 acttttgatc caagaagacc agagataccc acggctttta cagaaaatga agctcataca
     3181 cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg cagctacttc acctaataaa
     3241 gaacaactgc atgaagctgt tatgctgctg cacgaagcat tagataaagc agaagaaaaa
     3301 atacttggta aaacacagtg gggaagtgat ccgctggttg aacaagtaga tgcactgaca
     3361 gatatccttt actttactta tggttctttt tcattactag gaatagtgcc tgatcgaata
     3421 ttcgaaatcg tccatcaggc aaatatgggg aaattatttc cagatggaaa acctcattat
     3481 gaccctgtaa caaataaagt gttgaaacct gaaaactggc agatggaata tgctccagaa
     3541 cctaaaatcg cggcagaaat ccaacgtcag attcaagaag cgattgctaa gaagaaatcc
     3601 gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag agtttcacta tttttgttat
     3661 ctttatacga tggaaaaaca aaagattttc gtttaagtta gtgaatgcat gaaaatagta
     3721 tgctataatc tagaatagat gtcttttgca cgagagacgt attatttgga tttatgccaa
     3781 gcagaacgcc atctgttgca taaaagatgc atactgaaaa cggaggaacc ataaatggga
     3841 aataatgtta tcatcggaat cgtcattgca atcattatag tcgccattgt cgtctttttg
     3901 gttagttact tgatgaagaa aaaaaaccaa gatcgattgg atgccttaga aaaacgcaag
     3961 gaagaattat ttgacttgcc agtgattgaa gaagtcgatg atgttaaaaa aatgcatctg
     4021 gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa aatggaatga catatctact
     4081 tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg aaaacctcaa tgaaagtttt
     4141 cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag ctacaatgga ccaaatggag
     4201 aaacaagtga cggaaatccg tgaaggtcta aaggaattgc gggaaacgga agaacgcaac
     4261 tcactagctg ttcagcaggc acttgacgtc tatgaagaac tgaaagaaaa agtcagagaa
     4321 gacgagcatg aatacggacc agctcttttt gaattgcaaa aacagatcaa agtgatcgaa
     4381 aaagaattta cgcagtttgt gactttaaac acttctggag accctgttga agcccgcgag
     4441 gttttggata aagcagaaaa acatacgtat gaagtggaag ctctgatgaa aaaaattcca
     4501 ccgctgtatg aggagctcca taccactttc cctgaacaac ttgaagaaat cagtgatact
     4561 tacgagaaat tgatggaaga acaatatgta ttcccagaag aagatcttgc tgaagatatc
     4621 gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc ttgaaaaaac agaagttgaa
     4681 acggtcgaat ttgaaaaccg tgaaacagct gatttgattg attcgttata tgatatctta
     4741 gaacgggaaa tggaagctca gagatatgta aaaactaacc aatcaacgat tgcagagtat
     4801 atcaaacata caacaaagaa taaccgtcaa ttattgattg agctggatca cacatctcaa
     4861 agctatacat tgaaccacaa tgaaattggc cgtgtacgag gtttccaaac cgaagtggaa
     4921 gaaatggaac gtcaaaatga acagatgatt ccacagatcc gtcaacacga aattccttat
     4981 tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct tagaagatat cgaaaagcaa
     5041 caagtagaga tcgacgactc attacatgaa ttgagaaaag gcgaaaaaga agcgcaagaa
     5101 aagatcgata cattcgaatt caaattgcgc agtttgaaac gttttgtaga aaaacaacgt
     5161 ttgcctggct tgccgaatga ttacctagaa ttcttttttg ttgctactga ccgtattgaa
     5221 gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg aagaggtcaa tcgtttagtt
     5281 gctctatgtg aagaagatct agaattatta gataagaaga cacatgattt agtagatgca
     5341 gcagcattaa cggagcaaat gctccaatat gcaaatcgtt atcgtcatac acacgaagaa
     5401 gtgagagaag caattgataa aagctactat ttattcaata aagaatatca ctatcaagaa
     5461 gcattagacg aaatcggtac agcattagag cgagtagaac ctggtgcttt caaacgcatc
     5521 gaagattttt attttaatca tccagactta gtataaagca aaaaagttag taaaccagca
     5581 agagcaagac ggattattgt cttgttcttg tctttttttg tatatttgaa aattgaaaca
     5641 agtgaaaatt tcacctgctc ttgaaagcac cttggacaac cgttataata gaaaagaatt
     5701 tcaaaaaaga ggggtttctt atgatatatt ttgataacag tgcaacaacc ccaatctttc
     5761 cgcaaagctt agatgcatat gtaaaaacca gtcagcggat tattgggaat ccatcaagct
     5821 tgcatgattt aggcaatcaa gctaaccggt tgttgcagca agcaagaaag cagatcgctg
     5881 atttgatcca tgtagaacca gaagaaatct attttacttc aggcggaaca gaaggagata
     5941 actgggtatt aaaaggaacg atgatcgaaa aaagagaata tgggaatcat atgatcattt
     6001 caggagtcga acatccggcc gtcagcgaga cagcagaaca attaaaagaa ttaggaatcg
     6061 aagtaagtat cgccccagtg gataaacgcg gatttgtacg tgtcgacgaa ttgaaagaat
     6121 taataagaaa agagacagta ttagtctctg ttatggctgt gaataatgaa gtaggatcga
     6181 ttcagcccat ccaggaaatc agtgatcttt tggatgattt tcctaaaatt catttccatg
     6241 tagatgctgt tcaggcgatt ggaaaagtac cattctcgca atggttgaca gaaagagttg
     6301 attttgcaac attttcagct cataaattcc atggcccacg gggaactggt ttcatttatt
     6361 ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg agggcaagaa agaaatcagc
     6421 gaagtgggac agaaaatgtt ccaggtattg tggctatggt taaagcttta cgtttatcat
     6481 tagagataaa ggaacaaaaa cctgagcaga cacaacgctt aaaagtagct ctaatcaatg
     6541 cgttgaagac gtatgagaaa gtgacgattt tttcagaagg aactgactat gcaccgcata
     6601 ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct cgtccatgcc ttagaagaaa
     6661 aacaaatatt tgtttctact actagtgcct gctcctcacg taaacatatg gcaagcagta
     6721 cattgcacgc aatgcatgta ccaaatgatc tggctacatc tgctgttcga atcagcttag
     6781 atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat tattttcaag cagctttata
     6841 agaaattttc tgcaatcaat taaaagagga gtgttcttgt gaaatacagt gaaattatgg
     6901 tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa aagctttatc atgcaactag
     6961 cacaaaatgt cagacaagct ttgaaagact ttccagaaat caaaatccat gctgatcggg
     7021 accggatgca tcttttattg aatggcgcag acgggcagct agttattcca aaactagcaa
     7081 aaatatttgg gatccaaaat ttctctccaa gtattcgtgt agaaaaagat gtcgaagttt
     7141 tgaaacaagc tgttcaagac atcatgaaag aaatttatac tgggaaagaa acatttaaaa
     7201 ttatcgctaa aagaagcgat caccaatttg aattgacaag taatgaattg aatcaaacat
     7261 taggtaatgc tgtttttgat attttcccgc atatccaagt tcaaatgcgt caaccagata
     7321 ttccattacg tgtagagatt cggcgtgatg gcgcttatct atcgtatgaa acgatcaagg
     7381 gagctggagg attgcccgta ggaacaagcg gaaaaggaat gcttatgctt tctgggggga
     7441 ttgattctcc tgtagcaggg tactttgcca tgaagcgggg agtggaaatt gaagctgttc
     7501 attttgctag tccgccgtat acaagtgaac aagcactgca aaaagcaaaa gatctaacag
     7561 ccaaactagc accttatgtg ggaacgatcc attttatcga agtgccattt accgagattc
     7621 aagaagaaat caaaaagtca gtgccacaag gatattggat gacaattact cgtcgaatga
     7681 tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg tttagtcatt ttgaatggtg
     7741 aatcattagg tcaagtggct tcacaaactt tgcaaagtat ggtggctatc aatgaagtaa
     7801 cgaatacgcc aatcattcgg cctgtagcga caatggataa acttgaaatc attgaagtag
     7861 cagaacagat cgatacattt aatctttcta tccagccttt tgaagattgc tgtacgattt
     7921 ttgctcctcc acagccaaaa acacgccctc aactggaaaa ggtccatcaa tatgaagagc
     7981 gtttagcaat agatgaaatg atccaacgag cactagctgg cttgaagatg gaagagatac
     8041 aagcagaatc gtctaatgaa aaaactgaag aattttcaga attgctataa gaggttatga
     8101 aaatagtgtt ttgaccagag tattagagaa acaaaacgaa aaaatagctc catgtatttt
     8161 accgtttttg aactaatgtc gaagattagc tgattgaata ccatttatca agaagttatg
     8221 gaaaagccgg ttctttttta ctaactgaaa aataaaattt tatttagaga tcaagctaca
     8281 gttgataaat gatataaaaa agagaatttc ccatgaaata gcgaggttgt gacagaagtg
     8341 ggcagcttca agaaataaga aggaatttac gaaaattgct tttcaaattt ttgtgaaatt
     8401 tcggcttatt tttcgaagaa gttacttctg gaacaccgtg tatcagaggt tgcgaaaaag
     8461 ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata ttttacgttt
     8521 ggttgagctt attaccgcag atgcaagctt ttgaacaccg ttttatttgt ctcacaccct
     8581 tttccttcct acttgtaacc ttgctcccgt gttagaaaca tgttacacta aatatgtgaa
     8641 attattaaca aggagcgtcg ttatgcgaga aaaaacagat acactgccaa aagcaactgc
     8701 gaaaagatta cctttatatt tacgctactt gaagatgctg gatgactcag gtataagtag
     8761 aatcaaatca aatgagttca gcgaaatcac ccaaattcct tctgcaacga tccgcagaga
     8821 cttctcgcaa ttaggcgagc taggcagaag cggatatggc tatgatgtcc cttttcttat
     8881 cgatgtattc aataatatcc ttaatactaa agaagaaaaa cgaatcgcat tagtaggata
     8941 tggaaatctt ggcaaagcat tgaaacacaa caattttaga agaaatgaaa atctaaatat
     9001 cgtgtgcgtc tttgataatg atccagcact gatcaatcga gtaattgatg gagaaatgat
     9061 ctatccgatt gatcgtttcg cggagatagc caaagcaaaa aatgtgacag tagcaatctc
     9121 tacggtacca agtaaatatt cacaatctgc tattgatgaa attgtcaaag gaaatgtaac
     9181 cgctatactt aattttgcgc ctgacagagt aactgtccca gcttatgtca atgttcaata
     9241 cattgatttg acaaccgagt tgcagacgct tatttacttt gatgagaatt actcagaagt
     9301 tttttcttag gaaaatttga gtaggcttat tgatcttgcg agaactcgtc gtaattcttt
     9361 caaaaatttt ttagtactaa atgaagtggt tttttcacga ccacttgttg actaaaaaaa
     9421 aaaaccattg tagaatgaag cataacgctg aacaagagga gtaactggca cattcattct
     9481 tagagagaga actatctgct gaaaggttct caatgacaac cagtgaaggt agcttgggag
     9541 tttctgtctt gaatggagta gagatagacg agtcgtgatc gttaactcac ttgagaggca
     9601 ataatgattg attattgcaa tttaggtggt accgcgtaaa tcatacgtcc tagaatacga
     9661 aagtatttta ggacgttttt tagtttaaag gaggaaaaca gcatgacgga agaaaataac
     9721 ctatcaacaa aatataatcc gcaagaagtt gaagctggac gttatcagca ttggttagaa
     9781 caagatttgt ttaaaccgag cggagataaa aaagcaaaac cttattcaat cgtaatcccg
     9841 cccccaaatg ttactggaaa acttcattta gggcatgctt gggatacgac cttgcaagat
     9901 atgctgattc gtcaaaagag aatgcaagga tatgatactt tatggctgcc tggtatggac
     9961 catgcaggaa tcgcgacaca agcaaaagta gaagcgaagc ttagagagca agggatttct
    10021 cgttatgatc taggtcgtga aaagttcatc gatcaagtgt gggaatggaa agaagagtat
    10081 gcgagccaca tccgcgaaca gtgggctaaa atgggcttat ctttagatta cagtagggaa
    10141 cgttttacat tagatgatgg attatctgaa gcagtacgta aagtttttgt taccttatac
    10201 gaaaaaggtt tgatttatcg tggtgaatat atcatcaact gggatcctca agcacgaaca
    10261 gctctatctg atatcgaagt tattcataaa gatatcgaag gcgcctttta tcatatgagc
    10321 tatgaactag cagatggatc aggcgtagta gaaattgcga caacccgtcc agaaacgatg
    10381 ttgggagata cggcgattgc tgttcatcca gaagacgagc gttatcaagc gctcatcggc
    10441 aaaaaagtga ttctaccttt agtcaataaa caaatcccaa tcattgcaga tgaatatgta
    10501 gatatggaat tcgggacagg tgtagtaaaa atcactccag cacatgatcc aaacgacttt
    10561 gaggtaggta atcgtcatga tcttccacgc gtaaatgtta tggatgatga tggtacgatg
    10621 aatgaactag caggaaaata tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg
    10681 caagatctga aagaaatcgg tcgattgatt aaaatcgata cgatgatcca cagtgtgggt
    10741 cattccgagc ggacaggtgt cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa
    10801 atggcgccgt tagcgaaaaa agccattgat aaccaagaaa cagatcaagc agtggaattc
    10861 ttccctccaa gattcaatca aacgttttta cgttggatgg aaaatgtcca tgattgggta
    10921 atctcacgac aattatggtg gggacatcaa attcctgcat ggtatcacaa agaaacaggt
    10981 gaaatgtatg tagggatgga agcaccagcg gatagtgaaa actgggtaca agatcctgat
    11041 gttttagaca cttggtttag ttcagctttg tggcctttct caacaatggg ctggccagat
    11101 acggaaagcg aagattataa gcgatacttc cctactagca ctttagtaac cggttatgac
    11161 atcattttct tctgggtaag cagaatgatt ttccaaagct tggaatttac tggcgaacgt
    11221 ccatttgaga atgtcttgat tcatggattg attcgtgacg aacaagggcg gaagatgagt
    11281 aaatcattag gtaatggaat cgatccgatg gaagtaatcg aaaagtatgg agcagatgct
    11341 ctccgctggt tcttatcgaa tggttctgca ccaggacaag atgtccgctt cagctatgaa
    11401 aaaatggatg cttcttggaa ctttatcaac aaaatctgga atgcttcacg tttcgttatc
    11461 atgaatatcg aaggaatgac cgtagaagag atcgatttat ctggagaaaa atcagtggct
    11521 gaccggtgga tccttactcg tttgaatgaa acgatcgaac gagtaactga gctatttgat
    11581 cgttttgaat ttggtgaagc aggtcgacaa ctgtataact ttatctggga tgatttttgt
    11641 gactggtaca tcgaaatgag taaagaagta ctttatggag atgatgctgt ttcaaaacaa
    11701 accacccgta gcatcttagt ccatacactt gatcagatcc tacgtttatt gcatccaatc
    11761 atgccattcg ttacagaaga aatatgggaa catattcctc atcagggaaa ttctctagta
    11821 gtggcagaat atcctgttgt acgtccagag tttgatgatg aaacagcttc taaagggatg
    11881 gaagtattga aagaattgat tcgagccgtt cgaaatattc gttcagaagt gaatacacca
    11941 ttgtctaaac caattacgtt gctaattaaa acaaacgatg ctaaaatcga tcaattttta
    12001 gttgaaaata caagctatat cgaacgcttc tgtaatcccg aagagttgac gatatcaagt
    12061 gagatcactg cgccagatct agcgatgtca gctgttctga caggtgcaga aatcttcttg
    12121 ccgttagcag gattgatcaa tattgaagaa gaaatcaaac gattagaaaa agaactggaa
    12181 aagtggacaa gcgaagtaaa acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct
    12241 aacgcgccgg aagaagtagt agaggcagag cgtgcaaaag aaaaagatta tttagagaag
    12301 caaacggctg tcaaagaacg aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag
    12361 agagtgtgaa atcagctagc tattactaaa agctagctga ttgaacatcg ttacaggaaa
    12421 gagtttgagc ctcactctta gcttcgattc ataaggagat gtgacataaa ttttgtcgca
    12481 tctctttttt agtctactct tattatgtag gagaaggagg gaggtggatc atgtatcaag
    12541 agtcaataaa gtagttgata aaatcgttcc tctctctgtt tcttttctct ttgtcatact
    12601 tttatttttc ttatgtgttc tatttgttat aataaaagag aaatggggga attgttgatg
    12661 acgattgaag aagcaataga atggatccat agtcgattac catttggttc acgtcctggc
    12721 cttgaccgag tcgaagcttt attagaagtg gtagggcatc cagagagaaa agtaaaaacg
    12781 atccatatcg ctggaacaaa cggaaaaggt tctactgtag cttatttgag aagcctatta
    12841 gaagaggctg ggctgacagt aggtacattt acttcacctt atatcgaaac gttcaatgaa
    12901 cggatcgcaa tcaatggcaa aagcattcca gacaatgatt tgatccagct tgtaaaaaaa
    12961 tatcagccac tgatcaaaca acttgatcaa tttgaagcag tagctggtat tactgaattc
    13021 gagacattaa cagcaatggc atttgattat tttttgatga agaaagtgga cgtagcgatt
    13081 atcgaagttg gcttaggcgg cttattggat tctaccaatg tagctgtacc aatgctgacg
    13141 ggaataacaa cgattggact tgatcatacc gaaatacttg gagatacaat tgaagaaatc
    13201 gctgcacaaa aagctggaat catcaaacaa ggcgttccag tagtaacagg aaatattgcc
    13261 ccagaagctt ttactgtcat ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta
    13321 tacggggaaa attataaaat aaactatctg catcctgatg agatgtgggg agaagtattt
    13381 gaatttttta atgaaatagg aaagatccct caattaacca ctccgctttt agggcaacat
    13441 caagctgaaa atgcaggagt agccatcgaa ttgtattttg tttactgtca attggaaaag
    13501 ctgcctttcc gagaaaaaga tgttttgaag ggattgaaga aatctcgatg gccaggaaga
    13561 atggagcgtt tgagtaacga gccattagtt gttttagatg gtgcccataa tacgcatgca
    13621 atgaaccggc ttgttgagaa tttgaaaaaa gaattcaaag gatatcaaat caacattttg
    13681 ttttccgcct tacgaactaa aaatgttaga gaaatgctag aacgattgca gcaagtacca
    13741 aatgtccata tttatttgac gacctttgaa catccaaaag ccatcgaatt aagtgaagta
    13801 tcagatttag caagtgagag agtatcaatc gtttcattgt ggcaattcgg attagcagat
    13861 atattggaaa agatgacaaa tgaagacgta ttagttgtga ccggttcact ttatttcatt
    13921 tcagaagttc gccatttatt aatacaatta ggaggagatc atgaagtata atgcagtgat
    13981 ttttgacatg gatggtttgc tgttcgacac agagttagtg tattacgaag ccacacaagt
    14041 agtagcagac aagatgggtt ttccttatga taaagacctg tatttaaaat tcttaggtgt
    14101 ttcagatgaa gaagtatggg cgaattatca tatgattttc tcggaatttg gcaaaacaag
    14161 tgtacaaaga ttcatagatg attcatacca ggaaacgctt gaacgttttt cgcaaggagc
    14221 ggtacaaatg aagcccggtg tgttggaact cctctctttt ttagaagaac atcagattcc
    14281 caaagttgtc gcttccagta accaacgagc agtcattgaa cttttattga agaagaacaa
    14341 tctgtttgaa cgatttgaga cgattgtttc tgcagaaaat gttcaacgag caaaaccaga
    14401 cccagaaatt ttcttgttag ctaatcagaa gctaggtaca gcgaagaaag aaacattgat
    14461 tttagaagat tcacaaaatg gtattttagc agcttctgcg gctgagatcc cggtagtcat
    14521 ggtaccagat ctgcttccac catcccaaga attgatcaaa aaaacagctg gggttgtttc
    14581 ttctttacat gaaatccctg cattgttaaa ataaaaatcc atctcttttc gtatcttatt
    14641 ttagataaga tagaaaggag atgttttttt gggaaaacga ttgatcaaag aagtacctac
    14701 tagctcttta ccaagggaac gtatggaaat ttatggcgcg gaagctttat cggatcagga
    14761 gcttttggca atcttactgc gtaccggtca acacccttat agtgtgatgt cgattgctgg
    14821 aaatctgtta aaaacgtttg gaggactggc ctctttacga caggctactt tgcatgagtt
    14881 agaagagatt cgagggatag gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt
    14941 aggtcgacga attcagacgg atcatgaaag agcggctcca ccggttcgta ccagctacga
    15001 attggcccag caattgattc tggaaatgaa agatcacaaa caagaacatt tggtttgtat
    15061 ttatttggac acgaagaatc aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa
    15121 tcagtcagtc gctcatccta gagaaatttt ccattatgct gtacgttatt gtgcagcaag
    15181 gatcgtttta gcgcataatc atccttctgg taatgttatt ccatcgcagc aggatatgaa
    15241 ttttaccaaa aggatacaaa agtgtggaga aatgatgggg atcactgtac tggaccactt
    15301 gatcatcgga cgcaaacgat attttagttt gagagaagaa ggaatgatgg aagagaagta
    15361 agttgctttc ttgcaaaaaa aatgaagcaa gtgtacaatt atattgtaat tttgtttggg
    15421 aatagaagtt gaaacatctg ttgcacctct atcgtacacg agcaatgtta caatttaaat
    15481 tagtgctttt ggcacgagga ggatattatt atggaaaacg gaacagtaaa atggttcaac
    15541 gctgaaaaag gatttggatt tatctcacgt gaagatggaa gcgacgtatt cgttcacttc
    15601 tcagctatcc aaggtgacgg attcaaaaca ttagaagaag gtcaagcagt gactttcgac
    15661 gttgaagaat cagaccgcgg cccacaagct gcaaacgttg taaaagctta attttatctg
    15721 taaatcaaat tgatggtaga catcaatttt tacgagagaa caaagcgagt aacaaaaata
    15781 cctgccacaa gagacaattt ctcttgtggc aggtattttt ttgtatgaaa aagtaaataa
    15841 atttaagagg ctatgaaaga gccaccttgt atcaaggaat aagaacaaac aaaaatggct
    15901 tctcatgttt ttcatttgtt ttctcttata ttcaggacaa ggtgctgatt tcataacctc
    15961 ttatttattc ttcatctggt gtcattacca ttggcaaaat cattggatgt ctttctgtac
    16021 gttcgaataa gaaaggctga agcgcgccag tcatcgcttc tttcaattta tattctgaac
    16081 agtcaggtcc attcaaacat tctcttaaag cgtggaataa gattcgctga gcttcattga
    16141 tcatttctcc tgattctcgc atgtagacaa atccacgaga gagaatatct ggaccagcca
    16201 agatttcttt tttcttgata tccactgtcg cgacagctaa tacaagacct tcttctgaaa
    16261 gcaggtgacg gtcacgtaaa acgacattac caatatcacc gatcccgcta ccatcgatat
    16321 acacatctcc agccacaaaa tgcccagcag gacgcgcaga atcgctagtt aacgctaaaa
    16381 catcgccatt ttccatgatg aagcagtttt ctgcaggaac acctacatct tgtgctagag
    16441 aagcatgtac tttcaacatg cggaattctc cgtggacagg aataaaatat tttggcttca
    16501 tcaaacgaag cattaatttt tgttcttctt gtccaccgtg accggaagta tggatgttat
    16561 tgatttttcc gtgaatcact tctgcaccag cttcagacag caaattgatc aaatgattga
    16621 cacttgttgt attaccagga ataggtgaac ttgaaaagat cactgtatcg tctggctgga
    16681 tttggatttg acggtgagta ccattcgcga tgcggctcaa cgcggccatc gcttctcctt
    16741 gagaacctgt acataggatc atcgtttcat tggcaggcaa gcgattgatt tcgtgtgcat
    16801 ctacaaatgt gccgtctgga actttgatat atcctaaacg ttgaccgttg acgatggcat
    16861 tttccatgct tcgtccgaaa acagcgattt tacgaccagt tgccacagcg gcatctgctg
    16921 cttgctgcaa acggaagatg ttagaagcaa agctggcaaa aatgatacgt ccttcgattt
    16981 ttgaaaagat cttcaaaata gaatgcccaa tcgttttttc tgatttagta aatgttggaa
    17041 tctcagcatt tgtactatca gataaaaggc acaagactcc ttcttccccg atacgagcca
    17101 ttttatgcaa gtttgctggt tcgccaacag gagtgaaatc gaatttgaag tctccagtcg
    17161 ctacgatatt tccagaaggt gttttcacca cgatccctaa tgcatctggg atactatggg
    17221 tcgttctgaa aaagctgaca gaagtctttc taaaacggat gatcgtatct tcattgatct
    17281 catggagctc tgcatctcga agcaagccat gctcatctaa tttgtttgtg atcaacgcta
    17341 atgcgagagg accagcatag ataggaatat ttgcttgttt cagcaagtag ggtattccgc
    17401 cgatgtggtc ttcgtgcccg tgagtaatca ccaaggcttt tactttgtgt aagttttgta
    17461 tgatgtagct gtagtcggga atgacatagt caattccaag taaatcgtct tccgggaatt
    17521 tgatcccggc atcaatcaaa atgatttcat cttggaattg taccccatac gtatttttcc
    17581 cgatttcacc taatccacca atagcaaaga cgcctgtctc gttatttttg atggaaagtt
    17641 tcatattaaa actccactaa tgagaaatca gcattttctt tttcgtactc tagatgactt
    17701 ccttctagaa gttgaacgaa ttcaatattg tatggggtat tttcttccac acgttggcga
    17761 gcgtctacat cgctttttgc ctcaatgtaa agagattttg tttcttcccg tttagggttg
    17821 cgtacttttg tttcttgata ataaacttta tagatcattg ttttgtctcc tttatctgcg
    17881 taaagcagtg atttagctag ttgatttttt tagacaacac aaaacgaggg gacaagccac
    17941 ctggtttttc atatgctttg actagaatac tggattcgag tcaaagagac aagcctcgtt
    18001 tttgcttatc tgttcggata ttttttcaac cgtaccttct gtataaaaaa tatacaacaa
    18061 ttaattgtta ttctagcata aaacagcaaa taagtataga aatcaactag attgaaagaa
    18121 aaaatcaatg tttatcatcc cgctataatc ataatgactt ttttatgaga gaagtgttct
    18181 attaataggc tcgttgtgct atcattttta gtaatgaaaa tgtaacaaaa gattaaaagg
    18241 agatgagaaa gtgaaactaa tgtggcatta taccatgcgt tataagaaat tcttgttttt
    18301 caatttcata tgcgtatttg gttttatttt gattgaatta ggacttccta cgattttagc
    18361 acgtatgatc gatgtaggaa tccgcaatga tgattttgat tatgtgaagc aacaagggat
    18421 atttatgatc gttattactg taatcggtat tattttgaat atcttgttag gttattttgg
    18481 ttcgaggatt acgactaata tcgtagcaga tattcgagat gatttgttcg aaaagatcca
    18541 aagttattcg catcaggaat acgaaaatct aggcgtatct tcattgatca ctcgtacaac
    18601 aaacgatgct tatcagatca tgttgttcct gcaaaatatt ttacgtatcg gttttatggc
    18661 accaatgatg ttcattgtca gtttgtacat ggtcatgcgt acaagtgtga cactcagttt
    18721 gtatgtagta ggtgcattac ctttactatt attggcagtc gtagcgattg ctaaggtgtc
    18781 tgaaccgtta tctaaaaagc aacaaaaaaa cttagataaa atcaacagta tcttgcggga
    18841 aaatctttca ggattgcgtg tgattcgtgc attcgtcaat gaaaagtttg aagaaaaacg
    18901 ttttgctgta gtcaatgaga attatgcttc tagttcaaaa agcttattcc gtttgatggc
    18961 tgttgcacaa ccgggttttt atttcttatt taatatcgta atggtattga tcatctggag
    19021 cggggcagtg caaatcgatg caggcgagct tcgtgtagga gatttgattg cgtttatcga
    19081 gtatatattc catgccttgt tctcattcat gttatttgct agtgtgttca tgatgtatcc
    19141 aagagcagct gtttcagctg gacgtatcca agaagcccta gatgccaaac cttcgattgt
    19201 agaagatcca aatggtatat ctgatacaaa aactaaaggg tatatcgaat ttcaaaatgt
    19261 aacatttgct tatccaggcc attcgcaaga gccagtgata cgaaatgtca gttttacagc
    19321 ctctccagga gagaccgttg cttttattgg aagtaccggt agcggaaaat ctacgttgat
    19381 ccaattgatc cctcgctttt atgatgtgag tgaaggaaag atcttgatcg atggagtaga
    19441 cgtgagagat tatcggctaa gcaagttgcg agataagatt gggtttattc cgcaaaaagc
    19501 gctgctattt actggaacga ttgcagataa tttgcgatac gggaaagaag atgctacgca
    19561 agaagaaatg gaacgtgcag ctgagattgc tcaagcttca gaattcattt ctcgaaaacc
    19621 agatggattt aatgaacatt tatcagaagg tggttccaat ttttcaggtg gacaaaaaca
    19681 acgattggca atagccagag ccatcatacg ccgtccggaa atctatattt ttgatgacag
    19741 cttttctgca ttagattatc aaacggatgc gaagttacgt gctcgtctga aaaaagaaac
    19801 gacagaatct actgtattga tcgtagcaca acgtgtggga acgatcatgc atgcagataa
    19861 gatcatcgta ttaaatgaag gagaagtcgt cggtatgggg acgcatcggg agttgttgga
    19921 aaaatgcccg atttattatg atattgccgc ttctcaatta tcaaaggagg aattagcatg
    19981 aaaaaaacct tttcttcctt aaaacgttta gctcgatata tcagaccgta tcgtgagact
    20041 tttcttttag tgatcctatt tacagttctg acagtggctt tcaacgtggc tttgccatat
    20101 gttacaggac ttcctactac agaaatcagc cgtaatattg ccaatggcga atcaatcaat
    20161 ttttcttata tattcaaatg tctcatttgg atcacagttg ttggaatagg gtattgtatt
    20221 tcgcaattac tttctggtgt tttgatgaca aatgtcgttc aaagtgccat gcatgatttg
    20281 agaagagaca tagaagaaaa aatcaatcgt cttccggttt catatttcga taaaaaccag
    20341 caaggaaata ttttgtctcg tgtgacgaat gatgtagatg cagtcagcgg tgctttacaa
    20401 caagctttta ttggtatcgt aaatgcgata ttgggaatta cgttggctgc agcaatgatg
    20461 ttttatatcc agccgatgat ggcgctgatc tctatgatca tgattccaac atcgatctgg
    20521 atctcaaaac gagtgatcaa tgcttcgcag aaatattttc aaagcatgca gaattctctt
    20581 ggtgagctaa acggctacgt ccaagaaaat atgacaggtt ttagtgtatt gaaagtatat
    20641 ggacgcgaaa aagaaacatt ggaaggattc aaaaaagtta atcacagctt gaaatacttt
    20701 gggtttcgtg cagcattcat ttctggcttg atgatgcctc tagttcaatt aactgcctat
    20761 gctacttata ttggtatggc tgttctagga agcttttatg ttattactgg ggcgatcgtt
    20821 gtcggtcaac tacaagcatt catccaatat atttggcaaa tcagtcagcc aatggggaac
    20881 gtgacacaat tgtcttcagt tttgcaaagt gcatcagcgg caacgaaacg tgtattcgag
    20941 attttagatg agccagaaga aaaagaaaac aaagtagatg cttcactgcc agaaacgatt
    21001 ttaggagatg tgacattcga tcatgtcgat tttgcatatg atccgaagaa accattaatc
    21061 caagatttga attttgaagt gaaagctggt caaaccgtag ctgtggtcgg tccgaccggt
    21121 gccggcaaga cgacattgat caatctattg atgcgttttt atgacgtaga caaaggagca
    21181 ataaggattg atggaatcga caccaaatca atgagtcgca gtgatgtgcg ttcgctattt
    21241 ggtatggtct tgcaagatgc atggctttat caaggaacga ttgcagataa tatccgcttc
    21301 ggaaagcttg atgcgacaga ttatgaagta gttgatgctg cgaaaacagc aaacgtagat
    21361 cattttatcc gaacgatgcc agatggatat gatatggcca tcaattctga gggagacaat
    21421 gtgtctttag gacaaaaaca gttgctgacg attgcccgag cagtggtctc cgatcctaaa
    21481 atcttgattt tagatgaagc aacaagttca gtagacaccc gtttagaagc gttgattcaa
    21541 aaagcaatgg atcgcgtgat ggagggacgg acaagtttcg tcatcgctca ccgtttatcg
    21601 acaatccgtg atgcagatct gattctagtg atggatcaag gtcagatcat tgaaaaagga
    21661 acgcatgaga gtttattggc acaaggtgga ttttatgaaa aactgtataa cagccaattt
    21721 gctgaggaag cagaataagt catataagaa taaaacagtt taaaacttat tttttgttct
    21781 ctattcttga taatggtaaa gtaaagttat caagaaaagt gaggacacgg agatggaaca
    21841 gtttttaaat gaaaaaatta ctgaagccaa aagagaagtt gatcgtttaa cgcacgtctg
    21901 tcagacgaag ccttttgatt cagctgattt ttttgaaaat gagtttcaaa tcgaacgctt
    21961 ggcagcacaa atagaagtgt acgaattaat tttaaaaaaa gtaatagaag aaaatataga
    22021 tgtacaataa aaacaggtaa aacgtcagtt accagatact gtaaatagac atctggcctg
    22081 agcaggtttt acctgttttt tttcttatga aataaatata tgctatgata cctttattca
    22141 ataggattag tttctacaaa aaatgaaagg agaaaaataa atatggaacg gtttatagca
    22201 tcaaatgcca cagtgattgg tgatgtgaca ttgtcagaag atgtgacgat ctggtatcaa
    22261 gctgttttac gcggggacag taattggata aaaataggac aaagaacgaa tatccaagat
    22321 ggtacaatca tccatgtcga tcatgatgca cctgtagata ttgcggagaa tgtgacggta
    22381 ggacatcagt gcatgctaca tggctgtaca atcgaaaaag gggcgctgat cgggatgggg
    22441 accacgatat tgaatcatgc ggttattggt gagaatagtt tgattggtgc tggatcatta
    22501 gtaacagaag gaaaagttat tcctcctaat gtcttggctt ttggtcgccc agctaaagtg
    22561 atccgtcctt tgacaaaaga agaaatacaa aaaaacaaag aaaacatcca gcattatgtc
    22621 gagataggtc ggatgcatgc gcaaggaaaa ttcaaggagc aaagataggc agtagcatcc
    22681 ataaaataaa aaaggatggt cagtttgagt tgaccatcct tttttatttg gtttagataa
    22741 gagagcttaa aaaagcttcc tttgaaacag attaatttaa tgaagaactg ttttccatga
    22801 cttcatcgat caatccatat gcttttgctt gttcagctgt catatagttg tcacgatctg
    22861 tatctttttc aatcacttca agcggttgtc cagtgcgttc agctaaaatt ttgtttaagc
    22921 gttcgcgtgt ttgcaaaata tggcgtgccg caatttcgat ttcagttgct tgcccttgag
    22981 cgccgccaag aggttgatgg atcatgatct cagcatttgg taaagcaaat cgtttacctt
    23041 ttgtaccagc tgtcagcaag aagcttccca ttgaagcagc catacccata acaattgttt
    23101 ggacatctgc tttgacaaag ttcattgtgt cgtaaatagc catacctgct gtaactgaac
    23161 caccaggaga attgatataa agatagatat ctttttcaga atcttgagcg tctaagaaaa
    23221 gcaattgggc gatgatcgaa ttagcaagat catctgttac ttggccgctt aacatgatga
    23281 tacggtcttt caataaacgt gagtagatat cgtaagctct ttcacctcgt gaagattgtt
    23341 caatgactgt aggaattaaa ttcataatag agttcctcct tgatttcgtt tagaaatagt
    23401 gtaccataat ttagtttcta atacctaaaa tgtagtatac atttttggtc aaaaaaggtc
    23461 aaataaaaaa cttaggcaaa ttagtcgcat aaacccgctt tatggtatga taagcagaga
    23521 aacaaaacaa ggaggaacaa cagattgata gttggtatta ctggccttat cggagtgctg
    23581 acaggtgctg tagttgtcta tatcggcatg atactgcaaa tgcgtcactt ggaaaataaa
    23641 gaacagcgta gacgtgaatt ggaattgaaa gtcaaagaaa tcgagacgct gaatcaatta
    23701 aataaaaaga tcaacgagat cttgcaaaaa cggacaacgc tgatggaaga atatgtcagc
    23761 tttgatgcat ttgatgattg ttatatcacg attgacgatt ttgcgtatct ccaatcattt
    23821 tcagcgcaaa atagttttta tctgccaaat tatattttag acgagctatt taagaatatc
    23881 tcccatcgaa aagtgatttt atctccagat gaaacaatga agattggtgg atatgcttat
    23941 aaaggcggac gcgtgatttt agagaatttt acggatagtc tgactgaaat gatcaatgaa
    24001 aaaaaagctc agttgaagac tatgacgcgc cagccgattg gtttcttctc gaaagatgat
    24061 gtttggagct gatattttga agcattgccg ttgcacatga gagcgtggta atgctttctt
    24121 tttttcttaa tgatacaata gaactgaacg gagtgaagaa agatgttaca aatctatgcc
    24181 catcgcggaa gcagcggcac ccacccagaa aatacgcttc ctgcatttgc agaagctgta
    24241 cgagtaggtg cagatggtat cgaactagat gtccatctaa gcaaagatgg ttatttgatc
    24301 gttatgcacg acgaagaagt ggaccggacg actaacggta aagggctgat tcgagaaaaa
    24361 acattggaag aactcaaaaa attgaatgca gggggcaaat tcagcaaaga atttccagca
    24421 gctaaagttc ctgaattgag agaagtgtta ggactgctcc tccaaaaaaa ttaccgaggg
    24481 atgctaatga ttgaaattaa aactgatcag tatgaatatc ccggtatcga agaaaaatta
    24541 gccgaaacat taaaaagtag agattggcct tttcaacatg catattgcag ctttcatttg
    24601 gattctttgg aaaaaatcgc attactggaa ccggagatac agctggactt attgatgagt
    24661 acttctgata aaaagatcca actagcagca gaaacccctt atatagaagg agtacatcca
    24721 aaaaatactt ggttgttcga acatccagtc caggcagctg attttccaaa agcaattcgt
    24781 gcatggacga ttaacgatga agaagaaatg gccaaatgtc aagaactcaa aatagatgga
    24841 ataataactg attttccgga gaaagctctt cggtggaaac aatcaacgaa aagtaggtaa
    24901 aatatggaaa aagtattagt gatcgtcggt ccgacagcag tcggtaaaac agcgctaagt
    24961 gtggaactcg cacaactatt tggaggagag atcatcagtg gtgattcatt gcaaatctat
    25021 aaaaatctgg atatcggtac agcaaaaatc aaagaagaag agatgaataa tgttccgcat
    25081 catttgattg atgtgatcga acctactcaa cgttactcag ccgttgattt tcagcattca
    25141 ggtcgacaat tgattaccga gattgcgaac cgagggcacc tacctatcat agctggtggt
    25201 acgggtctct atatccaatc ccttctatat gattatcagt taggagcgac agaagaagcg
    25261 aatcctgaga ttagaaaaaa gtatgaaacg ttagctgaaa caattggttc ccaagcatta
    25321 tggaatcagt tgaaagaaaa agacccggca gctgctgaga aaatccactg gaacaatcaa
    25381 cgcaaagtga taagagctct ggaagtttta gagacgacag gacatagtat cacggcacct
    25441 aaagaagagc caaagcaatt gtatgattac ctcatgattg gattagatac gaagcgtagt
    25501 attctttatg aacgaatcaa tcaaagagtc gatttgatgc tagcagaagg tttagtagca
    25561 gaagcgcgta aggtgtatga gcttggagaa gtccaggcca gtcaaggtat tggatataaa
    25621 gaatttttcc cttattttga agggaaagaa acattggaag aagcaacaga acaagtcaaa
    25681 ctacattcga gaagatacgc gaaacgtcag ttgacgtggt ttcgtaaccg tttggatgca
    25741 cagtggtttg atcttataag tgagccaaat caaaaaagtc agattgagca aacaatcaaa
    25801 aaatggctgg aggcggaata aaatggaaga aaaagtgatc atcgtagggg tagaaaccga
    25861 agcaaaccag cgttactttt cagagtcaat ggaagagcta gttcagctga cgaataccgc
    25921 atcaggagaa gtcgtattca caattacaca aaagagacca caagtggatc ggcaaacgat
    25981 tatcggtaaa gggaaattac aagaattagt tcaacaagcg gatgcgcatg aagcggattt
    26041 gatcatcttt aatcatgagc taactccgag acagagccaa ctgattaccg atgctgttgg
    26101 tcttccggta attgatcgcg tgcagcttat cttagatatt tttgcgatgc gtgctcgttc
    26161 gaaagaaggg aagctgcagg ttgaacttgc tcagttagaa tacctgctgc ctcgtttagt
    26221 agggcaaggg aaaacattat ctcgtcttgg cggtgggatc ggaacaagag ggccaggtga
    26281 aacaaagtta gaaacagacc gccgacatat acggaataaa atttttgctg ttaagaaaga
    26341 attgaaagaa gtagaagctc atcgagagcg caatcgccaa aaaagaaaaa atagcgagat
    26401 ttttcagatt ggacttattg gctacacgaa tgccgggaag tcaactatct tgaatctatt
    26461 gacacaagcg gatacttatt cgaaagatca attgtttgca acgttagatc cattgactaa
    26521 gagatggcgt tttgctgaag gatttgagat tactgtaacg gataccgtcg ggttcatcca
    26581 agacctgcct acgcaactga tcgatgcttt ccattctaca ttagaagaaa gtcaaaatat
    26641 ggatcttttg ctacatgtag tcgatgctag ttctccagat cgtattttac aggagcagac
    26701 tgtcttaaaa ttgatggatg aactgaatat gaaagaaatg ccgatactga ctgtttacaa
    26761 taaggcagac cagatcgacc cagcgatgtt cacgcctacg ctattcccaa atgtattgat
    26821 ttctactcag acgaaagaag gtaagatggc gttgatcgaa gcaatcaaac gtcagctgat
    26881 ggaactaatg accccttata cactttctgt gccaagcaac gaaggacaga aattaagcga
    26941 gttgagaaga caaacaatgg ttttggacga agcgtacat
//
LOCUS       DANKAY010000029        26839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000029 DANKAY010000000
VERSION     DANKAY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(104..1336)
                     /locus_tag="PWY98_001224"
     CDS             complement(104..1336)
                     /locus_tag="PWY98_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2036985.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="PWY98_001225"
     CDS             1591..2160
                     /locus_tag="PWY98_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2036986.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="PWY98_001226"
     CDS             complement(2338..2778)
                     /locus_tag="PWY98_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2036987.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="PWY98_001227"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="PWY98_001227"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2036988.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="PWY98_001228"
     CDS             3732..4655
                     /locus_tag="PWY98_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2036989.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="PWY98_001229"
     CDS             4731..5870
                     /locus_tag="PWY98_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2036990.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="PWY98_001230"
     CDS             5863..6663
                     /locus_tag="PWY98_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2036991.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="PWY98_001231"
     CDS             6663..7490
                     /locus_tag="PWY98_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2036992.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="PWY98_001232"
     CDS             7468..8202
                     /locus_tag="PWY98_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2036993.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="PWY98_001233"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="PWY98_001233"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2036994.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="PWY98_001234"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="PWY98_001234"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2036995.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="PWY98_001235"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="PWY98_001235"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2036996.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="PWY98_001236"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="PWY98_001236"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2036997.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="PWY98_001237"
     CDS             11787..13820
                     /locus_tag="PWY98_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2036998.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="PWY98_001238"
     CDS             13864..15144
                     /locus_tag="PWY98_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2036999.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="PWY98_001239"
     CDS             15354..16160
                     /locus_tag="PWY98_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037000.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="PWY98_001240"
     CDS             16172..17392
                     /locus_tag="PWY98_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037001.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="PWY98_001241"
     CDS             17382..18968
                     /locus_tag="PWY98_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2037002.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="PWY98_001242"
     CDS             19007..21145
                     /locus_tag="PWY98_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2037003.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="PWY98_001243"
     CDS             complement(21513..22496)
                     /locus_tag="PWY98_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2037004.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="PWY98_001244"
     CDS             22874..24265
                     /locus_tag="PWY98_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2037005.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="PWY98_001245"
     CDS             24281..25321
                     /locus_tag="PWY98_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2037006.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="PWY98_001246"
     CDS             25340..26425
                     /locus_tag="PWY98_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2037007.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26839
                     /locus_tag="PWY98_001247"
     CDS             26458..>26839
                     /locus_tag="PWY98_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2037008.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQI"
BASE COUNT         8611 a         4454 c         5449 g         8325 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttattttaaa aagaatagtt cagtggagtt tattcgtgga
    26821 atagatctag atcaaatta
//
LOCUS       DANKAY010000030        26707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000030 DANKAY010000000
VERSION     DANKAY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..249)
                     /locus_tag="PWY98_001248"
     CDS             complement(<1..249)
                     /locus_tag="PWY98_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037009.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKES"
     gene            complement(321..1601)
                     /locus_tag="PWY98_001249"
     CDS             complement(321..1601)
                     /locus_tag="PWY98_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDL2037010.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(1692..2144)
                     /locus_tag="PWY98_001250"
     CDS             complement(1692..2144)
                     /locus_tag="PWY98_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2037011.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2157..2567)
                     /locus_tag="PWY98_001251"
     CDS             complement(2157..2567)
                     /locus_tag="PWY98_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037012.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2842..3054
                     /locus_tag="PWY98_001252"
     CDS             2842..3054
                     /locus_tag="PWY98_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037013.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3057..3269
                     /locus_tag="PWY98_001253"
     CDS             3057..3269
                     /locus_tag="PWY98_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037014.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3409..3564
                     /locus_tag="PWY98_001254"
     CDS             3409..3564
                     /locus_tag="PWY98_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037015.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3561..3785)
                     /locus_tag="PWY98_001255"
     CDS             complement(3561..3785)
                     /locus_tag="PWY98_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2037016.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3863..4051
                     /locus_tag="PWY98_001256"
     CDS             3863..4051
                     /locus_tag="PWY98_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037017.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4048..4221
                     /locus_tag="PWY98_001257"
     CDS             4048..4221
                     /locus_tag="PWY98_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037018.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            4377..4712
                     /locus_tag="PWY98_001258"
     CDS             4377..4712
                     /locus_tag="PWY98_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037019.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            4945..5886
                     /locus_tag="PWY98_001259"
     CDS             4945..5886
                     /locus_tag="PWY98_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL2037020.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5888..6778
                     /locus_tag="PWY98_001260"
     CDS             5888..6778
                     /locus_tag="PWY98_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2037021.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6821..7621
                     /locus_tag="PWY98_001261"
     CDS             6821..7621
                     /locus_tag="PWY98_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2037022.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7636..8487
                     /locus_tag="PWY98_001262"
     CDS             7636..8487
                     /locus_tag="PWY98_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2037023.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8484..8687
                     /locus_tag="PWY98_001263"
     CDS             8484..8687
                     /locus_tag="PWY98_001263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037024.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIIY
                     RDTERFLTVLGNVYENPELLEVE"
     gene            8688..9149
                     /locus_tag="PWY98_001264"
     CDS             8688..9149
                     /locus_tag="PWY98_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2037025.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9175..9627
                     /locus_tag="PWY98_001265"
     CDS             9175..9627
                     /locus_tag="PWY98_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037026.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9733..9927
                     /locus_tag="PWY98_001266"
     CDS             9733..9927
                     /locus_tag="PWY98_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037027.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            9958..10251
                     /locus_tag="PWY98_001267"
     CDS             9958..10251
                     /locus_tag="PWY98_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037028.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            10281..10721
                     /locus_tag="PWY98_001268"
     CDS             10281..10721
                     /locus_tag="PWY98_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2037029.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            10796..11263
                     /locus_tag="PWY98_001269"
     CDS             10796..11263
                     /locus_tag="PWY98_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL2037030.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            12010..12240
                     /locus_tag="PWY98_001270"
     CDS             12010..12240
                     /locus_tag="PWY98_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037031.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            12399..13232
                     /locus_tag="PWY98_001271"
     CDS             12399..13232
                     /locus_tag="PWY98_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2037032.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13225..14640
                     /locus_tag="PWY98_001272"
     CDS             13225..14640
                     /locus_tag="PWY98_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2037033.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14652..16181
                     /locus_tag="PWY98_001273"
     CDS             14652..16181
                     /locus_tag="PWY98_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2037034.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16099..17151
                     /locus_tag="PWY98_001274"
     CDS             16099..17151
                     /locus_tag="PWY98_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2037035.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17153..17470
                     /locus_tag="PWY98_001275"
     CDS             17153..17470
                     /locus_tag="PWY98_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037036.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17597..18220
                     /locus_tag="PWY98_001276"
     CDS             17597..18220
                     /locus_tag="PWY98_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2037037.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18235..19125
                     /locus_tag="PWY98_001277"
     CDS             18235..19125
                     /locus_tag="PWY98_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL2037038.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19148..19363
                     /locus_tag="PWY98_001278"
     CDS             19148..19363
                     /locus_tag="PWY98_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037039.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19375..19716
                     /locus_tag="PWY98_001279"
     CDS             19375..19716
                     /locus_tag="PWY98_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037040.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19716..20087
                     /locus_tag="PWY98_001280"
     CDS             19716..20087
                     /locus_tag="PWY98_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037041.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20080..20475
                     /locus_tag="PWY98_001281"
     CDS             20080..20475
                     /locus_tag="PWY98_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2037042.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20477..20854
                     /locus_tag="PWY98_001282"
     CDS             20477..20854
                     /locus_tag="PWY98_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037043.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20855..21463
                     /locus_tag="PWY98_001283"
     CDS             20855..21463
                     /locus_tag="PWY98_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2037044.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21463..21804
                     /locus_tag="PWY98_001284"
     CDS             21463..21804
                     /locus_tag="PWY98_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037045.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22046..24835
                     /locus_tag="PWY98_001285"
     CDS             22046..24835
                     /locus_tag="PWY98_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2037046.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            24825..25565
                     /locus_tag="PWY98_001286"
     CDS             24825..25565
                     /locus_tag="PWY98_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2037047.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            25562..>26707
                     /locus_tag="PWY98_001287"
     CDS             25562..>26707
                     /locus_tag="PWY98_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2037048.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMI"
BASE COUNT         9526 a         3859 c         5281 g         8041 t
ORIGIN      
        1 actttctttt acagttggct tgtatttttc atcccattta gtttttaatt ctccaaaagt
       61 tagtggtaat tcttcatttc gttttatttt ttctgcgatt ttttcattca aaagtaagtt
      121 agcttgattc caagcttggc gtgaattgct agataaagta acggacactt ttctcttttt
      181 ttcagttaag ggatccgtat aacgttcggt gtacttaaat tttttgtcat ttattttttc
      241 taaccacatt atttctattc ctttcgaata tacgttccta ttaattgtta aaagaaaaag
      301 cccgaaggct ttttatatat ttaatctttt gacaattcta aagatcgtct atattgttca
      361 gctttagcta attttgtaaa tgttactgtt ccattataat tttctaaaac aactttctct
      421 atttcgtcta gtgaaacttt gaaaaattct tttctttgat taattttatt aacttcttgt
      481 tttctaaaat gattatgcaa tttagtttcc aaagacggag cgtcctctga aaaaatcatc
      541 gcatgaacgt caaattcaaa tggaaccgat gcactactca attcttttat tctatccata
      601 ggttctaatc ttctagtcat accaatttta tatatgtctt caccaaaaga cccaatatta
      661 ctaattacat acacataacc ggctctagta tttaattctc tttgtaatac attttcacgt
      721 ataacttcta attcttttaa tttttcttcc agttcttgaa ttttatttgc atataattct
      781 ttttccacat ctgcattagc tttttgcata tatgcgatta atttggaaat ttcagaattg
      841 aattgacgtt gttctttatc aatacgtttc ttttcacgat caatttctct acgaactttt
      901 tcctcttcta tcatctgttc ttttattgct ttttgttgtt ctttttcttc ggtaattttc
      961 actaaataag agtaatgaag ttctagttct tgaagtttaa actctaaaag acccttcctt
     1021 aattcagcat tgtcattctt gaataaatta ttaattttat caaatatttt tatgattttt
     1081 tccctagtcg agtctatatt agaaatagtt agtttattga gtaatccttt cacttctgaa
     1141 tcaaatggaa tgagcatttt tttctttaaa ttatttttct ctcgtttatt taaaataaga
     1201 gggacagaaa cagcatcgtc aataatatat tccttttctt ttaattttaa caaagaaagc
     1261 ttatttttta tttcagaaga tgaagtttct tctatatttg aaacgataag tgaagaatag
     1321 acatttttta atgtttcatc atacttttga tttatttcat catttaaatc ttctttctct
     1381 ttttttaata aaataatatc attttttaac atagtaattt gtttatttaa atctgatagt
     1441 gtttcttcct tacttaatat aaatctttct ctatcatgta atttatttag tttttcatca
     1501 tttttttgtt ctaattcata agcttctttt tttattgtgt tagctttcga cagtttagtg
     1561 attcctataa ttaataaaat aagtccaatc aataataaca ttcaaaactc ctcatttctt
     1621 tgatataatt attgtagagt ttactcagaa atgaggaaag agtccatgtg aaagcatggg
     1681 cttttttatt tttaatgttg taatactaaa tgttcaagat tatgaggaag ccctaaatat
     1741 tcaataattt ggaactgact catatcagat aaatcttgtt catctattgt tctagttaac
     1801 atttcaattg caaacatatt agcttcagct tctataccat aaatcataca actaaggttg
     1861 tttgctctaa aagctgctgt gcaaatacct ttatggagtc taacgtgggc tatttcatgc
     1921 cataatacaa atcttttcat agaaggtgat aggttttgat taattaatat cacataacat
     1981 cttttattgt gtatcgtttg tcctaatgtt ttattcccta aatcagtttc aattaaatct
     2041 atatcagaat atttgattat tttatatgga ttaatcgcat cagatccaaa ctctacaaat
     2101 aatttttcta tttcttctaa tatccagtat ttgtttaaat gcataccaaa gcccacctat
     2161 tgacgatatt ttttaggagt gaattttttc tttgctttaa ctttggttaa ggcaagtgca
     2221 agttgaatgg catcgtataa tttttcctta tcttcatcgt ctaaaggttc accataataa
     2281 tttgtttcag catctgaatt caagccttca agtaattttt cagcttgtaa agcaatatct
     2341 tgtttatctt tttctgttaa atcataataa tggcgtttat cagtacgtcc taaaagataa
     2401 tcagttgata cattaaagta atctgcaact ttttgtattt tatctccaga tggagtactt
     2461 ttattccatc tagacatgct tccatttccg aaatttaact ttctttcaag ttctgccaac
     2521 gtcattgaac gttcttttaa taagattttt atacggtcta atagcatagg atatgcattc
     2581 ctttcaaaat accgacaaaa aaagtatttt tctgcaaatt atctatttac aaatagagaa
     2641 tttgcagtta tactagtttt gtaagcaaga tatttacggc atacaaagac taaaagcaat
     2701 aaactaatag tctccccaga catcagtaag ctcgcttttt gtgcgctgta ttttgtatgc
     2761 ttacataata gcatatttgc agtttttgtc aaacttatta gacaaaaata ttagaaaata
     2821 ttcgataagg gggtatgaag catgacttta aaacagcaag tattaattgc tttagtaaag
     2881 aaaggttgga gtcaaagaga gttagctcga cgaatgggta tatcaattac ttatttacga
     2941 gatatcttca tcgaaaaaag aaaacctaag gaacgtttaa aacaaatcga agaacttttg
     3001 gatatcaagt tagaagtaga ttctaatgat cagccatcag aacaggaggc gtaaaaatgg
     3061 tacaacgttt gagcaaagcg gagtttaaag agaaatataa gtattctgaa agtacttatc
     3121 aaagaagaat gaaggaattt aaaaaatcac gttttagtga ggggtataac gcagttactt
     3181 ccaaagaaat aacaattgat gtcgatttat atgaacagtt tagaacatgg cgttctaaaa
     3241 atcgatttag agttagagct gtttgttaat agctaaaaaa tctaaattca ccacttctga
     3301 aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc cacgtttttt cattttggca
     3361 actcctcttc ttggaggtgg tgacggcgag agacgaagga gggatgacat gaaggaagaa
     3421 tcaaaaatag ggattttgaa tgagaacaat tacacaagat taattattga aacagaagaa
     3481 gggaaaaaag tagcagaagt tacattgacc gatgcaactc ctgctgacgg ttatcgtatt
     3541 cgattaactc ctaaatatga ttaacctttt ggtgggtgtg gatcgtttcc gtgactatca
     3601 cggctttgaa tttttccatc tctgccatga ataaatagct cagatccttg atttttagaa
     3661 atctctctag cgatttttac tgcttctgat tttgtattag tatgtgcagt cgctttagaa
     3721 tttccagcac ctttgacatt ccaaccacca ttggaattag gtacaacatg ttgatttttt
     3781 cccatagttt tcacctccat ttctatagaa atattttatc aaatagaaat ggagaaattc
     3841 aataaacgaa aggagaagtg atatggcata taccttgcaa caagaacatc aaattctcgg
     3901 tttgattaaa caacgcagga aacaattaca agatgaccgt gcagcgctta gaaaagccga
     3961 tgagctatca gataggcaag ctgaactaat tgcttctgaa cttgaggatt tgagaatgct
     4021 agaaataaaa aatagggaga ttagattatg aagaagacag acacactttt tataggattc
     4081 attttggggt tattagtgat tgtagcgcac caaagtatta tcgggggaag cttgttcgca
     4141 gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa ggagcaacta tggcacgaga
     4201 agaagcgcta aaaatcggta aagtgattgc tgataatcgg tggggaaata gccgccctat
     4261 tattttaagc aagcaacata tcgaaaagca aaaagcatgg caacaaataa aaaagtgact
     4321 ctgccagcca gcaaagagtc acatacaaat ttataaaact aggagaatta tatcacatga
     4381 ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga atgccaaaag caaggtgttt
     4441 ctattatttg tacagcccaa aaagaagggg aacttaaaag tctcgtttat ggtgagacaa
     4501 cagaaatttt actttgtctt gctatgcaag aagaacattt agatgaaaat ctcccattgt
     4561 cagcacatat tatgcgtaga attgctgtgg atgcttacga acaagcgaaa aatgaagaag
     4621 aaaatcaacc ttcaaatcat acttttgtta ttaataacaa ggaagattta gctgatgtga
     4681 tgactcgtat tctcaagggg gaatttcaat gatgaatgaa aaaacaattg atctactcat
     4741 tattttattt ttcacgttag tgattgtagc gaaaagttat gtaatctttg gcttgttatt
     4801 cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg
     4861 agttagcaaa tcttgacaac tatttaactg atcctgatta tgctaaacca ccatatgaag
     4921 caccaattga tgaggaggat gaagatgagt aaatctacct tagaaatgag ccatcaagaa
     4981 tggcttgaag accgtaagaa aggcatcgga ggttcggatg ttggaacaat tttaggattg
     5041 aataaatgga aatctcctta tcaactatgg ctagaaaaaa caggacaagt agtacttgaa
     5101 gaatcaggaa gtgagcccgc ttattggggt aatgttttag aagaagtggt tgctaaagag
     5161 tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc aagtatttga acatccatta
     5221 catccatttt taagagcaaa tattgatcgg gaagtagtgg gagaaaatgc cattctggaa
     5281 tgcaaaacag ccaatcaatt tctcggtaaa gaatgggaag gcgaagaggt accgcttagc
     5341 tatctctgtc aagttcaaca ttacatgaac gttctaaaca aagactattg ttacatcgct
     5401 gtcttaatcg gtggtcaaaa atttatctgg aagcggattg aacgagatca agagctgatc
     5461 gatacaatca ctgaacaatt agtagagttt tgggaaacaa acgttcttgg aggtatcgag
     5521 ccaattattg atggtagtca agcgacagct gatttcttga aagagaagta tgcagatgtt
     5581 gaagacgttc aaacgacgtt accaattcat ttcgatgaac tagttgaaca gaaaaacgaa
     5641 ctcaaacgga ctaagaaaga aattgaatca gctatccgtc aagtagacaa cgagattatc
     5701 agcgagctag gtaaacgtga agccagtatc ggtatcactc aaaaaaacat catcagttgg
     5761 aaatttgtcc gtacgagacg tatgaactcg aagaaactag cagagaaata tccagatgtc
     5821 gcaaatgatg aagagattta taacgttact gaatcaagaa ggctaaccga aagggagatc
     5881 aaataatatg gcaacaaatg aatcgttaaa aaatcaattg gcagcaaagc cacagaaaca
     5941 agttgcacca ggtcaattag gacttaaagc tttgatgaat acaccaacaa tgagaaagaa
     6001 gttcgaagag gtgcttcatg acaatgctaa tgcttttatg tcgaatgtta tgactcttgt
     6061 atctaatgac agttatcttg cagatagtga accgatgtct atcatgagtg gtgcgttaac
     6121 tgctgcaaca ttaaatcttg ggctagataa gaatttaggt tatgcatatt tagttccatt
     6181 caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa gctcaattca tgcttggcta
     6241 taaaggatat atccaattag cccaacgatc aggtaaatac aaagcattaa atgtgatcga
     6301 agtttacgaa ggagaactaa aaagctggaa ccgactgaca gaagagtttg agtttgatcc
     6361 aaatggtaga acgtctgatg aagtcattgg atatgttggc tatttcgagt tactgaatgg
     6421 attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa
     6481 taaagatcgc gataagacaa agttaagtgg tgtgtgggca tctgattaca atgcaatggc
     6541 acgaaaaact gttttgagaa atcttctttc taaatgggga atcttgtcca tcgaaatgca
     6601 agaagccacc acatcggatg agagagtcca aagggttcaa gaagacggca gcattattgc
     6661 tgaaacagaa gttgaagaag atattcctga aagaaaagaa gcagaggtta tatctgaaga
     6721 aaatgaagat gtacaaactg gattatttga tgcatctaat ccgccgttaa acaaataatg
     6781 agggagcttt ctcccttgct tttctagagg agaaatacga atgaacacag gatatgtcaa
     6841 attacatcga aaagtgatga attcattcgt ttggaccaat ccttatatgt ataaattatg
     6901 gaacttgtgt ttgatgaaag cgagtcatga gaatcgcaag atacttttta atggaaaaga
     6961 gatacaagtg aacagcggag aattcgtcac agggcgcgat gctatcacat ctgagatgaa
     7021 cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct gtatggagat ggctaaaaca
     7081 atttgaaaaa aacggaatgt tgaacatcaa atcaactacg aaatacagcg ttatatcaat
     7141 aaaaaactgg tctttgtacc aaagcagtga acaacaaatg aacatcaaac gcacaacaag
     7201 tgaacaacaa gtgcacacaa acaagaatga aaagaattat aagaatgaaa agaataataa
     7261 aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac accgactcag tttactacat
     7321 tctcgcggag gagttattca aacagatttg tcagaatcag gaaatcaaaa agccgaatct
     7381 gcaaagttgg gcagatgaca ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca
     7441 ggtacgggga atgattgaat ggagtcagca caatgtgttt tgggcatcga atattttatc
     7501 tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg caagcaaatc gtgattataa
     7561 aacaaaacaa actaaaacgc ttgaatacga gaaatttagc acagatgagt tgcctatttg
     7621 agaggaggcg taggcatgga aactgttggg gaaatcatgg aaaagctgat acagaaagtg
     7681 cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag
     7741 aaccctgatg gctcagaacg ttgtaagcca acctgtatgc aatgtgggta taaagcattg
     7801 cgagttcagg aagacttaca aacagaacga atttacaacg agagcctgaa agcaagagca
     7861 atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag cgttgtttga ttgcacattg
     7921 cagaattatc aaattgtcga tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt
     7981 gttaattcag tcttgttagg aaatccaagt caccttgttt taactggaaa acaaggaaca
     8041 ggtaaaagtc acctagcgat ggcagcggct tgggaagtct tgaagcgatc aaactacgat
     8101 aaaaaaatct tatttatcgg gttacaggaa atgctggatc aaatcaaatt ttcgtacaac
     8161 aatcctgaac tcagaaaaac gattgaggga tcgttgattg cagatatcaa aacagcggat
     8221 ttagtcatca ttgatgatat cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg
     8281 tttggcataa acacgctaaa ttcgttcttg gatgcacgac agaacctagc aacgattatc
     8341 acaacgaact tgcttggtga agaactgaaa aaagcttacg gtacgagaac aatatcaaga
     8401 atgtttgtca actctgatgg atttacgatg gtattttctc aaacagcaga caagcgcata
     8461 aaaccagtca aaggagcgat cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc
     8521 agataatgtt gcaaattata ggtgatcgtg acatggacga attatctcaa caagagagtc
     8581 gaatggttcg gaagataagt aagctaatta tttatcggga tacagaaaga tttctcactg
     8641 ttttaggtaa cgtctacgag aatccagagt tattggaggt agagtaattg ccaaaaatat
     8701 tagatgcatg ttgcggtagt agattgttct ggtttgataa gaacaattct gacgtaacat
     8761 ttatggattg tcgtcagcag tatgaagaat tatcaacagg tcatgtgatc aatgttgatc
     8821 cagatgtggt cgcagacttt cgcgatatgc cttttgaaga tgatgtgttc gatatggttg
     8881 tatttgaccc gccacactta agacatgtcg gcgaaaactc atggcttgcc aaaaaatatg
     8941 gaaaattaga cgaactttgg ccagaagaca tcaggcaagg ttttgcagag tgtatgcgag
     9001 ttttacgacc ttcaggaaca ttaattttta agtggaacga ggaacagata ccactttctg
     9061 atgtgttaga ggctattgga gaacagccat tgtttggtaa taaacgaagc aaaacacatt
     9121 ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca aaggaggaaa agtaatgaag
     9181 attgtattaa ataaatgttt tggtggcttt gggttgagcc atgaagcaaa aatggaaatt
     9241 tttaaaagaa aaaatattga agtattccca tacatcaata actttagcta tgactcagat
     9301 gatgaataca cgagatacac aggccaaaag ctaggttcga tggattttat ctattatttc
     9361 aaaaaagacc cgaaaatcga caaagttaca ggaatttata gcgaaattga ccgactgtac
     9421 ggaattgctg atgattccag tttttcaagt gattcaaaca gaggtgataa ggacctcgtt
     9481 gctgttgtcg aaaaactagg aagtgaggcc agtggaccat atgcaagtct taaagtcgtt
     9541 gatatacccg atggggctga gtgggaaata agtgattatg acggcgttga aactgcacat
     9601 tatggttttc aaacaggtag tatttagtcc actaatgaca actaaagagg aggcagaagg
     9661 atgagtgaag atgaatactt tgaacagtag tctattaacg acagagattt ttaactggaa
     9721 aggggtgtag tcatggacgc atcggaagta gtaaataaaa ttaatagtct caaagaggtt
     9781 ttcggagacg tcgaagtagt gataaacgca caaggctatg acaacgaatt ttttaagcag
     9841 attaccgaca ttagcattca gcaaggaatg gaagacgaag acggaaactt tatcgatgaa
     9901 gtagccattt tagtgacttg cgagtaagta attgacagct aaagaggagg aagcggaatg
     9961 aaactaaaag acggatttta ctccagcggc cacggcatcg gcggtttaat gctagatatg
    10021 ccgacaaaga atcctaaaac acgtaagaaa ccaaaattca aagtcggtga catggttcgc
    10081 tgcgaagcag aaggattcat ctatccattt cgtggatatg tagaacacgt ctataatcac
    10141 tcagcaatca ttcgcattga aaacacgatg gaatgtgata agtggttagc gaaaagcaaa
    10201 gagaatttag cagtggtgag attggtggac atggaagtta taaaatctta attttgcttt
    10261 tttacaacta tcctacaaca atgagcgtta ttccacaact ggaaggagaa gaggcaattg
    10321 tgaacatttt agagaatata gatattaagc aaacaaggaa aaatgctaga cgattactaa
    10381 aaagatacag aaatttagaa cgtttggtcg gaccagtgaa aatagacttt tccgtgatga
    10441 ctgttaccaa aaatttgaaa ttcacaattg acagtcaaaa cgaagaaatc attgaagcga
    10501 taagtactcg agattcggtt atcgaagcat taacacggct aagtagaatc catttccagg
    10561 tactttatta tagttattgt tttcctaata agatgtcaat gtatcaaata ggagaaaaat
    10621 taggatattc cgatagaact atcgagagaa tgaaggcagt agctttggtt gaatttgctg
    10681 aggcgtataa atcaggagaa ctcatttcac gtacaaaata aaaaaagtcg gattcctccg
    10741 actatgaata atatttccga cataagtatt atatcataat tgggggaatc agaggatggt
    10801 acttttcgat gtaaagaaat atgagacacc aagcgcgaaa gatgttgata tggaacgcac
    10861 aaaacataat gtcgctgtgt ttctttcagc atatctatca gctagatgta gagtagggca
    10921 acatcgagaa ccaaaagtga cagcatctta ttccttggtt ccaccttcta cagctgatca
    10981 tatatttgaa gcagaaagaa tgctgatcga taaagaagaa gcacaggaag aatttgagta
    11041 tctgcataaa ttgtttgttc gaggctattc agcgatacag catccgcata agcctgatgt
    11101 gactgaaagg cgcaagaaga tattctatga tcgttatatc aatggtctgc ccatttatgt
    11161 aactgctcaa aggaataata ctagcgaaga atcggttaaa gtagaatcaa acagaattat
    11221 catccaattt gcttcatcgt tagaactggt tgctttcaag tagccagttt ttacactttt
    11281 tatacccttt tattaccaat ttggtttcca ttttatacct tttttatacc aatcacttac
    11341 ctattcaacg ttgtattatg atagtgtcga aaaattagga aacaggatcg acaaaataaa
    11401 atgtaaggga ggaaatctcc ctcatcgttt aattaagctt cgataggcag caacggaaat
    11461 attaagaata aggatgtgaa tttcaactcc ttctaaattg ttcttattat ctatcatccg
    11521 ttgctgtcta ttgttttttt aattattcac acgataacta aaggtaagtg aagagaaatg
    11581 attccattaa taatttcaat ttttgcgctt tgtcttaatg tatatatgat tggatttaaa
    11641 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
    11701 aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat
    11761 tccttgccag tgactttggc aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa
    11821 tttcgtacgc gttttgtgca tcgtgtaggt tgctattaca taactggtta ggttagattg
    11881 agatttggga ttcggtacaa atgaatcgtc aaatgtctca agcacaggat cggaaacgtc
    11941 cctgcctgtg cattacatat tagatcactc tttgagtggt ctttttcttt acataaagga
    12001 ggctacataa tgagaaacta ctggtatgta tcgctaacta atgaatatcc tcgaaccatt
    12061 gatgattgtt cagtgcgtgt tgtgcgttct gtacaaatca aagggaagta ctctattgtc
    12121 gaaatgggga gagaagcaga accatgtgag attgatgcgt gcaagctcgt ttattgcggt
    12181 catggtttct atgatgaacc aaacattcaa aataatatta acaagaattt gagggattag
    12241 aatgcaagaa atggcttaaa aatctccaat caggacaaat caagttagac aaagtttccg
    12301 atttaaagat attgattgaa gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa
    12361 aactcaacct aaacagaatg tgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa
    12421 gacgtgatga agccaaaaga atttggttag aatccaacgg agaaaagcag ttaaaggaaa
    12481 ttgcatctga attaaatgtt tcagattctc aggttagaaa atggaaatcg caagacaaat
    12541 ggagcgctga attgattagt aacgttacca atggcaaaag taacgttact aatcaaggtg
    12601 gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg aaatagccga gcttctccgc
    12661 cagtgggtaa taaaaacgct ttgaaaacag gtgagtatga aacaatattt tttgacacgt
    12721 taaccgataa tgagaaggac atctattcta gtttgaatga tgatccttct tttgctttgt
    12781 ccgaagaaat acgacttctt aaaataagac aacttcgaat gatgaaaaga atccaacaag
    12841 ctgaagctgg actaaatgat gaagaagtcg aacgattgca gcagctaaga aagattaaaa
    12901 ctccgattga aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg caagatgtgc
    12961 agattagcag aaaaaaacat cgcaaaattg atgatattct ttcaattgaa gattcattga
    13021 ctcggattag caaccagtta gctaaagcca tcaagcaaat gaatgaactt tatatgaatg
    13081 aatacagaac tgatttaatt aaagctcaga ctgataagat ccaagctgag acaaatgaaa
    13141 ttggcggaaa taattcaggt gaagaaatag aagaatggaa acaggcagtt ttaaatgccg
    13201 caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc tgatattggt
    13261 tctgccattg attattacta tgataaacca gtagcttttt gccaagatat tttgcatttg
    13321 aatcctgatg aatggcaaga aaatgtttta aatgatttag ctgaattttc aaaggtttct
    13381 gttcgttctg gtcaaggagt tggaaaaaca gcattagaag caggagcaat actttggttc
    13441 ttaacgtgtc gaccctacgc taaagtaata gcaacagctc cgacaatgaa gcaactttac
    13501 gatgtacttt gggcagaggt agctaaatgg ttaaatgata gcttgatcaa aaacttactg
    13561 aagtggacaa agaccaaaat ttatatggtt ggtgattcag agcgttggtt tgctacggct
    13621 agaacagcga ctaaaccaga aagtatgcaa ggttttcacg aggaccatat gttgattgtg
    13681 gtagatgaag cttctggtgt gtctgatcca attatggaag ctattcttgg tacgctatca
    13741 ggttttgata ataagctgtt gatgtgtgga aaccccaata atattgaagg tgtcttttat
    13801 gattcccata attcagaccg tgataaatac agagttcata aagtatcaag ctatgatagt
    13861 aaacgtacaa acaaagacaa tatagaaatg attcttaaaa aatatggaaa agaaagtgat
    13921 gttgctcgtg tccgtatttt tggagaattt ccgaaaggtg cgttggattc atttatcagt
    13981 cttgaaacag ttgaattggc tacagaaaaa caaattagtg attctttagt caataaaaca
    14041 aaggttgctc atattggtgt cgacgtagct cgatatggtg atgattctac gattctcttt
    14101 cctagaattg ctaccagggc attggagtat gagaagtatt caaaacgtag caccatggaa
    14161 acaacaggat atgtcatcaa catggccaag aatctaatga gtcaatatcc gagtattgat
    14221 aaagtgatga ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa
    14281 gaacttatag aagacaaaca ttatcctttt gaggtgtttg gagtgaataa cggttcaaca
    14341 tcagaagacg atttttacga taatttaggt actcaactat ggggaaacat caaggaaatg
    14401 ttagaagaaa atatgacagc aaatcttaac ggagaacagc ctgttattga attgccttct
    14461 gatagttcgt taatcaaaga attaagtact cgcaaattca aaatgacaag tagaagtcgt
    14521 atacgtttag aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc cgatattgct
    14581 gacgcactgg ctttagcgtt ttatgagcca ccaagtcact atcaatttat tcaattttag
    14641 gaggtgagct tttgattagt acagtattat ctttagagag atataagaag ctacgtgtta
    14701 aatatgcaac acaaattgaa gatggaatgt ttgatccgaa tggttttata gaagatatga
    14761 aaccattttt tgctgatcgc gaaagaaaat atctagctta tactagcgaa aagaatgaaa
    14821 tagataatag accaaagcct aacactgata ttgtaaaagt taataataaa cttcatgctg
    14881 gtatgtattc aattgtcgta gaccaagcag tcaatcattt tactggtata cctatcaaat
    14941 gggattacga tgtatcggaa caaaaaagaa cactcattca aagattaaaa gataaattct
    15001 taaaaaatga tatagaactt ccgacagttc cagaagcttt taacaaatta acaagcaatc
    15061 ttgattccat gagattcgca atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg
    15121 gagtagcttt ccgattgcta gagcctgtgg aagaagatga tggttggaag ttaagagcaa
    15181 gtaatattga accttggaga gcagaaagat atggaaatgc cggaatctat atcaaagaaa
    15241 agtatgactc ttaccagaaa aaattttttc aagaaatgaa agttatgaca agaaataaaa
    15301 ttctgacata tgcttgctat ggtgatttga attttgttac aagcggaacg ttcaaaaaaa
    15361 ttgatgagac cgataaccct ttggggacga ttaccttggc agaatttaaa aataatacga
    15421 atcgttattg tgattttgaa gtagctgagg aaattggaga tgcaattgat cgtgctttgt
    15481 ctgatcaaca aaacgaaatc gaacaattta agcttgcgta catgcttgtt actggcacaa
    15541 caatgagcaa aggcactgct aaagaaatga tgaatcagct agggattatc aacttaaaag
    15601 cccctactgc aaaagctgaa tatgtcacaa aaaatttagc aaaagatttc aatgaatatc
    15661 atatggatct attgaaaaaa cagttttaca ccatttgtaa agcaatcgat tttaacgatg
    15721 aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt agcctagaag
    15781 cgaaaacaaa tactaaagag caatatttta gagaaggatt gaaagaatgc gcagaaacaa
    15841 ttgctgcttt tcttaagttt catgacaaag tagaaattga gcctgaaaaa attattttta
    15901 ctttctctag atctttgcca actgatttaa gttatttagc tgaagcttta cctaaacttg
    15961 caccatacgt atccaaacga actattcaga gccaaattcc atttgtaaca gatgttgatt
    16021 atgaaaatga aatgatggaa ttggaaagtg gaagtgctta tccagatagt gaatacaatt
    16081 ttggcggagg tggcaatagt gacgatagaa acgaagtatt ggaccaaacg tcgagaactg
    16141 gaggatcaag caaggctcaa acaagaaaat cagacactta aaaaattaac tagtgtattt
    16201 cctgaagcac tgaaagagat acaagcaaaa ctattatcac aagctgactt acacaatatc
    16261 acttatccag aaatgatgga gttttacagt acaagtaatc agaaaaaata tcgtgaatat
    16321 gtggaaaaaa attataagtc attaaaaatg tatgatgcaa aatacaaaga gtttatcgat
    16381 gaattttttc caccatttga ctatgcaaaa gtcaatcgct tattacaaat acgatcagat
    16441 gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg tgaatcaata tttttctgat
    16501 cgcttagagg aaattcttca aagaacatat tcttctaatg ctaatgtttt tgttcaactt
    16561 ttaaacgttg atataccaaa ttatttacca gaaaatgaaa ttaaacatta tttgaactat
    16621 ccatggtcag gaaagacatt ttcaagaaga ctttggggaa atatttcatc gcttgaacag
    16681 aaactctcta atgctattgt aaaaagtgtt gctagtggag aaggcgttat acacgcatta
    16741 aacaccatga gattagactc agaaatttgt gacatgttta agttagaaga atcaaagtat
    16801 aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca agatggtatt
    16861 gaaaaatcat atttagaaac aggtattgag gaatacaacg tattgactgc aaaagatgag
    16921 agagtttgcc ggatttgtgg aggaaaggca agtaagaatc cctataaact gaaagatgct
    16981 gtcataggtg aaaatcgagc acctttccat agtcgttgta gatgtacgga tgttcctaat
    17041 ttaccaaaat taggaaagga tattgatgaa gaatatgacc gtttatttgg cgatttatta
    17101 gatgagtttg cacatgattc ttttggaatt aatttgaaac ggaggaagta gaatgaaaga
    17161 tttttttgaa gcagtactaa caattaatgt aaatgctgat attgcagaag cctacaaaac
    17221 agctattgag tctgagaacc atcctaatgg cttgagagac cattggaatg gcaattatgc
    17281 ctacgtggtt attggcgatc aaactgttaa ttatcaagat aatactccag ttgataagaa
    17341 taccgttaat ttaacgattc aattattatc tcattcatta ccaaatttaa aagaaacagt
    17401 tgattggtat gaaaagatgg gatgtattgt tgttagaact gactacaaag aaggaaagtc
    17461 tagtaattag gcttttttat tttgtccgaa atgacgttaa actagcgcaa tactgggctt
    17521 ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc
    17581 gtggggcgaa aggagaatga gcatgaaaca caaatcatta atgccaatga atttgcaata
    17641 ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc
    17701 caatgaagaa gctcaaaaat ttattcagtc acaatctcaa agtgttgccg acaaacaatt
    17761 ggaagcttgg aaacaaaata acttagataa aattaaacag gataccatca aggaatatga
    17821 ggaatctaag aaaaacaagt cacctgagca aattcaactg gaaaaactac aagctgaatt
    17881 tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa gcatttgttg cagaacaaat
    17941 tgctggatta gaactagatg gagagttaaa agagtctatt tctcaattta tgctaaataa
    18001 tcttgttagt tcggatacag atttcactaa gaatgctgtt gaaggtttta caagtgtttt
    18061 gaatgcaatt aatgagaaac atgcagatgc attaaaagaa ctacaaatga agtctgcatt
    18121 tggtggaact caacaatcga ataaccaggt tcagcagaac aatgaaacat taacaaatcc
    18181 agaagaacaa ttaggacaaa ttcttcaaca atttaactag gagagtgaaa aattatgaaa
    18241 aaaacatctt taaataattt agagtatttg gatatttcac cagcgattaa tgctatgcaa
    18301 gtaccaaata cacctttttt aagctattta cttggtgctg gaaaaacaga gccagcaaac
    18361 tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg atgattcttc tgaaaaactt
    18421 gagggcggag aatatccaga tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa
    18481 atttttagaa aatcaacctc tgtatctggt acattagatg ctattaatgt gaatggagtc
    18541 ggaaatgaat taactaatca agtagcacta cgtggtatgg aaatgaaaat tgatttgaac
    18601 cgaaaattga ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat
    18661 gggattttga acttgatcaa ttcagcaaat aaggcagaaa cagccactgc gggtgcagta
    18721 acaagaaaag atatcgatac tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa
    18781 aaactatgct tgatttctcc agatatgcag gagttaatga ctgatgagtt agatggaaaa
    18841 tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg gattgcaaat tggaaatatc
    18901 gtgtctaatt acggtacagg tattgctcta ctagaaccat cattgccaaa aggaacaatt
    18961 gccgcaatcg atactaatta tgtgaaacta cgtccattac gtgaatggag agcagaagaa
    19021 cttgcaaaaa caacggattc tagacgtatc ggtcttgtag gtgaatactc tcttgaatac
    19081 aatgcttcaa actctggggc aattttaaat ttaaagtctg aataaaaggg agttagtact
    19141 cccttttttg ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt
    19201 cgtgaaatat agagtaggta aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg
    19261 tagatttatc acagcagatt caaataacga atttatcatt tctatagatg ataaagaagc
    19321 aattgcaatt ttggaagatg caattgatgt taatgaaatt taggaagtga tctgatggat
    19381 gaatcgctaa aaacggaaat cattgagtct acaaaagaag attttccaga tttgagtgaa
    19441 gaacgcataa ctaatttatt agaaataatt ttgctagaaa ttgaatcata caacacttgt
    19501 aaaaatgata tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata
    19561 ataaaaaatg aatcagaaaa aacagtatct tcagttagac gtggcgatac aacgattagt
    19621 tatgcttcaa caacaaatga tgttagtgag ttacttctag gctatggtga tttgatacga
    19681 agagttattg gttgtggagg attggagttt ttttaatgaa tgaagcagat attttagaaa
    19741 ctacttatga agatagttgt attattgaaa gactgacgga cattgaagat tctaatacaa
    19801 atattactat tcaagattac aaaaaagtat atgataatcc tatttcttgc gctctttcac
    19861 aaggtcaaat cgatggacta gcagtcatag aagatggaga gatggtaaat gtttcaactg
    19921 acacatataa attatttgtt catcctaaga ttaaactaaa aaaaggagat cgaataacaa
    19981 taactcaaaa ggccagtggc ttaatttttt ctctatttgc tactaagcct ttttactatc
    20041 ctagtcattg tgaagtgaat ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa
    20101 tcaaatgcag aaaagatcat tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa
    20161 ggaatatcct tcgttaacga ttcaatgaat aaagtcgtta gtttagctaa accattaact
    20221 cctgtaaaat caggtaattt aaggcgagga tatcgtgtag taaaagctag aaaactatca
    20281 agcggtcgaa ttgttggaga agttataaac aatgaacatt attttaaata tgtagaagaa
    20341 ggtcatagga ctaaaaatgg tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg
    20401 aatcttgcaa atatgtctta tattcctcga agatttaaac aaatggcaat aaaaatcgtt
    20461 aagaaaggaa agtaacatga aagatgaaat cattgctgca atcagtagca aattaaaaga
    20521 aatctatcct gatgggacaa tatatcttga ttcagttatg cagtcaacta aagattttta
    20581 tttcgtttta tcagtaatgg aatctggaac tgaaaatgta ggaattgata ttcaaaatgt
    20641 ttctttctta attgatattg cattgattga taataaccct aatagaaatt taattaatga
    20701 attagtctca cgttgtggga ctttttttaa tacgattaca atcgatgagc aaatattatt
    20761 tccaaaagcc tatttacctg atgaagcaga tggtgttcaa catattcgtt ttacattaga
    20821 atttccacaa tatattgaat ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg
    20881 aattatttct gtcgaaaaac ctacttggtt tcctttggaa gatgagacag ggaattttcc
    20941 ggtatatggt actgcgttgc caatgggaac agcagtaagt atcaaaccaa cagctaatta
    21001 tgaaacaaca caagattatg gggattcagt ggttcaagat caatttacag cttttggtgg
    21061 tgcagaagtt gaactggaag caaatggata tactcataaa gttttatcta caattactgg
    21121 agggaaaatt gttaaaggtg gtgccttgcg ttctggagaa gatattgcgc aagatggtgc
    21181 gtttgcatat agacgtaaaa aatcgaatgg gaaataccgt tatacggtct tttataaagg
    21241 acagtttgct ctagattcag atgaaacatc aacgattgaa ggaagtaaag taagttttac
    21301 tcatccaact tggaagggtt catttgtaga tgtaccagga cttggatata tgtattcagt
    21361 tgatgaagat gatgaaggcg tggataaagc aatgatcgga aattggttta caaaagtagc
    21421 aattccaatt gaagaagcag aactttcagg aggtacagaa taatgtcaaa atatcaaaca
    21481 acgattaaat taacgaaaaa agacgaagaa ggtaaatatg agcaagtaca atttaaatct
    21541 gctgaatttt taccaggaac agttgtagaa gatgctgcag gagttatgga agaaatgcaa
    21601 acagcgactg ataagcaatc agttaaaaaa gctttaagtc gtgcttattc atttattgca
    21661 gatactttat ttgagggaca atttactggt gaagattatt gcaaaggaat tgatgctcgt
    21721 gagattgcct cattgacagg aaaattattg aagtctgtta ctgcaggttt tgatgaaact
    21781 tatacagaga cgaaaaaaaa gtaagtgagg cgctcaagtc accttcattt aagtattcga
    21841 ttacttaccg agaattagat ataaagacgc aattactcga agcaggttgg acgttaccag
    21901 aaattgaacg tactgacttg gatgagttaa tgcgtctttt tgcttttaga gatgcggtaa
    21961 aagagcatga agatgttgag tactatgata atttcactca attttaggag gtgatacttt
    22021 gaataacgat gacctaattc tgaagatgat actagatgaa tctggtttta ctgccggtat
    22081 gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa
    22141 tagtggcagt tcattaggta gtatttggaa aatatttgct ggaagttttc tagctagcgg
    22201 agtaactaga atcgtaggag ctggctttga tctaatcaag ggttccataa gtggagcggt
    22261 tgatcgagta gatacgatga ataacgctct acgaaatttc caaaatatgg gatttagtaa
    22321 ttcagaaatt atgaagaata tcggaaagaa tgggctttta tctcaaggta ttcaaggact
    22381 tcctactgct ttgaatgatg cgataagtca tgttcaactt cttgcttctt ctacaggtga
    22441 tttaactcgt tcaactcaaa tatttaaagc tttaaatgat ggaattcttg gtttcggtgg
    22501 ttcaactgat caagttaatg aagctgttat tcaattatct caaagtttct caaacggaaa
    22561 agtagacgca caaacttgga actcaatgat caacgctcaa cttggaccta ctctttctgc
    22621 tattgctaaa aagatgggaa ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa
    22681 gatttctgtt gaagaattcc aaaatcaatt aatagaaatg gataccaaag gtggcggagg
    22741 acttaaatca ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa cctctataca
    22801 aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta atagaaggat taaataaagc
    22861 attagtagat tcagatttag gtggatttaa gggaattatt gataaagtgg gcagttcaat
    22921 ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa
    22981 tttatttaac gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc cctttcttgt
    23041 tcctgcagct accgcttttg gagcgttcat gtttcaactt aaaggtatac cagcaataat
    23101 aaaaagcttc aataatttta agaatgccat aattggcgtt ggaaattcat taaagattat
    23161 gggggcaata gctgctgcta atccatttgt tttaattatt ggtgcagtcg ttggtgcaat
    23221 agctgttttc ggttacttta tggcaaccaa tgaagagctt agaaacaagg ttatatctat
    23281 ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg actggggaat
    23341 tgatacttgg aattctgcta aagaaatggc atcaaatgca gttgagggtg tcaaagatgc
    23401 ttggtcagga acaaaggaat ggttttctaa cacctggaaa ggtattaagg atggggccac
    23461 aggtttattt gataaaacag tagagacttc aagaaatgca gtcgatagtg taaaaaatgc
    23521 atggtctaat atgaagaaat ggttttctga tacttggcaa agtataaaag attcagccag
    23581 agaaaaatgg aatggaatca aaggttctat tatgtcagtt gctggtccat tgattacagg
    23641 tataaagaat gcatttttac atgttacttt ttatctagag accttatgga ataatttagt
    23701 tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt
    23761 tattacctcc atgatttctg gtggatggga agaaacaaaa aataatatga ttgctgtttg
    23821 gaataatatt aaagaaagtg ccataaatat ttgggaatct ataaagaata tatttgtaag
    23881 ttattttaca aatatttatt ttgctgcact caatatttgg acagggttca agcttacatt
    23941 gataaatatt tggaatgaag tggtaaatca agctaaatcg atttggttaa acataaagta
    24001 tttttttatt aatctttgga ttgatataaa atattttgca attcagaaat ggattgaatt
    24061 gaagttttct attattcaaa cttggatcga tttaaaatat aatgccatta ctacttggaa
    24121 taatattaaa cagtttttca aagatacttg gaaaaatatt aaagatacgg catacaatac
    24181 gtggatttct ataaaaaata ccatgattaa tacatggaat aacattaagg actctttctg
    24241 gaatattgtt actggaattg ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg
    24301 tgtttcaaat gctattaatc gggtaaaaga aatctttaat tcgttaaggg aagtcaattt
    24361 atttgaaatt ggcaagaaca tcattgatgg acttataaat ggtgtaaaag aaaagtggaa
    24421 tgctttgaaa gatactatca aaggtattgc aggtaatatc aaaaattgga ttaaaggagc
    24481 tttagatatt cattctccat caagatggat gcgtgatatg gttggtaaaa atattgttca
    24541 aggtattatc gttggtattg ataaagagca aagtaagtta gatcaaacca tgacaaattt
    24601 agtaaaaaca ccgtctgttc aacctgtagt aaatggaata agtcacctac caattacaca
    24661 aacaaagcaa aatatatctg ctagtgaaac aaaagaaatt catttacatt taaatgttta
    24721 tggagattta cctgattcaa tggttcggaa gattaccaca aaaattaaaa cagaattgac
    24781 aagacaaatg aaacgagatg ctggtgcagt aggaggaaca ttatatgcaa cttaagagag
    24841 gacaattttt tattaatcaa cattattctt ctgaatttaa tgtgtatatt caaaacaggc
    24901 ctgcttctgt ttcagctagt cgagtaattg aattgagaga gcgagaagga aatgattcta
    24961 tcattattga taaaggctat tacaaaaatg tgactagaaa gattgagtgt tactataaag
    25021 caccttcaat tgatatggtg caggagtggg aagatcgaat cactgaatgg ttagatatgt
    25081 cgtcttatag tgattttatt ctttactatg atgaacaata tatttatcaa gctgtagtaa
    25141 tagaaggacc tgaatttaaa ggaacaagaa aaacaggaaa tattgtccct tttgaattta
    25201 cagtgagtat tcgaccattt aaggaaaatt acaatggtag atttactatt cgacaaacag
    25261 aaacttttga aatttataac ccagagaagt attcttcaaa accgcttatt aaattgagtg
    25321 gttctggaga tgcttctttt tatattaata aggataaata tgatttgaaa tcgttggaca
    25381 gagaattatt tatagattct aaattagaag aggcttatcg aaaattagat ggtaatttag
    25441 aacatcagga tcaggtcacc ttatttttag attttccatt tttatatcct ggaaaaaatg
    25501 aaattaaatg gacgaaaaat attcattcat ttgaaataat gcctaggtgg tggagaaaag
    25561 tatgaaacca agaatatata gtcctactga aacagatttc tcaacgaatg gcttaggaat
    25621 tttaaaggat tgtacaagat gtggaatata tgaagtagct aatggaaaat atgaattaga
    25681 gttggattat cctttaggaa ctagatttga tgaatatttt gaaaatgact atcaaataaa
    25741 agcaaagcca aacgatcaag aagagtatca tatctttttt attgatgata aagatataga
    25801 tactttttta aatacagtaa ctatttatgc tcagagtcgt acaaatcgac ttggaagacg
    25861 ggtagtcact cttgtggaag tggactctaa aactggtcaa gaagcgatat caattattga
    25921 aactaaaatg gataaaaaat ctgacatacg actttattct gatattacgg ctgtttctag
    25981 cacaatcttt gaagcgagaa atgttttaaa ttgtattgct ggtgaacaag gatcattgct
    26041 tcaatattgg ggcggagaaa ttaaacgtga accatttaag ctttctttgt taaagcgaag
    26101 aggtcgcaat aatgttggaa cgattcggta tggaaaagat atgtctggtt tgaaggtcaa
    26161 attagattgg acaggtataa aaacaagaat tattccatat gctgatcctc aaagtgaagt
    26221 aggtacgact aatcgaattt atggttcgcc agtagatagt gcctatatta ataactatcc
    26281 tgatgtctat acagaacatg ttcagtttac agaagaacaa ggagtaaaag acattaagag
    26341 cttaaataaa atagctaaaa attactttaa aacgatcaat cctggctgtg ataagcctaa
    26401 agtttctatt acggttgaat ttgataagtt gactgatagt gaagaagcga aagaatttgc
    26461 gaagattaga aattatggtc tgtttgatac atttaaaata tatcataaaa aatataatat
    26521 ttatcttgaa tcaaaggtta gtggactaca atacgattct ttatctgaaa aaactttgaa
    26581 attagaagca ggagacattc aagttgcttt ttatcagcaa caagctgtaa ctattcagga
    26641 taaattaaaa gattatgcaa cgaataatta tatgagtgat tttaatgatt atgtttcttc
    26701 aatgatt
//
LOCUS       DANKAY010000031        26585 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000031 DANKAY010000000
VERSION     DANKAY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(23..1723)
                     /locus_tag="PWY98_001288"
     CDS             complement(23..1723)
                     /locus_tag="PWY98_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037049.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2230..3390
                     /locus_tag="PWY98_001289"
     CDS             2230..3390
                     /locus_tag="PWY98_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2037050.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3406..3849
                     /locus_tag="PWY98_001290"
     CDS             3406..3849
                     /locus_tag="PWY98_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037051.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4332..4862)
                     /locus_tag="PWY98_001291"
     CDS             complement(4332..4862)
                     /locus_tag="PWY98_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2037052.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4967..5518
                     /locus_tag="PWY98_001292"
     CDS             4967..5518
                     /locus_tag="PWY98_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2037053.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5671..6144)
                     /locus_tag="PWY98_001293"
     CDS             complement(5671..6144)
                     /locus_tag="PWY98_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2037054.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6282..7754
                     /locus_tag="PWY98_001294"
     CDS             6282..7754
                     /locus_tag="PWY98_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2037055.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7751..8461
                     /locus_tag="PWY98_001295"
     CDS             7751..8461
                     /locus_tag="PWY98_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2037056.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8813..9097
                     /locus_tag="PWY98_001296"
     CDS             8813..9097
                     /locus_tag="PWY98_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2037057.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9378..9797)
                     /locus_tag="PWY98_001297"
     CDS             complement(9378..9797)
                     /locus_tag="PWY98_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2037058.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(9799..10383)
                     /locus_tag="PWY98_001298"
     CDS             complement(9799..10383)
                     /locus_tag="PWY98_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2037059.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10430..11905)
                     /locus_tag="PWY98_001299"
     CDS             complement(10430..11905)
                     /locus_tag="PWY98_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2037060.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12086..12397
                     /locus_tag="PWY98_001300"
     CDS             12086..12397
                     /locus_tag="PWY98_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037061.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12512..14848)
                     /locus_tag="PWY98_001301"
     CDS             complement(12512..14848)
                     /locus_tag="PWY98_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2037062.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14990..15658
                     /locus_tag="PWY98_001302"
     CDS             14990..15658
                     /locus_tag="PWY98_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037063.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15661..16704
                     /locus_tag="PWY98_001303"
     CDS             15661..16704
                     /locus_tag="PWY98_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2037064.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16941..17822)
                     /locus_tag="PWY98_001304"
     CDS             complement(16941..17822)
                     /locus_tag="PWY98_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2037065.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17981..18709
                     /locus_tag="PWY98_001305"
     CDS             17981..18709
                     /locus_tag="PWY98_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2037066.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18865..19182
                     /locus_tag="PWY98_001306"
     CDS             18865..19182
                     /locus_tag="PWY98_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2037067.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19175..19783
                     /locus_tag="PWY98_001307"
     CDS             19175..19783
                     /locus_tag="PWY98_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2037068.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19838..20629
                     /locus_tag="PWY98_001308"
     CDS             19838..20629
                     /locus_tag="PWY98_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2037069.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20767..21255
                     /locus_tag="PWY98_001309"
     CDS             20767..21255
                     /locus_tag="PWY98_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2037070.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21306..22178
                     /locus_tag="PWY98_001310"
     CDS             21306..22178
                     /locus_tag="PWY98_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2037071.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22354..22716
                     /locus_tag="PWY98_001311"
     CDS             22354..22716
                     /locus_tag="PWY98_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2037072.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22725..23477
                     /locus_tag="PWY98_001312"
     CDS             22725..23477
                     /locus_tag="PWY98_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037073.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23491..25293
                     /locus_tag="PWY98_001313"
     CDS             23491..25293
                     /locus_tag="PWY98_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037074.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25570..>26585
                     /locus_tag="PWY98_001314"
     CDS             25570..>26585
                     /locus_tag="PWY98_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2037075.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVT"
BASE COUNT         8459 a         4637 c         5388 g         8101 t
ORIGIN      
        1 cggatgtatt tatccctttt gcctaagcac gtaaaagctt gtatctatga taaataaata
       61 aacatagcaa ggtaaaccag ccaatcaaag taagagaaag aatgaaatag aaatagattg
      121 gcgtttgata atgaagcgtg agctcgttga ttcctttttg ctgcttcacg attggggtcc
      181 cgatatctgt tacttcataa tcagtcaatg tttttccatt ttgctgtagc acagtccgat
      241 cgtagacgat aacagggata tgaacagttt tgtccccagt gtttttccac ttgattgtca
      301 aaagaccgcc tggatgtgtt tttataaaag ttgtttcgtt ttctagtatt tcttcgttat
      361 accggtcgta tttattgtct tttgtctcct tatagattgg cagatagtca ggtgtggttt
      421 tttggaaaac gaacaagctg gtagacatat ctttgctgaa gaatgttttc cttgcttctt
      481 ccggcgtatc gaatagataa gtatgtcgac tgacaaatgt ttcattttcc cacttttcca
      541 gatgctggta taagttctgg atcgtctgtg ctgaaaaaat aagaatgcca aatatactta
      601 aaagacggaa ccacttttgt tcagaccact ggttcagtga aagggcaagg taaagtagaa
      661 gcaatagcgt aaatggcacg aaaaatcgaa atggaaactg gatcaaacta ataaaaggca
      721 catttttttc gatcaatgtc gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa
      781 gacttgtgta caacacgatt ttttggaata aggtgttctt ttgttgaaag aaaataaaaa
      841 gcaaaaaacc tgataatatc aatataggaa aggccttagg gtaatggagc caataactgc
      901 catccagatc tatcgccttt tgactcattt ctctgttaac aaagggagga agaatcttat
      961 tcccactgta aacaatagct aatcctgccc aaatatttgc tgtcagaaca aaataaagca
     1021 agaccgactt aatcagttga agcagtgctt ttcctttttc agtctggtta aaaaagtaat
     1081 aaagataaaa agggatatac atcaaagcta acatgatcga agacagtaaa tggatctgtg
     1141 tcataagtcc cactgagaca gcgactttga taacagggaa ctttttatga aggacgaaat
     1201 cagcaatagg gatcatacat aaaggataaa aacaagtccc ccagctagtg aatccttggt
     1261 tgatagacca gtattgaata gaatacgtac tcatgaataa aacggctact ggtacactta
     1321 cccaatttcg aacattgaca tatctcagta aaatcagcat agaaaatcct gaaataagat
     1381 aaagaatgaa atttgaaaga atctgataat gaaaccagct acctgcaagc aacacgatca
     1441 atccttgtaa ataagcgaaa aatgggccgt aaacggcatt cacgatcctg ccactttgct
     1501 ggaatccata aagggaagga aaataatgga aatccccata tttaatttgt tgcgcggcat
     1561 catagaaccg attgtaatgg aatggggcat cagcacccaa aattagttta tgtgtgaata
     1621 aatgaggcat gagaaatgcc aaagcaatga aaaaaattgc taaaaatggg ccatattttt
     1681 tcaattgtcg aactcctttg caaactttag ataaattcat catatatcct atgcgagagg
     1741 taggcaagaa attagaaaat tcatgaaaat atttatgaat tttacaagaa aaacataagt
     1801 gaaagttcta aggagagagg atgcgacata agttttgttg catctccttg ttttgaaaca
     1861 gaatttttct taatactcga aacaaagtag ctttttcatg acctctcggt aaacggtgtt
     1921 caatcagctg cttctcggta ataagctcac tagcctagaa aatatgagaa gctattttct
     1981 gactagcgct cttattactc aaagcataac gcagttttca caacctcttc cttaaacggt
     2041 gttcaaaagc ttgcatctgc ggtaataagc ccaacccaac aaaaaatatg agtagctatt
     2101 tttttgggct gtacttatta cttgatgcag gacagctttt tcacaacctc atcggctttc
     2161 attttctatt tttttcctga acccatataa taaaagaaag aacaaaaaaa cttgcaaggg
     2221 aggacacatg tgaagcagag taagcaaaaa cagtctcgtc gacttttgtt tcgtttgttt
     2281 ttattaatat gtatcgtcgt agggttactg atcgctttat atccatttta tgtggattct
     2341 ttaaatagtt ttattgatca aaaacgtatc gaacaagtac aaaaacggac cgcagctgaa
     2401 attgaagtgc agcgaaagaa aatggaggag aaaaaccaac gattaactga ccaaggattc
     2461 aatcctggta ctgatccttt caatgaaaag aacagaaatg aaagtacaac atccagccaa
     2521 ttagaggaat ggctgatcgg aagtatcaat atacctaaga tccaattaaa tatttcactt
     2581 tatgatcggc tcaatggtag gatccttgaa aatggagcag gcgtcttgca aggaacctct
     2641 ttcccgcttg gtggaaattc gacgcattca gtgatctctg ctcacagcgg tttacctaat
     2701 cgacggttat ttaccgaatt agatcgttta gaaaacggag acacgtttat tttgactgtt
     2761 ttgggggaga aattggctta tcaagttaaa aatatacaag tggtactgcc agatgacacg
     2821 tctgttctaa cgatttcaga aggagaagac ttagttacct tgcttacatg tacgccgtat
     2881 atgatcaaca cgcatcgttt gctagtgaca ggacatcgga ttccttataa cgaatcggta
     2941 aaaaaagaag aaggaaaagg aaatcaggaa agaaccatgc ggcaatggtt tattcttgca
     3001 ggaacgataa tagctgtagt catactgtta gtgtttatcg gacgactaat ctatcagtat
     3061 agattaagta aaaaagtgct tgatttttca tttattatta gtgattcgtc agggaaaccg
     3121 gtaaatggta gttcattcat tttgaaacat aagaaaaaaa cacttactag aaatggtgtg
     3181 ccgttttcag tacaaagcga ccatcatggg aaagtcaagc tggatcaatt gcctggtgga
     3241 acttatcgaa tcgtttcagc tgatgatcct aaagttgctg tttcttttgg tattagaaaa
     3301 gtaaaacaag agaagatata cttttttgag gggcgaaaac ttgtaaaaga gcttcaaaaa
     3361 aatggttttt attttaaaat caatgaataa gtaaggagag aaaaaatgat ccgcacaaca
     3421 acaaaattgt ctgccaaaga actggaatct gttctatcga tttggctgca tgctaacttg
     3481 gattctcatg aattcattcc atcaacttat tgggagaatc aagtagaatc tgttaggaaa
     3541 gctttaccag atgcagtgat ttacttagca gaaatagatc aagaaattat tggttttgct
     3601 ggggtgattg atcagtttat cgcaggaatc tttgtgaaaa gggaataccg gcaacaaaga
     3661 atcggtacgc aactaatgaa aaaaataaaa gaaaccaata aaacactgac tttgacagtc
     3721 tatcaaaaaa atcaatcagc tattcatttc tatctttcac aaggttttac aattcaaaca
     3781 cagcaaaaag atgaagcagt aaatgaggag gaacttgtga tgaaatggaa tcaaaacgct
     3841 gatttttgat gaacagaata acgaaataat gctcccagcc taattttttg ctgggagcat
     3901 tatttcattt tgaagtcact aatttaactg tgtgaatatg cagttgttac aatctttgat
     3961 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     4021 ctatttttcc atttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttga
     4081 taaacggtgt tcaaacggct gcttctcggt aataagccca ctagcctaga aaatatgaaa
     4141 agctattttc taactagcgt tcttattact caaagcataa cgctgatttc acaacctctt
     4201 gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaacatgag
     4261 gaactatttt tccgtttctt tctctaatac tcaggtcaga acgctgattt cacaacctct
     4321 ctttcacaac cttattttac tcgtttataa ttttcatcga agtattttcc gctacggata
     4381 tcatctggca ttccatcgta tggttcttcg ctaataacat cttcattgtc tatacctgca
     4441 tcacccatat aagtgatcgt agcaaattca ggaacaagta attttggata agtttcatat
     4501 ttttcacgag ctgcttccat ttctgcgata tgttcgtttt ggaaagtaac agtgatttct
     4561 ggatgttctt caacccattt agggaaaaag atcattccgt gaagtttctt ttcatttggc
     4621 ataaagtcca atgccacgtc tgtccctgtt ggctctgtcc aagtgatttt gaatacgccg
     4681 tccgtcaatt taacgatatc agcttcttgg tcttttaccc aacggccttt gaccattccg
     4741 ccatggatac ggtaatcaac agtatggtca tttttggcat accattcata ttcccaacca
     4801 ttgtcatacg tatagataaa gtgagttcca ataaaatctt ccaattcttt aaattctttc
     4861 atgtttaagc atctccttat aaattaaaac atgtaataaa ctacatgttt tcgattactc
     4921 atttatgata gaatggattt aaagatttgt caaggaggga aaactgatgg caagaaaaaa
     4981 tacacttcga tatattttgt tagggttatt aagtaagaag aagatgacag gatatgaatt
     5041 gaatcaatcg ttcaaaaatg aaataggaga attttggcaa gcaaaacata gtcagattta
     5101 tcctgaactt gcaaaaatgg aagagcaagg aatcattcag catcaagtag agatcacagg
     5161 tgaaaaattg gaaaagaaag tttatgaatt aacagaagaa gggaaggaac aattggcaga
     5221 gtggattcat acacccacca atgaactacc ggttaatcga gacgaatttg ttctgaaatt
     5281 gtatttcgtc aaagacgtga atgatccggc attagtagaa attattcagc agcagcgtga
     5341 ccttcatgaa gaaaaacgac ttcatctatt agagcgaaaa aaaacgatct ttcctactga
     5401 aaaagaaaaa catgaaaatt acggacacta cctgatttta gcgcatgcga tcaatcggga
     5461 aacagaatat gcaaattggc taacagatgt attagaagaa agtaaccgaa aaaaataaaa
     5521 aaatatccga acagtgatgg agaattcctt tctatatgaa agaaacgcag atcaccgttc
     5581 ggatattctt atgttcaaac aggccttttt tcaagtattt atgtagcatc tacttattaa
     5641 aaagcttggt acttaggaga ttagtggcgt ttagagttct tttaacaact gttcgtagtc
     5701 agctttttca tcgacaggaa ggagcttgta agttaattcg tctgtctcgt tgtcatgaat
     5761 gatgtacatc ttagcattct cttctttgaa gttaccgatt tgtaaaatcg tatagcgttc
     5821 gtcgatcaat tcgatatctt tgattgcttt tgaaaaaact ttacggacat ggtgcatttt
     5881 cttttggcgg atttctgcct tttctgcagc ttttgacaaa gtaaatccgt catctaaata
     5941 ttttttgatc agttctactt ttatgactgt aggtaaacga tattttctag gacaattcgc
     6001 atcagcttcg atcgaacgaa tgaatccctt ttgttcccaa taccttaatt gacgtggaga
     6061 cacacctacg atatcactta attcactgat gccaacaacc aattgatcat tttccaacaa
     6121 acgtttaagg gaagccccaa ccatttttgt cactcctttt gtcctcttgc ttcccttaat
     6181 tatatccgta ttaacttatg tgtcaagtag gttgacaaat agcgaattta ggactaaagt
     6241 attctagtag tttcagaaaa aattcagaaa gaaggagaaa tatgagtaac aaccaacccg
     6301 tagatattta cggaaaacca tataatcgga cacttttagt tgttgtctta ttaattggta
     6361 cattttgtac cgttttgaat cagaccttat tgacgacagc ttttcctaca ttgatgaaag
     6421 ctttcgatat ttcagcgtct gatgtgcaat ggttaacgac tggtttcttg ctagtcaacg
     6481 ggatcatgat cccaataaca gcatggctga tcaataaatt cagttcaaga aatttatatc
     6541 tttctgccat gacgatcttt ttgatcggaa cgattacttg ctttacagca cctaatttta
     6601 gtacattact aatcggtcgt ttgatccaag cttgcggtgt gggagtatcg atgccattat
     6661 tgcaaaatat catgctatct attttcccgc cagaaaaaag aggatcagca atggggatgt
     6721 cagggatcgt tattggactt gctcctgctt taggccctac attatctggt tatatcattg
     6781 ataactacca ttggcgtgac ttgtttggta tggtactgcc aatcgttgtt cttgtcttgg
     6841 ttttagcatt cttcctaatg aaaagtgtta ttccattgtc aaatcctagc attgatattt
     6901 tatccgctat tttatcaacg attggattcg gtagtttact ttacggattt tctagtgtag
     6961 gagatgacgg ctggggaagc ttgaaagtaa ttggtttctt agttgttggt gtgattttca
     7021 tcgccttgtt ctcatggcgt caattacatt tggaacatcc attcttggaa cttcgtgtat
     7081 tcaaatcatc tacattcacg attgcagctg ttttagctgg tgtaacaaat atggcgatgg
     7141 taggggcaga aatggtcgta ccattgtata ttcaaaatat tcgtggagaa tcagcattcc
     7201 attctggctt gatgttgttg ccgggtgcat tgatcatggg ggctatgatg cctgtgacgg
     7261 gtgcgatttt cgataaatac ggcgcgaaac gtttagccat cacagggatg ttcattttga
     7321 cagcggcaac tgcaccattt gctttcttga caaaaacgac tccagtttta tatatcgtga
     7381 tcctttatgc gatccgtatg tttgggatct ccatggtcat gatgccggta acgacttctg
     7441 gtatgaatgc attgccaaat aacttgatca gtcatgggac agcggtaaat aatacattcc
     7501 gtcaaatcgc aagctcgatt ggtacagcta ttttgatcag tgtgttgacg aatgtgacaa
     7561 aagcaaatat cccaagcaaa tctgtattgg ataatacgcc attagcttat aaagatcagg
     7621 caatcaatgc cacactgtca ggttaccatg ccgcgtttat cgtagcgatc atctttggga
     7681 ttcttggctt tattattgcc tttttattga agaaaaaaga acctgttcta atgaaagcgg
     7741 gggataatgc atgattattg gagttttgat tgtcagcttg cttgcttttg ctttaacgaa
     7801 cgtgttcgct aaaaaaacat ggcaaacctt tttatctttg attttcggac taatcttcgt
     7861 cgcttcacta agtttgatcg tcgctaattt gtccaatcac tttggaatgg aaaaagtaac
     7921 ggaaacaaaa acagaaaaaa ttgtttccag tgctgattcc caaggagcag atatgctttt
     7981 gtataaagct ttagggaatg gaaaagaaaa agtgtatctt taccgaacaa atgaaaaaca
     8041 agaaaaacca aaagcaactg gtacggataa cgaaacaaat aaagtagaaa agacagacgg
     8101 cgatgctgaa aaagtgacga aaactactta ttgggaatac aaaaacgaca tgtataaatt
     8161 ctggttcaat attgctgata acaatcacga atatgacagc cgagtgaata cattcaaaat
     8221 cccagagaca tgggtagaat taagtacgga ccaagcagcc aagttagctg aattagtgaa
     8281 gaaacagcaa agtacgatgg aaagtgaagc aaaagcttat gttcaagacg gtatggtaaa
     8341 agcaatgact gaaaatccaa aaatgagcaa agcagagcaa gaacaacgta cgaaagcctt
     8401 agctgctgaa ttccaacaac aagcattcgc aaaattagtg aaagaagcaa aaggcgaata
     8461 atattttgaa caccatgtag gaggttgtga aaaagctgct ctgcatcaag cagtaagaac
     8521 aatcagataa aaatagcact ccatattttt catctgattg ggcttagtgc cgtagatgca
     8581 agcttttgaa caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
     8641 ggagcaattt caaaaaacag cttctcatgt ttgttgacta ttgtgacttg tgaccgagga
     8701 gttggcagga gaacaccgtt tattcgaaaa aaggaactgc gacaagactt ttgtcacagt
     8761 tccttctttt ttttgctatg ctatactaga tgtaaaaaat aggagaaata aaatgaggga
     8821 aatttattct tttgaatcaa aaatctatgc ttccgaagtc ggcaagggcg gggcatatat
     8881 tatttttccg tatgatataa gaaaagaatt cggtgctgga agagttaaag tccaagcaac
     8941 ttttgacaat gtgccgtatg aaggcagtat cgtgaatatg ggcgtgaaaa atccagatgg
     9001 ttcagtctgc tacatccttg gactacgaaa agatattcgt aaaaacatcg gaaaagaaat
     9061 cggtgatatt gtagcagtta cagtgaagca aaaataaaag aggttgtgac aaaagacgtg
     9121 tcacaacctc ttgataaacg gtgttcaaaa gccagtttct cggtagtaag tcaaatttat
     9181 gcaaaaatgt ggaaacccat gtttacaaaa tttttcttac tactcgaaac gaagcggctt
     9241 tatcacaacc tcttgataaa cggcgttcaa tcagctgtcc ctcggtatta gctcaaaaac
     9301 ggcaaaatat gagaagctat ttttccattt ttttctctca tactcaggtc ataacgctga
     9361 tttcacaacc tcttctttca taatggtttt tcaccatctt tataataaac tggttctttg
     9421 atatggaaat ctattttttc caaccaatct aaaatcttgt aacctaactc ttctgacatc
     9481 ggaaaagagg cgttgatttg tgcattggag atatggtcaa catggatacc ggaagtgatc
     9541 gtacaacttc ccggaacggc agttttgatg atcacagcta ttttttcggc taagacatcg
     9601 tctttatgga accgaccatc gtggctaggg aaacgtacag tttgcatctc tgtatcacga
     9661 gaaaatgtag tgactgttcc gatatgtggt gtatctccgc ctgttaaagt gatcaataag
     9721 tctttaccga ttactttaac agcagcttgg atcgtgaaac ctagtttggt tacttcaact
     9781 atcgtttctt tttccatatt aatcaccctg ccaacgtttt gaatgctcat ttacaatttc
     9841 aaattgatcc aaaagtttca ttgtatggtg attaacaaga tcttggatcg tttgaggctg
     9901 attataaaaa gcaggaattg gtggtatgat ttgcacacct agtcgtgcta atttagtcaa
     9961 gttttccaag tgaatggcat ttaatggcgt ttcacgagga acaatgacta actttcgttg
    10021 ctccttcatt gttacatcag cagcacgtgc aatcaggttg tcgcctaaac caacagagat
    10081 acctgcgact gttttcatac ttgccggtac gatgatcatt ccatcggtca aaaatgatcc
    10141 gctggcgaca gcagcaccta aatctttgtt tgaataatgg taatcaaaca ggctttcgaa
    10201 ttcagttagt gataagtcgg attccagctt taagttctgt tttgcccatg gactgatgat
    10261 tccgtgtgtt tctatcatcg gatattcttt tagtttcttc actaggttga tggcataaat
    10321 ggtgcctgaa gcgccactta caccaatcac gatttttttc ttccgttgtg ccaattgttc
    10381 cattttccgc actccttttt atgatgtcaa aacagttgca gatcattcat tatttcaaat
    10441 atttttccca atcttctact tcttggaatt ttgcacgtac aaaatgttct ttcatatcga
    10501 aaggaactgt accgtcaaag atagtttttg cacttatccc acggaaacga atagagccag
    10561 gattatattc tggtaattct gatgggtcca atggatggtt cctcattcct gggatcacga
    10621 tgatatcttt atctccttgg aaacgagtgt tgaaagtcca aactacatcg ttcatgtcga
    10681 aaatatcaac atcttcatcg actaaaatga ctgtcttcaa ttctttgaag gctgataaag
    10741 caagaatagc tgcttgacgc tgaatacctt catccgcttc gttttcttta cggatttgca
    10801 tgatggtcat caatttacca ccacctgcag gaggattgta gacatttact acttttcctg
    10861 gaatagcttt atcgaccaag gcaagaatac ttgcttctgt aggaataccg gccatagata
    10921 cgtgttcttc actgggacca atcgttgttt gcatgattgg attgtctttt cggtgagtaa
    10981 ctgctttgac tttcactact tgtacagctg gattagcatc cccagtatag ccaggaaatt
    11041 ctggcattgc ttttcctgag ttcgtgttga tatcttcttg cattgtttca tttggcatga
    11101 tttctgcttc aatgatgaat tcagcacgag caatacccac ttcatcgact gctataccat
    11161 caacgatttg aataggttcg ttacgcaaag ctcctgcaca ccatagttca ttgtagccaa
    11221 gtggagtggt aggtggttcg aaagtcgtgc caatggcaat tgctggatct aaaccaatat
    11281 tgatagtgat aggcattggt ttattcaagg cttcgaattg tttttggaag tgaccaatgt
    11341 ggcgtccgcc aggcatgatg tacataccga tcgtatcttt atcttctaga accatacgat
    11401 ggatcgttac atcgctcatt gttttttctg gatctgatcc taagactaca cccattgtga
    11461 tgtatggacc agcatcttca ggagtatttg taggtgcagg caaaatgttg cgaatatcga
    11521 agtctgcatc ggtagctaga tgaacgactt cttgtgctgg agcgtctgct ttgtctactt
    11581 tcacaggttt gattggattt tctacagcgt ctttcaagaa acgaccaagt gttcgataat
    11641 catggtgcaa aatttcacct acacgtttac gactagccat tgttccgatg ttcacacgtg
    11701 tgccggggaa acctttgata ttgttgaaag tcatagctgg tccttcttga gtcggacgtt
    11761 ttactgtacc gccagcaccg atataacggt aaacaccaga taattctgct tcaggatcga
    11821 tttcaatatc tgtttcgtga tattgacctg gaagtgtttt tagttcttcg atgacttttc
    11881 ttaaatcata cggttgatct gtcatgagag attcctcctt agttgtcttt acctatctat
    11941 agtaaaaggt gaaaaagagg aaaacaattc aacttgttcg gtaaagtatt ctacaatgga
    12001 atggatgcgt ttttttgata atagaataaa ctatgatata cttatcacta aaagaaagcg
    12061 catacttagt gttggaggaa ggaaagtgaa aaaaatggag gagtcatttc ctaaagcagt
    12121 aaaagtggaa aatatagcga atattttgaa agttacattt gaaaacggag aagtaaaata
    12181 cgtgaaatct cattggacag aagagataac cgatgccttg caatttggaa aaaaaggaag
    12241 aggaaaaaga aaaaatcttc tcgcactttc tcgtaatatg tggattggga ctgaagtaac
    12301 gattgaagca gatggaacag tattcataaa tggaaaagac aggtatacgc cagaagagtt
    12361 gtggtacaaa ggaaagaaaa gtataccaga attataaaaa ataattaaaa aaaacagcgc
    12421 gaaacaagga acatattact tcctcgtttc ttgctgtttt ctactatttc agcgctctta
    12481 tgactcaaat ctaaatgtgg ttgtcacaac cttattctgc acgcaaagcg atagcggcgt
    12541 cttttttcgc tgccattcgt gcaggaagat gaccgccaag catcgtcaat atagtagaga
    12601 cgatcactag aataatcgca tggacaggat ttagttgagc aacatttttt agatccgtca
    12661 tgttgtataa gatgctattg atagggaacg tagctagcca tgcaataaac acacctaata
    12721 tcccagaaga aatacctaaa atacacgttt ctgcgtcaaa tacgcgagtg atgtcttttt
    12781 tacgtgcacc taaagctttt agtacaccga tttcttttgt ccgttcaatc acagatgtat
    12841 aagtaatgat actgatcatg atcatacttg ttactaaaga gatccctgca aaagcaataa
    12901 ggacataagt gatagcatcc atcaatccgc cagtcagttc agtcatcgtt ccagctaaat
    12961 cagtataaat gattttatct tcatctgatt tgcctttatt gtaatcatct agataatcca
    13021 agatcttttc tttgtcttca aaattgttcg gataaatcat gatacttgaa ggcaagctgt
    13081 ctccacctaa ataactaagc aatgtttgtt tggcattttc gtcaactttt tcagtagtta
    13141 agacatttac accactgtct ctttgagctt tgacgatttc tgattctttg ttttcgttga
    13201 cgatttgagt cgttaattga tcgctataag cgattcctgg agataagaga ttcattgtag
    13261 aagaagattt cacgcgtagg atccccgaga tcttcaattc atcagaagca ttctgataca
    13321 tcgcatcata atctgtatta gggataaaat tcccagttgg aagttttgta tagtaagtat
    13381 tgttgtttac taatttgaat gttgtaccaa cgatatcatc aaaatcaagt ttttcgtctt
    13441 ctttcacatc aaatccaaga ttcttcaatg cgttgatatt agtattatta ttgccgtcta
    13501 cgatcagcac gacatctgtt gctgaggctg gataacttcc ggcaagtaaa gaataattgt
    13561 cttttaaaaa gttttctttg ctagtgtcaa gctgtgtagg gaaacttgac actcccacac
    13621 ctgtcattgc tgacatcgta ctagataaag aaagagattc cgtatcagga ttttgattcg
    13681 agaagctgac aggctgtact ttgccattta catcacgtaa aagattgatt cctgtagtac
    13741 gggtaaatcc gatattatta cttaagttag gatcgatgtc tgttacgtaa tcgacgtatg
    13801 tttgatcaat cttattcgtg tgctgtgctt tatcttcttc gctgactttt gcagtaatcg
    13861 ttttgctgtc tggataatcc gctttgcttg cacccaatcc ggcatcgtca cgagtctggt
    13921 tagtcgttac cttagaaata gtaatcggaa atttcgccat cgtttcagct tgcgtgttat
    13981 cgatttgttt ttggaatcca gaagaaaggg acagaacgat cgcgattccg atgatcccaa
    14041 tactggaagc aaaggaggtc aagaaagtac gtcctttttt tgttcggata ttgttgaagg
    14101 aaagctttaa cgctgtccaa aaactcatct ttgttctgcg taagttaaat tgatcatctt
    14161 ttggtcgttc gatgtgagga tgggaatctg ttaagatctt accatcagaa aattcaatga
    14221 ttcgatctgc gtattgatgg gccagttcag gattatgtgt gaccatgatc actaattttt
    14281 ctttcgataa ctcttggatc aattccatga tctggatact tgtttctgta tctaacgctc
    14341 cggttggttc atcgcacaaa aggatatctg ggtcattggc tagtgctcga gcgatggcca
    14401 ctcgctgcat ttgtccacca gataactgat ttggcttttt atgcatatga tttgtcaagc
    14461 cgactcggtg caatgcatct tctgcccgtt ttcgtttttc gtctttagaa acaccactta
    14521 atgtcatccc aagttcaacg ttttcaataa tgcctagatg accaatcaag ttgtaacttt
    14581 gaaaaatgaa accgatagag ttgtttcggt aggcatccca gtcactgtcc ttaaagtctt
    14641 tagtagactt tccgttgatt accatatcac cggaatcata attatctagt ccaccaatca
    14701 cattcagcat agtcgtttta ccagaaccac taggacctaa aatcgctacg aattcttttt
    14761 gtctgaaagc gacagacaca ccatccaatg ctttggtggt ggtttcgccg actttataat
    14821 atttttttat atcttttaat tgaagcataa tcacccttct cccgtattga aatattcggt
    14881 atactaataa gtatatcaga acaatgatcg tagtatacga aataaatgtt aactgaagat
    14941 gaactacaag aaaaaatcta gaaatttttc aaaaaacaga gggagatcaa tgaatcgtat
    15001 tttagtagta gaagatgatc cgcaattaca gttgctttac cgttctgtgt tggaacgagt
    15061 gggcttttca gtcatcaccg cattgaatgg agaggatgcg ctgaaaaaac tagaagcgac
    15121 acgtatagaa ttaatcatta cagatatcat gatgccgaaa atggatggct atgacttgct
    15181 agactcatta cgtagcacca gatttgacac acctgttcta atgattacgg caaaggccga
    15241 ttttgaggat aagaaacgcg ggttcaaatt aggagcagat gattatatga caaaacccat
    15301 cgatgtcaat gagatgatcc tacgtgtgga agcattgctt cgacgttcaa agatcaatta
    15361 cagcagtagc ttgataatcg gacaaacaaa attagagcta gatgcttata cagtgttaca
    15421 agatcagcat tcgttgattc tgcctcaaaa agaatttctg cttctgtata aattactgtc
    15481 ttatcccaac aagatcttca ctcgccaaca gttgatggat gatatttggg gattagatac
    15541 gaatacagaa gaacgaacga tcgatgtaca tatcaaacgt ttaaggaccc gttttgaaaa
    15601 gacggaagat tttcaaatca tcacagtacg gggactaggc tataaggcgg ttatttaaag
    15661 atgaaaaaaa agctattttc acaattatgg atctattttg ctagtattgt cttcatttcg
    15721 atcttagtga cactgctgtg ttttctcggt tttattttct tattgtctcg acattctatc
    15781 tcgcctgctg aatcggctgg aaaaccaacg ttatacccgt tattcgtctt tgcggggttc
    15841 agtatggtcg tgggtacagg aatatcgatt tttgttgggc ggcgtatcct gcatccaatc
    15901 agtgcgctag ggacaaatat gagtctggta gcaacgggag atttttcgat tcgaatggat
    15961 gaacagcaga aagtagcaga agtacagcaa ctgtataaag atttcaacgt tatggtccaa
    16021 gaactcaata gtatcgagac actgaggaat gactttgtgt ccagtgtatc ccatgaattc
    16081 aaaacaccac tagcaacaat ccaaggatat gttcaattgt tacaagcccc aaatctctct
    16141 gacgaagaac gccaaatatt tttatatcgg attatcgaaa gtatcacgca gctttcacaa
    16201 ttaacagaga acatcttaaa attgaacaaa ctggaaaacc agcgtatcca actggaaaag
    16261 aaagagtatc gattagatga acaaatcaga gaagtaatcg ttttttttca gcctaaatgg
    16321 gagaaagaac agctagaatt ggatatcgaa cttgctgcag taaattacac aggcaatgaa
    16381 gagtttttgt accaagtgtg gctgaatatc atggataacg ccataaagta caatcaaatc
    16441 aatggacaga ttcatatcaa actatttgag acagccacag agattgtact ggaagtgacc
    16501 gattcaggtg taggaatgaa tgaagagacc agagatcgaa tgtttgaaaa attttatcaa
    16561 ggagatacaa gtcgtcaaat ttctgggaat gggctaggac tttcactggt taaaaaaatt
    16621 ctagaactgc atgatggacg tattgattat agcagtatcg aaggagtagg aacgacagca
    16681 atgatacggt taagtaaaca ataggaaaaa gaataaaacc cctaaaaatt ctattgaagg
    16741 tttttagggg tttttagtcg tttttttgtg attagggaaa tgttcttacg gttcttttgg
    16801 cataaaaatc gctgctttct tattgaacta cgagaaatgt tagaggtgtc attgaaacaa
    16861 ggacatgatg caagaaagag agcgaaagtc atgcgtcgct ctcttttttt gggtggatag
    16921 atggacagaa aatgtagggc ttagttttct tcttgattta aatactccca aaatgtatct
    16981 gaagccaaag aatgctcacc gactttacga gtaaatgcaa gataactctc tgttgttgga
    17041 gaaataggga gaatcacagt attttctttt aatagattct tgactgtttt ttccataaca
    17101 atagcaatcc ccatgtcatt tgcaatcata ttgatgatca agtcgattct tgctccttta
    17161 taagagattt ttggttcaaa tccagcttcg cgacacagat ctagtacagg ttgatacaag
    17221 ttcgtagtgg ggccaaggag caaaaagttc tcctttttca gttcgcttag atccaatttc
    17281 tttttactag ctaaaggatg atttttaggt agaactgcta caaaatcgtc tgtttcagta
    17341 ggcaaccact caatcgaact tggtggggcc tggaaacttc tagcaaagtg gatcgtgtca
    17401 ctatgttcat tggcgtcatt gtaagttaat tcgacacttt ccacttcttt caactggact
    17461 gtaaattcag gatggcgttt caaaaactcg gtgattttgg aaaaaccttt ataattgatc
    17521 attgtcggta tcgttaagat atttaatgaa agattgttgt ctttcgtata ggtttcaagc
    17581 acttcttgca tagcatggta ttgttctacg acatttttta catatgggag caataagctt
    17641 ccagcttcgg tcaattcgat ttttctatgg gcgcgttcga ataatttgac gtctaattct
    17701 gtttctaacg aaagaatctg tttagaaata tttccttggg tggtgaattg tcgatcagca
    17761 gttttggtga aattcattgt ttcggctaaa tcaagaaaaa cttcgaattt atggatatcc
    17821 atcatattcc tcctacttat tccaataaag aatactttaa cacaaaaaat gaattggtgt
    17881 aatcttctct gtctgttaca atctttcttg cagcgaaagc aataattaca gaaatttttc
    17941 tgattttttt cgcttcgtag aaaaagagag gagttttttg atgccaactg gagttttgat
    18001 caatgtaggt tccgttttgt ttggcggact aattggcgga ttagtaggaa ataaattaag
    18061 tgaacatttt aaagcacagc tgacgatggt ttttggtgtg tgttcgatgg ggatgggaat
    18121 ctattccatt gcgccgatga aaaatatgcc ggctgttatt tttgctttag tgataggaac
    18181 agcgattggt ttgattgttc atttaggtaa tgggataaat aagggggctg cgctgatgca
    18241 agtacctatt tctaaaatct tccctagtga aaagcttggt atgacccacg atgaatttat
    18301 cagcacatta gtgacggtca ttgttctatt ttgtgcaagt ggtacgggga tctatggaag
    18361 tttagattct gggatgacag gagacagtac gattttgatt tctaaatctg tccttgattt
    18421 tttcacagca gctatttttg cgtgtaattt agggtatgtc gtttctgtga tagcaattcc
    18481 ccaattcatt attttttata ttttgttctt attggcaaaa tttatttatc cattgactac
    18541 accagacatg atcttagatt ttaaagcttg cggcggattc ttgatggtcg ctaccggttt
    18601 tagaatgatc aacgtgaaaa tgttcccagt agctgacatg atacctgcaa tgatcgttat
    18661 catgccactg agctggatgt ggacaaattg gattatgcca ctgttataat aaaaatcaaa
    18721 gagaagccat tttttgctga ctaaaaagga tcaaatcttt tagtcagttt ttttgtattt
    18781 gacaaactga ttttgaaact atattatatg ttgtataata tagattgaaa taaaatatta
    18841 cacttgagaa tagaaggtga cgtaatgcac atccagattc caactgttct tcttgatggt
    18901 actgtgctag cagcattaag caaggaagat atgtatgggt atgccttaac aaaaatcgta
    18961 caagaaagtc tctctgtcag tgaatctaca ctatatccgg ttttgagaag attgaaaaaa
    19021 gaaggatatc ttgagaccta tgatgaacct tttgaaggca gaaaccgtcg atattactcg
    19081 ttgacaccaa tgggaagtcg tcatttagaa gaaattattg atgaatggat tgtttttcga
    19141 gattctttag accggttatt ggaggagagc acaaatgaat gaatatttga atgaagttgt
    19201 ggatcatctt caagaaattc ctgaagacga tcgttttgat ttgatccagt attatgagga
    19261 atatttttta gattctggaa aaacattaga tcagatcatt gatgaatatg gtacaccaaa
    19321 gcagtttgcc ttaaaattaa aaatcagtta cttttcagaa gaagaacaag cagaaaacga
    19381 gttagtgaaa aatccaaaaa gacaaattcg cctgatttgg ttaatcatta tcggtttatt
    19441 tgcttctcct gtcctcattc cactagcact ggggtttata ttggttattg ggggactatt
    19501 ggttgcttta ctagcgctca tttttagtat ctatgtttta tgtatcagta tcttaggtgt
    19561 tggcctattc atgatcatct caggaatcag tgtgtttgga caatcgattc tgagtggatt
    19621 attctttatc ggtgtcggtc tgttcgccac aggaagcgca gtatttattt cgccactgtt
    19681 gctgaaagga acaaaagggc ttttttatgt catgatgaac tttgttaaat ggatgggacg
    19741 acgttttatt acaaaaagag atctgcattc agcgggcgtt tgaaggagga cacataaatt
    19801 gagattgaaa cattttttga ttaccggtat tggtctgatg cttactggtg gcctcctagc
    19861 aggtgcaact tatacattag gcgctcaaaa gagtctgact tgggataatg gtcctcggtt
    19921 gatcgatatg gttactgaga caaaaaaaat caaggatgca aacaaaatcg tcatccatgc
    19981 agaaaatcaa acagtttcgg tcatacgcgg attggatttt gagataaaaa cgacttatga
    20041 taagacagaa aagccagaaa taaaggagaa aaatggcgtg ttgactgtga atgcagtcaa
    20101 aaaatcaaca ggtgcaatca ttagtttaga agacacaagt gcacaaatca ctattgttgt
    20161 ccctgcagat catgaattat ctgaattgac aatcgaaggg aataatagtg caattgatat
    20221 caacgaagta tcaatcaaaa aagtgaatac taagacacat gatggctggg tatcgttcaa
    20281 tgaatttaca ggaggaacaa taactgcgaa agatgaatta ggctcacttc gattagatga
    20341 ggccaatgtg gataacttga tcgtcacagg tgaaaacagc aacttccatc tagaagacag
    20401 tttgatcagc aaagaaggaa aagtcatcct aaacggtggc aatttatctt tgcatgagat
    20461 ggaaaacagt ggctatacta tccaaacgtc tggtgaatct tatattgaaa aagactacca
    20521 gccaataaca aatactttgt tagaacaagg acagaaaaag ctaaagatca ctgctaaaga
    20581 cacatcgatc aatattacga cggatcaaga ggacaatgaa acagattgat cctgtactca
    20641 actggatatt ttaagaaaaa acagcaaaga tccttccaat ctttgctgtt tttcatcgct
    20701 taacgcacat aactatagta aaataagaaa gaaaatatct aaaaagaaaa cagggtgaaa
    20761 gagtatatga aggtagaaga aggtcaaaga gaaaagttaa aaacagaaat ggaaagatta
    20821 catacttata tcactcaact aagccaaacc ttctatgatc cagacaaaga aaaagtgatg
    20881 gttaattacc cgaacaattc agaaggtcgc caattagagc aagtgtacca tgaagttttc
    20941 aaacatttat tgacggtgaa gaaagagctg gattattatt ctttaccgat tatcgataca
    21001 ggaatattga agtatgatgg gaagaaggaa cgatttatct ttaaatctgt tcgggaagat
    21061 ttgcctcttt cagcagggat ggatctagag gtcttggtag aagactattt tacagaagaa
    21121 aagcactggg ttcgaacaaa attggattat ttaccgcagg cagccggagg gacacaggca
    21181 agcggttggt acatcactga agacaaagag ttagaactag aaggtgtaat ggcgcgtatt
    21241 cgaaaaaaaa attaaaaaag taacattaga tatttattga aggataaagg agcgcaggaa
    21301 gtagcatgag agaaaaaaga gcattttata tcaatggtta tgcagggatt gtcggattgg
    21361 tcgtattagc tttagttgga ttgtttttct tctatttagg tatgtggcaa gcgaaagttt
    21421 gggcactttt tttaagtatt tttctttggt tgatcacgtt attgttgtta agttcggcaa
    21481 ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt tggacaatat ttaggtacga
    21541 ttcgagaaaa tggctttttc cttacgatac ctttggcaca aaaaatgact gtttcgttga
    21601 aagttcggaa tttcaatagt tctgttttaa aagtcaatga tttggatgga aaccccatcg
    21661 aaatctcagc agtagtagtc tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag
    21721 cgtattatca agattttgta gaaatccaaa gtgagacagc cattcggcat atcgctagcc
    21781 aatacccgta tgatacattc aatgagaatg atttgacgct tcgaggaaac acgacggcag
    21841 tgtctgacga gttgcaaaaa gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa
    21901 tcgaaacacg cttgaaccat ttagcctatg caacagaaat cgctagtgcc atgctccaac
    21961 gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa
    22021 tcactcaaat ggccttagag caaatcgaag atggacaaga aatcaatttc accgatgatc
    22081 ggaaagtcca attgatcaat aatttacttg tctcaatcat tacagacaaa ggaacgcaac
    22141 ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca tactttttgt aagataggaa
    22201 gaaggagttt aggacggatt attgtcttga actctttttt tagttgtttt aaagaaaatg
    22261 acaaaattga aagaagaatg atagttgatt cgaaggcaga attgttcgct tttttctata
    22321 atcagtatat aaagtaagag agaaggggaa aaagtgaaaa aacttctgat cggtctactt
    22381 gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag attacgtagt tatgggtgga
    22441 gaagaatact atgtacaaat tacgacagat ggtacaaaaa cagtttcaaa atcagactct
    22501 ggacaagaaa cagtcaacta tagatatatc ctgcctggct atacgaaaga gggtaaggaa
    22561 aaagaactgg acttcaaggg acaattgact cgaccgcttc gtaaaggagc atatttaaaa
    22621 atcacctgga acaaaaataa aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg
    22681 ccgaaaacag cactggaaaa actaattaaa gggtagggaa agaaatgaac aaaatagtcg
    22741 aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga gaaaaagacg caagcattaa
    22801 gcggcatcag cttcgatgta gaaaaaggcg aatttattgg gatcatgggc gcttctggtt
    22861 ctggtaagtc gacacttttg aatattttat ccacattaga taaaccaaca gatgggcata
    22921 tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa tcaactagca gattttcgtg
    22981 cgaatgagat tggctttatt ttccaagatt tcaatttatt agaaagcttg acagcacaag
    23041 aaaatatcgc tgttcctctt tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg
    23101 tccaaaaaat tgcagaacgt ttatccatct ctcatatttt ggaaagctat ccttctgaga
    23161 tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc attgatcaca caaccaacga
    23221 ttctattagg ggatgagcct acaggtgcgc tggattccaa aagtgcccgg gatcttttag
    23281 atacgatgga cgaattgaat acaaaagatc atgtatcgat tcttcttgtg acgcatgatc
    23341 cgttttctgc aagttattgc caacgtattt tattcatcaa ggatggcggt atccatcaag
    23401 aagtgaaacg tggagatcaa agccgtgatt ccttctatcg agaaatacta tctattcttg
    23461 gaaacttgga acagtagggg gagattgact atgctttgga aattatcact tactgggatc
    23521 aagagccgtt taagagacta cttggtgtta ttctcaggat tagttatggc atctgctatt
    23581 ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt
    23641 gcggctacag ttatcatttt ccgattcggt tctgttttac tagggatcat cacattcgtt
    23701 tatattttat atgcaaattc ctttttgatg acgatgcggc aaaaagacta tgcaatgttt
    23761 atgatgctgg gagcaaaagg cagaaaaatc gcacagatga ttttttctga aacattctta
    23821 gtagggatcg cagctacttt agtgggttca gctgtcggtg taggattgac aagtatcgtc
    23881 caccgattat tagtggatca gttagatctc aatatttcgc attttacacc ttttagtatt
    23941 cacgggttgt tgatcactgt tttgtttttt gctgttttgt ttttattagc tgctgttgtc
    24001 aatgcttttt cgattgtcaa aaaaccgatt ttagcattga ttcgtgcgga tcagacacca
    24061 acaagaatca agcagcataa attcttgttt ttccttgaag tagtactggg aattgctttt
    24121 ctaggctgcg gttactatat gatgaaacac gtggggacct atcaagtctt tggtataggg
    24181 gtagccttag ttacgatcgt attaggaact tatttcattt tccattctgt gatcattttc
    24241 tttttatcat tactaaagaa atcagaacag atttcattga aaaaattgaa caatttcaca
    24301 ttgtcacagt taaactttcg tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg
    24361 ctgtttgcct tggcactggg cgctttgacg gtaggattag gttttaaaaa tgaaattgca
    24421 gacttgacag aaaacaattc tggatatgat ctagtgatga ataatcctcg ggcagaagat
    24481 cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt cttatactca aaaagaagat
    24541 gcgggaacga tttactataa tgcaagtgaa tttgcggctc aaccattgat catgataaaa
    24601 catgaagatg gaacaccgcc gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat
    24661 gaactgactg tacaagagga acttcgaagc tatgaattgc cggaacagca agaaaaagaa
    24721 atcaaggtag tcagtcaatc agaatttgat caactgaact tgccacaatc cactttacag
    24781 ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa
    24841 aataaaatga acaatccatc tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg
    24901 tatgaagtgt ataatggtct attctctggt tttgaattta tgggattctt cctaggttta
    24961 gctttcttga ccatgcttgc tagctgtttg atgttcaaga tcttgtcagg aagcaagagt
    25021 gacattgctc gttatgaaat gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa
    25081 tcgatccgta gagaagtagg cgtgttattc ttagcaccag gaatcttagg tgtagtccat
    25141 gtcttattcg gattacaaat gtttggtcta ttcatgcaaa atccatacaa agatatttgg
    25201 gttccgttta cgatattctt cgtattgtat ttcatctact atgtactgac gacatggtta
    25261 tatactggaa tcgtattgaa aaagagattg tgaaaaagct gtcctgcatc aagtaataag
    25321 aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttgggcttat taccgcagat
    25381 gcaagctttt gaacaccgtt tgttcggaaa aagaggttgt gacaatattt ttgtcacaac
    25441 ctttttttca cggtctttta ttagaatata gtaaattttt tctcgttact cacggaataa
    25501 gctgttaaga aaacggtttc catgtatact ggaatcagtg caaatgaaag aaaagagggt
    25561 gcaaggaaca tggaaatcaa tgaacaaaat tttggcgaaa actatcactt gattacgatc
    25621 acgaatcaaa atgggacatc tttgtctgtc agtgatcttg gggcacgggt tgttcatttt
    25681 caaacaataa tcgataatga aaaacgagag ttgatcttag gttttgatga agcaaaagaa
    25741 tatctagaaa aagaccctta tatcggtgct tcaatcggac gtatggctgg acggattacg
    25801 aatggcacat tcaaattaga tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt
    25861 cattgtttac atggtggtgc acctagcttt gaagccaagc agtggcatta tacagtcatc
    25921 aatggtgaaa acgaagcatc ggtcattttc tatcagacta gcccagatat ggaaaacgga
    25981 tttcctggaa ctttagatgt agaagtacgg tatacgctga cgaacgatga tacatggcga
    26041 gtgacggtcc aagggatcag tgaccgcaaa acagtattca atcctacaaa tcatgtctat
    26101 ttcaatttaa ccggtgatcc agcacagtca atcgacgaac atatactttg gttgaatagc
    26161 cgattatttg ctacgctcaa taaagataca acgcctacag gagaagtagt ttcagtaagt
    26221 ggcacagcat ttgacttcca acgtcctaaa aaattaggtg aggtctttgc cagtgatttt
    26281 cgccaatcaa tgatagtggg cggcatcgat catccatttt tgttgaaaaa acaatctggt
    26341 ttactaaatg ctgcggaatt gacaagccca gatgaaaaag tatcgatcca agtaaaaaca
    26401 gatgcttcag gaatcgtgat ttttacagca aattttggag aagatggacc ggaaatgcgc
    26461 gggaataaat tagtaaatca tggcggtatc acatttgaga ctcaagaact gccaggagcg
    26521 gagcgacttg aaagttttgg agatgtcact ctggaaccta accaagtacg cgagtttgta
    26581 acgga
//
LOCUS       DANKAY010000032        25207 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000032 DANKAY010000000
VERSION     DANKAY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(164..388)
                     /locus_tag="PWY98_001315"
     CDS             complement(164..388)
                     /locus_tag="PWY98_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2037076.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="PWY98_001316"
     CDS             complement(586..2166)
                     /locus_tag="PWY98_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2037077.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="PWY98_001317"
     CDS             complement(2567..3943)
                     /locus_tag="PWY98_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2037078.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="PWY98_001318"
     CDS             complement(4071..5189)
                     /locus_tag="PWY98_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2037079.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="PWY98_001319"
     CDS             5331..5765
                     /locus_tag="PWY98_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037080.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="PWY98_001320"
     CDS             complement(5843..6376)
                     /locus_tag="PWY98_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2037081.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="PWY98_001321"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="PWY98_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2037082.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="PWY98_001322"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="PWY98_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2037083.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="PWY98_001323"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="PWY98_001323"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2037084.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="PWY98_001324"
     CDS             complement(10101..10679)
                     /locus_tag="PWY98_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2037085.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="PWY98_001325"
     CDS             11001..11471
                     /locus_tag="PWY98_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2037086.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="PWY98_001326"
     CDS             complement(11579..12805)
                     /locus_tag="PWY98_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2037087.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="PWY98_001327"
     CDS             complement(13559..13759)
                     /locus_tag="PWY98_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2037088.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="PWY98_001328"
     CDS             complement(13893..14501)
                     /locus_tag="PWY98_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2037089.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="PWY98_001329"
     CDS             complement(14586..15056)
                     /locus_tag="PWY98_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2037090.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="PWY98_001330"
     CDS             complement(15104..16018)
                     /locus_tag="PWY98_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2037091.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="PWY98_001331"
     CDS             16164..16967
                     /locus_tag="PWY98_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2037092.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="PWY98_001332"
     CDS             17145..18092
                     /locus_tag="PWY98_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2037093.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="PWY98_001333"
     CDS             complement(18160..18453)
                     /locus_tag="PWY98_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2037094.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="PWY98_001334"
     CDS             complement(18481..19146)
                     /locus_tag="PWY98_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2037095.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="PWY98_001335"
     CDS             complement(19286..19918)
                     /locus_tag="PWY98_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037096.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="PWY98_001336"
     CDS             complement(19925..20992)
                     /locus_tag="PWY98_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2037097.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="PWY98_001337"
     CDS             complement(20989..21720)
                     /locus_tag="PWY98_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2037098.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="PWY98_001338"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="PWY98_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2037099.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="PWY98_001339"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="PWY98_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2037100.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="PWY98_001340"
     CDS             23883..25052
                     /locus_tag="PWY98_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2037101.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7575 a         5085 c         4059 g         8488 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttc
//
LOCUS       DANKAY010000033        24319 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000033 DANKAY010000000
VERSION     DANKAY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(122..667)
                     /locus_tag="PWY98_001341"
     CDS             complement(122..667)
                     /locus_tag="PWY98_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2037102.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(679..1371)
                     /locus_tag="PWY98_001342"
     CDS             complement(679..1371)
                     /locus_tag="PWY98_001342"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2037103.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1390..1674)
                     /locus_tag="PWY98_001343"
     CDS             complement(1390..1674)
                     /locus_tag="PWY98_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037104.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1687..2250)
                     /locus_tag="PWY98_001344"
     CDS             complement(1687..2250)
                     /locus_tag="PWY98_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2037105.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2478..3140
                     /locus_tag="PWY98_001345"
     CDS             2478..3140
                     /locus_tag="PWY98_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2037106.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3264..4460
                     /locus_tag="PWY98_001346"
     CDS             3264..4460
                     /locus_tag="PWY98_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2037107.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4561..5937)
                     /locus_tag="PWY98_001347"
     CDS             complement(4561..5937)
                     /locus_tag="PWY98_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2037108.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5974..6840)
                     /locus_tag="PWY98_001348"
     CDS             complement(5974..6840)
                     /locus_tag="PWY98_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2037109.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6929..7954)
                     /locus_tag="PWY98_001349"
     CDS             complement(6929..7954)
                     /locus_tag="PWY98_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2037110.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7972..8646)
                     /locus_tag="PWY98_001350"
     CDS             complement(7972..8646)
                     /locus_tag="PWY98_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037111.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8830..9723)
                     /locus_tag="PWY98_001351"
     CDS             complement(8830..9723)
                     /locus_tag="PWY98_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2037112.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9797..11494)
                     /locus_tag="PWY98_001352"
     CDS             complement(9797..11494)
                     /locus_tag="PWY98_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2037113.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11487..12800)
                     /locus_tag="PWY98_001353"
     CDS             complement(11487..12800)
                     /locus_tag="PWY98_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2037114.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12800..16837)
                     /locus_tag="PWY98_001354"
     CDS             complement(12800..16837)
                     /locus_tag="PWY98_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2037115.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16850..17308)
                     /locus_tag="PWY98_001355"
     CDS             complement(16850..17308)
                     /locus_tag="PWY98_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2037116.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17606..18124)
                     /locus_tag="PWY98_001356"
     CDS             complement(17606..18124)
                     /locus_tag="PWY98_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2037117.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18126..18742)
                     /locus_tag="PWY98_001357"
                     /pseudo
     CDS             complement(18126..18742)
                     /locus_tag="PWY98_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(18739..19602)
                     /locus_tag="PWY98_001358"
     CDS             complement(18739..19602)
                     /locus_tag="PWY98_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2037118.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19589..20500)
                     /locus_tag="PWY98_001359"
     CDS             complement(19589..20500)
                     /locus_tag="PWY98_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2037119.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20541..21875)
                     /locus_tag="PWY98_001360"
     CDS             complement(20541..21875)
                     /locus_tag="PWY98_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2037120.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22083..22913
                     /locus_tag="PWY98_001361"
     CDS             22083..22913
                     /locus_tag="PWY98_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2037121.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23157..23475)
                     /locus_tag="PWY98_001362"
                     /pseudo
     CDS             complement(23157..23475)
                     /locus_tag="PWY98_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23597..23791)
                     /locus_tag="PWY98_001363"
     CDS             complement(23597..23791)
                     /locus_tag="PWY98_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037122.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7148 a         5283 c         4211 g         7677 t
ORIGIN      
        1 acggaaaaag aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt
       61 ttgttttatg ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt
      121 tttataaaat ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa
      181 cgggatcgaa acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg
      241 catcgactgc tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg
      301 tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc
      361 gtttgtctat ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag
      421 agccgtacaa ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct
      481 ccggatgaaa ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt
      541 gatcaataaa cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc
      601 cagtagtcag ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg
      661 cgttcatctt agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga
      721 tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt
      781 gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac
      841 agcggctcct tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat
      901 tttatctggc gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa
      961 attcgttgct tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata
     1021 tagaggcatt cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa
     1081 ataagctact ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc
     1141 agctgaacct gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt
     1201 aatagatgct aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa
     1261 tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc
     1321 tctcaaaatt tttacttctt cttccattgc gccgataata ccgattttca tctttttaat
     1381 tccttctttc tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat
     1441 aataagagat ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct
     1501 tgttttcgtg attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt
     1561 ttcttcttgc tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg
     1621 tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg
     1681 atccctctac tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc
     1741 atcacaaatc aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa
     1801 ctccagtact tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc
     1861 atgataaata tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg
     1921 gctcagcgac ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc
     1981 gggattttgt ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc
     2041 taaaggtttt cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat
     2101 cccgacaccg ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg
     2161 aacttcgtct acagcaacat caataatttt tccgctatag atttctttgc gactgattgt
     2221 tttttcttca aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac
     2281 tgcttttttt tcatttttca atttttctca aaaactcctt attagtttac catagataaa
     2341 ccttagattt tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc
     2401 cgtatgctaa aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat
     2461 gacatggagg gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc
     2521 tatttctctt tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg
     2581 tagttttagt tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa
     2641 ttcctgcact gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg
     2701 aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa
     2761 tgaatatgca gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag
     2821 cagcgaaagc tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc
     2881 agtttcttta tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca
     2941 atgaccatga gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca
     3001 tcgaccagct gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag
     3061 atgatatcga cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg
     3121 aaccagaaca aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga
     3181 tgtctggctg tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca
     3241 gattcaacaa ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac
     3301 cagttaacga tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc
     3361 tgaccacttc acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt
     3421 taattgacaa actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg
     3481 atgctaatga tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg
     3541 aattctcaca atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt
     3601 ccttaactga actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag
     3661 aagggaacat cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag
     3721 caaaatatca aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa
     3781 aagacggtct gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact
     3841 actttgatgc actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa
     3901 caacgatcat tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc
     3961 agattgccaa tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc
     4021 gcttagctag acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa
     4081 atcaggcctt ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa
     4141 accaagtcgt gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc
     4201 aagtctctga aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag
     4261 ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca
     4321 ctcaaaatga tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag
     4381 aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac
     4441 tgttggattt aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca
     4501 gtttattaca actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt
     4561 ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat
     4621 aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat
     4681 acaagtcaag atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc
     4741 ttttggtttg ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata
     4801 ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa
     4861 gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc
     4921 gatttctgtc ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc
     4981 aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat
     5041 caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac
     5101 tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac
     5161 agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac
     5221 ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc
     5281 accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac
     5341 gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc
     5401 attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac
     5461 tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa
     5521 tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc
     5581 ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca
     5641 ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac
     5701 tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac
     5761 aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa
     5821 tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc
     5881 ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga
     5941 agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag
     6001 gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt
     6061 ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga
     6121 aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc
     6181 taagccccat aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt
     6241 acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga
     6301 catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga
     6361 gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt
     6421 tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat
     6481 aataccgtat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat
     6541 cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta
     6601 cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc
     6661 gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa
     6721 cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc
     6781 acttactaaa ccaatactac cccatgccaa catagggact aatgctatta aaatttccat
     6841 aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca
     6901 agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg
     6961 ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac
     7021 ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc
     7081 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
     7141 ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc
     7201 tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga
     7261 agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt
     7321 agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc
     7381 cttttagtcc atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat
     7441 acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact
     7501 tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca
     7561 ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg
     7621 ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt
     7681 tttcttcttc tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt
     7741 cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca
     7801 gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg
     7861 gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg
     7921 tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac
     7981 gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt
     8041 tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc
     8101 ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat
     8161 aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact
     8221 tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt
     8281 gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa
     8341 atgttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt
     8401 attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca
     8461 ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct
     8521 ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt
     8581 ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt
     8641 tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg
     8701 aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata
     8761 tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata
     8821 attggtctat tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca
     8881 ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata
     8941 aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg
     9001 tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt
     9061 gactagatcc tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat
     9121 tttctcaacg acttcataag cgagcatccg accatacact tcaagataga atcggtcatc
     9181 ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt
     9241 aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc
     9301 tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt
     9361 cggaatatag atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac
     9421 atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt
     9481 atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc
     9541 tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct
     9601 gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc
     9661 cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt
     9721 cattttcttc accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc
     9781 agcttatttt tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg
     9841 ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag
     9901 caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta
     9961 cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct
    10021 cacctgttgg aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat
    10081 aatctaaccc ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc
    10141 cgcttgttgc ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc
    10201 ctttataaaa tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt
    10261 taagcaattc actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt
    10321 tctctggcgg ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa
    10381 ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg
    10441 tgatcgttaa atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta
    10501 ctccattttc ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat
    10561 aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat
    10621 aagttagacc aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt
    10681 tttcaaggac agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt
    10741 ttcctctttc tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg
    10801 ttacttgtct aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt
    10861 ttttcggata caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca
    10921 cgccatccac catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc
    10981 tggaaatatg agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag
    11041 gtgcttcttc cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt
    11101 gtttgtcaat acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa
    11161 tccattcaat cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat
    11221 ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa
    11281 tattttcttc accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag
    11341 atgagaaacc ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt
    11401 cggctttgac tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta
    11461 cactcattcc cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt
    11521 aataaaacag cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca
    11581 ttcgtttgcg ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc
    11641 tctttcggtt tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat
    11701 tcataggcag agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact
    11761 actacatatt gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt
    11821 ctcttacttg cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag
    11881 gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat
    11941 ttgcttacat ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc
    12001 aagtcattag caggagccat atctaaataa tatttccccc cttctatcat ctgatataga
    12061 gcaaaaacag caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac
    12121 ttgaagaagt atggatctct tagataaccg acatttttag gatagatatg aatctcttgc
    12181 agttcctcgg ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct
    12241 gtctgatcga tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa
    12301 taaactgcat cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct
    12361 aatccgccaa ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct
    12421 tctgtgatta gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct
    12481 ttaggagtca tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg
    12541 gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct
    12601 tcctcagaga tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata
    12661 tctcgaatca tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc
    12721 gacatcgcat gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc
    12781 aaacacaaca gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac
    12841 cataggctgc tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc
    12901 cggatcctcc tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc
    12961 tgattcgatt tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg
    13021 cttcttcgtt tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa
    13081 ctacccgatc attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac
    13141 ctggaagttt tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca
    13201 acaagtctga agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga
    13261 tgatttcata ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac
    13321 gtcggctatt catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt
    13381 ctaaggttac ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt
    13441 cgtcttcgat gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga
    13501 acacttgatc agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac
    13561 caatagacag atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg
    13621 cacgagaata gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat
    13681 taccagtgta tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac
    13741 ctgcaccatc taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat
    13801 caaacgtatt gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg
    13861 tgagatattg ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat
    13921 cgtaaagtcg caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct
    13981 ggctctcaat gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa
    14041 atgaatctgg ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc
    14101 cgtcttcatc tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac
    14161 cagaaagccc tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa
    14221 gttcaaactc tgcgccattg attggcatat tactgccttc aattagttta ttgatattca
    14281 tgtggattgg cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg
    14341 tgccttctat gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg
    14401 aattcggatc tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa
    14461 gctcgtagtg acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat
    14521 acgtctcatc tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt
    14581 cgctgactgc tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc
    14641 cgtccgtggt agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt
    14701 catataatgc aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt
    14761 ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag
    14821 tagtgatatc attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc
    14881 aaaaatttcc gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt
    14941 ccccttcacc taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg
    15001 ttgtcggagt cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt
    15061 ctggctcttg gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg
    15121 ccgatctgcc agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt
    15181 gctggatcgt tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct
    15241 tgttgttcgc attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt
    15301 tcaatacatt tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc
    15361 ctaaaccaat ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat
    15421 gataagcaac tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat
    15481 tgttattaga agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct
    15541 ctccataacg atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg
    15601 taactggatg agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga
    15661 acaaaacgtc attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc
    15721 cattagtgct cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat
    15781 ctcccacaac gattaggatt ttttctggat ttctattttc tccgccattg tctttataca
    15841 atgtttcata gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat
    15901 ttaatgctgt attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt
    15961 ccgaactgat ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac
    16021 cgattcgaat atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct
    16081 caatcaacgc agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg
    16141 ctgacttatc tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga
    16201 tgacatctag atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg
    16261 tgctgcttgg cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt
    16321 aactgttttc aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc
    16381 tatcttcttc gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg
    16441 ctcccccgtt tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt
    16501 tgtctccttg atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat
    16561 tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat
    16621 attcataggg gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact
    16681 ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca
    16741 tctcattttc ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt
    16801 gtaattcttg gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt
    16861 tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat
    16921 aaacttttcc gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat
    16981 cttcatcata ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg
    17041 ctgccgcatc gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag
    17101 ccagatgcca ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt
    17161 cctcatctaa gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg
    17221 tcgtcatatg atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact
    17281 gcaaaagctt caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt
    17341 ctttcacaac aatcaacaaa acaatgccca tctaattata atttaattta attttaattt
    17401 taaatcaaat gatatccttt ttagttaaaa aacaccaatt atacaacatt tattattaaa
    17461 attaaattat aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat
    17521 gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca
    17581 ctcatgcttt attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac
    17641 ttcctaaaat agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg
    17701 aataagcgat caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga
    17761 acaacgcagc tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct
    17821 tcggtttact tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg
    17881 tcgttttttt gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg
    17941 ctagaccata ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa
    18001 taaaaccacc attagagata ttactgataa atttcaaaaa attgattttt cgactatccg
    18061 ttttccgttg cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca
    18121 acatgttatt cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact
    18181 tgaaaataaa acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag
    18241 aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta
    18301 aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt
    18361 ttgtaactaa agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta
    18421 tagccaattc ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa
    18481 taaacacttg ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc
    18541 cagaatagtg aaccgagctt tttctcttca tttttttgta atgataaatc atatacagaa
    18601 ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct
    18661 gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt
    18721 taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa
    18781 ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc
    18841 catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa
    18901 cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc
    18961 gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg
    19021 actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt
    19081 gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg
    19141 aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg
    19201 gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt
    19261 tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt
    19321 accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa
    19381 ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg
    19441 ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat
    19501 gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt tgtttgccga
    19561 ttcacactgg tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc
    19621 acttaggtgc aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca
    19681 ttgacatagc catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca
    19741 gattataaaa aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag
    19801 cttcttgaaa aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag
    19861 gagtgattaa tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt
    19921 ctgctgattc atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg
    19981 ctcctccaga actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa
    20041 acagccaagc tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag
    20101 atggagaaag cagctgcatc cataagatgt agactgctac tcctgataag actgccggta
    20161 aatagaaagc tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta
    20221 aaaacacact gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt
    20281 ataacgattt ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa
    20341 tgaaattcat cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca
    20401 taggccctag cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc
    20461 atcttgcttc ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta
    20521 cctagacagt caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt
    20581 gcatctgcca atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca
    20641 ttcactaatg tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta
    20701 aagttttcgt ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc
    20761 actaaattct cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt
    20821 tcttgtactt ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt
    20881 gctccgtatg gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct
    20941 tctttttctg ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa
    21001 ctagagtaat aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca
    21061 gatacaccag acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct
    21121 tgccagtctt ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt
    21181 tcatctttgt caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct
    21241 aaattccata atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt
    21301 ttacgtgctg tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca
    21361 gcttctttaa atatcttttt attataaaaa atgacttgtg tatctgtatt gaatgggatc
    21421 gcataaggat caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa
    21481 gtgtcttttt ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca
    21541 acggaattta tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa
    21601 gatttagtcc agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt
    21661 gattgattga aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa
    21721 aaggaccaga aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca
    21781 cttgaactgg catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa
    21841 cttgccagca accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa
    21901 ccgatgccgg ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta
    21961 tgattctaaa aaaattatta atgataccaa tactttatat aattcgcgag cgctttcttt
    22021 gaatttttta atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt
    22081 tgatgtttag acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat
    22141 taatcgcgaa atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta
    22201 tgcagtacct gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga
    22261 tggacattgc aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt
    22321 tattagaggg tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg
    22381 gtgccattat ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc
    22441 ctgctgacag cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt
    22501 tagcagactt tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta
    22561 tttacgaaat gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag
    22621 atagctatca tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt
    22681 caatcttagt cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact
    22741 tctataactt ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac
    22801 tggatggcga aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa
    22861 aattagaacc agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta
    22921 cccgtagaaa cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat
    22981 taatcataac cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg
    23041 ccagcgccta aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt
    23101 tttgcccacc tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac
    23161 tctcgtacac tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt
    23221 ttgatggtcg cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt
    23281 cgatacccaa atttatattt tagatttgca caatctcttg atgatctcct gaatattggc
    23341 tcgatattga ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt
    23401 acacatctac tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt
    23461 ggttttgtat ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa
    23521 cccgctgctt cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct
    23581 cttacggcaa tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc
    23641 caagaaataa agccgtataa aaacaagaac ctatgaaatc tttatttatt agatattcaa
    23701 cagaaatccc gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct
    23761 ttttatagaa tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta
    23821 atcacaaatt cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct
    23881 tcgactttcc ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac
    23941 ctctaaatct aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca
    24001 tccagtatac ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc
    24061 cattgcagga gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat
    24121 aggacttact cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc
    24181 aactacatgc acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga
    24241 atcgggaatt gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata
    24301 tctttattat aaaataacc
//
LOCUS       DANKAY010000034        23969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000034 DANKAY010000000
VERSION     DANKAY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            31..663
                     /locus_tag="PWY98_001364"
     CDS             31..663
                     /locus_tag="PWY98_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037123.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(797..922)
                     /locus_tag="PWY98_001365"
     CDS             complement(797..922)
                     /locus_tag="PWY98_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037124.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1056..1949)
                     /locus_tag="PWY98_001366"
     CDS             complement(1056..1949)
                     /locus_tag="PWY98_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2037125.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2131..3057)
                     /locus_tag="PWY98_001367"
     CDS             complement(2131..3057)
                     /locus_tag="PWY98_001367"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2037126.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3142..3903)
                     /gene="pflA"
                     /locus_tag="PWY98_001368"
     CDS             complement(3142..3903)
                     /gene="pflA"
                     /locus_tag="PWY98_001368"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2037127.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4147..6378)
                     /gene="pflB"
                     /locus_tag="PWY98_001369"
     CDS             complement(4147..6378)
                     /gene="pflB"
                     /locus_tag="PWY98_001369"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2037128.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6655..9105)
                     /gene="parC"
                     /locus_tag="PWY98_001370"
     CDS             complement(6655..9105)
                     /gene="parC"
                     /locus_tag="PWY98_001370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2037129.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9117..11162)
                     /gene="parE"
                     /locus_tag="PWY98_001371"
     CDS             complement(9117..11162)
                     /gene="parE"
                     /locus_tag="PWY98_001371"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2037130.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11328..11762)
                     /locus_tag="PWY98_001372"
     CDS             complement(11328..11762)
                     /locus_tag="PWY98_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2037131.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11889..12527
                     /gene="plsY"
                     /locus_tag="PWY98_001373"
     CDS             11889..12527
                     /gene="plsY"
                     /locus_tag="PWY98_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2037132.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12659..13534)
                     /locus_tag="PWY98_001374"
     CDS             complement(12659..13534)
                     /locus_tag="PWY98_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2037133.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13616..14407)
                     /gene="codY"
                     /locus_tag="PWY98_001375"
     CDS             complement(13616..14407)
                     /gene="codY"
                     /locus_tag="PWY98_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2037134.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14425..15825)
                     /gene="hslU"
                     /locus_tag="PWY98_001376"
     CDS             complement(14425..15825)
                     /gene="hslU"
                     /locus_tag="PWY98_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2037135.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15839..16387)
                     /gene="hslV"
                     /locus_tag="PWY98_001377"
     CDS             complement(15839..16387)
                     /gene="hslV"
                     /locus_tag="PWY98_001377"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2037136.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16424..17329)
                     /gene="xerC"
                     /locus_tag="PWY98_001378"
     CDS             complement(16424..17329)
                     /gene="xerC"
                     /locus_tag="PWY98_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2037137.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17527..18903)
                     /gene="trmFO"
                     /locus_tag="PWY98_001379"
     CDS             complement(17527..18903)
                     /gene="trmFO"
                     /locus_tag="PWY98_001379"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2037138.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18872..20950)
                     /gene="topA"
                     /locus_tag="PWY98_001380"
     CDS             complement(18872..20950)
                     /gene="topA"
                     /locus_tag="PWY98_001380"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2037139.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21072..21929)
                     /gene="dprA"
                     /locus_tag="PWY98_001381"
     CDS             complement(21072..21929)
                     /gene="dprA"
                     /locus_tag="PWY98_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2037140.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21985..22752)
                     /locus_tag="PWY98_001382"
     CDS             complement(21985..22752)
                     /locus_tag="PWY98_001382"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2037141.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22745..23605)
                     /gene="ylqF"
                     /locus_tag="PWY98_001383"
     CDS             complement(22745..23605)
                     /gene="ylqF"
                     /locus_tag="PWY98_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2037142.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6257 a         5149 c         4118 g         8445 t
ORIGIN      
        1 atatacttat caatttaatt tggagatatt atggaaattc aacgtagaac cactaatttt
       61 ttacatttag gctctttttt tgcaactttg acgatcttat tttttctatg ttattcatac
      121 cttaagacct cacatgcgcc ttctagtgca ttaggctggt tgcttttgga tcattctgat
      181 atcacagaag ccgaagaaaa tccgtttttt ttgattttgt cttctctttg cggcttactg
      241 ttttctgttt atttcggtgc ctattttttt catcgacatc ctggtacgtt aaacgaagct
      301 tcaagcgtat tgattgcatt taaaacgttt tcttttttag caattctttt ttattcgttt
      361 aatcacaata cgcttttctt ctttatcatg aatggtttag tcgtgatttt atccattttt
      421 acttatttta ttttttcact catttttagc caattgaaat gtccgctctt ctttcgatta
      481 gttcctgtca cctgttttat ttacagcttg ttttcctttt ttgtccttgc tttaatgcca
      541 ctgaccaacc cttcgacgat ccaagtcgca ggaaatctgt tggatatgct cttcatcgtc
      601 agtctaggtg tatttatgtg gcaaagaaaa aacaaacaaa ataaaaagca ttttgaagcc
      661 tgactattac gacttcataa atgctttttt agttttaaac acttcaatct aaaagaataa
      721 cccaagattg gataaattat taatgactcg ccattcgttt cttagtgatt ctggattatc
      781 aaatggactt cctaatctaa tcagctagtt cttcccataa aatgccatac ttgactgaat
      841 cataatattg attatttaaa aatctaactt ttcttatcac tacttccttt gtcattccac
      901 atttctcacc atttttttgc attcctttgt ttccagacca tgtagtgaat ctaataggtg
      961 gtagataggc aaaatactca aataattctt ttaaccataa aggaagagaa aggctcctcc
     1021 tgcaagagtc agtataattt ttgcttactg ctctcttagg ttttactcac taaaactgtt
     1081 ccatattctt tgatataaga aaatgcatgc ggtataaatt tcttgctcag ctcagaaaag
     1141 atttgtcgtt taagaaaggc tatttcctcc attgtcaatg aaggatccaa tgttggtaat
     1201 tcaatctcaa aattgttttt ttcgacaata ggtaattcta atgtgaatga ttcttcttcc
     1261 tttatagtat tcgtcacaag tagagactga tggaaaatac taggccggcg atttttatat
     1321 ttattcttac gtatcctgtc aacgataact tctatttgat caaaaaagta cgattcattt
     1381 acgtctccaa taagttcggc taattctttg aatgccttcg ttacaggaag ctgattcttc
     1441 tgtacaaaaa agtaattagc caaagtaagc gggaagtgtt ctatagagga aagtgtgata
     1501 aaacggtact tctcatttcc tgcagttgtc ctcagcaatt ctgtctctcg gctgtttctt
     1561 actccataaa aatacgcttc tttccgttca tcttcgaaac aatacgccca aataacttct
     1621 cccatcgcta atttttgttc atcaacagag agtctcttca attgttcagc tatggtttca
     1681 gcagccgaag tggcttgttg caagtaatct ttaggatcaa atattggaaa gttcaaatga
     1741 tatcttcgtt cttccctctt gattaatcct gcatcgatca ataaatcaag aaaatgggaa
     1801 aatttatttt gcgaaaattc ttttttcaat tctcttagaa tcgtttcttg ttctcgatgt
     1861 tcatatagat aatcggtcaa tgaagaaaaa agcggatcat cggtcagttt ttgataaaag
     1921 ttttccttat tttttgaata atagttcata tatgttctct tttctatccg ttttatttta
     1981 cttaaaaggc ttttacgtaa aaaggaggaa gaacaagagt cataattgac tgttattctc
     2041 ctccatttct tcggccttta gtatgttcta tgtttctttt tatatcaatc gcttatccat
     2101 tcattttgaa tgcttcatac taccatttga ttagctaaat acagccgtca attgaggtac
     2161 gacttgtttt ttacgtgaaa cgacaccttt aagcaaagca cggttgttgg tcaaagttgt
     2221 ttggaaagct tcttctactt tttcttttcc tttaccagca acaagtaatt ctgaatcact
     2281 gtctaagatg tttgtgacaa ctaatacgaa cagatcgtag ccatttgctt tcatttcatc
     2341 ttccattgct gcttctagct ctgcttgtcg atccatcact tcattaaggt ccactgtatt
     2401 gacttgagcg atacgaacgt tggaactgcc cattgggaaa ctttttgcat ccaaatcaag
     2461 aagaaccgca gctgatttat cacttagatt tgttcctgct ttcaacattt caagtccata
     2521 agtgtttaga tcgacttcag cgattgctgc aagttctttt gcagctgctt catcttcagg
     2581 cgtacatgtt ggtgatttga acaacaatgt atctgaaatg atagctgaga gcatgattcc
     2641 tgcgatttct ttaggaattg ttacttggtt ttctttatat agttttaaaa taatcgtact
     2701 tgtacatcca actggttctg cacgatagta caatggatta gctgtttcaa agtttgcaat
     2761 acgatggtgg tctactacag ctaaaatatt tacttcgctg atatctgaaa tactttgttg
     2821 gaattcatta tgatccacaa gcatgacatc tgcattttct gctttagctg actctactac
     2881 tcgtggtgca gatagctgaa agtggtctaa tgcgtattgt gtttcttcat taggtgtacc
     2941 tagagcaact gcttctgttt ctttacctaa ttcattttgc aagtgtgaaa acgcaatggc
     3001 tgccccgatt gcatctgtat ctggattttg gtgaccaaaa actaaaattt ttgacatgtc
     3061 attcgctcct ttgtgatttc tcctatttaa taatagcaaa aaaacggaaa gaagcaatac
     3121 ttatcttctt tccgatgcga cctatcgcgt taaatatttt gtatactctt ctgtatgaag
     3181 caatcgttta gcattttcga cacgttcttt tgtaggtggt tcgatccctt ctaatggata
     3241 tttcaaacca agtgtttccc acttgtattt tcccatcgta tgataaggaa gaatctctac
     3301 tctgtctaca tttgacaagg tttgaataaa gttgtctagt cggatcaaat attcatcata
     3361 atcacttcgc tcaggaacta gtacatggcg aatccaaacc ggtttgttga tttttgacaa
     3421 atattgagcc atttctaaaa tattttcatt gtcccaatgc gttagttttt tatgctcttc
     3481 attatcgatg tgtttgatgt caaataacag taaatctgta tacttcatca attcttgaaa
     3541 acgtgaaaag aaaggttctt catacgtaaa aggttttccg catgtatcca atgtagtatg
     3601 catcttccgc tcttttgctt ttttaaatag ttcgatcaaa aaatcgatct gcaatagagg
     3661 ttctccacca ctaacagtaa taccaccttt atctccccag taaggtctga agcgttctgc
     3721 tttatctaaa agttcatctg ctgtgtattc tttccctccg ccaatattcc aagtatctgg
     3781 attgtgacaa aattggcaac gcatgcggca tccttgcata aacacgacaa agcgcagtcc
     3841 aggtccgtct actgaaccga aagtttcgat agaatgaaca tagccgattg ttttttcttc
     3901 catttaagtt cacatctttt ctttgatttc attttaagta caagttagct gtcgaagcga
     3961 agctttctga acaaaatttt tcgcaattgc cgcttcgtta tgtgtaaaat ttacagggac
     4021 ggaacataca agtcctgccc ctgtaaatcg tttctttcaa ataagcatac tgattattcg
     4081 attttgatgc gatggctatt caacgtcctt tcgacgctaa gtcgaaaaac ttttttcacg
     4141 aactttttac attctgtcgt gtgaagttct catcaagaca tctaattgtt gttctcttgt
     4201 caagtcacgg aatttaactg cataaccaga tacccggatc gtcaagttcg gatatttcat
     4261 tggattttcc attgcatcac gcaataaatc attactgaat acattgacat tcaaatgata
     4321 agctcctttt gagaagtatc catctaagac attacgcaag ttttcgattc ttgtatcttg
     4381 ttcttttcca agacctgaag gattgattgt ttgggtattt gaaattccat ccaatgcaca
     4441 gccgtaattc attttagctg ttgagttcag gctagctaat aacccacttt gttccgcatt
     4501 gtaacttgga tttgctcctg gcgcaagtgg ttttccagct tcacggccat caggagtatt
     4561 tcctgtattt ttaccgtaaa caacatttga agtaatcgtc aataatgaag ttgttggttt
     4621 tgagttgcgg taagttttat gacgtttgat ttgtcccatg aagtattcca agatccatga
     4681 aactaattca tctgcacggt catcatcatt tccgtattta gggaagtcgc catccacttc
     4741 aaaatcaact gctattcctt tttcattacg gattgctttt acttttgcat atttgatcgc
     4801 tgacaagctg tctgccgcat gagagatccc agcaatacct gtagcaaatg tgcgttgcaa
     4861 gtcagaatcc atcaaagcca gctgagctgc ttcataagaa tatttatcat gcatataatg
     4921 aatgatgttc aacgtattga catatagttc tgctaaccaa tccatcatat tcatgaagtt
     4981 atgtttcact tcttcaaagt ctagtatttc tgatgtgatt ggacgatatt ctggtccaac
     5041 ttgggcgcca gtcacttcat cgactccgcc attgatggca tataatagtg ttttagccaa
     5101 gttagcacga gcgccaaaga attgcatgtc ttttccgaca actgttcctg atacacagca
     5161 agcaatcgca caatcatcag aaccccattg ttcacgaagc tgattatcat tttcgaattg
     5221 aatcgaagaa ctttcgatag cgattctgct agcatatgtt ctgaatcctt ctggaaggtg
     5281 ttcagaatac aaaacagtca agtttggttc tggtgaaggt cccatatttg ttaatgtatg
     5341 aagcagacgg aaatcattct ttgtaactaa tgaacgacca tctaatccca tacctgcaat
     5401 ggataaagta gcccagatag ggaatcctga gaataattgg ttatattcag gtgtacgcgc
     5461 gaatttgacc atacgtaatt tcattactaa gtggtcgatc aattcttgtg cttctttttc
     5521 tgtgattact ccattatcca aatcacgttg gatatagata tcaaggaatg ctgagatacg
     5581 tccgattgac atcgcagcac cattttgtga tttgattgct gctaaatatc cgaagtatag
     5641 ccattggaca gcttcttttg catttgcagc tggtttagaa atatcatagc catatttcgc
     5701 agccatttct ttcatttgcg ctaatgcacg atattgttca gtgatttctt cacgtaaacg
     5761 aatcacttct tcactcattg tatgataacc agtcatatca tgatcttttt tcttttgttc
     5821 catcaaatag tcgattccat acaatgccac acgacgatag tcaccgatga tacgtccgcg
     5881 accgtaagcg tctggaagcc ctgtaatgat tttgttttta cgagcagcac gcatttcagc
     5941 agtataagcg tcaaatacac cttgattatg tgttttgcgc cagtcagtga aaatatgtgt
     6001 caattcatcg tttggtgtat agccattgct ttttagcgca ttgttagcca tattgattcc
     6061 gccgaacggc atgaatgctt gtttcaaagg tttgtctgtc tgtagaccga cgattttttc
     6121 caaatcttta tccaaatagc ctgcatcgtg tgaagtaata gttgatacga tttctgtgtc
     6181 catatcatag acaccgttac gctcgaattg gatatcattt aacttttgca attcatccca
     6241 taatttttct gttgcttctg ttgggccttc caaaaagctg tcatcaccgc gatattcagt
     6301 gtagttattt tgaataaagt ctctagtgtc tacgccttct ttccatttgg tacctttaaa
     6361 tccattccat tgctccattg tatggacctc ctaaatttct gttttatgta acagttttta
     6421 cgtgattagt ataacacagc accctatctt tgcaagggtt ttcttgtttt ttatttaaca
     6481 atttttatgt tttatttaca tatatttaaa tcaacgttga cataccaaca tttaagcatt
     6541 gtgaacactt tcatcttttt ttgcaaacgt ataaggattt attaaaaatg aatgaaaaag
     6601 agacgccacc tgtattaata cagatggcgt ctcttcatta aaatactaca acagttacga
     6661 ctcgatgaaa atctcaggag catcgataac ttcgatgact tctccacctt ttttctcatc
     6721 catcgcaaat gatccattcg atgttcgatc acctataggg aaatcagttg ggtcaatcgt
     6781 taactgctgc cctttttgat tcatcaaggt tatttctttt gatcttcctt ccgacatata
     6841 gataatacgg tgcggatttt ttttcagctc acgcatgacc gtcactcccc gcttcgcacg
     6901 tgaagtttgg ctgatttctt gagcaagcat gcgtttaaca gctcctcgct gagtaacgat
     6961 tacgatcggc gtatcacctt ctgctaacac aagcagcccg ttgataatcc tatctccatc
     7021 tttcaaattc atagacttca ctccagctgc ctttgctcca acaacgggaa catctgttaa
     7081 aggatagcgc aaacccattc cttgatgcgt tactaagaaa acatctaagc ctggctgatc
     7141 ttctttcaga tacacagcaa tcagttcatc ttcttccgcc tttagcttca tacagctgag
     7201 cggacggctc ttataggtgc gccatggttc gaactctgtc attcgtgttt gcttgatcaa
     7261 tccattcttg ctgatgaata caaatgtttt ctctgcttga attttttgat atgggaaaac
     7321 agctaaaatc gattcgtcta cagacagatt ggagattgtt tgagaaatgt gctcaccagc
     7381 atctttccat ttcaaatcgg gtagttcatg gacagggcgg tagatcacat ttgctttatt
     7441 tgtgatcaga agaacatggt ccaatgtact caattcacct gagtatagca agtagtcgcc
     7501 ttctcgcatc ccgatttctt ctggtttgga agcactataa gaacgaaggc ttgttcgctt
     7561 cacatagcct tctcgcgtca cactgacaat tacgtcttct tgtgccacta agacttgcgt
     7621 atcaattttg atttcttcga tttcgtcttc gattgtcgtt agacgagcgg aagaataatt
     7681 ttttttcact tctcgcaatt cttttttcat aacagagaaa agctctttgt catttgaaag
     7741 gattttattc aactctgtga tttgcgcaat caagctttcg gattcctttt gaagctctgt
     7801 gatatccgtg ttcgtcaaac ggtataattg caatgtgaca attgcttctg cttgttcttc
     7861 tgtaaattga aagacgtcga ctaaattctt tttggcgtct tttttgtctt tactttcacg
     7921 aatcgttgcg attacttcat ccagaatcga taaagctttc atcaatcctt ctacgatatg
     7981 ctgtcttttt tgagctttgg ctaaatcgaa ttgactacgt tttgttatga cttgctttcg
     8041 atgctcgata taacttctta aaatgtcttt cagtccaact tgatgcggag tcatatggtc
     8101 gatagcgacc atattgaaat tgtaattgat ctgtagctca gtgtttttga acaaataatt
     8161 caaaattcct tgagcattgg cgtctttttt caattcgacg acaatttgca gacctgttct
     8221 atcactttca tctcgtactt cagctattcc atcgattttc ttattcaaac ggatctcatc
     8281 catttttttc accaaggtcg ctttatttac ttcatatggg atttctgtga tcacgatttg
     8341 ctgtttatta cccttcattg cctctatctt tgttttcgaa cgaagaataa cttttcctcg
     8401 tcctgtttca taagcttttt tgatttcatc ttttccttgc aagatccctc ctgttggaaa
     8461 gtcaggtcca ggaatatatt ccatcaattt ttcaaggctc gcttgtggat gatcgatcat
     8521 atagattgtg ccatcgatca cttcagctaa gttatgagtc gggatttctg ttgcatatcc
     8581 agcagatata cctgtcgatc cattgacgag aaggtttggg tatcgagctg gaaggactgt
     8641 tggttctttt tctgtatcat caaaattcca cacgagatcg actgtttctt tctcgatatc
     8701 tgctagcatc tctccactta acttggataa tctggcttca gtataacgca tcgcagcagg
     8761 tggatcaccg tccatactcc cgttattccc gtgcatttcg atcagtactt cccgcaactt
     8821 ccagtcttgg ctcatgcgga ccatcgcttc ataaatactg atgtcaccat gaggatgata
     8881 attccccatg atatttccga cagattttgc tgatttacgg aatcctttgt catacgtatt
     8941 gccatctttg ttcatggcaa ataatatccg gcgttgcact ggcttcaatc catcacggat
     9001 atcaggcagt gcacgttctt ggataatata tttggaataa cgtccgaagc ggtcacccat
     9061 cacttcttct aaagttaatt cttgtacttc ttgtcgattt tccatcaggt gacctcctat
     9121 tctacttcaa ataactgatt gttttcattt ttatctgctc gttcttcatc taataaatca
     9181 ttagaaactg aaggagagat ttcagtatcc tcttttttat ctaagatact gccatcttct
     9241 tcgagcgtaa attgcacgtg attttcgatc cattttcttc gaggttctac tttgtcaccc
     9301 atcagtgtgg ttactcgacg ttcagcttgc gcagcatcat cgatacggac gcggatcaac
     9361 gtacgggaag tcggatccat cgttgtttcc cacaattgtt ccgcattcat ctctccaagt
     9421 cctttatatc gctgcagcat atatcctttg ccgacttttt taatcattgc tgccaattcg
     9481 tcatctgtcc acgcatattc aatgacttgc ttctttcctt gtcctttgga tactttatat
     9541 aaaggcggta acgcgatata tactttccca gcttcaatca gcggtttcat ataacgataa
     9601 aagaaagtaa gcagtaatac ttgaatatgg gcgccgtctg tatccgcatc ggtcatgatg
     9661 attactttgt cgtaattaca gtcttcgata gaaaactcag gaccgactcc tgcaccaatc
     9721 gtatagatca tcgtattgat ttcttcattt ttcaagatat cttgcatttt agctttttcg
     9781 gtattgatga cttttcctcg taaaggtaga attgcttgga actttctgtc tcttccttgt
     9841 ttagcagatc cgccagcaga atccccttcg actaaataca attcattttt cttagggttt
     9901 cgggattgtg cgggtgtcaa ttttcctgaa agaagagatt cgcctttttt acgcttcttc
     9961 ccatttcggc tttcttctct ggctttacgt gctgcttctc tcgcttctct ggctttgatt
    10021 gctttacgga ccaacatctg gctcatttcg ctattttcct gcaaataaaa gcccatttgt
    10081 tcactgatga cgttatctac tacggtacga gcaactggcg taccaagttt ttcttttgtt
    10141 tgcccttcaa attgcagaag attttcaggt acacggatag aaagtacagc agctaaccct
    10201 tctctgaaat cactaccttc cagattcttg tcccgttctt tcagcaaacc aacttttctc
    10261 gcatattcat tataagattt cgtcatcgcc gctttcatac cagcttcatg ggttccccca
    10321 tcttttgtcc gaacgttatt gacaaaagat aaaacatttt ctgaataacc gtcattgtat
    10381 tgataagcga cctctacttc gataccttct ttttctcctg aaaaataaac aactggtgtc
    10441 aatgtatctt tttcttcatt cagataatca acaaattctt tgattccttc ttcgtaatga
    10501 aaaatttcct tgactggttc ttctccacga agatcagaaa gagagatttt aacgcctttt
    10561 agaagaaagg cagattctct taaccgttcg gctaaaattt catatgaaaa tttcgttgta
    10621 ctgaatattg tatcatctgg taaaaaatgg acagtcgtcc cgtttgcttt ttttgttttt
    10681 cctatctttt tcaaagtacc gtcaggttta ccgccgtttt taaatttctg ctgatactct
    10741 acgccatctc taacgatcgt tacaattagc catttcgata aggcatttac cacactggct
    10801 cctacaccgt gcagtccacc agaagttttg taccctcctt gaccaaactt tccgccagca
    10861 tgcagcacag taaaaatcac ttcaactgtt ggtattccgg aagcatgcat tcctacaggc
    10921 atcccacgtc cgctatctgc aacagttata ctattgtctt catggatggt cacatcaatc
    10981 tcactgccat aacctgataa tgcttcgtca actgcattat cgacgatttc atagactaaa
    11041 tgatgcaagc ctcggctgtc agtggatcca atgtacatcc ctggtctttt tctaactgcc
    11101 tctaatcctt ccaatacttg gatggacgag tcattgtatt cgttgtttac tttttttgcc
    11161 aaggcaaacg ctccttcaat aatactttgc ggcaaaaccg caccacgatt atcatactag
    11221 aaaaggtcgt gaaaaaaaag agggtagacg agaaatcaat tttaattatc tcctaaccgc
    11281 tttttcgaca ttcccctcta tcgatcatct cttctttgtg gtcattttta tttttggttt
    11341 tctttcaact taccaagttc aatcttaata caacgatcca ttacgatatc tgtacgtcct
    11401 gcttttctta gcatctcagc tgcttctttg ttttcaatcc ctaattgtgc ccagaacact
    11461 ttagcatcag ttttcaaaaa atcacctgct atttcaggta aaaattcact ccgtctaaat
    11521 acatctacga tatcgattgg atcatcgatt tctgtcaaat tcgcatagac tttctcgcct
    11581 agaatttctt caccagccaa taatggattt actggtatga ttttatatcc attgtcttgc
    11641 aatacctgtg caaccatgta acttgttcgt tctggtttat tgctcaagcc tacgacggca
    11701 atattttttg cttcttccaa atagttgaaa atcgtttctt gtgttggatt ttttacagac
    11761 atttctgcca ccccttgctt ttctttttat tccatctatt atacttgtat ctttcatttt
    11821 ttcaattgtt cctgttacct attcatgcta aaataaagga tagttttcaa cttgaaagag
    11881 ggtaaaaaat gaaattgatc ttattactta ttttagcata tcttttaggt tctattcctt
    11941 ccggtgtctg gattggcaaa ttgtttttca aaaaagatat ccgacaacac gggagcggta
    12001 atactggaac gaccaataca tttcgtgtat taggcaaaac agccgggatt gttgtcctgc
    12061 ttatggatat tttaaaaggt acattggcta cttgtttgcc gatgatcttc cacctttctg
    12121 ggattaaccc tctttggttt ggggtatgcg ccattcttgg ccatacattt ccgatttttg
    12181 ctgggttcaa aggaggaaaa gctgttgcga caagtgcagg aatgctttta ggatttaatc
    12241 ctgtattttt tgtttattct tgtatcatct ttatcgttag tttattttgt acaagtatgg
    12301 tgagtttgag cagtatgatc agtgcagtat tgatcacttt atctacgatc gtccttcctt
    12361 atgtagctcc tgtgattttg gctaagccca attggttact gacgatcatt gcctttttag
    12421 tttcttcctt tatcttttat cggcataaag acaatatcaa gcgtatcaga aaccatactg
    12481 aaagtcgaat cccgtttggt ttgaatagca gcaagaaaaa atcctgatac tttttcattc
    12541 caaagagata aaacgggagt gtgacaaatc gatgtcacac tcccttatta cttacaggat
    12601 agctttttta cacaccttgg taaacggtgt tccctgctaa cttctcggtc acaacctctt
    12661 acttcactgt gatggaatat tttgtcttga agatttcatg ggcacctagt tgattgatcc
    12721 ctactttttc catcaagttt ccactactgt tcaccgtgtc agcgattccg caccacggtt
    12781 cgatacaaac gaaaggcgct tcttttggat aaggagacca gatccctaca taaggcatat
    12841 ctttatagct taaagttacg ctatgggaag atttttcact tcgaatggcg tacgcattta
    12901 gaccccttgt ttcaaaaacc aaggcatctt gctcaaaaag ttcacgattc aaagccagat
    12961 tcgtatttgt ctgacctaac gtcttttgat ccatatcgat gaatgggcct ttcaatggta
    13021 ggcgtaatct tgatttcaat ggagaaaatg ccagatagaa atcttcaaat gacaatcctt
    13081 cttcaagagg tacattgaaa gcaggatgtc caccgataga aaagaacatt tctttttcac
    13141 cggtatttgc cacttgataa tgaaccgtga tgccgtctcc tcctaattca tagctgatca
    13201 ctaattcaaa atcaaatgga tacttttcta atgtctccgg cgtactcctt aagatgaaac
    13261 tagccatttc ttgtccatgt tcaatgactt caaaatccat atcccgagca aatccgtgtt
    13321 gccccatttg atatgttttc ccttgataaa catactgatc atcttttaaa cgtccaacaa
    13381 aagggaataa aaccggtgca tgtctgcccc aatacgctgg atcaccttgc cagatatact
    13441 ctaaaccatg ttcttttgat ttcagactga tcaattccgc accgtgttca gcgatttcgg
    13501 cgatcagctg gtcattttga ataataacag tcattgccat tcctccaact tcgacttact
    13561 ttttaaaatg ggaaggctgg aacaaacatt tctatctgcc ccagtcccct tacttctaat
    13621 aactgtgttt tgctaactcg tctttaaaac gggaattcaa tacttttaaa taagttccct
    13681 tcatgcctaa cgaacgagat tcaatgattc cagctgattc caattttcgt aaagcattga
    13741 cgatcacaga acgagtgatg ccgatactat cggcaatatt agaagcagtc agacgacctt
    13801 catctccatc caatgcttca aagatagctt gtacagcctt caattcgcta taagataatg
    13861 tgttgattgc catttggaca gccgttgcac tccgtacatc tgcttcgatg cttcgagact
    13921 tctgataaag gatctgcata ccgacaaccg tagctccata ttctgctaag actagatcgt
    13981 catctgcaaa cgattgttcc atacgagata agataatcgt acccaaacgt tcgccagctc
    14041 caaagatcgg aacgacagtc gtcagaccaa atggatattt ctctcttgac tctaccggaa
    14101 atgcagtaat atcactagta attgggatat tagcttcagt tttcatcaac tgtgccactg
    14161 tatcagtata agattcagga aattgtttct caacaaacat tgttttgatt cgttcattgt
    14221 tcacatcgtg acgttcatta taacctagta atactccgtc acttcctatg atataggtgt
    14281 tgctgtcaag aataccacct agtgtgacag ccattttatc gtaaggcaaa tctgcttgca
    14341 tgtcaaaagt attcttttgc tgcaaaagtg tgtttatctt gcgggttttc gttagcaagt
    14401 ctgtcattgt cgtcctccga ttttttataa aatatagcga cttaaatctt catttgccgc
    14461 aattgagcca agtttgtcat ccacatatgc ttctgtgatc gttatttctc ccatttgcat
    14521 atctggtgct tcaaataata gatcttctag tagtttttcc aaaatggtat gcagtctgcg
    14581 tgcgccgata ttatctgtgt cacggttgac atcgaaagcg atttgagcga tccgttcaat
    14641 tgcttctttt gtaaacgtaa ctgacacatt ttctgtaccg atcaacgcga cgtattgttt
    14701 tactaaagca ttatttggtt cggtcagaat cttcacaaaa tcttcagctg tcaaatcatc
    14761 taattctacc cgtatcggga agcgtccttg aagttctgga attagatcac ttggtttaga
    14821 caaatggaaa gctcctgaag caatgaatag gacgtgatct gtttggatga tcccgtattt
    14881 tgtgtttact tgtgaacctt cgacaattgg caatatgtct ctttggacgc cttctctgga
    14941 tacttctcca gattgctgtg atttagaagt gatcttatcg atttcatcga taaaaataat
    15001 tccactttct tccgctaatt tgattgcctc actatggata tcagcatcct tgactaattt
    15061 tgctgattct tccttgatca ataactctaa agcttctttg actgttaccg tccgttccac
    15121 ttttttcttc ggtgtcaaag cacctagagt ttcatttagg tcgatcccca tttgctccaa
    15181 tccattgttc atcattggtg caggtttctt ttggtcttca acttcaatgg ttacttcacg
    15241 actatccagt aaaccctttt gtagttgttc caagactgta cggcgattga ttcgaatatc
    15301 ttctgtcact tcttcttgcg gttcagcggt attttgctga ctagcattaa aaagattcat
    15361 catttgttca aattgattag tggattgttt ctgttctttt ttgataccag gtaccaagat
    15421 ttttaccaaa cgtttttctg catttttttt agcacttgtg tatacacggc tgtactgctc
    15481 tttttctacg atttggatcg cattttctac taaatcccga accattgatt ctacatctct
    15541 tccgacataa ccgacttcag taaattttgt tgcttcgact ttgacaaatg gtgcattgac
    15601 gatttttgcc aaacgtcgtg cgatttctgt tttaccgacc cctgtaggtc cgatcatcag
    15661 catatttttt ggtgtaatat cttgttgcat tttttcatct aactgcaaac ggcgatagcg
    15721 gttacgcaaa gcaacggcaa cagatttttt tgctgcgtgc tgtcctacga tatattcatc
    15781 taattcactt acgatttgtc ttggtgtttg ttttaattca tacatatcta ctcacctctt
    15841 acagttcttc tacgataata ttatgattag taaagataca gatatctgcg gcaatattta
    15901 acgcattttc tgcaatttct ttcgcactca tgtcgttttg agcatagttt ttcattgctc
    15961 gggctgcagc caacgcaaaa ttccctcctg atccgatagc taagatacca tcatctggtg
    16021 tgataacttc tcctgtacct gaaacaagaa gcatttcttt atcattcatt acgatcagca
    16081 tcgcttcaag tttttgcata gattgctgtg tccgccattc ttgagctaat tcgacagctg
    16141 cgcgttgtag attcccgtta tattcgttta gtttgccttc aaatttttct tctaaagtaa
    16201 acgcatctgc aacacttccg gcgaaaccta caacaacttc tccattgtag atacgtcgta
    16261 ctttctttgc agtgcctttc atgacaacag attcacccat tgttacttgg ccatctccag
    16321 ccatagcaaa tttcccgtct ttttcgacgg cacagatagt ggttgaatga aatggtgatt
    16381 cgaccatgtt tgtttcctcc taaatttgtt cttttcctta tttttacgct cttggatgga
    16441 aagaacgata gtttttttgt aaactttctt ttgttacatg ggcatagatc tgggtagtcg
    16501 ataaattggc atgtcctagc aattcttgga ctgtccgcaa atctgcccca ttatttaata
    16561 aatgagtagc aaatgtatgg cgcagcatgt gaggatggat atcactatcc aaactgcttt
    16621 ttttgatcat ttgattcaag acatattcaa ttccagtagg cgtgattggt tttccatgat
    16681 gattcacaaa aacgatatca tgctcttcgt gatattttgt catcaatacc tggcggcctt
    16741 ccctgaaata ttcttgcaga gcatcttgag caaaagaacc tagaggagca tatctctctt
    16801 tgttcccttt accatggatc aacatgacct ctgaatcaaa atcaatgtct gataccgaca
    16861 aactgctaca ctcacttaaa cggatgcctg acccgtaaag gacttccagt aaagcgcggt
    16921 tcctcaaatc caaaggacta tctcctgcta cgctgtcaaa aagtgcttgc atttcatttt
    16981 cataaaaaaa acgtggcagt ctcaaatttt tctttttgag atgaacgtaa gaaaatggat
    17041 tttcttttat cacttcttct ttgagaagat actgataaaa cgaacgtagg ctggctatct
    17101 tgcgactgat agaatttctg ctatagctag gacgatgatc agctaatgca ctcaaataga
    17161 cacgaaccgt aaaatattcc gccttcagca gatcagcgtc tccagattcc tccaaaaaat
    17221 gtttgaaatg gagaatgtct tcttcatatg cttctttcgt tttttctgaa tatcctcttt
    17281 cgacaatcaa ataacgcaaa aatttttgtt cccacgtggc ttctgtcatt ttctcacctt
    17341 ccttttgtcc cagagaataa tttagcacag cgtttataaa aagacaaata aattgtcaga
    17401 atttaaccaa tatttctaag tttgttcaca attggcgcaa ttcctcacat tttgtttgag
    17461 aaattcttaa gaaaaacgca agaaaaagag cgagactatc cgtctcaccc tcttcatgga
    17521 gtttccttac tcatttggga ttgcagtttt ttctaattct gtttttattg cctcattagc
    17581 ttttaaagct cgttgtgcaa ttgcttcata tcttgctttt ttgtctcgaa tgcgctctgg
    17641 cagttcagga aacagaccat agttggcatt catcggttgg aaatgttttc cttccgcatg
    17701 agtaatgtag taagccatac ttccaatagc tgtttcttgt ggaaactcga ctggttccat
    17761 ttcttttgct aagcgtgcgg cgttgattcc agcaagtagt ccgctcgctg cactttcaac
    17821 atagccttct acaccagtca tctgacctgc aaagaacaga tcttcgcgtt tcgttgattg
    17881 gtaagtcggt ttcagcaatt ctggcgaatt cataaagctg tttcggtgca tcacaccata
    17941 gcgaacgaat tcagcatttt ctaacccagg aatcatccgg aaaacacgtt tttgctcgcc
    18001 ccatttcaag tgagtctgaa agcccacgat gttgtataaa gaagctgcag cattgtcttg
    18061 acgcagctgg ataacagcat aaggacgttt gccagtttta ggatcttcca atccaactgg
    18121 tttcataggt ccgaaaagca tcgttttgaa tcctcgctgt gccattactt caattggcat
    18181 acatccttca aaatattttt ctttctcaaa agatttaagt tctgccactt cggcattgat
    18241 tagtgcctca tggaaagctt tgaattcctc ttctgtcatc ggacagttca agtaagctgc
    18301 ttctcctttg tcatagcggg acttcaaata tactttatcc atatcgatcg tagctttgtc
    18361 tacgattggt gcagctgcat catagaaata aaaaccatct gaaccattga atgctttgat
    18421 gctttctgct aatggatgag aagtcaaagg tcctgttgct actacggtga ttccttccgg
    18481 tatttcagta atttcttcgt ttatcactgt tactaatgga tgctctttga cttttcgggt
    18541 aattgtttca gaaaaagaat cgcggtcaac agctaaggca ccaccagctg gcactgctgt
    18601 ttcatctgcc gaagtgatta ctaccgaatt caatcggcgc atttcttctt tcaataaccc
    18661 tactgcatta gccaaactgt ttcctcttaa tgaattggaa catactagtt cagcaaaatt
    18721 acttgtgtga tgcgcctcag tcgatttgac tggacgcatt tcatataaac ggacagggac
    18781 tcctgcttgt gcgatctgcc aagcagcttc acttccggca agccctgctc caacaacatt
    18841 taccattttc aatgaaagaa cctcttttct attatttttg gatgtcttct tcgtaatctc
    18901 catttacaca gactacttgt ttaccgcctt tgactttctt ctcgaccaga tatccgtcac
    18961 attttggaca atttctgccc actggtttat cccaagaagt gaattcacat tctgggaaac
    19021 gggaacaacc atagaatagt ctgtttttct tagattttcg ttcaatgact tgtccttcat
    19081 gacatactgg gcaagtcaca ccgatttctt ttacgattgg tttcgtattc cgacaatcag
    19141 gaaaattact gcaagcataa aacttcccat atttccctaa ttttatcacc atcggatgtc
    19201 cacagacatc acaatcgaat cctgctggtt catctttgat ttgaattttt tcgatttttt
    19261 cttccgcatt cgtcagttct ttttcaaacg gtttatagaa acgatcaacc actttgaccc
    19321 acgcttccgt gccttcttca attttatcca aatctccttc catttctgct gtgaaatgga
    19381 tatcgacgat ttgagggaag aattcacaga ttaatgaatt gacaatttct cctagttcag
    19441 tcggctcaaa tcttttttgt gtcaatttta catagtagcg tctttggatc gtatcaagtg
    19501 ttggtgcata agtggaaggt cgccctaccc cgttttcttc tagtgcacga atcaacgtag
    19561 cctcactaaa acgtgctggt ggttgagtaa aatgttgttt tggttcaata tcaacagatt
    19621 ggactacatc gccttcttca agttctggaa gaatattttc tttctcttct ttgccgtcat
    19681 ctctaccttc cacatacact tgcatgaatc ctttgaattt gatttttgaa ccgttcgcaa
    19741 tgaagatgac accattttgt tctaatgtca ctttcattgt atctaaaata gctggtgtca
    19801 tctgactagc cacaaaacgt gaccagatca atgtataaag tttcaattga tctttatcta
    19861 aatattgttt gagttcatct ggtgtacgaa gcacactcgt tggacgcaca gcttcatggg
    19921 catcttgtgc gccttgagca tttttgatct ttcggtgatc atgtctagaa aattcttttc
    19981 catacgtatc ttcaataaat gctgcggcct ctgcttttgc tgtatcggca atccgggtag
    20041 aatcagtacg catgtatgta atcaagccga ctgttccttg tttacccaaa gtgattcctt
    20101 catataattg ctgtgctacc atcatggttt ttcttgttcg aaagtttaac ttacgggcag
    20161 cttcttgttg caggctactt gtagtgaaag gattcgcagg gtttcttttg cgctctttct
    20221 tttctacttt tttaacttcg aagtctgttc catcaattct agcagtgact tcttttacac
    20281 tttgtgcatc tggtaatttc tttttcttac cgtcgattcc ccagaaattt gctttgaatt
    20341 tcttgcgagc ttttttaaaa ttaccgtcga tgctccaata ttcttctgga acaaattctc
    20401 ggatctcttt ttcacggtca ataataattt ttaaagcgac agattggaca cgtcctgcac
    20461 ttaacccttt cttgacctta cgccagagaa taggactgat cgaataacca acgattctgt
    20521 ctaaggtacg acgagcttgc tgtgcatcta ctaaatctac atcgatcgtt cttggttcct
    20581 taaaagctgc tttgactgcg tcttttgtga tttcattgaa gaccacccga tttttttctt
    20641 tcagatccaa cccaagtagg tacgctaaat gccaagcaat cgcttctcct tctctatccg
    20701 ggtcacttgc caagtaaact ttttgagctt tttttgctgc ggctttcagg cttttgatga
    20761 catcgccttt tccgcgtata gaaatataat ggggttcata attgttttca aaatcgatac
    20821 ccattttact tttcggtaaa tctcttatat ggccaacact ggccattact ttatagtttc
    20881 gtccaagata tttctcaatt gtcttggctt ttgcaggtga ttctacaatc actaaatatt
    20941 tatacgccat tactgaatgg actcctttcc attgtcgtgt tcctactatt aaaagaggat
    21001 tcatacaaat cgtagctaat catatccatt cagaaaacgt ttgtcaagaa aagcaaatga
    21061 aaagtatggt gttattcttt aaattctgcc aagatatctt cacccgaaac cgtgcaaact
    21121 gcaccgtctt ggatcaaatg atgacatccg gtagaatgtt ctgttaaaat atttcctgga
    21181 acagaaaaaa ctgttcttcc aagatccaat gcttgctgtg ctgttatcag agagccgctt
    21241 tttttcttgg cttcaaagac acatgttcct ttagacaaag ctgcgataat ccgatttctc
    21301 attggaaaac aatgcttttt cgggccggat tgaggaggat attcactcaa aagaagatga
    21361 tcatttgcaa tcacctcttg caagtcacga ttttcagcag gatagacttg ctctatccct
    21421 gttccaatga ctgcgattgt ccgtccacca tgagagattg ctgcttggtg accgtatgta
    21481 tctatccctt tcgcgcagcc actgacgatc acatatccag cttctatcac tggttttagt
    21541 aaataataaa ctgttttttt ggctaaagca gaagtgtgtc ttccaccaac aacgcttaac
    21601 atgttacaag aaagtaaaga tagatcacca ttataaaaca gtacaattgg cggataggtc
    21661 aagtgaagca gctgaggggg atatttggga tcaaagcaag tgataaacga ctgtttcgat
    21721 tggattttgc cccagttagc gtgaatttcc tgccaacttt tgcgaaacag ctcccggtgt
    21781 ttggttattt tacctatctt aatgatgtct tcaaatccga tttctttata aggatactgc
    21841 atcatccagt tagcaagtgc ccattttcct tgactactga ttccttttgt ataagcaagt
    21901 ttcaataaat tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat
    21961 aagatacgta aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt
    22021 acgatggatt ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata
    22081 acctgcatta tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg
    22141 gtctcgatat actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc
    22201 tttgattatt ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata
    22261 gcttggtaca atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata
    22321 aatattcaca tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt
    22381 ttcttgaact tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt
    22441 cagccctaat atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc
    22501 taatggtcct cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc
    22561 ttgcttttca aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa
    22621 tcgtcgttca aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga
    22681 ttctagtgta ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat
    22741 ggattcagtc atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa
    22801 gttttccttg acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat
    22861 acccacgttt tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga
    22921 ttgataaacc ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc
    22981 cgtaaatcgc taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta
    23041 gttttttgcc gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca
    23101 attccaactc attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc
    23161 ctgtctgtgc aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg
    23221 gaatacctaa gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg
    23281 cccgtttttc tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt
    23341 cttgtgccgt gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct
    23401 ttgtctgttg tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct
    23461 gaatgatctg atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt
    23521 caaatacgat gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat
    23581 gtcccggaaa ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc
    23641 cggctttact tgtttttttc tttttcaata taaattcact tttttattat aaagaaattc
    23701 catctattag ttaagtgaaa gtgaaccagt cctcattcta tcacatcttc acttttttga
    23761 tgatgcaaat ttttattttg gtttctatat atgaatctaa atttttgaca gcgtttccta
    23821 aataagagat gatagacgaa caaaacagaa aggagacgaa acaaatgaat agtatggttt
    23881 ttgttaattt tcctgttgct gatgtcaatc gttccgttgc tttttatgaa aagctgggtt
    23941 ttaaaaaaaa tgaagaattt tctactgaa
//
LOCUS       DANKAY010000035        23584 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000035 DANKAY010000000
VERSION     DANKAY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="PWY98_001384"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="PWY98_001384"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2037143.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="PWY98_001385"
     CDS             complement(1171..1866)
                     /locus_tag="PWY98_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2037144.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="PWY98_001386"
     CDS             complement(1879..2595)
                     /locus_tag="PWY98_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2037145.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="PWY98_001387"
     CDS             2925..3533
                     /locus_tag="PWY98_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2037146.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="PWY98_001388"
     CDS             3858..4181
                     /locus_tag="PWY98_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2037147.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="PWY98_001389"
     CDS             4363..5733
                     /locus_tag="PWY98_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2037148.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="PWY98_001390"
     CDS             complement(5863..7164)
                     /locus_tag="PWY98_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2037149.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9476)
                     /locus_tag="PWY98_001391"
     CDS             complement(7518..9476)
                     /locus_tag="PWY98_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2037150.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="PWY98_001392"
     CDS             complement(9653..9919)
                     /locus_tag="PWY98_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2037151.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="PWY98_001393"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="PWY98_001393"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2037152.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="PWY98_001394"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="PWY98_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2037153.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /locus_tag="PWY98_001395"
     CDS             13263..13826
                     /locus_tag="PWY98_001395"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2037154.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="PWY98_001396"
     CDS             complement(14079..14600)
                     /locus_tag="PWY98_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2037155.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="PWY98_001397"
     CDS             complement(14603..15445)
                     /locus_tag="PWY98_001397"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2037156.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="PWY98_001398"
     CDS             complement(15438..16163)
                     /locus_tag="PWY98_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2037157.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="PWY98_001399"
     CDS             16339..16635
                     /locus_tag="PWY98_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037158.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17203..18795
                     /locus_tag="PWY98_001400"
     CDS             17203..18795
                     /locus_tag="PWY98_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2037159.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="PWY98_001401"
     CDS             18808..19548
                     /locus_tag="PWY98_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2037160.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="PWY98_001402"
     CDS             19716..21065
                     /locus_tag="PWY98_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2037161.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="PWY98_001403"
     CDS             complement(21193..21783)
                     /locus_tag="PWY98_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2037162.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="PWY98_001404"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="PWY98_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2037163.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6743 a         4757 c         4497 g         7587 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 attt
//
LOCUS       DANKAY010000036        23435 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000036 DANKAY010000000
VERSION     DANKAY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            332..745
                     /locus_tag="PWY98_001405"
     CDS             332..745
                     /locus_tag="PWY98_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2037164.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1365..1529
                     /locus_tag="PWY98_001406"
     CDS             1365..1529
                     /locus_tag="PWY98_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037165.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            1526..1756
                     /locus_tag="PWY98_001407"
     CDS             1526..1756
                     /locus_tag="PWY98_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037166.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            1941..2486
                     /locus_tag="PWY98_001408"
     CDS             1941..2486
                     /locus_tag="PWY98_001408"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037167.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2487..2804
                     /locus_tag="PWY98_001409"
     CDS             2487..2804
                     /locus_tag="PWY98_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2037168.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2961..3125)
                     /locus_tag="PWY98_001410"
     CDS             complement(2961..3125)
                     /locus_tag="PWY98_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HDL2037169.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            3227..3748
                     /locus_tag="PWY98_001411"
     CDS             3227..3748
                     /locus_tag="PWY98_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2037170.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            3735..4208
                     /locus_tag="PWY98_001412"
     CDS             3735..4208
                     /locus_tag="PWY98_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2037171.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(4215..4373)
                     /locus_tag="PWY98_001413"
     CDS             complement(4215..4373)
                     /locus_tag="PWY98_001413"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HDL2037172.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4466..5227
                     /locus_tag="PWY98_001414"
     CDS             4466..5227
                     /locus_tag="PWY98_001414"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2037173.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5447..6766
                     /locus_tag="PWY98_001415"
     CDS             5447..6766
                     /locus_tag="PWY98_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2037174.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            6782..8002
                     /locus_tag="PWY98_001416"
     CDS             6782..8002
                     /locus_tag="PWY98_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2037175.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            8037..8714
                     /locus_tag="PWY98_001417"
     CDS             8037..8714
                     /locus_tag="PWY98_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2037176.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            8711..9904
                     /locus_tag="PWY98_001418"
     CDS             8711..9904
                     /locus_tag="PWY98_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2037177.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            9919..10245
                     /locus_tag="PWY98_001419"
     CDS             9919..10245
                     /locus_tag="PWY98_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2037178.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            10249..10584
                     /locus_tag="PWY98_001420"
     CDS             10249..10584
                     /locus_tag="PWY98_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2037179.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            10581..10976
                     /locus_tag="PWY98_001421"
     CDS             10581..10976
                     /locus_tag="PWY98_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037180.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10973..11344
                     /locus_tag="PWY98_001422"
     CDS             10973..11344
                     /locus_tag="PWY98_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037181.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11360..11920
                     /locus_tag="PWY98_001423"
     CDS             11360..11920
                     /locus_tag="PWY98_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2037182.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            12045..12356
                     /locus_tag="PWY98_001424"
     CDS             12045..12356
                     /locus_tag="PWY98_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037183.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            12380..12571
                     /locus_tag="PWY98_001425"
     CDS             12380..12571
                     /locus_tag="PWY98_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037184.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            12571..16419
                     /locus_tag="PWY98_001426"
     CDS             12571..16419
                     /locus_tag="PWY98_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2037185.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            16420..17292
                     /locus_tag="PWY98_001427"
     CDS             16420..17292
                     /locus_tag="PWY98_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL2037186.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            17289..18338
                     /locus_tag="PWY98_001428"
     CDS             17289..18338
                     /locus_tag="PWY98_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037187.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            18342..19166
                     /locus_tag="PWY98_001429"
     CDS             18342..19166
                     /locus_tag="PWY98_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037188.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            19184..21244
                     /locus_tag="PWY98_001430"
     CDS             19184..21244
                     /locus_tag="PWY98_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL2037189.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            21261..21410
                     /locus_tag="PWY98_001431"
     CDS             21261..21410
                     /locus_tag="PWY98_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037190.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            21407..21853
                     /locus_tag="PWY98_001432"
     CDS             21407..21853
                     /locus_tag="PWY98_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037191.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21855..21992
                     /locus_tag="PWY98_001433"
     CDS             21855..21992
                     /locus_tag="PWY98_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2037192.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            22027..22269
                     /locus_tag="PWY98_001434"
     CDS             22027..22269
                     /locus_tag="PWY98_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDL2037193.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22285..22482
                     /locus_tag="PWY98_001435"
     CDS             22285..22482
                     /locus_tag="PWY98_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2037194.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22493..>23435
                     /locus_tag="PWY98_001436"
     CDS             22493..>23435
                     /locus_tag="PWY98_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2037195.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATND"
BASE COUNT         8128 a         3989 c         5199 g         6119 t
ORIGIN      
        1 aaaaaatact ataggtaaaa aagataacat gaggagctta tttgttctag agtgtttgac
       61 aaagtaacaa aatgcgacga taccaataca aacgggaatc caaaataaca gcatgctcca
      121 catagtcact caaccatctc ctatacgctt tattatcaat agataatatc atttttcgta
      181 ataaagataa aaaaataagt aaggcttctc attaaaatat aaaaaagaca gccgaccact
      241 gactgcctat ataagagtat tgaaataaaa agctgctgat ataataaatt ccacaagttt
      301 attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg tagatttcaa
      361 acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg agcggatggc
      421 aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga aggcgccgaa
      481 gcacggcaat aaggcagaag acgcaatcat tcagatgatg gatatagaag cggagagaga
      541 cgcgattcta gcagccttga tggctcttag tctgattagt cgtcagatac tctactatag
      601 cttttgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg gttattcaga
      661 aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag catataaaca
      721 cggaagaata attgcttata aataatttgg cggctttttg gcggaatgat ggcggttttt
      781 agctatttac cagtgatatt atggtagtgt cgaaagatta gtgataggtc taagacaaaa
      841 taataataaa aggaacatcg ttttattatt gtttcacaat taagcttcga tagacagcaa
      901 cggaaatatt aagaataagg atgtgaattt taactccttc taaattgttc ttattatcta
      961 tcatccgttg ctgtctattg tttttttaat tattcacacg ataactaaag gtgagcgaag
     1021 agaaatgatt ccattaataa tttcaatttt tgcgctttgt cttaatgtct atatgattgg
     1081 atttaaaaat gggcaaaata aaagatagta gtaactaaga aagaaatttt tatattgtca
     1141 ctgtggcgga aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaactatgc
     1201 aatgttcgat tcattgccag tgactttggc aaccgaggca tcggcggttt aaaaatatag
     1261 gggtgcgcaa tttcgtacgc gttttgtgca tcgtgtaggt tgctattaca tattagatca
     1321 ctcgttgagt ggtcttttta ttttacataa aggaggtaac aacgatgtat agaccacaat
     1381 acttagaaca gaagtatgaa gtaatcactg tgcaaaacgg taacggtgag atagtacgaa
     1441 agtatagaag accaataaag agcgatacat ataaacgaaa ggaaaacaat gaagttattt
     1501 cttttcgcag aaggagaaaa gccaaatgag aaactactgg tatgtatcgc taactaatga
     1561 atatcctcga accattgatg attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg
     1621 gaagtactct attgtcgaaa tgctaagaga agctacacca aacgaagtgg ataaatgcaa
     1681 gctgatatat tgcggtcatg gctattggaa agacgagtat atccaacaaa acattgagag
     1741 gtggatagat agatgagtta cctcgaacat ttgaaacgtt gctacatgca ttctaagaat
     1801 aaacttcctg acagctacac tgtagatgat gttgctatac atgtattgaa gactgagagc
     1861 catagcagtc ctgatggtag cagtaaggag cagacgctcg cgtggttcaa gttctttaag
     1921 tggattaagg aggaagagta atgcacatgc acataggaga aaagatatat aaattgagac
     1981 ggcttaataa cgaaacaatg aacgacttag ttaaacaaac tggagtgtca aaatcatggt
     2041 tatctgatat tgagtctgga aaaaagcaac gcgttgattt taataagata tgtgcaatag
     2101 ccaaacatta taaagttagc ttagaatact ttcgggatga ttatttggat agtgatgaac
     2161 acgaggatat tattcctttg acaggcgaaa caagagttaa atcagtgaaa tcgcttgaac
     2221 aaagcctaaa gtatctgaat aacaaacaag aacctaacct tctagagatc aaactaaaag
     2281 atactgactc agtaccagag gtttggtaca agggtgagag gttggatgaa ttgcctaaag
     2341 gattagtaga tgtctcgtat cattggaaga ctgatgattt tactaatgat gatagaggag
     2401 cgaacgacat cacgattcaa tacttttcta gctttaatga taagtatcca gataagaaaa
     2461 caatcggaca caagagagat atgtaaatga aagaagttag acctagagac gaaatagaca
     2521 aactatacaa gaccaaacga tggcgagacc taaggcaagt agtaatagct agggacttcg
     2581 gcatgtgcca agagtgcaag cgtcgagggc ggaacacaag gggcacgatc atccatcaca
     2641 tagtcgaggc gagggaagac ctgtcactgt tctggtccgt agataacctt gaatgtatct
     2701 gtgtagcttg tcacaacaga gagcatccag agagatcagg cgggaagaag aaaccaaaac
     2761 ctaaatcaca tatcgttaaa atgtattcaa ctcctgaaag ataagtttgc agcgaaatga
     2821 aggtagcccc cctactctaa aagattaaag agtaaggctt gagaagaacg gtgctgtcct
     2881 tccttcgtaa aaagaccgct tttcaagttt tttggagaaa aaggaaaaag ccgaccaatt
     2941 taagccggct ttggacgaag ctatttctta gtccatttgt ttcctttttg agaagtaggg
     3001 ggtaatcggt cgcctggatc aatttttact tcttgtccgc cttggacatt tccaccacga
     3061 ggacccactt ctttgtaggt tccttttggt ttgttgtctt cgccgggttt atagagttct
     3121 cccataggaa tccctcctta acaaatttcg gcacagcact gccgataacc taattataag
     3181 gattgtgata acgatttcaa tctatctttt gaaagaaggt gatattatgc cgcaaccagc
     3241 gaagagtgca aaattacaat tattaaacgg aaacccaaat aagaagaata ccgaagaact
     3301 ccgcaagcga gcggccgcag aagacaaatt aaaaatggct actgacaaaa tcaaaccgcc
     3361 gttatggcta gattcgctag gaaaggatac ctttgagttt atcgccgatg aattgctgtc
     3421 tgtggattta atcagtaatc cggacgtcca tacaatggct ctatactcca attggtattc
     3481 gcaatacgtt tctttagaaa aacagcttcg aaaactacaa cgagagtaca agttgaacta
     3541 tgcgcttgcg aaaaaggagg cagaggcgag aggtgagccg tttaatgaac ctaatgaatt
     3601 aattggtaat ccgctctctc ggcagatgga tacagcgtct cggaatctcc gttcttttgg
     3661 cgctgattta ggactatcac cagcagccag agctaagtta gctattaaga tggctgatga
     3721 tggtggtgat gacgatgacg acttctaata ttttggatat gtcttacaca gaacgtgtgg
     3781 actattggca aagctatctt gaggagcaag cttcttgggg tggcttttta aaatgtccat
     3841 atccggaatt gttaactact tggtatgcgg aacgattaat cgatggaagc ataccagcca
     3901 gcaaagaaaa tattcaagct gctaaacggc atatgcgtga tttgcagcgc caaggaacag
     3961 atgattttcc ttggatcttt gacgaagaaa aaggtcaccg gcctattaga tatatcgaaa
     4021 aaaaatgtaa accaactgaa ggcgactttg gttcgtttgt tttgcaacct tggcagcatt
     4081 tcataattgg atccatgtac ggatgggtac atcgtgatac aggagagcgt cgcttccgcg
     4141 aggctcttat ttttgttgga cgtaaaaacg gtaagttttg ccgtttaaaa atcgggcaaa
     4201 atcggtgaaa acctttattt ttggctttct tttagtaata attcgatagc tttatcaagc
     4261 aatttagaca taggaaccat agtttcttcc gacatttttt ttaatccttc gtataaatct
     4321 ttatcaattg cgttagaaat tctaattctg ttttttagtc cgtatttgtc catgtgtgac
     4381 actccttttt attttactat aacatccgat tccaccgctt gcaactacca tcaattgatg
     4441 gtagtatata aaagaggtga gtagattgaa aaaatacatt atttacaaac acttaaataa
     4501 aataaatggc aaaatttata tcggtgtcac aaatgatatt ggcagaaggt ggaggagcgg
     4561 tgggattgaa tataaaccac caaagaacga aacccaacac cacagaagtt tttggaatgc
     4621 aatacaaaaa tacgggtggg aaaattttga tcatttgatt attgaagaag acttaacgat
     4681 gaaagaagct tttgagaaag aaaagtttta tattgaactt tatgactcaa ctaataagaa
     4741 aaaaggttat aacatcgcaa aaggcggaaa tggcggaata atatataaag ttcaccccaa
     4801 aggaatgcta gggaaaaaac aatctaaaga attttcttca aatcacagta aatgggcaaa
     4861 gaaccataag aataattgta tgacaaacgg tgatgttgta tggggtgtta ctcacgaaca
     4921 cccaaaaggc atgttgggaa agcaccacag caaagaatca attatgaaaa agaaagcata
     4981 tagtggtgaa aatgctgtaa cctcaaaacc aattgtcgca attgaacggg atggaactaa
     5041 aagagaattt cacagcgcta agctttgcat ggcttactat tcgattagta catgtgtttt
     5101 ttaccgactt ttaaaagatg gcgcccctta tgtgataaat cctaaagcga actacagaaa
     5161 taaagaaaaa atattagcta tcgaaggaat tatgttcaaa cataaagaag ataccgaggt
     5221 aagttagtag attgcgaaag gttactaact accgtaacgc gtaggagttg aataaatata
     5281 atgctcccaa gagtgtccga cagcagataa tttgctgaaa atgtacgcta aactgggcca
     5341 gaactgactg accgatgaaa atgagggtga cctccagagt gcgagataaa aagctcgcag
     5401 ataataacaa tcgaaaacat cactaatttc aggcctttcc acatacatgg tcgcttatga
     5461 tgatgaacaa ggcgccaacg tttacgtatt ggcaaatgct cgtgatcaag caagcttgtt
     5521 gtttgataag gccgcagaaa tggtcaaaca atcgccggcg ctctttaaga aatttggtaa
     5581 gcctaaacga tcaagtatta attatgctcc cgccttttct aaaatggaac cacgcgcctc
     5641 agatagccgg aaattggatg ggctaaacac tcactttggt atttttgacg agatccacga
     5701 gtttacgaat tacaatctga tcaacgttat caagaaatca agaggaacca gaaaacagcc
     5761 tctgatagtt tatatcacaa ctgctggata tgtattagat ggtccgttga tgtcttattt
     5821 tgagcaaggt gtggactgtt tggaacattt ggaagatgac atcgatgaac ggactttcta
     5881 ttatctggca aaacttgaca gtgcggaaga ggctgatgac ccaagattat ggatcaaagc
     5941 caatccgaat atttgtctaa tgaattttgt tggcatgcta gatgactatg ttaaggataa
     6001 aaaagatcca aaagaatatg ctgactggat taccaagcaa tttaacttgt tttccgatat
     6061 cgatgagctg tcatttgtcg atatgcctac cattaaacga aacaataaaa ccatcgatat
     6121 tgaaacgctc aaaggtaaga agtgtgtcgg tggttttgac ttgtccgaaa cggaagactt
     6181 taccgcagcc gttttagaat ttccgcttga aacaggcgag gtattcattt tgcaacacac
     6241 atggatccca caagctagat ttgatcgaga taacaatcaa gagcgtatca aagcgtggga
     6301 gaaggtggga gatctaacga ttattcctgg tgattacgtc aattatgaat acgtcttaaa
     6361 ttggtttgta gaaaattcga aaatctatga cattgtaaaa atcaattatg acaaggccaa
     6421 ggcgctacga ttaaataaag aactagaaaa tgcaggattt gaaaccaacg agattcggca
     6481 agggtttcta tcattaggtg ggccaatgca aaacttcaag gaaatgctat tggacggtaa
     6541 ggtgattttc aacaattcca agctttaccg atggtatcta tccaacgtca agctggtgat
     6601 ggatcgcaac tcaaactgga tgccgtctaa gcagtccaag agtagaaaaa tagatggttt
     6661 tgcagcaagt ttgaacagcc acgccgaagt gttgaatatg ttggttaatc ctgtcggaac
     6721 cgggaaagta acctattact cgatttccga tttaatgaat atgtaagaaa ggtgtggagg
     6781 aatgagtatt ttagatcgtt tgcgttcttt tggccgagcg aagccgaaag cgagcaaaca
     6841 agagtatttt ttgaatgacc cgggattgat accgtattta gtcggaaaag atgaaatatc
     6901 agaagggatt ttttccgtaa ttagccgtgt atcgaacgtt tttgcgtctc tccctctcaa
     6961 aatgatagat gtggagtttg gccaaccgga cgactgtcct gcatacaact tgttgagcga
     7021 aggccctcga tattttacaa agtttgattt tttccgggac gtggaagttt tgagaaacta
     7081 ccaagggaat gcgtatgtgc agattttccg aaatatcaat ggagaagtag cagatatggc
     7141 gttagtaaaa cctggtgctt gccatccagt gattgatatg gatagcgggg agctttacta
     7201 ccaagtaact gcgactgaca aaggcagtta caagcaagtt atctatgtac attacatgga
     7261 aatgcttcac tttaaacaac cgaggtttgg cggcttggaa ggtacagacc ccacaaaagt
     7321 attaacgaat accctcggat atgatcgaga agtccgaaaa atctctttaa gtcagcttaa
     7381 aggaagtaat gaagggctaa aagttaagtt tgctagcaat atggatgaag aagctaaaaa
     7441 agctacagtt aaaaacattg ctgattttta tcgacaaaac ggtggactac ttgtggaaga
     7501 aaacggtgta gaaatcgaac gtttacaacg agagctggta gacagcaagc ttttagatac
     7561 tgataaagta tctcgctcca gaatcgctat ggtctacaat gtgccggagc atttcatcgg
     7621 gaataaccag tcgagttact cctctcagga acagctcaat atggaatttt tgacgtacaa
     7681 tctagtaccg accgttaatc aatatgaagc ggaactaaat aagaaaacac tatcgagaac
     7741 cgaaaaagca aaaggttatc gatataagtt taatgtcgca agtttgctaa gagctgacac
     7801 tcaggcaagg ggacaattct atcaaatcat gcgacgaggc ggagcatact cagcgaatga
     7861 tgtccgaaga tttgaggact tgcaaccgat aaataaaacc ggtatggatg attaccatat
     7921 ttccggagac ctatatccaa tcgatatgga tccaacatta agaaaaacaa cctcgtctaa
     7981 aagcgtagcc gaaaacggtt aggttttttt agtttgcacc gaagggaggt ggaaaaatga
     8041 aaaaagtgac gttaagcggc gatgtcgtgg ataacgatac cgcgtggctt tatgactggt
     8101 ttgggatcaa ttgtatctca ccagggaaaa tttctgccgc tcttacagaa gcagcggggg
     8161 atgaagtaga acttgatatc tcatcgaacg gtggggatgt cctagcggca agcgaaatat
     8221 ataccgctat ccgtgcctat ccgggaaagg tatccggaaa tgttgtgagt attgcagcaa
     8281 gcgctgcaag tgtaatcgct tgcgcttgcg aaccgcttag aatctcacct acggcacaca
     8341 tcatgattca taacgcatgg gtgaccacta gtggcaacgc tgaggaatta aaagccaatg
     8401 cagaaatgtt aagcagtgtg gatgagtcta ttgttaatgc ttacgagatc aaaacaggac
     8461 tcgatcggaa aaaacttgct gatttaatgg cgaaagatac ttggttaaat gctcaaacag
     8521 cagtagcaga agggtttgca gatgaaatta tgtttgcaga agcaccagta acggtactca
     8581 atgcctctca accggttatt ccaaaaaacg cagtaactaa gttgaaaaat ttaatactca
     8641 aagcggaagc accgcaagaa gaaacactct tacagaaaaa actaaaagcc ttaaatggag
     8701 ggaaaaacga atgaatttag aacaattaaa aaatgcgtgg gtcgaggcgg gaagtaaagt
     8761 ctctgactta aatgcacaac tcaatgcagc attggttgac gatgaaaaaa cagaagaaga
     8821 tgtagtaagt ttgcaagcac aagtaaaagc agcacgggct aaacgggacg gattgaaaga
     8881 gcaagtggca aatatggaag ccgaacaagt cttaaacgtc aaaaaagaac cattagataa
     8941 aaaagatgaa aacttgaaaa acaagtttat caaagacttt aaagcgatgg tcaatggtga
     9001 tcctgctatt atggctactt tgacatctaa tacggatgaa tctggtaatg ctatcggatt
     9061 gactattcct gtagatgtgc aaacgactat tcatactttg gttcgtcggt ttgactcttt
     9121 gcaagaatac gtaaacgttg aaaaagtgac cactaccagc ggttctcgcg tttatgaaaa
     9181 atggtctgat attacaccgt tgactgcttt ggatactgaa gacggtgaaa tcccagcaaa
     9241 tgatgatcct gcactttact tgatcaaata cttgatcaaa cgctatgcag gtatttccac
     9301 agtaactaac agcttgctaa aagatactgc cgaaaacatt ttggcatggt tgtctaaatg
     9361 gatcgcgaaa aaagtagttg ttactcgcaa tacaaaaatc ttggcagcta ttgatggaat
     9421 caaagcggca caaaagaaag atgttacaga tgttgatgga attaaagata tcgtaaacgt
     9481 ccaacttgat ccagctatcg aagctacatc tatgtttatt acaaaccaag atggcttcaa
     9541 tgttttagat aaagtgaaac gtgctgatgg atcttacttg ttacaaaaag acgtagcttc
     9601 tgcaactgga tataccttct taggtaaacc aatcaagaaa attgcttctc gtttcttgcc
     9661 aaacaaaggt acgcaagcta ctcctaaata tccactgtac attggtgatc tgaaagaagc
     9721 cgttacattg tatgatcgcg aaaacatgag cttgctgaca acgaatattg gtggtggagc
     9781 ttttgaaaca gacaccacta aagtacgcgt catcgatcgc ttcgatgtgc aactagttga
     9841 tgatgaagcg gttgttttgg ctacttttac aactattgcg aacgagacac cggcgaaagt
     9901 ttaaggagct gattccttat gattcttgat cctaaaatgg atttaggcga aatcaaaaac
     9961 gcactaaaga ttgataccga tgatgacgat gtggaagtaa gccgtgcggc acaagctgca
    10021 attgcataca ttagaggggc tatcgggaat gataagccct ctttttataa gcaagaaaat
    10081 gaaacggttg atctgattaa tttagctatc ctgcaattag cagatcatta ttacaacgct
    10141 cgatcagcaa ccgtgagtgg gaacttgcga gagtacgatt taggttttac aagcctaatc
    10201 ttgcaactca aagcaagtta tttgcttttt gtggaggagg agtagcgtat gccccttatc
    10261 caaacaggaa atttaaatca acgcatcaag tttgtccgag atacgacggt taaggatgag
    10321 gacgggcaag ttgtcccgac ttctacaacc attcttactt gctgggcaag tgtgcagaca
    10381 caacgcctga acgatattaa gacgtcgatt ggtacggctt tggaaggaac actgacgttc
    10441 attatccgct accaacaaaa atcagagctg accaatgata tgaaagtgcg ttggaatgga
    10501 aaaacgtttg aaatcattac gattacgaaa ggcgagtttg cgaaggactt cacgacaatc
    10561 attgcaaaag aggtttcaaa atgagtgtag aagtcgatgc aaccgaagtg tacaaagcgc
    10621 ttagggaagt aaaagcaaac gttcaacgag tggaaagccc agcacttaga aaagctgggg
    10681 agtacgctca agaagagtta cgacaaaaca caccttactg ggatggaacg aagtcaaacg
    10741 gtaaacgtgg ttcgtatatg caagaacatg ctaagaacca tgtggttaca agctcgataa
    10801 aaaacggatt ggtagaagtc ggctatgaca aagatgtttc ttggcggatg cactttatcg
    10861 agttcggaac aatcaaacaa cgtccaaaag gtttcgtaca aaaaacacaa aagcaaatcg
    10921 aaaaacaagt aacacaaatc attgctgacg aagtaaaaag gaggctagga ctttgaaaac
    10981 ggcagtatca caagtctatt caattctgaa tagcaatgaa aaaacaaaga acattgattt
    11041 ttacaccaat agtgttccgg aatcagctca aacagtacct agccttccag ttggcagaat
    11101 tacagagata tccggcaact atgaagattt tgcaagcaac aatcctttga ccattcaatt
    11161 taacgtacag gtagatgtat gggtgtcaac catgaaagag gttgatgcct tttattttgc
    11221 ccttgatgag gttatgaggg ggaatggttg gcaatgcgca tacacggaac aaacagatga
    11281 cgaggacttg gaaggtgcaa agcggattat caaacgatat gtagcaaata tttcactaaa
    11341 ttaaaaggag agaaaataga tggcaacagt aggattcgag agcgtcattt ttggcgtaaa
    11401 aacaggtgca ggcggcactc taaaagaatt agtagcagat aagtcgaaag gcggagcgat
    11461 cgaagctaaa attactggat taggcgcaac ttctaacaca acatacgctt caaacgtacc
    11521 gttcttcatt gcaagtaaag gggtttcgtc gccaaaagtt acgcttgacg tggcagactt
    11581 aatggataac ggcatttaca gcgaaatcat tggtgctaaa accgtggatg gtgcaaatgt
    11641 aattggttca gaaactgaag cgccttacgt gtcggtagtc atggttacag cgaacaaaga
    11701 aggaaaacgc ttgttcatgg gattgacaaa aggaaaattc agtcatccag atatcgacat
    11761 gaaaacagct gaagacaaag gggtagaatt gcaaaccgat tccatcgaag gggaattcat
    11821 ttctgatgaa cgtggctatg tatacttaac agccgtagaa tcagaaggaa tgaccttaca
    11881 aaaattcaag gacttggtaa ataacaaaga gggggagtag ttaaccctgc atctacacca
    11941 acgacagata cagggacacc aaaagaacca gaaccaaaaa ttgatacaca aggttagcca
    12001 tttttggcta gccttatttt ttgtaaaaac aaggaggaaa acaaatgatt gaattgcaat
    12061 tgaaacttga cggaaagaaa aaaacattca aacaacaaga tatttccgca cgtgcaatgc
    12121 gtgagtgtat caaattttac gagaaagcgg aaaaagcaga cctaactgat ttagaagcga
    12181 ttgattcaat gattgcaatt acagcagata ttttccaaga tccagcagtt acatttgatg
    12241 ctattttaga cggtttgact gcgagcgagt tagtaccggc attagaaagt gtttttgaac
    12301 aaataaatga actgggaaac aatgaaaaaa agcagacggc gagcaaaaag aaataagttt
    12361 ttctgaagct aggaaagcaa tggatcaaat ctacaaagat ttaatcgaag caggttggac
    12421 gatgagagat gtggacgaag ccgactatca ttatttgtta cacctttttg gagaagtgga
    12481 gagtggcgaa gaatatgtag atggtgctga tttcatcaaa caatttttat cggctgaaga
    12541 cttagtaaaa cttgaggaag gaggtaaata atggcaggaa aaggacaacc ggcaggaaat
    12601 atcaagctag ggattagttt agatagcact agttttggta acacgctgga cgaaatcaat
    12661 gcgaaagtca aacaagctga gtcgaatatg cgtgccaatc taaaggctta tgattcagca
    12721 ggacgttcat acgaagcact tagtcaaaag acgaaagact tgtctacggt tatggaaggg
    12781 caaaacgcca aagtaagaga attaacaaag cgccgtgatg aagcgattag caagtatggc
    12841 gaggaatcga aacaagttgc taaccttaac acacagataa acaatgctac cgcaaaatat
    12901 aatgcttaca gtcgccagtt gaacgacaca aaaaaagaat tggtgtattc caaaacagcc
    12961 gtcaatgatt tatctaatga aatcaaagaa aatgaacgac aaatgaacgc cgaagtaaaa
    13021 gcgttgaaag ccgctggtga tgaatctggt gcttttgaag caaaacaaaa agggctagcc
    13081 aaacaaacgg aattatccga gaaagctatc gaagaacagc gcaaagttgt gaaactgatg
    13141 gctgatgagt ttggcgattc agcaaatgaa accgaagatg caaaaagggc attagaaaag
    13201 ttagaacgac aaagccaaat atctagcagg caattagaag cactcaaaag ctccagcgat
    13261 caatcaggaa aagaaataga agattttggc gacaagtcca caaggtcagc taggaaactg
    13321 gacggactaa aagacaaatt aagctcgcta aaaagcgcat tttcgtttgg tgcagttgct
    13381 ggattagcgc ataacgctat tagcagtgta gtaagtggcg tgcaaggttt ggttggcgaa
    13441 gcagtaaacg catcggattc attgatgaag ttttccaaaa ccatggagtt tgctaacttt
    13501 gggaagtcac agatagaaag ctcgaaaaaa gaaatgaaag actacgccga taagacggtt
    13561 tatggtttag aagaaattct gaacacaacc gcacaattgg catctaatgg gattcctaac
    13621 tatacagaac taaccaaggc ggcaggtaac ttgaatgccg ttgcaggcgg ttctagtgat
    13681 acattcaaat ccgttgccat gatgctaacg cagacggcag gagctgggaa actaacaact
    13741 gaaaactgga atcaattagc agatgcgata ccgggtgctt caggattgtt acaagacgct
    13801 atgttgaaaa acggagctta tacaggaaac ttccgtgatg caatggcgca aggtcaaatc
    13861 acttccgacg agttcaacca agcaattgta cagttaggta tgaatgacgg agcagttaag
    13921 gcagccactt ctacagacac attgagcggt tcttgggagc agatgaaatc cactgtaata
    13981 aatgggctac aaagtattat agaaaaaata ggcgttgaaa atatcactgg tttcatcaac
    14041 agagtaacaa aagggattga aaattctatt cctaaaatta ctcaatttat aggttggttg
    14101 agagatattg gaacgtggat cgttgaaaat agagagccac taacatggat tgtcggaatc
    14161 ataggcggaa ttacattagc agtaaaagca ttgaacgtag caagtatgtt gctggcaatt
    14221 actggcggaa cattggcagc cccttttgtg gcgattggtg tagcattagg cgcactagca
    14281 ggtgctttgg tgatagctta tacaaaatct gaaacattta gaaatatagt caatgcggct
    14341 tttacagctg tgaaaaacgt agttatgagc gttatcaata atttggtgga atactacaaa
    14401 atgttgtgga gcgtgttgca gtggctttgg gaaaaaataa aagaatgggc ttcatggatt
    14461 ggcaataaat tcattgaaat gaagaacagc gttgtgaata cggttcagaa cctatggaat
    14521 ggcgtgaaaa acttcttcag caatggcgtt ggagatacgt ggaataaggt agtcggttgg
    14581 gtaaaaaaca ttttcaacaa agcaactgaa ttgaagaaca acgtttctga tgtaatcggt
    14641 aacctgtgga atggtatcaa agacacattc cgtagaggta tcgatacggt attcaattgg
    14701 ttttcagaac taccaacgaa gatgaagaat gccattattg gcggtaaaaa cgccattgtt
    14761 gatgcgttca aaagtatttt caacgcagca cttaaagcga taggtaaacc agttaacgca
    14821 atcatccatg gagcttcatg ggtactagaa aaattgggcg cagaacctct acaagaatgg
    14881 gatgtaccac aatacgctac aggtacacca gcaggtgggc acccaatcaa tggtccaatg
    14941 atggttaatg atggacgtgg agcagaaaca gttatcacac cagatggtag agccttcatt
    15001 cctaaaggac gcaatgttgt tctaaacgca ccaaaaggaa cgcatgtctt gacagcagaa
    15061 gagaccgcgc aacttcaagg ttctaaagct cctaagtatc gttacaaaaa aggtactaac
    15121 ttctttggta acatgtggga tagtgtgaaa aaggttgctg gtaatgtagg tcacacaatt
    15181 aaaaacgtag taggtgacgt gtgggacttt atttcagacc cgggagcgtt agctagaaaa
    15241 gtacttgggg ggttagatgt attaggtgga ttgacaaagt atccattaga agtaggtaaa
    15301 ggcatcctat ctaaagcaac aagtgcactg actgaaaaaa tcactgggtt gttctcatct
    15361 ggtaacttag atacctccgt aggaacaaat ggcgtctata aatatttggc tgatgttgct
    15421 aagtctgtga tgaagaaatt cccaggattt gtggcaacta gtgggtatag accaggtgac
    15481 ccttattcac atggtaaacg taatgccatt gatattgcat taccaggcgt cacaggaggc
    15541 tcacctagat acacagaagc agcaaactac gcttttgaca aattcgcttc caaaattggt
    15601 tacgtaatca ctaatgggaa agttcgtgac cgttcaggac aatcaggtca accagcaact
    15661 ggtgcatggg agccatggcc cgatggagat cactatgatc atgtgcattt aaacggtgtg
    15721 aaagacccac aaaacactca aatttcagga gatagcgtgg gaggcagtgg ggtagaaaga
    15781 tggcgcaatg tagcaattag agcgttgaaa atgaccggtc aatacagtac tgcaaactta
    15841 aatgcattac taaatcaaat gcgtacagag tcaaatggta atcctaatgc agttaacaat
    15901 tgggatatta acgccaaaaa tggaacacca tcaaaagggt tgctccaagt gattgaccca
    15961 acattcagac agtatgcaat gccaggattc aacagcaata tttttgaccc actatctaac
    16021 atcttagctt caatcagata cgcactatca agatatggct cactaacaaa tgcctatcgt
    16081 ggagttggtt acgcaaacgg tggaattgta aaccaacatc aaattgcgga aatcgcagaa
    16141 ggaaacaagc cagaaattat tattccgtta gataaggcta aacgatcaag agcgatgcaa
    16201 ttattgtcta tcgcaatgga caaactagga gtttctccta aaacttatgg aaatgcgaca
    16261 actgtttcca gtgattacga aatcctagaa gccattgaaa aacaggcggc aacaacgaat
    16321 cagctgttat cgttattgct ggcatttttc aaaggaaata gccgaactga tagagattta
    16381 gctttagata ttcagaagat cttggttagg aggatgtaga tgcgaactgt attactgaga
    16441 aatagaataa acgaagaaat tgatttgtcc acagaagact attttgctac tgggttgagt
    16501 aatatggggt ttgaagtaaa aaaagaacat gtgggacagt gggggaattt cagagaaagc
    16561 agtgagagcg ttgaaatatc tgaatttcag tcatctgtaa tcatttctgt gcatgggttt
    16621 cgagaaaaag aactgtacaa ttcacttgtg cagttcctat cggaaggtcc gtttgaactg
    16681 gaatttgctt ttgacggcga aacaatggta agaagatgca gtctaaaatc tttaagtaag
    16741 actgaaatcg atccgaaaac atcgttacta acagacactt tagaactata tttcacatca
    16801 aactggtatt ccgtaaaacg cgaaaaatta attcagcgac cgaatgtagt gaaaacacgt
    16861 ggtaaggttt ttccgtataa aagatcttat atctacactc aaaacttgtg ggagaaaaaa
    16921 ggtgtattca aattcaataa taattctgta tacctaacga atagcaagga acggatgtcc
    16981 ccgctaaaaa ttcgtgtgat tgggaagtgt tcaaatccgt attgggaagt aatccaaaac
    17041 tcacaaatca tcgcaacgga tggatacttc atagatatga ctgaaacaca gactctagaa
    17101 gtatcaagcc tttttgaaga tacaacggcg attttaaaag atattgcagg ggtagaatct
    17161 tctgtctacc aacaacagga ttatacaaaa actaattttg ttcaggcccc aactggagaa
    17221 ttcagcattg tgtttcatgt tgggggggca gacgtagaga ttgagttata cgaggagcgt
    17281 gatctgtttt gattttagca gtcacgcttt ttcatcgtga tttaatgctt tataacgaac
    17341 atttattctc taacagtttt gaatttggtg tggacgagat caacgaagag gctagtagct
    17401 ttacgatgga taagtacgtt cctgtaaaaa caggcgattt tctactagca aaatatattc
    17461 ctagtggaaa atttgcatat tttggtgtaa ttacgtcgca ggaagacgaa aaaatcagtt
    17521 gtaaaagctt acttagttta gctgatagtg agataccgac tgcacgtgtg tcaggagata
    17581 actacgaaga gcatatccgg cggttgattg aatactattt gctgaatgat ccaacgaaac
    17641 aactaaaaga tattttagac gtcaaagcag aaagtgcgac ctctcattcg tatcaagcta
    17701 ccgatacgaa taaacacaag ttaagtgcgt atattctcaa tggtttcaaa aaatacaacg
    17761 taaaatggta tttcaaggga attcaaaaca gaaaaattta tacaggcata cgtgctgtaa
    17821 atgaatcaat ttacattaaa gacaattctt ctgaatttag cgattgggat gtgtttgttc
    17881 aggcgccggg cgctggaaac gagaacaagc tattaatcgt tgataaagca atgaaagata
    17941 tagagaaacc gataatactg tcaacatggt atttggacga agaaaacaat ttgacacaag
    18001 atggatcgaa agaaaatatc acgaaaccaa ctgtcaattt ggttaatatc tacgatcaaa
    18061 ccgcagaaga taaagcatcc tacgaagatg tggcaaaatc agagttgaaa ggcaatacgt
    18121 attcgcatga aatcaaagtg aacgttgtaa gaaatgcaaa aaatttgaat gtcgaaacga
    18181 ttgaaacagg gatgtttgcc acgatttctt ataaaggaaa gatatacaag tcggttttga
    18241 cagcttggcg aatatcaagt gataaggagt ttgtggaatt gactttcgga aacataagaa
    18301 gtcgttttat ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg
    18361 gatatcaatt tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt
    18421 tatataatcg gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa
    18481 gcggattaac agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg
    18541 ttcgacaaga agaaagtata acagttccag caaactcaag tggttatata gcattaacag
    18601 tggatttgac acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat
    18661 ggactaataa tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca
    18721 atggtgataa ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt
    18781 caatctcaaa gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga
    18841 gggggactgc gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga
    18901 cagtttcagg ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata
    18961 gatatgtaac tacgcctttt tataaggagc gtgtaacttt tactaatgtt ttaggagaaa
    19021 ttgtttcagg atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt
    19081 cgaatactaa tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg
    19141 tgcttaggta tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat
    19201 taaattatct gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg
    19261 tgatgggggg ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt
    19321 tgtaattaat ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac
    19381 cgagggagaa ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa
    19441 gtcaaatttc agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa
    19501 cggcaaaaaa gtaacttttc agaacttcat catagacgtg ttagaacgag tggatattaa
    19561 ttcggagcaa gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac
    19621 cactttcaat cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata
    19681 taacgaatta gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgatca
    19741 aaccaatcag caaatcggcg atctcggcaa gctgaaaaag atgtacagta acagcatcga
    19801 ctttgggggc tatgattatt cggggaatcc gaatttgtta agtaagctat catacgactt
    19861 aattgaaaat caaaatactt cagctggaac actttctaag ggtgaaaact cgtttaaata
    19921 taataagata tcagctgaag tggaaggtgg agtagagtta tattataaac gaagaggtat
    19981 agctaactgg ttaccctcta ataaaacgct tgtaatgact gttaagctta gagctggagc
    20041 ggactatagt ccagttgacg gaaaacttat actgattaga tataggtatg ttgacagtgg
    20101 aactggcaag attgttttag acttacctat taacagtaat tcgataactc aggaatggaa
    20161 agagtttagt attactggaa ctactccaac atttagccca caagcatacc atccttggat
    20221 acaatttagg gctcaagatg gggtacttgg ggaaatagaa atgagctatg acatcaaaat
    20281 cgaagaaggc tctacagcta caccatatca gcctaactta cttgatgact cttactggct
    20341 aggtaaaaca ccgttgggtg agaatttgat aacgaatgcc aagttcccaa ttataacacg
    20401 aagcaatcct atttctggtt ttgatatttc agaagaatta ataataggtg aaacttatac
    20461 ggtatcttta aaagggacta agccagctac taaagaattc cacttatact acggagaagc
    20521 aaattaccag cagttgcagt atcaggcaac tttacttcct gtggaaggat taacagatgt
    20581 gtggagtgcc acttttacag ccagaaatac tagtgaaacg acaaatttac ttagagtagc
    20641 gttatggcaa aaacctaatt ctgcaacata tgatactgtt caaattgact ggctcaagat
    20701 tgaaaaaggc gacacacgca caccaaatat cagtcagttt aaatactttg gtgaaggctt
    20761 gaaagacagt aacaatccga acgactacag ctgggatgtc acaccagagt atactgaaaa
    20821 aagcttgaat aatacggtta gtctgactga accgcaatcc atcgaaggat tgaaaaattt
    20881 cgaagatggt attcaatcaa aaggaaaatc tgtattgaca tcagacgaca acaaatatga
    20941 agtcgtaacc ttaacagtta caaacgggaa taccggatca gcaaagcttt atcgtgaagg
    21001 aaaaaccgtc actatttatt tttttgcgtt gaatgggaaa agcagtggtg gaaatgattc
    21061 aacgatacta acaattccag aaggctatcg gccaccaatt agttttgagc aactggttgg
    21121 ctcgatagac cgttctactt tgaacagtgc tcagttatct attggtgcag atggagccat
    21181 taaatggcga agaaactcaa gttatggatc ggattatacc tttgcaatta cttacacgat
    21241 ttagaaaggc gtgaatcgat atgaaggcag catatagacc aattgaacct tacggattcg
    21301 agcaaatcat tgtgaatgat gaagaacatt taccggaaga atgcacagaa gtcgaaccac
    21361 cgattccaaa ttggaaaccg aaattcaatt actgggaggg aaataaatga aaaacatttg
    21421 gaaatatggc cgtactggcg gagagtatgc aggaaaagta ttggacgaca tgcttgtatc
    21481 cgttccttac acagatcagc caccgctcga aggggttcgt tcagatggcg aaccgctaac
    21541 gattgctgat cagatgtttg atcctaaatt gaaccaatgg attattttag cgaacgcgtt
    21601 agatcacaac gatttaaaca atctcaaagc gatgtacgag gctcttgaac atgaaaacgg
    21661 caacctaaaa cagctaaatg ccaaactcat gctaagcgat gtagcgatta aacaggaaaa
    21721 tactgcattg aaagaaaaag ctgacagttt agcacaaatc aattcaaaaa tgatgcttgc
    21781 ttcgttacaa aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa
    21841 gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    21901 ttactgacga tcaagttcgt ctattcgttc cactatgtat cacagatgaa gaagcagata
    21961 gaattattag caaagaagag agcgcatctt aagtgatgcg tttttttgtt ggaaagttgg
    22021 tggaacatga aagaagaagc gctccaagac gttgtggaga gattagtaag aattgaaaca
    22081 aaattagaca actacgaatc acttagagaa aaagctgata gtgcaaaaga tttggcagac
    22141 aaagcctact cagtagcgct aaacaacgca gaagatatca aagaaatgaa agccaataat
    22201 aaatggtcgt ggggttacat gatcggttta ggcattacga tcattggcta tttcttgact
    22261 aaattgtaaa ggagttaaga agaaatgatt ttacccgata agtattatca agtcattaaa
    22321 tggacggttt taacagtatt accagctgca tctgttttag tagccacgtt aggaaaagcg
    22381 tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc aacattttta
    22441 ggcgttatca ctggtgtgtc tgcatataac ttaaaaaaat aggaggaaaa aaatgaagaa
    22501 gaaaatcatt ttatcattga gcctaataat ggctcttttt ttattgccaa ttaacgggtt
    22561 cgcctatacg atcaacaatg aatttaattt aggtgtaaat gaaggtagct cacaagtagc
    22621 aaataaccaa tacatcctat tgcatgaaac agcaaacgaa actgcgacag gacgaaatga
    22681 agcgcagtac atgaaacgtt cttggtacaa tgcctataca gcgtatattg ttggcgatgg
    22741 tgggatcgtt taccaagttg gacaacctgg ttatgtacaa tatggcgctg gttcatatgc
    22801 taatgcaaat agtcctgtgc aaatcgaact gcaacatacg cacgataaag taacgtttga
    22861 aaagaactac aaggcatatg ttgaattagc gagagattcc gctaataaat acggtattcc
    22921 acttacatta gacacaccgt ataatcaacc aggaatcaaa tcacatttat gggtaactca
    22981 aaacatttgg ggcgatcaca ctgatccgta tggatactta tcagaaatgg gtgtaagtaa
    23041 ggaaaaactg gcttacgatt tagctcatgg atttacagac gaaaacccaa cgacttctga
    23101 gaacaagcct gtcattgatc caacccgagc aggtgcagca aatccaactt tatcagatgg
    23161 aacgaaccat tctcacattg atcagtttgg agaaatcgaa aatgcgaact tacacgtcgc
    23221 tggatggcac atcgctaact ataaatacga gtatattttc attatggact acaatactgg
    23281 gaaagaatta gctcgagtaa gagctgatgg aatttataga ccggatgtaa atcaagctta
    23341 taatacttct ggaaacgttg gctatcatgt atcttttaac atgcgcaatt tccctaataa
    23401 gaaagtatac gtcatgatgc gggcaacgaa tgatc
//
LOCUS       DANKAY010000037        23412 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000037 DANKAY010000000
VERSION     DANKAY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(33..1076)
                     /locus_tag="PWY98_001437"
     CDS             complement(33..1076)
                     /locus_tag="PWY98_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2037196.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="PWY98_001438"
     CDS             complement(1237..1920)
                     /locus_tag="PWY98_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDL2037197.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="PWY98_001439"
     CDS             2115..3044
                     /locus_tag="PWY98_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2037198.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="PWY98_001440"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="PWY98_001440"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL2037199.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="PWY98_001441"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="PWY98_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2037200.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="PWY98_001442"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="PWY98_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2037201.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="PWY98_001443"
     CDS             5429..6232
                     /locus_tag="PWY98_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2037202.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="PWY98_001444"
     CDS             6316..7863
                     /locus_tag="PWY98_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL2037203.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="PWY98_001445"
     CDS             complement(8160..8357)
                     /locus_tag="PWY98_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037204.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="PWY98_001446"
     CDS             complement(8433..8633)
                     /locus_tag="PWY98_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2037205.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="PWY98_001447"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="PWY98_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="PWY98_001448"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="PWY98_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2037206.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="PWY98_001449"
     CDS             complement(11515..12297)
                     /locus_tag="PWY98_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037207.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="PWY98_001450"
     CDS             complement(12362..13198)
                     /locus_tag="PWY98_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2037208.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="PWY98_001451"
     CDS             complement(13216..14625)
                     /locus_tag="PWY98_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2037209.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14639..16462)
                     /locus_tag="PWY98_001452"
     CDS             complement(14639..16462)
                     /locus_tag="PWY98_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2037210.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(17305..17631)
                     /locus_tag="PWY98_001453"
     CDS             complement(17305..17631)
                     /locus_tag="PWY98_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037211.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(17677..18039)
                     /locus_tag="PWY98_001454"
     CDS             complement(17677..18039)
                     /locus_tag="PWY98_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDL2037212.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18076..18290)
                     /locus_tag="PWY98_001455"
                     /pseudo
     CDS             complement(18076..18290)
                     /locus_tag="PWY98_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18418..18753
                     /locus_tag="PWY98_001456"
     CDS             18418..18753
                     /locus_tag="PWY98_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037213.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(18829..20826)
                     /locus_tag="PWY98_001457"
     CDS             complement(18829..20826)
                     /locus_tag="PWY98_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037214.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(20924..21199)
                     /locus_tag="PWY98_001458"
     CDS             complement(20924..21199)
                     /locus_tag="PWY98_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2037215.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            21431..22165
                     /locus_tag="PWY98_001459"
     CDS             21431..22165
                     /locus_tag="PWY98_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037216.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            22204..23346
                     /locus_tag="PWY98_001460"
     CDS             22204..23346
                     /locus_tag="PWY98_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2037217.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         7223 a         4623 c         4094 g         7472 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
    17041 ctgatttatt tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa
    17101 taccaaacat atccataata attttttctt tttcatttct tttatattgt tataacagtg
    17161 aataacataa aaattcccga caatcattgt aatatttttt tgtcacctgt attagtattt
    17221 aatttatagg aaactataaa aactccctag tgaatttaca cctttgggag ttcctgtttg
    17281 gacggtatgc ataatacatt taaatcaatt taacattaac acctcagcat atggtatctc
    17341 cacatatata cggtctttct ttggatagta ctcaatattt atgttgatag cacttccatt
    17401 gtctaatata gctttagccc ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc
    17461 atcacatgat cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca atggtttcga
    17521 tgacttctct aatccagatg tacctgtcat ttttgaaaaa atgataccta taatagccaa
    17581 gacaacgata actataatga taaacttctt gttcatattt actttattca actttttatc
    17641 tcctatctcc aaaataattt gctcttatcc ctcttatcag tacattgaca tccctaaaag
    17701 tacaccaaag attgctccag ctacccctgc tatcatcaaa atcgtaccca tttgttcatc
    17761 tttatctcga tataagtagc ccataataac agcaacagca ccaaatacaa caggaataaa
    17821 tgctaacgct actaccatag aaatccaacc aataatcaaa tacagcttcc ctgtctcttg
    17881 tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt tctttactgc
    17941 gtctgctcca gttgtctcat ctgtttcttg tagtgctccg caaaactggc aatacttact
    18001 ttcatctgtt atttctttgc cacattttct acaatacatg tcttctcctc ctaaaaacat
    18061 tcaattgttt attagttaac tgtttacttt attaaataca tcagaaaaca gctcattcat
    18121 tggtacactt aatatctcag aaagcatctt catttgatag ggtttaggaa tcgttctccc
    18181 tgtttgccac gaacttacac attgctgtgt aacattcatt tttttgctaa ctccgtttga
    18241 gaaagtcctt tttcttttct gtatcggctg agattactta cttttttcat gattctcccc
    18301 tcctgtttta ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaaactt
    18361 caaataccat tacaaataca acaacttgtt gtactatact gagatggggg tgtattaatg
    18421 aaacagaaac aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa
    18481 aaagaattag ctgaaaaaat cttctctaca caacaggcag tagcaagatg ggaaaattct
    18541 gtaacagagc ctaacctcga tagcttaacc gctctttcta gagccctagg aacccctgta
    18601 agccattttc tagacaatgt tgtcgtagat tatgaggaag agtttttggc tctctatcgt
    18661 tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt aaaaaggcaa
    18721 gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc gaactttaac
    18781 ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct agtttaatac
    18841 aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac ttgtaaattg
    18901 ataaagagga actaattcaa ctcctgataa tatcaattct tttattactt catctatccc
    18961 tgctcctttt gctaggtaga ctaaaaaact actacttgct ttcacattgt atagtctctt
    19021 aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc ctctgttttc
    19081 ttttaaagca ttttccacgc tgttattccc aacattacgt agacgttcac aaacagtgtt
    19141 agtaagttct ttctctgtca ttagatatgt tccttgccac gataagccct gtaaaacttc
    19201 atgtgcttca tctaaaatca attgtttttc cttgctaagt tctgatcggg aattttgagg
    19261 aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat aagaaaaata
    19321 ttgatatgta aaatgaaatt tttccagaag tggaaggata tcttcattca atttttgaat
    19381 cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat gattaaagtt
    19441 tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca aaggaacatc
    19501 ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta acttattaca
    19561 tttatctatg tcataagttt taccatagta aaaactcatt tttttagcta attgttcccc
    19621 cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat cagaaagcaa
    19681 gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga aaagtttgtc
    19741 tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg
    19801 tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc gactgtcttt
    19861 ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat tactggaatg
    19921 acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg gattatgatc
    19981 gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca tatcaatggt
    20041 taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca ggcagtcttt
    20101 aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa aatcggtttc
    20161 attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac atgtcatggt
    20221 aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta ttatcactgc
    20281 atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt catcacaccc
    20341 ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt cagtaattgc
    20401 ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc gaatactttt
    20461 ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt ctatgcttaa
    20521 gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt taacagtgtt
    20581 atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat tgcctttatt
    20641 ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa actcatctct
    20701 tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt ctttttttgt
    20761 aactggcttc tctaataatt tttctttatc tacataaatt ggaatattaa tcattgatgt
    20821 ttccatttgt gtttcctcct tataaaattc tgttaaagac taaaacatac agataataaa
    20881 aacaaactct ttattactat atgttttagt cttatttgtt tactcaacct ttataccgtt
    20941 ccatgaaact atcaatatca gattttttaa atctaatgac cgtatcaatt tttataacag
    21001 gtaacccgta ttgagaaaca tattttctaa tcgttcctac tgatacacct agatattcac
    21061 ttgcctgctg atagtccata tactctagag tgactccaga aataatgtct tctagcatag
    21121 gctttagcac tgtaattatc tctttcactt gttgttcaac ttgtatagaa acaatgctat
    21181 caatgatttg tagagacata tttccccctc ctttcgtgat tgcttgaaca aattttctaa
    21241 gaattatctt cgtatttttt gctcaaaata aaagtatttt gttattaacc gattaatcaa
    21301 acaaaaaatg attaccagtt catcgacata cacataatac ctcgataaaa aagaggtgtc
    21361 aatatctttg taaaatttat ttacaacacc cctttttcga ggtatactaa ttttaggagg
    21421 tgtggtcaat atgataaaac gtccaccaat aaattattta gaacgaaaaa agatactcgg
    21481 aacaaaaata aaagctataa gaaagagtaa aaaattaaca caaccagcat ttggtcttat
    21541 gattaacaac ggtcaattaa ttgacaaaaa aactatctac gaatgggaga agggaaccta
    21601 tttaccaata ccagagcgat tatcccgaat tgctgatttg ggaaatatga gtatagagga
    21661 gttagtttgt ggcaatgtag aagaatatat tttgggaatt attctttata gagatagcat
    21721 tgtactagat ggtataacat ttccagataa aaacctgttc cagcatcttc gtcaacagtt
    21781 cccaccagta cactctaatt tagatacatg gctggataga tattccaaat tagaaccaga
    21841 gatgcaagaa tttattgcta acaagacttg taataaagtt aaaaatgaaa aaattagtct
    21901 tttcaatatc ttaaaaatag aagaattatt catcaatgct attgtagaag aatttgacaa
    21961 taacattctt ttcctcacta gcagtataga agaactacta gaaagaatgg tagatgaatg
    22021 gctacctatt caattgaaag acatgtctta tcccgaagaa gctgtgagag aaattactga
    22081 taatataaac aagctagaac aaacgatttc atcaattggg aaaaaataca ctaagaaaaa
    22141 aatgaaggga ggtgatacca tctagtggta agtactcctt gtaaactaag aggagatgat
    22201 ttcatggcaa aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa
    22261 ggacacattg ccaccgatcc acgaactggt gaaaaaataa atactacgag aagaggtttt
    22321 agtagcaaac aagaagctca aaaagattat gacgagtaca gacatcaaat attatacggt
    22381 gttaaaaaga aagcacctga tatgacattt gaagacctgt ataatgaatg gataactcat
    22441 cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag taagatacgc aaataatcaa
    22501 atacttccag catttggtaa gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa
    22561 gtggtagatg aatggcacag taaatatgaa agttacgatt atatgagaaa acagactgca
    22621 caaattttac gctacggggt tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca
    22681 cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa agaggagctt
    22741 aataatttac tggatgcttt taaagatttt ggtaacatga aacaatacgc cttcttcaga
    22801 ttattggcat acacaggtat gagaaagagt gaagtactcg ctttacagtg gaaagacata
    22861 gacacgttta ataaagaact acatgtcaat aaaactcttg ctgtagatga atttggtaaa
    22921 gttataattc aatctccgaa aacaagagct tctagacgag ttatctcttt agatactgag
    22981 actctttcta tcctcaacaa ctggaaatta cagcaaaaag aagagtattt aaaacttgga
    23041 tacaatacta gctctaaaga gcaacatgtt tttacaacag ttaaaaacac tctatacatt
    23101 ccaaacactg ttaatgattg gttaagatat attttgaaaa aatataactt gcctcgtatt
    23161 acacctcatg ggtttagaca tacacatgct agtttattat tagaagcagg agaatctgtc
    23221 aaggtagttc aacaacgatt agggcatgaa aatagtaaag tgacattaga catctatgca
    23281 catattacaa ataatgcacc taagaaaaca ggtcaagact ttgctgatat gatggctcat
    23341 cagtaaaaga gctggtcaaa atctggtcat ttctagtata caggcaaaac aaaaaagcta
    23401 gaacccttat at
//
LOCUS       DANKAY010000038        23325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000038 DANKAY010000000
VERSION     DANKAY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..2140)
                     /locus_tag="PWY98_001461"
     CDS             complement(<1..2140)
                     /locus_tag="PWY98_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2037218.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLK"
     gene            complement(2497..3870)
                     /locus_tag="PWY98_001462"
     CDS             complement(2497..3870)
                     /locus_tag="PWY98_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2037219.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3870..4265)
                     /gene="rbsD"
                     /locus_tag="PWY98_001463"
     CDS             complement(3870..4265)
                     /gene="rbsD"
                     /locus_tag="PWY98_001463"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2037220.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4293..5210)
                     /gene="rbsK"
                     /locus_tag="PWY98_001464"
     CDS             complement(4293..5210)
                     /gene="rbsK"
                     /locus_tag="PWY98_001464"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2037221.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5318..6331)
                     /locus_tag="PWY98_001465"
     CDS             complement(5318..6331)
                     /locus_tag="PWY98_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2037222.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6412..7011)
                     /locus_tag="PWY98_001466"
     CDS             complement(6412..7011)
                     /locus_tag="PWY98_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2037223.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7197..7898
                     /locus_tag="PWY98_001467"
     CDS             7197..7898
                     /locus_tag="PWY98_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037224.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7908..10619
                     /locus_tag="PWY98_001468"
     CDS             7908..10619
                     /locus_tag="PWY98_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037225.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11070..12698
                     /gene="treC"
                     /locus_tag="PWY98_001469"
     CDS             11070..12698
                     /gene="treC"
                     /locus_tag="PWY98_001469"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2037226.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12758..13939)
                     /locus_tag="PWY98_001470"
     CDS             complement(12758..13939)
                     /locus_tag="PWY98_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2037227.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14191..15714
                     /locus_tag="PWY98_001471"
     CDS             14191..15714
                     /locus_tag="PWY98_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2037228.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15783..16511)
                     /locus_tag="PWY98_001472"
     CDS             complement(15783..16511)
                     /locus_tag="PWY98_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037229.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16524..17804)
                     /locus_tag="PWY98_001473"
     CDS             complement(16524..17804)
                     /locus_tag="PWY98_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2037230.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17945..18547)
                     /locus_tag="PWY98_001474"
     CDS             complement(17945..18547)
                     /locus_tag="PWY98_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2037231.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18792..18998
                     /locus_tag="PWY98_001475"
     CDS             18792..18998
                     /locus_tag="PWY98_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037232.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19310..20029
                     /locus_tag="PWY98_001476"
     CDS             19310..20029
                     /locus_tag="PWY98_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2037233.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20110..20793)
                     /locus_tag="PWY98_001477"
     CDS             complement(20110..20793)
                     /locus_tag="PWY98_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2037234.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21011..23197
                     /locus_tag="PWY98_001478"
     CDS             21011..23197
                     /locus_tag="PWY98_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2037235.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7762 a         4202 c         4573 g         6788 t
ORIGIN      
        1 tctttaaagg cagatcaagc cagaatgatt tgtctaaact taacaaggtt agtacaggaa
       61 cagttgcagc tttaactgta cgagtagttg tttctggtat ctgtcttgcc gtaaaattgt
      121 tccacaaatc ccagattgaa gcgacacgga agatggaaga agcgttgttg acgcgtttta
      181 tcgttgaaaa ttctaatcct gtgattggca cgccatctgt tgtccatttg attggcacat
      241 cgaatcgctt agacatattg aggatttcag cgtttgcttc ttcagttgtt gctgcttctt
      301 ttttcggttc atcatatttt gtcaggtcgt acgcttcgat gatcgcgttc aatttttgtg
      361 aatattggcg atctgtagca taacgtcctt ccaaatattt ggttgcttct ttgtagtctt
      421 ttgtttccga cttccaagtt ggtttgtaga aatcaggatt gccagtcagt ccatttttga
      481 tcaagtcggc atagtcttcc aaactttcct tgtaagaagg atatttacgg aattgtgcgc
      541 ggatttggta gctttgtccg gaactatctt gttcaagtgt attgaaggtc acgctgcttc
      601 ctttatatga tcctttgata ccaaaaaggt tatgatttgg ttcgcttgat aataagctgt
      661 ttccagatcc actttcaagg atagcttgag ccatcatgac tgatgcgtag attccttctt
      721 tttgcccgat ttcctgtgca tcatctgcaa tctttttcaa gaactgatca gttgtttggt
      781 tttgactgaa atggaacgag ccatctaatc catctaaaat agatgagccg cctgtcagcg
      841 aacctgaagc agcaggtaaa cctgctaagt ttactcggac agcaaaatca ggcgtgaaat
      901 ctgaaataga agttactcga actttatctc ctggttgagg cgcatggata aattgtccgt
      961 tcccaatata gatacctaca tggtgagtaa caccggaaga aggtccccag aagacgaggt
     1021 cgccaggttg ggcctgactt actggaatct gcgtacctgc atattgctgt tcgcctgtcc
     1081 agccaccgat ataatgaccg gttgctttca tatatacgta ataagtcaaa ccagaacagt
     1141 caaaagtatt tggacctttt gctccccata catatggttt tccaagatgc ttgaatgctt
     1201 ctcgaacgat tgcttgttgg acattcgttg tatttgtagg caatacagca tcaattggtg
     1261 attttacaac aaaatcagaa gaagcagaag atggattctc tataccgctt tgatttgctg
     1321 tatttggacg agatgaagca ttgttcgttc cgccattcga tgaaccattg tttggatggt
     1381 ttggttctct ctgagtattt ccattgttat tcgatccaga tgggtttgtt tgattcgaag
     1441 aattcccgct gttcgaatta ttgttattat tgtttgaatt atttgaacca ttattattgt
     1501 tgttattacc cacatctggt ttgtttggcg tttccggatc tggtgtacta gggacttctt
     1561 cactaggatc agttggtttc tctgttgaag tatccggtcc agctttaaat tctgcagtaa
     1621 tagataaatt gttcgtacct actgtaaagg tctttgcatc ataatttact tctttggcac
     1681 cattaacagc gactgaagca atttgttcac cttcagctgg cgtaatagtg taagttacct
     1741 tcgtttccgc taataaacct tctaccgttc cactttccgt aatatccaaa ggttgctgtt
     1801 caggggaatc cgctgtcgaa gcagtcagtt ggatcttacc aatcaaagaa ggagcaattg
     1861 taagggaata tgtctcggct ggcttttcat tttctgatgg atcagccgga tcaatttcag
     1921 ttggctcggt cgatcctgtt gttgagttag ttgttccagt tgggtcttct gtaaaagaat
     1981 ccgttgtttc actagtagct gagctggatg tagtcgtatc tgttgtgact gtttgatcag
     2041 ctatttgatt atctacggca aatacggtat tggtcgacaa aacagacgga gcaatcagcc
     2101 aagtagcagc tagcaaacgt atcgtttttt tatttttcat aataaactag tccttttcct
     2161 ttcattgatt ttctgtctta ttcagcattc attagacatt ttttggaaca gataatcgtg
     2221 tcgaaaagct ttttcttttc ataaatctgt ctgtgtattc taaataaaac aatttgtaaa
     2281 taagcaacga gcattcattc gtttttcttt gacgttcatt ttattgcata gaccaccttt
     2341 gtttggggat aaacaagtgg ggataaaaat ggttatgcat atgtaaatta taccaaagtt
     2401 tggaaagtat cacaaaaatt tataaaaata atgatcctaa aaagaaaaag actgaaatat
     2461 gtgataaaca tctcatactt cagtcttttt cattaactat tcataagaca ttaggtcttc
     2521 atcaaacccg ctaacgagag tagtgatcat tgctacgact agactaacag ccagtcctgc
     2581 taagtaccag aaaaaagtac cggaaatgaa tgcaggcaat gtggtcagac taccaaatac
     2641 aaaagcgatc gctttcactt gggcaaatcc cattacagct ccaccagcag ctgcaccaat
     2701 gcaagcagca acaaatggtt ttttcaatgg gatcgcaatt ccgtaaacag ttggttcggt
     2761 cactcccgca agtaaaatcg aagcaaaagc agaaccggag atcgaacggg tttttgaacg
     2821 tttacttcgc agaaatacac ctaaagctgc accagccatt ccaaaattgg ctgctcctgt
     2881 caacggaacg attcggtcga acccataagc agagatatta ctgatcatga cagggtttac
     2941 agcccattgg ataccaaata ttaccaaaat attccaacca ccagcgatga ttgcgccact
     3001 gatcggtccg ttaatatcca tcatccaagt aattgcggca gctaaacctt cgccaaggta
     3061 aacacccgca ggcccgaata ccattaacgt caaaggaacc gtgataacta atgaaagtaa
     3121 aggtacaaat actaattgca tattttcgcg aatatatttt ttgaggaatt tttcaacaaa
     3181 agaataaaag aaaataccta aaatcgccgg catgacagta gacgtatact gcatcagtac
     3241 tacaggaatc cccagaaaat cagtagctgc tttgccactg tctgaaagta aagaggtcag
     3301 attcggttca atcagtgctg cagcaatgac agccgcaata tatccattca ctttaaacgt
     3361 tttggcagca gaaaaagcaa gaaagattgg cagaaaataa aagacggcat tcgacgcagc
     3421 agacaaaata tgataagttc cgcttgtttc actaagccag ccaatcgtag tcgctaaaac
     3481 aaccagacct tttaatatac cagaaccggc aaacagcgga atgatcggcg taaagatttc
     3541 cgatatcaaa ccaaatagtt tttcgatcgg atttagcttt tctttcggag ctgaatgatc
     3601 gatttgacgc gtcaattcct tgtaagcagg agccaatccc tgatccatga ttatttctgc
     3661 atatctatcg agtaaaatga aatacttaat ggaagggatt tgctgtttta atttttctgc
     3721 attggctact ggctgttttt tcaatttgat acgaagccga gtcgcacatg gaaggacaga
     3781 atcaatattg tcttttcctc ccaactgttc aatcagcata tgaatctgct cggacagtac
     3841 catctgttca ggattcattg tcgtttccat tagaagacca ctccactcac taatataata
     3901 ttggcgtaag gtgtcatttc gcctgtacga ataaatgcat gacagttatt caactctttt
     3961 ttcatttctt catgtgggat aaagctaatc ggtgtatctg gtaacaataa tttgattttt
     4021 tccaatattt ccgggttcat tgtctttatt tcttccgcca attcaatcga ttgtacctca
     4081 agttcttcta aaacgttttc taaaacagat aaaaaacttg gaagaccgtt tgagacagcc
     4141 aaatctattt tttcagtacc aaaggggaca ggcatgcctg catccccgat acttaattta
     4201 tcaaagtgcc ccatttgtgc gattactcgt gaaatatctg aattgattac ttttcctttt
     4261 ttcatgtttg tttcttcctc tcattgttaa ttttagactg ctgattgttc taaagcttcg
     4321 cggtaaggga tagaaggttg cgcaccataa gactgcacag caacagaaga ggctagattt
     4381 ccataaagga tcgcttcttt caagttacta aggtctggct gaagtgtgct gcttaatgcg
     4441 ccaataaatg tatcacctgc ggctgttgtg tcaacggcat ccactttaaa agcagggacg
     4501 ataccgtgtt ctttttctgt atgatagaaa gcgccggcac tgcctagtgt aatgattact
     4561 gttccaatac ctaattcatg gagtttttcg gctgctgcga ccaaactgtt atgatctgtc
     4621 actctgattc ctgtaatgat ttctgtttct gtttcatttg gtataatgat atctgtcttt
     4681 tcaagtagtt cagtaggaat cgtttcacga gctggtgcag gattcaggat cgttgttttt
     4741 cctgcgtctt ttgcaataga aaaagctagg atcgtcgcat ctaagctact ttcaaactga
     4801 gcaataataa agtcactgtt tgtaatcagt tgcttgtttt ttaggacttc ttgttcatgg
     4861 aaagcattat ttgcccctgc atggatcaag atactatttt ctcctgctgc gtctaccata
     4921 atcattgctt gtccggttgt ggcgccttgg atttcagcta tcccagatgt atcgatgttt
     4981 tcttttgtca gtagatctaa taactgttgt ccttcgctgt cagccccaac gcctccaata
     5041 aaagatgtcc gtgcgcctga acgtttggct gccactgctt ggttggcacc tttacctccg
     5101 ccagcgtgaa aaatctcatg cgcatgcatc gtttcgcctg gcttgggcat gttggttaaa
     5161 cgaagggtag tgtccatatt aatacttcca attactgtaa ccgtgttcat tttacaaacc
     5221 tccaactatc tcatttattt tgatgggaaa attatttttt aaaacgtttt agtaaaacgt
     5281 tttaatagtt cgataaaaat atagcacacc ttgaaagcta tttcaagggt gcagttgaaa
     5341 atcttttttc taatcgtaca ggtaatcttt tttcttccca ttctttttct gggaattgga
     5401 tccgatctaa caataacttg gcagatgttt gtcccaattc gaagattggt tgtgcgatcg
     5461 ttgtcagttt cggcttgata tactcgcaca tatctatatt atcatagcca ataatgctgt
     5521 agtcttcagg gatgcttttt ccagcttcct ctaatccgcg atataatcca aaagctaatt
     5581 catcatttaa agcaaacaca ccagttgcag cagaatcaag aagttctgct gtgatctggt
     5641 agccaccttg ttttgagaac tgtgtaggta agaggatcag ttggtcgtga gggatttggt
     5701 aaacatcaag agcagattta aacccctcaa tccgagcgtg gacattttcc ggtggatttt
     5761 caggatagac aagggcgatc gtttgatgtc ctagactaag caagtgcatg cccgctaggt
     5821 aaccaccgcg aaaatcgtct gttcgtacgg catcactaaa tccttcagat tttttctgat
     5881 ccaaaacaat gaagggccgg ccttgttttt tcaaattttc gttgattgca tctgtagaga
     5941 cagcagaagt cgcgataatg aatccatcca cacctcgacg tgtgagctcg gctagatatt
     6001 cgatttcttt ttgatgatca gaatcagcat tacataatat cgtgacgaaa ttctgcttat
     6061 acaagatatc ctcaattcct cgcatcagtg tactgaagaa tggattcgtg atatcaggaa
     6121 cgagtacacc aattgttttc gtttccttgg taatcatttg tctagcaaaa tagtcaggct
     6181 gatagcctaa ttcttctttg gcagctaaaa ctttctgaac agttttcgga ctgaatttct
     6241 ccgtatttcc atttaatatc aaggagacag ttgtgattga aacgccggaa agtttagcga
     6301 cttctttgat tgtgatcttt tttttcacca tgttcatact cctatctaaa tcctgtggtt
     6361 attttcccat gaaatcaaaa agaagtaaat gaaaatcacg tgtgccagta cttatttgct
     6421 ttgttggata gcttcgatta tttgtttcgt tgagcgtact cgtgcagttt tcggaaatat
     6481 cttttttatg aacaattgat gggattcttt gtcacgatca ctgcaagcat cttcagcaag
     6541 cacgatatgg tacccgtgtt gaaaggcatc taatgcagta gcatatacac cgtttgttgt
     6601 cgctacaccg cttaaaatga tagtttcgat tccacgccgg cgaagttgaa gatcaagaga
     6661 agtaccaaaa aaagcacttg gattgtattt tgtgatagtc aacatattat ctgtatcttt
     6721 caaagaatcc ttcaaaagta aatcagtgaa ttctggaggt acagaagcag tcattccacc
     6781 catatcagtt tgttgactca aataagggaa agtggagatg tctacgttca ctaaaacttt
     6841 caaggcagcg gtacgtttca atgttcttac taacaaatca ttgtttgtca gaatagatgc
     6901 tgcagtgtgt ggagagagtg taccgttatt tacgatctct ttttgcatgt cgatcgcgac
     6961 aaaagcagtt tttgataaat caaaaatcat attttccttt aaatcattca tgatgttcct
     7021 tctttcaaat aacaatgttt tttatgtaag taaatcataa atcgtttatt tgtcaactaa
     7081 attaaaggat tcctttcttt agtctagaga aattcagtgg taaaataaat aagtgtgaga
     7141 aaacgcattc gatgcataga tatagcgaga aatatcgtga aaatggagga atcagagtga
     7201 agtatgtcga agtaaaaaat gaatacaaac gatacaaaat gggagaaacg accattacag
     7261 caaatgacga tatttctttt tcaattgaaa aaggcgaatt agtgattata cttggtccaa
     7321 gtggagcagg aaagtcaacg attcttaata ttttaggtgg gatggataca cctgatgaag
     7381 gtcagatcat tattgatgac acagatattg cgcaattttc agataaacag cttactgctt
     7441 accgacgaac agatgtaggg ttcgttttcc aattctacaa tctcgttcct aatctaacag
     7501 caaaagaaaa tgtggaattg gcaacggaag tttctcctga tgcgcttgat cctgtagagg
     7561 tattgaggca agtgggccta gctcatcgat tgaataactt tccgtcccaa ttatcaggag
     7621 gcgaacagca gagggtttca attgctcgtg cactagctaa aaatcccaaa cttcttttat
     7681 gtgatgaacc gacaggcgcg ctggattttg aaacgggcaa gcaagtattg aaattattgc
     7741 aaaacgctag cagacaacac ggcaataccg ttttgatcat tacacataat tctgctttag
     7801 cgcccatcgc tgatagagtc attcacatta atgatgctaa agtaaggtct gttgaattaa
     7861 atgatcatcc ttcatccatt gatgagattg tatggtaggg ggattttatg aaaacaagaa
     7921 catatcttaa agcaggtttt cgcgaaattc ggcaatctaa aggacgattc attgcaatca
     7981 tccttattgt catgctagga acacttttat ttgtaggtgt caaaactgct ggacctgtca
     8041 tgcaaaaaac gatggatcac tatgttggta cagcaggact ttctgatttg caaatcgttt
     8101 ctactggtgg cttgacacaa aaagatattg ctcaagcaga aaagatccca gatgcaaaag
     8161 tagaaacagg aaaacagatc tattattcca atactggcaa aaacgaagtg attcaaatat
     8221 tttcttataa caaaaataac aagcagaaca agctacaatt aactgatgga aaactcccgg
     8281 aaaaaccaaa ccagcttgta ttggatgaaa aagcggaaga tgaagggtat aaaatcggtg
     8341 atatttatca gattgattca gatgaattga aagaaaaaga gtataccatc acgggctttg
     8401 tccgttctcc attgttcatc aacaatttgg agagaggata cgcgaacgtc ggaaatggta
     8461 gcgtcgatta ttttgtctat ttacctgaat ccgtttttaa aacagatatt tattctgtct
     8521 tatatcttga ttttacaaac gtaaagaatt tgaatacgta cagcaaagct tacaaagata
     8581 aaatggagca aaatcaagaa aaggcagaaa agtatttaca agatcgaccc gaagaacgct
     8641 tagaagagct gaaaaaatcg gccaacgaag aattagaacc ggcacagcaa aaaatagcgg
     8701 atggcaaagc acaaacaacg caagcaagaa cacggttaga agatgctaaa aaacagttag
     8761 cgcaacagtc agcaactatc cagcagcttc ctgaagcgca acgaacaatc gctgttcaaa
     8821 cattatctca acaagaagct caacttgctg aacaagaaag gcaattagtt gagaaagaac
     8881 gtgaactggc ctctgcagag gaagaattga atcgaaatgc agaaaaaatt gaaaatttaa
     8941 ctaaaccatc atacctatac aatgaacgtt cagaaaatgt cggttttcaa gaatttggtg
     9001 atctgtctga tcgtatcgct gcgattgcca atgtttttcc tgttttcttt ttctttattg
     9061 ccgcattgat cacctttacg acaatgacac gtatggtaga agaaaaccgc agggaaatcg
     9121 ggacattaaa ggcgttaggt tatacgaaag ttgaaatcgc aaaaaaatat gcaatctatg
     9181 caagtctagc ctcagggata ggtattgttt taggaacgat attaggaacg aatcttttgc
     9241 caaggatcat ttttgagctg tctaacgagc ggtatgatat tgggagtgcg cttatttttt
     9301 atgactggcc gccgatttta caagcagctg ccgcattctt cattgccgca tttggcgctg
     9361 caatgatcgt tttattcaaa gatttaagag aacggcctgc tgcattgctt caaccgaaag
     9421 caccgaagcc agggaaacgc atttttcttg aatacatcac tccgctgtgg tcacgcctta
     9481 gctttaatca aaaaatcagt taccgaaatc tcttccgata taagtcaaga atgcttatgg
     9541 cgattatcgg aattgctgga tgtgccgggc tgatggttgc aggcgtggga ttgaaagatt
     9601 ctctaagttc tgtttcagcc aaacaatttg gaccgatcat tgactatcaa gcaatcgtta
     9661 cattggattc atccacggaa gatagtcgtc aaaaagcagc ggatatgctg cagaagcaag
     9721 aaaaagtaaa tgagatccta gctattcata ctcaaaccgt agaattgaga aaaaaaggac
     9781 atgcttcgca aagtctgaca gtgatcgtac caagtaaaac agctgatttg gatcgtttta
     9841 tccatttaac tgatcttgaa gagaacccac gctccttgcc taaaacagga atcgctatta
     9901 cgcaaaaagt agcagaacta tttgacttat ctacaggaga tcatatttcg ctttatgatg
     9961 aagatcaaaa agaatggaaa gtcgaagtaa aagaaatcgt gaaaaactat ctaggtagct
    10021 ttatttatat gtcccctgaa tattatgctt cagttactga tcaatcgatg atggataatg
    10081 catttttgct gcgtacagat aagatgacaa caaaagaaga agaaatgctt tccgagaaac
    10141 tgcttgccac agacgctgtg acaaatacgt ctttcatgtc caaacaaatc gaaacgcaag
    10201 aagaatcaat ggcgaactta gactcggttg tcttaatctt tgtcgtatta tcaggactgc
    10261 tggcttttat cgtcttgtat aatctaacta atatcaatat ttccgagcgt gtacgcgaat
    10321 tatctacaat aaaagtcctt ggattttttg ataaagaagt cacgatgtac attgttcgag
    10381 aaaatatcat ctttactctt ttaggaatca tcggaggatt tggtgtagga tatatattga
    10441 ctgacttcat cttgcaacaa gcgtctatgg agaatgtcat ctttccatta gtgatcacat
    10501 ggtgggctta tgtcttatca gcaggattaa ctatcgtatt tacggttatt gtgatgattg
    10561 ttacccattt caaattaaag catatcgata tgattgatgc gttgaaatcg aatgaataaa
    10621 aacagaggtt gtgaaatcag cggtttgacc tgagtattag agaaacaaac ggcaaaatag
    10681 tttttcatat tttgccgttt tttgagctaa taccgaagga cagctgattg aacaccatga
    10741 atcagaggtt gtaaaaaagc cgtcctgcat cgagcaaaaa gaatactcaa ataaaattag
    10801 ctcctcatgt tttacatttg agaagactga ttgccgtaga taaaagcttt tgaacaccat
    10861 ttatagaaga aacaagaata ttttgctatt ttaaacaaga ggatagacaa atacatgtct
    10921 gatctcttgt tttttattgg gcaacagacc tcttagaatg tataaagata aattttacaa
    10981 acgctttcgg ttcagttata ataataaatg ataacttgta tatataagtt gtggtaggta
    11041 ataaatcagt tgaatggagg taaatatcca tgacatttaa agacaaagtc atttaccaaa
    11101 tctatcccaa atcttacaaa gacagtaatg gtgatggaat tggagattta aaaggaatca
    11161 aagaaaaact cccgtatctg aatgatctag gcatcgacat gatttggcta aaccctattt
    11221 atccaagccc tcaaaaagac aacggttatg atatctctga ttacacagcc atcgatccta
    11281 tctttggtac gatggaggaa ttcgaacagc taatagccga agcaaagaaa taccggatag
    11341 aaatcatgct cgatatggtg ctgaatcacg tttcgacaga acatgaatgg ttcaaaaaag
    11401 cactagcagg ggaccagcat tatcaggact tcttcatttt aagagaagag ccaacggatt
    11461 gggtatcaaa atttggtgga agtgcttggt cgccatttgg agatacgggg aaatattatt
    11521 tgcacttata tgatcggaca caagctgact tgaactggcg gaaccaagca gtaagagagg
    11581 aactattcaa agttgttcgt ttttggatgg aaaaaggggt aaaaggattc cgtttcgatg
    11641 ttatcaatgt tatcggtaaa gacgaacaat tgaaaaataa taaagaaaat gatggcaaac
    11701 ctgaatatac ggacaaacca atcacccata cgtatctaaa agaattgaac caagcaacgt
    11761 ttgggacgga ttccactatc atcacagtcg gtgaaatgag ctctacgacg atcgaaaatt
    11821 gtatcttgta tacagaacct agtagagaag aattatcaat ggtctttaat ttccatcatt
    11881 tgaaagtcga ttatgaaaat gggaataaat ggtctactcc tccatttgat tttgcagaat
    11941 taaagcgact atttcataca tggggagaaa agatgagtga gggtaacggc tggaatgctc
    12001 tattttttaa taatcatgat cagccacgtg cgctcaatcg atttgtagct atcgatcagt
    12061 atcgaaaaca aggagcagag atgctagcaa ccatgatcca tttgaaccgt gggacgccct
    12121 atatttatat gggagaagaa atcggcatga tcgacccaga ctattcatcg atttccgact
    12181 atattgacgt agaaagtttg aatgcttata agttactcct tgaacaagga ttcacagaaa
    12241 aagaagcgtt tcgtcggatc aaagcaaaat ctcgcgataa ctctcgaaca cccatgcaat
    12301 ggtcagacac agaacaagca ggatttacta cagggaaacc ttggttaaaa gttggaggca
    12361 agctagaaga gattaatgta gaaaaagaac tatcttctga agattctatt ttgtcctatt
    12421 acaagaaatt gattcggctg agaaaaacat atccaatcgt tgcacaagga gattatcatg
    12481 cttatgaagc aaatcatccg gaagtttatg gctttcttcg ccaatttgaa ggacagcagc
    12541 tgcttgtatt aacaaatttc tatgataaag atacaaggat cgccatccca gaaggattca
    12601 cagaagccaa tattcttatc gataattatt ccgatactgt tcttcatcaa agtctggagc
    12661 taaaaccata ccaggcaatt gctttgctga aaaattaaaa caaaaacaca aaacgtcgtt
    12721 cctacgagct attctcgtaa gatcgacgtt ttgtgtttta tttaaaataa tcttcttcag
    12781 aaaatgaaga gttttcatac tctcctttat cgccataaaa tttattgtag cgttgtttga
    12841 atttacggaa aataaaagta acgagtaatt tgactaacgt atagatagga ataccaaaaa
    12901 taacgcccac aattcccatt agatcaccca tgacaagtaa aacgattaag atcgtaatcg
    12961 gatggatctg taaacgatca cccattactc gaggaatcac caagtcaccg tggagaagtt
    13021 gtacggcaaa ccagacaatg acaaatttga cagccataaa agtagagtct tggaatgcaa
    13081 tgatcaaacc tggaaagaag gcgataaacg gcccaatata tggaatgata cataacaacc
    13141 cagcagaaat agctaaaaca ctagcaaatt ctaatccgat cagtaagaaa caaacccagt
    13201 agactgctcc aaggataaat gaagcaatga tttgcccctt tagaaatgaa cccaattgtt
    13261 gattagcaat ctttgtcaaa tttttaaaat ctgtacgaaa tgctggtggg acaatcgcta
    13321 aaactgactg ataaaatttt tgaggatctc tcaaaaggaa aaaagcgaca attggaccag
    13381 taaataaagt aataaaaata gctgtaacag ttgaaaaaat gttgctgatc cgttgtgcgc
    13441 cactttccca ataatttcca ataaatccag ctatatcaga cgtcacgtcg tcaatagaag
    13501 cgaaaaattg attaaatgaa tcggcaaaag gtgttttctc caaaaattgt cggagactct
    13561 cttgaaaatc ttgaaacaaa tctggcaaac tactgatcaa tgtctttgcc tgttcttcaa
    13621 gaaaaggaat aagccatatg cttccaagcc ccaataaaaa taaaataatc acataaacga
    13681 taatgactga caagttcctc gaaaggtgct tgctcaaacg tttgatgact ggttccaaaa
    13741 gataatacaa gatcagtgca aacaataatg ggggcaagat agtagcgata atgattccga
    13801 caggcttgaa tataaacgag atctgatgga aaaaataaat gatcagcgca agtaagataa
    13861 ctattcctaa aacatagtaa gaggtttttc ctcctaaaaa gcggatcaaa cgattcgttg
    13921 tatctgtttc ttttttcact ctttttcctc ctttatctat aaattcatta tagacaaaag
    13981 cgggtataaa aggaaaatga tagaggttga ttgcaaaaat atcgtcaatc attcatataa
    14041 actttctcat caattaaaat aagaaaacgc ttgacagttc ttttaaccat atgataaatt
    14101 aaatcctgtt gctaaaacgt aactattacg gtttgcgaaa agcgaatcgg aacattcaaa
    14161 tgaaagaaga tgagaagagg agtgtcataa atgaaacgat tgataggtat attggccatg
    14221 ctggttttag caggtatgtt aactgcttgc ggggcaagtg ggaaagccga ggacagtaaa
    14281 gaaaaattga gcgtgatgac gaccttttat cccatgtatg attttacaaa agcaatcgtc
    14341 ggggatgaag gagaagtaga gttattgatt ccagcaggga ctgattccca cgattatgaa
    14401 ccttctgcta aagacatggc aaagatccaa gatacagata tctttgttta taatgatgaa
    14461 aacatggaaa catgggtccc cgcaatccaa aaaacattac aagaaggcaa tgtccatacg
    14521 ataaaagcga cagaaggtat gcttttactt ccgggaagtg aagaaggaca tgatcatgac
    14581 catgaacacg gggaagaagg ccatacacat gagctagacc cacatgtatg gctagctcca
    14641 agtttagcaa tcaagcaggt agcaaacata cgtgatcagc tgattgaagc ttacccagaa
    14701 aaacaggaag tatggacaaa aaatgcagca gcctataccg agaaacttca agcactccat
    14761 cagctttatc aagaaacctt taagcaagca aaacaaagat cttttgtcac gcaacatgct
    14821 gcattcaact atctagcgtt agaatatgga ttgaatcaag tatcgattgc tggtctttct
    14881 tctagtgaag aaccgtctgc ggcgagaatt gctgaactga aacactttgt taaagaacat
    14941 ggaatcaact atatttattt tgaagaaaac gccaaagata gtattgccag aacgttagca
    15001 aacgaagcag gtgtatcttt agaagtattg aatcctttgg aaggattgac gaatgaacaa
    15061 atagaaaatg gagaaaacta tctttctatc atggaagcaa acctcgaagc gttgaaaaaa
    15121 acgacagaga cagaaaaccc gctagaagat actttgaccc ccaaaaaaga aaagagcgtc
    15181 tacaacggtt attttgaaga cgaggatgta caagatcgct ctctttcaga ttgggcagga
    15241 acttggcaaa cagtagatac gtatgtagaa gatgggactt ttgatcctgt ttttgaatat
    15301 aaagaaaaat tgaatcagga taagacagca caagaatata aagaatatta tacgaagggc
    15361 tatcaaacag atattgcttc aatcaagatc agtgagaaag agatggcatt tacgtttaag
    15421 aatggaacaa cgaaacgttc aaattatcgt tatgtaggga aagaaatctt gacctacgag
    15481 gcaggaaacc gtggcgtaag gtatctattc gaagcagaag ataaggaaag tggcgctttt
    15541 agatatattc aattcagtga tcacacgatt gcaccggctg actccgaaca ttttcattta
    15601 tatgcaggaa atgaaagtca agaagtattg ctgaaagaaa tggagaattg gccaacattt
    15661 tatccagata caatggatgg acgtgaaatt gcacaggaaa tgcttttgca ctgataggca
    15721 aaaaagaacc gaagggtttg gatcggctgg tcatttagtc ggtccaaact attcggttct
    15781 ttttatgaaa tcaatttctt taatccacgg atagaggctg ggatggtctc gccgtttgcc
    15841 aacgtgactt ctctggtttt tttattaatc gaccgcacat tttgtgtatt gataacatag
    15901 gatttatgac aacggataaa attccgatgc attttctcga tttctttgat cttctcatag
    15961 aattgtaatt gggcatgagt caaatggact tccagtttat gtggcgctcc tagcgaagta
    16021 gaaaaatatt ggatgtcttt gatcggtaaa aatttgatct catcattaaa ctctattttg
    16081 aagatatctt tttggatttg ggacatttga aaatcttcga aaattttgct caggatatgc
    16141 tcgacatttt cttttaaatc ataaagatct tctttgacga tataatcagt cggttcgatg
    16201 ttgcttttta agattttcat cgacatttcc gtatgggaag tcacatagat tatttttcct
    16261 aaaggatcta attcacggat caatttaccg atcgctacac catccatttc actgtcttct
    16321 agttcgatat ctaaaaagta aataccaaaa gaagaagttc tgatcttggc tgcattgaga
    16381 agttcttccg gatcagaggt agaaatatgg acataactgt ctaagttttc aatcatgatt
    16441 ctatttttga tcaccgttga taaaacttct aattgttttg gatcgtcttc acaaatataa
    16501 atgggaatca tatcattact cctttatttt atttgtatat aaatcgtttg agaaaaacgc
    16561 tggttttcaa ttgatgtttc aaggaaaaga taaggattgg tctttgtcaa ttcatccaag
    16621 ataaacaagc caaggccttc gttttttgga tttgtttttg ttgagaaccc tttttgtttc
    16681 aactggttta acggtaatgt atttgttgtt gtattgatca cacgaatcat cacggaatga
    16741 ttcttcttga gtatggtcaa ttcgattttt ttcttctttg cagcactagc tccttcaatc
    16801 gcattgtcca agataatacc aagcatacgt acaaggttta ccgtatgttt cgtatccagt
    16861 tcgattggct caggaaaatg aagctggacg atcaaccctt tttcctgtgc catcatgagt
    16921 tttaatgaca gtaaactacg gatctcagga actttcatat tatccaacat acttagattg
    16981 gccggaaaaa ggttcatcat ctggcgtgta ggtaatatcg tttgatcata ataagaccgt
    17041 aatccttcta agtcgccttc tgaaatatag ctgtgaagag aaaaaagcag attttggtaa
    17101 tcatgtttga attctctcaa gtcttgatag tttttttcta gatctttgat ataatcctgt
    17161 tgcatcctga cttgttgctc taataactga tcagctaatt tcttattaga gattttccaa
    17221 taataaacca agatgatcag gccaacacac agatatagaa tgaaaaaact cgtattcaaa
    17281 gctaagacat caggtgtttc accagaaaaa cgggtataaa aaataatgat atagtatgta
    17341 aataatatca atgtcccgat cgtaccgatc atacggcggt ccacagtgtg aaaccaagtc
    17401 tctaacgatt tttttaaaca aaaagctaat aaagcagata aaaaagcgga taagacaatg
    17461 tgaaaatact gcaatgaatc tcctggttca ataaccgaag atttcaaaac aaaaaaatcg
    17521 ccgatgcttg ttaagtgatc tcctaaaagc gacgtcaata acccaaaagg aagcgcgtaa
    17581 accaattgtt gacgcgaagt cgaaaagaag aaaaaagtaa gcatcagcat tcctatagca
    17641 attagagcag taaaaatccc taaaatcgaa tagacccatc caatcaaaat acaagaaatc
    17701 agaatgactc ccgcaaacag atatttgtat cggatgtgat ggtcaatcaa taagatacaa
    17761 attgtgtaaa tcaaatattg caacgctatg gaccataaaa acataaagta cagctcccat
    17821 tgatttttac caaattatag cacaattggc gtattatttt tttaattttt cagggatttt
    17881 gaattcagaa ctcataccaa agcagcagcc agatacaaaa aagtcagata agttagttaa
    17941 ttgtttaatg attgtttttt tcatgaaaga ttcctccaaa attcatcaag tagtttgtaa
    18001 taggaagcaa cgtagctatt tctaaaacaa tcccaagtag caaataggta gaaaagtaag
    18061 aagctgagaa aaataacgta atgagagaaa aaattagaaa catgctgatc gccttcttta
    18121 ataatggggg acgtttctcg ggttcaatgg gattgattgc tgtacctctt gatccatagc
    18181 gaagcagtaa caaaaagcag ctgattaaca aaaagaccat cattgggaag gagatagtca
    18241 attgcagatg tttcatcacc aagggaacgc cccataaata aagtagtgtc cacagcagac
    18301 aaacgaattc agagtcggaa tgcaatccat aagcatatct gcggatgcag atataagaaa
    18361 gaaaggtgat tcctgtttcc gcccatgagc ctgtcaccag agaaaagact agaacgaccg
    18421 ctagttttcc aagattattc aataaaactt ctaagacaaa ttttacttgg acatacacca
    18481 tatcttcttc cgatttgtct ccgctgaata aggcttctaa taatttgttc acaactttat
    18541 ctttcatctc atacgtacct ccaccacaaa ttttatgcta aaaaaaataa aaaacgtctt
    18601 ttgtttcata aaaagtggtt ttgacatcaa aaccgtcatg atctcctaaa aaaatcttcg
    18661 ttttccaaga accttaccaa aaaaagtgac agttttgata cgaaaagtac tttttatgac
    18721 aagtttgtgc tttttcgagt gatttatgtt aaaaaattac tgaccttata aaaggtgatg
    18781 gagggattct tatggaaaat gaacaattgt ttgaagtcgg cgaaacatat gcatttaaat
    18841 ctgatttatt tgaatcacca ataaaaggaa aaatagaaaa aatctatgaa aatagtgtga
    18901 tgatacgtgt catgaaatgc gcagtagaag acaatgatac cgctcataat ctgcactggc
    18961 agttagttgt acgcaaatca agtacaatag aagaataaaa acagataaaa tgttgagagc
    19021 ataaagaggt tgtgagtctc ccgtttagct ctgagttaca aggaataatg ttaaaaaaca
    19081 gcttttcatg ttttatgaat attgccttgt gaccgaggtg ttggcaggag aatactgttt
    19141 atttgaaaaa agggctgcga caagactttt gtcacagcct ttattttcat gaataggagt
    19201 tacttttggc tacttttaga gaagtagctt ttaaggagca gataaagtca aggcttttat
    19261 ttctataaat gaagtagtaa aatgaagaac agattttatt acaggaagga tgccgataat
    19321 tgtgatcatt gttgcaggaa cgatcggtgc agggaaaagc acactaactg aaatgcttgc
    19381 ccaagattta gaaacaaaac ctttttatga gaacgtggaa gataatgaag tattgccgct
    19441 attttattct aatccagaaa agtatacttt tttattacag attttctttt tgaacaaacg
    19501 ctttttggca atcaaagatg ctttcagcca tgatgacaac gtactagacc gctccatcta
    19561 cgaagatagt atgctgtttc atttgaatgc agatttaggg cgtgtttctg aagtagaagt
    19621 aaagcagtat gaaggtcttt tagaaacgat gctgaaggag ttagaagaaa tttcgccaca
    19681 aaagaaacca gatctactgg tttatattcg tgtatccttt gaaactatgt tagcgagaat
    19741 caaaaaaaga ggccgggaat atgaacagct agaacaagat cctgaactct attcctacta
    19801 taaagaactc aatcgacgat acgaagagtg gtatgaacag tttgatatat gtcccaaaat
    19861 agtgatcgat ggggacaaat atgatttcgt agccgatccg gcatgtggag aacaaatcgt
    19921 acaagaaatc aagatgcggg caaagaaaat gacagaagag gcagatgccc agtcgatccg
    19981 ctacactaca aataacccga aagcaaaggg aatctttaca actaaataat ataaaaacta
    20041 agcaaattgc tcactcgcag atatttctct gtgagcagca tcttgcttag tttttttgat
    20101 tcattgagat caaatagtga accgattcgt atgataaaat tgccataaat gaaatgtctg
    20161 agtgagggcc atgatcaaaa ataaaatgga aacgatcaaa atcgaatggg cgccaaacag
    20221 atgatgacag aaaacaagga caatagctcc tgcgataaag gcaagaacga cacttagata
    20281 gtggaaaaat cgatcaaaat ctgctttttc tttagaaata caataatcat ataagttcgt
    20341 tgctacccct tttaaatttc cagttgtaaa taaattagtg taggcacgcc cgttgatttt
    20401 atcaaaagct acccactgga tcgatgcagt aaaagcaatc aaaatcgtta ctaatgcggg
    20461 atgctcatgg acgggtgtca aactaacaaa gaaaaaaatc actgcttcat aatatagaac
    20521 aaacaagcgc cagaaatgtt tttctttttt tctaaaataa tcaattagat actttgttag
    20581 aagaatccct aagaaaaaga aaagggtaga caatactttt ttaccaacac ttagccattc
    20641 tccgtcaaat gcttggatga tcgatagaat gatattgcca gtttgagcag aggcaaaagc
    20701 accatattgg atataagtat aactgtccat ggctcctccg ataaatgtca gcaaaagtcc
    20761 tattgtacgc ccctcgtgaa aggaagtctg catttttttc atcacctttt atgaatattt
    20821 ttgtcttcct tatcttagca taaacgattg aaaaaaagga aaaattttca gaaaactaaa
    20881 atcatatcgc ttcccatttt catttttaga aaggtctgtt agtataaaga aaaaatagac
    20941 aacgaaactt taaataacca acttgaaata aacaaattca aagaagaaag atgggaagga
    21001 aatcaatcag ttgaatgaaa aagaaattct ccgattacaa aaagaattga aacgctggga
    21061 gaacaagtat aaaaagcttc aacaagtctt tcggctacaa gaagaaatca ttgctaccta
    21121 tgaagcggct tatggcgaag cacccggact tattgaggaa gaagtacaag taaaagaaag
    21181 tgaaggaaac agctctttct ttaaaatgat ggagaaacgt tcctataatc gcttcaatca
    21241 agaacaaaaa gcagctatca catatgatat gaagaaacat cttcgaatca tagcaggagc
    21301 tgggagcggc aaaacgcaaa caatctgtgc aaaagctgtt tatctgatga cgcaaaaaca
    21361 agtagacgaa gaacgcatac taatgatcac ttttacaagg aacgctgcaa atgaactaaa
    21421 aaaacgagta gataatttca gccagagaaa aaccgctgtc catatcggaa cattccatag
    21481 tatctttttt aggatataca atgaaatttg tcgaaaattt cctgaagtag caatgcaagg
    21541 aatacaagga gacttttcaa aggatactgc acaaaaagtg aatgctgttt tgcaacagct
    21601 gatacgtaaa tataatcttt atatatttga tcaatatgga gaaaaaacga ttgcttctcg
    21661 gttagactat tggcagaata tgaacctttc agtagaagaa atggttcagt tagttaaaga
    21721 aaaatatgat tccattgata agaatgcacg acagccgata tcagaacgac tgtatggatt
    21781 gctcacggaa ctccaagaac aaaaaagaag ccagcaattg ctagagttca atgatgtact
    21841 ccaaaatctg aaaagcgctt tggagaatga ggagatccag cattatatcg gccaaaaata
    21901 tgattatcta ttcattgatg aatttcaaga tactaatcca cttcaatggg aaatcgtgaa
    21961 acggatgaca aaggaaaaca ggatcaagtt gattgttgtt ggagatgatg atcagagtat
    22021 ctatggattc agaggatcag agccagcata catcaagaac tttgaaaaag aatatcctac
    22081 caaaactctt ttcttattaa ctaattatcg ttctcgggca gctattgtcc aaggagcaaa
    22141 tagattgatc agctataata aaaatgatcg gataccaaag gcgatgattc cagcacaaaa
    22201 agaagcaggt gttatggaag cctatttgtt ttctgatact aaagcagaaa gtcagtggct
    22261 aatcaaacaa attcagacat ttattcaaaa aaatgggaaa tataaggaat caatcatatt
    22321 ataccgatca gcttcacaaa cgcagcagct tgtccaatct ctgctgaaaa cgaatattcc
    22381 ttttgtactg gaagcagata gtccatatga aggtatcttt ggtatcaagt ctttccaaca
    22441 tttctatcaa aaaattgttc attggcaaac agcactgaat ctgcaaaaga aaaatcaggc
    22501 ctaccagcaa ttgctccggc agctgatggc agactgctac ctgaaaaaaa gtgaatgcga
    22561 tcaatacttt tctcctaaat atcaaaatat agccgttgca gattatattt tgtcgataag
    22621 ccccaattta aaatcgaaat ctgcagaaat ccgagagttt gaggaagcgt tgcgtaaaac
    22681 agaccagcat caagaaatct attcgcttgt tcaaagctat cttcatctgc cacgtatagc
    22741 taaggaaatc gaccagagtg atcaagaatg gatacgagaa gaagtaaagc agcatgctac
    22801 tttcagaaga ctccaagcct tatcgcaaga aacccaagcg acagctaaag aattaaaaac
    22861 ccgcttgatt gcttacagac aaggaaaact tgatgcacta tgtatccaga gcattcacaa
    22921 aagtaaaggt cttagttacc gaaatgtctt tttgattggt tgtaacgaag gaagtttgcc
    22981 ttataacgga gcgacagaaa agaaagtaat cgactttaat gaaataaaag cagaaccagt
    23041 aacgacgata gaagaagaac gaagactatt ttatgttgcg atgactagag caagaaatcg
    23101 tctgtatcta tgtgtacctc aaatgaaaaa ttcaagaaaa ttgaaagtat cgagatttat
    23161 caaagaaacc gggagtcgtg tgaaaaaagg aaagtgatag aagagagggt gtgacagaag
    23221 tggtcagttt caagaaagaa gaaggaatgt acgaaaattg cttctcaaat ttttgtgaaa
    23281 tttcagctta ttttcgaaga agctatttct ggaacacagt ttatt
//
LOCUS       DANKAY010000039        22989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000039 DANKAY010000000
VERSION     DANKAY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            164..280
                     /locus_tag="PWY98_001479"
     CDS             164..280
                     /locus_tag="PWY98_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2037236.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(335..2017)
                     /gene="recN"
                     /locus_tag="PWY98_001480"
     CDS             complement(335..2017)
                     /gene="recN"
                     /locus_tag="PWY98_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2037237.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2035..2484)
                     /locus_tag="PWY98_001481"
     CDS             complement(2035..2484)
                     /locus_tag="PWY98_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2037238.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2591..3409)
                     /locus_tag="PWY98_001482"
     CDS             complement(2591..3409)
                     /locus_tag="PWY98_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2037239.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3418..4308)
                     /locus_tag="PWY98_001483"
     CDS             complement(3418..4308)
                     /locus_tag="PWY98_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2037240.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4310..4534)
                     /locus_tag="PWY98_001484"
     CDS             complement(4310..4534)
                     /locus_tag="PWY98_001484"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2037241.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4539..5876)
                     /locus_tag="PWY98_001485"
     CDS             complement(4539..5876)
                     /locus_tag="PWY98_001485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2037242.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5877..6731)
                     /locus_tag="PWY98_001486"
     CDS             complement(5877..6731)
                     /locus_tag="PWY98_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2037243.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6828..7277)
                     /gene="nusB"
                     /locus_tag="PWY98_001487"
     CDS             complement(6828..7277)
                     /gene="nusB"
                     /locus_tag="PWY98_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2037244.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7270..7695)
                     /locus_tag="PWY98_001488"
     CDS             complement(7270..7695)
                     /locus_tag="PWY98_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2037245.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7941..9005)
                     /locus_tag="PWY98_001489"
     CDS             complement(7941..9005)
                     /locus_tag="PWY98_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2037246.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9177..9422)
                     /locus_tag="PWY98_001490"
     CDS             complement(9177..9422)
                     /locus_tag="PWY98_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2037247.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9561..9935)
                     /locus_tag="PWY98_001491"
     CDS             complement(9561..9935)
                     /locus_tag="PWY98_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2037248.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10018..10932)
                     /locus_tag="PWY98_001492"
     CDS             complement(10018..10932)
                     /locus_tag="PWY98_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2037249.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(10953..11756)
                     /locus_tag="PWY98_001493"
     CDS             complement(10953..11756)
                     /locus_tag="PWY98_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2037250.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11789..12778)
                     /gene="manX"
                     /locus_tag="PWY98_001494"
     CDS             complement(11789..12778)
                     /gene="manX"
                     /locus_tag="PWY98_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2037251.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12881..13372)
                     /locus_tag="PWY98_001495"
     CDS             complement(12881..13372)
                     /locus_tag="PWY98_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037252.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13563..16370)
                     /locus_tag="PWY98_001496"
     CDS             complement(13563..16370)
                     /locus_tag="PWY98_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2037253.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(16804..17097)
                     /gene="rpmA"
                     /locus_tag="PWY98_001497"
     CDS             complement(16804..17097)
                     /gene="rpmA"
                     /locus_tag="PWY98_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2037254.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(17110..17454)
                     /locus_tag="PWY98_001498"
     CDS             complement(17110..17454)
                     /locus_tag="PWY98_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2037255.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(17481..17789)
                     /gene="rplU"
                     /locus_tag="PWY98_001499"
     CDS             complement(17481..17789)
                     /gene="rplU"
                     /locus_tag="PWY98_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2037256.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(17809..17885)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            18071..18712
                     /locus_tag="PWY98_001500"
     CDS             18071..18712
                     /locus_tag="PWY98_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2037257.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(18774..20405)
                     /locus_tag="PWY98_001501"
     CDS             complement(18774..20405)
                     /locus_tag="PWY98_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2037258.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(20528..20887)
                     /locus_tag="PWY98_001502"
     CDS             complement(20528..20887)
                     /locus_tag="PWY98_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2037259.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21046..21119)
                     /locus_tag="PWY98_001503"
     tRNA            complement(21046..21119)
                     /locus_tag="PWY98_001503"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21083..21085),aa:Arg,seq:tct)
     gene            complement(21428..22111)
                     /locus_tag="PWY98_001504"
     CDS             complement(21428..22111)
                     /locus_tag="PWY98_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2037260.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(22239..22757)
                     /locus_tag="PWY98_001505"
     CDS             complement(22239..22757)
                     /locus_tag="PWY98_001505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2037261.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT         6620 a         5053 c         3810 g         7506 t
ORIGIN      
        1 aagggatgat gcctttatat ggctagttct attaacatta tttatttgta tcgtaactac
       61 ctattattta aaaaaaggga agtttttata aaaaagccct cttgtggaaa tggaaaaaag
      121 attgtacact tgaatcaact aattaatgac gaggttgaaa acaatgcgtg aaaaaaaaca
      181 aacaagtact tttgctaaag taacaaaatt tgtcatttgg acaatgctga ttttaacgat
      241 tggatctgtt gtattgactg ctgtaatgag tgtcatgtaa aaataaaata tagctatcag
      301 ctgctgaaaa ggacctgaac cactgcgagg tggctcaggt cctttttgcg agtttcaata
      361 gttcttttgc atgttcgagt gtgagttttg taatctcact tccagccagc atacgtgcaa
      421 tttcttcttc tctttctttt gttttcaact ctgaaacaga agtttgcgta cgtccatcaa
      481 tcactgcttt cacaatataa tattgatagt cggcgactgc agcaacttga ggcagatgag
      541 tgatgcacaa aacttgagag tatttcgata tttgagaaat tttatctgcg atggcctgtg
      601 caactcttcc gctcacacct gtatccactt catcaaaaat aatacttgtc acaccttgct
      661 cagaagaaaa aatagttttc aaagcaagca acatccgaga cagttcgcca ccagaagcga
      721 ctttcaccaa aggcttcagt ggctctcctg gattggtcgt aatgtaaaat tcgatttggt
      781 caaatccatc atgattcaat tgtctatttt cttcttttaa gaaacgtacc tcaaactcag
      841 tgttttccat atacagactt ttcaattcat gcagaatgga tttttccaat tcttttgcga
      901 tttttttacg cgcttcatga agatctgcgg cttgttgctg aagtagaagc tctttttgct
      961 ctagttcctc tttcattttg tccaattgac cttctgaaaa atcagaagaa gctagctctt
     1021 cggtgatttc gtcatagtaa gccaatattg actcaatgga ttcgccgtat ttacgtttca
     1081 actgtcggat taattctaaa cgttgcgtaa cttcttctaa acgactctca tctaactcca
     1141 ataaatcaat ctgacgactc atatcgccga ttgcatcttg cagcaaatag taagcacttt
     1201 ggatattttc ataaattgct tcatactcca catctagatg ggcgatccct tgcagttctg
     1261 aaacggccaa gccaacacca tctacactgt tttgttcatt attcgtcagt gctccgtaac
     1321 ctgcagctaa tccatcagca attttttgat aattgctaag tttttctctt tcttctttta
     1381 atttctcttc ttctccgact tgcaaatcag cagcagctat ttcttcttgc tggaaatgga
     1441 gcatatctat tctctgaaca taggattttt cattttgctg cacttttcgt accctttttt
     1501 ccagctcacg ataagagata tattcttgct gataactctt tttcttctgt tggaaagcta
     1561 catctccaaa acgatccaat aaagcaagat gtgattcagg ctggagcagt tcctgatgtt
     1621 cgttttgtcc ttggatatct actaaataac tgccgattct tctcagatta gctaatgtga
     1681 taatatgacc attcactcga caaacatttt ttccagttaa ggacatatcc cgtcgaacaa
     1741 tcagattgtc ctcatctgtt tcgatcccta attcaaccat cagttcagag aatccttctt
     1801 gttttggtag ttcaaacaac ccttcaagca cacatttttc tgctccttga cgtatataat
     1861 cgcttgaacc tcttcctcct gctaacagac ctaaagcatc gataataatg gatttacccg
     1921 caccagtctc tccggtcagc gcggtcattc cttcgtgaaa agaaagccgc aattccggaa
     1981 taatcgcaaa gttagtaata ctgatttctt gtagcatcac attcgctcct caacttaaag
     2041 gtaatgcagg aagtctttct tcaataaaac tgcctggtct tcagttcgtg taatcatcaa
     2101 aacattatcg tcatcattga taatcgaaaa taattctttc ttgtaacgtt tatcaatcaa
     2161 atttgctgct gccgaagcat ttcccggcaa tgttttcaaa ataacaaatt tttccatttg
     2221 gtcaactgcc acgaaagcgt cttttaatag tttttccaat ttagtattta catcttcgtt
     2281 tgtttcagct ggtatgctat aacggtagcc gccatctgaa gccggtacct tgatcagttt
     2341 cagttccttt atatctctgg aaatcgtagc ttgagtaaca gatattcctt tgcttttcag
     2401 gatttcaaca aattcttcct gctttcggat atcttgctca ctcaacaatc gagtaatcaa
     2461 acgatgccga tctttttttc tcattacgtc ttcgctcctt agcatattct tcttaatcaa
     2521 tgatagctta tttccttggt ataataaaga agaatccgca tacttctcac ttgattttca
     2581 cagttacaat ttatttatgg tcaaactgtt cgtgtgcatt tgaaaccact tctgccatat
     2641 taatagctga attgattgtt ccgctttcgg gtactttcct caagtgagcc aagaactcga
     2701 tatttccttc tccccccgtg atcggagaat aacttaattc cagcacatca tatccacctt
     2761 gtgccgcaaa tgacaaaatg tcttctaaaa ctttttgatg ggtctgaggg tcacgaacaa
     2821 ttccgttttt ccctacagct tctctgcctg cttcgaattg gggtttgatt aaagcaatca
     2881 cttctccctc ttcttttaaa atagcatgta atggaggcag catcagtttt agtgaaataa
     2941 aagaaacatc gataacagca acttctggaa ttcctaaggt aaagtcttcg ggttttgcat
     3001 aacgaaaatt cactctttcc attacgacga ctctaggatc ttgtcttatt ttccaagcta
     3061 gctgattgta gccaacgtct aaagcataac tcatttttgc gccattttgc aaagctgcat
     3121 cagtaaatcc gccagtggat gcaccaatat ctaacaatgt ttttccttca acagatagat
     3181 caaatgcttt gagtgctttt tctaatttta atccccctcg agaaacataa ggaagttttt
     3241 tacctttgac tcttaattca ctactcactg gaattttttc tcccggttta tcaaaccgct
     3301 cgtttttttc gttgtaaatc aaaccagcca tcactgaacg tttggcttgt tctcttgttt
     3361 caaataatcc ttgtttgacc gctaatacat ctacgcgttc tttttccatt ctttccctca
     3421 tttctccaca tcaaattgct tgatcatctg gcgcatcagc ccgccatcaa aagaaaaaac
     3481 gtcctcttct aatttgtcta tgatcttgtt ggcggataac agctcaatct ccaacgcctc
     3541 acgtgtcttt tctaggccta ataaacgcgg gtaagtattt ttttccatac gttcatcttt
     3601 accagctgtt ttccctaatg ttttcgttgt accaatcaca tcgagtagat catctcttat
     3661 ctgaaaagct aaacctaaat gttcggctaa tcgttgaagt agcaataaga tttcttcagg
     3721 ttgcttcgct aatatgccac cagccagcaa ggcataacga atcaaatgcc ctgtctttct
     3781 ttcatgaata aatgctaatt cttctaaaga aagctttttc gattcgccct cgatatctgc
     3841 agcttgccca gcaaccatac cttgtgatcc cgcacaaaca gctaattgct gaagcagcag
     3901 aagcttagga gaattaccta agtgtgccat actgattagc tggaatgctc ctgtaagcaa
     3961 accatctccg gccaatatag ctaatgcttc gccgaatact ttatgattgg taggttttcc
     4021 tctacgaaga tcatcattgt ccatcgcagg caaatcatcg tggatcaagg aatacgtatg
     4081 caccatttct aaagcagctg ctacttgata gacgggaaca tcaataggct catcaaagga
     4141 agcaacggcc gttaaaagta ataagggacg tattcttttc ccgcctgctt tcactgaata
     4201 actcatcgca tcatagagtc ctctatccgt tgtatgctca tctaaaaaag acaagatttc
     4261 tttttctaca gcaggtagat gaatagctga aaagtcagtt aatctcatct cagctttcct
     4321 cgtttccgtc aaaatcaact tcttcatttg attctgtcat cattttcgtc aaggtctctt
     4381 ctgctttagc taatgtatct tggcaatgtt tgcttaattc cattcctcgt ttaaacgaat
     4441 ctagagctga ttctaatgga acgtctcctt gttctaactg catgacaatt ttttctaatt
     4501 cttgcagtga ctcttcaaaa gttggatttt ccatattttt attctccttt gtctatctct
     4561 tgtacttgtc ctttaacgat tccatcttga taatggatcg tcagcaaatc cccttgatga
     4621 atctcggtga cactttttac tacttgatca tttaacgtcg tatagctgta gcctcttccc
     4681 atgattttca atggactcaa caaatcaaga gattgaacag cttgagcaaa acgctgctgc
     4741 tgttgtttca tatattgttg gattttctct tctaagcgtt tttctagata agcagtttct
     4801 tggcgagctg ctttaactcg ataaagcgga gccacttgtt ccaattggtg gcttaatgcg
     4861 atcgtttgtt tttctttcgc atgataaatt tgctgtgtag cttgatataa tcgctgtttc
     4921 aactggtcta atttgatggt ctgaccttca tacaatcttt caggctgccg aaatacatag
     4981 gaacgtcgag cacgctcgaa ccgttcttgt ttgcgctgga tctggcgtac aaacgcctgc
     5041 tgcaatctag cttgccgttc ttgaatgcgc atcaactctt ctgacaaaac aggaacagct
     5101 aattctgcag ctgctgttgg agtggcagcc cgaacatctg ctactaaatc agcgatcgtg
     5161 gtatctgttt catgtcctac agatgaaatc accggcgtat tagcttgata aatcgcccga
     5221 gcaacccgct cttcattaaa cggccataaa tcctcaatcg aaccaccccc acgaccgatg
     5281 atcattgtat cgaaattgcc catctcttct actctctgga tattcctgac aacattgtcc
     5341 gctgcttggt ttccttgtac aactgttggg aacaaaacca attggacaat tggatatctt
     5401 cttttcaccg tcgtgatgat atcgcggata accgctccgc ttggactggt aaggaccgcg
     5461 attcgctttg ggaaacgtgg cagcattttt ttaggccctt gaaaaaggcc ttccttcatc
     5521 agcttttctt ttcgttcttc caattcttga tataaagcac ctattccatc aggctccatg
     5581 tgctctacat agatttgata agaacctccc gcttcatata atgaaatacg cccaacgaca
     5641 aggactttca ttccttcttt tggctggaat cgtaattttt ggaatgctcc tttgaacata
     5701 atagcagata tttttgcatg gtcatctttt aagctgaaat attgatgggc attggcacgt
     5761 aaacggaaat tcgatatttc gcctgttaaa tatacgcgtt ctaaataagg atcggcatca
     5821 aattttcttt tcaaatactt cgttaatgta gtgacagtta aatattcttg tgccatctat
     5881 tcacctgctc caaaagcttc ggcagatttt acggtttgat acatcagcat cgtgatagtc
     5941 attggcccta caccctttgg cacaggagtg atccagcctg caataggctc cacttcatca
     6001 aatttcacat caccgatcag cttaccgtta gcatcacgat tcatccctac atctatcact
     6061 acagcacctg gttttacgaa ttcttttgtg acaaaatgtc cacgtcctat tgctacgact
     6121 aaaatatctg cttcttgtgc cagttcagcc agattttcgg ttttagaatg agcaacagta
     6181 acagttgcat ctgccatcaa gagtagctga gccatcggtt taccgacgat attgcttcgt
     6241 ccgataacta ctgctcgttt acccgccgga tcgatagaat aagcttcaaa cattttcatg
     6301 atgccgtatg gagtacaagg aatcttgtcc ggtttacccg caaggagtct gcctaaattc
     6361 atcggatgaa aaccgtctac gtctttttca gggcggatag ctaacaggat cttttcttca
     6421 tctatatgct ttggcaatgg taattggacc aatataccat gaaaactagg atcttgattg
     6481 tatttttcaa tttctgcaag tagttcgtct tcagaaatcg tctcaggata acgctctacc
     6541 aatgaatgga ttccgatttc ttttgctaca cgttctttat ttcttacata gatttggctg
     6601 gctggatttt cccctaccaa taaaacgact aatccgggat ggataccatt atctttcaat
     6661 ttttggattt tttcagcaat ctctgcctgc attttttcag ctaattcttt tccgttaatc
     6721 aattctgcca tctaaccact cccagatatt ttttgtgttt tcattctacc atagaatttt
     6781 cccgatttat aacaggttta tgatgattca tagaaaaaag ctaggactta cgccccagct
     6841 tctaattctt tcaatacatt ggataaaaca ccgttgacga atttacgtga acggtcatca
     6901 ctaaatgttt ttgccaactc gattgcttca tttaaagcaa cagccgctgg tacatctgtc
     6961 acataagtca tttcaaaaat cgcgatacgc aagatcacca aatccatttt tgagatccga
     7021 ttgatgtgcc aattgttttt taaatgtttt tcaatcactt tatccaattc atcttttttt
     7081 gcacagacac cgccaactag cgtatcaaga taaacaggaa caaactctga ttcatcttcg
     7141 ttgatcatgt cgcggtggtc taattcgatt gcgttgaaaa tcgcatcttc ttttgttagg
     7201 tctgcattga agtctaatgg aaataaagcc tgcaatgcca tttcccgaat ctcgtgtctt
     7261 gataattctt tattcattct cttcctcttc gtcttcaaaa agttcttcga ttgttggttg
     7321 ttctaatttt tcaggaacaa cagctactac atggatattg acttcagata aatcgatatc
     7381 cgtcatgaaa aggacttgct ggctgacccg ttcctgcatt tccattgcta ctttaggaac
     7441 tgaaacacca tattctagat aacagtaaag atctactttc aagccatctt cctcgtttgc
     7501 taagtaaaca ccttttccgt gagctgcacg tcctaataat tcagtgacat tgctggcaaa
     7561 agttccgcgc attccgtata cgccttctac tttagaagct gcaatcccga taattacttc
     7621 gatgacttca ggagcgatca cgatctctcc taaatcttgg tttgcgttta aaatcaaatt
     7681 tttttcatct gccatttatt tattcctccc tttgcctaag cacgagatac atccgaattc
     7741 tctatgatgc ggcatagtga tcacgtggta agaaaactgc cgccgatcag ctgcaaactg
     7801 ctatgaattg gagaaaacac ccaactttat tatctcttac agcttaaaag ttcttctata
     7861 gtgtatcatt aaatcgagtg attttctata acaaagtctg tgaacactga tttttacgct
     7921 tacttcttct attgatgaaa ctataagatg atcaattctt ttggtgaacg agtcaacaca
     7981 cgatttccat cttctgtgat caacaagtca tcctcaatac gtactccacc gattcctggt
     8041 aagtagatcc ctggttcatc agtgatcaca tttcctggta caaattgctt ttctgcacga
     8101 gaagaaacgt ttggtccttc gtggatctct aaaccgatcc catgtcctgt agaatgtcca
     8161 aatgcttccc cgtaaccatg ttttgaaata taatctctag caactgcatc taattggaca
     8221 ccagtcattc ctggttttgc agcatcaata actgctaatt gagcatctaa gactatttga
     8281 taaatttctt ttaatttttc cccaggatca ccaatagcga atgtacgtgt catatcggaa
     8341 acgtaccctt cgtaatagca accaaagtcc aatgtgatca agtcaccttg ttcgatgatt
     8401 tttttgcttg ctacaccatg cggcatagcc gaacgcaatc cgctagcaac gatcgtatca
     8461 aaagaaacac ctgaagcgcc aagagaacgc atataaaagt ctagttgatt tgcgacttca
     8521 atctcagtca tgcccggttg gatcattttc aggatgtgat catatgccat atccgcaata
     8581 tgacatgctt tttcgataat agcgatttct tcttcatctt tcacttctct gagttcttcg
     8641 atcatctgtg aaacaggtac taattccgca tcgatgattt cttctaaaac cgaatattct
     8701 aaaaatgaga ctgttgtttc ctcaaaagct aatgagtgca gttcttcttt gcgtactaag
     8761 tttgctacct catcaaaaat aggtccgaca tttttaataa tttcaaaccc ttgagcctgc
     8821 gctgccgcct gctcggtata acgaaaatca gtaataaaaa atgctttttc caaagtaata
     8881 acagctaatc ctgtcgtacc tgtaaagttc gttaagtaac gaagattgta cggactggta
     8941 accaaaaatg aatccaaatt ttcttcttgc atttttttgc gcaatttttc tacacgtagc
     9001 atcataatga aactccctct ttctttgcac gcttttttcc attatagcaa acttttcttc
     9061 gtacacgaca gttaaataaa aatttccgga aaacttcttt ttattttaaa taaataaagc
     9121 caaaaaggag tcacagtctc gctgcaactc cttttagttt actattttgt aaaaacttat
     9181 gatcgttttc caaagaaaaa tgagacaact gctaccaaaa tcactgcgcc taaaatagat
     9241 ggaataagtg ccatacctgc agcttttggt ccccattcgc cgaaaatcgc ttgtccaata
     9301 cttgaaccga ctagtccagc aatgatattc gttacacagc ccatcgaatt tcctttactg
     9361 gtgatcgcac ctgcaattgc tccgatcacg gctccaacaa tcaatgtcca taaccaactc
     9421 ataatataac ctccttcttt gctaatcaat tttagcatat gggatttaaa ggggacaaat
     9481 aattggtttt gctatttcat attttcttct gcaaagtaaa aaaataaaga aaacctcctc
     9541 ttagaagttt tctttatttt ttacttgcgt ttaaacatac tacgaatacc acgtttcact
     9601 acatcaaaga aactcaatga ttgtaccatt ctatcttcat ctacatattc tctgatcgca
     9661 cgaagcactt ttttaggttc tttggaagaa aatgtgtatg taccattttt tttcgtttga
     9721 atggcatatc gaggaatcca ttttcccttt agcatgactg atgctagtac ataatccact
     9781 tcttcccatg gaatttggat atatttgcgt ctatctcgcg catggtaaaa ctcaaaacca
     9841 ttattaccta tcatgatttg cccgtaatct gtcagacccg taaacgaagt agcatcaatt
     9901 accaagtcta cttttgtatt gattgattga accattttct ccctccagat atttgtttat
     9961 gaacagtata ctatataaaa aaagctcggg acaacgcccg agctatattt ttatggatta
    10021 taaaagtccg atgacgtgtc caacaacacc gactacgaat aaaccaagga tgataacgat
    10081 tggagaaacc tttttcttaa gcaaccacat acataacaat gttaatccta atgcagctaa
    10141 accaggaatc aattggtcta agttgtcttg taatgttgtt accttttcag gagaaagggc
    10201 taagccatta ttaacttgtt caaatgcact cttgattcct tcaccacctg aaggcaattt
    10261 atcccattcg atgtaagcac ctttatctaa ttttacagtt gatacaactg gtaagaattt
    10321 gattgatacc catctttgta caagggcagc taatacgaac atccctaaaa ttgaagcacc
    10381 ttttgttacg tcttgtaaca atccgccaga aaggtcatca gtaattttag aaccagcttt
    10441 gtagccaaat tcttgtgtat accacatgaa tgcccaacga ataatgttcc aacctaagaa
    10501 gaagatgatt ggtccaagga tatttcctcc catagctaat gaagcaccta gtgcacctag
    10561 cattggacgt actgtaaacc agaacactgg gtcaccaaca ccagctaaag gacccatcat
    10621 acctacttta accccttgaa tcgctacatc atcaacaggc gcgccattcg cacgttcttc
    10681 ttccagagcc aatgtaaccc ctaaaattgg agaagcaatg tatgggtgtg tattaaagaa
    10741 ttccaagtga cgttttaatg ctgcagaacg ttcttctttt gttttgtata attttttgat
    10801 tgctggaatc atagaaaatg cccagccacc gttttgcata cgttcgtagt tccatgaacc
    10861 ttgaatgaag gtagagcgcc acgcaacagc taaacgatct ttttttgata atttgatttt
    10921 ttcttctgcc attttatttt cctccttcaa gattaatagt cattcaaaat gtcgcctaaa
    10981 gggtcaccag agttgcttcc gcctccatta tttgatgaac cacccatttt agaaaggttc
    11041 aagtaaataa gagctaatgc aacacctaaa gcacctagag caatcaatgt taattgagaa
    11101 attgcagcta caacgaaacc aataacgaag aatggccata cttcttttgt agccatcatg
    11161 ttgattacca aagcgtaacc tacagctacg accattcccc caccgatagc cataccatct
    11221 gttaaccatg ctggcattga ttcaaggaat gattgcacag tttgtgctgg gataaatagc
    11281 aacgctgctg ctgggaccgc gatacgaacc ccttgtaaac atacagcaat gatgtgccac
    11341 atttcaacct tacggatgtt tccttcttca gcagccgcat ccatcaaatg aacgattgga
    11401 acagcgattg tacgtacgat cattgttaag aacaaccctg caacagccaa aggaacggca
    11461 atcgcgatcg cagaaggaac acctttaaca ccttgtccac ctaaaactaa aataattgct
    11521 gatgctactg atgctaatgc agcatctggt gctacagcgg caccgatatt tgcccaacct
    11581 aaagcgatca tctgaagcgt accgccaagt acgataccag cttcgaggtt accggtaact
    11641 aaaccgatca atgtacatgc cactagtggt tgatgaaatt gaaattcatc aagaatacct
    11701 tccataccag ctagaaaggc aactaatacg actaaaatta ttgaaataat agacatgata
    11761 agcctcctat aaattttaat attatttatt acgcttttgc taattcagct tttgcttttt
    11821 tcaaaatatc atccatgtta tcgcgtgaat cattaggtac tttacgtaca tcgaatttca
    11881 cacctttttg ttcaagcttc tcaaacgctt caacgtcttc ttgtcccata gacaatactt
    11941 tacttacaac gactttaccg accgaatgcg ccattgaccc aacatttaat tctttgatgt
    12001 ctacgccacc ttcaacagct gtcaatacgt cttcaggatt ttcaaataaa agcaatgctt
    12061 tggtatttcc aaaacgaggg tcttttgcta cttcgatcat tttgctaata ggaataacat
    12121 ttgcttttac acctggagga gcagcttgtt cgattagtct cttacgcaga tcatcctttg
    12181 atactgcatc tgaaacaacg ataatacggt ttggttgcac tgcttttgtc caagctgtcg
    12241 caacttgtcc atgtaacaga cgtgagtcaa cacgtgctaa tacatatttg atttttccgt
    12301 ctccgaccac agttccttct ggaagtgctc ctttaggttg tgcgtcttca actgctgctt
    12361 tcggtgcttc tgccggttct aattcttccg gttttacttt tacaccgtct tttgctgttt
    12421 caatgatgtg tgctgcaatt tcatgagcag attccattga aaagcgtgat gcgtaggctt
    12481 caatcaacat tggtaaattc aaaccgctga cgattgccca tttatctttg tgctcttcga
    12541 ataatgtgtt tgattgattg aacggtgttc cgccccataa atcaactaag aataacactt
    12601 cgtcttgatt atcaaaagaa gcaaccgctt cttccaattt ggctctcaag tcatctggac
    12661 cttcacttgg tttcaaaatc acggctttga cgttttcttg ttcgccaaag atcattgaac
    12721 cggattgtaa gattccttca gcaaattgtc catgacttgc taggataatc cctaccatat
    12781 ttatacctcc tatttacttt tgttgtaaca aattcaaaaa tattaataat tgctaaccat
    12841 tacaacagaa ttatttgacc ttttccaaca accattcttg ctacaccttt ttgattttcg
    12901 ctagtagttc catcaaaagg attttcctgt cagcaggtac ttttcgaatc tctaactcca
    12961 cttcttgact gttcaagtaa cgaaaggaat caatatcttg ttgatttact gaaacaaaat
    13021 tggtaatcat tgatttgcct tcagtaaaac tcataccgcc gatattcaac gacttcaact
    13081 gtacaccgca gcggacaacc gcttcaacat cttggggatt ggtgaataaa agcatcactt
    13141 ttgctcgtga taataatcca ctatgataga tttgaatcat tttctgcacg gttattacat
    13201 ttgctgtgat ccccggcggt gcagcttctt ttagtagaaa ctttctcaat ttatcttgag
    13261 cgactacatc gctgatcaca atgatacgtt caacacctgt ttgcttcgac caaactgttg
    13321 cgacttgccc gtggattaaa cgatcatcta tacgtgccaa tcgaatatcc atctgcaaaa
    13381 ctccttcttc ctcactactt cacagaaacc cctatcattt ctgtgtgaac aaccacctcc
    13441 tagcacacac tactataagc aaaaagcgtg ccaacttttt tacgatacac taattgaatt
    13501 taaaggattc ttaaccaata cacaaaaacg atacactata aaagtgtatc gtttttgtgt
    13561 attcatggca cagtgtattc ttttttatct aactgattat ggactagacg tagcagataa
    13621 tacatttctt ctatcggcac ttttactttt atctgatctt ccaacagaac aactgaggct
    13681 gccaattggc tataaagagg atcattcgtt gtatttgtca attcgttttc ttccacagtc
    13741 aatggttcat tccgaataat acgttcgatc attcctgctg tgtgcatcac taggttgatt
    13801 ttgaatccat attcttcatc gccgattccc gtcgtctgag agatttgtgt agaaaattgc
    13861 cacattggat cgattaactt actaccatta ataaagatga agttatcaga gataaactca
    13921 acacacgtat ttttagctga ctcttcatct agggagatga attcagtttc ctccaattct
    13981 gattccatca gcaactgatc caaaatgact tcgatatttt gatcaataaa acgttctaaa
    14041 ggaataaatg gggcttggat attcaaatca gtaatcccag tagtagctat gatctgatat
    14101 tctttttgga taactggcag ttcttctttc acttccacaa tcgacaaagc tagaacttct
    14161 aattctgttt cttgacgctt gtttaccgct cgttcgatca attctttgat tctttgtgct
    14221 gtaccttcgc cagatgcgca gatagccaaa acagcttttt tcttatgaat aggttttgct
    14281 tcctgaacag attctacaga agatatttct gcgtaacctc gaaaattttt caatgattca
    14341 tgcaacatat ctagatctgt ccctaatact gagacctttc gtacagtttc caaaactaga
    14401 gaagttgtta ccatatcaac agtacgtact ggaattccag tgtctctttg aatttgcgaa
    14461 ttaaacgacg caagggaccc catatctact aagagtatga ccccgcttcc ttcatctact
    14521 tcttgtacat tgcgttcaat tcggtccaat gctgttttcg gatccatatc caaaggcatg
    14581 tctactgctc gtacgttctc tgcatctaac aactgttgga caacttggac catactgctt
    14641 gctgtgctgt ttccgtgagc tgcaacgact acaccgattc tgccgcttgc ttgatttgcc
    14701 cgtaacgaga cgagtagaac ggtcaagtaa tagtcttcac tttctggaat ctccacttga
    14761 aaatacgttt cgatataact acgcacttct ttcgcaacag catattcatc tggaaaatca
    14821 aaagccatct cacggatgtt atcattcgta tgccgttctt ctcctaagtt gatttttttc
    14881 aaaaaagaac tgatatgcaa gctcatagca taaataaagt tttgctgaaa ctcatatggt
    14941 aaagatcttt tgaccattac ataaatctga ttggtcactt ctatgatttt cggatctaca
    15001 aattctgcta atttgctgtc tgcattaaaa gaaaaaccat ggtctttata gaaagacttc
    15061 agatggatgt tgatatctgt agaaatgaaa tgattgattg cttcttgatc caaaccatcc
    15121 gacttcagta aagctgcttt atctccgatg atatcatata gattataagg taactcatag
    15181 gagtccgatt ggattttcat gatcgtatca tttggtgcta cagttatctt tggctccaat
    15241 attttagaaa gctctgccat tgcactgcgg ttgcttgcta attgaatcaa tccgctacga
    15301 atcccttctg tcaaatcatc gattgtgatc gagatttcgg gtgattgcat atgatttaga
    15361 aatcctcgtg cacatactaa ctggatattt gacttcaatt gtcctatatt tccataggtc
    15421 acactgccga tcaatgcttt tgctacatct tctgtcaaag aaatctttcg ctgcgtgcgg
    15481 tttgcttcgt gggcaatcat gatttttact aaatcgactt tctcatttgc aggacgtttt
    15541 tcaaaagccg gaagtttgat attgattggg atacgccgaa caaaagtttc taatagagaa
    15601 gaacctgggt cttctgtggt cgcacctacg atccgtacat ttgcttcatg ggatttcgtt
    15661 gtttccccaa gacgactgta agttccatga tccataaagt aaaagatcat ttcttgacct
    15721 tcaggcggca accggtgaat ctcatccaaa aacagcatac cgccatccgc ttgatcgata
    15781 atccccattt tttcttcatc tgcgcctgta aaagcacctt ttgcgtaccc aaataaatga
    15841 ctcatcaaaa gctcagggtt attggcataa tcagcacaat tgaaaacaat cagttccgtt
    15901 tcctcatcta ttacatgatg cgcttttgca aaatgataca tcgcgtgtgc aaaataagtc
    15961 tttcctgatc cggtaggtcc tgtaatcaaa caatttaatc ctcttggcgg atacaaaatc
    16021 gcagcttttg cctgttccac tgaatttttc atgctgccgt tagctccaat gatttttgca
    16081 aagatatcct tagtatctat ttggagtgta tttttttctt cagagaccgg cgtttctttg
    16141 taactgacta catgttttgt aactggcttt acgtgcgtaa cgagtgtttg atatatgtaa
    16201 cgtactggtc gtccgtctgt ctttatcagt tttccttcgc gcactagctg attcagatct
    16261 ttactgctat ttgttcgctg aatccctaac acttcagcaa tttcctgtgt tgttactcct
    16321 tgatcatatt ctgcctttgt taaatgagcg gttttttctt tgacatacat ataaattcgg
    16381 tcaatgcgtt ttgccaatgg tatcgctcct tttctataga ctattgtact taggcttcaa
    16441 aacataatac tatgtattta tttctgtttc gtcaactatt tacctgattt tgttttgttg
    16501 ataacacgcg gatagataga aatgttcaat ttaaaagaac gaaaaatccc gatacgattt
    16561 ttataatgga aacaagaaaa agaacactgt ttaccatata attatttcac tgatagcaaa
    16621 caatatagct tccatcaatt gaaaacgata gttatcaaaa catcctgatt ccaaattggc
    16681 aaaaaaatat taagtgtaaa aagacaaagt aaacttcgta gcctccacta caacttttat
    16741 ttttataaac aaaaaaggct gtgacaaaag tcttgtcaca acctctttaa tcataagatt
    16801 caattaagct tcttgagcta ctggataaac agacacttgt tttttgttac ggcctttacg
    16861 ttcgaaacgt actacgccat caacttttgc aaataaagtg tcgtctccac cgatacccac
    16921 gtttacacct ggatagattt ttgtaccgcg ttgacggtaa agaattgatc cacctgtaac
    16981 tgtttgtcca tcagcacgtt tagcacctag acgttttgat tctgagtcac ggccgttaga
    17041 tgtagaaccg ccaccttttt tgtgggcaaa taattgtaga ttcatagata acaacatggt
    17101 ctgcacctcc tatttatcat taattgtttt aatttggata tattctgagt tttcagcttc
    17161 tatcgattgt aatcctaata aaagattttc taatagaatc tcagcaatat tgttttgttc
    17221 ttgtgtcatt cctgaagtga cttcggtata aagataaccg ccttcttctt cattcatttc
    17281 aacaatcggc tcaaaaccgg ctaatgaggc aatcccgttg acagtgctga tcgctaacgc
    17341 agaaacaccc gcacaaacga tatcacttcc ataaggacca gcatcagcat gcccggtcaa
    17401 agtgaatgaa acgatctgac ctgcgtcatt tcgcttaaat gttcctttga tcatatatag
    17461 gccttctttc tcaaacgcta ttaagcgttg atcgcgtcga taaccacttt tgtatatggt
    17521 tgacgatgac cttgtttacg gtgtgaatgt tttttaggtt tgtatttgaa agtaacgact
    17581 ttcttttgtt tgccgtgttt ttcaacagta ccttcaacag ttgcacctgc aacagttgga
    17641 gcgcctactt ttgtagattc tccgcctact aagataactt cgtcaaaaac aactttttct
    17701 cctgcttcaa cgtcaagttt ttcaacatag attgcttgtc ccacttcaac ttttacttgt
    17761 ttaccaccag ttttaataat tgcgtacatg ctataaagca cctccttctg atatacttag
    17821 actcgccatc cgaagtgaca gcatatgctg tgcttattac ttgttctgag cggttgtagc
    17881 tgtggagtcc acaattacaa cagtactagc ttaccagaac agcaaccgtt agtcaactaa
    17941 atttaaaatc aaaaagtaaa acaaaaattc aagcaatact tctgcttttc tatcctcaca
    18001 gttctaccaa ataaaatagt cgcattgtgt ttcttttatt cttataataa aagaaaaagg
    18061 aggagcataa atggaatacc caaaagaact gatacaagaa gcttctttaa gaatgaacgg
    18121 ccgtcctgta gaaggattta tcgctgggca aggacctctt catcctaaac tgatgctcgt
    18181 gggagaagcc ccagggaaaa cagagattga tacgcatgtt ccttttagcg gtcaagctgg
    18241 aaaagaattg atgcaggcac tttcttcaac tggcttaaca cgagaagagg tgtatatcac
    18301 cagtgctgta aggagccgcc cgtatcgcgt gactcatcgg atcaataaac ggacacaaca
    18361 gtcagaaact gtttatccaa atcggacacc tactcgttcc gaagtatttg cgcatgcacc
    18421 aatattggat tacgaactga tgaaggtcca gccaaaatta attgcgacac ttggaaacat
    18481 tggtcttcaa cgtttacttg gcaaagaata tacggttacc aaaaatcatg gtcaactcta
    18541 tacagggcct gtcttacaat taacagaaca aaaggatgcc tacgaatggt cagaaaaaaa
    18601 ctatcgaatt cttccacttt ttcatccagc agctattttc tacaatcgct cccttgtacc
    18661 tgttatccat caagactggc aagtactagc ttcactttta cagcaatatt aaaaagaaca
    18721 gacaagacca taaaatcttc agtttacagt cttgtctgtt ctttttcatt gttttaatgg
    18781 ctaattgcag tatatttcgc atgtaaagct gtcaacgcat gagcaactgt ttgatgttga
    18841 tgcgtgaatg tatgttccca caagtgagaa agttgttttt ctacttctgg ctgcaaggaa
    18901 gtatagataa tcgtttgtcc tctttgctct gctttttctt gaaattcatc aaaaactgca
    18961 cgtgccgtca aatcaatact tggtacgcct cgcatggaga aaatgacacc atcgtgatga
    19021 tttagttctt ccaatttatt tttgagtttt tctgaagaca taaaaaacag tggaccacta
    19081 atatacacta ctgtccagtt attcagcttt tccgtttctg gcagccccat tctttgccaa
    19141 tcgatcgttt ctaatgaaat cgtgatagca gcgcttttga caacaaaaaa tacacagccg
    19201 ccgatcaccc cgatcacgat agcaatactt aaatcaaaga taactgtaca aatcatcgta
    19261 aggataaaca atagaactgc tgaccaatat ttattagtaa atagctcctt gatcgtttcc
    19321 cattcattca tacgccaagc cgtcacaatc aaaacacctg caagtgctgg cattggaatc
    19381 tgtgacatca ctggcgcaaa aacaagcatg gagagcagta agacaactgc atgtatcata
    19441 ccagctaatc tagtttgtgc accagatttg atggccacgc ttgtacgggc gatggccgca
    19501 gtagcaggaa tcccgccaaa gaaaggaagc aacagatttc caattccttg cgccattaat
    19561 tcttgattgc tatctaaggg cttatttgcc attcggccag ccgaagcacc acatagcaag
    19621 ctttcaatca ttcctaataa agcaatgcta atagctggaa ctgccacttg ttgcaaggca
    19681 gaaaagctaa aatctcccat attcaaacga ttactactga tcaatgtttg tggaatctta
    19741 cccacagttg ctactggtaa atggaacaat agcataactg ctgttgccaa aatgatagcc
    19801 agcagcgaac taggcattcg tttcgcccat ttttttggat agataaacat ccctaaaatg
    19861 actagccctc ccatgatcaa agaggctgtg tttatcggaa atcctatcgt ttgataacta
    19921 cttagttttt caataacgtt tgtaccttcc gagtgaacac caaataaatt atcgatctga
    19981 cctaatgcaa taatgatagc aatacctgaa gtaaaccccg taattacggg tgctgggata
    20041 aaagaagtca gtgttcctaa tcggaaaagt cctgctgcaa gtaaaaatag acccgctaaa
    20101 aaagtagcaa gcaatacacc ttgcattccc tttgttgccg caatagacat caagatagct
    20161 gccatcgcac ctgtcggacc tgatatttga taaaaaccgc ctgataaacc gccaataacg
    20221 ataccggcaa tgatagccgt aatcaaacct gcggccgcat cagctccgct tgatacccca
    20281 aaagccaagg ctaaaggcaa tgctacagca gctactgtga tcccagccaa aagatctttc
    20341 tgaaatcttg aaagatcata acctgaaaat tcttttttta gtgaggaaac ataatttttg
    20401 aacacctatc taactccttt ttcatgaaat tttctaaaat accattcatt agaatatcat
    20461 atttcatgag ataaagtaat atttttcaca aagtttcctg ttagataaat tcttattttt
    20521 tctatattta taagaaaata gaaagcaatg ccaacagagc tagcccacct tgtttgagta
    20581 aaatccaacg atcgctagtc aaactgccat ataatgcaac aaaaaaaata gatattaata
    20641 gcatacttgt gatttcttta gatgcggaag aaagataagc tccataaagt agtcccactc
    20701 ctaatagtcc attgtacact ccttggttct taaacaatgt ttgaacagat tttctgctta
    20761 actcttcttt actcatttta aaaaccctac ttgtcgtgtc agaatcagtt ctcaccgttt
    20821 cgatatacat gatataaaaa aattcaattg caactagagt cactaaaatt ctagatagta
    20881 tcgtcatatc ttcttcctct tttctgatga gttttatttt tttgctcctt ataattgtac
    20941 tcgataagga tgttttttta ttttatttgc ctttgaaaac aaacaatatt ggcacaaaaa
    21001 acaaagaaaa accgttagaa acctcagttt ctaacggttt tttagcgtca caggagggat
    21061 tcgaaccccc gaccgtccgc ttagaaggcg gatgctctat ccagctgagc tactatgaca
    21121 agaatcagcg gaacatcaat aattatagat aattaaattg cttctgtcaa tacgaaatag
    21181 ttatttaatt agcaaataat gaattatttt ggcccaacta ctaaatcaat cttttaaaac
    21241 gagacattct ccttcagctg ttctaaggtt atcaattgaa aacaggagct acattgactt
    21301 catgctactc ctgtttattt agatttcctg tatcctttaa ttgacttaca aatatagaaa
    21361 cttttacatc aagttcgaat tgagccctcg attcaatcga ttatctacag gctgccataa
    21421 tcatgtatca caactcgatt gtttctgatt cctttgtcat taagatcaca ccatcaccta
    21481 atggcaagat tgtcgatgtc aaatcgggat gatccatcac aacacgaaga aattcattta
    21541 atttacgatg gatagctcgt ttgccacggg gaatctcttc atctgggagc aagatcgttc
    21601 ctccttggaa gatgtcatct accatcaaaa cgccaccttt tcttaacaaa cgtaagcatt
    21661 caggtaaaaa ttcaatatat tttgattttg cactatccat aaaaataaag tcatacggac
    21721 cagacaactc cggcaagata tctgctgctt gaccttcaag caaagtcact ttatctgtca
    21781 gtcctagacg ttcatagttt tttttagctt tctcgatcat gacatcgaaa cgatcaattg
    21841 tcgtaacatg agcgttttct ccgattgtcg tggccatgac acttgatgaa aaaccgatag
    21901 ctgctccgat ctccaagatt cgttcaggct gaagctgtcc aagtaaaaat ttcaaaaata
    21961 caactgtttc atggggaata actggtactt ctgctacatg agcttcttct tccagtttgc
    22021 ctagctcacc cggtaatttt ttttgctggg tacgcataaa atccaatagt tcttctttga
    22081 ctacaggacg atgcatcatt tcattgcgca tactactcct cctcttcaca cattatacga
    22141 acaactgact aaatctacaa gctgcatgag tgttttctcg tgtttcatgt tcagaagagt
    22201 cgttggatct ctggaataca tttatgtttt taacattctt acaaaaattt ctgtgttcgt
    22261 ctccgcttct ttttcaactc tacaaaggta ttccatgctg tgattcctgt taggcaaaaa
    22321 gcaatcgtaa agaataaccc ctgaatccat gcaaatgccg tttttgaaag gactagtcca
    22381 atatatggaa tctgaaagat gggactaccg ataatatttc tcttttttac tactccaggc
    22441 tcaatataag tctttgtatc agcttttgta taaaaatctt cgtggttgtt ttcaacggct
    22501 attacgcgac gcgtcgagat tttttttcct gaattttcat aatatgtaat aatatctccg
    22561 actaaaatct catttgtatt tttcggtcgt acatatatca gatttccttg tgcataatat
    22621 ggactcattt ctttgtcctc aataatataa ggagtcaaac caaaaattcg gggaaccaag
    22681 acgacgagaa gaataagaat taaaaatata atcaacgtta tagaacttat aaaagttatt
    22741 tttttgtaat caggtatcgt tttcaacttc tttctttatt caatccttct taatcatata
    22801 ataacgataa attttttcaa attatagctg taaaaatgtt tccctttaat aattatatgt
    22861 ttctaattct atttcctcat ctttatctca ttttttatta taaataattg acgttagttt
    22921 aaaaaacgtt taccctaaca ttacaagttt tagaaaggaa tacctttatg tcttttttta
    22981 ataacatat
//
LOCUS       DANKAY010000040        22968 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000040 DANKAY010000000
VERSION     DANKAY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..856)
                     /locus_tag="PWY98_001506"
     CDS             complement(<1..856)
                     /locus_tag="PWY98_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2037262.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRY"
     gene            complement(1424..2491)
                     /locus_tag="PWY98_001507"
     CDS             complement(1424..2491)
                     /locus_tag="PWY98_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2037263.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2511..2990)
                     /locus_tag="PWY98_001508"
     CDS             complement(2511..2990)
                     /locus_tag="PWY98_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HDL2037264.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(3007..3468)
                     /locus_tag="PWY98_001509"
     CDS             complement(3007..3468)
                     /locus_tag="PWY98_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2037265.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3482..4732)
                     /locus_tag="PWY98_001510"
     CDS             complement(3482..4732)
                     /locus_tag="PWY98_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL2037266.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(5110..6561)
                     /locus_tag="PWY98_001511"
     CDS             complement(5110..6561)
                     /locus_tag="PWY98_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2037267.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6882..7646)
                     /locus_tag="PWY98_001512"
     CDS             complement(6882..7646)
                     /locus_tag="PWY98_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2037268.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7674..8372)
                     /locus_tag="PWY98_001513"
     CDS             complement(7674..8372)
                     /locus_tag="PWY98_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2037269.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8384..9163)
                     /locus_tag="PWY98_001514"
     CDS             complement(8384..9163)
                     /locus_tag="PWY98_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2037270.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(9179..10123)
                     /locus_tag="PWY98_001515"
     CDS             complement(9179..10123)
                     /locus_tag="PWY98_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2037271.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(10169..10948)
                     /locus_tag="PWY98_001516"
     CDS             complement(10169..10948)
                     /locus_tag="PWY98_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037272.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(11328..13403)
                     /locus_tag="PWY98_001517"
     CDS             complement(11328..13403)
                     /locus_tag="PWY98_001517"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2037273.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(13405..14322)
                     /gene="glyQ"
                     /locus_tag="PWY98_001518"
     CDS             complement(13405..14322)
                     /gene="glyQ"
                     /locus_tag="PWY98_001518"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2037274.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(14708..15526)
                     /gene="recO"
                     /locus_tag="PWY98_001519"
     CDS             complement(14708..15526)
                     /gene="recO"
                     /locus_tag="PWY98_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2037275.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(15669..16568)
                     /gene="era"
                     /locus_tag="PWY98_001520"
     CDS             complement(15669..16568)
                     /gene="era"
                     /locus_tag="PWY98_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2037276.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(16583..16978)
                     /locus_tag="PWY98_001521"
     CDS             complement(16583..16978)
                     /locus_tag="PWY98_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2037277.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(16962..17438)
                     /gene="ybeY"
                     /locus_tag="PWY98_001522"
     CDS             complement(16962..17438)
                     /gene="ybeY"
                     /locus_tag="PWY98_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2037278.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(17467..19659)
                     /locus_tag="PWY98_001523"
     CDS             complement(17467..19659)
                     /locus_tag="PWY98_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2037279.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(19683..20654)
                     /locus_tag="PWY98_001524"
     CDS             complement(19683..20654)
                     /locus_tag="PWY98_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2037280.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            21359..22738
                     /gene="brnQ"
                     /locus_tag="PWY98_001525"
     CDS             21359..22738
                     /gene="brnQ"
                     /locus_tag="PWY98_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2037281.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         6491 a         4813 c         3787 g         7877 t
ORIGIN      
        1 catatctaac tgccttttga atacaagtac gacagcgtcc caacaaaagt ctccttgttt
       61 tgtttacaaa tatctcattt tctaatatat agcttaaatc gccttcaatt tttttaacta
      121 attcaatatt acaatcgaac tcattatctc taaaattatg tgtcaatgaa ttattattat
      181 tacatctata ataatagata caatctttaa taaaacttgt tatttttgaa tgttgcaaat
      241 atgaaatatg gaatatgata tcttcagata tataatccct ttctgacgga aacttgatat
      301 tgtgctgttg aattatctca gatgaataca gcgcatgcca aactgacatt ggcaatataa
      361 aatcgacttt ataactcggc tcagtgccaa tcattccaag caacactttt tcaattatag
      421 cgttatttgt aaatatcgta ctatcatatc ttgctggctt tgaaataata ttattatcat
      481 cataatattc ataatatcca gcataacatg tgtctgcatt actccttttt ataccattta
      541 acattgcttc aatataatta gctgcaacaa aatcatcaga atcaataaaa gcaacatatt
      601 ttccttttac atgttcaagt cctgtatttc ttgccaatcc caagccagca ttatttttat
      661 gaataatacg aatccttgaa tcctttaatg catagtcttc acatatttta ttagatgaat
      721 ccttagatcc atcatccaca attataatat ctaggttctt atatgtttga ttacacactg
      781 attccaaaca acttcctaca tacttttcaa cattatatac aggtatgatt acactaatta
      841 gatcactcga attcatcctt ctatcctttc actcacgttt tctcaattag tcaatgacgg
      901 catcaacgat tttcttaaat tctctgaaaa aaatagactc tgatcttgat gatgtgaatg
      961 acatactatc ttcttctaga aaaattattt cctttaaatt atttattaaa tcaataatat
     1021 gatcctcgta agtatgcttt cccttggaat aatgaaaata tacttttgta ttacacaata
     1081 ttgcaatttt atttcttaat atgattattt cactcattca ttactgccac atatacacaa
     1141 cttataacca ccccctgcaa agtttagggt aagttatcag ataatatctt cctcttcatc
     1201 atccagaata caaaatctat ttatttatag aaatatcatt aatgttttta tataatgtac
     1261 cgtgcttgta ctcttgctct attacaagca ctaaaacact actattcatt tgaattctta
     1321 cacggataaa aatttacatc gtctatttca atattttttc tctctatttt attcacttaa
     1381 taagttcatt acataatcga aacatttttt attttccttg tcatcactcc ttgctccctt
     1441 tttgatacag atttaaaatt ctatcaaaga aatttccatc gttaaatttc gttattgcca
     1501 aatccttcga acaacgtgaa aattttacgt atttatcttt atcattgatt tttagtattg
     1561 cagatgtcaa cgctgatacg tcaccaggtt gaaacaaata tccattttct ccatcacgta
     1621 ctgctgaaga aatatctcct acatctgtag caattacagg cattccataa gaaatcgcct
     1681 ctagaataga tataggcaat ccctcgttat aggacggtaa aatcatcaca tttgatcttc
     1741 tatagcactc ctcctttttt tctcccacaa cccaacctgt aaattcaaaa tacgattgca
     1801 aattatattc ttctatcaat tctttgagcg atatttcttc atcacctgct ccagcaatct
     1861 caaaaataaa ctttttttct ggatattgtt ctgcaatgtt tttaactgct tctaccaaat
     1921 cgtgtacacc ttttcgcttt atcaaaactc ccataaacag aaatcggcac atttttgaat
     1981 tccactttat ggtatattct ggaactacaa tagaattttc tacaacaaca gttttacaat
     2041 ttggctcaat ttctttaatt atcttcttcc atttttctcc aagtacaatc gtggaatcag
     2101 actctcttaa cagcaagcga atctgatttt gtttttttac agaaacacta tcataccatt
     2161 ttttaaattc cgatccatga agatggatga ttacaggaat atttaataat ctaaaccact
     2221 tattcagcac atatttccta ctaaaactcc ctctgtacga catgtgcata tgaataatat
     2281 taggtttact caataaagag atacctataa ttttagcaaa tgcatacata aaatataaag
     2341 atttaaatat tgcatttcca cccttataac tcggtataaa agtcatctcg atattctttt
     2401 tttcccaatt aaaattgagc atttgtgtaa ttatacttgt aattccacct tttaccgagc
     2461 tgtcattacc aatcataagt actctttgca taacgcttta ctccttatat ttatattttt
     2521 tctattaata cctctatcga attaataatc gtttccgtgt tagatacata cttgttaaac
     2581 atccgaactc ttgtttcttt aacggcatct tctaaatttt ctaattcata acaaggatag
     2641 ataatattca tctcatcaaa ttgtctaatt aactgcagtt ggtgatcatc gacatgctca
     2701 ccatacttag ccaatcgagg aacagcaatg acccttttac ctttttttac ggccccaatt
     2761 atagcacctg tacctccatg agtaatcacc atttctgctt tttgcatagt ttcagtaaac
     2821 tcatccctat ttagaaatgc tttataggaa taatttagtg gtttataatc actataacca
     2881 ctctgtgcaa atgtactttc gattattact cctttttcaa ttaaatcatc aatagctttt
     2941 aacaatctat caaattgaaa tttttgtgaa cctaatgtta taaatatcat ttttttctcc
     3001 tattatttaa tatatcccac ccaaataaat agcattagga taaaattgta gcatagtttc
     3061 ccactgaaca taaaattgat ctgcaaaatg atacatcaat tttcctgtct gagtagggct
     3121 ggttactttt gcaaacgatt caatataaat cagttttttt cccatcattt ttgttattaa
     3181 gcagataggg atcatagcta aaacacctgt acaaatcacg agatccggtt tttcctttat
     3241 atatataata attgatttga ataaattaat aagtatagta cacaaaaaaa acttttcttt
     3301 tctatttact tgtctcatgt agtacgtttt ttcgcctttt gactcagctg aatattctgt
     3361 ctgttctgtt ataacaaaac tctcatactt ctccatcagt ggttttaaca tcattaactg
     3421 ttcataatgc cccccagagg aagccgcaaa acatatcttt ttgttcatat taacctctct
     3481 atcagtcacg ctgaaaaata tctctagtgt aaactttttc tttaatatca tccaagcagg
     3541 tatcatatcg attggcaata atagaatcac acatcgcctt aaactgctct aaatcgttga
     3601 taaccttact tccgaaaaaa gtctctccat ctttcagtgc aggctcataa atcaccacag
     3661 tcgcaccttt agcttttacg cgcttcataa ctccctggat tgaactctga cgaaaattat
     3721 cactgttgct cttcatggtc agccggtata taccgattaa aactggattt tccctgttga
     3781 catcatactg attatattca gcgtagttgt aatagcccgc catctgaaga acacgatctg
     3841 cgatgaaatc cttacgggta cgattagatt caacgatagc ctcaatcaaa tttccaggaa
     3901 catctgcata gtttgccaac aactgcttgg tatcttttgg caagcaataa cctccatatc
     3961 caaaggatgg attgttgtag tgagttccaa tacgaggatc gagacaaaca ccattgatta
     4021 tctgctgagt attcagcccc ttcatttcag cataagtgtc taattcattg aaatagctga
     4081 ctcgcagtgc taaataggta tttgcaaaca gctttactgc ttcggcttca gtgaatccca
     4141 taaaaagagt atccacgttc tccttaattg cacttttctg cagaagtgtt gcaaaaacat
     4201 gagcagcatt taacaagcgc tcatcagtca tatcagtacc aacaataata cgagatggat
     4261 acagattatc atataaagcc ttgctctcgc gcagaaactc tggactaaaa ataatgttct
     4321 tactccccgt tgtctcacgg actgattcag tgtagccaac aggaattgta gacttgatga
     4381 ccatgatggt atcaggattc acgtccatca caacctcaat aactgactca acagcagaag
     4441 tatcgaaatg ctgagtacga ctgtcatagt tagtcggtgt agcaataaca acgaaatctg
     4501 cggatttata agcgctcctt gcatccaaag tagcagttaa gtcaagttct ttttctgtaa
     4561 gatacttttc aatatattca tcctgaattg gagacttccg tttattaatc agttcaatct
     4621 tttcagggat aatgtctacc gcggttacct ggtgatgctg cgccaatagt gtggcgatac
     4681 tcaaaccaac ataaccggtg ccggctactg cgatgttgta ctttttcttc acaatatctc
     4741 ctctttcctc tcttaagtat ggagaatgtt actcctacgg ctattcatca tgtcaaaata
     4801 acacagctta gaatgttgat aaaataattt tccagaatgt gataaaatct ctttcattta
     4861 taaaccacac atctaatttt atctattctc cacaagacat gtagagtatt ctttaataac
     4921 aagatacgac cttactaaat acttcaaaaa attatcatat ttaattcata aatataaatc
     4981 ttcattttaa atgtatgccc aaaaatttat ctgtaatgcg tcatcaaaat ctagattcca
     5041 aatctttatc aatttttatc cactcattct accactttag taatattttc tatttataac
     5101 actttcgtcc tacattgatc cccccctctg aagtacagtt tttactgtct taaataaaat
     5161 tttaaaatct aaccccatac tccaattttt tatatattcc gtgtcaagtt ttgctacttc
     5221 ttcaaaatca gtaatatcac ttctaccact tacctgccac attccagtaa ttcctggctt
     5281 aatagcaatt cttgcacgat gatgataatc atattcttca tactctgatt taaggcatgg
     5341 tcgaaatcct ataaggctca tctttcctgt tagaacgttg aaaaattgag ggaattcatc
     5401 taaagacgtg gatctaataa attgaccgat tcctgtcttt tttgttccat ctggaagtat
     5461 tttatttccg ataattcttg gatcaaactc catcttaaac ataagattat tgctcatctt
     5521 attttctttc attaattcta cttttctttc ttctgcatcc atatacatac ttctaaactt
     5581 atacatcttg aacggtttac cattctgacc tatacgttct tgtgcaaaga aaattgggcc
     5641 tggtgaatta atataaataa gtggagcaat aaagataaaa ataattcccg ttgcaataca
     5701 tccaacaata cctgcaagaa tatcaattgt tcgttttata aatgcctgtt gagttgtcat
     5761 aacattgata cttgttgtaa gtactgtata ttctcctata cgaccaatag attgttttcc
     5821 caaagcacta tctgatactt ttgcaagatt caaatgtaca gtaaggccca tttcagagca
     5881 tcgttcaata aattctttca tacacgaagt acttttattc aaaataataa agacttcatc
     5941 tacccatccc tgacataagt aatccgctgc atttttggaa tcagaaacaa ctgtcactcc
     6001 ctcaacatct tcaccaatca tgcttttatc tatgataatg acaccattta ttttatacat
     6061 tttatagtta tgctctttca cattacgaat aactacttca gcaatactac tagatgtaac
     6121 tataataagt gatttgttcc cctcattttt cattttatgt ttcaagtaat gtttccataa
     6181 gaggcgagtt atatatgtaa ttacaacgta taaacctccc atcaagaaaa gaacgactct
     6241 agaataatca tttccaccct gaacactaat gagatacaaa cttcctaaca gcaccaacat
     6301 aatcgcttgc tttacagaag aagcaaattc tatatagtac cctcttttta atacacctct
     6361 atatgcttca aacatgaaaa taactaaaag atctgccagt tcaatgaaga ctgccatgtt
     6421 ttgatacatt ggaatcacat aaggattcca atctacctgg cgtaagacat acgatagata
     6481 aaaagcaagc tggatacaca ataaatctaa aataataaaa tcaatatgtt taacccatcc
     6541 ccttgaactt ttcttgtaca ttccttttta ctcccctctt tttaataaaa taaattgtat
     6601 tacttgtttt ctctcctaaa tatctttctg aaggattatt gactcgacaa attggcttgt
     6661 ggatattctt atatatagaa catccgtaag ccaacttaaa actccttaac aaaatttttt
     6721 tactcatatt gtagatcaaa acaagtatcc aagaaatcat attttatttc ttttttctgc
     6781 ttactcaaca acagctaaaa gaaaaatcat cataaacaaa ttcaactaag ccaacgatcc
     6841 ccttgtgaaa tcaagttttt tcttgattta cgaataaaga gctaaaataa accaaacttc
     6901 ttctttttta cctcttcata gactggataa ctgatttcat ccccgttgat cagatccttt
     6961 gcgacttgtt tcatctgtac tactttttcc ttaccaaagt cctttgcaat ctgctcatag
     7021 cactctttta agtaaaaggt gcgcttagag accccatggg catcagaagc agccatctgc
     7081 acgagattat gtttgaccat cgttttggct gttttctgga tgtctttgcc aaatacccca
     7141 acataacttg gcgcagtgag ttgggccaaa catcccatat ctaggaacgg aagcaaatga
     7201 tttgggtctt cgcgaaaatg cacgttgcgt tctggatgga cgattactgg tgtctttcct
     7261 agttctaaca actcaaaaaa aagccgttct gcatataacg gcacttccag tgtcggaaat
     7321 tcgatcagaa gatatgtatc atctaaatct gtaaataata tttcatctcg gtggatgtct
     7381 tcgatgagcg ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaactgt
     7441 cttttctcta attcggcttg taacgaagcg actagagaaa tgacttgtga tttttcattg
     7501 ctgtattttc cattattgtg gtgtggtgtg cataaaatat gcgtgatgcc ttgctgaatc
     7561 gctttttcag ccattgcaat acttgcttct aagttttctg ccccgtcatc gattcctggt
     7621 aggatatggc aatgtagatc gatcatttct cttgtctcct ctcttcttat tccttaattt
     7681 ccgtagtaat aaccagcatc tttgctatgt tctgctccgt tgtacacaac acctaagact
     7741 cgtgcttgta ccatctccag cagttctttt gctttagtca aggattcttt acgtgacata
     7801 ttttcacgta cgaccaataa agtgccatcc gtttttaaag acatgatctg tgcatctgtt
     7861 acggctacta ctggtggcat atcgaatatc accacgtcaa ataactgtct tgcttcggat
     7921 aggatctgat ccattcttgg cgagcttaac aattctgatg ggttgggtgg cttaggtcca
     7981 ctaggcaaaa tcgacaagtt ctcgactggc gttcgttgga tcacatctgc tacacttcct
     8041 gatgaactaa gtgctgtact taatccactg gcattgttca gctggaatgt cttatagacg
     8101 acgggtttac gtaaatctgc atcgactagt agaactcgtt gacctgattt agcaaaaacg
     8161 actgcaatgt ttgctgcagt ggtggatttc ccttctccag gacctgatga agtcacgaca
     8221 atcgttttga tttgctgacc agctgcggag gcaaactgga tattcgtccg gatcgtacga
     8281 tattgttcgg cgattggtga agatgggttt gttaatgtga tcaaactgac tgcatgagtt
     8341 tgtgctgctt tttgtttctg tgttcgtgcc atttttcttc ctcctagact cttgagcggc
     8401 ttctgcgtga tggggctgtt ttatcgtttg tagcttttgg tcgtgcagga gctgttcctt
     8461 ttttacggat accagcgttc aattctttcg ctgacatttc tggaactgtt cctaagattg
     8521 ggaatcctaa tgtatctgtc acatattttt catctttcac tgtccgatcg agtaattcca
     8581 ataagaatgc cagacctaca cctaccatca ttcctaacat taagccagtc attaaattca
     8641 atttattatt tggcgaaaca ggtgtcgtat tggcaactgc acctgaaatg atcgagattt
     8701 tatcaacatt catcacatct tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga
     8761 tctgttgagc agccactgca ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt
     8821 ctgcctgatt aactgagatg gcatctttga tttgttcagc agtcattttc acattgtctt
     8881 ctgtttctaa acgatctttg acttcattga gcactaaatc actgacgatc atatctttat
     8941 acgtattgat catttgcagg ttcgtattga catcattgac atttgcagtg tctgattgtg
     9001 gcagtgtcac gatcaattgt gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta
     9061 cgattcctga gatggccaag ccagcaaaca ttgagatgat gattattacc aagtgtttgc
     9121 gtaatgtttc aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct
     9181 atttattctc tagattcatt tgatctttta attctttttt cacacgatca agttcttgat
     9241 cactgacttt ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt
     9301 gcttaatcgt accaaatgcc tcccgataat ctaaagcaat ttttttcatt tcatcaaagt
     9361 tcatgtctgt tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac
     9421 cgtttagact caatactttt ttcgctacgc cttctacgat ctttctttgg cgcccttgtc
     9481 taccgtaatc accatttggg tcttcataac gcatacgaga ataagataat gcctgatccc
     9541 cgttcaatgt aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat
     9601 tattgacttc gataccacca actgcatcaa cgagttcctt tagtcctttc atattgatag
     9661 agacatagtg atcgattggg atgtctacat atttttgtac ggtatccatc gccatggcag
     9721 ctccgccgaa tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga
     9781 catacgtatc tcgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg
     9841 caaccatcat cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatcc
     9901 ccatcaatag aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc
     9961 cttgggttcg ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata
    10021 attttgctcc tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca
    10081 aaaatatttt tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct
    10141 aaccttgctt gaatcaacgt tttaactgtc aatagcttat attgctatag cccatttgct
    10201 gagcaacagt ttgtgctttt gttgcgtatg cggctcttat tttttcattc cggatctgct
    10261 ctatttgact gagcgccgtc aaatattgat cgtaagtagc ggtagctgtt acttgaccgt
    10321 ctttcagcat cgtatcgaat agctgatcta gttgcgtgac ttgtgcaatt tgggtatcag
    10381 cgaatcctag atattgcata acgactgttt tccaagcact atccttaaaa aagctcatat
    10441 tttcacgtac atgggcgaca tctgcacttg ccaacttttc tttgatgatg acatcatcca
    10501 cagcttgctg ccagtttgat acgtttttag taaataattt attgacagcc gtctggatct
    10561 tcagttttga ttctgcatcc tctaactgcg ttaagactgc tttcttttct tcttggatcg
    10621 cagcagcttc tttaggtaat tctgattcct tgatgtcata atcatctgcc gaaagtttga
    10681 tagaagatag tttatgtgac aaatttgaaa ctgtttcttc tgttatttca ggatttagga
    10741 aaattttttc ttttgtactg tacagcttat ctacttcttt tgataaagag gtcacggtgt
    10801 ctttttgatt ggtgatggca gattctgcgt aattgatccg ttgatgatat tgccgctgat
    10861 tattgagatg gtatccaaac aagcaaatca caatagcgcc tgtgcctacc gccacagtag
    10921 ccaataactt gacgaacggc ttttccatag attcactcct aaatattgat gatagataag
    10981 taaagatgtg attcattcac atcagaatga gcgcttttta ttttaaaagc ataattaaac
    11041 taatatattc atagaaatat ttataaacag gttttcccta tataatcata ccacatacag
    11101 tttttttgac tagggaaatt cctaatttaa aagtaaataa aacagtaaca agattataat
    11161 taatcgtttt catttttatt tcctcacttt tcatgagcgt tttgaaaatt cgaacaaaga
    11221 ataaaataac aaaaattatt aaaatagaat taataacagc tatttataac aaaaaaaaca
    11281 gagacacagc acgtttcgtg catgctctct gtttttcaaa caaaagacta ttttgtgatg
    11341 atttgatcca agcttgctaa tgacaaagcc atggcagcta tttgacttag ttgtgttagt
    11401 cggttcttac gtactgctgg ttcatcagcc atgaccatcg tttgatcaaa ataattttcg
    11461 atcagtggac gaagagcgac aagctggtcg tagttttcgt ggatcgtttg gtcagcaaaa
    11521 cttgctttca ttgcttctac tgctttgtgt aattctttct ctgcgtcgtt ttcgaacagc
    11581 tcagggtcta cttctgattg tttttcaagc tgcgcttttt tcgctaagtt cacgacacgt
    11641 gtcaaagctt ccattgaagg tttgaagtct tcatctgcaa gatgttgttt gaacaattgt
    11701 gctgctgcaa atacttttgt cagatctttt tgttccgcat tcaaaacagc atcgatgaca
    11761 tcatgacgaa tattcttcgt tgtcaataat tggcgcagac ggcctttgat aaagtcgatc
    11821 acttgttgtt gcccttctga taaaccaatg ccgaaacgat ccgtatcttt actgataaca
    11881 tcgctaatcg tttcttgcag aacagacaaa ggaaaggccc attttttcga ttcgatgatc
    11941 cgaacgacac cgtatgcttg gcgacgtaag gcataaggat cgtttgaccc gcttggaatc
    12001 atcccaactg caaagaaaga aaagatgcta tctaatttat ctgcaatcgc taaaacagaa
    12061 cctactgtag actgaggtag ctctccttca ctggatatag gcatgtaatg ctcacgaatc
    12121 gctgtcgcta cagcaggttt ctcgcctttc agaagagcat atttttcgcc catgatccct
    12181 tgtagttcag gaaattcccc taccatgttg gttactagat caaatttata gatttcagca
    12241 gctcgttgca aatccatcaa ttcttcctca gatagtccta cttgttcgcc aatgatacgg
    12301 gcaatgactt gtacccgttg cattttctca tagatactac cgattttttc atggaaagta
    12361 acagttttta gtttttcaac gtattggtcg attgaagctt tcttgtcttc attataaaag
    12421 aattccgcat cttctaaacg agccgtcaat actttttgat tgcctcgaac cacatcttcc
    12481 aattttaccc gatcaccatt acgagcagaa ataaaatgag gaagcagcat accttgatca
    12541 ttccgtacct cgaaataacg ttgatgttct ttcatagaag tgactagtac ttcttcagga
    12601 acagaaagat atttttcttc gaagtcgcca gaaaagacag ttggatattc gactaaattg
    12661 ttgacttctt ctagtaaatc ttcgtctaga tcaataaccc agtgattttt ttctgctaat
    12721 tcgtttgctt gtgtaacgat catttcttgt cttttttgcg gagacgcaat gacgaattgt
    12781 tcattcaatt tttcttcata ttcttttgca tgcgcaaatg tcacatcttc gcctaagaaa
    12841 cgatgacctc gagaggctct gcctgttttg acatccaaga tttcgaatgg gattacttcg
    12901 tcatctaaca atgccacgat ccagtgaata gggcggatat attcaaaatc gtattctgcc
    12961 caatgcatcg ttacagggaa agtcaaactt ttgatcaccc ctgctaagcc tgtcaatact
    13021 tcttttgctt ccttaccatg agtgtatttc gttacataga catattccac accttttaat
    13081 tctctaaaag tgatagcgtc tgtcgtcaga ccttgtccgc ggacaaatcc ttccgctgct
    13141 ttcgtccagt ttccatctgc gtccaaagca atttttttgg ctggtccttt gacttcttct
    13201 tcactgtccg cttgtcgttc tggaatattt gttactttga ttgctaaacg gcgcggtgtg
    13261 gaaaatgttt cgatcgtatc ataagtcaat tggttttcat ctaaaaattt tgctgttttt
    13321 tcttctaatt gtttcatgct tggtgtgaca acatgggcag gcatttcttc cagcccgatt
    13381 tcaagtaata agtcttttgc catcttattc agcctccttc acggtttctg gatttgctgt
    13441 gtttttattc aacaaaggga atcctaattt ttcgcgttct gccacaaaga ttttggctac
    13501 agcacgagcc atgttgcgga tgcgagaaag ataaccggca cgctctgtta cggatactgc
    13561 accgcgggcg tctaataagt tgaatgtatg gctacatttc aagatataat catacgccgg
    13621 atgaacaaga ttttcatcga tacagcgttt agcttctttt tcaaaacgat caaagttgct
    13681 tagtagcaag tcttggtcgc ttacttcaaa agaatatttg gaatgttcat attctggttg
    13741 tttgaagatc tcgccatatt tcacgccatc agcccattct aaatcataga cactttcaac
    13801 ttcttggata taagaagcaa gacgttctaa tccataagtg atctcagaag ttactggttt
    13861 acaaggcagt ccaccaactt gctggaagta cgtgaattgt gtgatttcca ttccgtcaag
    13921 ccatacttcc caacccacac cggcacagcc catggatggg ttctcccagt tgtcttcaac
    13981 aaaacggatg tcgtgttcta atggatcgat tcctaaaagg cgcaagcttt ctaaataaag
    14041 ctcttgaata ttctcaggtg aaggtttcat caccacttgg aattgatgat gctgatacaa
    14101 acggtttggg ttttcaccat aacgtccatc agctggtcgg cgagatggct ctacgtaagc
    14161 agcattccat ggttctggtc cgattgcccg taaaaaggta tatgggctca tcgtacctgc
    14221 tcctttttct gtgtcataag cttgcatcag catgcagccg tttgatgacc aaaatttttg
    14281 taatgttaaa atcatatctt ggacggtcaa ttttttactc atctgttttg cccctttcaa
    14341 ttttaaagcg ttgctgtcaa ataagaatac aggaaagaaa acgacaaaaa atccctatgt
    14401 ctgattacag acatagggac gaataattcg cggttccacc ctacttccga ataaagattc
    14461 ggcagcttca tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg tccggctccc
    14521 actctcccga actcgctaaa ccacgtgttt tttactgatc ttggtcaatg tacgtattca
    14581 ttttgcagtt ttcttgtttc tataatagat cctatcactg aacgatctat ttaatctatg
    14641 gttaataata gacggaactc cctgtgtttg tcaaatggtt ttgatttttt gttgtatgtt
    14701 ttatttacta ttcttctttt tcaggtgtct ctttttcttt ttcagacatg tctttctctg
    14761 acgtctttaa tacgtgtgcc caagacttca tttgattgat gaactttttg cttttcaaat
    14821 ggataccgac atattcatca tacagctggt cgatgagttc acggattgct tgtttggttt
    14881 cttctttcag atggattcca ctaattttat cataagaaat agctgaaaac atccgaacga
    14941 aataaacagc tcgaggatct gcatgataac ggtgatcgtc catcgaccaa tgtctttcgc
    15001 atatcacacc accatatttg gaagaatagt caaattttcc ttttgtttcc ccgcatacga
    15061 cgcagtgtgt ccagattggt gcaattccga agcgttgcag tatttggact tcaaaaatat
    15121 tcgtgatgat ttctgcatcg tctccttggt ccatccgttg cagtgcttgt tgcgtaaact
    15181 ggaacaaatg cgggtcgtac tgatgatctt cgattgccgc atctactaga ttcaataaat
    15241 atgtgccata gccgctcaaa aagatgtctt cttggatgtg tcgaaacgca tggacatctt
    15301 tgacactgct taaaaatgac aaaccttcac ttcgaaaatt cccgatataa actgcttcag
    15361 taaaagggag caaagccgga gctaagggct gattttgccg atggatctct ttgatataaa
    15421 acatcatttt gccagaagat tctgtaaaga ttttgactaa tttatctttt tctttgtaat
    15481 tacggctaga taaaatcaag ccttttgatt ctgcaagctg gctcatattc tccctcctaa
    15541 aaaataaatc tggaaaagca gattctgccg atccagatca tttatgttgc gatcttccgc
    15601 agcttttctc aacacgcgga tttttattca gtttatttct atacctaatt tacaagaagc
    15661 tcagaatatc agtattcgtc ttttcggtag ccgaagtctt gcagatagac ttttttgtct
    15721 cgccagtctt tttgaacttt gacccaaagt tctagaaaga ctttgtctcc taaaagattt
    15781 tcgatatctt ttcttgcttt cgttcctact tgcttcagca tttttccgcc tttgccgata
    15841 atgatccctt tttggctatc tctttcgaca ataatcgtag cctggatatg gattttatca
    15901 ttctcgtttc gcttcatcga atcaacaacg actgcgacag aatgaggcac ttcgtctcgt
    15961 gttaaaaaca agactttctc acggatcaat tcagaaacga tgaagtattc aggatgatca
    16021 gtcacttgat catctggaaa atattgtggg ccctctggca tttcactgac taatacatcc
    16081 atcaaacgtt cgacgttgtt tccttctgta gcagagattg gaatcacttg tgcaaaaggc
    16141 atctgcgttg aataatcttc gatgattccc aacaactcgt ctggatggat tttgtcgatt
    16201 ttattgataa tcagataaac aggagaatgc acgtttttta gacgttcaat aataaaatcg
    16261 tctccccgac cgcgtttttg atcagcacta atcataaata agaccgcgtc cacttctttc
    16321 aacgcactgt aagctgcttc taccataaaa tctcctaaac gatgctttgg tttatggatt
    16381 cctggtgtat caataaaaat caactgagct tctggtgtag tgtatactcc ttggatttta
    16441 tttctagttg tctgtgcctt gtcactcatg atggcaattt tttgacccac gattcgattc
    16501 aataatgtgg atttccctac atttgggcgg ccgacgattg cgacaaaccc tgatttatgt
    16561 tctggcatat atgttctcct ttttaattaa acaacaaatg atatatttta gggacaaata
    16621 aaatcaaccc aacgatgacg gtaaaacaag cggtcactaa aactgctcca gctgccatat
    16681 ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc acgacatttt
    16741 caaaaacagt attgatgatc tctactatcc atacgagaaa aacagctaaa agcagccata
    16801 accattctat ccgatcaagt tggaaaatca cgcccataaa caaagcgagg attccgaacg
    16861 caacatgttt cctcatgttg cgttcttcct caaaaacagt tttgactcct tggacggcaa
    16921 attccacgga catcaggaaa tgtttgtttt tctctacgtt tttatctttt aagtccatag
    16981 gcatctaaga tctctttctg taaaccaaac atttctttct catcctctgg tgtcatatgg
    17041 tcatacccgt tgatatgcaa gaatccatga accgctaaaa agccaagttc ccgatcatac
    17101 gaatggccgt aagcttctgc ttgctctgca gcacgttcag tagagatcat gatgtctccc
    17161 agtacacgag gcaatggatt ctctccatct tcaaaaatca ctgggatttc gtcttcgccc
    17221 tcttcttcca aggcaaaact gatgacgtct gtcggtgcat cttttccacg gtaatcacga
    17281 ttgattactt ggattgctgc attgtccatg aaagtaacag acatttctgt ttcttctggc
    17341 aactctaaat atgatgctgc aaattgcagc aagtcgtcga tttctttcat tttctcttct
    17401 gagacttggt ttgtttcatc catgaaggta atatccatta tacatcgctt ctttctattc
    17461 ttcttgctat tcttcttgtt cttcttttat cttctcaact tccgatttca ttttaggata
    17521 tttgatccga gaatggaagg tactgcttaa accgctgatc aatgattttt ctactttacg
    17581 gatttctttt aacgtcaatc cactgtcgtc caattgtcca tcggaaatac gctcttcaat
    17641 aagattatgg acaaaacttt cgattttttc gctcgttgga tggtccattg cccgtactgc
    17701 cgcttcacaa ctgtcagcta tattgacgat tcctgcttca cggctttgag gttttggtcc
    17761 aggataacgg aattgctctt ccgtaacttc cgggttgcgt tcctttgcct taacgtagaa
    17821 ataacgcatc agcgtggttc catgatgctg tctacaaata tcgatcacca tttgcggcat
    17881 attataatct tccaatattt tggcaccatc tgtcacatgt ccaaagataa tatgtttact
    17941 gtcttctggc aataagaaat tgtgcggatt ttccgctcct gaaggcaagt tctcaacgaa
    18001 aaagctggcg tgtttgatct tgccgatatc atgataatag caagctactc gagttagtaa
    18061 agaacgtcca ccgatttctg ctacagcatt agcacttaga ttggctacca tcatgctatg
    18121 gtgatacgta cctggtgctt cttccagcaa ctgtttcaat aaaggatgat tgggattgct
    18181 gagttcattt aatacgatca cactatcatc attgaccatc aattcaatat aggggtgcag
    18241 acctatcgtc agcaagaatg aaaacagtgc acctgtgaat ccgcagacta gcgttaacca
    18301 agtcgttgca tccccaaagc tcattccttg gtagataatc aaaatgacat ccatcatcac
    18361 aggaaaaata atgacccaca tagcagcgga taacccttgt tctgagatcc gtttgcgtct
    18421 gacgatcgtt gccatgagac cagaaaatac ataggacatc aaaataactg tcagaaagtt
    18481 cgtgccaatc gaatcataaa aaatgaaaag tgcggatact gcttgaaaaa taccagccat
    18541 gatccctgct cttcgattca agaaaaagct taaaacaagt ggagcaaaag ccgcggggta
    18601 aaacagcgga atataagcag cttgttctgt ttggaagagt tggaaaaatt tcattagtac
    18661 gacgcttact gacattgccg tcacgtagaa aagtacgtat tttgttcttt ctcctgcatt
    18721 ttggaattga agtgaattat aaatcagtac accgatttga agcaaaacag ctaaaaccat
    18781 ggcaatgagt gggaaaatag aagtcgtttg attcgtcaat cctagtaatt ccaatttttt
    18841 catcgctgaa gcatcaattt gatttccttc gcgaacaata atttctcctt ggaagatcat
    18901 cactggttgt acagcttctc gtgcagattg ttttaattct tctgtctttt tctcattcaa
    18961 tacatcatta actacgatgc cttgatccac taagtagcga atcatttgtt gctgctctgt
    19021 tgtgacattc aaatattgaa tctgatcttc tgcttcttgt ttaaaagctt ccaattcgct
    19081 ttcacgaact tgtttactca tctgttcgtc aatgagcttc aaactttcat ccctaactgt
    19141 gtgaatttcc gtttcagtca tttcaaatat cgtcgaataa aaattgttcg gcagtttttg
    19201 gtaaaacgca acgttttctg cattcacgtt ttcaaaattt ttcttcagtg tagcaatccg
    19261 ctcttctgta gtaggtttcg ggatattttc atcttccttc gccttatcta ccttgccatt
    19321 ataagatttg ttgatggtat cattcgtctt atcgatcaat tcgaataatt gcttgatccg
    19381 attgttctgg tcatctgcca gatctttttg ataagtatat tctggagtca ctgcttctgc
    19441 tgccagtttt cttctttgtt cggtttcttc cgtgttctca atcgttttat tcgcacggat
    19501 actctcttct gctacttggc cttctctgaa atcaatggcc ttttgtttga cactactaaa
    19561 tgtaacgccg cagacaatga taaaaaacaa gaacagaagg aaaggcaatg ctgcctttcc
    19621 gagtttttct tgtagtttct gcagtgtttt attcaacatg tgcttatcct cctaagcaaa
    19681 gcttattctt gatgaagatc cgccttttca taagctttga tgatttcggc aactacaggg
    19741 tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga tttttttgat ttgctttaat
    19801 gttcgttctg catgaacaag accacttgtc gtcccacgag gcaagtctat ctgacttgta
    19861 tccccattga caatcatttt tgattgataa ccgagacggg tcaagaacat tttcatctga
    19921 gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg catcatccaa tgtccgcccc
    19981 cgcatgtaag ctaaaggtgc gatttcgatc acgcctcgtt ccatcaatcg gttcgtatga
    20041 tccaatccaa aaatttggta taaagcatca taaacaggcc gcagataagg atccactttt
    20101 tctttcagat ccccgggtag aaagcctagg ttttcccctg cctccacagc gggccgcgtc
    20161 aaaatgattt tttgcacttc gccttttttt agggcagcaa ttgctaatac tacggcaaga
    20221 aacgttttcc ctgttccggc tggccctaca ccaaatacga tgtcatgttt ggctaccgat
    20281 tcaacgtatt tcttttgacc gctgtttttt acccgtatag gttttccatt acggtctttg
    20341 acgatctctt cctcatacat ctcaacaaaa taatctaatg tacctttttg ccccattttc
    20401 aatgccgtca caacatctgg cgtacttacg tggattcctc gtttgatcaa ttcctgtaat
    20461 gtccgaatga ctgaggcagc caaggaaatc tgggattgct caccaatgat ctgaatgatt
    20521 tctccacgtg tatggatggt tgtttccgtt gtttcctcga tcagttttat atgcttgtcg
    20581 tgtgcaccta aaagcatgct gacatcatca gcagctgtca atctaatctc taaagaacta
    20641 gactcttccg tcaaaaatag acatcccctt tttttcgatt cattctttcc tattttacca
    20701 tactcagaag aaactatata gcaaataaga aaaggttgta aaagagtcgc ttaaccatac
    20761 gtaccaagaa acgttaaaaa atagccgttt ccattcgcta aaggtctcgt tttgataaaa
    20821 agaagctgag aatgtaagtt atatgaaagt ggagaacaaa atatttcgtt tcattcaaca
    20881 agtattctct aggtattttt cctgtttcaa aaaaagaaaa caaaatagtt aaaatattct
    20941 tgcatttcaa aaaaaacagg tgtattatta cgtcaacaaa taaacataca gcaaagcgtc
    21001 gaggaaaaca gtaggctatt caacgattta ttcccagtga gtcatgtaag tgagaaatga
    21061 caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta gatcattgtg
    21121 gctgcctcca ttatcaggct agcggataac tttatcaaaa agcgtccgtg aatgaagaag
    21181 cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt cccaagtgga
    21241 aaagggcttt tttttatttt aaggtggtgg ttttcatgga atctgatatt agtggcatta
    21301 ctaattgctg caacgtttat atcgtcaatc aaaatgataa ttattggagg aaaaatttat
    21361 ggaagaaaca aaaacttatt catggaaaca actattgatg gtcagttcac ttatcttcgg
    21421 gatgttcttc gggtcaggta atctaatctt tcctgtccac ttaggtcaaa tggctggcgc
    21481 aaactgggtc agtgctggcg taggatttgc tatctcaggt gctcttttcc cattactagc
    21541 tatcttagct gtagtagtga caaaaagtga tggtctgtat gacttagcta aacctgtcgg
    21601 aaaatggtat gctgccttgt tcttaatcct tgttcatttg acgatcggac catttttcgg
    21661 tacaccacgg acagctgcta ctgcttttga aatggctgct aaaccatttt tgcctgaaaa
    21721 atataattca ttaggtatgt ttttattctc tgctcttttc tttttacttg cttatttcct
    21781 tactgtccat ccaacacggt tggtcaaata cgtcggaaag tatctaaact ccttgtttct
    21841 tgcattatta gcaatcattt ttgtcgtttc tttattccat ccaatgggaa atctaaatca
    21901 atctgctgca gatacttatc aaacaaacgc tttaacaaat ggtgtcctag aaggttataa
    21961 cacggttgat gcagtagctt tgcttgcctt aagtgtcact tttgtccatg cagtccgtga
    22021 ccttggtttc aaagataaaa aagtcacaga attaacggca aaagctggta gcctgagcgt
    22081 tgcgctagaa gtcatgatct attttggact tgttcttctc ggtgcgatga gtttgaataa
    22141 gatgtcttta tcagaaaatg gcggaactgc actttctcag atcactaatt tttatttagg
    22201 taacttcggc gctagcttct taggagtgat ggtcacatta ggtgtcttca ctaccgctat
    22261 gggactcgtt gtttcttttg cgcaagattt ccataaactg ttccctaaag tcagttatat
    22321 gacatggctg cggttaacga ctttcgtctc ttttgtcgtt gcaaatgctg gtttagacaa
    22381 tatcatccaa tggtctttac cagtattgat gcttttgtat ccattgtcgc ttgcgttgat
    22441 cttgctttcc ttaacagcaa aatttttcca aaagacacca tttgtctatc aagtaacgat
    22501 gttgtttgct gctgttcctg ccgtattgga tatgctggca aattcgcctg ctttagtgag
    22561 tcaacaaaga gttgtagcaa gcatgctcga attttatcat catcacgttc cttttgctgc
    22621 attaggactt ggctggatgg tcccaacgtt acttggttac gctggcagcc tactgtttta
    22681 ttacgcctat cgcctatcag ggtataaaca agaagcaaat gaactaccag aagaataacc
    22741 aaatcaaaca caatttaaca taagaaatat taaacaggaa aaaaccagct agatgaaaga
    22801 atcctattct ttcgtctagc tattttttag ttctcttttt tacgcgcaca aaaaaactgc
    22861 ggacaatgat tgtccgcagt ttaaatgggc tctataatat ctagtgttgg tgacgccttc
    22921 tgttgccaat actttttctt ttttctaata aacgcaaaca gacacctt
//
LOCUS       DANKAY010000041        22757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000041 DANKAY010000000
VERSION     DANKAY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            144..512
                     /locus_tag="PWY98_001526"
     CDS             144..512
                     /locus_tag="PWY98_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2037282.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            692..1633
                     /locus_tag="PWY98_001527"
     CDS             692..1633
                     /locus_tag="PWY98_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2037283.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1702..2433
                     /locus_tag="PWY98_001528"
     CDS             1702..2433
                     /locus_tag="PWY98_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2037284.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2577..4757
                     /locus_tag="PWY98_001529"
     CDS             2577..4757
                     /locus_tag="PWY98_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2037285.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4777..5259
                     /locus_tag="PWY98_001530"
     CDS             4777..5259
                     /locus_tag="PWY98_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2037286.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5275..6036
                     /locus_tag="PWY98_001531"
     CDS             5275..6036
                     /locus_tag="PWY98_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2037287.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6167..7501
                     /locus_tag="PWY98_001532"
     CDS             6167..7501
                     /locus_tag="PWY98_001532"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2037288.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7808..8293
                     /locus_tag="PWY98_001533"
     CDS             7808..8293
                     /locus_tag="PWY98_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2037289.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8286..8969
                     /locus_tag="PWY98_001534"
     CDS             8286..8969
                     /locus_tag="PWY98_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2037290.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9095..11740
                     /gene="polA"
                     /locus_tag="PWY98_001535"
     CDS             9095..11740
                     /gene="polA"
                     /locus_tag="PWY98_001535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2037291.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11785..12621
                     /gene="mutM"
                     /locus_tag="PWY98_001536"
     CDS             11785..12621
                     /gene="mutM"
                     /locus_tag="PWY98_001536"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2037292.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12585..13214
                     /locus_tag="PWY98_001537"
     CDS             12585..13214
                     /locus_tag="PWY98_001537"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2037293.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13266..13598
                     /locus_tag="PWY98_001538"
     CDS             13266..13598
                     /locus_tag="PWY98_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2037294.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13910..14401
                     /gene="nrdR"
                     /locus_tag="PWY98_001539"
     CDS             13910..14401
                     /gene="nrdR"
                     /locus_tag="PWY98_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2037295.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14424..15797
                     /locus_tag="PWY98_001540"
     CDS             14424..15797
                     /locus_tag="PWY98_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2037296.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15797..16723
                     /gene="dnaI"
                     /locus_tag="PWY98_001541"
     CDS             15797..16723
                     /gene="dnaI"
                     /locus_tag="PWY98_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2037297.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16852..17292
                     /locus_tag="PWY98_001542"
     CDS             16852..17292
                     /locus_tag="PWY98_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2037298.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17436..18137
                     /locus_tag="PWY98_001543"
     CDS             17436..18137
                     /locus_tag="PWY98_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2037299.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18134..19255
                     /locus_tag="PWY98_001544"
     CDS             18134..19255
                     /locus_tag="PWY98_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2037300.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19270..20502
                     /gene="pepT"
                     /locus_tag="PWY98_001545"
     CDS             19270..20502
                     /gene="pepT"
                     /locus_tag="PWY98_001545"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2037301.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20634..21542
                     /locus_tag="PWY98_001546"
     CDS             20634..21542
                     /locus_tag="PWY98_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2037302.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21577..21849
                     /locus_tag="PWY98_001547"
     CDS             21577..21849
                     /locus_tag="PWY98_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2037303.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21860..>22757
                     /locus_tag="PWY98_001548"
     CDS             21860..>22757
                     /locus_tag="PWY98_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2037304.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTV"
BASE COUNT         7675 a         3972 c         4999 g         6111 t
ORIGIN      
        1 aaaaacaacc aacttattta ttattaaaga aaagcgtatt taagtaggac gattctgttg
       61 agagctttcg tagattttcc cattttccta tggtaaactg acaaagttga gaatgttaga
      121 acagatcgtt taggaggaaa cagatgttta cgaatgaaat aaaaatcatg ctttatgtgg
      181 acgatgtaga aaaaaatgca acgttttgga gagcagttgg ctttgcggaa aaagatcgtc
      241 aagaaatgga tgggacgctt ataattgaag taggagttga ggacagtcaa acctcatttc
      301 tgttatatga tcgagcattt gttgagtccc attcacctgg cacagctact agtgccccat
      361 ctttgatgtt ttacagtaaa gatatctttg gactatataa aaaaatgcag acgcaatcag
      421 tcacattagg tgatatggta cagcttggag aagaatatgt ctttaacttt gctgatcccg
      481 atggcaacta ctttgcggtt acaggtaaat aatagaaata aagactttgt ttttctgtgc
      541 gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac aaaaaacttt
      601 ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc tttatactgt
      661 ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc tttattacaa
      721 ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct tttgcaaatc
      781 tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta ctgtgtctgg
      841 aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc gatttgctga
      901 tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt ttgtaagata
      961 ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc aagtgacagg
     1021 tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga cagtggtgat
     1081 tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg tacgcccaga
     1141 tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag aatttatgga
     1201 aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt ctggttggct
     1261 tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta cttatggtaa
     1321 ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg atgaacggat
     1381 cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt ttgacggaac
     1441 accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc
     1501 cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga aacatccgcg
     1561 caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc ggtttacagc
     1621 tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg cgctatacta
     1681 gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga tgtccatggc
     1741 aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca tcagcttgtt
     1801 ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt attaggaaag
     1861 caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga atattttttg
     1921 caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg tggaaaagaa
     1981 acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac tgaaatctcg
     2041 atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg ccccttgtat
     2101 tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa gaaagactgg
     2161 cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga agggaaaaac
     2221 aatacaggta agacaattgt atttggacat acgattacac caatgctcca tggagatatg
     2281 cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg ggctgtattc
     2341 ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga tattgaatat
     2401 cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa ggagctgtga
     2461 ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta gtttaaccca
     2521 ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc attttaatga
     2581 cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca gactatcgtc
     2641 ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt ccttttatcg
     2701 cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt gaaatcgaag
     2761 agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg
     2821 atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc gtgaaaatgc
     2881 aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa gcaacgattg
     2941 ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca gatggagagg
     3001 tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct gccgaagaag
     3061 cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca aaatttcgga
     3121 cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc aaggatgagc
     3181 aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc gtccataaaa
     3241 tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt ctgaaagtat
     3301 tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata gtcaaaaatc
     3361 ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat aaacgcttta
     3421 ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca gatgagcaag
     3481 caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca ctaaaaggta
     3541 aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca gtcgtagatg
     3601 cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca gcgcaagcaa
     3661 aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa gtagatatgg
     3721 tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct gagcagttga
     3781 aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct tctgtttact
     3841 ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa agaagtgctg
     3901 tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc gatcctaaag
     3961 ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct gaacaattgg
     4021 attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca gcaagcccgc
     4081 agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt gtcatttatc
     4141 gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac
     4201 caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa aacattttgg
     4261 acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta acttcagtcc
     4321 aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca cgattatccg
     4381 tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat attttagctg
     4441 ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta ttacgtacag
     4501 atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg gtccgtaatg
     4561 tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt gtccatatat
     4621 cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg
     4681 taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg acgatgcttt
     4741 ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga actgcagcag
     4801 ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca agcaatgttc
     4861 aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca tttagatttt
     4921 aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat caaacatgaa
     4981 ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga tatggatttc
     5041 aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac gaagaaacag
     5101 ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg caagagaaga
     5161 atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg gattgagaca
     5221 aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag gcctttggaa
     5281 aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt agccgcacct
     5341 gaagagggaa attttgagga actgacggca cctacactta ttttagctgg aaactgtctt
     5401 cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga tcaattgttt
     5461 ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga aaagcaagga
     5521 tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc
     5581 tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc tggagagaac
     5641 gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa aatcttattg
     5701 atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt atggcaaaat
     5761 gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca cttttctgaa
     5821 gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata tttccatgcg
     5881 ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg tccgaaagga
     5941 aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta caccagaatt
     6001 aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc gaatcctcct
     6061 ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga gcgtgttgaa
     6121 tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg aaaatcaaaa
     6181 taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt
     6241 acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct ttacacaaag
     6301 agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg taaaaccagg
     6361 gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc agcgagcaaa
     6421 agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta tccaaaatta
     6481 tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac tgctctctca
     6541 tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag gacatggtga
     6601 tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt tccttgccta
     6661 ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt attatacaag
     6721 tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa aagctatttt
     6781 tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga tttattatta
     6841 tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa cttctggttc
     6901 tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac cagcattcgg
     6961 taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg aaaaactgga
     7021 tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc gattcagtga
     7081 aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg cagagatcaa
     7141 agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg cagttttcca
     7201 accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag cacttgattt
     7261 agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac aaggaaacgt
     7321 taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa
     7381 tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg ctggagatgt
     7441 tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa atgtactgta
     7501 atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc
     7561 aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc tgcggtaata
     7621 agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt attatttgat
     7681 gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc aagggagcag
     7741 aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa actggagggt
     7801 ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg aaatcgagga
     7861 aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc tgtatttggg
     7921 attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa aaacagaata
     7981 tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg caatttcaaa
     8041 gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta ttgaaccggt
     8101 tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata agatgaagga
     8161 tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt
     8221 cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa gtgaaggtga
     8281 attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat gcaaaagttt
     8341 atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg gctctttggg
     8401 tatttccttt gcaattagca acttttatca ataattttcc actaggcttt gtgggtctgt
     8461 ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag aatccgagct
     8521 tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta gcggttactc
     8581 ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact ttgatgttca
     8641 tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt ggaagagcag
     8701 gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc attttgagaa
     8761 gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca gggattacag
     8821 cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca gcacatagtg
     8881 gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg ttcttggcgt
     8941 ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt tggggctata
     9001 ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga
     9061 atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat tattactcgt
     9121 tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt
     9181 caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga tgtttgaaaa
     9241 tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac
     9301 ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac ctggcgaatt
     9361 caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac agtattacga
     9421 attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag tagacaaaga
     9481 tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag ctagtaaaga
     9541 agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata caccagaaca
     9601 tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga agggactagc
     9661 cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa
     9721 gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg aaatgaagca
     9781 aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat cgaaaaaatt
     9841 ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga agtatgaagg
     9901 gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc agttcctttc
     9961 taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg aagtcgttca
    10021 tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa tgatgggcga
    10081 caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga aaatttatgt
    10141 cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca ctgatccgac
    10201 tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc gctatactgg
    10261 aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg atacgaatga
    10321 aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata tccaatcaga
    10381 tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg aactcttttt
    10441 tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat tgagccaaga
    10501 actggcagaa aaaaaccaag aagatctttt ccggaaaatg gaactgccat tgtctatcat
    10561 tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac aagaaatgaa
    10621 gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag aagccggcga
    10681 agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa aaatgggatt
    10741 acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac tggaacagct
    10801 tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg caaaaattca
    10861 atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag tgcatacccg
    10921 ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta atttgcagaa
    10981 catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc ctagagaaaa
    11041 agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac ttgcgcatat
    11101 ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc atgccagcac
    11161 agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca
    11221 ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac tttcacaaaa
    11281 tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg aaaaataccc
    11341 aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag gctacgtcga
    11401 aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt acaatatccg
    11461 ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg ctgatatttt
    11521 gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc aagctacgat
    11581 gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt
    11641 agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac cactgatcac
    11701 tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta agacagagca
    11761 atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa
    11821 ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag
    11881 atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga aaccatccaa
    11941 tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga actgatctca
    12001 cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat cgataaacat
    12061 acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga tgtacgaaaa
    12121 tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa
    12181 ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa tggactgaaa
    12241 aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac tggtctaggt
    12301 aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca accggcgaac
    12361 acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt tttggatcgt
    12421 gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg agaatcaggc
    12481 ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat acgttgtgga
    12541 acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc gaattgtcaa
    12601 aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata gctaccggta
    12661 aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat ggcgatgtga
    12721 ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta tcagtgtttg
    12781 gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg atgattgttt
    12841 ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt atccgaaaag
    12901 cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt gtagatatcc
    12961 cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc gtttatacta
    13021 cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta gaacaagcgc
    13081 agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt
    13141 tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg ctttttgcta
    13201 atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga aagtgaggga
    13261 tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa
    13321 tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag
    13381 cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac cagcaggcag
    13441 aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca cctgatcgta
    13501 gtaaaacacc gctcccagaa agtgaccaca ttcccgatga gcaaaaaatc gaggaacagc
    13561 gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg
    13621 acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag atgactaaat
    13681 cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa aattgaaatt
    13741 tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct tatatccgtg
    13801 ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca actagttgta
    13861 aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa tgagatgtcc
    13921 cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg atggacgtgc
    13981 gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat ttgaacgaat
    14041 tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt tcaatcgcga
    14101 taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta tggaacaaat
    14161 ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa acgaagtatc
    14221 gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg aaatcgctta
    14281 tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct taaaagagtt
    14341 gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag aaaacaatta
    14401 agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa ccaaaaaata
    14461 tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc ttgctgtatc
    14521 tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg ttatcagaaa
    14581 tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt agtagtctag
    14641 gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc ggcttactgg
    14701 atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag ctgttagaac
    14761 cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt ctggaaaaag
    14821 ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg catacagcag
    14881 agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat gaagcagtgt
    14941 acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa tctgaatcag
    15001 tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat tggctggcaa
    15061 aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg tatcatcagc
    15121 tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat gactttagtg
    15181 aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag agacgtccgg
    15241 tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc tcgtcaaacg
    15301 aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt ccaccgatga
    15361 aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa gaaaactggt
    15421 tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat gttttgctga
    15481 attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta aatacagtgg
    15541 caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag cacattcggc
    15601 aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat caaactggca
    15661 agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa gatgaaaaac
    15721 agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac ctggcaaaaa
    15781 aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca tcagcaaacg
    15841 caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg cggatgttca
    15901 agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa aaagttacgc
    15961 taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc caacgatgat
    16021 cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg tcacttatgt
    16081 accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc gagtaaaagc
    16141 aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac gtacagatgg
    16201 acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg ctgatccaaa
    16261 agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa catatttatt
    16321 aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag tccattttcc
    16381 gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag aaaagttaga
    16441 tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg cgatgagtag
    16501 ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg aacagttacc
    16561 aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt ccgtcactca
    16621 acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac gttatttgac
    16681 aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg acaagtccaa
    16741 ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac agaaatgacc
    16801 ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt tatgagggct
    16861 attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg cggtagcgca
    16921 gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa aacaagcagt
    16981 acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtatcac tcaagtagaa
    17041 gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga agatccagcg
    17101 aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc tgaaaaaata
    17161 agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt agttgagatg
    17221 ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat tgatgtttca
    17281 ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt atagaacgaa
    17341 aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct tattttattt
    17401 ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat ttatctcagc
    17461 gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac attggatcag
    17521 atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat gcagttgctg
    17581 gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa gatggattag
    17641 aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca caagatggta
    17701 ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc ctagaagctg
    17761 gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca aacatcggag
    17821 agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca gaaaaaatac
    17881 ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa aaaccagtta
    17941 cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa agtgagatct
    18001 tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa cagctcaaac
    18061 aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa atgattgagg
    18121 aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg aaagctattg
    18181 tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa cattggataa
    18241 gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg aagaagcaat
    18301 caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc ctgtaaaacg
    18361 gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac atgatattgc
    18421 cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg actggttttg
    18481 tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga ttcattataa
    18541 aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag tattagctgc
    18601 agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga ttggacacgg
    18661 tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga agacagaaca
    18721 agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac aagtgattca
    18781 agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg ctatagatga
    18841 accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa tttctataga
    18901 ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa tagtccagcc
    18961 aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg gtgaaaaatt
    19021 ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata tctattatca
    19081 tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc attatattga
    19141 atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag aagaaaattg
    19201 ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta attaaaagga
    19261 gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa aacagaaaca
    19321 cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc ttttgcgcaa
    19381 gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa tgaagataac
    19441 ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc cattggattt
    19501 atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca aatcattgaa
    19561 aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac attgaataca
    19621 aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac agatggcagt
    19681 acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc aatggagatc
    19741 ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg tcctgatgaa
    19801 gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga ttttgcttat
    19861 acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc tgctcaagca
    19921 aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac aatgatcaat
    19981 gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt tccagaaaaa
    20041 acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc tgaagaagct
    20101 tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg caaagcacaa
    20161 atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat caaagtagat
    20221 atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag catcgtggaa
    20281 ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc agttcgtgga
    20341 ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct atttgccgga
    20401 ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga aaaagcaaca
    20461 gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa aagaacagct
    20521 tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa agggcaggac
    20581 aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa agaatggaaa
    20641 agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa ttagcacgct
    20701 ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta gctgaaactg
    20761 gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa gctgatactt
    20821 tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc caaaaaacat
    20881 tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt gcacgtgttc
    20941 ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc aatgtacgga
    21001 aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca atcggaatct
    21061 tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc tcgtggatct
    21121 ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg gatttagaaa
    21181 cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg tctaactccg
    21241 tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa tttcatgtaa
    21301 cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga atcatgagag
    21361 gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg aaaatccaag
    21421 cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat cttccagttg
    21481 aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa gaaaaagctt
    21541 agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt acacaatcct
    21601 ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg gtatgataaa
    21661 catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt tgtcagcctg
    21721 ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa aactcggaaa
    21781 aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct catttatttt
    21841 ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca ttctgaatct
    21901 gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat catttgcaga
    21961 tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg ttatattagg
    22021 gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac ctttcgcgcc
    22081 gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt accgaatcgt
    22141 ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa aaggaatgaa
    22201 gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac tggcagtgat
    22261 cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta atggtccatc
    22321 aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg cctttttatt
    22381 ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga ataagttatt
    22441 taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct actctttaag
    22501 ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt atctatggaa
    22561 tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg aaaaccatcc
    22621 taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc tttcaggggt
    22681 tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat tattcagacc
    22741 agttgttgga acagttg
//
LOCUS       DANKAY010000042        22704 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000042 DANKAY010000000
VERSION     DANKAY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..400
                     /locus_tag="PWY98_001549"
     CDS             <1..400
                     /locus_tag="PWY98_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2037305.1"
                     /translation="QEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEMHLVD
                     PEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGKT
                     AEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            393..1094
                     /locus_tag="PWY98_001550"
     CDS             393..1094
                     /locus_tag="PWY98_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2037306.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1094..2680
                     /locus_tag="PWY98_001551"
     CDS             1094..2680
                     /locus_tag="PWY98_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2037307.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2699..2920
                     /locus_tag="PWY98_001552"
     CDS             2699..2920
                     /locus_tag="PWY98_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2037308.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2917..3678
                     /locus_tag="PWY98_001553"
     CDS             2917..3678
                     /locus_tag="PWY98_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2037309.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4058..4567
                     /locus_tag="PWY98_001554"
     CDS             4058..4567
                     /locus_tag="PWY98_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2037310.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4642..5181)
                     /locus_tag="PWY98_001555"
     CDS             complement(4642..5181)
                     /locus_tag="PWY98_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2037311.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5321..5932)
                     /gene="rpsD"
                     /locus_tag="PWY98_001556"
     CDS             complement(5321..5932)
                     /gene="rpsD"
                     /locus_tag="PWY98_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2037312.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6194..7045
                     /locus_tag="PWY98_001557"
     CDS             6194..7045
                     /locus_tag="PWY98_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2037313.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7073..7708
                     /locus_tag="PWY98_001558"
     CDS             7073..7708
                     /locus_tag="PWY98_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2037314.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7728..8501
                     /locus_tag="PWY98_001559"
     CDS             7728..8501
                     /locus_tag="PWY98_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2037315.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8909..9706
                     /locus_tag="PWY98_001560"
     CDS             8909..9706
                     /locus_tag="PWY98_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2037316.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9684..10097
                     /locus_tag="PWY98_001561"
     CDS             9684..10097
                     /locus_tag="PWY98_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2037317.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10081..12726
                     /gene="mutS"
                     /locus_tag="PWY98_001562"
     CDS             10081..12726
                     /gene="mutS"
                     /locus_tag="PWY98_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2037318.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12744..14852
                     /gene="mutL"
                     /locus_tag="PWY98_001563"
     CDS             12744..14852
                     /gene="mutL"
                     /locus_tag="PWY98_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2037319.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14874..15434
                     /gene="maf"
                     /locus_tag="PWY98_001564"
     CDS             14874..15434
                     /gene="maf"
                     /locus_tag="PWY98_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2037320.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15519..17507)
                     /gene="treP"
                     /locus_tag="PWY98_001565"
     CDS             complement(15519..17507)
                     /gene="treP"
                     /locus_tag="PWY98_001565"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2037321.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17730..18443
                     /gene="treR"
                     /locus_tag="PWY98_001566"
     CDS             17730..18443
                     /gene="treR"
                     /locus_tag="PWY98_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2037322.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18520..18966
                     /gene="msrB"
                     /locus_tag="PWY98_001567"
     CDS             18520..18966
                     /gene="msrB"
                     /locus_tag="PWY98_001567"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2037323.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19136..19738
                     /gene="ruvA"
                     /locus_tag="PWY98_001568"
     CDS             19136..19738
                     /gene="ruvA"
                     /locus_tag="PWY98_001568"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2037324.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19751..20752
                     /gene="ruvB"
                     /locus_tag="PWY98_001569"
     CDS             19751..20752
                     /gene="ruvB"
                     /locus_tag="PWY98_001569"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2037325.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20781..21359
                     /locus_tag="PWY98_001570"
     CDS             20781..21359
                     /locus_tag="PWY98_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2037326.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21411..21893
                     /locus_tag="PWY98_001571"
     CDS             21411..21893
                     /locus_tag="PWY98_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2037327.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22010..22384
                     /locus_tag="PWY98_001572"
     CDS             22010..22384
                     /locus_tag="PWY98_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037328.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7385 a         4068 c         4914 g         6337 t
ORIGIN      
        1 tcaagagttt ttcattatta ttttcttttc tgtattagga gaaggcatac ggatttttgc
       61 tcatttacct attcctggaa gtatcttagg tattcttttt ttatttttag catttgaaat
      121 gcatcttgtt gatcctgaaa aaatcggagc aactggtgat tttttattga ataatctagc
      181 cattttattt gttcctgctg gtgtaggttt attggaatat tttgatgata ttgcttcgat
      241 atggcctgtt ttactgggag cagtcgtcgt gtgttccatc gtcacgatgg ttgcagcggg
      301 aaaaacggca gaaggtgtag aagctttgct gggctatgtc cgtaagaaaa aagcagaaag
      361 ggtatctgta aacgaaatgg aggagaatca aattgactga gttttttgtg aatccattat
      421 ttggcttaag tttgacttta ggagtgtacg tattatctga caaatttttg cggaaatata
      481 aactgcctgt tttgaatcct ctcgtattat cgattgttgt tattatctta tttttgcatt
      541 tttctgggat cagctataaa gactataata tcgggggcaa ttttttgact atgctgatta
      601 ctcctgcgac agtggcactt gccatccctc tttataaaaa cttccgttta ctgaaagaga
      661 atttttatcc tgtaatggct gcgattttag caggaatagt cgcaaattgc ttggttagtg
      721 tagggttcgg ttacttcttc tctcttcata aagagatggt gatctcatta ctacctaaat
      781 ctgtaacgac ggctatttct gtcgatttat ctcatacgat gggcggggtg aatgccgtga
      841 cacttgctat tgttgtctct acaggtattt ttggttcgct gatcgccact catattttcc
      901 gcctattcaa gatcgaaagt ccagttgctc gcggtgtggc gttaggaagt acttctcatg
      961 ctattggtac agcaaaagca atcgagattg gtgaaataga aggaatcatt tccggacttg
     1021 cgatatgcgt caacggaata ttgaccgttt tattgctacc gttattcttt cagccttttg
     1081 ctggcttgtt ttgatgcgaa aaccactatt ttatgctaca ataaccaaaa cgttcgagca
     1141 taaaaaagga gggaaagacg tgtatttgtc ttccgagtat cttaaacaat ttgataaaga
     1201 tctatattat aagattctcc tcctagaaag ttttgaggat caagcttggc ataccgctgc
     1261 acaattagcg caagttgttc agctggatgc ccgcagcgtg tcgaaatacc tgaacgagct
     1321 gtctaaaaac tatcagcagt ttagcggtaa gacacaccct ttgtttacaa aaaatcaccg
     1381 ttctggttat aacttttatg atacgctcga ttcaattgaa catgagcgtt ttttgatcta
     1441 tctcgtgcaa agtacgctga aattccagtt gctgcacgac attttttttg aagaattcca
     1501 tacaatgtac caatttgcgc aaaagcatta cattagtgaa tctactgctc atcgaaaaat
     1561 caatgaatgg aaacagcaac tgcaaactta cggaattcgg ctgcaacgag gtacatatat
     1621 cgctcaaggc gaagaagaaa tcattcgact ttacttgcat atgacattct ggcagttgtt
     1681 tcgagggaaa atctggccat ttgaaacgat ttctcaaatg gacgtaaaga atatggcaga
     1741 acatatcatg gcttttttta atgttcgtct gaacgagatc aaaaaaagac gattggaata
     1801 tatgctaggt gccttttttt taagaaaatc acagaagcat tatgttgtat tgaatgagaa
     1861 aaaaagacgg ctgatttctg acaatctgtt gttccagcga ttttgccagg tcatggagcc
     1921 ggtatttcct aattattttc aagtagaaga cgagttaggc gcattatttc ttgtactgat
     1981 gactcgtgaa gaatattata gtgatcctaa gatacggaag aaaatttttg attttcatca
     2041 acaagcaaaa acaccgccct ttacagccct ttcagaagca aaagccgctc tttctcttta
     2101 tcaggaagag caggggctgc cagcagaaaa tctaaccttc gaggcagaaa actatttatt
     2161 cagcagtcat tttttcgcct atctttttcc taatgcgaaa gaaacgattg atggcaatag
     2221 tagtgatttt attaatcatt tagttattga aaacaaggaa ttgaaacaat ggctcgtaca
     2281 tttttttgaa agtagacaca aacatcccaa tcatctggca tttaaaaatc atgcgttttt
     2341 gatggggcgg tatctgactg tgtttaaaac attaggagcc tttactccac agcttccaaa
     2401 gatcacgatc cttttaatga cagatttccc attatttgaa gagcaactgt tagaggaggg
     2461 attacggaca tttttccgta atgagtatca gctgattttt ctgccaacag attatcgggg
     2521 gagagaagtt gatcttctga tttcgacaag taaagtccac cgcaaaccat gggcggatct
     2581 tgactatttt attgtgactg aagaactaaa attaattgat tacatccaac tatctcaaaa
     2641 atttgaaatg attcaaaaac aaaaactatt aaaacaataa caaagaagga gaaaaaagat
     2701 gacatttgaa gaaattttac cagaattgaa aaaaggtgca aagattattc gtaaaggatg
     2761 gagtggattt gaactttatg ttgttctcgt atccgatgat gaatttgatg gctgtccggt
     2821 gacgccgtac ttattgatca aaacaagtga cgaaggtttt tccagcttcg ctcctacagt
     2881 ttgtgatatt ttagcagatg attgggcgat cgtagaatga aaaaccagta tccagatcta
     2941 gctaaacagg ttgtatttat cacaggagct gcttctggaa ttggtgctgc gcaagcccga
     3001 gcgtttcttg aacaagatgc ttttgtattt ggattagatc aaaagttcgg taaaatggaa
     3061 gaaatttctt tgcgatttcc tgaaactttt gcctacgcac taggtgatgt tcgacaaatg
     3121 aaagaactcc aacaagcggt atcgaaatgc caaaatcatt ttggtgaagt tacgatttta
     3181 ctgaatacag cggggatatt ggatgcctac aaacccttga tggaaactga cgaatcgctg
     3241 tgggatctga tttatgagac gaatgtgaaa agcatgtatc agctgacaaa aatcatcctt
     3301 cctgatatga tccgccaaaa aagcggaacg atcatcaata tggcttcgat tgctggcttg
     3361 attgctggtg gaggtgggat cgcttataca agcgcgaaac atgcgattgt cggatttacc
     3421 aaacaactcg ctctggatta cgcaggacag ggtatccgag taaaaggaat tgctccagga
     3481 gccatccaaa caccaatgaa tgcggctgat tttgaaggga aaggtgagat ggcagagtgg
     3541 gtggcaaatg aaacaccagt caagcgatgg gcacagccgg aagaagtagc tgagctgacg
     3601 ttatttcttg cctctcctca agcaagctac atacaaggaa cgatcatccc aatcgacggc
     3661 ggatggttgt taaaataacc ttgtgttttt ttcaagcttc tgtatgataa aactaaagag
     3721 agaaacaacg gttgatgata aaaaaagtcg atgactgttc ccgacattcg atgccgaaca
     3781 gccagttcaa aaaattcttt ttaataatcg ttaatattcg aaactaagcg gcttttcacg
     3841 acctcttgat ataaacgata ttcaaaagct tgcaactgcg gtaacaagtc cagccaaatg
     3901 aaaaatataa ggagctattt ttatttgacc attcttatta cttgttgcag ggcagctttt
     3961 tcactacctt ctgcgacact tcagttggaa ttttcttccc atcctttggt cgcggtcatt
     4021 accaagataa aattatcttg caaaggagaa atagaacatg gaaaaaaata atacccgttc
     4081 agccaaacgc tggacaacga cagatgtgac tcggatggcg ctagtcactg gtttatatgt
     4141 ggcagtgacg ctggttttgt ctgtctttag ctttggagtg attcagattc gtctctcgga
     4201 gatgttcaac tatctgtcct tgtataacaa aagatacatc gtcgcggtaa cagcgggagt
     4261 agctttggct aatattgctt ctcctcttgg actgatagat gttgcagtag gaagcgtcag
     4321 cacgctgctt gtattgctta tcaactataa aatcactagt cgcatcaaaa acatgaaaat
     4381 caaaatggcc gtgacagcat gcgtatttgc tttttccatg tttacagtag ctggtcagct
     4441 aacgatttta tatcaactac cttttttctt gaattggtgg gtgattgctt taggagaatt
     4501 ggcatcaatg attgtcggtg ggataattat ttattggatc ggtaaaaaag tggatttgtc
     4561 aaaataaaga tctgtttaat ttaaaatagg gcagcatgga agagactgaa tgttgagtct
     4621 tcttccatgc tgcttttttg attaatttgt atgaagaaat cgatttagcc gttttttcat
     4681 caagataccg atataagctg ctccagttgc cttgattgct tccggcagta aaaacggcag
     4741 tagtccactg gcaaatgcag ttgtaaaagt aagatcacca cttattttca gccagatcgt
     4801 tccgcagatc aatgcagcaa gagagcccat tagattggca aagattgcat ataagtaatg
     4861 agcatccgtt ctttcgatca tccaaccagt tatccacgtt tgtaagagaa atccaatcaa
     4921 atatccgcca gttggcccaa ataaaacagc tatacctcca gtcatccctg aaaatacagg
     4981 aagaccaatc aagccaagta aaagataaac tccaacagat atcaatccta gttttcttcc
     5041 gagaaaagtt gctgttaatc caatagcaaa tgtttgccct gtcaaaggaa ccaaaccaaa
     5101 aggaatggtc atctgagaaa ggacagaaat aatcgcggca aattccgcag caagtatcaa
     5161 ttctttcaat gttcggttca taacgccctc ttttcgttaa cttaatattt ttatttggtt
     5221 aacgaaattg taaaacaaag tgattacttt gtaaatagaa aatataaaaa aagaccagct
     5281 atccaataga tagcggtctt tttcaagaaa gcttttgaaa ttataagctt tggttgtagt
     5341 attcaacgac aagagcttcg tcgatttctg gatatagttc gtcacgttct ggtaaacgag
     5401 tcaatgaacc ttccaatttt tcagcgtcaa agcttacgaa tgctgggcgt ccaacagttg
     5461 cttctacagc ttcttttact gttacgatgt tttgtgattt ttcacgaact gagatcactt
     5521 gacccacttc tacgtggtat gaagggatat caacgcgttt gccatcaact aaaatgtgac
     5581 catggttaac taattgacgt gcttgacgac gagtcgttgc aagtcctaaa cggtaaacaa
     5641 cattgtctaa acgttgttct agtaagatca tgaagttaac accatgttta ccttctttga
     5701 ttttgctagc ttttacgaac aagttacgga attgacgttc gttcattccg tacatgtgac
     5761 gaagtttttg tttttcagtt aattgcatgc cgtattctga ttgtttgcca cggctattag
     5821 gtccatgttg acctggtgcg taagggcgac gtgctaattc ttttcctgtg cctgacaatg
     5881 agatgcctaa gcgacgagaa attttccatg atggtcctgt ataacgagac attaaaattt
     5941 cctccaatga atataagttt ttggagtaaa ataatccgtt gaaaaattca taatcatata
     6001 gtttgttctt caatcttcac ctttgcagcc gcggttacac aatcgaaccc gagacggggc
     6061 aacaaactag ggatgagtta ttatttttct gctgcattat tttacacaaa ttacagtata
     6121 tgatagatca tccacgaatg tcaagataat tagtacataa aaacgcttta caaacctagg
     6181 ctgtctttat aaaatgagag ggtatgtgca aatggaaaaa agcgaaggaa gtttaaggag
     6241 aaaatcaatg aatccggtat caccattatt tgagaaaatc gatgcttcaa ttgaaaagaa
     6301 ggcgaatctt tttgaaaaca gcttttcacg ttacgctgtt cgagcggttc tcgcctgttt
     6361 gtttttgact ttaggaacgg ctgtggcatt tgcgatcgcg atgaaggggg aagatatcgc
     6421 gcatggtcta ggaaagattt tatacgcatt tatgttcagc tggggtctgg tcatgattct
     6481 atacatgaac gccgaattag gaacatcgaa tatgctttat atgaccgtgg gtgtttttcg
     6541 gcagagactt gctttaagta aggcagccaa gattctattt gcctgtattt ttttcaatat
     6601 gattggcggt atattatgtg gatttctaat ttctatgact acgccatttc aagatttaac
     6661 gttggacaac tttatgttag aatcaatagc agtaaaacta gcaaaacctt cagcaacgat
     6721 tttggtggaa gggatgtttg cgaatatcgt tgtcaatacc gctgtactga tcagcatgcg
     6781 gatgaaagag gatgtcggaa aagtgatcac agtcgtattc atcattttca tttttgcttt
     6841 tttaggatat gaacacgtga tagcgaattt tcctgccttt acgcttactt actttgcttc
     6901 tcacggacag atggatggaa tgacagtcag caatgtgtta cataatttgt tttttgcttt
     6961 ggcaggaaat tatattggtg gcggtttagt aatgggactg ggatatgctt ggctgaatca
     7021 gtcaaaatca tcctatgtgg actaaaaagt cgttcattaa aggggaagtg ccatggaagt
     7081 tttagttttt ccaaaaaatg aggaaaaagc gttacaaatg gaaaagtaca tgaaaaatct
     7141 tttttccttt gctggagtac ctaagccgga acgcgctctt ttacaacaac cgttactgga
     7201 tgagagcaaa aatttatcta tttctgaagt tttagctttt gttcattatt attatgaaca
     7261 aatcgaaaga gagtatcagt atgtagcgat cgatttagtc cagaaaaaca tcaaacggct
     7321 tacatttgaa gacttagtcg tattgttgcc actagttcag aaaaaagaat ggtgggacag
     7381 catcgactct tggagaaaag tatacagcac atggtgtaaa gcctacccag agcagcgtga
     7441 agaagtcttt caattgtttg aaaaaaagga agatttttgg ctacgtcgaa tagcaatcac
     7501 gttgcaatta ggttataaac agcaaacaga cacaggacta ttgatccgag caatagacga
     7561 agatcgtcaa acaaaggaat tctttatcca aaaagcaatt ggttgggcat tacgcgatta
     7621 tagtaagacc gatgccgatt gggtaaaaag acagctgaca acagatttga gtaagttagc
     7681 tgttcgagag gcaagtaagt atttataaga aagcgagacg acaagagatg agacgcaaat
     7741 tatttgcttt tgatattgat ggaacgttgt tagattcaaa caaacaagct ttagatagca
     7801 caagagaagc attggaaaaa ttacgcaaag ccggtcatct ggtcacgatt gctacagggc
     7861 ggagccgatt tcatgcgcaa gagatcattc gcgatcttga ttttacgaac tacattttat
     7921 gtaatggagc ggccggtttt ttagaccatg aacaagtata taagaattta ctagatcgcg
     7981 accagttgga tgcgttcgtt caagaagcgc atcatgagca gatcgataca gcttttgtca
     8041 gcttagacaa tatcaagcgt tcttcttcca atgatgtaac ggtgatggaa gaagcgatgc
     8101 attctttcgg tgctgtcttg cctgaattag atcagttttt tgcagaggaa caagaaatat
     8161 accaagcgtt agcatttttc gatcaaacat atgatgggaa gttctcttca tatgataaaa
     8221 ttcgatttgt ccgctggcat gagaacagtg tagatgtcgt acctaaaaat ggttctaaag
     8281 cagcgacaat tctgcactta gcagaacaag taggcatccc tcatgaagac atcatcagct
     8341 ttggcgatgg acaaaacgac cgtgaaatgt tgcgcatgtc aggaattggg atagctatgg
     8401 ggaatgctgt gccagaagta caagcagaag ctaagatggt cactgataca aacgatcagg
     8461 atggaatttg gaaggcattg aaagagttaa gcgtgattta aaagaggttg tgaaaaagct
     8521 gttctgcatc aagtaataag cccagccaaa caaaaatagc tcctcctatt tttcgttttt
     8581 ctgggcttat taccgcagat gcaagctttc gaacaccgtt taaggaagag gtcgtgacag
     8641 aagtggtcag tttcaagaaa taagaaggga tttacgaaaa ttgcttctca aatttttgta
     8701 aaattacggc ttatttctga agaagtagat gaaacaacgt ttattcggga aatgggactg
     8761 tgacaagatg tttgtcatag tcccattttt ttattttatt tttcaaaact aagaaatacc
     8821 aggaaagagt tccttttgtt tgaccttttc tttcttattg aaaacaacta taatatagag
     8881 tgatgttaaa agagaagagg aatttagtgt gcgggtattg tttattggtg atgtagtagg
     8941 ttcaatggga cgagagatga ttactgaata tcttcctaga ttgaagaaga agtaccgtcc
     9001 gcaagtaacg attgtaaatg gtgagaatgc cgcttctgga cgtggaatca cagaaaaaat
     9061 ctataaaaaa tttttgcaag acggcgtaga tgtcgtgacg atggggaatc atacgtggga
     9121 caaccgcgga atctttgagt ttgtaaatga agctaaaaag atggttcgtc cggcaaattt
     9181 tccggaaggg acgcctggac aagggatggt ctttgtcaaa gtgaaccaag tcgaactggc
     9241 agtgatcaac atgcaggctc gttcttttat ggtggacttg gatgatcctt ttcgtaaaat
     9301 gaatgagctg gtagaagaag ccagaaaacg tacgccgatc atttttgtag attttcatgg
     9361 agaaacaaca agtgaaaaac aagcaatggg ctggttttta gatgggaaag tttcagcagt
     9421 ggtcggtaca catacacacg ttcagacaaa tgatgcgcga attttgccaa gaggaacagc
     9481 gtatctgaca gacgttggga tgacaggtcc ctatgatggt attttgggta tgcgcagaga
     9541 acctgtaatc gaaaagtttc tgactgcatt accaaaacgt tttgaagttg tggaacaagg
     9601 ccgatcgatt ttaagcggct gtatcttgga attggatgat acgacaggtc atgcaaaaga
     9661 aatccagctg attcagatca acgatgatcg accgttcatg gagtagaaag agaggcagtt
     9721 ttttgcagca ggagccggaa attcaaaaag aagtaaggaa actgacaaaa cttttaagag
     9781 agaatgaaac gatcattcgc tacaaagaac ttgaagaaaa gatccagcaa aaccagtatt
     9841 tagctgagtt gagagaaaaa atcaaacaag cgcaaaaaga tgctgttcat tttgcccact
     9901 atgacaaacc agcagcagaa aaagaagcaa tcaaacaagc ggatcaattc atgcaagaat
     9961 ttgatcagca tccgcttgtt gtagcttata gaaaacagct gttggaagca gatgatctgc
    10021 ttcatcattt aacaactatg atacaagaag aaatcaatgg acaaatagag gaggaaaaac
    10081 atgcctcaaa aaactaaaaa tactcctatg atggaacaat atctaagcat caaggcccaa
    10141 taccaagatg cttttttgtt ctatcgttta ggagactttt atgaactatt ttacgaagat
    10201 gcgatcaaag cgtcacagct tttggaatta acgttgacga gcagaaatcg aaatgcagaa
    10261 gaacctattc ctatgtgcgg tgtacctcac catgcagcac aaggatatat cgatgccttg
    10321 attgaaaaag gctataaagt agcgatttgc gagcaggtag aagatccaaa gacgactaaa
    10381 gggatggtca agcgcgaagt gatccagcta gtcactcctg gtacagtcat ggacagcaaa
    10441 ggattgtcgg caaaagacaa caatttttta acagctgtag ttagtcaagg aaatgaattt
    10501 ggctttgctt atgccgattt aagtacggga gaattgaaga cggctcggtt gcatgatgaa
    10561 gaagctgttt taaatgaagc ctctgcattg cagacaaaag aactcgtatt aggcagtgag
    10621 atcactgata cccttcgtga acagctaagc gggcgtttag ggctagtctt ttctcaacaa
    10681 aatgagatgg aagaaaacgc ggaatttagc tttttgacaa gcgaactagt cgatccgctg
    10741 gaaaaagaag caaccggcaa actattgact tatttatcgg tgacacaaaa gcggagttta
    10801 gctcatatcc aaaaagcagt agaataccaa ccagatcatt ttttgaaaat ggattattat
    10861 tcaaaattca acctagaatt aagccaatct attcggactg ggaaaaagca cggcactctt
    10921 ctatggttgt tagatgaaac aaaaacagca atgggtggcc gtttactaaa acaatggcta
    10981 gaccgtccgc ttatccaggc aaaacaaatt aaagcaagac aagaaatggt cgcttctttg
    11041 ctagacagct attttgaacg tatcgatttg cagtcagcat taacgaaagt ctatgatctg
    11101 gaacgcctag caggtcgagt agctttcggt aatgtcaatg ggcgagactt gatccagctg
    11161 aaaacttctt tagaacaagt gccaatgatc agagggttga ttgaaggaat cgatcaaggt
    11221 caatggcaaa gtctgctttc agaaatgcag ccgatggacc atctcgtcca attgatcgac
    11281 caagctatcc aagatgatcc gccgttacaa ataacggaag gaaacatcat caaagatgga
    11341 ttcaatgagc agctcgatat gtaccgttct gctatgagaa acggaaagaa atggttggca
    11401 gaacttgaag caaaagagcg tcaagaaaca ggaatcaaga acttaaaaat cggatttaac
    11461 cgcgttttcg gttattatat agaaattaca aaagcaaatc ttggaaatgc agaactggag
    11521 aagtatgaac gaaaacaaac tttagcaaat gcagagcgtt tcatcacacc agaactaaaa
    11581 gaattggaga cacaaatcct cgaagcagaa gaaaaatctg ttgatttaga atatcagttg
    11641 tttttagctg tgagagaaga agtcaaaaag gctatccagc cgctacaagt acttgcaaaa
    11701 gcaatcagtg cagtagatgt gctacaaagt tttgcaacga tcagcgaacg ctatcagtat
    11761 gttcggccag aacttgtcag tgacaagcat cagcttctga tcaaagacgg acgacatcct
    11821 gtagtagaaa aagtattagg acatcaggaa tatatcccta acagcgtaga aatggcagaa
    11881 gacgaaatga tcttgctgat tacaggaccg aatatgtctg gtaaaagtac gtatatgcga
    11941 cagttggcat taactgttct gatggcgcaa atgggctgtt tcgttccggc aaaacaagca
    12001 gttttaccta tttttgatcg gatttttacc cgaattggtg caagtgacga cttaattgct
    12061 ggacaaagta cttttatggt agagatgatg gaagccaatc aagccctgcg ttatgctacg
    12121 ccaaacagtt tgattttgtt tgacgaacta gggcgaggaa cagcaaccta tgacggaatg
    12181 gcgttggccc aagcaatcat cgaatatatc catcgtcatg tacaagcaaa aacactcttt
    12241 tctactcatt atcatgaact gacggtactt gagaaagaac ttccgcagct taaaaatgtc
    12301 catgttggag ccgttgaaaa agacggagaa gttgtcttct tgcataaaat gatggatgga
    12361 cctgcggata aaagctacgg catacatgtg gcaaagatcg caggattgcc ttccaatcta
    12421 ctagaacgag cagcagatat cctgcatacg ctagaaaatg gcgataacgg acaccatact
    12481 gttccagctg aagtagaaga gaaaactaaa acagccaaac aggagcaaat aaaaccacct
    12541 gcagaaaaag taaatcaaga ccatatacaa gaccgtgtaa aagaagaaaa ccaacagctt
    12601 tctttattca gtgaattatc cgaaaatgaa acagaagtca ttgaatcact gaagcaaata
    12661 aatttactag aaatgacgcc aatggatgca ctaaacacat tatatgaatt acaaaaacaa
    12721 ctgtaggaag aaggcggata tcgatggcca aaatccaaga attatctgaa cgtttagcta
    12781 atcagatcgc tgcgggagaa gtagtggagc gaccagcttc cgttgtgaaa gaattagtgg
    12841 aaaatgcaat tgacgccggc agtactcaga ttgatatctt agttgaagaa gcgggactgc
    12901 ggacgatcca agtgattgat aacggagaag gcattttggc agacgacgta gagaatgcct
    12961 tcaagcggca tgcgacaagt aagatccata atcgcgacga tctttttcgc atacgaacgc
    13021 tgggctttcg aggggaagct ttgccaagta ttgcctctgt ttctgaaatg actctggaaa
    13081 cggcgaatca ggaagaaaaa caaggcacct atctgtcgct gaaaggcgga gaagttattg
    13141 agcatcgacc agcaccactg cgtcaaggaa cgaagattac cgtcagcaat ctgtttttca
    13201 atacgccagc ccgcttgaaa tacgtgaaaa cactgcacac agaattagcg aacatcgggg
    13261 acatcgtcaa tcgactggca ttgagtcatc catcgattgc ttttcgttta gtacatgatg
    13321 gaaacaaaat gctggcaact gctggaaatg gcgatctgaa acaaacaatt gcgggaatct
    13381 acggattatc taccgcaaaa aaaatgctga aaatcgaagg aaaagatcta gattttgagt
    13441 tatttggcta tgtttcattg cctgaggtga ctcgtgccag taggaattac ttatcgacga
    13501 tcatcaatgg acgttttatc aagaactttt ccttgaataa agcaatcgtt gcaggttatg
    13561 ggtcaaaatt aatggtcggt cgtttcccta ttgctgtatt ggaaatcaaa atggacccgc
    13621 ttttagtaga tgtaaatgtt catccgacga agcaggaagt ccggctgtcg aaggaaaaag
    13681 aactaacaga gctaattagt aacgccattc aaaaagcatt gtctcaagaa aatctgattc
    13741 caagtgcagc ggataatcta cggtttaaga aaaaagtacc tgatcagcct aaaacagaac
    13801 aaatcgaaat cgatttgtcc tacgcttttg aagaagaaaa acctaaaaag ccaagtagtt
    13861 tgaattttga tcgagagaca ggtacctttt tcgtagaaga actaaagaac acatttgttg
    13921 acagaattgt tgataagtct gtggaaaatg ttgaaaacta ttctggtttt tctgaagaaa
    13981 acaccactta tccagaaaaa acagcgaaaa cggtggataa gtttggtgaa aatgttgata
    14041 actttgtaga aaaaacagtg gaaaaatctg aaaagaaaga atttcccatt gtggataaca
    14101 tggagaaaac aaatgaagaa caagcagata aatccgtaga agaggatgaa aagatttacg
    14161 aggaagaatt gacactccat cctgaatttg atttcactca aggaaacgac aaaacattag
    14221 aacaagccat caagaaatta gaaacggaaa aaccagtaca acgttttcct cagttggaat
    14281 attttggaca gatgcacgga acgtatcttt ttgcccaaag tagcgatggg ttgtacatca
    14341 ttgatcagca tgctgcccaa gagcgcatta agtatgaata tttccgggaa aaaattgggg
    14401 aagtatctga tgatctgcaa gaattgcttg ttccatttgt gttagactat ccaaacagtg
    14461 atgctttgaa attaaaagag caaaaacact tgctagaaga tgcagggatt tatttagaag
    14521 aatttggtca aaacagcttt attgtacggg cccatcctac ttggtatccc aatggagaag
    14581 aagaatcgat tattcgggag atgatcgata tgctgctgac aactggaagt gtgagtgtaa
    14641 aaaaattccg tgaagcaaca gcaatcatga tgagttgcaa acgatcgatt aaagccaacc
    14701 attatttgaa tgaagcacaa gcacgtgtgc tgctgaagga tctggcacga tgcgaaaatc
    14761 ctttcaattg tccccatggc cgtccagtat tgattcactt taccaattcg gatatggaaa
    14821 ggatgttcaa acgaatccaa gacccgcatt aagtcaaaga gaagagggaa aacatgaaga
    14881 ttgtattagc atcccaatca ccaagaagaa aagagttatt ggctcatatc gtttcagaat
    14941 tcgagattga acctgctgat atcgatgaaa cgccatttcc agacgaacag cccgtcgatt
    15001 atgttcgtag aatggctgaa gaaaaagcac gaactgtctg ggaacaagat gaaaaagaag
    15061 accatttagt gattgccagt gacacgactg ttgttctcaa tcaagagatc atgggtaaac
    15121 cagaagatct ggctgaggct gaattcatgc tgaaaaaact aagcggggaa acacatactg
    15181 tatatacagc tgttgtttta aaaacaaagg aacgagaaga acggatacta gcagaagcac
    15241 atgtcacttt ttatcctttg acagacgaag agatcaaacg ctacttggag acgggggatt
    15301 atgcggataa ggcaggggca tacggcatcc aaaatgctgc gagtgtattc gtcaaagaaa
    15361 tttctggaga ttattacagt atcgtaggat ttccgattgg tgctgtcaat caggcactga
    15421 aaacattcat ttaaacaaaa aagcggacta tctgattttc gattgaaaat ccatgatagc
    15481 ccgttttttt tattgaaaag attacttatc tactctgact aagattttac ttcagcttta
    15541 ccgactgctt cgcccgggcg aacaagtcct gattggtcaa gagaaagtcc tgcaactttt
    15601 tcattattgg tcaaagcaac aataatgtca gttttcttgc cagcttgttc tatttctggc
    15661 aaatccattt cagcaatcag ggtctctggt gtgacaaggt atccttcttt cacgaataca
    15721 ttgaaaggaa caccctttag ttcaacagta tctaatccca tgtgaacaag tacttccagc
    15781 ccctcattag aaagaatacc aatggcatgt tttgtttcaa agacactcgt cactttaccg
    15841 tttactggtg catagacttt gccgtttgac ggttcgacag cataaccgtc acccatcatt
    15901 ttttgtgaga aaactgggtc atttacttca gttatttcct tgatggtccc tgcagcgact
    15961 gcgaataatg tttcttttgt tgcaactgcc gtaccagttg ctgaaacagg ttcgaaagac
    16021 tgtggcgttg ctgttgcgcc atttgctgtt tgaagttgta catcagcagc attttcagga
    16081 acagcaggat caatcttatt caaaattccc tgacgtctaa agatgatcgt caagccaaat
    16141 ggaatgatga cagcaatgat cattgcgatg atgaaaggta cccatgtttc tgcttggata
    16201 gctagtattc ctggcaaacc gcctactccg atcgcgttag ctcgaacacc catcaagtta
    16261 gcaaacatgc cagctaatcc agagccgacc attgcagcga cgaaaggata gacgtatttc
    16321 aaattgattc cgaacatagc aggttcagtt acgcctaagt aacaagaaat cattgctgga
    16381 atcgagattt gttcttcttt tttattccca cgatgtaaga agatgatggc taagactgca
    16441 gagccttggg cgatatttga taaagcaatc atcggccata aattcgttga tccgaaatct
    16501 gcaatcagct gcatatcgat ggcattggtc atatggtgga gaccagtaat cactaaagga
    16561 gcatacagga atccgaatac ggcactaaac aaccagctga tagaagacgt caatccagta
    16621 tttacgatat ttgagatcca gcttccgact gtccagccga taggacctaa aataacgtgt
    16681 gcagctaaaa cagttggtaa caatgaaaac aatggaacaa aaatcatcga gatcgactga
    16741 gggatatatt tgcggaagaa gatttctaga tacgctaaca taaaccctgc cagcatggct
    16801 ggaataactt gcgcttgata accaatcatc tggacttgag caaaaccaaa atcccaaaat
    16861 ggaatatctg ctgcggctgt ggaagctaca ctataagcat ttaataattg cggagaaact
    16921 aacgtaattc ctaaaacgat tcctaatatt tgtgtagtac ccatcttttt tgcaatactc
    16981 caagtgatac ctactggaag aaaatggaaa attgcttcac cgggtaacca caaaaaggca
    17041 ttgacaccat tccaaaactt cgagtgttca acgattgttc caccaagact ttcaaactgg
    17101 ataccttcaa gtacatttct gaatccaagg atcaatccac cgacaatgat tgcaggaatc
    17161 agtggtgtaa agatttctgc taaaacggca atagcacgtt gaacgggatg aaggttttgt
    17221 tttgcagctg cttttccttg ttctttggaa accccttcaa ctccagatac tgcaacgaag
    17281 tcgttataaa aagtagaaac atcgtttcct atgatgactt ggaactgtcc tgcattcgtg
    17341 aacatccctt ttacgctagg tatatcttcg atcgcttttt cgtccgcttt tcctggatca
    17401 ttcaggacaa aacgcatacg agtcgcacaa tgactaactg cagcaatatt ttcttttccg
    17461 ccgatatcat gtaacagctt ctcggcatct gcttgatatt ttgccatatc aataaacccc
    17521 tttctcttaa cttgtctatg caagttgttt cttcatttac tatacttttt ttcggagaga
    17581 attgcaagcg ttttattaaa aagtacgaga gaaaatgata tcgttttaaa aagcttaaaa
    17641 taagcttttc ttaaaaatag gaacattgaa atacaaaaag agctgtagta tagtgaagaa
    17701 atgatggaag aacgagtgag ggaaaaaaca tgaataagtt caatgaaatt tttttagatt
    17761 tggaacagaa aattttagcg aaagaatatc ctccgcattc gcttttaccc agtgagaacc
    17821 aattgatcaa aatttatggt gtttcacgag aaacaattag aaaagcgctg aatcttttga
    17881 ctacggaagg ttatattcaa aaaaaacaag gaaaaggttc gatcgtactg gatcggaatc
    17941 gcttcgactt tcctatttct ggattgacta gctataaaga gctacaggag acccaacgta
    18001 ttccaagtga aacgatcatc catacgttag aagaaacaga agtgacaaaa gcattgaacg
    18061 agatcactgg ctgggagatc ggtgcgcctg tttggcattt gatacgcgaa agaaaaatca
    18121 atggcgaagt agttatatta gacaccgatt atctattaaa ggaaatcgtt cctattttaa
    18181 ctccagcaca agcacaagga tcgatctatg aatattttga aaatgagctt tctttgacga
    18241 ttgcttacgc gcaaaaggag atcacagtag aagaagtaac ggaaatcatg aaaacagcga
    18301 tggatcttca cgatgacact tatgcagtag tagtcagaag cctcgttcat ctggaagata
    18361 cccgctgttt cgagtattct gagtcggttc accgattaga taaattccgt ttcgttgagt
    18421 ttgcccgacg ccgaaaagtg taaatagttc aaaagaaagc aaattgttgg catctgtcca
    18481 ctttggcgct atgataggat cacaaagggg gcatttttaa tggataaacc aataagacca
    18541 gaaaaagaag aactgaaaga aaagttatca cccatggctt atgcagtcac acaagaaaat
    18601 gcaacagaac gaccatttag cgggaaatat gatgattttt acgaaaaggg gatctatgta
    18661 gatatcgtca gcggtgaacc gttattcagc tctgcagaaa aatatgatgc aggttgtggc
    18721 tggccatcat tcagcaaacc aataacgaaa agacaagtgc gcgaaaaagc cgatttttct
    18781 catggcatgc atcgaataga agttcgaagc aaagaagcag attctcatct cggacatgtg
    18841 tttaccgatg gaccagtaga tcaaggtggt ctacgttact gcatcaattc tgcctcactg
    18901 aaattcattc catatgaaaa gatggatgaa ttaggttatg gggaattcaa atcacttgta
    18961 gaataatgga aatacacaaa gagcggtaca atacggggat ttactcgatc gtccgctctt
    19021 tttttattca agcgattgct atttgaaaga aacgctccct agactccttt tttcacaagg
    19081 tgaaatgtgc tacaataaag ggaacgtatg tgtgcaaaag aaagaggact agaatatgta
    19141 tgaatatctg acaggtgtgg ttacctttat caacccctat tacatagtag tagaaaccaa
    19201 tggtatcggc taccagatcg cactaggaaa tccttaccgc tacagtagca aactaaatca
    19261 aaaaataaaa atttatgtcc atcaggtaat acgtgaagat gcgcatcttc tgtatggatt
    19321 tgattcgcta gaagaaaagc aactgttttt gcgattagta agtgtttcag gaatcggtcc
    19381 gaaaagtgcc cttgcaatca tggcaagcga agaccactcc ggtttgattc aagcagtaga
    19441 atccagtgat gtcacttatt tgacgaaatt tccaggagta gggaaaaaga cagcacaaca
    19501 aatgatcttg gatctaaaag gaaaattcgg tgagctgagt atcgatacac cttttagtct
    19561 atttgatgaa gcaaacacaa aagacgctac agctctatct gaagcaatgg aagctctctc
    19621 ggctcttggt tacagtgatc gagaaatcaa acgtgtagaa aaacaattaa aagaagtaga
    19681 taatcagaca acggatgagt atctacgcca agcactcaag ctaatgatga aaaaataagg
    19741 aggagaccaa atgacagatg aatacttgtt atcaccagaa tctggcgaaa atgaactgag
    19801 tttggaaaaa tctcttcgac cacagctgct ttctcaatat atcggacaga gaaaagtcaa
    19861 acaagaactt cagatttaca tcgaagctgc cagaaatcgg gaagaacctt tagatcatgt
    19921 tctgttgtac gggcctcctg gattaggaaa aacgacgatg gccatggtca ttgctaatga
    19981 gatgaccgta aatattcgta cgacgagtgg ccctgctatc gaacgtccgg gtgacttagt
    20041 agcaatcttg aatgaactgg aagcagggga tgtcctgttt atcgatgaga tccatcgatt
    20101 acctagagtg gtagaagaga tgctttattc agctatggaa gatttctatg tagatatcat
    20161 ggtaggacaa gggccaaccg cacatccggt tcatttccct cttcctccct ttactttgat
    20221 tggcgcgacg acacgggcag gtatgctttc tgcaccttta cgagatcgat ttggcatcat
    20281 ttctcatatg gaatattatg acgaacagga tctgcaggag atcgttttac gttcttcgga
    20341 tatttttcaa acagagatca ttgaagaagg ggcattggaa attgctcgcc gatcacgagg
    20401 aacgcctaga atcgctaatc gcttgctcaa acgggttcgt gattttgcgc aagtccaagg
    20461 agacgggaaa atcgatcgtc ctatagcgga tcaggcactt actttgctac aggtcgatca
    20521 ggctggattg gattatgttg accagaaatt gttgcgaaca atgatcgagt tatatggcgg
    20581 cggaccagta gggttaagta cgctttcagt aaacatagga gaagagcgtg agacggtaga
    20641 agatatgtat gaaccgtatc tgatccaaaa aggattttta aagcgtacac ctagagggcg
    20701 aattgctact gcttatgcct atgaacattt tggttacgac tatcatatat gatttttgct
    20761 tttagattgg aggaaaatga atgagtgata caacacgtac gaaacaattg atcaaaaacg
    20821 tattgattga tttgtgccaa acgcaatctt ttcgaaaaat cagcgtccaa aaaatcgcaa
    20881 acgaagcaaa aatcaatcga caaacatttt attatcattt tacagataaa tacgatttgc
    20941 ttcgttgggt ctattgtgag gatgcattgc attatttaga tccacaatgt ctttcacttg
    21001 ataattggga agagcaagca ttgaaaatgc tgaaagcaat tgctgagaag aaagaatttt
    21061 atgcaaatac tgtgctttct gacccagaaa ttttacaaaa agagttttct caactgatcc
    21121 aaaaatcatt tatcaagatt tttgaacaaa tggatgaaga aagacaactt tctgcaagag
    21181 acaaactatt ttatgctcgt tttttttctt acggttgcag tggtgtatta gtcaattgga
    21241 tcacgaatga atatgaagaa acaccgatgg aaatcgctat gcagcttttc cgtttagcca
    21301 aggatacgga attatattct tatcgtcttt atgcaaaaga agagttggaa gaacagtaat
    21361 agaaaaattt tttgttggaa aagcaataat cgctaaaagg agatggggaa atgacaaaaa
    21421 ttttatttgt ttgtttggga aatatttgcc gttcaccgat ggcagaaggc ttgctgagaa
    21481 aacgaatagc attagaagga agggaagcag aattttttgt ggattctgct gctacaagca
    21541 cgtatgaagt tggaaagaca ccgcaccctg gaacgaaaaa aatattaaac caagaaaaag
    21601 tagatatgac gaatatggtt gctcggcaaa tcacacccca cgattttgaa acttttgatt
    21661 ggatcattgg catggatcaa gaaaatgtag aagaactgaa acgaagagca ccacgctcag
    21721 cgcaggggaa aatccatttg tttttaagta gcattcctgg aaaagaaaaa gaaaatgttc
    21781 ccgacccata ctataccaat aactttgatg agacatatca cttgatcaca gaaggcctga
    21841 atcattggtg gaatatttgg attgatgaaa ataaaataac aaacaatttg taaagttaac
    21901 gctttctttt taaggcgatg ttagagcatt tccttgtttt tttgtaaaat gttaggtaaa
    21961 ataaaattaa ttaagtaaca ctttcttagc taaaggagga gtgcattcga tgaacgaaga
    22021 gacagtatcg ccaatgaaga agcttacgaa gttagttact catacttcgt atcaactttt
    22081 tgattctttt gtaaatgaca ctgaagaaca tgctaagaat gtacgattgc aaggaaagtt
    22141 tattcttgag ctgcaaaaag ctgctactga acatagtcta gttatcttac aagtaagagc
    22201 aaatcgtttg agtagcaagt atgaaacgat ctcaggctgg gtggcgacaa aaacggtcga
    22261 aaaagaccga gtgatgatcc gcctacaaaa cgaagagcag cagatccgga tggttcctgt
    22321 agaacaaatt gaaaaaataa caatcctttc accagcaggg gaacaggaac ggttatcaaa
    22381 ataatttata ccagatagaa aagcacgtat gagcttgtac catcaatcat tactaatcaa
    22441 aaataaccga atgctttaag ctgatatcag taaaacggaa cgttttagga tgagtatcac
    22501 agcatgacag cattgggttg attagttgaa tcgattggat ggtacaagtt tttttgataa
    22561 aaaaaggtcc tgctagccgg ggagctagca ggataaggag ttaaaaatga aaaagtgttg
    22621 ttagggttgt ttgttggtat gcttatataa tagtcactag ttgtgaatat tttgtgacga
    22681 aagttttcga aagttgttaa gttt
//
LOCUS       DANKAY010000043        22637 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000043 DANKAY010000000
VERSION     DANKAY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            133..3660
                     /locus_tag="PWY98_001573"
     CDS             133..3660
                     /locus_tag="PWY98_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2037329.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="PWY98_001574"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="PWY98_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2037330.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="PWY98_001575"
     CDS             7448..7789
                     /locus_tag="PWY98_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037331.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="PWY98_001576"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="PWY98_001576"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2037332.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="PWY98_001577"
     CDS             9146..11566
                     /locus_tag="PWY98_001577"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2037333.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWY98_001578"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWY98_001578"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2037334.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="PWY98_001579"
     CDS             13000..13737
                     /locus_tag="PWY98_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2037335.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="PWY98_001580"
     CDS             13751..14566
                     /locus_tag="PWY98_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2037336.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="PWY98_001581"
     CDS             14579..15226
                     /locus_tag="PWY98_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2037337.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="PWY98_001582"
     CDS             15242..15898
                     /locus_tag="PWY98_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2037338.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="PWY98_001583"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="PWY98_001583"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2037339.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="PWY98_001584"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="PWY98_001584"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2037340.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="PWY98_001585"
     CDS             18240..18758
                     /locus_tag="PWY98_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2037341.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="PWY98_001586"
     CDS             18865..19365
                     /locus_tag="PWY98_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2037342.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="PWY98_001587"
     CDS             complement(19645..20565)
                     /locus_tag="PWY98_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2037343.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="PWY98_001588"
     CDS             20730..21743
                     /locus_tag="PWY98_001588"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2037344.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWY98_001589"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWY98_001589"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2037345.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7537 a         3949 c         4821 g         6330 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaat
//
LOCUS       DANKAY010000044        22615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000044 DANKAY010000000
VERSION     DANKAY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..133
                     /locus_tag="PWY98_001590"
     CDS             <1..133
                     /locus_tag="PWY98_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL2037346.1"
                     /translation="RLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLFKKCN"
     gene            209..1456
                     /locus_tag="PWY98_001591"
     CDS             209..1456
                     /locus_tag="PWY98_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2037347.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1471..2223
                     /locus_tag="PWY98_001592"
     CDS             1471..2223
                     /locus_tag="PWY98_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037348.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2926..3141)
                     /locus_tag="PWY98_001593"
     CDS             complement(2926..3141)
                     /locus_tag="PWY98_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL2037349.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3160..3396)
                     /locus_tag="PWY98_001594"
     CDS             complement(3160..3396)
                     /locus_tag="PWY98_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL2037350.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3654..5807
                     /locus_tag="PWY98_001595"
     CDS             3654..5807
                     /locus_tag="PWY98_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL2037351.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5776..7191
                     /locus_tag="PWY98_001596"
     CDS             5776..7191
                     /locus_tag="PWY98_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL2037352.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            7224..8258
                     /gene="sppA"
                     /locus_tag="PWY98_001597"
     CDS             7224..8258
                     /gene="sppA"
                     /locus_tag="PWY98_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2037353.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            8260..8781
                     /locus_tag="PWY98_001598"
     CDS             8260..8781
                     /locus_tag="PWY98_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2037354.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8778..9164
                     /locus_tag="PWY98_001599"
     CDS             8778..9164
                     /locus_tag="PWY98_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2037355.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9660..9732)
                     /locus_tag="PWY98_001600"
     tRNA            complement(9660..9732)
                     /locus_tag="PWY98_001600"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9697..9699),aa:Lys,seq:ctt)
     gene            10113..10682
                     /locus_tag="PWY98_001601"
     CDS             10113..10682
                     /locus_tag="PWY98_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2037356.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10669..11529
                     /locus_tag="PWY98_001602"
     CDS             10669..11529
                     /locus_tag="PWY98_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2037357.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11719..12423
                     /locus_tag="PWY98_001603"
     CDS             11719..12423
                     /locus_tag="PWY98_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037358.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12428..14263
                     /gene="walK"
                     /locus_tag="PWY98_001604"
     CDS             12428..14263
                     /gene="walK"
                     /locus_tag="PWY98_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2037359.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14260..15576
                     /locus_tag="PWY98_001605"
     CDS             14260..15576
                     /locus_tag="PWY98_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037360.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15577..16446
                     /locus_tag="PWY98_001606"
     CDS             15577..16446
                     /locus_tag="PWY98_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2037361.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16501..17310
                     /locus_tag="PWY98_001607"
     CDS             16501..17310
                     /locus_tag="PWY98_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2037362.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17413..18042)
                     /locus_tag="PWY98_001608"
     CDS             complement(17413..18042)
                     /locus_tag="PWY98_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2037363.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18212..19504
                     /locus_tag="PWY98_001609"
     CDS             18212..19504
                     /locus_tag="PWY98_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2037364.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19580..20242)
                     /locus_tag="PWY98_001610"
     CDS             complement(19580..20242)
                     /locus_tag="PWY98_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2037365.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20467..20751
                     /gene="groES"
                     /locus_tag="PWY98_001611"
     CDS             20467..20751
                     /gene="groES"
                     /locus_tag="PWY98_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2037366.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20802..22427
                     /gene="groL"
                     /locus_tag="PWY98_001612"
     CDS             20802..22427
                     /gene="groL"
                     /locus_tag="PWY98_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2037367.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7739 a         3599 c         4500 g         6777 t
ORIGIN      
        1 tagactaaat gctaaacaat gttcagatca agagcttaaa aaaattaaag gaggtgctgg
       61 aacaaaacct caaggaaaac cggcatcaaa tttagtggaa tgtgtttttt cattatttaa
      121 aaagtgtaac taaaattttt tttatgatat cctaaaatca ggaggggatc tcttgattac
      181 tatcgataca aaagcttatc tattaagaat gttcgtggga aactttttct tttttctctt
      241 attttatatg actaatactg atcttaacaa aagaaaatat ctttttgtat gtttattgat
      301 accactcact ttttttgttg aattatttac tgatgtttcc gcagctatac ctataatcgg
      361 aagttatttt atattaaagg aaaagaagaa aactaatata gtgcttttaa ataaattact
      421 attatgtatg cttattgatg atttcagatc tattattact tccatggtta tgacgtatat
      481 gttttcattt gaagggataa aagggtatgg ttatgtagta ttccagataa tgcttgattc
      541 tttgtttttg ctgatgttta tttggttata tagaaaatta gctataaata ggattgttga
      601 acagtccagt tctaaactta ctgctatatt catgatttat ttacttacaa ttggtttatt
      661 tgtttcttat gttgctcatc aatatcaagt atttgatcat tttgtgtttg gtgtcttaat
      721 atttttaatc attcaaatgt ttgttgttct atttttattt attagaataa ctattaggca
      781 acgagaacaa tatgaacagc aattgaaaag aaaagaattg gtttatctaa aaaaatatac
      841 tgattcactg gaaaaagatc aagaaaaatt tgccagattt agacatgact ataaaaattt
      901 attgctgagt ttaaaagaaa tcgctaatca aaaccatgat tcgatattgg aaaaagaaat
      961 aaaagaatta gagatctatt ctaattctta tctaaccaat aagtttgaat atcgttattt
     1021 aaaaaatata aaaaatgaat atttgaaaag tttgttgata gcgaagctat accaagcaaa
     1081 taacaataag atttgttgta aattcgaatg tccaagtact attattgaag ttcctgttcc
     1141 aatatttgac tgtattaggg tgcttggaat tattctcgat aatgcaattg aagcatctat
     1201 agagagtcaa gatagagaac tgtctctagc tatttaccga gatgataaac agatagaaat
     1261 ttcgatatcg aatagttgta gagagatact actctctata gatactctaa tgaaaaaagg
     1321 aataacaaca aaaaagggac atcaagggtt aggtttatct agtatagagg aaatcaataa
     1381 aaagaatccc aatatgtttg tgaactatca aaacgatccg tatcaattta ctactcaagt
     1441 tattttgttg ttttgaggga aggaggattt atgagttatc caataatatt atgtgaagat
     1501 caaattatac aattaaatca gttagaacga attattgata atttcattct ttttcacgat
     1561 aaagtattta agatagtatt gaaaacacaa agtccgttag aagttaaaaa atacctcaaa
     1621 caattccgac caaagcaagg aatatatttc ttagatattg atttaaatca tgaagttaac
     1681 ggtatagaat tagcagaagt aatcagaaaa tatgatgttc aagcaaaaat catttttaca
     1741 actactcatg atgagatgtt acccgtaaca ataaaaagaa gagttgaaac gttaggattt
     1801 gtaacaaaag atcaaacact agatgagtat cgaaacgaga ttgttgagtt attgttatta
     1861 gcgcaagaaa ggatagatgc aacaaaacag gttacgaatc aagcatttgt tttttccata
     1921 ggttctcaaa catttacttt tgatataaat gacatttatt ttgtagaagc ttcaaaatta
     1981 ccacatagat tatcattgtg tactaaagat gggcagtacg aattttatgg aagaattagt
     2041 gagcttgaaa agaaatatcc tatgttaaca agaattagtc gagcgtgttt agtaaatatc
     2101 tttaacgtta aagaaataga ttttaaaaaa cgttcactat attttgattc agagttggcc
     2161 agaaacttta ctttagggaa agcacaaaaa attaaggaga agttgaaaga aagcactgtt
     2221 tagtggtttt ttctttaggg agattgcatt tttctaataa aatagattcc ttcactagtg
     2281 caaatttttc tgaatcctat taaagttgta ccaattttta tagctgttat tagttacgtg
     2341 cataacagca atattagtta ctggaggaat atttctatgg tctggaacca aaaaagggcc
     2401 aaatgaatag ttcttttctt gtttttatta agtaccataa aggggaatta aaattaaatg
     2461 tatggtggaa gatgcgaaag atttggcaaa ttctactaat ttttagaaag tgtttttcat
     2521 tttttagtta aaaaatttgt cctgtcaagt gcaatcctat gattgtactt gataggattt
     2581 gagataatta ctttcctgtt tttataaatt taacttgcag gaacaggctt taatgcacat
     2641 ccgacaagta cattaccaat acctcctgta acggccgaaa taggtcctcc tgcagcacca
     2701 actacagcgc tgcaagtcct tttgtaaaaa tatctctacc attacatttc caaagaattt
     2761 ttatctgact ttaataaaag ataaaaattt aatataatta taaaaactag cacaagaagc
     2821 tttagtaaaa agttctgtcc attccaaact tttgaaaaga taaagaacat acctatgttc
     2881 gtaatcgaca caaaccaatc ttgaggtttg atatttttta attgtttacg caccggtcca
     2941 catgctgaat ccagcaccta ataaagctcc acctacacca caagcagcta ttgcataagg
     3001 tcctccggct gaacaaagtt ttactcctgc tgttgcccca gaaaaagtag tgcctaaaac
     3061 tcccacggca ctcccacctt gaatattttt caactcttct gaagataata taaatgttgt
     3121 ttttgtgttt ttaatatgca tactacttat tctccttttt tatttttttc gagcatccca
     3181 gccagctgca gcacctccag cgacagctcc atataaccct ccaacaacac cgcaagctgg
     3241 tccgcaaatt gctgttccta aacctgcacc acctactgcc cctgtggtaa gtccacctac
     3301 aacttgcccc aattttcccc aagcattatt gcctccacca acagtttgtg catttccaca
     3361 atacttagtt cctttacatt ttgcatgttt aaacacgctt ctttaaataa tattttttac
     3421 ggtgctactg taacatacgt atttgattaa cagtaaaaat cttcctgaaa cttatcaagt
     3481 ttattcttga atagtaaaat ttccaaaata aatttgattc aggaatgaaa aagttagtgt
     3541 tcaagaactg ttttcaaagt ttttgtggaa ttatgagaca atattttcac tgtattaaag
     3601 ctatattgag aatttttcta taaaaatttt ttagaaggag ttgtgttaga taagtgataa
     3661 aatcatttcc ttttgtgcag caacaagatg aaaaagattg tggtgttgct tgcctttcga
     3721 tgattttaaa atattttaaa actgatatcc cgattcataa attacgggac ctatctggga
     3781 ctgatttaga gggtacttca gcatttggat tgaaaaagac atttgaaaaa ttgtcttttg
     3841 attgtatggc tattcaagca gatagtgaga tctggaaaga aagagatctt atcttaccat
     3901 taattgcaca tatattggtt gataacagct atatgcacta cgttgtaatt tataaaacga
     3961 agggaaacgt actatggatt gcagatcccg ctaatggaaa aaatagaaaa acgattgaag
     4021 cgtttcaaaa agaatggaca ggtattcttt tattacctat gcctagagaa acttatattc
     4081 ctattaaaga aaaagtagct ggattaagtt cttttttgcc tataatttgg aaacaaaaag
     4141 gattagtatt ccacatcgtt ttagcatcga ttttcattac gttttttggc ataggaagct
     4201 catattattt tcaaggtatt cttgattttt tcatccctaa tcaagcacgt tcaactttaa
     4261 atataatttc cattggcttg atcgctgtct atttatttcg ggtattgttt gaatatagtc
     4321 gtagttatct tcttattatt ttaggacagc gaatgagtat ggcaattatg cttcagtatt
     4381 tcaaacatgt gttaacttta ccaatgaatt tttttgctac gagaaaatca ggggaaatta
     4441 tttctcgttt tcttgatgcc agtaagatta ttgatgcttt agcaagtgct acgttatcaa
     4501 tatttttaga tattggtatg gtgttgcttg ttggggttac gttagctatt cagaatagta
     4561 cgttgttttt aatcactcta tgctctttgc ctttttatgt agttgcaata ttcgctttcg
     4621 tgaaaagcta tgaaaaatcg aacagggaag aaatggcagc cggtgcagct gtaaattcta
     4681 gtatcataga aagtcttaaa ggaatagaaa caattaaagc atatagcggt gaagagaaag
     4741 tatataaccg tatagaccat gaatttgtta aattaatgaa aaaatcattt cgtacggtta
     4801 cgcttgataa tattcaacaa ggaattaaac atttgattca attaattagc agtgcattaa
     4861 ttttatggct aggttcttat tttgttatgg ataatagaat tagtctaggt caacttatca
     4921 catacaatgc tttactagta tttttgacag atcctttaca aaacattatt aatttacaag
     4981 tgaaaatgca aacggctcaa gttgcaaata atcgtttaaa tgaaattttc gcgattgatt
     5041 cagaaaaatc gaaccagtct tttgaatata tgctttctga acaattattt caacaagata
     5101 tcactgtaaa aaacgtttca ttctcgtata gcatgaaggc cccaacgttg aacgaaattt
     5161 catgtacaat tccttcgcac agtaaaacgg ctatagttgg agttagtggt tctggaaaat
     5221 caactcttgc aaagttattt gttcgttttt atgaagcatc agaaggatcg atccattatg
     5281 gaaacattaa tcacttagac attccttttc agatattacg cgatcgagtg acatatttgc
     5341 ctcaagagtc atttttcttt agtggaacaa tcattgagaa tcttatattc ggtttagata
     5401 gcccaccaag tttcgaacaa ataatagaag tttgtgagat agtccaacta aatgaattta
     5461 ttaatcaaca gccattacgt tacgagacat tgttggaaga aggtggaaat aatctttcag
     5521 gagggcaacg acaacgatta gccttagcac gggctttatt aaaaaatccg gacatattaa
     5581 ttttagatga agcaactagt ggattagata ccttgttaga acatgcaatt atgaattatc
     5641 tcctcaattt aaaagaaaaa acgattattt ttattgcgca tcatttacca atagcaaaag
     5701 catgtgatca aattttagtt ctccatgaag ggcatttggt agaaaaagga acgcacgatg
     5761 aacttcgttt tgccgatggt atctatcaaa aattatggga aatctaggag agggaaacaa
     5821 tttatgcaga ataataattg gttcgaacat tctagtatct atactaaaca acatcatagc
     5881 ttctaccgtt gggtgttata ccctgtaatt ctttttttat gtattattgg attgttttta
     5941 acctttgcta aaaaagaggt tgttattcgg acaacggctc aattgaaccc cgaaaaaata
     6001 gagaagttac aagtgccatt agaagctaaa ataatggaaa acttcctaaa agaaaatcaa
     6061 tttgttcata aggacgatgt gttggttaga ttggattgtt ctttaataga gaatgaaaag
     6121 gctcagatag aacaagaaaa ccaacgtatt actcaacaaa taaagatggc tcagctattt
     6181 attgaaagta taagtaaagg aaaaaatttg ttttcaacgg atgacagttt tggctacagt
     6241 aatcaattaa agagcatgtt gtcagaaaaa gaatcactcc gctacgcttt gaagcaaagt
     6301 gaattaaatg atcaaaagca attagaagta tacgaaaaga caaaaagaca actagaaaaa
     6361 caaattgaga gttcagatag taaattacaa gaatggcaac aagtacaggt agcttggagt
     6421 aataatcaat cattaaaaga tttttcaaaa gaaatgatgg caaactatga gaattggcaa
     6481 gaacaactaa ataatgtttc tgatgatcaa aaaaatcaag tgaaactgac aatttcagca
     6541 agcataaatg aacaaattga gcaactaaaa aaagaagtag aacagtatca gtcagaaaaa
     6601 gctaaattag ttaaaccaac tacttctgag aatgacagaa ttagtcaaac ggaaaaagga
     6661 aagcaagagc tagaacaagc aataacaact gtaaaacaaa cagttacaga gctaaatgaa
     6721 aaacaagaga agaaccagtc agttatcaaa agtttggacg atcaactttc aaaaggaata
     6781 ctaaaagctc ctgtatctgg aacggttcat ttaaatgaag aaacaaaggg acaagcagaa
     6841 ctagccaaag gaacagtgct tgcagaaatt tatccgatcc acgaaaaatc aaaaatgaaa
     6901 tttacagcac ttttacctgc gaatgaaagt acctatatta aagaagggat gaaggttcat
     6961 tttaaattgg atcaaaaagg tggggctccg attacgatcg acggaacttt agatgaaatt
     7021 tcagaaaatt ctactgccac tgagaaaggt gttttttatg cagtgaaagg aacgttaaaa
     7081 acaactcaat cgtttccgtt tcgttatgga ttaactggag agttgtcttt aattgtagga
     7141 gaaaaaacat attggcaaca aattgtcgat gtgtttctaa ataaaaaata attattttta
     7201 ctaagaatat aggaggagtt tatatgaata aaagaagatg gattgctgtg ggagtagcgt
     7261 gcggattatt gttactatcc attatcgtcg cgctaattcc aggaaaagat aaagaggaag
     7321 catcaaatac aaccctaaca ggaataaata agatttttta cggatctaac gaaatcacag
     7381 aagaaacgtt agaagaaggc gcatcaaata aaaagattgt caaactatcc gtcaatggag
     7441 ttattgcaga tacaggtgaa agcaatcttt ttagtaggga acaatataac catcagaatt
     7501 ttttaacaca gctaaagaaa attcaagaag acaaagcggt taagggagtg ttattagaag
     7561 taaattcacc tgggggagga atatatgaaa gtgctgagat tgctaaagaa atggcaaaaa
     7621 ttaaaaagct agatattcct atctatactg catttaaaaa tacagcggca agtggagggt
     7681 actacatttc tgctggttca gataaaatat ttgctacgga agaaacaaca actggatcaa
     7741 ttggtgtcat tatttctggt ttaaattatt ctggcttatt agaaaaactt ggcgtaactg
     7801 atgcgacgta taaaagtggt gcattaaaag atatgatgtc acctcaacac aaaccttcag
     7861 aggaagagaa taaagttata caggaatttg tgatgagtgc ttatgatcgt tttgtcaatg
     7921 ttgtagcaaa aggaagaaat atggatacta atgcagtgaa agaacttgcg gatggaagaa
     7981 tttatgatgg taaccaagca gtagaaaatg ggttggtgga tcaaattggt tactctgaag
     8041 atgcattaga ttccctaaaa aaagagaaga aattaactga tgcgacggtt atagaatata
     8101 aaaatgacac aactggcttt gcttcttctt ggttaggtaa taagattgct gaatggcaag
     8161 ggctaaaggc aactacatca gatcgtgtaa tttcagtttt tgaaaaactt ggattttctg
     8221 atactccaaa accaatgtat tactatggag ggctataaaa tgaataagaa gagtggcatt
     8281 gaaactttgc ctaattatgt atttgcggga ttttggttac gattttttgc ttttcttatt
     8341 gatttaattt gtattagcat catcacccat gctactatag gtctctttta caaaatcatg
     8401 ggactggaat ctgacaaatc cattattact ttttatggta tagcgtcatt agctatttat
     8461 ttaacctatt ttgtactttt gactaagctg aattcaggac aaacaattgg gaaaatgatt
     8521 tttgggataa gagtagtgag ctttgaggaa aaagagctaa gttggcaaac tatctttatt
     8581 cgtgagtgta tatgtaggtt tatattaaaa aattttgtat ttcaaattgg gtatttggtg
     8641 gctgctttca cgcccaagaa acaacatatt ggggattatt tttctaacac gtcagtggtt
     8701 gttattcgtt cattagaagc tattaactat ggtcaaaaaa tttctaattc taaaacactt
     8761 aatctggaga gaattaaatg aataagagaa aaacagagtt tattttgatt ttaattagtg
     8821 gaataacagg gattttatgt agtcttttag cattgatggg tggtatgttt ctatcttcta
     8881 ttgattcaac tactttaaat actacagaac taaccagtaa ggatatagat atgttagtca
     8941 atctaagcgg gaatgttgtt ttatttggag gaattaccct agttttttcg ataatgattt
     9001 tcataatagg ttttttagta agaaaaaatc aaaaaacagt ggtctatggt attagtttac
     9061 tcattttatc tattattcca ttttttcttt taacattatt gtgggtaatt cctggagttt
     9121 taggtattat tgcgggattg atgcttctaa taagaaaatc ttaattaggc aatgtgtaga
     9181 caacgtgaaa cgattaatca attaaaagct aaatacgttt tgagaaatga taacttgtag
     9241 tcgatggaag acatgtagat aaaaaaacta cgtgtctttt atttattcta attttttcga
     9301 aaaaaagaca ctctctgatt gttgtattaa tggaaaaggg gaaagcagca agtaaattta
     9361 gtttttctat taaatattct tcacttttta accttttata gtacttagat ttaatcctat
     9421 atgtttagag agttcattgg cagcatgatt ttgcaaagct tctcgaatta ttgttatatt
     9481 agaaacttta ctgttcataa ttatgcttct tttttaactc aaagtccaaa gggctacagt
     9541 ggactttgag ttaaaaataa aaagccctag ttattttgaa aaataaaaaa agaacagcgt
     9601 aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt ccgtgggttt ggcacccaat
     9661 gggccgtgag gggctcgaac ccgcgacccg ctgattaaga gtcagctgct ctaccaactg
     9721 agctaacggc ccgtacaaaa agtacttaat aatagtagca ctaagaattt taaaatgcaa
     9781 gactacattt aaagaaaatc agaaaaatct ttacttcctt ataggaaatc attcatagat
     9841 tattcacatt tgctcgctat tatataagca taccaacaaa caaagttctt gcaagagaac
     9901 aaaccctaac acttttttca ttttttactc ctttcccgcc gtctccccgg cgggtctcct
     9961 gttcttaacg ataaaagaca agaaggctta gccttcttgt tttttgtttt catggaatcc
    10021 cgctaatcaa atcacctagt taaagcaact agattgaatg tttatcataa gtctgatatc
    10081 atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag caattaatgg
    10141 agtgggggaa ggaactagaa acattccgtc tgcctcactg ggaagaactc cctgacttag
    10201 aactttatat ggaccaagtc agaacgttag ttgatcgtta tctttcacct gtgatacaag
    10261 gagaaaagca tccattgctt acctcttcta tggtaaacaa ttacgttaaa ctaggtttga
    10321 taccagcacc agttaaaaaa agatataaca aagaacacgt agcattttta cttgcaatca
    10381 ctacgttgaa acaagtattg actattcctg aaatcaaaga agggatattg ttccaaggaa
    10441 aaacagtagg aattcgtgaa gcctataatc tattttgcga tgaacaagaa gcagctgtgt
    10501 ggatggtcag ccaattagca caaggtaaat ctcatccgca aaaattcgac caggcaaccc
    10561 ctgtagaata tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa cttcttgcgg
    10621 aaaaaacgat tgttctagaa accgaatatt taaaagagga gaaaacaaat gaaaaacaat
    10681 aaaatcgcaa ttttagtaga ttcaggtacg gatgttccag aagaattgat aaaaaaatac
    10741 gagatgtata tgatcccatt gaaaattatt tataaagatc aggtatatac agataaaatt
    10801 gacattaccc ctgaagagat ctatcaacgt ttgccagagg aaatccctgg tacatctttg
    10861 ccggatggtg agtcgatcac agaaatcttt gagcaaatca aaaaagacgg ttatgaaaag
    10921 gtacttgcgg taacaatttc cagtggattg agcgggacat ataatgtggt tcgtttaatt
    10981 gcacaacagc aagaagaatt ggaagcatat gtactagata cgaaaaatat tggtattggt
    11041 gcaggttttt ctgcgatcca agcagcaaaa tggctggaag aaggagtaga atgggatcaa
    11101 ctgatcagta atctgaatga gttagtcgaa agaacaaaag ttttctttaa tgttgcgaca
    11161 ttggaatatc ttcaaaaagg tggacggatc ggtttagttg cttctattgt aggaaccgct
    11221 ttaaagctta acccaatcat ctcatgtaat gaagaaggga tttattacac agtaggaaaa
    11281 gctaggggac gtaaaaaaag tttggataaa acggttgctt acgtcaaaga aaaaatcggt
    11341 aatgcaaagg tcttcaactt agctgttgcc catggggatg cgaaagaaga agcaattgaa
    11401 atggaagcga gacttaaaag agagttccca caagcaaacc aaatctactt tggccagatt
    11461 tctccggcat tagtagtcca cactggtccg ggattattgg gtgtcggtgt acaagtttta
    11521 gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc ttttttattg
    11581 aagttagaac aaaaaataat aaagtatttt gcactttcat tttaattttc tcttttgttt
    11641 tataggcaaa gatgaaacaa aagcagaaat acggtataat gagaaggtat gacaataata
    11701 tataagaggt ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa gccgatttcg
    11761 gatatcgtca aattcaattt agccaaagaa ggctatgacg tttatactgc atatgatggg
    11821 gaagaagcct tggaaaaagt tgctgaggta gaacctgatt tgattttact tgatttgatg
    11881 ctacctaaaa tggacggctt agaagttgct cgtgaggtac gcaaaactta tgatatgccg
    11941 atcattatgg taacagccaa agattctgaa attgataaag tcctaggctt agagttaggc
    12001 gccgatgatt acgtgaccaa gccattttcc aaccgagaat tagttgcacg tgtcaaagca
    12061 aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag aggcagcacc tgcggaattg
    12121 acgattggtg atttgacgat ccatccagaa gcttacatgg tgacgaaacg aggagaaaca
    12181 atcgagttaa cacatcgtga attcgaacta ctcttttact tggcgaagca ccttggacaa
    12241 gtgatgacaa gagaacattt attacaaacg gtttggggtt atgattattt tggagatgta
    12301 cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa tccaagccat
    12361 ccaaattatt tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc agaacaggag
    12421 taattggatg aaaagaaaag tccgcttttt tcgatcagtc aattttaaga tcgctattac
    12481 ctttattctg attttattga tctcgatcga aattattggt gcctacttta tacgtggttt
    12541 agaacgttcc acgatcaata catttattaa agatatgaat caaacggtgg aatcattagc
    12601 aacaacaatc agcccggaat tgaatcgaaa agataatgca gatgatgaag aggtaaatgc
    12661 gaatatcaaa agatttattg aaaacagtgc gactagcgat atcatcgaaa tacgggtcgt
    12721 tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg ctgtgggaaa
    12781 gaaaaatgat tatgttgata tcaatgattt cacgactaaa cgttatgttg ctttagataa
    12841 cgacgataaa cgggtgaata tcaatgttca accgatcctg tccccgaccg gcgatgccgt
    12901 tatcggggct ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa tcaataacac
    12961 agcagtcatt ttctttactg cctcactaat tgcggcattt atttcaatga tcgtatctgt
    13021 tttagttgct cgttcgatta cacagccgat cggtgaaatg agagaacaag ccattcgaat
    13081 cgctagagga gactacagtc ggaaagtcaa agtgcatgga caagatgaac ttggacagct
    13141 ggctgatacc ttcaatcagt tagctgaacg tattgaagaa gctcaagata caatggaagc
    13201 tgaacgcaat cgtttggata gcgtgctatc ccatatgaca gacggcgtca ttgcgacaga
    13261 tcgcaggggt aaagtcatta cgatcaatga tatggctgtt tccttgctag atatcaaaaa
    13321 cgaggaagca atcggacaat cgattttgac acttttagat atcgaagaag aatatacgtt
    13381 gcggaaatta ttagaagatc caaacgaaat ggtacttgac cgttcaacca gtacattgga
    13441 aagtgatcag atgatcttgc gagtagactt ttctatgatc cgtcgcgaat caggttttat
    13501 cagcggatta gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag aacgggaacg
    13561 tcgtgaattc gtctctaatg tttcccatga acttcgtaca ccgttgacaa gtatgcgtag
    13621 ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac caaatttttt
    13681 gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg atcaatgacc tcttaaatct
    13741 atctcgcatg gatacaggta atacccagct tcaattagaa tatgtcaatt tcaacgagtt
    13801 agtgaatttt gttcttgacc ggtttgatat gatggtggga aatcaggaaa aaaattataa
    13861 gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa gtagatacag ataaaatcat
    13921 ccaagtagtc gataatatca tgaataacgc gatcaagtat tcaccagatg ggggaacgat
    13981 tacttgtcgc ttattagaaa cgcataataa cgtgatttta agtatcaccg atcaaggatt
    14041 ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag tagataaagc
    14101 acgtgcaaga gcacaaggtg ggacaggact tggattagct atttctagag aagtgattaa
    14161 ggcgcatcga ggcgctattt gggcagagag caaggaagga aaagggtcga ctttttatat
    14221 ctcgctgcca tatgaacctt atgaggagga atggtgggaa tgagaatctc tgaaaaagtc
    14281 gttcgtatag gactcatttt gatggttgcg ttaagcatat acttctctta tgcaatctgg
    14341 ctgagcccag caggaaaaag ttcaatcaac gtagatgaga caaacactca aatgatcgag
    14401 tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac atggcaccat
    14461 tcgggaatga tccaagaaac caacagtgaa aatctcgtgt accgattgca attgatcatc
    14521 gataaagcaa gatttggaaa gctttctgaa gtagtttcag gagacaaatc acagtttgaa
    14581 caggtgaaaa atctgaactc tggttttgag ttggcctata atgcgccttt tttgctaaga
    14641 gaatacaagg aagcatttga tttagattta gatgtgtcga acttggcaaa agaaagcgaa
    14701 aatatgtatt ttactagaat acaattcgat caaggaaata aaaaaatccg atttttgaat
    14761 tattcgaacc atctcgtata tgaagcaaat atttcgttga attggacaga tatcaataaa
    14821 gaattaacag caaaagacac aaaatggaca gaaatgacaa cagaaccatc gacgatcgac
    14881 acgcaatatg ctacgaaaca ttcgatcaaa ctgaagaagt atagttatat cctgtcacag
    14941 caaccctata cactttttag aaatgctttt ttccagaaac cagatagcgt gaaaaataat
    15001 gatgaaagca atgaattgat tttttacggt gggaatgaaa caatgtctat ccattcggaa
    15061 acgcaagtag ttgattttcg tggagagctg ccagagaaaa aagaaaatgc tagtgtattt
    15121 tctgaaagtt tcccctatat tagcaaatta ggcagttctt tagggaacct cagatatttt
    15181 gaccgcagtg acaatacgat cgattatcgg atattcgtgg aaggcttccc ggtttttggg
    15241 acagacagca gaggacagat tggtgtggaa gttggcggtg aaacgaatgg ttctgtgcaa
    15301 gtgaatatcc aaaccagttt gaatacgatt caagtgccga taccttcaga cgaagaagta
    15361 gaacttgcaa gtacgaatga aatcatccag cagttaaaag ctaagggagc agatagcaac
    15421 aagattggtt cagtgatcgt tggctataca tggcaaaatg tcaaagaaac gaatcgtgtc
    15481 gttgatctct tacctgaatg gtatgtgaaa tataaagata actggtattc agctaacgac
    15541 cttctggaaa aattacctgg attggaggcg aattaattgg atttcaaaaa aatcgaatgg
    15601 atatttttcg tcgctttttt agggttgaat gtgtttttat tcagcagtta tcatgacgcg
    15661 gtataccaag aaaacaatgt cattcgctct aatcagactg aatcaatcca acaacgtctg
    15721 gctagcgacg atatcactta cacggggaag atttcgtctg agaacaagca aggctattat
    15781 cttagcgcgg agcagaccaa tcttagcact tcattagcga attatcgcaa ggctggtaaa
    15841 agcggcttgc tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa atctttatcc
    15901 agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc cttggaagat
    15961 tttttatcta acaaagacaa tattttattc ggtaatgatt atgtgtacac gaaaaacttt
    16021 tctcatttag aagaagaacc actagaaatc caagcaagcc aagagtatga aggcatccca
    16081 ttcaatgatg atacagcaaa aatcgttctt tctttagaaa aaaacgataa tgattatagc
    16141 atcacgaaat acatgcagac gcatttatct gatatcgagc agctgcgcga aaagacggaa
    16201 ctgtatacgg aagaggaagc aatcaataca ttgtacatca ataacaaaat accaagagga
    16261 tcaaaaattt tatggcgtca gctagcttat tcctgtattc tgaaagtccg ggaaaaaaat
    16321 gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt ccaaattgag
    16381 agcgtcaacg ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa agtggaagat
    16441 caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta agaagggaag taggagtcaa
    16501 atggctgaag aaaaaccagc ttttaaaatc agtatcctag caagtggaag ttcgggtaat
    16561 tcactttata tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact tagcggtaaa
    16621 aaaatcactt ccttgatggc tcaaattgat cggacaccgg accaattaga tggaatactt
    16681 gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg tcttggctcg taagtatcat
    16741 ttggatgttt ttgccaatca gcagacttgg gaagcgatgg aaccactgat tggaaatgta
    16801 ccagtggaac aaaaacatct atttgagatg gggaaagtca gaacattcgg cgatatcgat
    16861 attgagagtt tcggcgtctc ccatgacgca gcggctccgc aattttatcg tttccataaa
    16921 gaaaatcggt cattcgtcat gttgacggac accggatatt gcagtgatta tatgcgaggt
    16981 gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc atgatcttga gatgttacgc
    17041 atgggacctt atccgtggaa tttaaaacag cgtattttag gagatagagg acatctttcc
    17101 aacgaagacg gtgcacttgt catgactgat gtgatcggta atcggaccaa acgaatctat
    17161 cttggccatc ttagcaagga aaacaatatg aaagaacttg ctcatttaac aatggaaaat
    17221 gttttgaaag aaaaagattt tggcgttgga catgattttg aaatctatga tacagatcca
    17281 gatgaagcaa ctgagctatt ttcgatttaa acagttcaat caataaaaat ataaaaagca
    17341 gaagataaaa tcaatcaggg ttggttttat cttctatttt tattttttta gcatattaaa
    17401 aaaataaccg tattatcgac tgactacact gatgtgctgc ttttcatgtt tctcatccag
    17461 tatctcatct actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt cgctagcagc
    17521 gtttctttgt aaataatcat cacttaacaa ataagtacct gtacgtttgc catgtggatc
    17581 aaataaagaa gctggactga tataagtcca atgtagattt ttagattgtt tcaattttaa
    17641 caatccttcg tacatattga aagctgttgg atagaagggt gcatttttag gtacttggtc
    17701 aatcatacga gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc caacgacaat
    17761 taagcgtgta tcggttcctt caagcatctc aatcaaatgg gacaaagaag tttgatgcat
    17821 ttcttctttt cctttgggag cgttgaaagc atcaacgaca acatcaaaag atgcaagatc
    17881 agtagttgtc aactcaaata aatccttttg tataaacgga acatctgatg gcaatttttc
    17941 tggatgacgc acgattgccg tcacgtctaa atctctggac aatgcttcgt ctaaaataaa
    18001 tgaacctgca tggccagtag ctccaatgag tgcaattttc ataaaaaacc tcctttttta
    18061 ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt ttgaaaagct
    18121 cagtgcgcat tcctttcatg gttcgtgatc attgggtata gttaaattaa gaaaactgat
    18181 tgataaaaat agaagttgaa ggagataaga aatgaaagag tggttagatc gtgccttttt
    18241 accaatgata acatctgttg tgaatcttag acccttaact gctttgcgta atggaatgac
    18301 ttacacgatc ccaataatta ttgttggctc gctgtttttc ttatttgctc attttcctgt
    18361 ggattacatc gctcagtgga tagacaaaac aggattttct atgtatttta cacaagtata
    18421 ccaggctaca tttgatgtga tagccttttg gatcgttttt gccattgcat acgcttatgc
    18481 aaaagaagaa gacatcgatc gcttaccagt cggcataaca gccttgtctg cttatctact
    18541 gcttattcag ccactaaata ctcagataga tactgtctgg acaggtgctg aagggatact
    18601 aggagcagtt acaactgggc tcatcatcgg atggggctat gcacgtttag taagacttgc
    18661 gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt tggcagtctt ttacttcatt
    18721 gattccaatc attgttatag tcacttttac gcttatcggt tcagcggttt ttcaacattt
    18781 gttcagaatg actgttgttc aaggagtatg gacatatgtc cagcttccca ttcaatcgtt
    18841 catagacacg ttgtttggtg tgcttttatt aggttttttt gttccgtttt tatggttatt
    18901 tggtcttcat ggatctgcga tggtcaatgg gttggttagt cccgtcttgc aagcgaattc
    18961 attagcaaat gcagaaatct tagcctcagg acaagagttg acggtggcaa atggcggaca
    19021 tatcgttact cagcaatttc tcgatcagtt catgacggtt acgggtgctg gattgacgtt
    19081 aggggctgta ttttttatga cggtttttgc gaaatcgaag aaatacagag aattaggaaa
    19141 tcttgctctt ttaccagcct ttttcaacat caatgaatca attatttttt ccacaccgat
    19201 cgtgatgaac ccgatgatgg ctgttccttt tattttcgca ccggtcgttt caggattgat
    19261 tacctattcc gctttatatt ttggattcgt tcctttattt acagcagtcc aagtcccttg
    19321 gacaacaccg ccgatcctct cgggtttctt agtaggcgga gtgcctgcag cgattttaca
    19381 aggtatcgtg ttgtcaatta gttttttcat ttatttccca tttttgaaaa aaatagattc
    19441 tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa agatgaacga
    19501 ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa atcaccaatc taggatgaca
    19561 ggagtattta tagtcatgtt tatactagcc ctttttggac agctaattga acaagaacaa
    19621 ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca gatccgacca gttgctttgt
    19681 actctaggct gaagaagaaa cctaggaaaa agtagattag cagatgacca tcattatgag
    19741 caaacgcaaa cagcaaagag ctgatcactg ctccgaccca aaaatttgaa tatcgactga
    19801 tgatccccag tatactgcgg cggaatacga actcttccat gatgggtcca gcaacagttg
    19861 tcgccaaaat aaaagcgggt gcttccatga tcatctggat gatattttgt gtattctcgg
    19921 aaggggttgc tttgccaaac aatacagatt ctaacatgac agcgatgctt tgcagaatca
    19981 atgcgacaaa gattccaacc aatccataaa taacaatctt tggagcagaa gcttttttta
    20041 ccgctgattc gatcgctaag ggttcacgtt gcttaaaata aaaaatagct aggacaacgg
    20101 cgcctagtat atacatgact gcagttgctg tgatcaattc agacgtagat tttacaagtc
    20161 ctatcgaagc taggatcaag ggtgagataa aaattagacc atagcaaaaa atgctgagga
    20221 agctatattt ttttaaagac atgtaattcc tccttttaag ggataacaag catgcaaggc
    20281 aagcttatat cgattgattt caattatatc ataatttcaa taagcagctg tcagaataaa
    20341 atttcttttc tttgatttaa aagcttgcaa aaaagaggga gaatgagtat tattaaaaat
    20401 gtaggttagc actcgaaggt taagagtgct aacagattaa aaaaagagtt atcggaggga
    20461 tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa
    20521 aaaactgtcg gaggaatcgt tttagcttct gctgctaaag agaaacccca aactgggaca
    20581 gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta
    20641 aaagcaggcg accaagttat gttcgaaaaa tacgccggta cagaagtaaa atacgaagga
    20701 aaagaatatc tgatcgtcgc aggaaaagat atcatggcga ttgttgagta aatttggaag
    20761 ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa gaattgaaat
    20821 ttgcagaaga cgcacgcgca gctatgttac gcggggtaga taaattagca gatacagtga
    20881 aagtaacatt aggacctaaa ggccgaaatg tcgtactaga aaaatcttac ggctctccat
    20941 tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga attagaagat cattttgaaa
    21001 acatgggtgc aaaacttgta tcagaagtag catcaaaaac aaatgatatt gcaggagacg
    21061 gtacaacgac agcaactgtt ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa
    21121 ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga attggcaaca aaagcagcag
    21181 tcgaagaatt gcataatatc tcaactgttg ttgattcaaa agaagccatt gcacaagtag
    21241 cagctgtttc ttcgggttct gataaagttg gacatttgat tgctgatgca atggaaaaag
    21301 ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca gaactagatg
    21361 ttgtagaagg tatgcagttt gatcgtggtt acttatcaca atatatggta acagataacg
    21421 acaaaatgga agctgttcta gaaaacccat atatcttgat cacagacaaa aaaatctcta
    21481 acatccaaga tattctgcca ttgttagaac aaatcttgca acaatcacgt ccattgttga
    21541 ttattgctga cgatgtagat ggtgaagcat tgccaactct tgtattgaac aaaatccgtg
    21601 gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc
    21661 ttgaagatat cgctattttg acaggtggta cagtaattac tgacgatcta ggtttagaat
    21721 tgaaagatgc aacaatcgaa aacttaggaa atgcttctaa agttgttgta gataaagaca
    21781 atacaacgat cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt gtgcaattga
    21841 tcaaaaacca aattgctgaa acaacttctg attttgaccg cgaaaaatta caagaacgct
    21901 tagctaaatt agcaggtggt gtagctgttg ttaaagttgg tgcagctact gaaacagaat
    21961 tgaaagaatt aaaattacgc attgaagatg cattgaatgc tacacgtgcc gcagtagaag
    22021 aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg
    22081 tagaagcaga aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac
    22141 caattcgtca aatcgctgaa aacgctggtt atgaaggatc agtgattgtt gataaattga
    22201 agaacgtgga actaggcact ggtttcaatg ctgctactgg agaatgggta aacatggttg
    22261 aagcaggtat cgtagaccca actaaagtaa cacgttctgc cttgcaaaat gccgcttccg
    22321 tttctgcctt gttattaacg acagaagcag tcgttgcaga taaaccagaa ccagcagcgc
    22381 cagcagctcc tgcaatggat ccatcaatga tgggcggtat gatgtaagaa aaagcaactt
    22441 gcttatctac aactgatcat tagtgaaaga cagataaaaa gaggctggaa cagaagcgtt
    22501 taactccaag aaataagaag aaattcacgg aaattgcttt tcaaattttt gtgaatttca
    22561 gcttatttcc gaaggagttg cttctgcttc cgctgtttat acgtgtttag agcgt
//
LOCUS       DANKAY010000045        22022 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000045 DANKAY010000000
VERSION     DANKAY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PWY98_001613"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PWY98_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2037368.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PWY98_001614"
     CDS             complement(2020..2922)
                     /locus_tag="PWY98_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2037369.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PWY98_001615"
     CDS             complement(2919..3611)
                     /locus_tag="PWY98_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2037370.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PWY98_001616"
     CDS             complement(3654..4295)
                     /locus_tag="PWY98_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2037371.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="PWY98_001617"
     CDS             complement(4333..4647)
                     /locus_tag="PWY98_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037372.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="PWY98_001618"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="PWY98_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="PWY98_001619"
     CDS             complement(5984..6916)
                     /locus_tag="PWY98_001619"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2037373.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="PWY98_001620"
     CDS             complement(6913..7689)
                     /locus_tag="PWY98_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2037374.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWY98_001621"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWY98_001621"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2037375.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWY98_001622"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWY98_001622"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2037376.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="PWY98_001623"
     CDS             complement(12014..13294)
                     /locus_tag="PWY98_001623"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2037377.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="PWY98_001624"
     CDS             complement(13313..14239)
                     /locus_tag="PWY98_001624"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2037378.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="PWY98_001625"
     CDS             complement(14252..15535)
                     /locus_tag="PWY98_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2037379.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWY98_001626"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWY98_001626"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2037380.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="PWY98_001627"
     CDS             complement(16323..17234)
                     /locus_tag="PWY98_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2037381.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="PWY98_001628"
     CDS             complement(17231..17695)
                     /locus_tag="PWY98_001628"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2037382.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="PWY98_001629"
     CDS             complement(17700..18398)
                     /locus_tag="PWY98_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2037383.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="PWY98_001630"
     CDS             complement(18385..18771)
                     /locus_tag="PWY98_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037384.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="PWY98_001631"
     CDS             complement(18918..20588)
                     /locus_tag="PWY98_001631"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2037385.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="PWY98_001632"
     CDS             20771..21004
                     /locus_tag="PWY98_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2037386.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="PWY98_001633"
     CDS             complement(21103..21534)
                     /locus_tag="PWY98_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2037387.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>22022
                     /locus_tag="PWY98_001634"
     CDS             21613..>22022
                     /locus_tag="PWY98_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2037388.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6228 a         4924 c         3889 g         6981 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ct
//
LOCUS       DANKAY010000046        20713 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000046 DANKAY010000000
VERSION     DANKAY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(69..1574)
                     /locus_tag="PWY98_001635"
     CDS             complement(69..1574)
                     /locus_tag="PWY98_001635"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2037389.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1666..2256)
                     /locus_tag="PWY98_001636"
     CDS             complement(1666..2256)
                     /locus_tag="PWY98_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2037390.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2435..4081
                     /locus_tag="PWY98_001637"
     CDS             2435..4081
                     /locus_tag="PWY98_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2037391.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4130..4849
                     /locus_tag="PWY98_001638"
     CDS             4130..4849
                     /locus_tag="PWY98_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2037392.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5328..6275
                     /locus_tag="PWY98_001639"
     CDS             5328..6275
                     /locus_tag="PWY98_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2037393.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6287..7207
                     /locus_tag="PWY98_001640"
     CDS             6287..7207
                     /locus_tag="PWY98_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2037394.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7248..8705
                     /locus_tag="PWY98_001641"
     CDS             7248..8705
                     /locus_tag="PWY98_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2037395.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9050..9664
                     /locus_tag="PWY98_001642"
     CDS             9050..9664
                     /locus_tag="PWY98_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2037396.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9657..11381
                     /locus_tag="PWY98_001643"
     CDS             9657..11381
                     /locus_tag="PWY98_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2037397.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11378..12829
                     /locus_tag="PWY98_001644"
     CDS             11378..12829
                     /locus_tag="PWY98_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037398.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13302..15455)
                     /locus_tag="PWY98_001645"
     CDS             complement(13302..15455)
                     /locus_tag="PWY98_001645"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2037399.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15457..16503)
                     /locus_tag="PWY98_001646"
     CDS             complement(15457..16503)
                     /locus_tag="PWY98_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2037400.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16653..17942
                     /locus_tag="PWY98_001647"
     CDS             16653..17942
                     /locus_tag="PWY98_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2037401.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17955..20657
                     /locus_tag="PWY98_001648"
     CDS             17955..20657
                     /locus_tag="PWY98_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2037402.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         6784 a         3654 c         4177 g         6098 t
ORIGIN      
        1 gaactgtgac aaaaaatcac agtccctttt tttcttagaa atgggaaacc actagtatat
       61 aaataccatt attcttcaat caaccgcttc acgattgcat aatccccttc ataaggttca
      121 tctacaccat tgattttttg atagggatcc acgccttttc ccgcaataac gactgaatct
      181 tgcggagcgc tttctgctaa agcgcgtcga atcgcttcag gacgatccat ttcataatga
      241 acgatcaaat ctggattagt aattgcttca ttgatttctt ccgcgatttt tttcggatct
      301 tcaaatgctg ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga tagcactttt
      361 ccaaaatcat gtcgacggga gattgctttg tttcctgggc tgcctaatac aacaattaca
      421 cggccatctg gatgttcatt tttggcaaat ttcagcaatg ttttcaagct tagataatta
      481 tgtgcatagt ccacatagac atgcgctcca ttggattgga tcaattgatc cattcggcct
      541 ggtacacgag cttcttttaa tccgtcttgt gcatcttgac tggaggcacc tgttagtgct
      601 gcggcaatga tcgcactggc tgcattttct tggttgaatc ctccagccag caaaatatca
      661 taagcagtat cgcagatagc taagcgatct tcatgagaag ataaagtaaa accgtgagta
      721 cctttatctg aacggatcac ttgataattg ctttcttccg aacgaccata agtgatcgtt
      781 ggaatctgga atagatcggc tgtttcccta agtaaatcaa aatagtcact ttcatgattt
      841 aaaacaactg tttttgaatt caagatcaat tgtcgcttgc aatagaaata gtcatcaaat
      901 gtcggatgtt cgatcggact gatatggtca ggcgagatat tcaagaagat tccgacatct
      961 aaagtcaagc cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc catcactaga
     1021 tgagtcatac cattttcaac tgcttctgac atcatacgat ataaatctaa tgactcaggc
     1081 gttgtcaaat gggatttgaa atatgttttg ccatctaacg ttgtattcat agtagaaagc
     1141 aatgctgttt ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta cgcagcagtt
     1201 gttttacctt ttgttccagt gaatccgatc actttcaatt tattttgcgg atagtcgtaa
     1261 aaagccatag caagaagcgc cattgcttta cggatatccg ttacgatgat tcctactgct
     1321 gaattttcgt atggttgttc tgatacataa tattgaatac cctgttcgat tgctgaatcc
     1381 aaatattctt ctttgaaatt cattccttta cagaaaaaca aagtagaagc atcagctttt
     1441 cttgaatcat aacttaactg aagaaattca gctttttctg gtacatcaag atgccagcct
     1501 cttgttgaaa caaattcttt taatagattt tcttttaata gtaaatgacg gatgtcttct
     1561 aaagagaagc tcattgattg atccctcgct ttattcaatt tcttggttta ttatagcaca
     1621 ctctttttaa tatatcgttt gtttccgcta tgacaaaatc cagctttatt caaattttct
     1681 tgacacaggg gacaagcgga tgagtatttt cataaacaat gaactgactg taatagatag
     1741 gagaacgaat agtgtctcca acaatccaaa aacataataa ttcaaaaaga cgataagact
     1801 attggtaagt aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga aaataatcgg
     1861 cggtatcgtt ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa ccccaagact
     1921 gaacaaaaga cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt ccacttttgg
     1981 aacaatctct aaaacaacag gaaaaagcag acttcccgct acagtcttaa aaaatactga
     2041 ttttcctaat aggacagcgg ctagaagcag catcataaga ttgatgctcc aaacagtaac
     2101 tgagcgttgg atagaaaagg ccgtttcaac taaatgtccg atggcgccca ctccggcgga
     2161 agctatatcg tgagggagca agaaccaatt caatccaact gtgataaaga aaagacctaa
     2221 aaatataacg ggtagttttt ttatttcttg tgtcattgat atctcctata aatgttagcc
     2281 ataaaaggct gtatatttct ttactttacc aaaaaaacga aaccgaattc aaaaaaacaa
     2341 ttgttcttat aaaagttata taaaatcgat tgccagtttc tgttttacca attaaaatga
     2401 tagtaatgtt ttgtagaaag ttggaatctg accgatggat aatcaaagaa aaccagcatt
     2461 agatcaatta acaccgcaag aaaaaatggc acgaggctct gcttggatga ccgccagtaa
     2521 tatcatttca agattgctag gcgctgtcta tatcattccc tggtatgctt ggatgggcga
     2581 aaatgctaag gctgccaatg gattattcaa tatgggatac aatatatatg cattgtttct
     2641 actgatctct acagcaggaa tacctgctgc gattgcaaaa caaactgcac gctataattc
     2701 attgaatgaa tatggcactt cacgacgatt atttatccgt gcattacaga tgatgggcgg
     2761 gttaggcctg ttgtttgctt tgttcatgta catcgcctct ccttggctag ctcatgcttc
     2821 tggtggcgga gaagaactga ttccaacgat gcgttctttg agtatcgctg tattggtttt
     2881 tccttgtatg agtgtgatac gagggtattt gcaaggcaat caagaaatga tgccttatgc
     2941 gctgtcgcag attgtagaac aggtggcacg tattttttat atgctattgg ctacattcat
     3001 catcatgaaa gtcatggatg gtgactatgt gacggcggtc acgcaatcaa ctttcgcggc
     3061 atttatcggt atgctagcaa gtattcttgt ccttttgtat tttttgaaga aacatcaagc
     3121 gtatacatcg gcgtttattc actacagcga aaacaaaata aatattgcaa cgaaggaatt
     3181 gttgttagat accgttaaag aagcgattcc gtttattatt gtgggttcag gggtaacgat
     3241 cttcaaatta gtcgatcagt tcacttttat gaagatcatg tctgcatcca ccgagtattc
     3301 caatgcacag ttactggatc ttttttcgat ttttagtgcc aatccagata agttgacgat
     3361 ggtagttatt gcattggcaa catcgatcgc gtctactggg ttaccgttga ttacagaagc
     3421 tgttacgttg aaagatcgag taggtctggc aaaattgaca agcggaaatt tacagttgtt
     3481 ttcatttttt atgtttccgg cgacatttgg tgttatgtta cttgcctatc cgctaaatac
     3541 attattttat acaccggatt ctctaggaag taatgtactg atccaagcaa gttttgtagg
     3601 actcttccta ggactatata tgctagtttc caacatgctg caaggaatgt ttgaaaacaa
     3661 ggcggcaatt aattatttgg ttattgggtt tctagtgaaa ctccttcttc aatacccagc
     3721 cattcgttta tttgaagtat acggcccttt attggcgaca atgatcggat ttgctgtgtc
     3781 ttgtaccttg atcttaaaga gaatccatga agtagcccgc tttaaaccaa gttttgtctg
     3841 gcgcaggaca ttgctgatct ttattcttac cttgattatg cttttggctt cgtggatcac
     3901 aaagatgatt tttggtgctt ttttaaacga agatagcaaa ttccagtctc tattgttgat
     3961 tcttgtagta gcaggtatag gggctttagt gtatggttat ttagctttga aaatccgctt
     4021 ggcagaaaga ctactaggac caggaatagc gcgtttacga cataaattaa aaataaaata
     4081 agtgaatcag aagagctaga ttgatgctct tttgaaatgg gggaaacgca tgcgtttaga
     4141 taagttttta gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat taatcaaaaa
     4201 aggaacgatc gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag taaatgaatt
     4261 cgaagatgcg gttacttatt taggagaaat cattacgtat caaaaagatt tttactacat
     4321 tttgcataaa cctgcaggag tcatttctgc tactcaggat aatcatgatc aaaccgtaat
     4381 cgatttattg gaagaccagg attatcggga agatcttttt ccagtcggcc gattagataa
     4441 agacacagag ggcctcttga ttttaacgaa tgacggggtg tttgcccatc agttactatc
     4501 acctaaaaaa catgtggaga aagaatatct agctgaggtt agcggtataa tgacagaaga
     4561 agatatccgt ttatttgcag aaggactgat tattgatggc gatgagcaga cattgccggc
     4621 aaaactccaa attgatcaag tagatcaaga gacagaaact tcaattatcc gtctgatttt
     4681 gcatgaagga aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa aagaagtctt
     4741 atatttaaaa agaatccgta tgggaaaatt ttggctgcct aaagatttga ataaagggca
     4801 atatcgaccc atgaccaaag aagagctaga acaagtgaga gaaaaataat tgaatggata
     4861 gctaaaagaa taagcagagt agttgtagcg ccatctgaaa agcagacttg tccgtctgac
     4921 tttcggatgg cttttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac
     4981 cgtttgttcg gaataaggga ctgtgacaag acttttgtta cagtccccat tgttccttgt
     5041 gactcagagt tagatagtaa ggacacgatc tccctaatac tcaggccaaa acgctgaatt
     5101 cacaacctct gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
     5161 aaaatatgag gaactatttt cccgtttttt tctctaatac tcaggccaaa acgctgattt
     5221 cacaacctct cctatacttt gtttcatttt ccctatactt tgtttattta ttctcattat
     5281 tgaaagcgct ataattacgt tgcgaatgaa tcgaaagggg tttttagatg aaaaaacgaa
     5341 aggggttcaa tcattttttt atgaatctat ggcgttataa agcacttgtc ctgatggctg
     5401 taccgggtat gctttggatg atttttttct tttacatacc tgtaatggcg aatgtcgtag
     5461 cattcaaaaa ttttcatatt tcagcagatg ggttcttggc tagcttgagg gaaagtcctt
     5521 gggtgggttg ggaaaatttc aaatttttat tttcttcgga tcaagcattt ctcatcacta
     5581 aaaatacaat tttatataat gtagcattta tccttttgaa tctgctgatc tcagtcgtat
     5641 ttgcaatcat tatgagcgag ttgagaaata aacgactcgt taaagtttat caaacgatgt
     5701 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
     5761 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
     5821 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
     5881 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
     5941 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
     6001 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
     6061 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
     6121 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
     6181 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
     6241 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
     6301 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
     6361 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
     6421 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
     6481 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
     6541 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
     6601 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttgat
     6661 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
     6721 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
     6781 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
     6841 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
     6901 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
     6961 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
     7021 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
     7081 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
     7141 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
     7201 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
     7261 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
     7321 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
     7381 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
     7441 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
     7501 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
     7561 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
     7621 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
     7681 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
     7741 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
     7801 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
     7861 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
     7921 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
     7981 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
     8041 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
     8101 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
     8161 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
     8221 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
     8281 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
     8341 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
     8401 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
     8461 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
     8521 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
     8581 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
     8641 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
     8701 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
     8761 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
     8821 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
     8881 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
     8941 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
     9001 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
     9061 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
     9121 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
     9181 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
     9241 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
     9301 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
     9361 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
     9421 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
     9481 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
     9541 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
     9601 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
     9661 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
     9721 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
     9781 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
     9841 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
     9901 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
     9961 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
    10021 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
    10081 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
    10141 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
    10201 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
    10261 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
    10321 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
    10381 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
    10441 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
    10501 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
    10561 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
    10621 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
    10681 aggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
    10741 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
    10801 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
    10861 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
    10921 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
    10981 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
    11041 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
    11101 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
    11161 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
    11221 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
    11281 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
    11341 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
    11401 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
    11461 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
    11521 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
    11581 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
    11641 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
    11701 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
    11761 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
    11821 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
    11881 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
    11941 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
    12001 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
    12061 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
    12121 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
    12181 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
    12241 acgcgtgctg caaaataaac aattagaagt cgctcaaaaa atgatcactg atttttttga
    12301 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
    12361 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
    12421 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
    12481 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
    12541 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
    12601 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
    12661 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
    12721 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
    12781 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa agaaaaagcc
    12841 aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt
    12901 acttgaggca aggcagcttt ttcatgacct ctcgataaac ggagttcaaa agccagtttc
    12961 tcggtattaa gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta
    13021 atactcgaaa ctaaacagct ttttcacgac ctctccacta aacggtgttc aatcagctga
    13081 ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac tattttgcca tttgtctctc
    13141 taatactcag gtcagaacgc tgatttcaca acctctcaat aaacggtgtt caaaagcttg
    13201 catctgcggt aataagacca accaaacgaa aaatatgagg atctattttt gcttggctgt
    13261 tcttattact tgatgcagga cagctttttc acaacctctt gttaattata gtttgataag
    13321 gaaaaaggca aatcttttgc ttgatatttc ttagggtgag ggatgatccc taattggtaa
    13381 gagatcgtgc cgcctttcag caaatcttga tgggtaaaat ataatttcgt atattctttg
    13441 tccatccgtt tgattttatg gataaattgt tgctgcggcc gattaggcgt tgcttcaatc
    13501 gtcaattcct ttccatttga caaaagcaaa acagccttgt caattaatgg cataccgata
    13561 acatattcac cggttccagg cgtgacagga taaaatccca gactattgaa gatataccaa
    13621 gcggccatac ttccattgtc ctcatctcca gggtaaccca ttggagaatc gtcgaaggtc
    13681 tgagtcatca actgtttaag cagtggctgt gccatttcag gttgtcctat ataactgaac
    13741 aagtaaggat agtgaaaact tggctggttt gaaatagcta attgtccaaa ttctatcgcg
    13801 gccatctcac tcatttcgtg gatctcaaaa ccatatcctc ctgggtcaaa cgttgggggc
    13861 tgattgcaca gctcaatgag tttttcttta tatctctcac tgccaccata ttttccgatc
    13921 agtcctgaaa aatcgtgata aacagcaaag ctgctttgcc aagcacttcc ttctgcataa
    13981 tcttgtcccc aacgattagc tgaaaatggt tctctgaact tgccatcttt atcttttgaa
    14041 cgcataaatc ctgactcata gtcaaagata ttttgatagt tcaatgattg ctgctgatat
    14101 ttcttcgtca tttcctcttc ttctagtacc ttggcaactt ggctaataca aaaatcgcta
    14161 taagcatagt ctaatgtgtg attgactgat tcgtggtagt gattaggaac atagccatat
    14221 gtcaagtaat cttctgttcc ttggcgtcca taatttggat gtttgctttg aaccgtagca
    14281 cctttttcca ttgccgttag aaattcaggc atcaagtctc gtcggatatc ttttacagca
    14341 gcatctgcaa tgaccgcatc gatcaatgtc ccaggcataa gtccacgttc atcaggagac
    14401 agccattttg gcaaatagcc actttctcta tagctgttca aaaaaccttc cagcatttct
    14461 tcgtattttt ctacagcaat caaagaataa agaggataaa ccgttttata cgtatcccaa
    14521 aacccattat ttgtatagag aacaccaggt tttatagatc gactagtcgt atcatagtgg
    14581 attttttgcc cactaggatc aatttcataa aatgtttgtg gaaataaaaa cacccgatat
    14641 aaattatgat aaaacgtccg caattgttgt tgatcatggt gtgtgagctg tatacgatca
    14701 aaataggact gccattgttc ttgactgtga gcaaggtaag tctctttgct ttggttttgt
    14761 tctagttcca ggttataagc tgcttgttcc gagctgataa aagatgtccc aaatcggata
    14821 gtttgttttt ttgtctgttc aaaatacaac gggatcgagt cgctattttt gttggaaaca
    14881 gctaactcat caattgttaa aggatattca aatctcaata taaaataaaa agaaaaatca
    14941 ggatcttctg aaccagcaaa atgtgtgatt tttccttcca ctgtgtaagg atctataaca
    15001 aagagctgat ggcgtcccgg aaaggataaa agtaaaccgc tgtcattttt gctgtaatca
    15061 atagataaga ttccgccata catgctaggt atcaatgtag aacagatgcc ataacgtaat
    15121 tgacagatcg acagatgagt cgggcaaaat atactttctt ctgggcggta agaactttga
    15181 gcatggaaaa gggtgttctc ctgtagtttt ccattgatcg gcgtaaacag catatgactg
    15241 aaatcaccca tccaaggact tggttgatgg gtaagacggt agccttgaaa aacgcggtca
    15301 tcaggatgaa accaccagct gccgttttga tccgtagttt gcggtgcaaa ataattcatc
    15361 ccaaaaggaa cgccagtata aggctggcag ttcccattag agaaatttgg ctgattcgct
    15421 gttgcatgtc tagtatctat ttgaccgata ttcattttat ttctcctttg ttgattgtct
    15481 tttgatgaat gaaactggaa atttactatc ggatggtcga tcttttgtct ctaaccaagt
    15541 gaccaaagca tctcccgcaa gcttacctaa ctgttcaaaa tcttgtgcga tggttgtcag
    15601 tggcgggtca acaagagctg ctgcttggat atcatcaaat ccgataattg aaaaatcttc
    15661 agggatatga taaccatgct gtctcaagag attcatggtc tggatagcga caacatcgtt
    15721 ttcacaaaca aaagcagtta cttcatactt agtgatataa gaaagcaatt catctaatgt
    15781 cgcgcttttg tcatccagtg aagtgtggaa agtcaaacca gctttgtcca atgcttcaag
    15841 gtaaccaagg taacgctgtc gaacggattg tggatgagtc tgcgtaccaa acaaataagc
    15901 gatttttgaa tgtccctgtt cgatcaaata agaggcagca agcgcaccgc cttggaaatt
    15961 atcggaaaga acggtaggaa aaggtaactc atagattttt ttatctaata tgacgacagg
    16021 gaattctttc aatgaaagct caaaaagtaa gtcaagatgt tgttctgtat caagagcata
    16081 ataaatcaat ccgtcaaatt cattgattag ctcttttgct gttttatttt gtagaaagtc
    16141 aagcgtcgtc atcatgacat caatctgttt atcttgcatc acagggttca atcccttgct
    16201 aaaatcacca acagacaaat cagcgacata tggaagaaga aacagaatcc gtcgtgtttt
    16261 tgtaggagaa gcaagaatct gttccggaga agcggattta acgaaactgc ctttcccgcg
    16321 aacgcgataa ataagtccta attgctctaa ttcagtcaat gcacgtttgg aagtgattct
    16381 gcttacttta taggtacgtg aaagttcttt ttcagttggt agttggctgt ctacaggtaa
    16441 ttcaccagtt aaaatagccg tttgcaaatc attaaatatt gtctggtaca atggttgatt
    16501 catataaaca cctcttaatg atatatcata ttcaaataat accataatgg atgaaaaagt
    16561 aaataaaaag atattttttg aaagcgtaga cataattggc tgtatgaagt ataatcttta
    16621 aagatatatc aaatagaaag aaggttttat tagtggctta taaagatata ccaaaatcgg
    16681 tgcagaaatt tatggaagat atcacacaaa aatgtgggga tatccatgcg gactgggcaa
    16741 agaattttac agcagcattt gccaatactc tgttgacaac agtcaaaaag caaaatgacg
    16801 gaaccacctt tttattgacg ggagacattc cagcgatgtg gttaagagac tctactgcac
    16861 aagttcgtcc ttatttagtg attgcaaaag aagatgagga attagctgac atgatcagcg
    16921 ggttagtcaa aaagcaattt ttttatatca atatcgatcc atatgcgaat gccttcaacg
    16981 aagaagctaa cggtgctggc caccaaacag atcatacaaa aatgaacgat tggatctggg
    17041 aaagaaaata tgaaattgat tctttatgct acccaataca gttggcctat cttctttaca
    17101 aaaatacagg aaaaactgat caattcaatc aatcttttat tgaaggtatc aaaaaaatct
    17161 tggacgtgtt caaaacagag caagatcatg aacactctcc ttatacattt gtacgagaca
    17221 cgacaagaaa agaagatacg ctgatcaatg acgggcgagg tgcaaaaaca gcttttacag
    17281 gaatgacatg gtctggtttc cgtcctagcg atgatacttg ccgttatgga tatctggtac
    17341 catccaatat gtttgcggtg gtcgtgttag gttatgtgga agaaattttt caaacgatac
    17401 tcgatgataa agaaattgta cagcaaacac agaaattgaa gaaagagatc caagagggga
    17461 ttcagaatca cggacgaacg gttaatcaaa aagggcaaac gatttatgca tatgaagtag
    17521 atggaatggg aaacgccaca gtcatggatg acagtaatgt gcccaatctg atcgccgcac
    17581 cttacttggg atattgttct gaacaagagg aagattatct gcaaacaaga agaacattgt
    17641 tgagtaaaga aaatccatat ttctatgaag gaaagtacgc aaaaggaatt ggatcctccc
    17701 atacgccaga aaaatacgtt tggccgattg cattagctat ggaaggaatg acaacgtcag
    17761 acaaatctga aaaagagcgg atattgaatt tacttgtagc aactgacgca ggaacacacc
    17821 ttatgcacga aggattcgat gtgaacaatc ctgaaaatta tacgagagaa tggttttctt
    17881 gggcaaatat gatgttttgc gaattagtca tggattactt tgatattaga gtagaaaagt
    17941 aggaggtatc gtaaatgaaa aagaaagtat atatcatttc tcatagccac tgggatcgtg
    18001 aatggtacat ggcttacgaa cagcatcaca tgcgactgat tgaattgata gacgatctac
    18061 tagagctatt tgaagttgat ccgtccttca acagttttca tttggatggg caaacgatta
    18121 ttttagatga ctatcttcaa gtaaggccag aaaaaagaca ggcaatccaa caagcaatca
    18181 atgaaggaaa attgagaatt ggaccatttt atatcttgca agatgatttt cttatcagct
    18241 cggaatcgaa cgtgagaaat atgctgattg gcatggaaga aagccgtaaa tggggaaccc
    18301 ctgtcatgtt aggctatttt cccgatacat ttggaaatat gggacagacg cctcaattaa
    18361 tgaaacaagc tggtatttca gcagcagcgt ttggccgtgg tgtaaaacca attggctttg
    18421 ataatcaagt tttggaagca gaaaactatt cttcccaata ctcagagatg tggtggaaag
    18481 gacctgatca aacagcaatc tttggtctgc tatttgccaa ttggtatagc aacgggaatg
    18541 agattccagt agaaaaagaa gcagctcttg ccttttggaa gcaaaaatta gcggatgcag
    18601 aacaatatgc ctctacgaac cacttattga tgatgaatgg tgttgatcat caaccggtac
    18661 aaaaagatat atcaaaagca atccatctcg caaatgaact gtttccagat tacgagttta
    18721 ttcattctaa ttttactgac tatctggaag cagttcaaaa agatgtgcca gaagacttag
    18781 gctcagtaga aggagaattg acaagtcaag aaacggatgg ctggtatact ttagccaata
    18841 cagcatctgc tcgggtttac ttaaaacaat ggaataccaa ggtccagcga cagctagaaa
    18901 atattacaga accacttgcg acgatggctt atgaagttag tggaaactat ccccatgatc
    18961 aattagatta tgcttggaag acattaatgc aaaatcatcc tcacgacagt atttgcggtt
    19021 gttcagtcga ttctgtccat cgggaaatga tacctcgttt tgaaaaagca gatgaagttg
    19081 gaaagtatct tgcacaagat tccttagaac agctgaccgc agcaatcgat acgaccggat
    19141 tcccaaaaga cagctttcca tttgtcattg taaatactgc aggaatggat aaaactggtg
    19201 aagcagaaat aaccatcgaa ttagaaagaa aaagattcgc tgaaggaata cctgaacaac
    19261 tatatcaaga gttagaaaat ttgcctaaac gtaagtatca tgtagaaaca aaaagtgggg
    19321 ccactatacc agctatttta tctgaagaaa ctgtccaatt tggttatgat ttgccgaaag
    19381 accgtttccg tgtgccatat atggcaagaa tgatcaaagt gactttacca ttagaaaaca
    19441 tgccagcatt ttcttgggaa acattcgcgc ttgtagaagg ggaagcgaaa gcagaagaaa
    19501 aggaaacaat gattcatcag agtggacgaa tcatcgaaaa cggccctcta catttgacga
    19561 ttgaaaaaaa tggtaccatt acaatggaag atcgcaaaaa caaacgaaag ttgaataatc
    19621 tgcatatttt tgaagatatc ggcgatatag gaaatgagta tatctttaaa caacctttct
    19681 gtgatcagcc aatcctatcc agtaacaaag aaaactctga agtaaaagta ttagtagata
    19741 caccggaaat agcaaaaata agtattctac aggaaatgga aatccctgtt tctgcagacg
    19801 agcgtttaga aaaagaacag cagatggttg tggaatttcg ttatcgaaaa gcagaacgat
    19861 ccaaagaaaa acgaatcctt caaataaaaa caatcatgac aatcagaaaa gatagtaaaa
    19921 aaatcgactt tgaaacgact ctcgacaatc agatgaaaga tcatcgctta cgtgtcttgt
    19981 ttcctacaaa gttacatgtg gaacatcacg aagcagatag tatttttgaa gtggtgaagc
    20041 gcccaaacca tgtgtctaaa agttgggaaa accccacgaa tccgcaacat cagcaagcat
    20101 ttgtcaatat tcatgatgaa gagtacggtg taacagtcgg aaactttggt ttgaatgaat
    20161 atgaagtaac agaagacgga caaatcgcag tgacgctact tcgcagtgtg ggagaactag
    20221 gcgattgggg atattttcca acaccagaag cacagtgtct aggtgagcat cggttcaatt
    20281 acagcattga actacatgga ccagaagaaa aattctctac ttatttgcat gcatacgcag
    20341 cacagattcc tttttccact caacaaatca aacatcatga aggaacattg atttcaaaac
    20401 aacaatattt aacgataaaa agtgaaacgt ttgcaattac agctttgaaa cgcagcaagt
    20461 ttagtgataa agtagtagta agaggtttca atatgtctag tcatttagaa aaattggaaa
    20521 taactaaaga caatggaaaa actgtcatat tgaatctgct tgaagaacca acgaagcaag
    20581 cggttgtccc aataatccaa ccttatgaga ttcggacaat cggattcgag gaggaaaacg
    20641 aatgtatggc ggaatagaag caggcggaac aaaatttgtg tgtgcggttt cagatggtgg
    20701 agaaatttta gaa
//
LOCUS       DANKAY010000047        20360 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000047 DANKAY010000000
VERSION     DANKAY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            291..1430
                     /locus_tag="PWY98_001649"
     CDS             291..1430
                     /locus_tag="PWY98_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2037403.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="PWY98_001650"
     CDS             complement(1497..2072)
                     /locus_tag="PWY98_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2037404.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="PWY98_001651"
     CDS             complement(2077..3051)
                     /locus_tag="PWY98_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL2037405.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="PWY98_001652"
     CDS             3334..4302
                     /locus_tag="PWY98_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2037406.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="PWY98_001653"
     CDS             4415..4693
                     /locus_tag="PWY98_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2037407.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="PWY98_001654"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="PWY98_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2037408.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="PWY98_001655"
     CDS             5150..5905
                     /locus_tag="PWY98_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2037409.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="PWY98_001656"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="PWY98_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2037410.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="PWY98_001657"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="PWY98_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2037411.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="PWY98_001658"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="PWY98_001658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2037412.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="PWY98_001659"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="PWY98_001659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2037413.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="PWY98_001660"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="PWY98_001660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2037414.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="PWY98_001661"
     CDS             10642..11184
                     /locus_tag="PWY98_001661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2037415.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="PWY98_001662"
     CDS             11207..12763
                     /locus_tag="PWY98_001662"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2037416.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="PWY98_001663"
     CDS             12777..13679
                     /locus_tag="PWY98_001663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2037417.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="PWY98_001664"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="PWY98_001664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2037418.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="PWY98_001665"
     CDS             15122..15544
                     /locus_tag="PWY98_001665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2037419.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="PWY98_001666"
     CDS             16013..16243
                     /locus_tag="PWY98_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2037420.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="PWY98_001667"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="PWY98_001667"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2037421.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="PWY98_001668"
     CDS             17654..17830
                     /locus_tag="PWY98_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2037422.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="PWY98_001669"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="PWY98_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2037423.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="PWY98_001670"
     CDS             18325..18708
                     /locus_tag="PWY98_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037424.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="PWY98_001671"
     CDS             18877..19761
                     /locus_tag="PWY98_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2037425.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="PWY98_001672"
     CDS             19875..20111
                     /locus_tag="PWY98_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2037426.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="PWY98_001673"
     tRNA            20199..20279
                     /locus_tag="PWY98_001673"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="PWY98_001674"
     tRNA            20286..20357
                     /locus_tag="PWY98_001674"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DANKAY010000048        19971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000048 DANKAY010000000
VERSION     DANKAY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            54..3443
                     /locus_tag="PWY98_001675"
     CDS             54..3443
                     /locus_tag="PWY98_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2037427.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3446..4867
                     /locus_tag="PWY98_001676"
     CDS             3446..4867
                     /locus_tag="PWY98_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2037428.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4864..6741
                     /locus_tag="PWY98_001677"
     CDS             4864..6741
                     /locus_tag="PWY98_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2037429.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6833..7675
                     /locus_tag="PWY98_001678"
     CDS             6833..7675
                     /locus_tag="PWY98_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2037430.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7827..8702)
                     /locus_tag="PWY98_001679"
     CDS             complement(7827..8702)
                     /locus_tag="PWY98_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2037431.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8909..9939
                     /locus_tag="PWY98_001680"
                     /pseudo
     CDS             8909..9939
                     /locus_tag="PWY98_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            10028..10333
                     /locus_tag="PWY98_001681"
     CDS             10028..10333
                     /locus_tag="PWY98_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2037432.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10512..12527
                     /locus_tag="PWY98_001682"
     CDS             10512..12527
                     /locus_tag="PWY98_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2037433.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12529..12813
                     /locus_tag="PWY98_001683"
     CDS             12529..12813
                     /locus_tag="PWY98_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037434.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12839..14212
                     /locus_tag="PWY98_001684"
     CDS             12839..14212
                     /locus_tag="PWY98_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2037435.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14295..16310)
                     /locus_tag="PWY98_001685"
     CDS             complement(14295..16310)
                     /locus_tag="PWY98_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2037436.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16332..17546)
                     /locus_tag="PWY98_001686"
     CDS             complement(16332..17546)
                     /locus_tag="PWY98_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2037437.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17766..18710
                     /locus_tag="PWY98_001687"
     CDS             17766..18710
                     /locus_tag="PWY98_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2037438.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19006..19950
                     /locus_tag="PWY98_001688"
     CDS             19006..19950
                     /locus_tag="PWY98_001688"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2037439.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6807 a         3680 c         3821 g         5663 t
ORIGIN      
        1 aataattttc actattcacg tcaaaacaga aaaaaagaaa aaggagggga aaaatgacga
       61 ccacagggaa gaaactgaaa gttattttca tgctgataat attgagttta tcaaactttg
      121 tgccattatc tgcaatagca gacactacag atgatccaac agttttagaa acaatttcag
      181 ctgaagtcat ttcggatcag tctggaaaaa aagcactgaa catcaagcta aatgcgaata
      241 acaccagtgc tgaaaagata gaaaaagaaa ttggtctagt cgaaaattac ttaagtgatg
      301 tggaaagaaa agaaggagat ggctatgctt atcaggtaaa tagcgggaaa attacgttgg
      361 aaatctcatc aaacactaaa caaactatcg atctgagttt tccaatcgat ccagcacttt
      421 accacagcca ggcaaacaag ctgatcgtcg ataataaaga atatgacatt attgatgaga
      481 cagaaaataa gaaagataca gatgtgtcag taccaaagcc agacgaaata gaagaagaat
      541 catcaaaaga aaacgaaaat tctgtcagcc catttacatt gcctacatta tccttgccag
      601 ctgtgagtgt gccatctaat caaacgattc ctacagaata tacaacagat gatcagggca
      661 cttatcctaa agccagttgg caacctacag gaaatacaaa tgttcttgat catcaaggca
      721 ataaaaacgg aacaaatcaa tgggatggta taaattcttg gaatggagat cctaatgatc
      781 ggacccattc gtatatcgaa tatggaggaa ccggtaatca agcagactat gcgatacgaa
      841 agtatgcaaa ggaaacaagt acacccggat tgtttgatgt ttatttgaat gctcgtggaa
      901 atgtacaaaa agatatcacg cctcttgatc tcgtattggt cgtagactgg tcaggaagta
      961 tgaacgacaa taatcggatc ggtgaagtaa agattggtgt cgatcgtttt gtcgatactt
     1021 tagcagatag cggtatcaca gacaaaatca atatgggata tgtcggctac tcaagcgaag
     1081 gatatagcta cagtaacggt gcagtacaga tgggttcatt tgattcagtg aaaaatcaag
     1141 taaaatccat tacaccttca cggacaaatg gtggtacttt tacacaaaaa gcactaagag
     1201 atgcaggaag catgctatcc gttccaaatg gacataaaaa agtgatcgtt ttgctgacgg
     1261 atggtgtacc aacattttcc tataaagtac agcgggtaca cgcacaatca agcagcaatt
     1321 attacggaac tcagttttct aatacgcaag atcggccggg aaatacttct ctaatctcaa
     1381 gaatctatga tgcacctgac caaaacaatc tatccagaag aatcgacagt acgtttatcg
     1441 caaccatcgg agaagcgatg gcactcaaag aacgaggaat cgaaatacat ggtcttggca
     1501 tccaacttca aagcgatccg gcagctggtc tctcaaaagc agaagtagag tctcgtatgc
     1561 gacaaatggt ttcatcagat gaaaaaggcg atctttacta tgaatcagct gatcatgcaa
     1621 cagatatctc tgaataccta gccaaaaaag ctgtacagat ctcagcaact gtaagcaatg
     1681 gacaaataaa tgatccaatc gcagaaccat tcatttatca gcctggtaca ctttcagtca
     1741 agagtgtggg gacaagtcct acaacggtca ctccatctat ttccatagaa ggaaatacca
     1801 tcaagagcaa tcagatctat ttaggaaaag accaagaaat ccaaatccat taccaagtga
     1861 gaatccaaac agaaaatgag gacttccatc caaatttctg gtatcaaatg aacggcagga
     1921 caactttcca gccaaacatt gataccaatg aattagctga attcggtata ccatctgcta
     1981 aagctcccgg agtcagtctt cacatcaaaa agttatggga agaatttgac aacaatctag
     2041 ctgatcgtcc agatcaagtt acttttgaga ttcaacggga acatacgaca aatgctgcag
     2101 cttggaaaaa cggatatatt cgaatcatta aaccagctaa agatacaaca aatacgtggg
     2161 aacgtgcaga cattgacaaa ttatctgcaa atagcggaga aagttatcaa gagatattat
     2221 cactacctca atacaataat caaggtcaag cattcagtta ccaaacaatc aaagaattac
     2281 ctgtaccagg atacgattct caacaaatag atgcaatgac atggaaaaat actaaacaat
     2341 tcacaccgtt aaacttgaaa ataacgaaaa attcctctac aggtgaaaag gatcttattg
     2401 gcgctgtttt caaattaaca ggagattcta ttgatacttt actaacagat catggcgacg
     2461 gaacctattc tcttccagaa aatgtcaaat tgcaaaaaga aatgacctat acgctgacag
     2521 aaacaaaagc tccagaaggg catggattaa gcaaaaagac tacttgggaa atcaagatcg
     2581 cttctgatgg tacggtaacc attgatggaa aaacagtcac tacttccgat gatacgatcc
     2641 agttgactat tgaaaatcct tttgttgaag ttcctgtagc agtacgtaag tatgcgatgc
     2701 aagggacgga caaagagata aatcttaaag gagcagcatt ttccctacag aaaaaagaag
     2761 caaatggtac ttatcagcca attgacagcc aaacaacgaa tgaaaaaggt cttgccagtt
     2821 ttgattcact cacacctggt aaatatcgag tcgttgaaac agctggtcct gccggatatg
     2881 atacttcgcc gggaaattat gaattccaaa tcgataaata tggaaaaatc atttacacgg
     2941 gaaaaaatac cgagatgaca aataatgtat ggacgctcac tcatcaaaat cgactaaaag
     3001 cgtttgatct aacggtacac aaaaaagaag acaacggaca gacattaaaa ggagcaaaat
     3061 tcagactgca gggaccagaa atggacttag aatcgccaaa agatggacaa gaaacagata
     3121 cctttctatt cgaaaattta aaacctggaa cttatacgct gaccgaaact tttacaccag
     3181 aaggatacca aggtctaaaa gagccagtta ctatagttat acacgaagat gggtcaattc
     3241 aagtggatgg acaagatcat gaatctgttc tgtcaccagg agccaaaaac aaccagattt
     3301 ctttagacat cacgaatcag gcaaaagtac cattacctga aacgggagga attggccgtt
     3361 taggaatcta tctagtaggg atgattggtt gtgcgttttc tatttggtat ctttttttga
     3421 aaaaagaaag agggggcagc taaatatgaa aaaacttggt tggcttagta tgtgtctctt
     3481 cttgttacta tttaaaccag cttttactca ggtagcaaca gaaacagaaa cagaaatggt
     3541 tcagattact ttacacaaat tgcttttccc aaacgggcaa ctgccgaaaa atcatccaaa
     3601 tgacggacaa gaaaaagctt tattacaaac gtatcgagga ttaaatggtg tcacattcca
     3661 agtttatgat gtcacagatt ctttttacca tctacgggaa aagggcaaaa cggtagaaga
     3721 agcacaagca gagatcgcaa aaaacggtgc gtcttccggt atgtttaccg cagaagcaac
     3781 aactacaact cttaacaacg aagatggtat cgcttctttt tctctggccg ctaaagatca
     3841 agaaaaaaga gataaagcgt atcttttcat tgaatccaaa gtaccagaag tcgtcaaaga
     3901 aaaggcagag aatatggtag ttgttcttcc tgtacatgga caaaacaatc aaaaactttc
     3961 aactatccat ttgtatccta aaaatgaaga aaacgactac cctgatccac cttttgagaa
     4021 ggtattagaa gagcctagaa atgattttac gattggtgaa aaaatcactt attccttgca
     4081 tacgacaatt cctgtaaata tccttgacta tcaaaagttc gaattgtcag atagtgcgga
     4141 tgaagcatta acgtttttac ctaatagttt aacgatttca tcgaatggag aaaagctgac
     4201 agaaggcttt gtcatacaca agaaacctca cggatttgat gttttatttt cgatcccttc
     4261 gttggaaaaa tatgctggaa aaaaactgac catttcttat cagatgcagc taagcagtac
     4321 agcacaggcg aacaaggaaa tcaacaacaa cggaacactg gattttggtt ttggtgtcag
     4381 tacaaagaaa gtctctgtat atacagggag taagcaattt gtcaaaatcg agacaaataa
     4441 accagataaa cgattagctg gcgcagtatt ccttattaaa aacaaagcag gaaattacct
     4501 ccagcaaaca gccaacggat acaagtggac aaagaacgaa tcagatgcgc ttcacctgat
     4561 ttccgataaa aatggcgctt tttcaatttc cgggttgaaa acaggaagtt atcgattaaa
     4621 agagatcgaa gcaccttctg gttatatttt aagtgaaaca gaaattccgt ttaccatttc
     4681 aacttttctt tctgaggata aagaggcgga cagtatattg aaagtagtca ataaaaaaga
     4741 aaatagccgt ccatttcttc caaaaacaaa cgaaacgaaa aatacacttt taggcgttgt
     4801 tggtatggta ttcgcaagct ttgcaatctg gttgtttatc aaaaaaagaa caggagtgaa
     4861 aaaatgaaaa atcataaaaa aataaacgtt atgttaggag tccttttcct tattttacca
     4921 ttactcacaa acagcttcgg cgcaaaaaaa gtgtttgcag aggagacagc agctcaagtc
     4981 atccttcata aaaagaaaat gactgattta cccgatcctt taatccaaaa cagcgggaaa
     5041 gaaatgagcg aattcgatca ataccaagga ttagccgata tttcattttc agtttataac
     5101 gtcactcaag aattttatgc gcaacgagat aaaggagcgt ccgtggatgc agcaaaacaa
     5161 gcagtccagt ctttgactcc tggtacacca gttgcttcag gaacgacaga tgctgatgga
     5221 aatgtcactt tatctttacc taaaaaacaa aatgggaaag atgcagtcta cacgatcaaa
     5281 gaagaaccaa aagacggagt gtcagctgcc gcaaacatgg ttttagcttt ccctgtatat
     5341 gagatgatca aacaagcaga tggctcttat aaatacggga cagaagaact agatactatc
     5401 catctctacc ctaaaaatac agtcggtaat gatggaacgt tgaaagttac aaaaatcggt
     5461 actgccgaaa acgaagcact aaatggagca gaatttatta tttctaaaga agaaggaaca
     5521 ccaagcgtca aaaaatacat ccaaagtgtc acagatggat tgtacacttg gacaactgat
     5581 caaaccaaag ccaaacattt cattactggt cattcttatg acatcggcaa caatgacttt
     5641 gccgaggcat ctattgaaaa aggccagttg atcgttaatc atttagaagt tggaaaatat
     5701 aatttagaag aagtaaaagc tcctgataat gcggaaatga ttgaaaagca aacaatcacg
     5761 ccttttgaga tcctggcaaa tagccaaaca ccagtagaaa agaccatcaa aaatgatacg
     5821 tctaaagttg ataaaacaac acctcaattg aatggaaaag atgtcgcaat cggtgaaaaa
     5881 attcaatatg agatttctgt caatatccca ttaggtatcg ctgataaaga aggaacgcaa
     5941 aacaagtaca caacattcaa acttatcgat actcatgacg ctgctttaac atttgataat
     6001 gattcttcag gaacgtatgc ttatgcctta tatgatggaa ataaagaaat cgacccagta
     6061 aattattctg tcactgagca aacagacgga ttcacggttt cagttgatcc gaattatatt
     6121 ccttcattaa ctcctggcgg tacattgaaa ttcgtttact atatgcattt gaacgaaaaa
     6181 gcagatccaa ccaaaggatt ttctaaccaa gcaaatgtcg ataacgggca tacaaatgat
     6241 caaacaccac cgtcagtcga tgtcgttact gggggcaaac gatttgttaa agtagatggt
     6301 gacgttacat cagaccaaac acttgctgga gcagaattcg tcgttcgtga tcaagatagt
     6361 gacacagcga aatatttatc gatcgaccca tccacaaaag ccgtcagctg ggtatcggcg
     6421 aaagaatcag caacggtttt tacaaccaca agtaacggtt taatcgatgt gacaggtcta
     6481 aaatatggca cgtactatct ggaagaaacg aaagcgccag aaaaatatgt tccattaaca
     6541 aaccgtgtag catttactat cgatgaacaa tcttatgtaa cagcaggaca gttgatttct
     6601 cctgaaaaaa taccaaataa acacaaaggt acacttcctt caacaggcgg taagggaatc
     6661 tatgtgtata tcggtgcagg agtagtcctt ctactgattg ctggactgta ctttgctaga
     6721 cgcaagcaca gtcagattta gtctttttgc aagaattgga aaaaggagca gcggattacg
     6781 caaatgctcc tttttctttt attatcaaac agtcatcaca aggagaacac aaatgaaacc
     6841 aaagaaaagg aaacttctag atctgctgat gcttttcttg cttctctcag gaatagggat
     6901 actagcttat ccatttgtta gcgacgcgct gaataattat ctggatcagc agatcatctc
     6961 tcattatcag caacaagcgg tcaaagaaaa tgaagaagtc atggctaaaa tccaagaaaa
     7021 catgacaaag aaaaaccagc aattagcaaa agaaggtgga aatccaggtg cagatccctt
     7081 tactaagaaa aaagaacctg cgaaaaagga tagaacctat tttgagaaac atacgatcgg
     7141 tattttgacg atccctgaga tcaatgtcca tttgccaatt tttgatgaaa cgaatactgt
     7201 gctgctagaa aaaggtgcct cattattgga aggaacgtct tatcctacgg ggggagaaaa
     7261 tacacatgcc gttatttcca gccatcgagg actgcctcaa gcaaagttgt tcacagactt
     7321 accgaaactg actttaggtg attctttcta tatcgaaatc aatggacgga cactcgctta
     7381 tcaagtcgat cagatcaaga cggtagaacc gactgataca aaagatctga gaatcgaaaa
     7441 agggcaggat ttcgtgacac tcctaacttg tacaccttat atggtcaaca gtcatcgctt
     7501 acttgtcaga gggcaccggg ttccttatca tgcaaaagaa gttcaaaaag aagtacagaa
     7561 agtatccgag aacaaacgat ttttcctgta tggattgtta gctgcatgta tactgatttt
     7621 cctccttctt atatatcttt ttcgtcaatc gttcaaaata agaaacagaa gttagctatt
     7681 cctcatttct ataatatatc tattcgaatc gttccgtaaa caaacaggct gaggtttgaa
     7741 aaagaaaact tcagcctgtt tttatgatga tatcaaagag agcctatcaa acactatctt
     7801 tcttattact aaatgaggat cttttcttaa gttttccgat attctttaag aaggcctttt
     7861 tgcatcaatt cttgtttgtt tttcaagttg cggatattct cttcaaaatt ttgagaatat
     7921 atcactgaga ataacgtatt taatacgtaa tcgaaggcaa tttgagagga gaaagtcgct
     7981 actttagcaa aatcatattc ctcctgcgat ataacaagac acatatcagc ctgtttggcc
     8041 atttctgaat gctgatttcc ggtaatcagc aaggtagggg cctctacatg cttcaaatag
     8101 gtcattattc gttcataatg atggatgcga ccgctgtagc taataaaaat ggcgcaatcc
     8161 tcgctagtca aattggctgc attccaagaa gaatcactgt attcttcagc catgatcaaa
     8221 aacttattca acttgaccat tttattctgg aatttccttg ccgtgatttg agaatcacct
     8281 tttgcaaaaa gaaagattcg ctgtgccttg ttcaatactt taccgatttg atctaaatct
     8341 tcatgttcaa tctgcaattg tgcttttttt attgactgga tcgataaatc cgccattttc
     8401 tttgcaattt gctgagtgct atcttcactt gaaaaaggaa aattaggatc aatcgtttga
     8461 ttgaactgag caatcaattg atgaacttcc cgccccagct cgaaacggaa ttctttaaat
     8521 ccgctgaatc ccattttttt gcataaacga atgatagctg aatgggaagt atacgtagct
     8581 tttgctaatt cttcaatcgt catgtagact gcagcttcga tattcctacg gatgtaatcg
     8641 acaatccgtt tctctgtcgt ggtaaacaac tcttgttttg ttagtttttc ttcaataagc
     8701 atgatctgac ctccaaattc tatcttactg ataaacactt ggttccgcta ctatcttttt
     8761 gatttttatc cttactttga acattaattt caccaaaccg aatgataaca agtgtttttc
     8821 tgaacttttt taccagaata gctattataa atactttctg cgcttttcta ggatatactg
     8881 aaaacatcaa taaaaggagg caccactcat gcttacaatc gcttatcttg gcaatggaaa
     8941 gagtacaaat cgctatcatt tgcctttttc aagaaaactt acgaacaaaa tcaatatcaa
     9001 aaaaattttt tccccatctg aaccagtgtg gaaaacattc gaagacatcg aatatactga
     9061 tcagctgtct gatctttggg atgatcctga gatccaatta gttgtcatca ctacacctag
     9121 tcaatttcat tatgaatatg gaaaaatggc actcgaacat ggtaagaatg tactagtaga
     9181 gaaacctttt gcagaaacgt caaaggaagc agaagagtta tttgcattag caaaagaaaa
     9241 aggtctattt atccaatgtt accaaaacag acggttcgat tctgacttct tgacagtcca
     9301 aaaagtaatc gaaagcggaa aattagggga tattttagaa gtagaaatgc attatgatta
     9361 tttcagaccg gaagtaccag aaaatacgca tgaattttct actgcgaaca gctatttata
     9421 tggacatgct tgtcatacag tagatcaagt tctttcttat ttcggcgaac cagatgatat
     9481 cagctatgat gtacgtcaac ttttagggaa aggacgaatg aatgattact tcgatctgga
     9541 ttttttctat ggaaatatga aaatttcgat aaaatccagt tatttccgca tcaaagaacg
     9601 tccaagtttc gtcgtttacg gaaaaaaggg atgttcgtga aacaaacaaa agatcgacaa
     9661 gaagaacatc tgaaaatgtt ttacatgcct gatcatgaag actttggtat cgatttgccc
     9721 gaacattatg gtgtattgac ctatatagat gaacaaggga gttatcatga agaaaaagta
     9781 atttccgagg ttggtgatta cagccgtgtc tacgaaggga tttacgagac attagttaat
     9841 ggcgcagaaa aagtcatcaa agatgaagaa acaatcttac aaatgaaaat tttggaagaa
     9901 ggaatcaaaa gcattcaaga atcagaagga aaaatatgac atatttgtaa gaatcgggtt
     9961 tgttttttga cgcataaact gtcttattgt acgatgaaaa agtatagaac tactaggagg
    10021 aaccaacatg acaaattatg acggtgaaaa acacaaacga ttaagaggtt caatggcccc
    10081 taaaacagaa gaagaagtgg ctttagcagg aactaattct ggtgaaacag aagacaaaga
    10141 agaatcatta gctggcacaa actctggtga aacagaaaat aaagaaacag ctttatctgg
    10201 ttcaaattct gtagaagagg aaacaaagcc taatgagccc aatcactttg aaaaagatat
    10261 cgaaaaagcg gttgaagatg tcaaagaaaa atttgaagaa ctgacctcaa aaaataaaca
    10321 tgatcatcat tgatcggacg aaatcaattc ataaaagcag gagacatttg gtcacctgct
    10381 ttttttctgt atccattcga tagaaatcgt tcggtacagt cgtaaaaaaa atccaactgt
    10441 aagttgtacc aaacacccct cgatttaaaa gcgctaccat actagaatat aagtaggagg
    10501 tgaacagcgc tgtgaactac aaaattagtt atctcctgaa aacaccaaga gaaatcagtc
    10561 tagaaaaagc agcagagatc atcgaagtaa atacaagcga ggcgatcaaa caattaactc
    10621 cttttgccga atatgtggat gatacccgta tccgtataaa gaaattccga gcaatcgact
    10681 gggcagaaaa atttttggat attactcgat ttgaagctgt gtataccgaa caagagcgaa
    10741 gagatcttct ttatttgatg gtttattcga ctgcaacgtc tgtttctgtt tttcattttc
    10801 aagattttct tcaggtttct aaagggacag tcctgtcaga tatcaaaaag ataagaaatg
    10861 aattagaagt ggaagattgt cagctgtcgt atactaggaa aactggtttt aggctgctag
    10921 ggtcagaagc aatattaaga agagaagcaa aaaatcgcac tgctcgtttg atgcagtcac
    10981 ttgctggaag atttgggctc acgtatttaa ccgcaaccct ttccattgat tattacgcca
    11041 aagttagaga tttgctagat aaaacgattt cactatcaga tcttcgattc gcaccaaatc
    11101 gaatggatga attagcttat tatttgagtt tgacaaccat tcgaattgga aaaacatcca
    11161 tggaactggt aaaagatgct gaactgttgg cttctcttgc agtcactgac attagtcgac
    11221 aagtattgac tggccttggc tattccgaac atcaaaattc ggagattata tttttctctc
    11281 tttgcttagc atcgatcgtt gaaggagatg tccaggagcc tgcacttgat tttttgctgg
    11341 aatgttcttc agaagtcatc catcgcatgg aattcctgat ggccgtagaa tttgcttcgt
    11401 ttcgtgaatt attgatgaat gtcttttatc acttggtccc tgcctatttt cgtatttcct
    11461 attcttttta tttacctaat gtgatgatcg atcaaattaa gcatcaatat gcttccatct
    11521 acgaaatgac aagaaaagca ttacttccgt tagaaagacg aatagggaag agcatccctg
    11581 aagaagagat tggttttttc actatactat ttggcggaga gatcagaaaa gcggacgcag
    11641 aagaaagaaa tagaaaaata agagccgtga tcgtctgtcc aagtggcatt agctcttcat
    11701 tgatcttaaa aagtgaactt cagcaattat tcccgatgat cttttttaca gagactaatt
    11761 cagctgaccg cttacacgag gttgacgaaa cttcttacga tattattttt tctactgtac
    11821 ctctcacact tactgataag aaaaagtctc tctatgttct gcatccaatc atgactgact
    11881 tggaaaaaaa tcagctgatc aatcaagtac aaagagattg gctgatacca ggattttcaa
    11941 tgccttctgc ccaagaattg ttgtcggcgc tactgcctta tatcgaatta aaagaaggcg
    12001 tagacgaaga aaagctctat caagtattaa atcgtaaaat gaacaaactt ttagaaaaga
    12061 aggaggatca acgtcctatg ctatctgaat tattaacaga agaaatgatc caattatcgg
    12121 atcaaccaaa aaactggcaa gaagcaatca catttgccgc acagccgcta ttatcagaaa
    12181 ataaaataga agaaagttat atcaaagcta tgatcgagcg cgtggagcag tatggtgcgt
    12241 ttatccatat tggcgatcac atcgcattac ctcatgctcg tccagaagat ggcgtaaacg
    12301 aagtagggat gtctttgctg aaacttcaac aacctgtgga tctgcttgat aatccagccc
    12361 atcccattag tttgttcatc tgtttagctg ctatcgataa tgaggcacat cttcgggctt
    12421 tggcaaattt aacaaagcta ctttctaata aacaaaatct tcaagattta ttacacgcat
    12481 caacaaaaga agaaatatta gcgatcataa gaaaaggaga cgaatgatat gaaaattttt
    12541 gcagtatgtc aaagtggatt aggaacaagt tttatggtag agatgaacat caaacaagca
    12601 cttgcagatc ttggagcaga tgtggacgag tttgaagtaa gtcatacgga tgttggaagt
    12661 gtatctagcg atatggctga ttatttcttc attgagcatt ctttgaaaga tacgctaact
    12721 tctatacctg atgaaaagct cgtacctttg caatcgatca ttgacagcga agaagtaaaa
    12781 gaaaaagtaa cgaagatctt gtcaaaagaa taaaaatgat tcaagggagg aactatcaat
    12841 ggataaattg ttgaatttgt ttgtgtcaat cgtcagtcag ccctccatcc tcgtttcttt
    12901 gattgcactg cttggtctcg ttcttcagaa gaagaaattt tcagaagtga tccaaggaac
    12961 tatcaaaaca tttgtcggat tcctagtact gatcggcggt gctggtttaa tcagtaattc
    13021 actaacccct tttgcttcga tgttccaaac agctctgcat actcaaggag tagttcctag
    13081 caatgaagca gtagtggcga ttgctttgca acaatacggt acacctactg ctttgatcat
    13141 gctagtaggg atgatcgtga atattttact tgctagattc acaagatata aattcatttt
    13201 tcttacaggt caagcaatgc tttatgtttc ttgtctaaca gcagtcattt tagtaagcgc
    13261 ggggtttact gcaaatttgt ggatgatctt attaggcggg gtcttcgaag gaacactatt
    13321 aactattacg cctgctcttt gtcaaccgtt tatgaagaac atcactggaa acaatcaagt
    13381 tgcgttaggg cataccggaa atatcggtta tgcttctagt ggatggatcg gaaagttttt
    13441 tggaaacaaa gaccgttcta cagaggacat aaagattcct aaaagtttca gctttttgcg
    13501 agattctact gtatcgatca tgctcttgat gtcgatcgta tatgtcgcac ttgctttgtt
    13561 agctggtcct ggatttgttg aaagagaact aagcaatgga gacaacgctg taatctttgc
    13621 actgatccaa gctggaacat tcacagctgg gtttgttatc gtccttcaag gggttcgaat
    13681 ggtgttggca gaaatcgttc cagcattcca agggattgct aaaaaactcg tacccgattc
    13741 taaaccagca ttagatgttc ctatcgtttt tccatatgca cctaatgctg tattaattgg
    13801 cttttttgtt agcttctttg taggaacact atccatgttt gctatgatag gcttgcaaac
    13861 tacagttatc attcctgggg tagtaggaca tttcttctgc ggagctgctt ctggtgtctt
    13921 cggcaactcg actggcggta gaagaggggc ggtattaggt tctgctttca atagcttatt
    13981 gattagttgg ctgccattat ttatccttcc agtattagga gatcttaaaa tcgcttcttc
    14041 tacatttgcc gatactgatt atctagtacc aggacttgta cttgggaaac taggagaata
    14101 cggtcaaata gccttgacag ttggaatcat cgctttcttt ttacttgtcg tattatctag
    14161 ctttcttttt gccggaaaaa gaacaaacat tgtttctaaa gaacagaatt aagcaaactt
    14221 caaaataaaa attgcttgga caatatcaga agaagattta aagtcttctg atatgtcgca
    14281 agcagttttt tgttttatgc atttattttt gaatagcttg ttaagacaag tggctgtttt
    14341 gctagaagag gataactttt accattcatt ttcactgttc cgcctgttct atcagaagta
    14401 atcacgatct tatcttttgt tttttcaagc gaaatctttc catctttgat aggtagcgta
    14461 caattcagtg tttcaaatga atctagatta ggttgtatca taaattcttc ataacctggc
    14521 tttgttggat acaaaccgat aaaatagcgt cctaaaagat agattgggct tgctccccat
    14581 gcatggcata agctttttcc ataaggatct ccatacatac tgtacttttc atttcctgtt
    14641 tgagtagggt cgtattcttc ccagaaagtt accgcatcat gagataacat ccctccccaa
    14701 taatcaagaa tcgtttggta gacgatattc gtctgtccga gtttgcataa agcatcttgt
    14761 tcatagaatt tgaaatatgg agtagtaatc tgtgtgatag tgtcattcaa taagacattc
    14821 tttaagatcg attgctgttg gtctttgtta gcaaaatcga ataaaacagc gaaaatattc
    14881 gcatgacgcg tcacatttct tctcccagat tcatagcaat cgataaaagc gccaagttct
    14941 tcatcccaaa agtagtcaaa taggttttgt ttcaatagtt gatatttttc ttgatagaga
    15001 tgagtatgat aattttcgtt ctctccatat gtttcagcta agatatctcc gcaagtgatc
    15061 atggaatgat aggctttcag taataagact tgttctgcag caagcgttcc ttctttatcc
    15121 atttcagacc aatcaataaa tatccaatct tttgctctcc cataaataaa ccctaattca
    15181 ttcgtttgct ccatcaagta tctcattaat gattgcatct tagggtagac gatgcgtaaa
    15241 aattcatagt ctttcgtcat catatagtgg ttttcgatac caatcaacca caagatcgaa
    15301 tagtcaacga tagtattcat atgctgcttg atttcatttt gtcctcttaa tgctaatatc
    15361 gttcgtttat tgatttcctc gtcaaaaaaa agatattgat tgatcatgaa tgattgatac
    15421 gcatctccag accaaatcca gcgatctcgt ttgataccgt cgataaaaaa caaatcacta
    15481 cataacttga aggcttcttc tgatactgac cagatctgat tcagtaaaga agaatcgctt
    15541 gaaaaagcag cttgtttcga aagagataaa aattcatgaa aaacttgaag ttgaaccatt
    15601 ccagtagaaa cctctggtat aaacaaatag cgaaaagcgc gtttacgagc tttcattttt
    15661 cttgtcattc ctatttgctt atagtagcaa taatcgatat ccatagcttc tacatcggac
    15721 tctccataac atagagtgat agattcgata tggtcatcca gagatattag ttctacacag
    15781 ccattcactg ctcgcccaaa atcgaataag attcctccgc caatctccgt ttcagtgact
    15841 ggatggacgc attctttttg ataatagatt tgatttggat cttttgcgat atcgacataa
    15901 agcggactcc agccagctgg atattcttca ataaaattac ttgctgtcca gccaatatct
    15961 gattttactt cgtctccctc gataaacatc gctggtagcc ctgatgtatg tcctacaaag
    16021 atacgaattt ttgctttacc agctggacaa gtcagccatt ttccacatgg atgtttttgt
    16081 ccattaattt ctacataccc aactccttgg atcgtcactt taaacatgga aggttgatca
    16141 agaaagtaat atctcttaaa cttgacgttc cgatggcaat catccatgta ccagtacgct
    16201 ggccagtcaa aacctcgttc ttcccgttgg aaattttgta gcagtccatg gcggatctca
    16261 aagtctccag gataccaaag ccatctatct ttatgttttt catttgtcat tttacacgac
    16321 ctccatcaaa tttattttgt tgtcaattgt tcttttattt cttcatctac tcttatcact
    16381 gtctttctat tcgtcctgtg tactactaga aggctaataa gcgcgctaat caacagcaag
    16441 atactgccat tgacgaaaac agcaagtgtt ttactcccaa gagaagcaaa agagagaaag
    16501 agcaaaccat atggtgcaat gaaagtacct aaattagcgc caaccaataa gattgaagta
    16561 ttgaaactct ccgcgtactt gggcgcttgg atacttactt ggttgaataa gtaaggaatg
    16621 aataacgaaa aagaaattcc tgttaggatc gcaccaaacc caatcacata caggtttatc
    16681 acagtggcaa tcaatacaaa gccgattccc atcagtagta aagaaagtgt aagcatatag
    16741 cgatgtgctt ttgtctgaag ccagccaaaa caaactccgg cagccatact tgccaattgc
    16801 atgattgtta agatattcgc tgcattagtt tcgtttccta gagaataagt cgtcaggact
    16861 gtggctaatt taatcgatac agcattatat accacgatga ccagcaagag aaaaatacca
    16921 taactaatca ttttaggatt cgttttgatt acttgtcttg ttggggctgt gccatgcgat
    16981 tgaagttcag ttgtttcagg tgtaggaaca aacaaaacaa acaagacaag aataggtaag
    17041 ctgatggcgt agactaaaaa ggtatattgc caaccgaact gtactagctg tcctgcaaca
    17101 aatgtcatta aagctgcacc aaatccttga aatgcacttt gaaaaccaat catagaggct
    17161 cgttcatctc cgtgataaaa ggcagcaatc atgctgactg ccaaagaatt gatcaatcca
    17221 aagccaatac ctagtccagc tcttgaagct aagacaatac tgtaactttg tgagaagagg
    17281 gggaggatac cacaaatcga tgcaatcact aatccgatga tcacagtttg tttttgtccg
    17341 attttcctag caataaaact actaatcaaa acaaaaatag tgaccataaa agagggagca
    17401 gtcgttaata attcaacagc agataaagaa cgcgtggcat aagtagctga catactggga
    17461 attgcagacg tgactgcacc agcagaagtc aaaataagag agactgacag cagagaaaat
    17521 ttcatcatct ttttattcat aagcatgttc ctttcatttt tgtaagcgct tatcaaaaat
    17581 atagtatgaa gtggttattc tctcaatagc ggaatcacag gaagttcatg tcccaaccat
    17641 agaaagtctt tatgaaacca aaaatagctt tgcatataat agcagtacag cgtgaactaa
    17701 taagcgaaag aaaccgctta caaaaaggag ggagtcaatt ggaactagct gaattagaca
    17761 tttttttgcg taacgaagaa tcaatcgaaa ctctgcagcg tgaacaagga atgaatatca
    17821 atgatttaaa cttagcttac catcttcaga caccgccaaa aatccaccga ggggatctat
    17881 ttcaacaggg aaatattcat atcagtaaac accgtcgata tgcggaagtt cccgagcata
    17941 cacatgattt tgttgaaatc aattatatgt atagcggtac atgtttacaa attattaatg
    18001 gagaaaggat agagatggaa gaaagtgacg tggtgatcat tgataaagaa gctactcaaa
    18061 gaatcgaata cacagggcaa gaggatattt taatcaacat cttattaaaa gaagaaacct
    18121 tatctacaga gatattgaat catttggcaa gcacaaataa tctactttct tcttttttga
    18181 taaatgcatc aaaagaaaaa ggggaacata cacaattcat tcattttcgc ggcggaaaaa
    18241 caagtcgttt gacgcatttg attgaaaata tgctgttgat ttattttgat acatacagtc
    18301 ataagatccg ttcgttaaat ctctatacat cactattact gatcgaattg actcatttat
    18361 tagaacaaca aagcttttca tcagcagatg gatgcgcaga aaatgagatg cttagagtat
    18421 tagattatat cgataagcat tttacttctt gtacgttaaa agaaacagca gctcattttg
    18481 gctacaatcc ggtctatctt agtaataaat tgaaaatgta ttttgatgct tcctttcaag
    18541 aactggttct aaagaaaaaa ttcgactttg cgttggaact gattcaagaa acgagttttt
    18601 cgttggaaga aatcgctaat cgtatcggat ataaacaaac gacttcttta ttcaaattat
    18661 tcaagaagta ttcaacatgt actcccaatg aatggagaaa taaacattaa gaagaaaatc
    18721 tttcactaaa gcatatgaag gaaaattgtt ttaggtgcga ttgctgtctt attatatcgt
    18781 tgcagcgaat ttttttggat ttattttcag ttctaaactt ttggttgtat tatcagggga
    18841 tataggtgta aacatttgtc atttatctgt tatgcgtatt caggaattcg tttccatcaa
    18901 aaattataat aacaactatt ataattaaca tgttgataaa atgaataaac aagactatac
    18961 ttttcatgaa agtaacaaat agttatgaaa aggagctgtt tattcatgaa aaaaacaagt
    19021 cgtaaagtag taatcgtcgg tacaggtttt gttggaacaa gtatcgctta tgcaatgatc
    19081 aaccaaggaa tctctaatga attagtattg atcgatgtga atcaagaaaa agcagaaggg
    19141 gaagcattgg accttttaga tggtatggct tggggcgatg aaaatgtggc tgtatggtca
    19201 ggtggctatg aagaatgtaa agatgctgat atcgttgtta tcacagctgg tatcaaccaa
    19261 aaacctggtc aatctcgttt agatttagtt aaaacaaatg cgtcaatcat gcgccaaatc
    19321 gtaaaagaaa tcatgggatc aggattcgac ggtattatcg ttgttgcttc aaacccagta
    19381 gatattttga catatatcgc ttggaacgaa tctggtctgc caacatcacg tgttatcggt
    19441 actggaacaa cattggatac aactcgtttc cgtaaagaaa tcgcattgaa attaaaagtt
    19501 gacccacgca gcgtccacgg ctatatctta ggtgaacatg gtgattcaga agttgctgca
    19561 tggtcacata caactgtcgg tggtaaacca gtgtttgaaa ttgtagaaaa agaccatcgt
    19621 attgcccaag atgaattaga tgtgattgct gataaagtcc gtaatgctgc ttatgagatc
    19681 attgatcgta aaaaagcaac ttactatggt attggtatga gtactgcacg tatcgtaaaa
    19741 gctatcttga ataacgaaca agctgtattg cctgtatctg cttatttaac tggtgaatac
    19801 gacgaaaaag atatctttac aggtgtgcca tcaatcgttg acgaaaatgg tgttcgtgaa
    19861 gttgttgaat tatcaatcaa cgaagaagaa aaagcaatgt tcagcaaatc aacaagtgct
    19921 ttaagagaag tattgaacac tgtattataa gaaacaatga atatgaacta a
//
LOCUS       DANKAY010000049        19970 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000049 DANKAY010000000
VERSION     DANKAY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(60..923)
                     /locus_tag="PWY98_001689"
     CDS             complement(60..923)
                     /locus_tag="PWY98_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2037440.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1119..3218)
                     /locus_tag="PWY98_001690"
     CDS             complement(1119..3218)
                     /locus_tag="PWY98_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2037441.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3438..4547)
                     /gene="rpoD"
                     /locus_tag="PWY98_001691"
     CDS             complement(3438..4547)
                     /gene="rpoD"
                     /locus_tag="PWY98_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2037442.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4571..6412)
                     /locus_tag="PWY98_001692"
     CDS             complement(4571..6412)
                     /locus_tag="PWY98_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2037443.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6594..8909)
                     /locus_tag="PWY98_001693"
     CDS             complement(6594..8909)
                     /locus_tag="PWY98_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2037444.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9094..9954)
                     /locus_tag="PWY98_001694"
     CDS             complement(9094..9954)
                     /locus_tag="PWY98_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2037445.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10013..10534)
                     /locus_tag="PWY98_001695"
     CDS             complement(10013..10534)
                     /locus_tag="PWY98_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2037446.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            10812..11573
                     /locus_tag="PWY98_001696"
     CDS             10812..11573
                     /locus_tag="PWY98_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2037447.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11599..14190
                     /gene="mprF"
                     /locus_tag="PWY98_001697"
     CDS             11599..14190
                     /gene="mprF"
                     /locus_tag="PWY98_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2037448.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14378..15565)
                     /gene="tuf"
                     /locus_tag="PWY98_001698"
     CDS             complement(14378..15565)
                     /gene="tuf"
                     /locus_tag="PWY98_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2037449.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15933..16289
                     /locus_tag="PWY98_001699"
     CDS             15933..16289
                     /locus_tag="PWY98_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2037450.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(16530..17534)
                     /locus_tag="PWY98_001700"
     CDS             complement(16530..17534)
                     /locus_tag="PWY98_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2037451.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            17700..18551
                     /locus_tag="PWY98_001701"
     CDS             17700..18551
                     /locus_tag="PWY98_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2037452.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            18568..18813
                     /locus_tag="PWY98_001702"
     CDS             18568..18813
                     /locus_tag="PWY98_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037453.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19000..19704
                     /locus_tag="PWY98_001703"
     CDS             19000..19704
                     /locus_tag="PWY98_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037454.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5650 a         4086 c         3508 g         6726 t
ORIGIN      
        1 ataacactat gttaattaaa attattataa gctagttaag aaaaacatgc aagggattct
       61 caattagatt ttttgactag ttttgtcaca ccgttttctt gtgtaatcaa tccttgtttc
      121 aaaagattac ctaatgcgcg cttaaatgct cctttgctga taccaaaagc tttcatgatt
      181 tcttctggat ccgatttatc tgtaaagttg atttgctgat ctgcagaacg ttctaaaatg
      241 gttaaaatca tttgtgcatc atctgatata gcttcatatg ctcttggctt caatgaaaga
      301 ttcagtacac cgtctggtcg gacaccaatc actcgacctt ttatctgctc gcctagacga
      361 ggttcattat atcgttcaga aggatggata aacccaagat agaaatcttc cgttaaaacg
      421 tacgtacctg ttaatttcaa gcggtatgct gttcctatca catctttgtt tttcatttct
      481 tctgttccat atttgcttaa agatttgaat attttttcat ctgctaaact gccccaaata
      541 cggtcttttg catcaactct aagggaaatc atcaagcgat cccctttttt aggccatagc
      601 tctgtcattg tcggcaattc atcaagagat acgacaaagt ctttatctgg aagaccgata
      661 tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga ttccaaaagc gtaatgaccg
      721 atacggcttt taggaatttg tgtagtgatc cgattttcat gtttttgatt ttggtaaccg
      781 aatccttcaa ctgcttcacc gattttgtgt tcaccttctg atttgcttaa gccaaaggtt
      841 tgtccgtttt tttggacaaa gtagtttttt tcattttcat caataataat acctgtaaat
      901 atagttgcta ataaatcgtt catttttttc tccctctata gtgaaaggtt ttcttaatac
      961 atttctttgc tatcataaca taatgttatc tacttgaata gctgagcctt ggtttggaaa
     1021 ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc ctttttcgaa taaacggtgt
     1081 taaacggcag gctcacagcc tctttcattt gcctaaaatt aattgctccc atttcctccg
     1141 aaccatccag ttttacagtt gatggttacg atgtaaaatg gcaaagcact ttctccaatg
     1201 cctttcatca atgcagattt tgttgaagtg gcatatagat tataatagcc ttccccattt
     1261 tcgattctgg ctttttctaa tctgtaacct ccttcaacga tatatccacg ttggatacat
     1321 gtagcaatca ctttgctttg gacacctggt gcccacgtcc atgctggatt agatgcatcg
     1381 gcaatatcac ttgctctggc agatttgatt ggctgattcg ctgagtttga agaggaaggg
     1441 gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg cagcttgttg agcggcttga
     1501 gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt cagctgcttt tttcgcttct
     1561 tctgcttttt tcttttcctc ttcttctttt ttctctttat cagtgagcat cttatctact
     1621 tgtttcaatt gttcattcaa ctgagcagat agatctgtat ttttcacttt ttctactgct
     1681 tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca ctacttttcc gtctttaaag
     1741 actatagctg ttttttcttt agctgtttgc aattcagcat attgattttt agcttctgtt
     1801 tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg tttcgattgc ttggatctct
     1861 ttatctgctt tcaacttgat gtcatctgct agatgatcat cgacgatggc aggtgctatg
     1921 aaccactcat tcactttttg gatatctcgg atcttagatt ggatatcttg gtaagttgtt
     1981 tctaactctg aatatccttt ttcattctta agactggata aatcattttt tagttttgat
     2041 aaatcttgat gaatcatatc tgcacgaata aattgatggt gatcatctgt ataaaaggca
     2101 gcaagtttct catgaagttc ttccatttgt tgctgtcttt ttgcttctgc tgctacttcg
     2161 gcttctactt tttgatgatg gtcataataa gcccagcttc ctccaccgag tagaagtact
     2221 gctgctaaaa cgagataagg tacttttttt gattttttct tttgcgcttt atctggatct
     2281 gttttttcta agtttgattc ttgatgtgtt ttttcagtat ctggcacgat tgttattgat
     2341 ttcgcttctg cttcaaacag cttaggctgg ttctcttctt tttctatttt tgcttcaaga
     2401 tcagtaccct ctttgcttac agattgattt tcagcatcgg attcactatt tatttcatcg
     2461 ggctctgttg actttttgtc tgtgttttcg agtatttctt ttttctctgc ttccggcagt
     2521 ttattcgttt tttccttttc taattgtttt tcagtaaata cttcagtttt ttctgtgata
     2581 tccgttttta cgtcttttat ttttactgtt tctttacctg tggcgttttc tctatcagag
     2641 tctttttcag tttcttgttc cttttctggc tctactttgt tttcctgtgc aattttttca
     2701 ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg tatcttcacg atgtgctttg
     2761 atataagcgg atagtatcga attttcttcc tcattatcga cgatttcttt cagttcagct
     2821 actcttcttt tttgttttga ttcaagcgtt tcttcttgat tctttcctga ttcttccgaa
     2881 gtctcatgaa cagttgcttt cttctgttca tttttctcta tattagttac ttgatcgtga
     2941 gcaggtggtt cttcgtcttc tggttctgct ttttcgaata attccattac agattctaga
     3001 ggtaaatctt tcaattccga ccattcgatt ggatcgttta attctgtatc aggaaagact
     3061 gcatcttcag ttgatttatc ctgcatagct gcatctgtat ttgtttcttc aattgattct
     3121 tttatttgct gatcttctgc agttatttga tgaccgcagt ctggacagat agtattctgc
     3181 tccatttttt tcgatccgca atttggacat tttttcatca cttagcgatc ggctccttta
     3241 ctaggtttac gctaccattt aatctatcat attctatacg tcacgccaat agcttatttt
     3301 taggatttac ttaaaaataa tgaatgaatt tccttcctat agaaaaaaaa gcttgagact
     3361 gcgtagtctc aagctttttt tcttttaaaa taaaccaaac agttttactg atttctcttc
     3421 tggtcaatag tccacgctta ttctaagaaa tcttttaatt gttttgaacg agaaggatgg
     3481 cgcaattttc ttaatgcttt agcctcgatc tgacgaatac gttcccgagt aacaccaaat
     3541 actttcccta cttcttccaa tgttctcgtc cggccatcat ctaaaccaaa acgtaagcga
     3601 aggacattct cttcccgatc agtcaatgta tctagtacat cttccaattg ttcttttagt
     3661 aactcataag ctgcgtgttc tgctggactt gttgcttcct ggtcttctat gaaatcacct
     3721 aagtgagagt catcttcttc accgattggc gtttcaaggg aaacaggttc ttgcgcgatt
     3781 ttcaagattt ctcggacttt ttctgttgga agatccattt ccgcaccgat ttcttctggc
     3841 gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga tacgtatcaa tttattaatt
     3901 gtttcaacca tatgaactgg aatacggatc gtacgtgctt ggtcagcgat tgctcgagtg
     3961 atggcttgac gaatccacca tgtagcatag gttgaaaact taaatccttt gcggtagtca
     4021 aatttttcaa ctgccttcat cagtcccatg tttccttctt ggatcaagtc aaggaactgc
     4081 atgcctcgtc ctacataacg tttagcgata cttactacta aacgaaggtt tgcttctgcc
     4141 agacgctgtt tcgcttcttg gtcgccttct tcgattttca atgctaacgc tacttcttct
     4201 tccgcagtta aaagggagac acggccaatt tctttcagat acatacgaac cgggtcattg
     4261 atttttacac cggttggcgc agaaagatct tctgtttttg cttgttcagc ttttttctca
     4321 gcactcttca agctgtgaat tgatggatca ccattttcat ccaccacact gatacctgcg
     4381 tcttcaatct tttggatcaa tttgtccatt tcatctgcat tcaaagtgaa tggtgtagca
     4441 agctgattgg ataaatcatc atatacaact tgtcgtttgg gtttgttttc tttgatgaaa
     4501 gctgctactg ctgcttcata cgttttttta tctgttccat ttgccataaa agaaggcccc
     4561 ccttgagtag ctatttggct tgttttaact gtttcgttaa attgatgatt tcaatcgtca
     4621 attcaagctc aagctgtttg tttccagttc gtctggcttc ttgctgcatt tgttttttta
     4681 atgttatttc atcagctaag ccagatttgc tgatcacatc tagcaagtct tggatttctc
     4741 gtggcgaact ttcttctgcc accggtaaat attcgatttc caccaccaac cgccttaaat
     4801 tttcatcttg caaaaagtct aaaaactctg caaggttgaa ttcataatgt tgtgagacat
     4861 atgcatcgaa taaaaaataa acttctgcat atgcatcatg aataaactga aattctcgtt
     4921 cttttaaaat attacggaca gaaacttctt taaatgcacg atataataga atctgctctg
     4981 ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt ttcttgtctc gtaacctgag
     5041 tgtgttgact ttcctgaggt tcttgacgat tttgctgatt tgtccttcga agatcgcgca
     5101 gttgtttttg tatcgtctct ctgccgatct gaaactctgt tgacagttga gatagataca
     5161 tatcttgttc gattggtgaa acaactcggc taagttcaac tagtaattct tcgaggtatt
     5221 cgatcttgtc tttttcatta tctaaatttt tcccttgttt taaataacgt gatttaaaag
     5281 taaagactgt ttcgcgccca tgagccacta attcttggaa agatgcttct ccatacttac
     5341 ggagatattc atcaggatca agcttttctg gaacgactac gacagaaagt gctaatcgac
     5401 tgttttgccg aagcaattca atccctcgat tggttgcttc aaaacctgcg ttgtctccgt
     5461 catagcaaaa gaccaattct ttcgtcagct tttccatttg ttggatctgt tgagctgtta
     5521 gacttgtccc catagaagct aatccagttt tgataccagc ctgccatgca gcaatcacat
     5581 ccataaaccc ttcgaacaac aaaatttctc cgtttttccg gatatcgctt cttgctttat
     5641 caagattaaa taaaacttgt cttttattga acagttctgt ttctggacta ttcaagtatt
     5701 tgggctgatc tttttcctgc cctttttcag gtgcaagcca gcgtcctgaa aaaccgatcg
     5761 tctggccttg agaattacga agtggaaaca tcactctttg ataaaaacga tcagacatac
     5821 ggccatcatc atgcgtgaca aacaatccag agcgttctaa caattcattt gaaattgatt
     5881 cattcttgaa tacttgctga agaaaagaac gctcattagg tgcaaaacct atgttgaatt
     5941 cttcaattaa ttctaaagac aacccacgac tttgcaaata ttctaaagct ggctgtccag
     6001 cttttgtatg caatagcatg tgatgataga tttccgctgc tttttcatga gcagcaatca
     6061 gtttccccgt agcagattca ggatttcgga cagctatagt acgctgacgc cattgatctt
     6121 ctaacggtat ctgttcgaag tctgccactt ttaccactgc ttctggaaaa cttagacctt
     6181 ctagttcttg aagaaaagta aacacgtttc cgcctttacc acaaccaaag caatgaaaaa
     6241 tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc ttcatgaaaa ggacacagcc
     6301 ccaagtaatt tttaccagac tttttcaact ggacatattg tccaatcacc tcaacgatat
     6361 tcgtttgact gcgtactgtt tcgataacct cttgaggaat ccgttgagcc attttttcac
     6421 cccctgctct attgatatct tttgggttta acagaagata tttcaggaga ataaccggta
     6481 aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac acaattatac atgtttgatt
     6541 gtatcatatt tcattgcttt ttcaagtata acacatttct atctcattaa gcatcaattt
     6601 gctactcttt tcagtaactg attttgaata tcagccttcc agtacaatac tcgaagaaca
     6661 atatttacga gcatgaatac aaagaagtat cctacgctct ctcccaaccc taagtttact
     6721 tccaaaattc catggaacag cattgcagct acatatatcc ctacgatcgt cgttactgct
     6781 aggaagatgc gcaatgggtt gaagggataa caagctttta caactgccat gcaactgatc
     6841 ccaatcagca agtaatacat caaagtagta gtttcagcat ttccccatgc gaatgttttt
     6901 ccaagtagat acaccgcaat gatattcact actacaagca aggcatttgg taaagcatta
     6961 ataagtgctg taggcaaata acggtaagtg atttttcttt tatcttgttc aaaagataaa
     7021 aagaaggaag gatacccttc aatcgctaaa tcaatcagcg tgatttggat agggataaac
     7081 ggaaatccaa ttgctgtaac ggcacatata atcgataaaa taaatgaata aatcgttttg
     7141 ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca ctcggcgtcc ttcaaacagg
     7201 acttctggca atgtagtaaa atcagagtcc aacaatacca gattcgcaat ctgacgtgtt
     7261 gctccatcac cttctgccat agcaatgctg caatctgctt ctcttaacgc caaaacatca
     7321 ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt ctttcaattc atttaccagc
     7381 aatttctttt gctgcggact cactcgtcca aaaaccgtat agcgatgagc agcttctcgc
     7441 acttccattt cttcagtaat ttgagaaaga tcgacatacg catcataatt cggtaatcct
     7501 gcacgtctag cgatattgga gacagtaaaa ggattgtctc cagaaatcac tttcagatcc
     7561 acaccttcct catgaagata agctagtgtt tcttgagcgt tttttcggat tggatcatcg
     7621 atttcaaaca gagcaaccgg ctcaacaaat gctggttttt cttcgttcaa ttgctgattt
     7681 ggagcgatcc ccaacataag tacgcgatag ccattcgctt gggctgtttt tagactgtcc
     7741 ggaagccgat cttcttccac caaacgttca ggcgctccta cgtacacgac tcccagttct
     7801 ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg cgactttgtt tgtcacatca
     7861 taccgattag aaagctcaaa ataatctctc atagcccgca tagtgatgtt gttatcactg
     7921 ctttctgcca gatacgtccc cataatttct gggatggctt tctcgtacag cggatgtagt
     7981 gcttctaccc gctggatact cattttccct tcagtaatgg tccctgtttt atccaagcat
     8041 aatgtatcga catgggccag cgtttcaatt gaatacatgt cttgaacaag gattcgtttt
     8101 tttgccagtt tggttacccc tgtcgttaat gcaatactta ttagcaatac caaacccttt
     8161 ggaagcatgc ctaacaaagc tgctgcagat gcaataacag aaattttgat agaagcatcg
     8221 cggatgaaca gtgcttccaa aaatagtatg atccccaaag gaataatcac gaaactagtg
     8281 aatttggata ctttgcgaat cgaatctaca agttcagaac gaatagcttt gtgagtcttt
     8341 gcctcgctag tcaagcggac agcataatta tctgcaccta ctcggataac ttcagccatg
     8401 acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag ctccagtttc tttgacaatc
     8461 aaatcagatt cgcctgtcaa aagagcttca tttgcttctg cccgtccaga tataacctgt
     8521 aaatcggctg gtatctgttc tcctgctccg atttttacca aatctcctaa taccagctct
     8581 ttagtagaaa tcgattgttc tttaccgtcc cggatcacta gtacttctga ttgggaaaca
     8641 atcgataatt tccgtacaag atttcttgca tgtatttctt gataaattcc aataacgata
     8701 ttgacgacaa taattgctaa atacgccatg ttggaatacg cacctacaaa taacagacaa
     8761 atcccaatac caaagttcag aaaattgaat aacgtcaata tattatcgct gaatatttgt
     8821 tttgtacttt ttgatgcatc ttcctggtag tcattttgct ggccagaagc cattttctga
     8881 actacctctt gctgtgtaag tccattcatt ttctttttct ccattttccg ccaactttca
     8941 aaaataatca cgtgtttaag tctagcgtgt tccaagatta aaaacaactt gtttttataa
     9001 aatttataga aaaacacacg aatgatttta caaaaaagat gccaaccttt ttgtttgaat
     9061 cacccgtgtg ctgtttttat tatcaatcta tctttactta tattgttcct ccaaacggct
     9121 catccaccaa cctagtgctg taccggctat aaaaagaaca ggacaagcta gtaccccagc
     9181 aactcccaat cccgcgtaat cggtaggaat gatcataatc gttgaagcaa aaactacacc
     9241 taaaatgaaa tggaatagct tgctatatgc ctttgagaaa atgtaatcca tgatttttga
     9301 caatccaaga acacaaagaa tcccaccaat tccgatcggc acaatcactt caagtcgtac
     9361 ttctttaaat ccgtcagcca tcgctttata caatcccata tacaccaaaa aattagaagg
     9421 gctcaatcct gggacgatca tacccaaacc aatcaatccg ccggcaatca gccatgttcc
     9481 aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg tataataaaa acaaataagc
     9541 cagaacaaaa gtaacaacca tgatgatcac atcagaagta tctcttcctt ttttccctgc
     9601 ttctttccat aacgcaggaa cagttccagc gatacatccg acaaaaaacc ataatatcgt
     9661 tgtttcatac gcacctaata aaaaactgac taaaaaagaa agtagaaaga tgcctgtcac
     9721 tccgcctaga ccaacaggca gaaaaaacaa aacattttct ttgaaatttt ttgtaaggtg
     9781 ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca gctgctaatg caccaccgct
     9841 gactcctggc aagataaatc ctgaaccgat aaacattccc ttcacaaacc gtaacatcca
     9901 gtctttggtt gtgtacccca cttcttgatt ttccattttt tgcctttttt ccactatttg
     9961 cgtactcctt ctttcattta attttattca tgattgaaca tttcctgcta ttttacttaa
    10021 agactttttc ataggtctct attatttttt cattcatttt gacaatatca ggataaaaca
    10081 tgttacctcc attatgatag agatagccgc cattgtaaag caaggactgg atccgccaat
    10141 atcggtattg tttcccttcc tcatttccta aaagtgggct cagcacatct ttagaatacg
    10201 cttctgctaa ttctactgtg ttttctccgc catgattttt tacataggca atataatctt
    10261 tcccaaaatt atatgcttgg acagctgtcc ataaatcgca tccttgtttc tttgcttcta
    10321 ctatcatttc cgccaaatat ttgacacctt gatcaatact gtcagctgga ttatcaatga
    10381 tctcagcttc tccatgcaga ctttccgagc tctgcatgag atctgtccct tttccttttg
    10441 attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt ttccatatga taattttttg
    10501 cagctgttgt tatttctatt tgatagcttt ccactcgttt taccgaacga taagttcttc
    10561 ctcataaaaa caacaccgca ccaataaccg tcagaatgat catgtaaaaa agaatacgga
    10621 taatagataa tcgttttcgc ttcatttcat cacctagtta ttttgacatc tttttttatc
    10681 ttactatttc tttatcttct tggctatttc ttccttgatt tctgtgtacg ccttaagaaa
    10741 aaatgaattt tcctgtattt tttgacatct tacgaaaatg atcgttgttt ttcagtaaaa
    10801 acaattgccc aatgactgat ataatcgtta tactttacac agtaggtgat tatgtgaaaa
    10861 aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat ttactatgaa aagagcggtc
    10921 aaggcttccc tctcttttta ctgcatggca atgacggcag cggccgcttt ttttcagaac
    10981 aggtccctgt actcgaacgc tactataccg tgtatctagt agatagccga ggacatggtc
    11041 gttcgacaaa cgaagcatct atgttgaatt ttcagttaat ggccgaggat ctgaatacga
    11101 tcatgttgct ggaaaaaatc gatcaggcag attttcttgg atttagtgat ggagcaaatc
    11161 tagcactggt atttgccagc agcttcccta aaaaagttca tcgattgatt ttaaattctg
    11221 gaaacacgct tgttaaaggc gttcgttttt ctgccagagt catcagtaat attcattatg
    11281 catgggtatg gttactctct ttatttcgcc caagtcttcg caaaaatctt ttagttatca
    11341 aacttttgct gcatgacatc gggcttacag aaaatgattt aaaaaaaatc aactccccca
    11401 ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa acattctttg tatatagcta
    11461 aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca aggacatgaa ttagctagaa
    11521 aagatcctga acgcttcaat cgagaagttc ttcaatttct ttctgaaaca taactattag
    11581 gaatagaagg tgatttattt gttaaaaaat accatacaat ggctgaaaaa ccacctagga
    11641 ctgttcaaaa caatcttttt gatttctgtc attgtcatta ttgtcggtga attgatgtcc
    11701 atcggtaaaa ctctatctat ccagcaatta ggagaaacgt ttgccactat acctgtatgg
    11761 aaaagcggac taatgctatt gattggtctt atctctgtgt tacctatggt tggttatgat
    11821 attattttga atcggatgct tgaacaaaag cagaatcctc gttacctgtt tgagacaagc
    11881 tggctgatca atacgatcaa caatattgcc ggtttcggcg ggtttgttag tgtggggctt
    11941 cgctctgaac tatatggaca aaagaaagag agcagaaacg tgatccaagc attatcgaaa
    12001 gttttcttgt ttttgatggc tggactttct atttacagtc tgctttcttt tttacttgta
    12061 gtcttcacgc cagttgctcc ttttttgaag caatattgga tatggttgat cggcggcggt
    12121 ctatattttc ctgtcgtctt tttatttact actttcaaaa agaaaggatt ccttggtggt
    12181 acctcactta aaactcaagg acagctattg ctagtttcat tgttggaatg gaccggtgtc
    12241 ctgtttagtt tcctttctgt tggttattta atggaaatcc ggattgatct gtggcaaaca
    12301 attccattat tcattgctgc ttcagtgata ggaatcgtgt ctatgattcc aggagaaatt
    12361 gggagtttcg atgtaatgat gattatcggg ttgtccgcta ttggtgtgcc aagagaaata
    12421 gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca tcattccgtt tttgattgga
    12481 atcgtctttt tcttcaaaaa tatcggttcc acttttgatc agcgttattc tggtatccct
    12541 aaacaattag caacagaaat tgcgcataaa atcgtggttg tcttattata cttttctgga
    12601 atcatgctag tactttctgc cactatcccg caagctttta cagagtttcg ctggcttcat
    12661 agtttgaacc cattgaaatt tcattttatt atccaatttc ctagtatact attgggattt
    12721 cttttaatca ttatgggcag agggatcgca gcacgtgtga aacgagcata tttgcctaca
    12781 atcttcttga ttgttctagc tttactttat gttttattaa gtgattttag tttcacaccg
    12841 gtggttttct tgtctattct gcttctgatc attcttgctt ctaaaaacga attatttcgg
    12901 gaacaattga tctattcatg ggagtggcgt acgatcgatg gaatcattat tggtgcactg
    12961 tccttgcttt acatcgttat tggggtatac aatctgccgg atttccctca tcggcgtcac
    13021 cattttattt cctttttctt atttccttct gaaaagattt ggttttcggg attgttggcc
    13081 atcatcgctg ttagtttcat gatcgttcta tttgttcatt tccttcaagg agaaaagaaa
    13141 cagattggcg aagcctttaa tgaagaaaag gccctgaaga tccttacaac ttatggagga
    13201 aactccgata gtcaattgat cttcttaaaa gataagcgga tgtttgccta tgaaaaagac
    13261 ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata ataaatgtat tgtaatgggt
    13321 gatccttctg gtaagaaaga agattttcca gaagcaatcg aagcttttat cgaagaaaca
    13381 gatcgactat gctatttacc agttttctac gaaacaagcg aagagattgt tatgattttg
    13441 catgaattcg gctacgattt cattaagatg ggggaagaag cttatgtaga cctgaatagt
    13501 tttacaactt cagggaaaaa aatgaagggc actcgtgctg tcttgaatcg gatcgaacga
    13561 gaaggattta catttgatgt cttacaacca ccattttcta ctgagcaaat gagtactttc
    13621 aaaaatatct ctgataattg gttgggttca cgaaaggaaa aagggttttc tttaggcttt
    13681 ttctctgaag attatctaca aagagcgcct attgcagttg tcaaaaatac ccaagaagaa
    13741 attgtcgctt ttgcaacgat catgcctact tacacgaaca ataaagtagg aacgattgat
    13801 ctgatgcgat atgattctaa aaaagcacct tctggcagta tggatttctt gttcttgcat
    13861 ttgtttgcct atatgaaaga agaacatatc caatggttca atttagggat ggcccctctt
    13921 tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa ggattgcata cttggtttac
    13981 gagtttggtt ctcattttta ttctttccat ggattaagag aatataaaag caagtacgct
    14041 acaaactggg tctcacgtta tactctatat tctagagata gttggattgc atatgttatg
    14101 attgctttgc tgattattga taatgcgcca gttgagcaga cacaaggtag aataagtggt
    14161 ttgaaaaaat ggatacgaag aaagtattaa aaactgcata ccgatagatg aacactctca
    14221 tgatgaataa aagagactga agtaaaacga aagttcatgc ttctacaatc tcttcttata
    14281 caattgaaga acaaagagcg gtccaaacag aaatcctgtt tggaccgctc ttttatttta
    14341 aacatttttc attaccaaaa tattcgagtc gcgtttctta tgcttcgatc tctgtaacga
    14401 tacctgaacc tactgtccgt ccgccttcac gaattgagaa tgtcgttccg ttttcaacag
    14461 cgattggatg gatcaagtca acatcaatcg tcacgttgtc accaggcatg accatttctg
    14521 tatcttctgg caatgtgatt gttcctgtaa catcagttgt gcggaagtag aactgtggac
    14581 gatagttgtt gaagaatgga gtatgacgtc caccttcttc ttttgtcaac acgtacactt
    14641 ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt agcaagtact tgtccacgtt
    14701 cgatatcgtc acgttggatc ccccgtaaca acacgcctac gttatcccca gcttccccgt
    14761 aatctaacgt tttacggaac atttctacac ctgttacaac tgctttttgt gtttcaggtt
    14821 tgatccctac gatctctacc tcatcaccga cacgaacagc accacgatca atacgacctg
    14881 aggcaacagt tcctcgacct gtaattgaga agacgtcttc cactggcaat agtaatggtt
    14941 tatctgtatc acgttctggt gttgggatat attcatctac tgtatccatc aattccataa
    15001 tagcagcttc agcatctgga tcgccttgca atgcttttaa tgcggaacct ttgatcacag
    15061 gagtgtcatc gcctgggaaa ccatattcgc tcaataactc gcgaacttcc atttctacca
    15121 aatcgatcaa ttcttcatca tcgactaaat caactttgtt caagaaaaca atcaggtatt
    15181 ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt ttgaggcatc ggaccgtcag
    15241 ttgcagaaac aactaagatt gcaccatcca tttgtgctgc ccctgtaatc atgtttttca
    15301 catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg gcgtttttct gtttcatatt
    15361 ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc ttctggagct gcatcaatac
    15421 tagcatagtc ttgaggattt gctaggcctt ttttgcctaa tactgtagtg atcgcagcag
    15481 ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc aatgttaacg tgtggtttac
    15541 ttctgtcata atgttctttt gccataattt acaaacctcc taaataatat agatcactga
    15601 aaaatttcag ctctcacctt tttattatag tctttttttg actaaatcaa aaagaatacg
    15661 tccgacttac tttttgtaag attcgaacta agcttattat acgtcaatat tggtcaaagt
    15721 caactaagag cactcgtctg tcttatacgc gaaaaaagta ccttttataa ataaaaaaat
    15781 gtgttttttg agaaaaactt caagatagtc cttaactttc ttttgaagca ataagaacaa
    15841 aatgaggaaa agtttaaaat tcagctgttg ccaacaactt tcaatagacg aaatcttcta
    15901 tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa attaattggc gtacttgtcg
    15961 ttttaggcat atttctcggc ggaagttttg cagcttatca ttatttttac ggcggcgaag
    16021 cttattatac aaaaatcacg acgaatggcg aaatgtcagc tacccaagca gataacggag
    16081 agaaattcac gacttatacc tacaagcaag cggcttataa taaagatggt gaaaaaaaac
    16141 aggtgacgct tcgcgaagaa agagaacatc cattaaagat gaatgcgtat cttaaactca
    16201 aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat aaaggcttct gaagtgccta
    16261 aaaatgcggc agaaaaaatc gataattagt aaatggtctt aaaattttat tagataaaaa
    16321 gaggctggga cagaagcgtt taactccaag aaataagaag gagttgcttc tgcttccgcc
    16381 gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtctc acgctctttt
    16441 tttgttatat tcgtcttttc cgatccttct cttgacacaa ttcatttaac tgatcactgg
    16501 tacttgaaca cctaatattc tagtcatttt tataaacgat tcaatacttc atccacattt
    16561 ttgatcatca cggatatcgt accatccgga ttattcacaa attcaatcaa atctgggttg
    16621 cgatatacat ctactggtac gatcagttcg atcccgtttg atagcttgaa tttttgtttt
    16681 ccaaattttt tctcagaaat ttcttgtact tcttttacag gaggtgcttc tgggacaaat
    16741 ccttgctctt gtacttcttc tttaaaagcc atttttgccg tgatgttttc cttgaagact
    16801 ttttccgcaa tcatctcatg atccaaccga cctttttctt ctatcgtatc atagacagct
    16861 tctttcaaat cagccatgat atcaaattct tctgtctcaa atttttttcc aagatgcttg
    16921 gctacttttt ggatcacttt tacattttct tctaaagaag gtgcaggaac actttcgatc
    16981 actcttccag aaaaataaag ctcttttttg ccagaaaact catatttttt ttcaattaat
    17041 tcgtaggtcc aatcagtcaa attcacagcg aatgcttcat ctgctttttg tgttttcgcc
    17101 cctagaattg ctcgattcaa aatcaagcgg ttgtcgatcc ctgcttcgtc tgcttcaaca
    17161 aagtgagtgt aagcctcacg ataattgact ttcaataaag ataagtacat cactgtatct
    17221 aattcataca agacaacaaa tacatctgca ctaggtgctt cttcactttc ttggtagata
    17281 tcgaaccacc gctgaacgat tgcttcactt gcttccacaa aattttcttg agcgatttta
    17341 agtgattcaa cgatcatgct ttcttctgct agttgtccag tttttgtttg agcagaagag
    17401 attttttgga tcttcttttg aagaaaatca cgaatatatt cttttgtcag atccagctct
    17461 ttttgtgaaa agactggaga accagtttct ctgtcaataa tatgcagaat tgcttttttt
    17521 aaatagatat ccattttctc attcccttct gtcttagttt acggaaaaaa aatcaaaaat
    17581 accagaatca aaatcatttt cttttaaaca aaaatttcaa gagtagaaac aaaatggatt
    17641 ctttttttcg aaaaatgcgc tacacttata gttgaacgaa taaaaaagga gctgaaagta
    17701 tgaattcatt aacaagcact tatcgtctat caaatggtta cgaaatcccg gtagtaggat
    17761 tcggcacatg gcaaacacct gatggggatg tcgctgtttc ctctgttaaa gaagcgctgg
    17821 ctgcaggtta tcgccatatc gatactgccc aaggatacaa gaatgaagaa agcgtcggcc
    17881 aagcgattaa ggaaagtgga attcctagag aagagatatt cttgacaaca aaattatgga
    17941 atgcaaacca cagctatgaa cttgtgatgt cttcttttga agaatcattg aagaagctcc
    18001 aaacagatta tttagatctg tttttgatcc actggccaaa ccctgttgct tttcgtgata
    18061 attgggaaca agcaaatgca gatacatggc gagcatttga agaactgtat gaagcaggta
    18121 aaatcaaagc aatcggtgtg agcaatttct taccccacca tctagataca ctagcaaaaa
    18181 cagcaaaaat tatgccgatg gtcaatcaag ttttccttgc accaggcgaa ttacagacaa
    18241 ctgtagttga gtatgcaaaa aaacatgata tgatcctcga agcatatagt ccattaggca
    18301 ctggtaaaat cttcgatgtg cctgaaatga aacaaatcgc tgaagcacat gacaagacga
    18361 ttgcacaagt tgctttgcgt tggtcgctgc aacacgaatt ccttcctctt cctaagtcag
    18421 tgacaccaag cagaatcaaa gaaaatactg aattatttga ttttgaatta acagaagaag
    18481 aaatgaaaca aatcgatcag ttagatggtg tagtcggcaa agcaaaagac ccagatacta
    18541 cccaatttta agtcgaaagg tgattttatg gatccaatcg aacgcttgaa ttctctctcg
    18601 gaagaagtta cccagacttt tcattccgat tttgtctttt tgatcgatgc agagaaaatc
    18661 caacattttc ctgcaagaaa ctggacacat gatcaaatca tcgaagaact gaaaaaacgg
    18721 ttcgatcatt cgctaatggt caaaccctgg cacgaacatg aagtcattta ttcacctgaa
    18781 ctacctgttt ttgccttgat accgaaaaaa tagaaagaat ggaacggatt atgtatatat
    18841 acataatccg ttccattttt tatttttctt tcaaaaaacg ggaaaatacg caaatttttc
    18901 tgttgtaatt tgcgtatacg caagtttttt tgcattactc cttttcattt ggctgttttt
    18961 ttcttataat cgaaagggta tgagcgaagg agagaacata tgaagaaaaa acagaaacaa
    19021 aaattattaa atcaattccg tccttcattg gatggtgtaa gaggacaatt attccgttct
    19081 ctagaagaag agagcttgcg acgttatggt ttgccgttac aagtcatgct tgatcccaaa
    19141 aaccgtcaaa tcttaacgat cggattagct gggaaaacaa atgaagatac acggatcaac
    19201 gtgccaatcg atgacaactt caatactgta ttaaagagaa ttcgttcagg agaaaacggg
    19261 attttcgaac gattccgtga caatcttctt attgaaatcg tgtcttactg gaatgatcaa
    19321 cgattgaaaa acgatgaacc aactgcgcaa gctgttgcga caccagttac agaagaaaca
    19381 gtcagtgtgc cagaagaaaa aacggaaaaa ccaaaagcgg acacaactga aaatacacgc
    19441 tcaactgata cgaatgccgc tttaagtttt gctgattttt caaaagccgt tgctgagtat
    19501 cctaaatttt acacagaaca aaatgaacat gaagtagtta ttaatgaaaa aaatgctgat
    19561 gaacctcgta aattagcaac tatttctatg actactgaaa atgagttcgt tatcgaaaaa
    19621 gcactcgaac ggaaatacaa agtcaaattg actttgattc ctttgatcga acaatttgca
    19681 gcgacagcga ttactgatcg ataaaaattc tatttgtcat aaaaggctgg gatttaaaat
    19741 cccagccttt ttagtcatat aataatttct acagcaagta aaaagaaatt ccttctaaca
    19801 tcaggctcaa atatcagctt gacctgtcca acaaatcacc tatgatccta caatgttagg
    19861 cgacttctct tctacctgaa gatatcttgg agttgcctga tttcttaaga tgcctaacct
    19921 taactataaa aaattctaat aataaacata ttttctgata gagaaaaaag
//
LOCUS       DANKAY010000050        19832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000050 DANKAY010000000
VERSION     DANKAY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            5..193
                     /locus_tag="PWY98_001704"
     CDS             5..193
                     /locus_tag="PWY98_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037455.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
     gene            complement(837..1790)
                     /locus_tag="PWY98_001705"
     CDS             complement(837..1790)
                     /locus_tag="PWY98_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2037456.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1813..2568)
                     /locus_tag="PWY98_001706"
     CDS             complement(1813..2568)
                     /locus_tag="PWY98_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2037457.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2565..3524)
                     /locus_tag="PWY98_001707"
     CDS             complement(2565..3524)
                     /locus_tag="PWY98_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2037458.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3517..4467)
                     /locus_tag="PWY98_001708"
     CDS             complement(3517..4467)
                     /locus_tag="PWY98_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2037459.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4617..5069
                     /locus_tag="PWY98_001709"
     CDS             4617..5069
                     /locus_tag="PWY98_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2037460.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5158..7425)
                     /gene="gshAB"
                     /locus_tag="PWY98_001710"
     CDS             complement(5158..7425)
                     /gene="gshAB"
                     /locus_tag="PWY98_001710"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2037461.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7612..8610)
                     /gene="ftsY"
                     /locus_tag="PWY98_001711"
     CDS             complement(7612..8610)
                     /gene="ftsY"
                     /locus_tag="PWY98_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2037462.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8621..9430)
                     /locus_tag="PWY98_001712"
     CDS             complement(8621..9430)
                     /locus_tag="PWY98_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2037463.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9439..13020)
                     /gene="smc"
                     /locus_tag="PWY98_001713"
     CDS             complement(9439..13020)
                     /gene="smc"
                     /locus_tag="PWY98_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2037464.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13040..13726)
                     /locus_tag="PWY98_001714"
     CDS             complement(13040..13726)
                     /locus_tag="PWY98_001714"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2037465.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14074..15855)
                     /locus_tag="PWY98_001715"
     CDS             complement(14074..15855)
                     /locus_tag="PWY98_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2037466.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15881..16795)
                     /locus_tag="PWY98_001716"
     CDS             complement(15881..16795)
                     /locus_tag="PWY98_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037467.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16822..17784)
                     /locus_tag="PWY98_001717"
     CDS             complement(16822..17784)
                     /locus_tag="PWY98_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037468.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17784..18728)
                     /locus_tag="PWY98_001718"
     CDS             complement(17784..18728)
                     /locus_tag="PWY98_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037469.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18729..19733)
                     /locus_tag="PWY98_001719"
     CDS             complement(18729..19733)
                     /locus_tag="PWY98_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037470.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5492 a         4264 c         3528 g         6548 t
ORIGIN      
        1 agcattggat atttataaaa aattagcttc aaaaaaaacg aatagttttg gattgatatt
       61 aagcgagtat aaaatcaaag cagaagagag ttatttctat caattactaa aacggtggag
      121 taagcttccc gccgattttt atcgaaaaat gcagatcatc aactatcaac cgcttgctga
      181 gacacatgcc taaatagagg ttgtgaaatc agtgctttga gctaataccg aaggtcagct
      241 gattgaacat tgtttagaag agattgtgac aactgcgatt tgctttgagt aataagaacg
      301 ctggacagaa aatagcttct catattttct agtctagtgg gcttattgcc gagaagcagc
      361 agatggaaca tcgtttagaa gagattgtga caactgcgat ttgttttgag taataagaac
      421 gctggacaga aaatagcttc tcatattttc tagtctagtg gacttattgc cgagaagcag
      481 cagatggaac atcgtttaaa gaagagatcg gtacaaaata aaaatatcta aatttaagtt
      541 ttatctaagt ttcgctttgt acctttgatt caaatcaaag gaggcgatct tgatgctgaa
      601 aaaatggatc acactatttt ttgccactcc ccattctaga atcaacccgt atgatgtaga
      661 taatgaattc acgttgaaaa aagatttctc aaataaataa aaaccatccg taaaatcatc
      721 aatacgattt tacggatggt ttttgataac ttctttgagg ataggctctt gctatttaag
      781 aatgtaagaa acattattgt tacatagtat gagagtttaa tcctctgtta tttatgctat
      841 ttaaatgctt gattcacatc ttcgatcatc agtttcattg attctaaacc gcccccgctt
      901 aaataccaga catctggttc aagggagatg atctgctgat ttttaccagc attcgtttgt
      961 gctaccagtt cattggcaga aatatcatct ttgctgtcgt ctccaccaat agcttttgtt
     1021 ctgtctacta caaataagat atccggattt ttttccagca catattcata agaaacactt
     1081 tgaccgtgag tggatgcttc gatttgatca tctgcttgtt caaagccaaa taaatcatgg
     1141 atgaaaccga atctagaacc agatccgtag gctgataatt gcccttcatt cacaagtgtg
     1201 actagtgctt ttttgtcagt agcttctgct ttctttttcg tctgatcgat tgcactagat
     1261 aattcctcta attgagtttt agcttcttct tgcttaccaa agatctcccc taaagcagtg
     1321 acattttgct ggaaagattc ccatggattt ttcgcatcta aagaaaggaa aatggtcgga
     1381 gcaatttgac ttagatcttt ttgaaaatct ctttgtcggc cagaaatgat gattagatct
     1441 ggctgaagct gattaatttt ttctaagtct ggttctttta ttccacctgc tgattctact
     1501 tttttatagg atgaaagata ctcaggaagg ctgcttgtag ctgctccaac taccttatct
     1561 ccaacaccaa gagcatccat cgtatctaaa gaaccattgt cgaatacgac gactttttct
     1621 ggattatttg gcacctcgat ttggtctcca ttactatctg ttatcgtcaa tgtagtttca
     1681 gtttgtgtgc tgcttttttt tgtgtctgcc tgactggtgt tgttgctatt tccacatgct
     1741 gctaaaccaa atgatgctag aatcaagata cctactacta attttttcat ctcttctctc
     1801 ctaatgttag ttttagaaat aaagacagaa tctgcggcct tcaatctcac aaatccgtat
     1861 gtccatttcg tataattcat ccaatacatc tttttttatc atttcttggg tcgttccttg
     1921 cgtaaataac tgtccatctt tcattgcaac aatttcgtct gcataacttg cagcaaagtt
     1981 gatatcatga agaacaataa caattgtttt cccgatttct tgtactaaat tttttaaaat
     2041 ctgcatgatt tgaaccgcaa aattcatatc tagattgttc aaaggttcat ccagtaaaat
     2101 ataatccgtg tcttgcgcta gcaccattgc aatatacgca cgttggagtt gaccgcctga
     2161 aagagtgtca atcgattgat ccgctatttc ttctaaccca atgtgttcca gtgattcctg
     2221 aacgatttcc tcgtcttttg tcgtcaattt tcctttcgta taaggaaatc gtccaaattc
     2281 tactaattct ttcacggtga tttggacatt tatttcattc gattgtcgta ggatcgataa
     2341 ttttttggct aattcattgg atttccaagc ctttacttcg ttatggtcta aatagatttc
     2401 acctgtatct tttggtatca aacgactgat catcgacaac accgttgact ttccagcacc
     2461 atttggaccg ataaaagcag tcaacttctt ttcaggaatc gttaactgta catctttaac
     2521 cactgatttt ccatggtaca gtttattgac cttttgtaaa tcaatcatag gatactcttc
     2581 ctttcgcgaa tcaattttgt gacaaaataa attcctccga caaattcaac gacgacactc
     2641 actgttgtat tccagccgaa aatctgctct actgcgagct gtcctcctgt cagaaacagc
     2701 atagctacca gaaagcttcc gataaatagc tgaccatgac gataagtaga aaagacacga
     2761 taactgatat ttgcgacaat aaaccctaaa aaagtaactg gacccacaag agccgttgat
     2821 gtccctgtca aaagactgac acaacataaa atcaaaaatt caaaacgatc tgtgtctatc
     2881 ccgaggttta ctgcttgttc atttccgaga tggaaaacgt ctagcacatg tcgatatcga
     2941 aataacaggt accagcagcc gataatgatc aaagaagcag gcaaaagata ctgactctgg
     3001 atattgccaa agcttgcaaa taatctgcct tgaagcaaat catattcatt cggatccatg
     3061 atgacttgta aaaaggtact gacactacca aaaagcgtac ctaaaatcat ccctgacatc
     3121 agtaataaaa aaagattcgc tctcattttt ttcaaaagaa agaaaaataa caatagactc
     3181 agcccgacca tcagcccaac gttcaataag aaaaaaactg tacctgtctg ctgaagcatc
     3241 gtcactcctc cgataaagaa gaaaaggacc gtttggacaa ggacatataa cgactcaagt
     3301 cccaatactg aaggagtcaa aaactggtta tgcgtaattg tctgaaagct gattgtggca
     3361 ctgctgacag ctagcgagac aaggataaat gcaaaaattt tcttactcct caatgcacat
     3421 gcaaaatgcc agtttccata tgtatggatc gtcaagtaca ttcctgtaac aaataaaaca
     3481 agaacaagca taccaataaa aatagatggc ttttttttaa gcatatcgtc gtccccttat
     3541 cagaagataa aggaagatag ccccaccgat gattccaacg atcacactga ctggtatttc
     3601 ataaggaaaa atcaacacgc gagcgacaag atcgcaaaat actaagaaca aactacctgc
     3661 taaagcagtt acgccaattg ttttgtgcac ttgatctcca tatattctag agacaagatt
     3721 cggaatgatc actccgataa aaggaatgct tcctaccatg atcaacatga ctgcactagc
     3781 caatgatact aagccaatgc ctaaaaactg catcttctgg taatctattc ccagatttac
     3841 tgcaaacgat tcgcccagac caatgatcgt aaatcggtaa gcaaatagcc aaaccaagag
     3901 caacaatgga acaacagcat acaataactc ataatttcct cgtatcacag tcgcaaaatt
     3961 cccttgaagc catgaagtca tattttgtac caactggaat tgataagcaa aaaaagtagc
     4021 aactgaacca atgatattcc ctaacatgac tccaatcaac ggaagcatca attgattttt
     4081 ctggaggaac aatcgcgtaa gggataaaaa aagcaatgtt cctgcaaaag caaagcaaaa
     4141 tgctgtcaag gatcggacaa tcaaggatgc accaggtagg aaaatcatag ccactaaaat
     4201 acctaaacgc gcactatcca tcgtgcccgc agtcgttggt gaaacaaatt tattttgggt
     4261 caaatgttgc atgatcaacc cacaaatact actggtcgct cccacaagga tcaaacaaat
     4321 cgttcgtggc aaacgagtca ctagcaagat ttgtaagctt tgcccatcta actggagcag
     4381 ttctgacaat tgtacattcg atactccgat gaacaatgaa atactactta aaataactaa
     4441 taatgctact agtcgtccgt agcgcatcca atccaccttt ctaattgaga atgattttca
     4501 ttcttatttt aagtgaatcg ttgaacaaaa gcaaccgctt tttcatatgt tttaggtatt
     4561 attgggaata tgctataatc gtaagaaaga caaagagaag aaggagggta ttttttatgg
     4621 cacaagatga tgtaaaaagc catttggata agggacttta tggtacacct ttagtgaacc
     4681 ctgaagaaca gcataaatat ttagggacat tcagggaacg ctgctatctt agtatgactg
     4741 ttgctgaaat gatccaacca aaaaacaaag aaaacttttt aaaagaactc aaaaaacatc
     4801 cagatgcttc catactcttg aatggggctc tacccctttc tgtccaaaac caatatatcc
     4861 agctagcaac gaaaaacaat agccgtttca ctgtagtcaa cgattacgtc actgcaaatc
     4921 ctgaggaatt cggattgctc ttaacagcaa aagaagcagt aaatgaacct gtgattgata
     4981 tcgaaaaaaa atacccagaa gaaaactctt cgccagctga aaaagaaaaa ccaaaaaaga
     5041 aaaaaagctt ctgggatcgt ttattttaaa acgaaaaaac acataaattt ttacatgcaa
     5101 aaaaagcttt tgatcagatt gacatcaaat aggacaatct gtcaaaagct ttttctttta
     5161 aaatacttca ggatataaca atttcaatac attcatcgtc aatcttctgc cttttcctgc
     5221 aaaaggataa acatgcatat gcatggctgg attgaaattt gcttcgatga tcccatatgc
     5281 ttttttatca gttgtaggat cgatttcttt atctggtata atcaaatcga taccactaat
     5341 cttagctcct agtgcttcta ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc
     5401 atcagtcata tcgatcgaat caccacctgt actgatattt gagttctccc ttagataaac
     5461 aatttgattg gcttggggaa ctgattcgat cgtcaaccct tgttctttca acatcaattg
     5521 ttctaattca cctaattgga tcaattctaa aggtgcacga tgattcgttc cacgcaaagg
     5581 gtcactattt ttttcttcaa caagttcttt cactgttcgg atactgtcac caataacatt
     5641 agcagggact cgcaacatga tggcttggac ttctccgtcg atcacaaaga aacgatattc
     5701 cgttcccggc ataaattctt ctactaaaac cgaactgtct tcacgaaaag caatggctaa
     5761 acctgcttga taatcttcca gactagctcc ttccttgaaa atcgtgatac ctaacccgta
     5821 attggttgat ttaggtttga tcacaaatgc ctgctcggca aatctcggat aagcttttac
     5881 cgcttcttcc atggatgaaa attctgcgcc tcctggtacc cggaacccag cttcttttaa
     5941 gacttttttc gttactgttt tgttttccat gataagtggg acgatatagc tgtccttact
     6001 tgtcatgttt gcatttttca catattctac atggtcttgg aattgcagac gcaaaaattg
     6061 atccgactca tctaatactt tgacttggac acctttttgg atggcatcga acataaatat
     6121 ctgagtagaa agttccattt cgcgaaaacc ggccaactga tagggacgtt catggctttc
     6181 tttataatat tgatgtccca atgccacagc aacttcttgc tgactcgata gctgtgcttc
     6241 ttgatagagt ttcgctgcta acgtttgttc aggatgatcc atttgagtca atgcttcctc
     6301 tactaaataa agagaatcca accaatccaa ttcaaccagc atttgcttca ttccttcaag
     6361 aatttctcta ccttcttgat gaaattctgt ttgatctgat ggttgttcta gtgctacttt
     6421 gttatttcga gtcgtacctt ctgctaccca ttggtcacag tcttcttttt cttctgtcca
     6481 taacatgtac atcaggaata attgtaagaa ccgaacttgc tctggattga tacccaatct
     6541 tgcataagga ttcaaatcga tattccgcaa ttcaatataa cgaacacctg ttgttgctaa
     6601 atctgcgact ttctttccgc ctcggaaacg aagcggtgta taaaattctt tctcctccga
     6661 aagtttccct tcttcgatca ttcgctcgat atctgctaaa tactgtttca tagaagcata
     6721 ggaaactttc acatttggat gattcgtata gcccagcgca ctgttgcgaa tactgcgaac
     6781 aggttcttgc ggatgtgatt catctaaaaa gtagttcttc tcactcattg gtgaagcacc
     6841 gaataaataa gtgatcatcc agcgatagcg cataaagttt ctcgctgttt tcaaataaag
     6901 ttctgttttg aattcggaaa aatcttgaaa ttcttcctga tggctgaaca atgttcttag
     6961 caactcatcg ccaaattcaa aattgaaatg aataccgctt accatctgtt ttcgcttccc
     7021 gtattctttt gctaaataac gtcgatacaa gacatcttcg aaatttttta attttgcaat
     7081 aatgatctct tcgtcttttt ccggtaaggc aggtggcata cttaatggcc agatcatctc
     7141 ttctttcggt atagaacgag cagtcacatc ataaacagca gcgagatact gaaataattc
     7201 gggaatagaa tcggtaacag gggtgatcat ctctgtttgt gtttcgctaa aatctgtttg
     7261 gatatagggg tgataggatc gatcgccaaa gattgctgga tgatcggttt ttgctagatt
     7321 tcctgcaaga tcaacccgct gtccttcccg ttctatacca aatcgagctt ggtcgataaa
     7381 agggcgcgca ttgacatgca ataataattg cttaaaattc atcattaagt gacctccaat
     7441 tcatatgaat ctaattataa tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc
     7501 gcttacaaat aagtataaaa atgaaggcat caaaaacaca gctatgttaa aaacaatcat
     7561 tcaaacaagg agctgcaact tttgtcacag ctcctgcttt ttagacatgg gttattcttc
     7621 ttttagtaat cctttgaaca atccaacgac aaaatcattt ggatcaaacg gctccaagtc
     7681 atcgattcct tcaccaagac cgaccaattt cactggtaaa tgcaattcat tacggatagc
     7741 aagtacgata ccgccctttg ctgtaccatc taattttgtc aaaacaagac cggtcacatc
     7801 tgttgtttct ttgaattgtt ttgcttgaac catcgcgttt tgaccagtgg ttgcatctaa
     7861 tacaagaagt gtttcttggg gtgcttctgg taattcccgc tggattactc gttttatttt
     7921 ttctaattcg ttcatcaagt tcactttatt ttgcagtcgg cctgcagtat caactagtaa
     7981 aacatctgcc tgctctgtct ttgctctttc caaagcatcg aatacgacag ctgctggatc
     8041 gcctcctgca ttgccacgaa caacttcaac accagcgcgt tcgccccaga caactaactg
     8101 gtcgattgct ccagcgcgga aggtatctgc tgctgccatc aatacttttt taccttcgtt
     8161 tttgaactga tgggcaagtt taccaatact tgttgttttc cctacaccat taacaccgac
     8221 aaacaaaata actgttagtc cgtcttcttg gatatttagt tcatttactt cattgattcc
     8281 ttccgcttca tagagatcca caagtttctc gatgatcgca ttttggactt ctgccgattt
     8341 tttcgcattt ctgagtttca cttcttgacg taatgcttcg gtgattcgta tcgctgtttc
     8401 aaaacctaca tctgcaccga taagtgtttc ttctaattct tcaaaaaagt cttcatcgac
     8461 tgttcgaaaa ttggcaaaca actcattcat ccgttcacca aatgttttac gcgtcttttc
     8521 taaacccttg tcgtacttct cctgtacttc aacttcttct gttttctctt cgccagtaaa
     8581 agcacgcttg attttatcaa aaaatcccat ttgactcccc ctactctaat acaaattttt
     8641 ggatcacttt ggctacgcca ttttcttcat tgctggctgt cacgaaatct gctgcttctt
     8701 tgacgaaagg cacagcattc tccatcgcta cgcctagtcc agcatattgg atcatcggta
     8761 aatcattttc ctcatcaccg atcgccatga tttcttcttg tttcaaacca agatcttttg
     8821 ccaataaaga aataccataa gcttttgtaa caccttttgg cataaattcc aaaagcatac
     8881 ttcttgtttt gatgatctca tacatttcat ggaattctgc tggtatttct ttgattttag
     8941 gatctaattc ttcttggtca taagcgatta ctgccttatt aagcacaaat tcatctgtaa
     9001 tatcgcttat atccgctggt tggaattgca gcagcttatt caacacattg tacaaggatt
     9061 gcttccttgg ggcactaggc agctgtaaaa ccacattgtc agacaaaaca tctagtggca
     9121 gatccaattg ctgagccagt ttgtataagc gttggatatc tgatacttcc atcactttct
     9181 tttccatgat ttcaccagta tcattttttt gtaccaaacc gccgttgaaa gtgatgctgt
     9241 agtcaccttc atctaccagt cctaattctt gcagataatg cgccatcgca gctaaggggc
     9301 gtcctgtaca tagaacgatc ttgacccctt gctgttttgc ttgtgctaat gcttgtttat
     9361 tctcatctga aatcagcttt tgttcattta ataatgtacc gtccaaatcg attgctacta
     9421 atttgatcat tttcgttcct acactttcgt ttcgttgatt ttcgcctctt cattgatatc
     9481 ttccatccgt acagaaacga tttttgatac accggattcc tgcatcgtca ctccgtacag
     9541 tacatctgct gcgaccatcg ttcctttgcg gtgtgtgact acgataaatt gtgtgtcatc
     9601 ttggaaatcg ctcaagtaac gaccaaaacg tttcacgttg gcttcatcta acgctgcttc
     9661 tacctcatct aagatacaaa acggaacagg acagacacgg ataatggaaa agagcaacgc
     9721 gatggctgta agtgctcgtt ctcctccaga aagcaaactt agactttgca gttttttgcc
     9781 aggaggttgt acttcaatct caatacctgt tttcagcaaa tcagttggtt ccgtcagtac
     9841 taattcagct ttaccgccgc caaacatatt tggaaagaca attttgaatt cttgacggat
     9901 agcttcaaat acttctttga atcttgtccg cacttcatca tccatctcat ccatcgtttc
     9961 aaacaattgg tttttcgctg ctaacagatc atctcgctgt gttgctaaga atgtatgtct
    10021 ttcactgact tgttcgtatt gttcgattga atttaagttt actggtccca gtttttcaat
    10081 ctgttctttt aaggatgaaa cctttgttct actgtcttcg atatcggttg tttcttgata
    10141 gtcttgggaa gctttctcaa atgtcagctg atattctgac tgcagatagg tcaaatgatt
    10201 atctaaaatc atttcagctc ggtctttttg gacttctaat ttggattgtt ccgacagtcg
    10261 tgctttttgc tcacgattac gctctgccaa gactgcttca agcttatcga tctccttttg
    10321 ccttctatct cgctgctctt tggcttttgc caactcggct tttaatgttt ctcgctgagc
    10381 agataattcg ttaatttgtt tttccaaact ttcttctgtg acttcatgat cagaaaagtc
    10441 tgctgtcaat gtcgccaact gcttttctat cgcttcttga cgttcggttg cctctgcttt
    10501 ttggactcgg gcaccgcgga gctggatctg cagatgatta aactgctctt tcaagacagc
    10561 aagatctgct tgttcttgtg ctttttgact ttgtacttgt gctcttctgg cttccatctg
    10621 gtcgctttct tgacttaacg atttgatttc ttcgtcgatt ttttgtcgtt gcgattcaag
    10681 atctgtttgt ttgtctttca attcgttcgt ttgcttttcg taatcttcga taaactgctg
    10741 cagttcacgt gtctcaaaag ttgagatttg cttttctttt tcaaaacgtt caagttcatt
    10801 tatgatgttt tgtagctgat ttgtcgcttc ttgttcttca aaacgtaatt gttcgcctcg
    10861 tgtacgcaaa acttcttgtt cttcggctaa acgagctgtt tcttgttgca gctcctgaac
    10921 ttttttttct tgtgcttgga gttgtttatc tgcttcttca aattcactag tcaactgttt
    10981 caattcttga ttttgtacaa acaaactgcc ttggtttcct cgtttattcg ctccacctgt
    11041 catggatcct ccggcattca tcacatcacc ttctaaggaa accactcgat attgatagcg
    11101 gatcgtttgc gcgattgcat tggcactagt taagtctttg gctaaaagga tcgttcctag
    11161 caggttttga accacagttt ggatttgatc gggataactg acttgttcac tggcaatccc
    11221 tataaaccct tctacagctg ctgcttgtgt caatatatga gccggtaatt gtcttggttt
    11281 gattgtcgtc agtggaagga atgttgctct tcctcctctt tgctgtttca aatacgtgat
    11341 ggcttgccga gcatcttttt cattttcaac gatgacatgc tgggctgccc cacctaaagc
    11401 tgcttcgata gctaacgtga aatctgcagg aacatcgatc agttcagcca ctgctccgac
    11461 aatacccgaa agctgctgtt tatgctgcaa gaccaatcga accccttggt aaaaaccaaa
    11521 atagttttct tggatttctt gaaggctttt cttacgagca cgtagctgct ggacttgatt
    11581 cattaattga tacattttag gctgttcttt tgctaatgct tcttggaact ttttcccgtt
    11641 ttgttggatc atttctaact tctcttttgt ttcagttaaa gatgatttta gctgcgcctg
    11701 cttttgtgtc aattcttctt tttgtaagat caatcggtct actgatgctt ctacttccga
    11761 ttgcttggct aacgtctgct tgctttttgc tgtttcttgt atatattgac gttctagata
    11821 cttcagttcg tttcctacag ctgcttgttc ttgcatcaaa tcaacatatt gatctcttaa
    11881 ttctgccaat aattctttcg ttgatttttg gtatttttct aattcttgct gtgtttttat
    11941 gagatttgct tcagcttttt ggatctctgt ttctttttct gctgctgctt tcatcaagct
    12001 ttcttgaagc ttttcaaaat gtttaacctt tttttgtgct tctgctaatg aagtttgata
    12061 ttcttgcgag cttttttgtg tatgctttgt ccgctcctgc aaaacatctt tttgtccttc
    12121 tgtctgcttc agtttttccg aaagatctaa taatacttgc tgattttttt cgatcagacg
    12181 atcggcttgc gcattttctt tacgctgctt cgccaaaatg gattcttgtt cttggatcga
    12241 ctcagatagt ttgcctaatt ctagattgaa tttcgcaagc tgtgcttgtt tgttgtccca
    12301 atcttttttt gctgtcttga tttcagcaac catcaaagag acatcggttt gagtcagtgt
    12361 ttcttttagc cgtaaaaact cctttgctgc ctcactctgg gcggccaaag gagttaactg
    12421 ttcttccaat tcgtggatga tgtcttggac gcggctgagg ttgtcttctg tttcaaaaag
    12481 cttctgttct gctttctttt tccgctgctt gtatttcaag acaccagctg cttcttcaaa
    12541 gatcccacga cggtcttccg gtttactact gaaaatcgct tctacttttc cttgagaaat
    12601 gattgagaac gattcttttc ctaatccaga atctaagaac aattcttgga tatcttttaa
    12661 tctgcagctt tgtttattga tgaaaaactc gctttcccca gtccgtcgat aacggcgggt
    12721 tacactgatc tcattgtact ccaatggcag ataatgatca ctattgtcta aaatcactgt
    12781 gacttctgca atattcagct gcttacgggt atcggaaccg gcaaagatga tatccggcat
    12841 ttttccgcct cgcagacttt tagcagattg ttctcctaat acccagcgga tagcctctgt
    12901 aatattgctt tttccgctgc catttggtcc aaccactgct gtgacgctat tttcaaaatc
    12961 aatgactgtc ttgtctgcaa atgatttaaa accggctatt tcaattcgtt tcaaatacaa
    13021 agctttccct cacttacttt tacagtgctg ccaatgcatt ttccgccgca gcttgttctg
    13081 ctaatttttt cgatttccct tgtccagtgc caatcaattg atcatctaca taaacttcga
    13141 tccaaaatac tcgttcatga gctggacctt cttcattgat caatcggtat tcgattgata
    13201 catcacctga tttttgcaag acttcttgta gttttgtttt atgatccatc tcatgtgaaa
    13261 aagcacctgc tttgacttta ggaaaaatca ctttttcgat gaatgcatgg gtcgtgtcga
    13321 atccctggtc aagatataac gcgcctaaaa aagcttcgaa taggtcgcaa agcaatgctg
    13381 gtcgcgtacg tcctcctgaa ttctcttctc ccttgcccaa caaaacatat tgatcaaagt
    13441 gacattcttt ggcaaatttc gacaagctgt cttcgcgaac gatcgcagct cgtgtttttg
    13501 tcaattttcc ttctggcaaa tgaggatata aacggaataa atactgagaa accatcagtt
    13561 ctaaaacggc atctcctaaa aattcaagtc gctcattatc cgataattgc agattgcgat
    13621 gctcattcac ataggatgaa tgggtaaaag cttgttcaag caaatttaga tcgtgaaaaa
    13681 cgatgttata gttttctttt agttcttttg tcagctggtt gtccattttc cacattcctt
    13741 ttatggatat tttttcgata cttccttatt atactggttt tttgtcaaat ttgaaatggt
    13801 tcggtcaaaa ggtaagtaaa gctttaataa tcctagtgat tgacgagaag ttgtgaaaaa
    13861 ttcgtttagt ttcgagtatg aagaaaaaat tttgtgaaaa tggctttcca tatttttgca
    13921 taaatttgac ttaataccga gaaactggac tttgaacacc gtttattgga gaagtcatga
    13981 aatcagcgcc ttgacctgaa tattagagaa ataaacagaa aaatatcaaa aaaccgaaaa
    14041 aggacatcgc cctttttcgg tcatttttag tgattatgaa cgtgaatctg ctgtaacaga
    14101 aacatttgcc catgggtttg gtacatcata agaccaatcc cagtctttga cacggtcgtt
    14161 gactggcaat acttcattac ggaacaacgt tggaatcgca aatgcttctt caaatgcata
    14221 ttgttgccat ttttgatatg cttctttttg gtactcttca tcaaatgctt tgcttgaact
    14281 gatatcgttg atcaattttg tattttcttc tgattcataa cgagtgtagt tgaacgcagc
    14341 atttggtccc catagtccac ttggatttgg atcagttcct gtattccaag cagcttggta
    14401 aacatcaatt tcaggatcat cgttttctaa tttatcatag aaagaattga attcgatcag
    14461 acgacctgtt gtgtattgga catccaaacc aatttctttc cattgttgga tatagtaatc
    14521 tgccaatggt tgcgctgttt ccccaccgga catcgaagca aagttgattg ttagtttatt
    14581 tccatctttg tcttctctta aaccatcgtc atccacatct ttgtagccag catcatctaa
    14641 tagttttttc gctttgtcta gatcttgtgt gaatcctttc atctcatcat catggtatga
    14701 ctggaatact ggaggaatca atgaagtcgc attgcttctc aaacctgcat agaaacgttc
    14761 acctactgca ttattatcta atgcatagcc cattgcttga cgtaatgatt tatcagccat
    14821 ttttgaatct ggattgtatt ccacactctt tgtatctgca ttccatttac ctaacttgaa
    14881 tcctagataa gtataagaag tttgttcacg gcctaagttt gtatagccag caatttcttt
    14941 ataggtatcg taagtatctg ttggcatttg gaaaatcata tcatataatt tagagttcaa
    15001 tgcttcagtt gcacttgcca tcggtacaga tttcatcgtg atctttttca atttaggcgt
    15061 accattccag tagtgctcgt ttggcaagta ttctactgat tctcctgcaa cgatattact
    15121 catgtaataa gggccaaaag tcactggatt tttacgtact tgatcgcttg attccatatc
    15181 tttgattgct acaccttcta acgtatgttt tggtgctgca tatgaccaga taccaccacc
    15241 cgattgaagc atgcttgggt ttacttcttt atattcgatt tcaactgttt tgtcgtctat
    15301 ttttttgatt ccggagattg tgtcggcact tccatcatga tattcttcca tacctacgat
    15361 gttacggaaa gtgtcgtcat aacggatacc agtgtaatct ttattcccaa tgatttcgta
    15421 agggaaaatc acatcgtccg ccgttacatc ttcaccatct gaccatttga cgttgtcttt
    15481 tactttgatt gtcgcttttt tcgtatcttc atctagatca agcgttgcag ctccgtcgtc
    15541 tgtgattctg aaatcgtcat cgtttgcaaa tagcggttca tgagaaggag ccataaaagc
    15601 tgcatcatat gaatcttggt aaaactccca ctggaacaat cctttaaact gactgtccgt
    15661 tgcgaccgca gcttctaatt cgccgtcttc gattgcatca tcattatttt ttgttgcaat
    15721 tggaaaagtt aattcctctt tgttattgcc gttgcctcca gagttgtttc cagaggtatc
    15781 tgtacctccg cccccgcatg ctgcaagaac gacagcagac atcagcgctg caaatccaaa
    15841 aatcgttttc ttcattccta tttccccctt cgcttctata ctagcctaac cgttggcgcg
    15901 catctgcaga acgcttgaat gcttgtccaa cataattgat actcaacatc aaaactaaaa
    15961 taaggattga tgcgggtaac cagatccatt gccgattcac taagacttct ccgttacttg
    16021 cataaccgat caatgtacct aaactaggta catttgccgg caagccgaaa cctaagaaag
    16081 tcaaagttgt ctcgataccg atgtttgccg caaagttcat cgtcaaatta gtaatgatta
    16141 atgaacttag attcggcatg atttcgcgga acatgatttt aaaatcattg gttcccattg
    16201 tttttgaggc actgacataa tctcttcttg cttcagataa tgtcttgctt cggaataatc
    16261 gggctttagc tacccaataa aatgcactca tgatgaatat aaaggaccag acattgtatc
    16321 tggaaataat cgatacgaag acgataatga tcagcatgat cggtagaatc atgataaaat
    16381 caacgatccg catcaagatg ttgtcaatga tccctccgta gtagccagag atgatcccta
    16441 atccaacccc gatgatcgaa gtaaggatcg tgatcgcaaa tccgatcgta acagagtttc
    16501 tagcacctat gatcaattgt cctaatacat cacgtccgcc ttcatctgct cccagaatga
    16561 acttttgtcc ttgtgtcgaa actgttcctg gtgcagcgta tttgtcgaag atactaacat
    16621 acatcacttt gtcttgatcg gtcagcatcg caccgataaa gatcactaat aataaaacca
    16681 ctagcaaacc taatgaaaaa atagcaattt tatctttttt aaattctcga gcaatcattc
    16741 gtatccccat aggaggaata ctttcttgaa ctgctgtatt gtttttttca ctcatcgatc
    16801 cattctcctt tctgattggt tttattgaat ccgaatacgc gggtcaacga tactcatgat
    16861 gatatctgaa agtaatgtcc ccactagcgt cgcgattcct aaaatcaata ctaaggatgt
    16921 gatcacggta tagtcccgtt ggccgatgga atcaataaat agttttccga ctcccggata
    16981 accaaaaatc ttttcgataa ctacagaacc agcaatcaac gaagtgattt cataccccat
    17041 ttgagaagcc atcggcaacg ctgcatttcg gaaaatatgt cgactgaata ctttattcgt
    17101 cggtacccct ttggaacggg ctgttctgac aaaatcgagc gatttcgaat cgattacttc
    17161 acttcgcaga tattggattg tgacggcagt cgccaaaaat gcttgggtaa ttgatggtaa
    17221 cagaatatat ttcaaacggt ctatccattg agtgataaat ccaccttgca aacctagtgt
    17281 ttgtgatcca cttgttggga accagcctaa acgataacca aaaacaaata gcatgatcaa
    17341 gccgaagata aataaaggaa cagcaaaact aaagaagttg tagattacga caatcttatc
    17401 caagattgaa ttttgatacc ggccagcaag catccccaga ggcacagcaa tcaaataagt
    17461 aaggacaact gtcaagatcg atagatataa agtgttgatg atccgttcgc cgatcaagtc
    17521 agcaacagag cgtttgtata agaaactttg accaaaatcg ccttgcaaag catttccgat
    17581 ccaacggaag tactggacat accatgggtc attcaagcca gctgcttgtc gcatctgttc
    17641 gatcactgcc ggatcctgat ttggattgat caaaccggta aatggatccc ccggcatcgc
    17701 ttgggctaat aagaagatca gaacacttaa aataataact tgcgggatca tcaataaaac
    17761 acgtcgcaga atcgttttcc acattagctt gcacctccat ctttcaacgc cactttgtgt
    17821 gtagttgtga tcgtacgcaa atcgtagact cgaccagtcg catcatagta ttctttttgg
    17881 ttttggatat actcttgttc tactcttcgt cgattttctt tgtggctttc cctattttct
    17941 acatcgattt gcggaatagc tgaaagcaga cgtttcgtat agatatgtcg cgggtcatta
    18001 taaatgtctt ctcttgtacc gatttctaca aatcgacctt tgtacataat tgcgatattg
    18061 tcgcacatgt gttttactac acctaaatca tgtgaaatga ataaatagct gaggccgtat
    18121 tcttgctgga ttcgtttcat gaaattcaat acttgcgctt gaacagatag atccagagca
    18181 gatacaggtt catcggctac gatcagtttt ggattggtag caacagcacg ggctactcct
    18241 agacgttgac gttgtccgcc tgaaaattca tgaggatatt tatatagcgc atcttccggc
    18301 atcccaacga tatctagcag tccttttacc tttttctttt cttcttgatc gcttaaccgt
    18361 tcaaaatttc ggatgggttc agcaatgata tccaacactc gctttttagg attcaaactg
    18421 gacattgaat cttggaagat catctgcaca tccttgttat aacccatttt tttgcggttg
    18481 cttcgcttcg ttacatcttt tccttcatac atgatttgtc cggctgttac cttttctaat
    18541 cccacaacgg cttttcccgt agtagatttt ccagagccag actctcccac taaaccatat
    18601 gtctttcctt tttcgataat aaaatccaca ccatctacag catatacatg atccgttact
    18661 cgattgaaga atccactgcg aatagggtaa tgaactttca ggtctttgat tgtgattaat
    18721 tctgtcatct acacctctcc tttttcgtct ctaaagtgga aatgcttata gcaggagcaa
    18781 cgtaccaagt ggttcggtgc gacttcatga aggataggct cttcttcatg atcgcttgct
    18841 ggtatccatg gaatacgtgg tgcaaaccga caacctttac gaggcatgtt tttcagagaa
    18901 ggaacgacac cttcgatcac atggagttct gcatcatgat ttccttcttg tggtatagag
    18961 ttcagtaaag agcgtgtata aggatgctta ggatgatcaa acagttcttt tacacctgct
    19021 acttcaacga actgtccgcc atacatcact gccactcgat cagccatttc tgctacgact
    19081 cctaagtcat gagtgatcaa gatgatccct gtttttgttt cttcctgaag gtctttcagt
    19141 aaatcaagaa tctgtgcttg gatggttaca tctagggctg tcgtcggttc atctgcaatg
    19201 acgattggag gtttgcaggc gattgcgatt gcgatgatga cacgttgtct cataccgcct
    19261 gatagttcat ggggatattg gcgtccaact ctcgcaggat taggaatccc tacttgttct
    19321 agcaattcta gtgcacgagc ttgacgctgt tctgcattca tctttgtgtg ataggaaagt
    19381 ccttctttga tctggtcttc gattctcatg agaggattta atgcagacaa cggatcttgg
    19441 aaaatcattc cgatatcatt tccgcggatt ttattataga gcgtttcgtt caggtcgatt
    19501 aaattcagat cgttatataa aatctcacct gtaatttttg tgttattagg atcaagtaat
    19561 cccataatcg tagtagccaa ggtacttttc ccgcacccag attccccaac gattgctaaa
    19621 atctcgtttt tgtctaaagt aaatgaaacg tcatctactg catcgtaata gtcatctttg
    19681 agtcggaatc ctgtatgtag gttctgaact tctaataatt ggttatcgga caatcgaata
    19741 cccctctctg cttcctaaat ggaagttttt cttaataaaa aaggacatta cacagaataa
    19801 atcgttttat aacaaaggtt attcttatct tt
//
LOCUS       DANKAY010000051        19158 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000051 DANKAY010000000
VERSION     DANKAY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            30..1457
                     /locus_tag="PWY98_001720"
     CDS             30..1457
                     /locus_tag="PWY98_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2037471.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1549..1914)
                     /locus_tag="PWY98_001721"
     CDS             complement(1549..1914)
                     /locus_tag="PWY98_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2037472.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1911..2276)
                     /locus_tag="PWY98_001722"
     CDS             complement(1911..2276)
                     /locus_tag="PWY98_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2037473.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2468..2716)
                     /locus_tag="PWY98_001723"
     CDS             complement(2468..2716)
                     /locus_tag="PWY98_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037474.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2715..2843
                     /locus_tag="PWY98_001724"
     CDS             2715..2843
                     /locus_tag="PWY98_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2037475.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2900..4594
                     /locus_tag="PWY98_001725"
     CDS             2900..4594
                     /locus_tag="PWY98_001725"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2037476.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4598..4717
                     /locus_tag="PWY98_001726"
                     /pseudo
     CDS             4598..4717
                     /locus_tag="PWY98_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4694..4867
                     /locus_tag="PWY98_001727"
     CDS             4694..4867
                     /locus_tag="PWY98_001727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037477.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5245..6378
                     /locus_tag="PWY98_001728"
     CDS             5245..6378
                     /locus_tag="PWY98_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2037478.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6409..6729
                     /locus_tag="PWY98_001729"
     CDS             6409..6729
                     /locus_tag="PWY98_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2037479.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6758..7222)
                     /locus_tag="PWY98_001730"
     CDS             complement(6758..7222)
                     /locus_tag="PWY98_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037480.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7354..8607)
                     /locus_tag="PWY98_001731"
     CDS             complement(7354..8607)
                     /locus_tag="PWY98_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2037481.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8636..8953)
                     /locus_tag="PWY98_001732"
     CDS             complement(8636..8953)
                     /locus_tag="PWY98_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2037482.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8979..9299)
                     /locus_tag="PWY98_001733"
     CDS             complement(8979..9299)
                     /locus_tag="PWY98_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037483.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9763..11178)
                     /locus_tag="PWY98_001734"
     CDS             complement(9763..11178)
                     /locus_tag="PWY98_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2037484.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11195..12511)
                     /locus_tag="PWY98_001735"
     CDS             complement(11195..12511)
                     /locus_tag="PWY98_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2037485.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12661..13359)
                     /locus_tag="PWY98_001736"
     CDS             complement(12661..13359)
                     /locus_tag="PWY98_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2037486.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13648..14604)
                     /locus_tag="PWY98_001737"
     CDS             complement(13648..14604)
                     /locus_tag="PWY98_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2037487.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14717..15550)
                     /gene="msrA"
                     /locus_tag="PWY98_001738"
     CDS             complement(14717..15550)
                     /gene="msrA"
                     /locus_tag="PWY98_001738"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2037488.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15725..17359)
                     /locus_tag="PWY98_001739"
     CDS             complement(15725..17359)
                     /locus_tag="PWY98_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037489.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17346..19034)
                     /locus_tag="PWY98_001740"
     CDS             complement(17346..19034)
                     /locus_tag="PWY98_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037490.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6081 a         3747 c         3188 g         6142 t
ORIGIN      
        1 gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt
       61 taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc
      121 tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa
      181 aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt
      241 tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt
      301 taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat
      361 gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta
      421 atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc
      481 tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat
      541 cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt
      601 atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc
      661 aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt
      721 acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag
      781 aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt
      841 tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa
      901 aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt
      961 tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc
     1021 cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa
     1081 ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc
     1141 gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct
     1201 atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa
     1261 ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt
     1321 atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg
     1381 tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag
     1441 ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa
     1501 gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg
     1561 gacatctacg ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac
     1621 caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc
     1681 agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc
     1741 attgttctcg ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa
     1801 atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc
     1861 cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc
     1921 tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact
     1981 gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact
     2041 gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc
     2101 agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac
     2161 caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa
     2221 atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac
     2281 gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt
     2341 cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc
     2401 ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa
     2461 ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac
     2521 ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat
     2581 ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc
     2641 gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca
     2701 aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg
     2761 cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat
     2821 atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga
     2881 agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc
     2941 caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac
     3001 cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga
     3061 tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg
     3121 tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc
     3181 tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg
     3241 aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact
     3301 gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga
     3361 agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt
     3421 tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc
     3481 tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct
     3541 agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga
     3601 gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt
     3661 caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact
     3721 gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg
     3781 gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga
     3841 ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa
     3901 tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac
     3961 gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc
     4021 cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat
     4081 gtctattgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat
     4141 ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa
     4201 aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg
     4261 accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc
     4321 tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa
     4381 agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta
     4441 tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt
     4501 tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac
     4561 tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct
     4621 gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga
     4681 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaagctgc
     4741 cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt
     4801 agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagg
     4861 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
     4921 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
     4981 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
     5041 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
     5101 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
     5161 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
     5221 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
     5281 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
     5341 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
     5401 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
     5461 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
     5521 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
     5581 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
     5641 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
     5701 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
     5761 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
     5821 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     5881 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     5941 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     6001 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     6061 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     6121 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     6181 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     6241 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     6301 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     6361 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     6421 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     6481 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     6541 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     6601 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     6661 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     6721 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     6781 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     6841 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     6901 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     6961 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     7021 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     7081 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     7141 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     7201 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     7261 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     7321 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     7381 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     7441 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     7501 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     7561 gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt
     7621 caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat
     7681 cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa
     7741 cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt
     7801 gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc
     7861 tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa
     7921 ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc
     7981 cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc
     8041 agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga
     8101 agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc
     8161 atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa
     8221 caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat
     8281 cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa
     8341 agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt
     8401 ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt
     8461 attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc
     8521 ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt
     8581 ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg
     8641 cgttcaatcg acgatacaga tcaatgatct cttttgctaa atctgtaaaa gctatagatg
     8701 tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg
     8761 cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat
     8821 ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca
     8881 ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt
     8941 tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat tagtttttct
     9001 gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt gattacatct
     9061 aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc
     9121 ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca
     9181 gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg
     9241 actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt
     9301 ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa
     9361 aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact
     9421 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
     9481 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca
     9541 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac
     9601 tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt
     9661 tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca
     9721 aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc
     9781 aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa
     9841 tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc
     9901 attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc
     9961 ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc
    10021 atcttgcacg attcctttct catccatata ctgttcttca tattctcttc ccataccgtt
    10081 ttctgttatc agccaaggga tattatgata attttctttt agatacatgg ctatatcata
    10141 catgattttc ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc
    10201 aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg
    10261 ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg
    10321 aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc
    10381 caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa
    10441 caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat
    10501 ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac
    10561 tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg
    10621 ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt
    10681 gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt
    10741 agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc
    10801 tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc
    10861 gatcatttca cttatatgct gagcatattc ttcatctact atacagttct cataatcagt
    10921 aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag
    10981 atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc catacccatt
    11041 atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt
    11101 ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt
    11161 tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat
    11221 ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg
    11281 taataactgc accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg
    11341 gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa
    11401 tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga
    11461 tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca agggataact
    11521 tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga
    11581 tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata gtaataatgt
    11641 tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca
    11701 acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt
    11761 gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc
    11821 gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa
    11881 atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag
    11941 cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca
    12001 accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg
    12061 tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta
    12121 attcataaac agttggatta acagataaat aagacatcac tgcgatgata ccagggaata
    12181 caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg
    12241 tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa
    12301 aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca
    12361 atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca
    12421 ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt
    12481 tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct
    12541 ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt
    12601 accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg
    12661 tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg
    12721 gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc
    12781 tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa
    12841 agcaattgat tcattaatga ttgggataat atgcagcaag gctacgtttc cgctagcttc
    12901 tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa
    12961 agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata
    13021 ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg
    13081 tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg
    13141 cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag
    13201 agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata
    13261 ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt
    13321 gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc
    13381 tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtatac
    13441 aaaattgatt aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac
    13501 taggataaaa ttcactccta gccttcgatc atttttattt tgaacgacaa aaaatatgat
    13561 gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt ttttaatata
    13621 tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc tcaattcttc
    13681 ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact gacttaaccg
    13741 acttgctccg accagtacac ttgtaacaga tttttgtcgc aaattccatg caagggccat
    13801 ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga ttttttccag
    13861 agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc
    13921 ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg gactgaacgt
    13981 gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta gtagtgcacg
    14041 agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc tttttaaaat
    14101 ttcagtcatt ttttttgtat cttctccatt atagttggat ataccaatat ataatgcttt
    14161 tccttgtctt acaagttgca tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc
    14221 tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga gactttggtc
    14281 acaacttgaa atgatggatt tacgagagcc ccattcccca tatggccctt cccacatatg
    14341 ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat cttctctaaa
    14401 aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat tcgcaaggtc
    14461 gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat tatggatagg
    14521 atcataatca ccaaaatttt gccataatcc gagagataaa atcggtaatt tcaaacctga
    14581 atgaccggat ttacgatatt ccattttttc ataacgtgca ctatctgcaa tatacaattt
    14641 ttctcctcct catcaaatct ttttaaaacg ctttcttttt tctattttta gtttactaca
    14701 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
    14761 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta taacgtttcg
    14821 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    14881 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    14941 gttccgctaa ttctttttgt cgttcgactt gataaaaaat cactggacga tattgcttcc
    15001 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    15061 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    15121 ctgtgtaacc accacttact tgatcataat ttggtttttc aacatgtccg cccgtgtatc
    15181 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    15241 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    15301 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    15361 caggcgtcag attcattcgg atagctaatg gacgtagtgt tgcttctagt tttgataact
    15421 gttctttaac ggatatattt tctcttatgt cattctttgt ctgaatcaat acttttcgtt
    15481 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    15541 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    15601 tatcataaag tgaggaaata agcgattact tcatccccct acagattttt cgtataaaaa
    15661 agagagaatc cttatcgaca gattctcctt tttaatcaat ttattttata aatgaaaagt
    15721 taatttacct tttatggaaa actattttaa aaaataaaca taaatatgca gttaatgtaa
    15781 caagataaga aataatagtc gttactgtat aacttgtgct tactttaata tcagcaactc
    15841 ctttgactcc gcttatttgt accaatccat tagcattcac ctgctccttt acaggttgat
    15901 gatttatttc tacattcgtt cctaaataag catatacagg aagtgtgatt acttggctct
    15961 tctcattagt tactctatat atgatagaac taccagttgt tttataagga aggtttaccc
    16021 attgactgcc aatctttgct tcgtgtttta ttatatgctc cttattctcc aatgaagcga
    16081 taggagaata atcatataaa aaataatcca ttcctttagt ggtatacgcc tctactgttt
    16141 ctttgtttac aacaatatgt cccggctgag aaattatttt ttgattgaaa ttttttttac
    16201 tagaaacagt taaagtgatt aataacaaaa cagtcataaa taaataaaat agttctcttg
    16261 tcttattgta tcttctattt ttgaaaaata tcatcgttaa tatcagagaa gagaatatta
    16321 tttgcaagct taagatccgc caaggaaatt gtaacaattt ggctggcgta ttttgcagaa
    16381 gataccatgg caccaaacta gttgtagcta gccaagtcaa acacgtacca taataaatct
    16441 ttttttctat gtttgttaat tttttaaata aaatcagggg aatacataaa gataacagac
    16501 caactagtcc cattgagtaa gtagttaagt cgttgttcat agcttcagtc agactatcga
    16561 acacattaga tgcatgagca tataaatttg tcacgctagg tttgttgatt ttttggtaga
    16621 gcatttgttc aaacatcgga taccaaaaag cactcgtcag aaccaatgtc attcctgccg
    16681 cactaaataa ttttttaaat atagcaataa aatctgcatt cttgaaacct ttagaaacca
    16741 aacgaataaa aataaaaatg ataattaatg ctgtactcat taatgttgat agtacatggc
    16801 tgtacactag caaactcata ctcaatgcta gctttacaga cggatacttt tcttcttttc
    16861 cagagatgat ttgataagca taaaataata cagggataac aagtgttatt gcgatagctt
    16921 cggcaattgc ggaacgatag tagatgtcta ctgtccgata agcagagaaa atatagagac
    16981 aactaaataa aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta
    17041 cgagtgttgt acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt
    17101 ttgtcatcca atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta
    17161 caccatatcc agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata
    17221 accccttgat tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat
    17281 ttttgaaaaa gatgaaagaa tatatactac taactataat taatccactc aatattacta
    17341 cttttttatt tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct
    17401 ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga
    17461 atactgatcc ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta
    17521 ttttctccag catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg
    17581 actttaccgt ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt
    17641 tgctttttgt tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct
    17701 ttttcccacg attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc
    17761 gcttctgttg gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt
    17821 gtttcttcat tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct
    17881 tttgctgcta tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa
    17941 tattttttat tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag
    18001 atttggatac ctaagatacg ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac
    18061 aaaaaccatg gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg
    18121 attttttctt ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat
    18181 cctaataacc ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct
    18241 gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa
    18301 ttcatctgct gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca
    18361 gcctttacta aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac
    18421 aaacgaacta taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga
    18481 gtatacacaa gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat
    18541 atcaatccat aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca
    18601 attgcggatc gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta
    18661 aataaaaaag cagcatctat ctttttaaaa aacttttctc cataataaaa actaatcaga
    18721 atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa gttatttgaa
    18781 attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata gttcagtcca
    18841 tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga agataaccct
    18901 ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg agcatttctc
    18961 ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag aaatgctttt
    19021 ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag tatactagaa
    19081 ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg atatactaga
    19141 aaagaatcaa tttttatt
//
LOCUS       DANKAY010000052        18003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000052 DANKAY010000000
VERSION     DANKAY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(39..872)
                     /locus_tag="PWY98_001741"
     CDS             complement(39..872)
                     /locus_tag="PWY98_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2037491.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1176..3341)
                     /locus_tag="PWY98_001742"
     CDS             complement(1176..3341)
                     /locus_tag="PWY98_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2037492.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3601..5895
                     /locus_tag="PWY98_001743"
     CDS             3601..5895
                     /locus_tag="PWY98_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2037493.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5888..6553
                     /gene="pgmB"
                     /locus_tag="PWY98_001744"
     CDS             5888..6553
                     /gene="pgmB"
                     /locus_tag="PWY98_001744"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2037494.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6546..7553
                     /locus_tag="PWY98_001745"
     CDS             6546..7553
                     /locus_tag="PWY98_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2037495.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7575..8594
                     /locus_tag="PWY98_001746"
     CDS             7575..8594
                     /locus_tag="PWY98_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2037496.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8875..10242)
                     /gene="nhaC"
                     /locus_tag="PWY98_001747"
     CDS             complement(8875..10242)
                     /gene="nhaC"
                     /locus_tag="PWY98_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2037497.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(10351..11772)
                     /locus_tag="PWY98_001748"
     CDS             complement(10351..11772)
                     /locus_tag="PWY98_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2037498.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(12015..13892)
                     /gene="tdc"
                     /locus_tag="PWY98_001749"
     CDS             complement(12015..13892)
                     /gene="tdc"
                     /locus_tag="PWY98_001749"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2037499.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(14183..15439)
                     /locus_tag="PWY98_001750"
     CDS             complement(14183..15439)
                     /locus_tag="PWY98_001750"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2037500.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(15551..15756)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            16019..17320
                     /locus_tag="PWY98_001751"
     CDS             16019..17320
                     /locus_tag="PWY98_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2037501.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(17389..17988)
                     /locus_tag="PWY98_001752"
     CDS             complement(17389..17988)
                     /locus_tag="PWY98_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2037502.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         5472 a         3823 c         3260 g         5448 t
ORIGIN      
        1 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
       61 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
      121 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
      181 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
      241 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
      301 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
      361 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
      421 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
      481 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
      541 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
      601 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
      661 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
      721 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
      781 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
      841 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
      901 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
      961 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     1021 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     1081 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     1141 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     1201 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     1261 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     1321 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     1381 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     1441 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
     1501 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
     1561 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
     1621 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
     1681 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
     1741 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
     1801 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
     1861 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
     1921 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
     1981 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
     2041 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
     2101 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
     2161 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
     2221 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
     2281 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
     2341 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
     2401 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
     2461 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
     2521 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
     2581 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
     2641 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
     2701 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
     2761 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
     2821 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
     2881 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
     2941 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
     3001 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
     3061 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
     3121 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
     3181 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
     3241 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
     3301 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
     3361 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
     3421 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
     3481 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
     3541 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
     3601 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
     3661 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
     3721 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
     3781 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
     3841 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
     3901 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
     3961 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
     4021 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
     4081 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
     4141 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
     4201 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
     4261 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
     4321 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
     4381 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
     4441 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
     4501 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
     4561 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
     4621 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
     4681 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
     4741 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
     4801 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
     4861 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
     4921 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
     4981 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
     5041 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
     5101 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
     5161 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
     5221 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
     5281 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
     5341 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
     5401 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
     5461 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
     5521 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
     5581 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
     5641 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
     5701 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
     5761 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
     5821 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
     5881 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
     5941 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
     6001 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
     6061 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
     6121 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
     6181 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
     6241 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
     6301 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
     6361 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
     6421 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
     6481 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
     6541 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
     6601 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
     6661 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
     6721 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
     6781 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
     6841 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
     6901 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
     6961 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
     7021 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
     7081 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
     7141 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
     7201 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
     7261 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
     7321 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
     7381 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
     7441 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
     7501 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
     7561 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
     7621 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
     7681 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
     7741 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
     7801 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
     7861 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
     7921 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
     7981 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
     8041 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
     8101 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
     8161 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
     8221 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
     8281 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
     8341 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
     8401 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
     8461 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
     8521 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
     8581 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
     8641 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
     8701 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
     8761 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
     8821 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
     8881 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
     8941 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
     9001 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
     9061 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
     9121 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
     9181 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
     9241 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
     9301 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
     9361 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
     9421 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
     9481 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
     9541 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
     9601 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
     9661 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
     9721 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
     9781 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
     9841 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
     9901 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
     9961 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    10021 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    10081 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    10141 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    10201 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    10261 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    10321 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    10381 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    10441 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    10501 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    10561 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    10621 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    10681 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    10741 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    10801 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    10861 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    10921 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    10981 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    11041 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    11101 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    11161 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    11221 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    11281 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    11341 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    11401 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    11461 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    11521 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    11581 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    11641 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    11701 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    11761 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    11821 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    11881 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    11941 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    12001 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    12061 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    12121 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    12181 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    12241 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    12301 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    12361 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    12421 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    12481 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    12541 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    12601 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    12661 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    12721 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    12781 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    12841 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    12901 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    12961 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    13021 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    13081 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    13141 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    13201 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    13261 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    13321 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    13381 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    13441 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    13501 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    13561 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    13621 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    13681 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    13741 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    13801 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    13861 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    13921 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    13981 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    14041 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    14101 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    14161 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    14221 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    14281 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    14341 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    14401 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    14461 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    14521 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    14581 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    14641 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    14701 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    14761 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    14821 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    14881 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    14941 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    15001 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    15061 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    15121 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    15181 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    15241 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    15301 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    15361 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    15421 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    15481 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    15541 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    15601 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    15661 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    15721 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    15781 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    15841 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    15901 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    15961 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    16021 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    16081 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    16141 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    16201 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    16261 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    16321 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    16381 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    16441 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    16501 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    16561 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    16621 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    16681 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    16741 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    16801 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    16861 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    16921 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    16981 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    17041 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    17101 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    17161 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    17221 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    17281 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    17341 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    17401 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    17461 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    17521 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    17581 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    17641 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    17701 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    17761 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    17821 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    17881 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    17941 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    18001 ccg
//
LOCUS       DANKAY010000053        17730 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000053 DANKAY010000000
VERSION     DANKAY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(4..915)
                     /locus_tag="PWY98_001753"
     CDS             complement(4..915)
                     /locus_tag="PWY98_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2037503.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1292..1930)
                     /locus_tag="PWY98_001754"
     CDS             complement(1292..1930)
                     /locus_tag="PWY98_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037504.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1934..2704)
                     /locus_tag="PWY98_001755"
     CDS             complement(1934..2704)
                     /locus_tag="PWY98_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2037505.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3122..3538)
                     /locus_tag="PWY98_001756"
     CDS             complement(3122..3538)
                     /locus_tag="PWY98_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037506.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3589..3970)
                     /locus_tag="PWY98_001757"
                     /pseudo
     CDS             complement(3589..3970)
                     /locus_tag="PWY98_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4096..4761
                     /locus_tag="PWY98_001758"
     CDS             4096..4761
                     /locus_tag="PWY98_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2037507.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5158..7191)
                     /gene="brxL"
                     /locus_tag="PWY98_001759"
     CDS             complement(5158..7191)
                     /gene="brxL"
                     /locus_tag="PWY98_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HDL2037508.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7309..9831)
                     /gene="pglZ"
                     /locus_tag="PWY98_001760"
     CDS             complement(7309..9831)
                     /gene="pglZ"
                     /locus_tag="PWY98_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HDL2037509.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9947..13579)
                     /gene="pglX"
                     /locus_tag="PWY98_001761"
     CDS             complement(9947..13579)
                     /gene="pglX"
                     /locus_tag="PWY98_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL2037510.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13643..17314)
                     /gene="brxC"
                     /locus_tag="PWY98_001762"
     CDS             complement(13643..17314)
                     /gene="brxC"
                     /locus_tag="PWY98_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2037511.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17339..>17730)
                     /locus_tag="PWY98_001763"
     CDS             complement(17339..>17730)
                     /locus_tag="PWY98_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL2037512.1"
                     /translation="EVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESIGRLL
                     KMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVPVVMM
                     YPGEYDELSLTILNTNKDDNYYRAFRI"
BASE COUNT         5450 a         3389 c         2513 g         6378 t
ORIGIN      
        1 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
       61 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
      121 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
      181 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
      241 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
      301 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
      361 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
      421 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
      481 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
      541 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
      601 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
      661 ctttgtttga tcatattggg aatcgatttc tttggttgat acgtcatctc catatttatc
      721 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
      781 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
      841 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
      901 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
      961 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
     1021 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
     1081 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
     1141 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgt
     1201 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
     1261 attagaaaaa gtttgttttt tcatttgttt tctaaagcat gatcttcttg tttcctattt
     1321 tggtcagata tggatcatgc gtgaccacga taactaacgt accttgggaa gcctgttgcg
     1381 ataagatttc aacgacttgc tccttatttc gtttatctaa tgatccagtt ggttcatcgg
     1441 ctaaaatgat tttgctattc ttcaataaga ctctcgcaca ggcgattctt tgcttctcac
     1501 caccagataa tgtgtagata ggcgtactta gcttcttgtt tatatttact tttttcaatg
     1561 cctcatactt tgctgttcgc ttatctgaaa catttaaatt cgaaaacttc aatccaatat
     1621 ctaaattaaa atcgattgtt tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga
     1681 gggcatttct ttttatcaag cgcttctctc ttccagttaa atgttttgtt tcttgattaa
     1741 ataaataaaa ttgccctgaa ctgaattgat cattcaatcc taatatattc aataaagtac
     1801 ttttccctgc tccgctctct ccaactacag tcacaaaatc cccagcatcc gcagtgaaac
     1861 gatccaaatg aagaatcgtt ttgtttcgaa tgattttttt cgcacctttt atcactatca
     1921 acggctccaa ttgttaacct ccattcatca gcaataactg acttttcttt tctgtagaca
     1981 aaataaaaac aactgaaaga ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat
     2041 atttaggctc tctttctata agaaaaagga agattacact tgtcgttccc catttgatga
     2101 ggttcatcat gaagaaatct ttatacgttt caaagagcga ataaccactt gaacgaaaga
     2161 tagcgatttt ccgacaattg tagacaaaat atatgcataa catgacatag ctcaacaaga
     2221 agaaagaaaa gaaagtgagt aaaaatatct gtatcaagta atgaaatgat ctgcttaaca
     2281 ctttgacagc tatctcttca gtctgagtat acggaaatga taactgcaag ttatcacgta
     2341 actgactttc ttcaatcagc tcaaataacc tttctttcga caaagagcct atcttgatct
     2401 ttaacggtgg atagatttct ccgctgagaa tatttctttc ccaatacgta ctgtttttca
     2461 atgtcagtat ttcaacaacc ggataatgtt cgatccaggg gatagaagga ataaatgtat
     2521 aaatgggttg attgtttttt ataaatagaa atttcggttc tttttcactg atttcttgat
     2581 aatattctgt tatttgcgcc aggtcatttt ttaaatctgt atgtctatat cgttcaggga
     2641 tcaatataac agggtgcgtt tctttttcgg ttatttggat caaccttcca gagacatcaa
     2701 tcaaaggaaa tttcttaaga taattcagat ttatttttat ccctctgcca aactgttcat
     2761 tttcttcatt caagtaatct gatacattga ctaatatact tccgttttta ttcaaagttt
     2821 catataattc ctgatcggct gctgatgcaa aacgattgga ataaataaaa tccacgtggt
     2881 ttttcccgat ggttactgga taaaatacat gatagtcctc accagcgatt tcttttggtg
     2941 tttcatggga aacagctaac tgtgaagaag aaaaaacgat agttgctaat tcgacattgg
     3001 caacggtcaa aagaagagaa aacaacaatc caaatgtaaa aagaatgtat tggatatagt
     3061 tgaacgcttc tcttaacgaa gcgatttttt caactaataa aagagaaatc aaagagatac
     3121 cctatattcc gctgaacaag aaaacgtaca tctggaacaa ataaagaaat tgctccttat
     3181 aatacataaa aataaaacca ccaatgattc ctgcaattat gaatgtaata atgattttct
     3241 ttttccccaa taaacaccac aatacttgat gtaatgaaaa gccattattc aacaataaac
     3301 tgatttcttt attcatcaat aagaaaaagg atgataacaa aaggaataaa agaatcaatc
     3361 caatagttaa atacactcga taattatata cgtctgtact tgaaataacc gaatacgaat
     3421 agctattttc aaagtcttca aaaatattag tggcataatc agtgtgaaat gcttgattga
     3481 atcgttccac aaatagtaaa atgaaccgtt cataggcttc tttttctctc gtcatcaaga
     3541 aaaattcacc ttcaaaatct tttattttga tcgattcttc aatggacgtt acgcgaaagg
     3601 aggtattaga atagggtacc ttctcgtgag aagatttgac atccccttga taatacaatg
     3661 tcatttcaag ctttggctgt atcttgaagt tccaagatgt atctacttca ttggattcat
     3721 ttactgtttt tttcatataa gaaagctcag tttctgatga gacattttca aataccatga
     3781 ttagctgctc atattcttct tgcccaataa aatgttgtgg caatcgaatc ggagtataag
     3841 tatctgtttg atggaaaaat tcatttgtcc gatagagtac ctgtttattt ataataacca
     3901 aggcaaaacc actcatgata ccggtcacta tgattgtgag taaaactgtg aacttttttt
     3961 tgattatcac cactctccct tacttaaatg attctttgat aaaatcatac cgtttttcat
     4021 caaaaaaacg tatctatcaa atgtataggt catgtagaat aaatctatat actgtagaat
     4081 cgaaaggaga agaagatgaa catcggagaa aaaatacgaa cttaccgcag aaattgtaag
     4141 cttacgcaaa aagaattagg cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg
     4201 gaaaattctc gtacaatgcc agaccttgaa atgctgtcac ttttacatca gactttaggt
     4261 ctgccgattg attttccttc tattgaatca gctaccgaat caatagaacc aacattgccg
     4321 gatgaacctc ttgttatcaa gaaatcttat tcatcaaaac aacccttctc actcaaaaag
     4381 acactatctt tctttctgct cgtcttttgt ttggtctatc tttttcttat caattatcaa
     4441 tataaaaatg aaacggttga tcgattcaat ccttttttga aaccacaaat cggctatacc
     4501 acactccctg taggatacgt ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt
     4561 gttcatggta catggctgtc ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct
     4621 gtaatcaaac acaaaggaaa acacgtcatg aacgttcgcc tgctcacttg ggaagccatt
     4681 ccctcagatt accaagaaat cctcatccaa gactatgacg atgccttaaa aagaagccct
     4741 caggagccag ataggcatta agtaggagat gaacgtagag atagaaaaaa tcaatgcaca
     4801 tgatcttatc aaaaaaaaac aggggtacaa attaattgaa aaagctttga gatgaaagtt
     4861 cctaaataaa aaagttcaga atgataggta aaaaaatgac taactattct agtattaggc
     4921 tatttgtatt attcttctgt tatttttata tcaaaaaatc cagaaataac taaatatagg
     4981 tatttctggc ttatagtata atatttacgc cacagccccc aatgattctt tttggaatca
     5041 tgatcaagga cggacataca caaaaatatt ttgcccattc gatccgaata gattaacgac
     5101 taatctagta agtaatatag attggtacgt agtcaaacca gcaaatggaa aagttgtcta
     5161 ttccacccca agggctttaa agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa
     5221 ggtacccatt aattctgggg gtacagttcc aatatcagca gcgcttatca taggtacaag
     5281 tacttttttc gctccggcat cttttgctac ttgtaaacag ttagctaaat tatctacctt
     5341 aatcatagaa ccaccaattg taatatcccc taaaataact aaacttggta atacaggttt
     5401 atttaaggaa actgagcata aagctattat agttgataac gataatgttt ttgtcatacc
     5461 aatgccattc atatcttgaa catgaacgac aaaatctttt gtcgtcaaac taatagactg
     5521 actaacactt ccataattag ctcttaaata tcggtatgcc gaatccatcg cttctcttgc
     5581 tccagcatct gaattgatcc cagtacgttc aaactttcca tttcccgaag tcatttgcgt
     5641 ttccaattta taggtaccta attgcccagt tttgccataa gataccgtgt acaaatgccc
     5701 tggcttccct aacccttctg gaatcaaagt tcctccacct tgttcaggaa cagaaacata
     5761 ctcttctcgc tcttcttcta aatcaatgta tgaaaaattg acatcataaa attccattcc
     5821 accaattttc tttaattgtt cttttacacg acgacgcatt tcaagagcaa atcttagtat
     5881 ttcttcgaca tcactttttt caaatctacc atcaggatac aacagtttca tcatcccaga
     5941 tacaatccga cgaactgcaa tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata
     6001 tttatcaaaa ctatcgccaa aactaacttt tcgaagctca cgcatgactt cagaaaaata
     6061 atctgtaatg aagccatagt catttgtaaa aaaatctggt cgatatttag gaatttccca
     6121 acctggtaaa taacaatgca tccgatccaa aaaggctgaa tccgtcccca taacctccgg
     6181 aaatggctca aataaattcg aagttttcaa tattacatca acgctttgat taatatttcc
     6241 tacaaaagcc attgatgctg aagcattctt ttcttctttg cctctagcaa aagatcctga
     6301 agccatgtaa tctttcatga tttgaacgcc atcattatcc ttaaatttaa tcccagcaac
     6361 ttcgtcaaaa gcaacacagt cccacatacc tacaagtccc attgtacgac tagccatatt
     6421 atagaaaagg tttgctactg ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat
     6481 ttctttatat acatgcgatt tacctgtacc acgtggtcct agttcacaga gattaaaatt
     6541 attttcaatt aatggaataa gtcttgctaa atgaagccat tttacccttt tattaaattg
     6601 tgtaggttcc ataccaatgg accgtagaag taagtcaatc cattcatttg tagtaaactc
     6661 cttgcgagcc atcttgtaag cctcaatgtc taaaccaggc atttggattg gatcaacctt
     6721 ttttgcaata aaagggctat tccaacgatc attctcatca taccaatatt ccatcgaaac
     6781 aatacaccag atccccccac ctaataatcg atcaaatttt ttcggatact cttcactaat
     6841 ttgaacattt tttaatccca ggttagagaa ttctgcttca taacaatctt tcttttcatt
     6901 taaatgtaca gttactttat caataactgt ataaccaccc aattcacgaa tttttgcttt
     6961 tactttttct gcttcatcag gacgcacata gtttcgagca agaatatttt taacattttc
     7021 cacacctttt tcaaccaatg tttcatccaa tgacgaagca tacataccaa gtaaatattc
     7081 caatacataa acaggtacat tcgcaccttc tttaattttc tttgttaaat cttttctgac
     7141 aatctttccg ggaaagaatt cagataactt taaaatttcc tgttgttcca ctagaattcc
     7201 tccatgtgtt cagtcattat aatagcaatt aaagtatatc atacttaacc agtaggcttg
     7261 ataatacaaa agaaaaagta tccacaaaaa attgtggata cctcgttttt atatatcaaa
     7321 atcaaatgat tctgcaaaag gattatctat aatgaatttt attcgctcgg tcaaatcatt
     7381 tttttgattt tttatcacta aataataagt atcttcaact gtatatcgac gatttacaaa
     7441 ttcaaaaaca aatttagtaa accgctgatt tgcatcttct gtttttgaat ccgcaacata
     7501 gatttcttca tttgagatac gttcaccata ttcatcttca aaataaagcc agaaagatgc
     7561 ggggtcataa acatcactaa ttgcattttt ttgatagaat tctaacgtgg ttgataatcc
     7621 tgtaattctg cggttgctag tcattaattc aattgctact ggttcctttg cattgctacc
     7681 tttggctacc gtaatttcta gaacagggac tagcatttct tgaatagatg accctccatg
     7741 aacgtagctt tgtcccccgc cagctttctt aaaaatactt gaagtaatcg ggacacaaat
     7801 gtttctagtg tcatcatttc ctaaagaatc tcccaaagag taatttttcg taccaattat
     7861 ttcgtattct tcttcactaa gaataaaacg acgttctaca cgatcttttt catttctaga
     7921 tggattttca atcttatcta cttcttcaac gtggcttctt ttataaataa atccatgatc
     7981 agccgtaact ataaaacgat agatactgcc atcattatgc aagcgattaa taagtcggga
     8041 aatttcgctt atcgctaaat cacaggcctc aaaaactcga ttttcattcg agtgatcacc
     8101 tgttctatcc acttgatcgt gataaatata aatgactgat ttgccattaa atacttctcg
     8161 tacctcagcc ttattcatct taaccacatc atcataattt aaagctacag catttgcatc
     8221 tcgttgctgg agaatcgcat ttcgattgga taaaccattc gtttttttcc catcaatcag
     8281 tacttctgtt ccttcattgt aagttagttg attgtttggt aataaggcgg ctttaccgaa
     8341 ttcagtaact gaaggtaata cagaaagaaa gctagccatg tttgttgaat acttcttttc
     8401 tctaactaac ttacgttcta aattcatccc taactcatat cgaaaagcat ctgaaataat
     8461 aactacagtt ttaacagagc gtttcattac agcttgatta taaaattttc gaatggatgg
     8521 acgattctta aattcaaaag tttgattcca aatttgtcct gattgatcaa gaaacttcga
     8581 ataatttgct tcaactaatc gatacaattg gccatatttt tgcttttctt ctatatgatc
     8641 actattccaa ataaatttcc gataatgcgt atcaacccaa tacatttcct tctcatattg
     8701 agttagcaaa ttatccatgt tcatcggcaa gcacaatctg tttttatcca caagataata
     8761 agcatgtttc aataattgat actcataaat ataatccgat gcgaaatgca ttttttcgcg
     8821 ctgctttaaa ctatcaaaaa ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt
     8881 taatcgttct gcataaaaca ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc
     8941 aattagatca tcaatttcta tactgtctaa taatccatca ccatcaatag cctgatatac
     9001 ttttttagat agctgttcaa agctttccat gtaacgtgta tcattcataa actgatccat
     9061 gaaagtaata atgtttgctt tcaaatgtag tatttcaaat cctttgaact tcttcggcat
     9121 ctcttgatca atttgataat aaaaatagtt caaataaaga cacatcgtca gcttttccaa
     9181 tgttggctgg ctatccgtaa agccaaattt catttgaata aattcccaaa atttagctgt
     9241 taactgatac ttatcaagtt cccttaaagg attctcccta ctttctgtaa agctttttag
     9301 aactacacgc agtaaatcat ttaatcgaat acttttcgat ttcaacaaaa cagccattat
     9361 cattaattca atcgtttctg atttattaat tgtcatatgc gtactaaatt caccaaaact
     9421 tctcatgcga tcctttgcat taaagaaatt acgatactgt ttaactgtct gatgaaatct
     9481 aagtggaata gccaattcat tcatgataat cgctaagcgg tctgcactaa attcctctga
     9541 ataattcaat acactcaaca aatgattatc ttcatcttta tcatcccaac gaggaaatgg
     9601 cgcatagatt aaatagttag tttcgggatc ttcatattcc aataaacgct tagttttaaa
     9661 ttgtgcgtcc ttttctaatc gatagatttt tgcattttga atgaccaatg agtcaatttc
     9721 ttcaagaaaa tcctgatttg aatcaaacca aaaaatgatt ttcctacatc cataattagg
     9781 aaaacgttga tttaattttt cttcaatttg ttgcgtattt atatctgtca tcaatctcac
     9841 ctaatcgttc tctttctata ccccttatat tgacataatt tggataattt cgcacgcaat
     9901 ttaagaataa aaaagaaagt tgttagaaaa caactttctt agataactat ttttttggtt
     9961 ctttgacccc ttttgcaaaa atttggaatt tctcgccggt tttaggatca cgctgcaact
    10021 tatcataatt gacaattaca ccatcatcta agtctaattc aattcgtttt aatgctaagt
    10081 gacccaactt ctcgtcatac tcacgaactt cttttaattg cttctgaatc tttacaattt
    10141 cttttgtcat tgcatttctt tcttttgggt tatcacttgt tgaaatttgt tgctgacgca
    10201 attcgattcg attgtcatac gctttttgta gttcgtgtag gtattcagta cgtacttttc
    10261 ctaattgatc tggtgtatag cgatgcaagt acattaacgc tttaaagcca ttttgttttc
    10321 ctgagtccaa ttgccaataa attggtcgat tggagtatgt ttttaaatgg tctttgtaaa
    10381 aatcttgtac gaagtaacga cgaataattt ctcgacttgt ccctttgcca cctaaagtgt
    10441 cagcgataaa ctggagattt tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat
    10501 caatgaaacg attgacaatg tcaatttggc tatccttgaa atactcatca tcggtgatga
    10561 gtaaaacatt atcagtatca ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc
    10621 cagcgaagat gagaccatct tcgtctaggg agtaacgacc gaagatacaa cctattgaat
    10681 aagaaataaa atccttaatt tccctcgcct tatcaggtaa ccttgcagag atatattcat
    10741 cttgaacctc ttttgttaat tctttttcaa gagaataatg attaatgaat atgtcgttca
    10801 gttcctcttc attcgatttt agagtattaa attgattaac caactgccta cttagacagt
    10861 taaatgcctc tgagagatct tttttaaaca tataacttaa caaaatgttt cttctaaact
    10921 cgtatgacgt ttcaagagca ttatagtctt ttctagctat atcgatattt tcatccactt
    10981 tttctcttat tttttttaat tttctctcag atgtaacttt aagaaaaggt agttttccca
    11041 ttggtccttc atggtaatcc aaggtgggag aaataaaagt taaaaagtga ttaactaatt
    11101 tagagtttac atagccaaat atttcttcaa agtcagttat atcctttgag aacagaacag
    11161 atcctttagt ttcaaaaatt gcctgattct gcaatctaaa agatacttgt ccagatgaaa
    11221 gtgtcgacca agtaattccc tttttaaaat agtaattaag attttgtggt ctagatttta
    11281 acttcccatt ttcattaaca taatttttta tttcatctcc atcattctcc cagttaacca
    11341 cgtattcatt gtttccatac cattttctaa aacgaccacc tttagttgta ggaaaccatt
    11401 tttttctaga ctttttagcg tcctctaatg ttttagcatc aaactttatt ttgttaaatt
    11461 ctacttcaaa ccaataccgc aaaaatttat tgttatttcc ggttgccaac ccttgttttg
    11521 catctgcaac atcgttagtt aattttcctt gagcaaaatc agtgattacg ttatcgcttg
    11581 cccagtaagc aatcggcatt cctgggattt ttttgaagtt gtcttgatta gtactataaa
    11641 aataatcgca ttcaccatgc tgatgaattg cttctaacgt tttaattttt tgaacttcca
    11701 ttccaccttt aaaatctgat aattttatat agcttccatc ttgttcattt gaatttttta
    11761 tcgtaaacgt acaaattgga acggttgctt cttcaaaagc tgaatactcc atttgaatca
    11821 aactagaaat gtttctttca ttaattaaaa atgagcgtaa cttttcatag ctagaaatga
    11881 acatccaaac aaatggcgtc ataaaggaag catatccatt atcaatcagc atttggctat
    11941 tcaatttaat aaacacagaa aatagatctt ttttgacatc agaatagtta tcatttacat
    12001 atttttttaa tttcttatcc attttattaa gataaggcgg attagtgact accgctacat
    12061 atttatcaac tagcatttca gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct
    12121 tcatcccata aagaatatcc atattcgtaa attcgttgaa ttttgatata tactctctta
    12181 cttttcctaa gtattcttta gttattgcct gattattttt atcgactaac ttaatgattg
    12241 atccaagttc tttcccattt tcaaattgat caaaaattaa ctctttgtat tcattccagc
    12301 gttcactata cttatcattg ttcaattcac taatacgctc ttgatactcg tctgtcactt
    12361 taggagaatc aataaactct cttaaattcg gcgatagact ttttttcaaa aaaagacgat
    12421 cgtattgacg ccctttcatc ataattgcaa aataagacaa ttgatacgct cttttatcga
    12481 tttcaagacc atataaattg tattcaacga ttgcacgtgc agcatcacgc tcagaatatc
    12541 ctaacgtaag ataaatttcc atcaatacat cgaaggcgta aactaaaata tgcccagacc
    12601 ccattgcgtt atcgataatt tttagttctt ctggttttaa attacttaca gatgttgctt
    12661 gttcaaaagc atatttatct ggaattaagt atttcaactt atttcttaat tcactattgg
    12721 gataatgatc taaccaatac tttcctaaac tattgtcaac catataacga acgacccaat
    12781 ctgtggtaaa tagttgagtt gctgccggaa tatcttgttt tttgacaatc cctttataaa
    12841 tattcaccac ttgattatgt ggctcactat tatagtactg atacaaccac ccaataatct
    12901 ctacctgtcc accagcttga acatagaaat catcctctgg aatacttaaa agatcagcga
    12961 ttacaccatc ttttttaata taagaagctg taaacaaaag ttctgtataa tcttctactt
    13021 cttcaaatag ctcaggtaag taatcattca attgattaca gatttttata aaaatcaatt
    13081 gatacagctt gtccattgct tctgctgaat tatctgtttt ccaagttctg acttgttgct
    13141 gttcattact agataattct tcaaagatat cggcatcata aatatctgta ataatatctg
    13201 gctcaatttt cccacttatt tcactagata atacacgata cgtgtcaggt aaataatcat
    13261 ttacctccat aaaccgaatg gcaatcaatc ggttaaacca cgtataagca acctcttcga
    13321 ttaactcttg aaaagcttct gaataagaag tgacagaagc acgttgtttt agttctgcaa
    13381 tcaatttgtt tctttgcgct atttcttttc cttgaattct tattcctgat ttttcgattt
    13441 cttgtaaatt gctatcaatt ttcttcacat cttcaatttt tgattcatca attcctaaac
    13501 gttttgcttt agtcattact tcatcaatta attttctacg ggcattgacc gcgaaattct
    13561 ttatcgcttt cttatccatt tttctttccc ttctatatgt aacctttgaa aagacaggca
    13621 cctttttatg cctgtctttt cactagaatt gaatcgtgat ttctgtattt tcttgaagtt
    13681 gttccttcag tttttgatta atttcagctg ttaactcatc aatatctgct gctgacttaa
    13741 tagttttaac atgaacgtta agtgttctca ttggtactgt tttctgttga atagtaggtt
    13801 ttactatcgc tatttcttca gcttttgctg cagagtctat ttcatctttt tcagtttcac
    13861 gaatcaagtt tgctgcttca atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt
    13921 cttcattatc gaaacgttga ttaaagatca aatactgttc tgctacgttt aatgataaga
    13981 aaacaagatc atctatagta ttcgcttgtt caatttttgc tttcagttct tgaaattctt
    14041 ggtcaactat tggtacaaat tgatccttaa aatcttttcc ttctaataat ttaaatgttg
    14101 cttgtttttc ttccgttaat ttatttaata tttcctgact ttccaactct aacagttgat
    14161 tatattgttc aataaattct tgaatcaact catttagttt catcatatct gaataaggat
    14221 cagccttttt caaaatggtt ttcatctcat gaatcaattg atcaatttct tggctagaag
    14281 tttgacgccg acttttttcg attaatctaa ttttatcttg tgcattctcc cagatttttt
    14341 tctgagtact attttgcttg tggaaatttt taattttatc atatgtttca gcccagtcga
    14401 ataattcatc gcgatatttt ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa
    14461 caattacttc aagatatttt tttatttgct catattcata agcactaggg taggtataag
    14521 tgttttgtaa atcaaaataa gagcgtaaat tgtctaattg accttttaaa tatttcttga
    14581 aattttccat tgtatgttca ttgttatttt catcaacaat attacgactt tgaaacagtt
    14641 ctctaccgat ttcgtcaacc aatttttttg cttttggcga aatcaccgcg cttggttcaa
    14701 tttttatctt ttcactttct cgtcgttttt gaataatcat tacaatacta tttggatcat
    14761 ccatccgtga aatttgtaca ttgttataaa gtaaatcaat tttaccagac atgaaaagtt
    14821 tagcgatgca ccattccaca tcaagttctg tgaatccata cggttgtttt gtttcaaaac
    14881 gatcacgaat tgttttcatc gtaatcgtta ggtgatttct cttttgatca ttgataaaat
    14941 tgaaaatttc ctgaactgct tgctcatttg atggatcttc aataatttca tttttgcttt
    15001 ctttcactaa atataagata tcttcttttt ctttaggcgc atcaatttgg tttaatttat
    15061 ggtatacttc ttcagccatt ttctgaagtg ctgattggaa ttttgcttta aagtcggagc
    15121 ctgtcagttg aatttctgca ccgttataga aaaattttgc agatttcaac agttcattta
    15181 attgatcgtt gatacttgca cttagttcct gtaattcacc agatttttcc gaacgaatcg
    15241 cgtcaaagtt ctttactaca ctcgaataag tcgttgttaa ataaagattc attctagcat
    15301 acattgctat atcatcttta aattctttat ctgatggcaa ttctacgaat aataagttac
    15361 gggaactctt aagcgcaagt tctgcctctt gacctgctaa atcagagtat ggtgaaataa
    15421 aattaactgt catttcagca tttccagctt gcttgtatgg acgttgatct aacacttgtg
    15481 cataagtaaa cgtataccgg tttttaaatt tatttccacc tgacatgcct aatttttcat
    15541 aggcaaaact atttcggtct agaacgccgt caaaaattcg tttagatagt tctacgatca
    15601 aatcactatc tgtaatattt tgatttttaa tctcacgagt aatttcctgt tcttcatcag
    15661 tttggaaaat ataactatct aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca
    15721 atgcttcttc tactcgtttt ttagtttcca ggcggtcttc gttgatattt cttatcatca
    15781 agctcgtaat attttcaaca tttggtttga tggcttgatc aacatattta atcatgaata
    15841 aaattcgtaa gacatttacg ttaaagttat ctatatcagt tggtctttga atatgagaat
    15901 tatttgtagc ttgtgtaatc actcggctaa ttgctgtatc aatccaacgt tctaacgagt
    15961 aataaaactg atctaaagaa accaaactgg ttgtatcatt ttccttattt cgttcagcgg
    16021 cttctttaaa cattgctagt aatgaacgtt caccagacga taagtttgat ccttgatagc
    16081 tgtgattacg aatctcggtt agaatttgcc ccaataaatt gaattgatag ggaataaatg
    16141 gataaacttc tgcgtaattt tcagcatctt ggaagagctt taattcagga ctatcaacaa
    16201 agagtaattt atttttcaat tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat
    16261 tggcaaactc ttttttcttc aaaatacgtt ctttaattac ttcatcggca tttgctgaag
    16321 ataaactcac agttgtggca aaacgatcct tgatcttaga aaaatcatta tcagaattgt
    16381 tatcaaagtt tgttacgtga tcaatcgctt cctgacttgt cactaatacc caagccttac
    16441 cttcacaaat tcgacccaaa tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat
    16501 taccgccgat atattgtccg acctcatcga ctagaaaggc aatatgatgg tctgcttctt
    16561 tggaatcaag atagctttta actagatttg caaagtcatc aatgcttatt aaataatttc
    16621 ctgatgcttt ttctaaaaaa ttatcaatag cggcttcact cataaaatcg attttaagtg
    16681 ccgcttcttt gatatcatct tggatccaat caaactcatg gcgctcttct tgccattttg
    16741 ccccattaat ctcttcaaaa gcagctttga atgcatcaaa cttttcttct ttaattaacc
    16801 gacgttcaaa atctgctaga tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct
    16861 ttaaaaaaac attcacgatt ccgtttttgt cttttttacc acctgcatca ctttttgaat
    16921 caatattaaa cagaataaca tctgtattta tttgtgagca ccgttccatc gttgccaaag
    16981 tcagtggatc tttgattttc ccatcttctt tgaagtaatc aatggctttc ttgtcattta
    17041 tttctcgatt tgcgagcaag taagaaataa tttttaagaa atgagattta cctgaaccaa
    17101 agaacccctg aatccaaaca cccattttat ctgttggtgt attgatactc cgttggtagg
    17161 catcaaaaat tgttttaaag tgtttttgta gttctttcgt aaccacgtat tcttctaatt
    17221 cttgttgaac attttcatcc tttgcttgac cgaccttaat aactccttga atatttcggt
    17281 caattggttt tgcaaaaata tctttaatta gcatttaatc tcctaccttc catcccaact
    17341 aaattctaaa tgctcgataa taattatcat ctttattggt attaagaatt gtcaaactta
    17401 attcatcata ttcccctgga tacatcatca caactggtac gcgatcaaat acctgatgca
    17461 ttttatttaa aatagagtgc gctctaatta atggatataa ttcgccaatt cctgtcagga
    17521 acacaactga tttttctggt gtatgggcat ccacatattc aaaaaataca ttttcttcct
    17581 caggtaaatc cattttcaat aaacgaccaa tgctttccac taatttttgt aaaccacgtc
    17641 gttcttccat cttaatcgct ttttcagtga aaccgcgttt ttctagatac tctaacataa
    17701 tcacatagag attataaact ttcacttcat
//
LOCUS       DANKAY010000054        16843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000054 DANKAY010000000
VERSION     DANKAY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            132..449
                     /locus_tag="PWY98_001764"
     CDS             132..449
                     /locus_tag="PWY98_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2037513.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(653..1018)
                     /locus_tag="PWY98_001765"
     CDS             complement(653..1018)
                     /locus_tag="PWY98_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037514.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1226..1708
                     /locus_tag="PWY98_001766"
     CDS             1226..1708
                     /locus_tag="PWY98_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037515.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1905..2795
                     /locus_tag="PWY98_001767"
     CDS             1905..2795
                     /locus_tag="PWY98_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2037516.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3120..5591
                     /locus_tag="PWY98_001768"
     CDS             3120..5591
                     /locus_tag="PWY98_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2037517.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5694..7430)
                     /locus_tag="PWY98_001769"
     CDS             complement(5694..7430)
                     /locus_tag="PWY98_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2037518.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7427..8131)
                     /locus_tag="PWY98_001770"
     CDS             complement(7427..8131)
                     /locus_tag="PWY98_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037519.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8262..8765
                     /locus_tag="PWY98_001771"
     CDS             8262..8765
                     /locus_tag="PWY98_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2037520.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8725..9063
                     /locus_tag="PWY98_001772"
     CDS             8725..9063
                     /locus_tag="PWY98_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2037521.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9084..10502
                     /gene="ffh"
                     /locus_tag="PWY98_001773"
     CDS             9084..10502
                     /gene="ffh"
                     /locus_tag="PWY98_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2037522.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10614..11192
                     /locus_tag="PWY98_001774"
     CDS             10614..11192
                     /locus_tag="PWY98_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2037523.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11196..11717
                     /locus_tag="PWY98_001775"
     CDS             11196..11717
                     /locus_tag="PWY98_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2037524.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11796..12350)
                     /locus_tag="PWY98_001776"
     CDS             complement(11796..12350)
                     /locus_tag="PWY98_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2037525.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12603..12878
                     /gene="rpsP"
                     /locus_tag="PWY98_001777"
     CDS             12603..12878
                     /gene="rpsP"
                     /locus_tag="PWY98_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2037526.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12890..13141
                     /locus_tag="PWY98_001778"
     CDS             12890..13141
                     /locus_tag="PWY98_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2037527.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13266..14057
                     /locus_tag="PWY98_001779"
     CDS             13266..14057
                     /locus_tag="PWY98_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037528.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14227..14748
                     /gene="rimM"
                     /locus_tag="PWY98_001780"
     CDS             14227..14748
                     /gene="rimM"
                     /locus_tag="PWY98_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2037529.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14738..15499
                     /gene="trmD"
                     /locus_tag="PWY98_001781"
     CDS             14738..15499
                     /gene="trmD"
                     /locus_tag="PWY98_001781"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2037530.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15635..15982
                     /gene="rplS"
                     /locus_tag="PWY98_001782"
     CDS             15635..15982
                     /gene="rplS"
                     /locus_tag="PWY98_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2037531.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(16114..>16843)
                     /locus_tag="PWY98_001783"
     CDS             complement(16114..>16843)
                     /locus_tag="PWY98_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2037532.1"
                     /translation="VSVIKSKLNLLFSYAMKKGYVEKNPIDEVVIDYKRESKTIKIKD
                     KFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDVHITNNNASVVI
                     NGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKG
                     TPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSD
                     ITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5601 a         2977 c         3393 g         4872 t
ORIGIN      
        1 gtaactatgc actgtaacga taatgctgct aaaatatctt tttacgaaaa acatcacaag
       61 gaaaaactta actatggtat tatctaaaca taattttttc ggtatttaat atttaattag
      121 gagggaatat tttgcttttt tatagtaatt ttatcttaat agtagcgata ttattacttc
      181 tgaatatatg gatttttgat aggagtagaa atgcaagtat aggttttaga actaaacgaa
      241 gtctgtcttc taaaaaaaat tgggtatact cacaaactat tttttatgga ggaattgttt
      301 taattagttt actttcttca acactttatt ctttgaatat tatagatgtt tccacctcaa
      361 attcaatatc tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt
      421 ttggagagaa gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatagtgat
      481 attaaaaatc agaaaagata gaaaaagcag gagatagata aaatgctgat atttcagcat
      541 tttttaagag tataaaagag aaaaaaagta ataaatgtat acatgatata agaaaaacat
      601 atatgtataa aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat
      661 gccgagcttt tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta
      721 agtaaatacc tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac
      781 gccattcagt aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa
      841 tgaataaaga tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa
      901 atatcaatgt gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat
      961 gcacataagg aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct
     1021 ttcatcacca cctaacaagt gttattattt tgttagtttt attgtaaatt gaatcctgca
     1081 ataatcaatt actttttcat tatttctaga ttaaatgaat aaactctcat gccattatct
     1141 ctaaaaaaaa agtttttccc cctaattttt ttagatagaa aattgtatag tatgatgaga
     1201 gaaaaagtag tttgggggaa cgttaatgag aagaaaagca gtaatcgatc ggctttattt
     1261 atttttttgg ttttttccta tcgttttgct accttttaca tgggtaaatg ctgttgtaca
     1321 aggtgcggag atattaggcc tgatccaagg aagcggattt gtggtttcta ggaattttcc
     1381 agctttgact gttttctatt tagtgataag tgtaggtctc ttattaccaa aatctctgat
     1441 caatcactac aattggcagc tgctttactt ttcttgctta ttagtattta catctatgtt
     1501 ttctttgctg cctcgcttga ttatcggccc taatggtttg agcgagagtg gattgaagga
     1561 ttcagcttat tcctccatgt atttcttgac gatcaaacca tggttctatt tagtaattgc
     1621 ctcatttatt cttgcaacat ttttttactt ctggacagaa atcaggataa aaagtgaacg
     1681 atctgtatct ataaaaaata actattgatg gtggatcatc agtaaaaaag catatgagct
     1741 gcgtaaaatg catgtttctc atatgctttt tttattatta tgtaaagaat gcgaaaaacg
     1801 tttacatgat ttttacataa cttaactata tttttacagc aaccatacga gggatttaca
     1861 gtcgactgat aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt
     1921 ttactctagg tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag
     1981 atggctctgc cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg
     2041 taggttcgac agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa
     2101 atccaaatta tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg
     2161 aagctggagc agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg
     2221 atgcttcaaa attagtggat cataaagtag cagtggtcgg gatggcgcca atcgtcaata
     2281 aagatacaga tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa
     2341 tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa
     2401 acggcagcgg tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc
     2461 agtcccaaga acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag
     2521 caatcagcta tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg
     2581 gtgtagaacc aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac
     2641 atatgtacac gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga
     2701 cagaagaaat ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg
     2761 aagtagaacg tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag
     2821 tcgttactat taaggaaaaa tcaactatgt ttcccacata tacatttttc ctttaattgt
     2881 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattaa
     2941 acaccgttta ttcgaaaaaa agagctacga catagaactg tcgtagctct ttttttgcta
     3001 tatgacagga atgaaaggtg tttgcaagct taatgtgagg aacactttcc atatctgttc
     3061 tatactggat aggagaagtt tggaaatatc cagaaaacga aatcaatcga ggtagccaca
     3121 tgaagaataa atggataaat cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata
     3181 aagtcatccg tctttttcca tcgtataaag aaggattaac ggcagatcaa gtcaaggaac
     3241 gaatagaaaa aggtgcagca aataattcag tcgatcccac attcaaaaca aatcagcaaa
     3301 tcgtattgga aaacatattt acgtatttca atttgatctt tcttatctta gccattttgc
     3361 tttgtttagt agaatcctat aagaacttga cttttttacc agttatcata gccaatacag
     3421 gaatcgccat ttttcaggaa attcgatcca aaaagatctt ggacgaacta aatgttttaa
     3481 atacagcaaa agtcaatgtc atacgggacg ggatagaaca aacaatagat atagaagacc
     3541 ttgtaataga tgacattgtt attttagaga ctggacatca aattccagca gatgcagagg
     3601 tcgtggaagg aaaactgcaa gttaatgagg ctttattgac aggcgaagca gatgaaataa
     3661 ccaaagaaat aggcgatcca ttaatgtccg gcagtttcat tgtttccgga aaagcttacg
     3721 cccgattaga caaagttgga gcagattcgt atatttccca gctgacagtc aaagccaaga
     3781 caatgggaga aggagaacag tcggagatga ttgcttctct taatcaattg atcaaatgga
     3841 ttgggatcat cattattcct atcggtatcc tgcttttttc tcaaagttac ttttttaatg
     3901 gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc attgatcggt atgatacctg
     3961 aaggactgta cttgctgacg acgattgcat tagcattaag tgcgactaaa ctagcaaagc
     4021 aaagagttct tttacacaat atgaaaagca ttgagacatt ggcaagggta aatgtccttt
     4081 gtgtagataa aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct
     4141 ctaaaaaaca agaaataatc agtcagtcag atcaattgga agcatgcatt gctgactatg
     4201 caaaagcgat ggcaggagac aatgcaacaa tggaagctgt taaagcatat tttacaaaaa
     4261 ccactgatca atcctatcac catgttattc ctttttcttc tgtccaaaaa ttcagcagtg
     4321 tagcgttggc agacaaggtg tatgttttag gagcgcccga aatggttttg cgcgatcaat
     4381 tgccggaata tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct
     4441 ttggtgtgta cgatgggatt cttgaagaac cagtattgac agaagcagtc acaccattag
     4501 gctatattct gattgctaat cctatcagaa aagaagcaag agccacattt gattacttca
     4561 aaaaacagca agtagccatc aaagtgattt ctggagataa tccgctcacc gtatccaatg
     4621 tagctgtcca agcagggatt tcacatgctg atcagtacgt tgatgcatct aaattatcag
     4681 aagaagaata tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc aagccagaac
     4741 aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac agtagctatg acaggtgatg
     4801 gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa
     4861 atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc
     4921 cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt
     4981 ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga
     5041 cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat
     5101 ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg
     5161 tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga
     5221 tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt
     5281 tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa
     5341 gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc
     5401 tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct
     5461 cggcagattc gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa
     5521 atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa atttctatcg atatttaaac
     5581 gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct
     5641 ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt
     5701 ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta
     5761 ccattaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa
     5821 tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca
     5881 atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag
     5941 cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat cagatttttg
     6001 ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga
     6061 atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa
     6121 ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg
     6181 atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa
     6241 gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt
     6301 gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt
     6361 tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct
     6421 gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc
     6481 tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc
     6541 tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc
     6601 tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca
     6661 atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtaca cgcccgatac
     6721 atcttactca tttgttcgct caacgcatta atcgtctgat acaattcttc ccattgatcg
     6781 ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc
     6841 agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga
     6901 acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt
     6961 aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg
     7021 actggtaatg cgacatataa aagatcctca ttcaaagtag tacttttgcg caaagcgcta
     7081 cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc
     7141 aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt
     7201 tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt
     7261 tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt
     7321 tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta
     7381 actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat cgttttggtt
     7441 cctgaaactt atagccaaat cctctaatag tcaccaagta ctttggatgt tttggatctc
     7501 gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga
     7561 agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt
     7621 tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta
     7681 cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct
     7741 cagcttcatc ttttgattgc ttacgaggtt caatccgtct gaatattgct ttcatacggg
     7801 ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc
     7861 caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt
     7921 tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta
     7981 aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg
     8041 cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag
     8101 gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct
     8161 gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat
     8221 aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat
     8281 gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg
     8341 aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa
     8401 ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag
     8461 caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca
     8521 aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc
     8581 cttcatgcgg aacccattcg atttatgacg gaagtttttc cggaataaaa aaaaatggtg
     8641 ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat
     8701 ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc
     8761 tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga attatattat
     8821 gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta
     8881 tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa actccatctt
     8941 ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat
     9001 aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa
     9061 taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg
     9121 cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc
     9181 gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta
     9241 ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac
     9301 aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag
     9361 agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag
     9421 gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc
     9481 cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag
     9541 gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg
     9601 tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg
     9661 caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga
     9721 cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg
     9781 tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg
     9841 gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat
     9901 tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta
     9961 gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag caagactacg
    10021 acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg
    10081 actttattga acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa
    10141 tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg
    10201 ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc
    10261 tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag
    10321 tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag
    10381 gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag
    10441 gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat
    10501 aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc
    10561 agcaattttt attctttact aatacaatta ctctaaacga agaggtggca cagatgagat
    10621 gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc
    10681 ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat
    10741 tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg
    10801 atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag
    10861 gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga
    10921 aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa
    10981 tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg
    11041 aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg
    11101 cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag
    11161 aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg
    11221 ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca
    11281 ctccaacagg taagcaaact acttatgaag ctttttatga aacaatcgat acgaccatca
    11341 tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag
    11401 agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg
    11461 taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct
    11521 gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat
    11581 ggtatatcca gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa
    11641 catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc
    11701 attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct
    11761 gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa
    11821 tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca
    11881 ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc
    11941 acattcaaaa agacaagtga ttggcgtaga tggtggtttg cgccaaagcc gccgatccca
    12001 cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt
    12061 cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga
    12121 gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca
    12181 ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca
    12241 aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg
    12301 taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc
    12361 ctccttcaaa ctatctataa taatcttaca aaagataagt aactggagca aataaaaaca
    12421 cttactgaca aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa
    12481 aatcttctat tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac
    12541 agggtaaaaa aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa
    12601 taatggcagt taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta
    12661 tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt
    12721 acaatccttt gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt
    12781 tatcaaaagg tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta
    12841 tgcaaaaaca ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt
    12901 aagcgactta gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt
    12961 agacgtcgta gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat
    13021 tggccgtatc attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg
    13081 tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta
    13141 aataaacgtg ctgtactcga attttcgagt gcagcttttt tgtttttttt attaattgaa
    13201 gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac
    13261 agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt
    13321 cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca
    13381 gtccagaaaa aagtagtacc aaaacggctt ccaccactga acaaacaatg gctacctcaa
    13441 ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc
    13501 ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag
    13561 gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga
    13621 atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa
    13681 caatccaagt tttgcagcca tttgagatcg acacaaatgg tcagcaagtg gacttaggtt
    13741 ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag
    13801 gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag
    13861 ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta
    13921 aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac
    13981 cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt
    14041 ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac
    14101 aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa
    14161 gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga
    14221 tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc
    14281 gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg
    14341 ctaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa
    14401 cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa
    14461 tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa
    14521 ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa
    14581 gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa
    14641 gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga
    14701 gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt
    14761 atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa
    14821 aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac
    14881 agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta
    14941 tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct
    15001 tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca
    15061 cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac
    15121 agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat
    15181 gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac
    15241 ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa
    15301 agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca
    15361 attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga
    15421 atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac
    15481 agcagatgaa gccaagtgac tttttctaaa atcttcttta cagtaggttg ataatatgtt
    15541 agtattttat ggtgagtgca aaagcactta actattacga tattccgctg tgagaaaagc
    15601 tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac
    15661 aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca
    15721 cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa
    15781 acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg
    15841 cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta
    15901 cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg
    15961 tatcaaagaa atccgtcgtt aatcatcgtt atcagacaga gaaagaaccc ttggtattca
    16021 agggttcttt ttttattatc tgttttttta tattaacgat ttctatttgg tacatttttg
    16081 attaataaaa aggggcaaaa aaggggcaac aagttacagt ttttctatat cttctttcaa
    16141 tttttctttt actccttttg tcacatgcaa ataaattttt tcagtgatat cactattttc
    16201 gtgaccaacg cgatcctgaa tggcatacag aggagttcct agttccgcta attttgaaat
    16261 atgggtatgt ctaaaaatat gtgaactaag ttttttatca atcttcatgt cagctttatg
    16321 acttctcaaa taggtgttaa ttgctgttag ttggaaagga gttcctttcg ttgtttgaaa
    16381 tagaaattga ccatttggat ttaattctag aagctcattg taaatagcta tcgctttttt
    16441 tggtaaatca atttctcgca ttccggcagc tgtttttgta gaatcagatt ttttcatatc
    16501 agctattgaa cgttctctat acatcatcgt cccatttata actactgatg cattattatt
    16561 ggtaatgtgt acatcatctt tacttaatgc gattgcttca ccagacctca tcccagtcaa
    16621 atatagccac tggaaaagga gagaatatct tttattgtgt gaagttgtga aatctactag
    16681 tctattatat tcatcatctt ctaaaaattt atctttaatt tttatcgttt ttgactctct
    16741 tttataatca ataactactt catcgatagg atttttttct acataacctt ttttcatagc
    16801 atatgaaaaa agaagattca atttggattt aattacactg acg
//
LOCUS       DANKAY010000055        16794 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000055 DANKAY010000000
VERSION     DANKAY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..384)
                     /locus_tag="PWY98_001784"
     CDS             complement(<1..384)
                     /locus_tag="PWY98_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037533.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="PWY98_001785"
     CDS             complement(569..967)
                     /locus_tag="PWY98_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2037534.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="PWY98_001786"
     CDS             complement(964..1737)
                     /locus_tag="PWY98_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2037535.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="PWY98_001787"
     CDS             complement(1734..2417)
                     /locus_tag="PWY98_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2037536.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="PWY98_001788"
     CDS             complement(2584..3471)
                     /locus_tag="PWY98_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2037537.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="PWY98_001789"
     CDS             complement(3468..4337)
                     /locus_tag="PWY98_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2037538.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="PWY98_001790"
     CDS             complement(4474..5760)
                     /locus_tag="PWY98_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2037539.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="PWY98_001791"
     CDS             6033..6611
                     /locus_tag="PWY98_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2037540.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="PWY98_001792"
     CDS             6611..7540
                     /locus_tag="PWY98_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037541.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="PWY98_001793"
     CDS             7537..9273
                     /locus_tag="PWY98_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2037542.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="PWY98_001794"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="PWY98_001794"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2037543.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="PWY98_001795"
     CDS             complement(10169..10678)
                     /locus_tag="PWY98_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2037544.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="PWY98_001796"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="PWY98_001796"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2037545.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="PWY98_001797"
     CDS             complement(11979..13070)
                     /locus_tag="PWY98_001797"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2037546.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="PWY98_001798"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="PWY98_001798"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2037547.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="PWY98_001799"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="PWY98_001799"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2037548.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="PWY98_001800"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="PWY98_001800"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2037549.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4856 a         3708 c         2969 g         5261 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctga
//
LOCUS       DANKAY010000056        16615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000056 DANKAY010000000
VERSION     DANKAY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="PWY98_001801"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="PWY98_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2037550.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="PWY98_001802"
     CDS             complement(680..1666)
                     /locus_tag="PWY98_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2037551.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="PWY98_001803"
     CDS             complement(1689..2255)
                     /locus_tag="PWY98_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2037552.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="PWY98_001804"
     CDS             complement(2269..2685)
                     /locus_tag="PWY98_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2037553.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="PWY98_001805"
     CDS             complement(3142..5076)
                     /locus_tag="PWY98_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2037554.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5357..6313
                     /locus_tag="PWY98_001806"
     CDS             5357..6313
                     /locus_tag="PWY98_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2037555.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="PWY98_001807"
     CDS             6403..7506
                     /locus_tag="PWY98_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2037556.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="PWY98_001808"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="PWY98_001808"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2037557.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="PWY98_001809"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="PWY98_001809"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2037558.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12140)
                     /locus_tag="PWY98_001810"
     CDS             complement(10887..12140)
                     /locus_tag="PWY98_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2037559.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="PWY98_001811"
     CDS             complement(12146..13069)
                     /locus_tag="PWY98_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2037560.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="PWY98_001812"
     CDS             complement(13290..15782)
                     /locus_tag="PWY98_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2037561.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="PWY98_001813"
     CDS             complement(15789..16256)
                     /locus_tag="PWY98_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2037562.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="PWY98_001814"
     tRNA            complement(16392..16465)
                     /locus_tag="PWY98_001814"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="PWY98_001815"
     tRNA            complement(16523..16596)
                     /locus_tag="PWY98_001815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4906 a         3477 c         2957 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DANKAY010000057        16016 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000057 DANKAY010000000
VERSION     DANKAY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            226..894
                     /locus_tag="PWY98_001816"
     CDS             226..894
                     /locus_tag="PWY98_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2037563.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="PWY98_001817"
     CDS             complement(1094..1324)
                     /locus_tag="PWY98_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037564.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="PWY98_001818"
     CDS             complement(1451..2833)
                     /locus_tag="PWY98_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2037565.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="PWY98_001819"
     CDS             complement(2989..4176)
                     /locus_tag="PWY98_001819"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2037566.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="PWY98_001820"
     CDS             complement(4183..5214)
                     /locus_tag="PWY98_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2037567.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="PWY98_001821"
     CDS             complement(5230..6684)
                     /locus_tag="PWY98_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2037568.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="PWY98_001822"
     CDS             6859..7764
                     /locus_tag="PWY98_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2037569.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="PWY98_001823"
     CDS             7855..8532
                     /locus_tag="PWY98_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2037570.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="PWY98_001824"
     CDS             8529..8699
                     /locus_tag="PWY98_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037571.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="PWY98_001825"
     CDS             8813..9709
                     /locus_tag="PWY98_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2037572.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="PWY98_001826"
     CDS             complement(9764..11134)
                     /locus_tag="PWY98_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2037573.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="PWY98_001827"
     CDS             11372..12118
                     /locus_tag="PWY98_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2037574.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="PWY98_001828"
     CDS             12115..12954
                     /locus_tag="PWY98_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2037575.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="PWY98_001829"
     CDS             12955..13905
                     /locus_tag="PWY98_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2037576.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="PWY98_001830"
     CDS             14046..14798
                     /locus_tag="PWY98_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037577.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="PWY98_001831"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="PWY98_001831"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2037578.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5195 a         3000 c         3226 g         4595 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaac
//
LOCUS       DANKAY010000058        15742 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000058 DANKAY010000000
VERSION     DANKAY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(24..722)
                     /locus_tag="PWY98_001832"
     CDS             complement(24..722)
                     /locus_tag="PWY98_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2037579.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="PWY98_001833"
     CDS             complement(715..2067)
                     /locus_tag="PWY98_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2037580.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="PWY98_001834"
     CDS             complement(2290..3690)
                     /locus_tag="PWY98_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2037581.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="PWY98_001835"
     CDS             3851..4588
                     /locus_tag="PWY98_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2037582.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="PWY98_001836"
     CDS             4713..5192
                     /locus_tag="PWY98_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2037583.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="PWY98_001837"
     CDS             5189..5956
                     /locus_tag="PWY98_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037584.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="PWY98_001838"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="PWY98_001838"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2037585.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="PWY98_001839"
     CDS             complement(6859..7461)
                     /locus_tag="PWY98_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2037586.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="PWY98_001840"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="PWY98_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="PWY98_001841"
     CDS             8142..8726
                     /locus_tag="PWY98_001841"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2037587.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="PWY98_001842"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="PWY98_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2037588.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="PWY98_001843"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="PWY98_001843"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2037589.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="PWY98_001844"
     CDS             10627..10806
                     /locus_tag="PWY98_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037590.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="PWY98_001845"
     CDS             10828..11853
                     /locus_tag="PWY98_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2037591.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="PWY98_001846"
     CDS             12028..13272
                     /locus_tag="PWY98_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2037592.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="PWY98_001847"
     CDS             13425..14372
                     /locus_tag="PWY98_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2037593.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="PWY98_001848"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="PWY98_001848"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2037594.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="PWY98_001849"
     CDS             complement(15274..15516)
                     /locus_tag="PWY98_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037595.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="PWY98_001850"
     tRNA            15637..15709
                     /locus_tag="PWY98_001850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DANKAY010000059        15646 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000059 DANKAY010000000
VERSION     DANKAY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(133..2478)
                     /locus_tag="PWY98_001851"
     CDS             complement(133..2478)
                     /locus_tag="PWY98_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2037596.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="PWY98_001852"
     CDS             2757..3659
                     /locus_tag="PWY98_001852"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2037597.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="PWY98_001853"
     CDS             3870..5645
                     /locus_tag="PWY98_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2037598.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="PWY98_001854"
     CDS             complement(5711..7297)
                     /locus_tag="PWY98_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2037599.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="PWY98_001855"
     CDS             7548..7772
                     /locus_tag="PWY98_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2037600.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="PWY98_001856"
     CDS             7914..9665
                     /locus_tag="PWY98_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2037601.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="PWY98_001857"
     CDS             9665..11449
                     /locus_tag="PWY98_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037602.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /locus_tag="PWY98_001858"
                     /pseudo
     CDS             11784..12636
                     /locus_tag="PWY98_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12733..14619
                     /locus_tag="PWY98_001859"
     CDS             12733..14619
                     /locus_tag="PWY98_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2037603.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="PWY98_001860"
     CDS             complement(14660..15562)
                     /locus_tag="PWY98_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2037604.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         4804 a         2861 c         3238 g         4743 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atcttatata gcttaa
//
LOCUS       DANKAY010000060        15558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000060 DANKAY010000000
VERSION     DANKAY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            168..2228
                     /locus_tag="PWY98_001861"
     CDS             168..2228
                     /locus_tag="PWY98_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2037605.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2311..2880
                     /locus_tag="PWY98_001862"
     CDS             2311..2880
                     /locus_tag="PWY98_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037606.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3055..3861)
                     /locus_tag="PWY98_001863"
     CDS             complement(3055..3861)
                     /locus_tag="PWY98_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037607.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3999..5513
                     /locus_tag="PWY98_001864"
     CDS             3999..5513
                     /locus_tag="PWY98_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2037608.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5916..6899)
                     /locus_tag="PWY98_001865"
     CDS             complement(5916..6899)
                     /locus_tag="PWY98_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2037609.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6904..7551)
                     /locus_tag="PWY98_001866"
     CDS             complement(6904..7551)
                     /locus_tag="PWY98_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2037610.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7636..8268)
                     /locus_tag="PWY98_001867"
     CDS             complement(7636..8268)
                     /locus_tag="PWY98_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2037611.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="PWY98_001868"
     CDS             complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="PWY98_001868"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2037612.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8832..9569)
                     /locus_tag="PWY98_001869"
     CDS             complement(8832..9569)
                     /locus_tag="PWY98_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2037613.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9566..10555)
                     /locus_tag="PWY98_001870"
     CDS             complement(9566..10555)
                     /locus_tag="PWY98_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2037614.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10813..11355
                     /locus_tag="PWY98_001871"
     CDS             10813..11355
                     /locus_tag="PWY98_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037615.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11372..12457
                     /locus_tag="PWY98_001872"
     CDS             11372..12457
                     /locus_tag="PWY98_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037616.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12469..13275
                     /locus_tag="PWY98_001873"
     CDS             12469..13275
                     /locus_tag="PWY98_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037617.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13276..14103
                     /locus_tag="PWY98_001874"
     CDS             13276..14103
                     /locus_tag="PWY98_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037618.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14100..15173
                     /locus_tag="PWY98_001875"
     CDS             14100..15173
                     /locus_tag="PWY98_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2037619.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         5022 a         2798 c         3152 g         4586 t
ORIGIN      
        1 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
       61 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
      121 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
      181 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
      241 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
      301 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
      361 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
      421 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
      481 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
      541 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
      601 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
      661 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
      721 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
      781 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
      841 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
      901 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
      961 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     1021 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     1081 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     1141 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     1201 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     1261 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     1321 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     1381 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     1441 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     1501 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     1561 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     1621 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     1681 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     1741 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     1801 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     1861 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     1921 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     1981 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     2041 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     2101 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     2161 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     2221 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     2281 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     2341 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     2401 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     2461 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     2521 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     2581 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     2641 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     2701 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     2761 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     2821 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     2881 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     2941 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     3001 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     3061 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     3121 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     3181 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     3241 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     3301 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     3361 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     3421 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     3481 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     3541 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     3601 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     3661 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     3721 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     3781 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     3841 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     3901 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     3961 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     4021 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     4081 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     4141 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     4201 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     4261 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     4321 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     4381 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     4441 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     4501 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     4561 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     4621 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     4681 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     4741 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     4801 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     4861 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     4921 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     4981 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     5041 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     5101 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     5161 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     5221 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     5281 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     5341 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     5401 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     5461 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     5521 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     5581 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     5641 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     5701 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     5761 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     5821 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     5881 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     5941 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     6001 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     6061 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     6121 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     6181 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     6241 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     6301 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     6361 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     6421 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     6481 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     6541 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     6601 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     6661 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     6721 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     6781 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     6841 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     6901 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     6961 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     7021 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     7081 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     7141 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     7201 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     7261 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     7321 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     7381 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     7441 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     7501 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     7561 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     7621 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     7681 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     7741 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     7801 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     7861 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     7921 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     7981 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     8041 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     8101 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
     8161 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
     8221 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
     8281 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
     8341 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
     8401 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
     8461 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
     8521 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
     8581 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
     8641 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
     8701 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
     8761 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
     8821 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
     8881 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
     8941 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
     9001 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
     9061 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
     9121 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
     9181 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
     9241 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
     9301 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
     9361 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
     9421 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
     9481 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
     9541 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
     9601 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
     9661 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
     9721 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
     9781 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
     9841 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
     9901 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
     9961 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    10021 aggtaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    10081 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    10141 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    10201 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    10261 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    10321 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    10381 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    10441 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    10501 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg
    10561 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    10621 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    10681 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc tttttcaata
    10741 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    10801 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    10861 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    10921 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    10981 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    11041 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    11101 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    11161 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    11221 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    11281 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    11341 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    11401 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    11461 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    11521 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    11581 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    11641 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    11701 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    11761 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    11821 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    11881 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    11941 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    12001 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    12061 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    12121 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    12181 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    12241 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    12301 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    12361 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    12421 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    12481 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    12541 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    12601 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    12661 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    12721 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    12781 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    12841 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    12901 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    12961 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    13021 cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    13081 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    13141 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    13201 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    13261 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    13321 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    13381 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    13441 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    13501 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    13561 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    13621 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    13681 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    13741 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    13801 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    13861 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    13921 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    13981 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    14041 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    14101 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    14161 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    14221 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    14281 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    14341 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    14401 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    14461 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    14521 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    14581 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    14641 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagctgacag
    14701 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    14761 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    14821 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    14881 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    14941 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    15001 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    15061 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    15121 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    15181 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    15241 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    15301 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    15361 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    15421 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    15481 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    15541 taaattattt tgtcagat
//
LOCUS       DANKAY010000061        15255 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000061 DANKAY010000000
VERSION     DANKAY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            248..1021
                     /locus_tag="PWY98_001876"
     CDS             248..1021
                     /locus_tag="PWY98_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2037620.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="PWY98_001877"
     CDS             1018..1785
                     /locus_tag="PWY98_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2037621.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="PWY98_001878"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="PWY98_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2037622.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="PWY98_001879"
     CDS             3750..4070
                     /locus_tag="PWY98_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2037623.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="PWY98_001880"
     CDS             4070..4420
                     /locus_tag="PWY98_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2037624.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="PWY98_001881"
     CDS             4587..5525
                     /locus_tag="PWY98_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2037625.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="PWY98_001882"
     CDS             5540..6370
                     /locus_tag="PWY98_001882"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2037626.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="PWY98_001883"
     CDS             6414..7439
                     /locus_tag="PWY98_001883"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2037627.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="PWY98_001884"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="PWY98_001884"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2037628.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="PWY98_001885"
     CDS             8517..8957
                     /locus_tag="PWY98_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2037629.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="PWY98_001886"
     CDS             8960..9562
                     /locus_tag="PWY98_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2037630.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="PWY98_001887"
     CDS             complement(9625..10728)
                     /locus_tag="PWY98_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2037631.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="PWY98_001888"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="PWY98_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2037632.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="PWY98_001889"
     CDS             12294..12689
                     /locus_tag="PWY98_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037633.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="PWY98_001890"
     CDS             12689..12940
                     /locus_tag="PWY98_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037634.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="PWY98_001891"
     CDS             12983..13378
                     /locus_tag="PWY98_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037635.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="PWY98_001892"
     CDS             13375..14631
                     /locus_tag="PWY98_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HDL2037636.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="PWY98_001893"
     CDS             14643..14966
                     /locus_tag="PWY98_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037637.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="PWY98_001894"
     CDS             14959..15225
                     /locus_tag="PWY98_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037638.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DANKAY010000062        14590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000062 DANKAY010000000
VERSION     DANKAY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(157..723)
                     /locus_tag="PWY98_001895"
     CDS             complement(157..723)
                     /locus_tag="PWY98_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2037639.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(723..1679)
                     /locus_tag="PWY98_001896"
     CDS             complement(723..1679)
                     /locus_tag="PWY98_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2037640.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1679..2335)
                     /locus_tag="PWY98_001897"
     CDS             complement(1679..2335)
                     /locus_tag="PWY98_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2037641.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2705..4723
                     /locus_tag="PWY98_001898"
     CDS             2705..4723
                     /locus_tag="PWY98_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2037642.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4793..4999
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5150..7564
                     /locus_tag="PWY98_001899"
     CDS             5150..7564
                     /locus_tag="PWY98_001899"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2037643.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7912..9465
                     /locus_tag="PWY98_001900"
     CDS             7912..9465
                     /locus_tag="PWY98_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2037644.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9846..11630)
                     /locus_tag="PWY98_001901"
     CDS             complement(9846..11630)
                     /locus_tag="PWY98_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037645.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11645..13366)
                     /locus_tag="PWY98_001902"
     CDS             complement(11645..13366)
                     /locus_tag="PWY98_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037646.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13466..14227
                     /locus_tag="PWY98_001903"
     CDS             13466..14227
                     /locus_tag="PWY98_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2037647.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4734 a         2697 c         2908 g         4251 t
ORIGIN      
        1 caaaaacagc ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt
       61 gagcaccgtg aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa
      121 acgcaaaaca tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata
      181 taaaataggc ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc
      241 ctgtggcagt ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg
      301 accatagtag acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc
      361 catagctgtg cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata
      421 ccctagattg aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt
      481 ttcgtggcct tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc
      541 acgagtagca tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc
      601 gatgtgttct gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg
      661 atcccctggt tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa
      721 catcagacgc tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga
      781 agttcttcat caattgcatt caagacttcc cattttttca agctccacat tggtccaatc
      841 aaggaatccc acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt
      901 tccagctggt cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct
      961 tcatgatagt cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt
     1021 ccttggatat ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt
     1081 tcgcctggta atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta
     1141 gccacgccat ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct
     1201 tcaaaagttg tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca
     1261 aggtaatcaa ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg
     1321 acacctggta aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg
     1381 gtgtttgtaa aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc
     1441 atcatgtcga tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg
     1501 tctcccgaac cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga
     1561 ttcggacagt caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt
     1621 cgtaaagcgt aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt
     1681 aaaagtcctt ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat
     1741 aagtcaggca cagccaagaa agactgagta agtaaccgcc gatgatatca gtaggaaaat
     1801 gaacgcccag ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt
     1861 gaagcaatgt gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac
     1921 cgtaaagaat catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta
     1981 ctaagtgcgc caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga
     2041 ttccagcaat tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat
     2101 accacatcca taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt
     2161 tagtcataaa gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa
     2221 accactgatc gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga
     2281 aaaacaggac aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat
     2341 tcctcctttg aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag
     2401 agataaaacc aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact
     2461 gtaatcggaa agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt
     2521 ttttcgaaat ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag
     2581 ttgttcatta cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt
     2641 ttaataaata ttaagagaag ttataattat taataataga taaattgaag ttggaggata
     2701 gaagatggaa aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg
     2761 aaaagcaaaa cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc
     2821 cccattagta cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag
     2881 aatcaataca gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc
     2941 caacgatcta tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag
     3001 tagtgcgtta tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg
     3061 acaaaccgtc tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga
     3121 gcctttccga caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag
     3181 aaatacttca tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc
     3241 ctatacagat gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg
     3301 caaattaaat aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg
     3361 aaacgcaggc ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg
     3421 cggttcaagc aattctggta atacaaactc aggttcagca acttcgacca cttatactgt
     3481 ccaatctggt gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt
     3541 gatccaatgg aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa
     3601 aggtagccag gaaaatggct cttcgacgaa taattccggt aacaatacca attcttcagg
     3661 aaatgctggt acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg
     3721 tgattctgtt tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg
     3781 gaacaatatc aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg
     3841 ttcttctaat gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc
     3901 gtccagctca agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga
     3961 ctctgtttgg agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa
     4021 caatatccaa aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc
     4081 aacaaacagc acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt
     4141 taatactggc agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg
     4201 gagcgtggca aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa
     4261 aaacaacttc atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac
     4321 aaatacaggt tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac
     4381 aagtacatca tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag
     4441 cgtagcgaac aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa
     4501 taatttcatc tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa
     4561 ccaacaatca gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag
     4621 tgttgctgac agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa
     4681 caactttatt tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa
     4741 ataacgattg aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac
     4801 ttgaaaagga agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg
     4861 cacggatgct tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg
     4921 gcggtgacgg ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg
     4981 cgtagattac gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg
     5041 aagtttgaga aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt
     5101 tatatttata atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa
     5161 tcataaagaa attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac
     5221 ccatgatgat cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg
     5281 tcaagggcta catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt
     5341 gaaaagaagt caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc
     5401 tgctgaacaa tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat
     5461 cgaaacattc cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt
     5521 gaatacaact gatccggagt attataaatg gacacaatgg atctttacga agctttatga
     5581 aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt
     5641 tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag
     5701 aaaaccaatg cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga
     5761 tctagaatta gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg
     5821 ttcagtagga gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt
     5881 tacgacacga ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga
     5941 tcttgttaga gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga
     6001 gacagctaaa aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt
     6061 tactggtgct tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga
     6121 ttatgtatta tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg
     6181 tgactttgaa ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga
     6241 tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg
     6301 catgaacaaa caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg
     6361 gaaaaaagaa gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg
     6421 tgaaccaatc ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga
     6481 gctaccatta cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc
     6541 attagcaaat atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg
     6601 tcgcgaaacg aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat
     6661 cgaccctcat aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt
     6721 tgacatctac attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg
     6781 gcataaattc ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata
     6841 caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt
     6901 agtcaatcct gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat
     6961 gttcatgggg ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg
     7021 taaattcttg gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg
     7081 tatcactaca ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt
     7141 gacagaagac tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat
     7201 caatgaagcc tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact
     7261 actcgcaccg attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg
     7321 tggtatttct tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt
     7381 agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt
     7441 aggaaaagat gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga
     7501 aggcaaaaca gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa
     7561 ttaattggct ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa
     7621 tgaacttcat tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa
     7681 aggttttgat tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt
     7741 aagagatctc tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa
     7801 actacctgaa attgagagta cataccaaat tcataaattt tttattagtg ggactagtga
     7861 ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa
     7921 gaactattta ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa
     7981 agagcgaaaa gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt
     8041 ttcggagctg tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg
     8101 atgcaaaaat taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt
     8161 gtgcttattg tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc
     8221 gatcaatggc ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat
     8281 gatgactatc tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt
     8341 gattgccagt tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa
     8401 tcaaaattcg gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa
     8461 aaaagccagt cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt
     8521 tttttcctca ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg
     8581 tcttctcata taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata
     8641 caaaagacaa atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa
     8701 aagataaatg atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt
     8761 ggtgctaatt gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc
     8821 ctgacaacgg ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc
     8881 gatttctcat ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat
     8941 aatggtaaaa catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga
     9001 aaagcggtat tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct
     9061 gcagaaactt tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga
     9121 agtacaatga tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt
     9181 gtttcgacac gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca
     9241 gttctgtcga ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat
     9301 gaagttactc aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga
     9361 ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca
     9421 tggcaattcg ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg
     9481 agcctgacgt tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg
     9541 ttgaatagtg taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta
     9601 tagcttctcg tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg
     9661 tttattcgta aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa
     9721 ggagctatcc aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta
     9781 catttaaaaa agctatcgat catggactaa tcatacatga tcgatagctt tttagataat
     9841 gtttgttacg ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg
     9901 agtagttcag aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg
     9961 tctgcatttt caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc
    10021 acagcttcca ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct
    10081 tcatctaaaa taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt
    10141 tgctgtcctt gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc
    10201 agtgttcgaa taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt
    10261 gtggcatcca ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta
    10321 ttttgtaaaa ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca
    10381 agaccgtcga attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact
    10441 aaagtcgttt ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact
    10501 gaaaaatcga cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg
    10561 aaagctattt ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca
    10621 tctgtttcat ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag
    10681 ttgatcacat aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa
    10741 gcttgtaaga agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca
    10801 cttatgatga aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata
    10861 aattgagcac gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct
    10921 gcatgagctt gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt
    10981 cgattattta aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc
    11041 gcaatcttag ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc
    11101 ttgctttcga tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa
    11161 aattgattga tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga
    11221 ctaatgatgt cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt
    11281 tttgtgattt cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt
    11341 tcctgaataa atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg
    11401 aacaacaatg cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt
    11461 tctgataaga gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg
    11521 ttcccgaata aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt
    11581 ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt
    11641 ctttctatat tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt
    11701 cttcatgagt accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt
    11761 gttggattgt actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat
    11821 gaagggctgc tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga
    11881 atacatagat ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc
    11941 cccctgaata attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt
    12001 ccactagttc ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat
    12061 attcatttcc catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag
    12121 ggacataact gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac
    12181 caagggttac ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac
    12241 tttttccaga tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat
    12301 caatatcttc tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag
    12361 cagctgcatt tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa
    12421 tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga
    12481 taatgaccaa taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga
    12541 ctacagcagt cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc
    12601 acaaaataga agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa
    12661 atattcggtt cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct
    12721 tttcttcata tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc
    12781 tcaatgtgat ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc
    12841 gtttcatcag tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg
    12901 ttagtaatag agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg
    12961 gtaagatcat ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca
    13021 ttcttgtcaa aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga
    13081 atttacggaa atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa
    13141 agtaactaga ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc
    13201 caattttttt tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta
    13261 ctaattttgg tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga
    13321 gagcgagtcc tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt
    13381 tttcctgata cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa
    13441 taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga
    13501 actatttgat ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc
    13561 aagtagacaa gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta
    13621 tgatatcgtt ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg
    13681 aaaaacattg acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga
    13741 actggtggtg atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt
    13801 gatgatcgat acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat
    13861 ctcaacggag tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt
    13921 tgggatgatg agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc
    13981 tagtgaagta catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta
    14041 tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat
    14101 cagagaaaga atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt
    14161 gacgaatacg gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa
    14221 aaaataggaa atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa
    14281 aaattttctg gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa
    14341 ttttagtttt ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata
    14401 tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt
    14461 ataattcatg gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt
    14521 gtatcagatg agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt
    14581 gttgcttaca
//
LOCUS       DANKAY010000063        14484 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000063 DANKAY010000000
VERSION     DANKAY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            248..781
                     /locus_tag="PWY98_001904"
     CDS             248..781
                     /locus_tag="PWY98_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2037648.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1271..1795)
                     /locus_tag="PWY98_001905"
     CDS             complement(1271..1795)
                     /locus_tag="PWY98_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037649.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1788..2261)
                     /gene="tsaE"
                     /locus_tag="PWY98_001906"
     CDS             complement(1788..2261)
                     /gene="tsaE"
                     /locus_tag="PWY98_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2037650.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2384..3364)
                     /gene="pta"
                     /locus_tag="PWY98_001907"
     CDS             complement(2384..3364)
                     /gene="pta"
                     /locus_tag="PWY98_001907"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2037651.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3517..4197)
                     /locus_tag="PWY98_001908"
     CDS             complement(3517..4197)
                     /locus_tag="PWY98_001908"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2037652.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4323..5192
                     /locus_tag="PWY98_001909"
     CDS             4323..5192
                     /locus_tag="PWY98_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2037653.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5218..5733
                     /locus_tag="PWY98_001910"
     CDS             5218..5733
                     /locus_tag="PWY98_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037654.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5780..6373)
                     /locus_tag="PWY98_001911"
     CDS             complement(5780..6373)
                     /locus_tag="PWY98_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037655.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6398..6577)
                     /locus_tag="PWY98_001912"
     CDS             complement(6398..6577)
                     /locus_tag="PWY98_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037656.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6654..7163)
                     /locus_tag="PWY98_001913"
     CDS             complement(6654..7163)
                     /locus_tag="PWY98_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037657.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7259..7639)
                     /locus_tag="PWY98_001914"
     CDS             complement(7259..7639)
                     /locus_tag="PWY98_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2037658.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            7969..8718
                     /locus_tag="PWY98_001915"
     CDS             7969..8718
                     /locus_tag="PWY98_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2037659.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9039..10256)
                     /locus_tag="PWY98_001916"
     CDS             complement(9039..10256)
                     /locus_tag="PWY98_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2037660.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(10514..11791)
                     /locus_tag="PWY98_001917"
     CDS             complement(10514..11791)
                     /locus_tag="PWY98_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037661.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(11870..12514)
                     /gene="pcp"
                     /locus_tag="PWY98_001918"
     CDS             complement(11870..12514)
                     /gene="pcp"
                     /locus_tag="PWY98_001918"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2037662.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(12713..13657)
                     /locus_tag="PWY98_001919"
     CDS             complement(12713..13657)
                     /locus_tag="PWY98_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2037663.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(13659..14345)
                     /locus_tag="PWY98_001920"
     CDS             complement(13659..14345)
                     /locus_tag="PWY98_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2037664.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4161 a         2995 c         2612 g         4716 t
ORIGIN      
        1 gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac
       61 aactttgcat aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca
      121 caaggaatga gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt
      181 ttttgttaaa tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt
      241 tacagaaatg aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc
      301 ttgttctctt gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt
      361 gatacagcct gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga
      421 agacgtacgc aatgcgccga aattcccaga agtctggcaa actatccaac catattttca
      481 aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg
      541 cttagattat tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag
      601 ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa
      661 catccaatta gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct
      721 gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta
      781 aaagaatact tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca
      841 aggagcaatt tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg
      901 agctggcagg agaacaccgt ttattggaga ggttgtgaaa aagctgttct gcatcaagta
      961 ataagaacag ccaaacaaaa atagcttctc atatttttca tttggttgga cttattaccg
     1021 cagatgcaag cttttggaca ccgtttacca agaggttgtg aaatcagcgt tttgacctga
     1081 gtattagaga aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac
     1141 cgaaggtcag ctgattgaac accgtttatt caaaaaaaat ggactgcgac acgacttttg
     1201 tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga tgacagtttc tcactctttt
     1261 cctatttctt ctattcaatc aaataactca ttttatatac atctaaaaat tcacctagat
     1321 ctgttcttgc ccctctaggc atgaccgctt cgatttggaa gcccattttt cggtaaaggt
     1381 gtacggctct ttcattacga acttgaacgt caagctccaa tcgaaaaagt acacccattt
     1441 ccttcgccca actgatgatt tcttctagca taagagtgcc tagtcccatt ccccaatatt
     1501 cctgcagaat actgataccg acttctccca cgtgagacaa acgaaattga ctgtctgcct
     1561 tgacagatgc tgttccaata atcgtccctt catagattgc aagcagcaat aaattattat
     1621 tgctttcgta tatataatcc aactcttctt ctaaggtttc tggtggtaac aggagttctg
     1681 cttcatccaa aactaaaaat tctgtctcct tattaacttg tcccatcatc aaaagaatgt
     1741 cttttgcatc tcctggcact gcttcccgca agatgatccc aacttcttca ctcatgattt
     1801 tcagcccatt tttctagaat tcgctgtttg aaggtttcac tttgctcccc gtatgagatt
     1861 agtttcacat accgcttttc taaatctgta tcactttttt ctaaaatcag ttccaaataa
     1921 tcctccggca gtgcttcttc taatagattt ccccattcaa caacggaaag cccatcacct
     1981 tcaaaatatt catctaaccc taaatctgct ccgctagctg ctacccgata aatatccata
     2041 tgatacaacg gaatccgacc ttgactatat tctctaataa tcgtgtatgt cggacttttg
     2101 atcatttgcg ttatccctaa tccttgggca attcctttcg tcaacgttgt tttacccgca
     2161 cctagatcac cagtcaatat cagattgtca cctggcattg cggaatgacc aattgcctct
     2221 gcgaattgtt ctgtcattgt taatcctgat agttcgatca tttttcatct tctcccgctc
     2281 ttctttcttt ttgccagtat atcgcgtaat gaagtttttt taaagaaaaa atgaactagt
     2341 caagaacagc agctgctgtt tttggctagt tcgcgtgtat ttgttaatca atcaatgttt
     2401 gtgctgctgt gataatcgat aatttatata catcttcttc atttgctcca cgagacaaat
     2461 cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac
     2521 gctgagcaat tttatagccg atatttcctg actgcaattc tggaaataca aagactgttg
     2581 cttgaccagc tacttctgaa tctggcgctt tcagttgtgc aactgaagga acataagaag
     2641 catcaaattg caattcgccg tcgatcatgt attgtggcgc tagtttttta gcgattttag
     2701 ttgcttcaac tactttgtct acttctggcg atttcgctga gccttttgtc gagaagctca
     2761 tcattgctac ttttggttcg atatcgaaca attctgctgt ttttgcagaa tctacggcga
     2821 tttcagccaa ttcttgcgca gttggattca cgttgatcgc acaatccgaa aagacatatt
     2881 tttcttgatc tcttccacgt accatgatca tcgctccgct tgtccgactg atgccaggct
     2941 ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag
     3001 agaccattcc gtctgcaatc cccatatacg taagcatcgt accaaagtaa ttcacatctt
     3061 ttaaaatctt ttctgcttgt tctttggatg cctttccatt tctacgttca acaaaagtat
     3121 ccaccatttc atcccactta tcgtaattat cagggctgat gatcgtaaag ctagatgttt
     3181 tgattcctct ggcatgcgct gcatttctta tttcttcttc gttccctact aaaatgggtt
     3241 ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa aatacgagca tcagtagctt
     3301 ccgggaaaac gattttgatc ccacggcgaa caattttaaa tcttaaacta tcaaacaatt
     3361 ccacaagatg acctccagac gtttttatat gtattatcat acactattcc agaaaaaaag
     3421 aacagtttat tttatctgta tcagtcaaaa atgctggaaa aattaagttt ttcacataca
     3481 aagcgaactg cactataaca ggaaagcgct gttttattac actgtttcag gcagttgcca
     3541 atcaatcggt tcttctccta acgcaaggag cgcatcattc gttttcgaga agggtttgga
     3601 accaaaaaag ccacgacttg cagataacgg actgggatgt ggagatgtta tgatgatatg
     3661 gcgactctta tcgatcattt tgattttttc ttgtgccggt tttccccaca agataaacac
     3721 gattggtttt tcccgttcat ttaatttttc aatgatcgtg tctgttaatc tttcccaacc
     3781 tttcccgcga tgagaatacg cttgcccttc ccgaacggta agaacggtgt tgagtaaaag
     3841 gactccctgt ttagcccatg acactagatt tccatgttga acaggtgcga ttccaagatc
     3901 atcataaagc tctttataga tatttcttaa cgatggcggg atctttacgc caggttgcac
     3961 agaaaaagat aagccatgag cttgattttc tccatgataa ggatcttgtc ctaaaatcac
     4021 cactttaact tcttcataag gagtcaattc caatgcttca tagatatgat acatatctgg
     4081 atggatcttt tgggacgcat attctttttt taaaaattct cttagatgca gataataatc
     4141 ttttgaaaat tctgaggaca aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa
     4201 ctccacctcc taataaaaag aataactcac attttcttca atcacaaggg acaatcttca
     4261 ccctcagcac ttgattaata tggtaaacta ggaacaagaa gcagaaagga acgataatca
     4321 ttatgatcaa attaattgca tcagatatgg acggcacatt attagatgcc catatgagtg
     4381 tttctcctga aaatgccgag gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg
     4441 tcgctactgg tcgaaactat caagaagctc gcgcagcttt agatgaggta ggaatcgatt
     4501 gtgcgatgat cactttgaac ggggcacagg tatttgataa agaaggaaat tcattgttta
     4561 ccgtgtcgat tccaaataat caagcaatct ctgttttaga tattctagat gctaacggta
     4621 tttactatga agtagcaacc aacgatggtc tttattctga aaatcaagcg aaacgaattg
     4681 aaagttttgc ttccatggtg gctacccatt tacctcacct tacgtataaa atggcgattg
     4741 ccatggcctc agctaagctg gagttattgc acatcacata cgtggacagt attcgtgcta
     4801 ttttggaaga aaagaatctg gaagttttaa aaattatttg ttttcataca gaagggccag
     4861 ctgtattagg tccagttgga aagcagattt ctcttttagg cgatctagcc gttacttcct
     4921 ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca aaaaggaatt gctgttgccc
     4981 atgtcgctca cgagcgtggg atttctttag aagaagtcat gacgattggc gataacttca
     5041 atgatgtcag catgctgcaa gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg
     5101 aagtaaaaga ttatgccaaa tatacaacgg acacaaacct tgaatctggc gtaggaaaag
     5161 ccatattgcg cgcgattgca gaagacttgt agttttatct agaaaagagg gatatagatg
     5221 tcagaatttt tcattcagga aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac
     5281 gaaaaaggac attccctgtt tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg
     5341 tctctttatg ccatgaatgg cgatctcgtt gctcacatca agcagataag cctgacattc
     5401 ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg gaaccatgca gaaaatattc
     5461 aattggcccg gtgactttta ttatattcgt agacttcact ggacagtcca aggtgatatc
     5521 tataatcatc aatatcaaat ccatcatttc aatcagcaaa tcatgaaaat ggataaagca
     5581 acactttttt caggagatta ctatgtattg gatgtctcaa atgatgaaga tgctccagtt
     5641 tgtatttgta ttgctgcggt tttagattac tggctctacc gtaaagacaa aagtcgcgat
     5701 aagcctgttt cttaccgact taacttaagt taaaaagatg tacctgaaat gcggaaatac
     5761 gcatttcagg tacatctttt tagatattct tttgcatatg atcataaaga tgaccactga
     5821 tcatccgctg accgacaact ttgaagtcat ggcgttcgta taatttttta gcctttggat
     5881 ttttttcatc cacacttaga ccgatgacgg agcgattagc tttcttcgcc aatttaggta
     5941 atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa atcttctgat acactgatag
     6001 agtccaaata ccattcgttg ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat
     6061 ctagctgata tttctctaag ataggtacca gtggctgatc gatgattgat tcttcttcat
     6121 ctgtataacc gtaagcaact cctgcaactt taccgtctat ttcttctacg atacctcgtg
     6181 aataactgta acgataattt ggatcagtga ttgcttcttc taaaatctct aaagtctttt
     6241 gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt tagaatcact aagatcaatg
     6301 gggcgatttc tggagcatct tcttttctcg caaaacgaat catagtttcc tctccttttc
     6361 tccattctaa catgcagaaa aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct
     6421 tttcagtaat gaaataacca tgagtaacga ttcttgccgc ttcagttcgg caataagggc
     6481 agacgactgg catttcttta tcattatgtt cgactacatt attgacttct tcgtatgcaa
     6541 atttttttcc gcaattaggg catttcattt tttccatgtt atctcaactt cctttcatct
     6601 ttactctctt agcttactct tatattgtaa aaaagagaag caatatcctt cttctatgca
     6661 gatatccata cctgacggtc ctgttctcca tctattaatc ggactttata ataaggacgt
     6721 tcatctttga ctagaagtgt ccactctttc acttttgctt ttttcccgta gtagctgact
     6781 gcttccttca tcgctgcttg cggttgtttg aactcttccg ttttttctat atcaaaaaga
     6841 tgttctgcgg ccttttccgt gtcatttcgt acggttgttt tcccagtgga tggattgact
     6901 gctatgactt gtccttcaca aaaaaactgg tattcatatg ttgcattcgt aggattctcc
     6961 aaatcttttt caactgcaaa atgaatagca gatatgttat gttcttttgt ttcgtttttt
     7021 acgattgctg ctgctttttt caatgagatc cgatattcct ggtcactaat atcatccatg
     7081 tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc ctattacgat gagggcagca
     7141 acaccaaaca tcattttttt cattcttcat tctcctgttt ctttttttta aagaatatca
     7201 agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt
     7261 attcgtgcag ttctaatggc aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg
     7321 taaaggtatc ttctctaatc ggttctgatt cgatccctaa ggactgcaat tctgcaattg
     7381 tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa gccacaagct tctggatagt
     7441 ttggtcgttg tggcgcatta gcaattccaa aaatctccaa ttcagcactt cctagtttca
     7501 gatctaattt atagtcatct cgttctttgc ggtaattttc tctgattact tcaaatccta
     7561 atttattaac ataaaaatca cgtgatttct gataatctga aacaatgatg gctatatgat
     7621 ggatcgttgt aaggttcata tgagcactcc tattcttcgt ttaagctcgg atctatatta
     7681 atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat agctgttttt cctttttata
     7741 agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga cactcttcct gttcccaata
     7801 taataaatga aagtaagaaa ttatattttc aagaaaacaa acagaaaatt atcataacac
     7861 aaattattta taattgaaag actagttttt tttcaagaaa agcatacaat agattgtgtt
     7921 agtaaataca gaacacacta catttagtgt cttaaggagt cctcatacat gaagtcatat
     7981 tttcagtttt tagctcatca gctaaaaggc tggccgcaac aaaattatta tcttttcttt
     8041 ttcagtcttg gctgccaggt catgactctt gtcaatagtc ccatcacttg gctgtccgtt
     8101 attacattta tcggtaccac gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa
     8161 tcagtcaacg gtattttagg tattttgtct gcgatttgtt ttattatcgt tgggttttca
     8221 gcaaagaact atttaagtat cggtgaacag attgcttata tgatcacatt ggatatcccc
     8281 gtattgttaa gtaaagaatg gaatcataat atggcttcaa aaatccgaaa attcactgga
     8341 aaaagctggt ttatcgcaat catcagtact ttccttgtct atcttgtttc aggattcctg
     8401 attggtcgtt atacagatga tcctcggccg tgggtagatg ccattgcttt ttctattagc
     8461 ttgactgctg gtatcatcag tttcttgcgt tacaacaacc aatattactg gtggttagct
     8521 tcagggcttg ctcaaatggt cctttggtat atttcattta cgcatggatc tgcaagtttg
     8581 gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg tccttgcatt taccgtttca
     8641 ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa tggaagaaga atactacaac
     8701 gcacaacaac acgcataaaa aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc
     8761 acgctctaaa cacgtataaa cggcggaatc agaagcaact ccttcggaaa taagctgaaa
     8821 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
     8881 ttctgtccca gcctctattt cctacgatta gtcaagtaaa aggtgcaaga aaaatagctc
     8941 gtaaacgaaa gcgacaaaat caaagcacac caaataaagc cccgttcgta aaaaattttc
     9001 gatctgggca aagaagtggt atactgtaag tggtttagtt aatggtccac tgagaccgcg
     9061 tacgcatagt cgtactctgt atgtgcccta accatagcgt gcatacattt cagaagcttg
     9121 ttcatacaag caactgtggc aaccttatct ttcttaggga taggttgctt ttttaatttg
     9181 tagtaataat cgacaatatg attaggagct gcgcgttgct ggcgaatcat atttcgaatg
     9241 gtgaagaaaa taattttccg tgctttggga ttaccacgct tattgatgcg gtcttggcca
     9301 gtatacttac ctgaatgata ccgtcggatg tcgattccga caaaggcatt caattgttta
     9361 tgggttttaa atcgtgtgat atctcctaat tcaccgagca ataaagcggc tgtgagtgcg
     9421 ccaatcccag ggaacgtttg gtacaacaaa aagcaaggag agccttcggc ttttttgatc
     9481 atttgtgtcg ccagtgcttt tttttgttcg agtaaatctt ggagaagttc ggcgtaatag
     9541 atcgtttttt gacagtggat actgtctttt tcaacagctg gataggcaca ctgtgcatag
     9601 tggatgattt gatctgcctt ttgctcggca cgattcgcgg agattttttt ccgtgtttcg
     9661 ttgatcagtt tattttttat ttttgtccga gtagatgcca atacaaaatc tggatgggga
     9721 aaaagacgga tcaatgtcaa tgcgtaaggg gtcagcctgt ttgaaaagaa ctgttcaagc
     9781 tctggaaaag tcaattgaag gcagttgtgt aaatccatgc gcagacgagt gattttcttc
     9841 tcaatttctt gataaaaacg ggctaaatcg cgcatctcta agtaaaaatt gctttgtact
     9901 tcttttggct gacgttgatt tttaagatga gtctgtgcga gccggtgggc atccgattta
     9961 tctgttttcc aactgcgtaa ggtgctttct tccatctgtt ttttcgcctc taacgggttt
    10021 aacaaacagt aagaaaagtg atgatcttga aagaactttt cgactggtct ggaataaatc
    10081 cccgttgctt caaaaacaat caatggacgc tcaggcaagt gctggatctc ttcgagtaat
    10141 gcatgaaatc ctctttgagt gtgttcaatc tcgtcttcaa ataagcaggt gtcgtctttg
    10201 tataggacac tgtagctgtg tcccattgaa acatctaatg caagaatgta aggcatttgt
    10261 tttcctcctt tttaagtttt gaaagaactt tactgcttca tttcgattcc aatttcctat
    10321 acacgggctc atggccccaa catacgtaaa cctgattcta aaatgacagt aaagaagttc
    10381 ggtttttgat agggactcgt agtcctcaag gctttgttcg aactttcttt cactcttcac
    10441 tataatctaa aaatagaaaa tagaggagaa catcattcgt caatgatgtt cccctctaat
    10501 cttagtatgt tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca
    10561 gtgattcttc taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat
    10621 aatgttttcc tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca
    10681 ctactagttg ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag
    10741 cttctcgttc tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt
    10801 cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt
    10861 ttggttggat gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg
    10921 ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat
    10981 gtactggcac ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt
    11041 tgatcaagaa ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt
    11101 tttttatctg tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat
    11161 tttccaatgc ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga
    11221 gccgctgagc taattttata tccggtaaat atggctgttt catccgacga ttttggtaca
    11281 agacaaaaca gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc
    11341 ataataattc gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca
    11401 ctgtcggaga gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc
    11461 cggtttttgg cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca
    11521 atttttgctg gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc
    11581 aatctgttgc agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt
    11641 gcttatcgac tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac
    11701 tgcttgtgta ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc
    11761 attcttcaaa gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa
    11821 agctggctac taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg
    11881 ttccggcatt tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca
    11941 aagaagaagc aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt
    12001 gatcgggtga gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga
    12061 tcaaatgaag caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga
    12121 tgtacgctgg tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg
    12181 taaagtaagc ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat
    12241 tatcagggat tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc
    12301 gcccacctgc ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt
    12361 tgactacttg actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac
    12421 cgctgatttc atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac
    12481 caaatggatc aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt
    12541 aacatatggt tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat
    12601 agtttaaata acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact
    12661 acaatgttag aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa
    12721 atagaatcat ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt
    12781 tgatcactcc gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag
    12841 cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta
    12901 acgctggatt cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg
    12961 tgatggccgc aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa
    13021 tgatgatgcc gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca
    13081 ctgtcccaac tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca
    13141 tgcttaacgg ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc
    13201 gattatctcg agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc
    13261 cgactaaagc acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca
    13321 aagctggaat aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg
    13381 atggaagccg gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa
    13441 agatcaagcc accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta
    13501 agaccgtcca gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc
    13561 ttactccagt gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat
    13621 cgcctaataa aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa
    13681 tatttcttcg ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc
    13741 gtgatagcca ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga
    13801 tctttcagtg ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca
    13861 aagaaccagc agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta
    13921 ggatggactt ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga
    13981 cgaacaaatc cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag
    14041 atcccataaa tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa
    14101 gccgctgatt ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata
    14161 aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga
    14221 atgccaagac cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag
    14281 tctaatttaa atgcaaaccc gagaataaca atcaagacgc ctaaaaggct aagataattt
    14341 tccatatatt ttcctccata aaatgacaat atttttttaa ttataaagca aaacacataa
    14401 aaaatatagt tgttttctca cgaaatagac actattttta aatatttttc gttatttttt
    14461 tatcacaaaa attatcttac ttat
//
LOCUS       DANKAY010000064        14345 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000064 DANKAY010000000
VERSION     DANKAY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..317)
                     /locus_tag="PWY98_001921"
     CDS             complement(<1..317)
                     /locus_tag="PWY98_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2037665.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKI"
     gene            complement(317..547)
                     /locus_tag="PWY98_001922"
     CDS             complement(317..547)
                     /locus_tag="PWY98_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2037666.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            834..1172
                     /locus_tag="PWY98_001923"
     CDS             834..1172
                     /locus_tag="PWY98_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037667.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1377..1616)
                     /locus_tag="PWY98_001924"
     CDS             complement(1377..1616)
                     /locus_tag="PWY98_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2037668.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1592..1939)
                     /locus_tag="PWY98_001925"
     CDS             complement(1592..1939)
                     /locus_tag="PWY98_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2037669.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2671..3252)
                     /locus_tag="PWY98_001926"
     CDS             complement(2671..3252)
                     /locus_tag="PWY98_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2037670.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3435..4061)
                     /locus_tag="PWY98_001927"
     CDS             complement(3435..4061)
                     /locus_tag="PWY98_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2037671.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4083..5414)
                     /locus_tag="PWY98_001928"
     CDS             complement(4083..5414)
                     /locus_tag="PWY98_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2037672.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5569..5970)
                     /locus_tag="PWY98_001929"
     CDS             complement(5569..5970)
                     /locus_tag="PWY98_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2037673.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6056..6262)
                     /locus_tag="PWY98_001930"
     CDS             complement(6056..6262)
                     /locus_tag="PWY98_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037674.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6357..7859)
                     /locus_tag="PWY98_001931"
     CDS             complement(6357..7859)
                     /locus_tag="PWY98_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2037675.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7877..8332)
                     /gene="argR"
                     /locus_tag="PWY98_001932"
     CDS             complement(7877..8332)
                     /gene="argR"
                     /locus_tag="PWY98_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2037676.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8430..9047)
                     /locus_tag="PWY98_001933"
     CDS             complement(8430..9047)
                     /locus_tag="PWY98_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2037677.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9268..10641
                     /locus_tag="PWY98_001934"
     CDS             9268..10641
                     /locus_tag="PWY98_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2037678.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10716..11828
                     /locus_tag="PWY98_001935"
     CDS             10716..11828
                     /locus_tag="PWY98_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2037679.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11900..13537)
                     /locus_tag="PWY98_001936"
     CDS             complement(11900..13537)
                     /locus_tag="PWY98_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2037680.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13705..14313)
                     /locus_tag="PWY98_001937"
     CDS             complement(13705..14313)
                     /locus_tag="PWY98_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2037681.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3888 a         3003 c         2540 g         4914 t
ORIGIN      
        1 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta
       61 aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt
      121 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac
      181 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
      241 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
      301 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
      361 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
      421 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
      481 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
      541 caagcattat catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat
      601 tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa
      661 tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat
      721 tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt
      781 ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg
      841 attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca
      901 cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag
      961 aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg
     1021 tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat
     1081 ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga
     1141 attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa
     1201 aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga
     1261 ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga
     1321 tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat
     1381 accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata
     1441 tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt
     1501 ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc
     1561 tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat
     1621 ttgtcttttt cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat
     1681 gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt
     1741 taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc
     1801 ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct
     1861 tcggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa
     1921 aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac
     1981 aagaagttcg caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag
     2041 tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat
     2101 tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca
     2161 ggcttacgac ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt
     2221 tatgcaaaaa tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga
     2281 ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
     2341 aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg
     2401 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctaacctt cggtattagc
     2461 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
     2521 aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata
     2581 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     2641 gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct
     2701 ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa
     2761 taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc
     2821 ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt
     2881 gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa
     2941 attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc
     3001 caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa
     3061 ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga
     3121 agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat
     3181 tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc
     3241 ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat
     3301 tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc
     3361 tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa
     3421 aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct
     3481 gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt
     3541 gcatctgtcg ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg
     3601 ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat
     3661 tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta
     3721 ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact
     3781 gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca
     3841 ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg
     3901 tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt
     3961 tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc
     4021 gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt
     4081 atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct
     4141 ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca
     4201 tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa
     4261 actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata
     4321 tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca
     4381 ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt
     4441 cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa
     4501 gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga
     4561 aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat
     4621 ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt
     4681 gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact
     4741 tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc
     4801 atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca
     4861 actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg
     4921 atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag
     4981 tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg
     5041 aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg
     5101 ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata
     5161 tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta
     5221 aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg
     5281 atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt
     5341 gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga
     5401 tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga
     5461 taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa
     5521 aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt
     5581 tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc
     5641 ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac
     5701 accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga
     5761 ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc
     5821 ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt
     5881 gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt
     5941 gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct
     6001 acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt
     6061 cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga
     6121 acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat
     6181 gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa
     6241 aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga
     6301 tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat
     6361 tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac
     6421 gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt
     6481 aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata
     6541 ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt
     6601 tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt
     6661 tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt
     6721 ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga
     6781 aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc
     6841 ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga aatataacga
     6901 acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc
     6961 gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa
     7021 ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta
     7081 tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc
     7141 gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat
     7201 tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca
     7261 gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt
     7321 ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct
     7381 ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca
     7441 atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag
     7501 ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg
     7561 gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct
     7621 ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca
     7681 atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt
     7741 gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca
     7801 actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata
     7861 taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt
     7921 cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt
     7981 cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca
     8041 atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac
     8101 gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt
     8161 tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt
     8221 gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag
     8281 gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca
     8341 cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt
     8401 acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc
     8461 gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct
     8521 ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct
     8581 ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt
     8641 ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct
     8701 tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt
     8761 tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt
     8821 ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt
     8881 attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg
     8941 tcgccgatcc ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt
     9001 tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg
     9061 cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt
     9121 tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac
     9181 gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt
     9241 ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct
     9301 gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat
     9361 gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta
     9421 acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc
     9481 aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg
     9541 atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca
     9601 gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat
     9661 cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc
     9721 cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt
     9781 gtgcccgttt tgtttctttc tttgattatc ggctggtttg ctattcccga gatggcagta
     9841 caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga
     9901 agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc
     9961 ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga
    10021 ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag
    10081 tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt
    10141 agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct
    10201 cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg
    10261 attctttctt tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt
    10321 gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg
    10381 atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc
    10441 aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat
    10501 tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca
    10561 gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg
    10621 ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg
    10681 tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg
    10741 gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt
    10801 tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt
    10861 tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc
    10921 ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt
    10981 tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag
    11041 aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc
    11101 cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag
    11161 catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa
    11221 aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca
    11281 cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag
    11341 gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata
    11401 agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca
    11461 tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc
    11521 ctactagcga agcttataag ggattgacta agagtacaga acagttcttg actgatccag
    11581 atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt
    11641 tttccccatt tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag
    11701 gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata
    11761 ctggtcattg gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc
    11821 catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact
    11881 tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg
    11941 cttcatctgg caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa
    12001 gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta
    12061 taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca
    12121 cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc
    12181 cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat
    12241 agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat
    12301 ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac
    12361 caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt
    12421 tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg
    12481 cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt
    12541 tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg
    12601 ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag
    12661 caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac
    12721 cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag
    12781 ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa
    12841 agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa
    12901 agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat
    12961 ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa
    13021 aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca
    13081 aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa
    13141 attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat
    13201 acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga
    13261 tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa
    13321 catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc
    13381 ctggactccc ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat
    13441 taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg
    13501 tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca
    13561 aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg
    13621 aagcaaacga gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg
    13681 tttcatcatt ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc
    13741 agcatcccat gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc
    13801 agctgaatag tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc
    13861 gttagtagct gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct
    13921 tgaagttggc gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg
    13981 tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt
    14041 tttgttttct gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac
    14101 ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt
    14161 gtctcctgca aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac
    14221 ttcatcagcg tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt
    14281 tgtacctaaa acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt
    14341 tattc
//
LOCUS       DANKAY010000065        14128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000065 DANKAY010000000
VERSION     DANKAY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            237..1877
                     /locus_tag="PWY98_001938"
     CDS             237..1877
                     /locus_tag="PWY98_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2037682.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="PWY98_001939"
     CDS             2031..3353
                     /locus_tag="PWY98_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2037683.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="PWY98_001940"
     CDS             complement(3415..3846)
                     /locus_tag="PWY98_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037684.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="PWY98_001941"
     CDS             complement(3910..4746)
                     /locus_tag="PWY98_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2037685.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="PWY98_001942"
     CDS             complement(4936..5238)
                     /locus_tag="PWY98_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2037686.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="PWY98_001943"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="PWY98_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2037687.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="PWY98_001944"
     CDS             complement(5692..5958)
                     /locus_tag="PWY98_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2037688.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="PWY98_001945"
     CDS             complement(6212..6937)
                     /locus_tag="PWY98_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2037689.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="PWY98_001946"
     tRNA            complement(7303..7373)
                     /locus_tag="PWY98_001946"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="PWY98_001947"
     CDS             7490..8242
                     /locus_tag="PWY98_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2037690.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="PWY98_001948"
     CDS             8258..8737
                     /locus_tag="PWY98_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2037691.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="PWY98_001949"
     CDS             complement(8988..10568)
                     /locus_tag="PWY98_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2037692.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="PWY98_001950"
     CDS             complement(10561..11304)
                     /locus_tag="PWY98_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037693.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="PWY98_001951"
     CDS             complement(11480..12256)
                     /locus_tag="PWY98_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037694.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="PWY98_001952"
     CDS             complement(12504..13340)
                     /locus_tag="PWY98_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2037695.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..>14128)
                     /locus_tag="PWY98_001953"
     CDS             complement(13351..>14128)
                     /locus_tag="PWY98_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2037696.1"
                     /translation="ENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAF
                     MLTAPIINPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKIT
                     SKVAENHHHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAM
                     QTYLPTRIMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLV
                     FGPMVDIKNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
BASE COUNT         4327 a         2806 c         2472 g         4523 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttca
//
LOCUS       DANKAY010000066        13807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000066 DANKAY010000000
VERSION     DANKAY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWY98_001954"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWY98_001954"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2037697.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="PWY98_001955"
     CDS             complement(2260..2511)
                     /locus_tag="PWY98_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2037698.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="PWY98_001956"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="PWY98_001956"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2037699.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="PWY98_001957"
     CDS             3461..3679
                     /locus_tag="PWY98_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037700.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="PWY98_001958"
     CDS             complement(3968..4480)
                     /locus_tag="PWY98_001958"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2037701.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="PWY98_001959"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="PWY98_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2037702.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="PWY98_001960"
     CDS             complement(6933..7376)
                     /locus_tag="PWY98_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2037703.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="PWY98_001961"
     CDS             complement(7377..8165)
                     /locus_tag="PWY98_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2037704.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="PWY98_001962"
     CDS             complement(8162..9103)
                     /locus_tag="PWY98_001962"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2037705.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="PWY98_001963"
     CDS             complement(9280..9867)
                     /locus_tag="PWY98_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2037706.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="PWY98_001964"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="PWY98_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2037707.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="PWY98_001965"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="PWY98_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2037708.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="PWY98_001966"
     CDS             complement(12784..13719)
                     /locus_tag="PWY98_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2037709.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DANKAY010000067        13537 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000067 DANKAY010000000
VERSION     DANKAY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(127..1680)
                     /locus_tag="PWY98_001967"
     CDS             complement(127..1680)
                     /locus_tag="PWY98_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2037710.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1828..3117
                     /locus_tag="PWY98_001968"
     CDS             1828..3117
                     /locus_tag="PWY98_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2037711.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3243..4322)
                     /locus_tag="PWY98_001969"
     CDS             complement(3243..4322)
                     /locus_tag="PWY98_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2037712.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4431..4871)
                     /locus_tag="PWY98_001970"
     CDS             complement(4431..4871)
                     /locus_tag="PWY98_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2037713.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4928..5338)
                     /locus_tag="PWY98_001971"
     CDS             complement(4928..5338)
                     /locus_tag="PWY98_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2037714.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5519..5953)
                     /locus_tag="PWY98_001972"
     CDS             complement(5519..5953)
                     /locus_tag="PWY98_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2037715.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(6053..6901)
                     /locus_tag="PWY98_001973"
     CDS             complement(6053..6901)
                     /locus_tag="PWY98_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2037716.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            7290..9056
                     /locus_tag="PWY98_001974"
     CDS             7290..9056
                     /locus_tag="PWY98_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2037717.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            9158..10417
                     /locus_tag="PWY98_001975"
     CDS             9158..10417
                     /locus_tag="PWY98_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2037718.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            10564..11865
                     /locus_tag="PWY98_001976"
     CDS             10564..11865
                     /locus_tag="PWY98_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2037719.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            11868..12719
                     /locus_tag="PWY98_001977"
     CDS             11868..12719
                     /locus_tag="PWY98_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2037720.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            12732..>13537
                     /locus_tag="PWY98_001978"
     CDS             12732..>13537
                     /locus_tag="PWY98_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2037721.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKR"
BASE COUNT         4592 a         2416 c         2483 g         4046 t
ORIGIN      
        1 caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata agaaggatgt
       61 tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt atgtatctta
      121 tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg cttcttcttt
      181 taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt ctggtgtgat
      241 gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca aagggcagtc
      301 gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg attcatttga
      361 aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac gcgtatagtt
      421 cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta ctaggtcttc
      481 aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt gattttgttc
      541 tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca cgctatgttc
      601 ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa gaaatccatt
      661 ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt caggtataaa
      721 caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa catgaggaac
      781 gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt cgattttcgt
      841 gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa ttggcagatt
      901 aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat actctgtgaa
      961 ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata tttttaatag
     1021 aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt ttttaaaaaa
     1081 agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg tagcagtact
     1141 tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct cttttccaga
     1201 aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg ctaaaaatgc
     1261 attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt gctgacttaa
     1321 actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat aactgactga
     1381 ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa ttccttcaaa
     1441 attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat tgagacgctc
     1501 aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca tatggaaatg
     1561 atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa gaaaaatatc
     1621 taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt gagtttgcat
     1681 gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt ttttttcata
     1741 aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta tttcttgcat
     1801 acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat atcaacattt
     1861 gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga gatagaaaca
     1921 gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca ggatgaattt
     1981 tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac catcaaagca
     2041 aaaattgact attcaggaga cttagcacct atactacaat cagctgttac atttgagaat
     2101 gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca acacaagcaa
     2161 gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc gctttttact
     2221 gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta tagtcagcgg
     2281 cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga agaaccaaca
     2341 gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa
     2401 cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac
     2461 aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc agaagaaaaa
     2521 ccggatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat
     2581 ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggcagcccc agaagaaaaa
     2641 ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat
     2701 ccagacacag ataatgaaac tgaaaatcca gaaaaactag aggtagatcc agaagaaaaa
     2761 ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata
     2821 aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt
     2881 ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca
     2941 gctgaaacct taaaaagtag caaggataca acctttgcta gtcctgaaac gaaaaataaa
     3001 cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg aatcattcta
     3061 ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc tctctaacat
     3121 ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa aacgacccca
     3181 aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc ttcatattta
     3241 ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac tttcaataca
     3301 tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc aaagaaccgt
     3361 tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt gctggacatc
     3421 aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt aatcaggata
     3481 taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg aatacaagaa
     3541 atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc taaactcagc
     3601 agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtgtt taaaagagcg
     3661 ataacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc ggcaaaatag
     3721 taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga gaatacaatt
     3781 gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc aaacgccact
     3841 gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt ttgaagctgt
     3901 tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg atctgcatct
     3961 tttggctgat gctcgtaaaa atcaataacg gactggatca tatcaaatgt ttgatgaata
     4021 agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag tgtataaatc
     4081 atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg aacaagatga
     4141 atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact tctaactaag
     4201 aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc attcgccaaa
     4261 aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa cacgttattc
     4321 attatatgat ggaatatgaa aataagaaag agaaagagca aaaagaaaaa ccgctaaatg
     4381 acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt
     4441 ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat
     4501 tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt
     4561 tgcaacgcag caccttgagg agtcagcgta acatttacga ttctttcatc acccgtttca
     4621 cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca
     4681 gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa
     4741 actaacatca caagatactg ggtataggta agattcagcg gcttgaggag ggaagtgtag
     4801 tgtctaatga tttccttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat
     4861 agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca
     4921 aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct gtagcttttg
     4981 aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac
     5041 cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa
     5101 gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt
     5161 ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg
     5221 cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat
     5281 gaacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa
     5341 aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag
     5401 caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct
     5461 cgaatagcaa acatatacaa aaaaatatca gagacaaaaa ctaatctcat cataaacatt
     5521 acatatattt ctcaaaaagt tgttttcttt cttcaaaact tacttcacga tggacctgat
     5581 gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca
     5641 tcatttctgt catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt
     5701 cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag
     5761 cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtgcca tacaaggtaa
     5821 aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa
     5881 agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa tctatttccg
     5941 ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat
     6001 tataccagtt aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga
     6061 ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg
     6121 cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa
     6181 cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa
     6241 ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca
     6301 tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag
     6361 tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaaca aacagattaa
     6421 gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt
     6481 ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa
     6541 tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag
     6601 taatcattgt ttctggaact attgcaatga ttaaaggaaa aaacaagctt ccgaaaagta
     6661 ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata acgatattca
     6721 acactaaaat aactatagct cggtcaaatc ctaaaacagc ttccagtaat attcctagac
     6781 cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta
     6841 atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca
     6901 tttatccact tccttaattc attaataata ctcttatctt attataactt acttttcacc
     6961 ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt
     7021 ctagaatata cttctacgtt aaaaactgtt aaaaaacttt tactttagaa aattggagaa
     7081 attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagat taaaactttt
     7141 aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac
     7201 tgttaccgat aacatttttg aaagcgttgt cttttgtttc tctatcactt atagtgatga
     7261 cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg
     7321 aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga
     7381 aaaatgatat caaaaaagta tatgtgcttt gtggagatcc atataccata acaactgaaa
     7441 aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt
     7501 gggaaattga agtgacgagc gaaacaaggc gcttacagta tgcgttccat gttgttgggg
     7561 aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga
     7621 ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta
     7681 ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg
     7741 gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag
     7801 aagacttctt cgggggagat ttgcaaggtg tgagggatca tttggattac ttgacggacc
     7861 ttggcgtcaa tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg
     7921 atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact
     7981 tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata
     8041 tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata
     8101 aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag
     8161 aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa
     8221 atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag
     8281 aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga
     8341 aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt
     8401 ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat
     8461 tcacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca
     8521 gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact
     8581 tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga
     8641 tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga
     8701 cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga
     8761 aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac
     8821 agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtatat gatcgtgaag
     8881 ataagctcta tttcacacgg gaactagagg gacagatcat ctatgtttac tttaatcagt
     8941 caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt
     9001 tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac
     9061 tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg
     9121 gtgtaacagc acgttgaata cacaggagga aatgaagatg aaaaaaagaa gtgtgaaaca
     9181 gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg
     9241 taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc
     9301 tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa
     9361 aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc
     9421 tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca
     9481 gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa
     9541 agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt
     9601 tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa
     9661 tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt
     9721 agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt
     9781 atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga
     9841 aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat
     9901 aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat
     9961 tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa
    10021 aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt
    10081 agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa
    10141 tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc
    10201 tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga
    10261 aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa
    10321 cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt
    10381 aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt
    10441 tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct
    10501 tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt
    10561 cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt
    10621 attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc
    10681 atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg
    10741 gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact
    10801 ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct
    10861 tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc
    10921 atacataata taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc
    10981 atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat
    11041 gacttcaatc atattccacc agccagttta attgattggg taggggctaa aaactttttt
    11101 agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg
    11161 atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt
    11221 gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg
    11281 gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt
    11341 gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca
    11401 atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg
    11461 ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac
    11521 ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg
    11581 cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc
    11641 aataatttct ccatgatcta tctgtttaac aatggtggtc ccggaagcgt aggcggagga
    11701 gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg
    11761 cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca
    11821 ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc
    11881 attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa
    11941 tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag
    12001 gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga aactttactc
    12061 ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc
    12121 taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc
    12181 gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta
    12241 tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact
    12301 ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag
    12361 gatactttga cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc
    12421 atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag
    12481 cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa
    12541 cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc
    12601 aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat
    12661 tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg
    12721 agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc
    12781 aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt
    12841 tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc
    12901 cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt
    12961 ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc
    13021 ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta
    13081 ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct
    13141 atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc
    13201 aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata
    13261 gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct
    13321 ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg
    13381 caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta
    13441 tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt
    13501 tatgggtttt tgtcaaaaca agattcaaag ataagcg
//
LOCUS       DANKAY010000068        13236 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000068 DANKAY010000000
VERSION     DANKAY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(434..1048)
                     /locus_tag="PWY98_001979"
     CDS             complement(434..1048)
                     /locus_tag="PWY98_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2037722.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1654..2031)
                     /locus_tag="PWY98_001980"
     CDS             complement(1654..2031)
                     /locus_tag="PWY98_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037723.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2331..3212)
                     /gene="galU"
                     /locus_tag="PWY98_001981"
     CDS             complement(2331..3212)
                     /gene="galU"
                     /locus_tag="PWY98_001981"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2037724.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3255..4274)
                     /locus_tag="PWY98_001982"
     CDS             complement(3255..4274)
                     /locus_tag="PWY98_001982"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2037725.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4907..5911)
                     /locus_tag="PWY98_001983"
     CDS             complement(4907..5911)
                     /locus_tag="PWY98_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2037726.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5924..6973)
                     /locus_tag="PWY98_001984"
     CDS             complement(5924..6973)
                     /locus_tag="PWY98_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2037727.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6990..7811)
                     /locus_tag="PWY98_001985"
     CDS             complement(6990..7811)
                     /locus_tag="PWY98_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2037728.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7798..8577)
                     /locus_tag="PWY98_001986"
     CDS             complement(7798..8577)
                     /locus_tag="PWY98_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2037729.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8594..9064)
                     /locus_tag="PWY98_001987"
     CDS             complement(8594..9064)
                     /locus_tag="PWY98_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037730.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9076..9495)
                     /locus_tag="PWY98_001988"
     CDS             complement(9076..9495)
                     /locus_tag="PWY98_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2037731.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9576..12353)
                     /locus_tag="PWY98_001989"
     CDS             complement(9576..12353)
                     /locus_tag="PWY98_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2037732.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12544..12768
                     /locus_tag="PWY98_001990"
     CDS             12544..12768
                     /locus_tag="PWY98_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037733.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3887 a         2757 c         2149 g         4443 t
ORIGIN      
        1 gtattttatt tctattagaa aaaggtatcc tgttggacaa ttttgttata tcaggtaaac
       61 ttcattgcaa taatgataac aaatgtacta taattacttt gtaaagagtt ttggggaaga
      121 ctctttcatc aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc
      181 tttttccact actttttatt tttctgacac tacatattct tataactcat ttctcataac
      241 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
      301 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
      361 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
      421 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
      481 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
      541 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
      601 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
      661 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
      721 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
      781 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
      841 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
      901 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
      961 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
     1021 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
     1081 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
     1141 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
     1201 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
     1261 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
     1321 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
     1381 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
     1441 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
     1501 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
     1561 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
     1621 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
     1681 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
     1741 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
     1801 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
     1861 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
     1921 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
     1981 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
     2041 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
     2101 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
     2161 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
     2221 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
     2281 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
     2341 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
     2401 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
     2461 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
     2521 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
     2581 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
     2641 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
     2701 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
     2761 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
     2821 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
     2881 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
     2941 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
     3001 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
     3061 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
     3121 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
     3181 gcggcaggaa tgactgcttt tttgaccttc acttgctgtt cctcctaaac gacgattaac
     3241 tattgatcat ctatttaacg aagagaaaat tcattctcca tttttccgtc acgtaacatg
     3301 atttcttttg ctgctttttt gatatcttct ccattataca aaacattata gattgtttga
     3361 gtaattggca tctcaacacc tagctgttga gctagctcta ctgctgcttt tgtcgttgcc
     3421 acaccctcaa cgaccattcc catgttttct agaacttctt ccagcttatg tccctgtcct
     3481 aacaagttac cggcacgcca attgcgagaa tgaacacttg tacaagtaac gatcaagtcc
     3541 cccacaccac tcaaaccgat aaatgtcaga ggattggcac ccatcgcgac accaagacgg
     3601 ctgatttctg ccaatcctcg agtcatgatt gctgcttttg catcatctcc gaatcctaat
     3661 ccatggatag cgccggcacc aattgcaatg atatttttca gtgctgcacc agtttctacg
     3721 ccaatcacat cattatttgt ataaatcctg aaataatcat tcatgaacag tttttgtaca
     3781 taggctgctg ctttttgatc aatacttgca gctgtaattg ttgtgatatc atgtactgcc
     3841 acttcctcag catggctagg accagataat acaacaattg cctgtctttt ttcttctgga
     3901 atttcttcag ccaaaacttc tgagattcgt ttatgcgtat cttgttctag tcctttactt
     3961 gcatgtataa tgattggctt agtattcatt ttaccaataa gttcctgtgc gactgaacga
     4021 atcgcttttg ttggaacaac aaataaaata gcatctgcgt tatccactgc ttctgccaat
     4081 tctttatatc cgataatcga ttctgggatc gtcaaatcag gtaaaaaatg ctgatttgta
     4141 tgatacttat tgatttcatc aatctgttca ggtacatttc cccaaatacg gacatcatgc
     4201 ccgttttctg ccaatgtttg tgctaaagca gtgccccaag atccaggacc aagaactgcg
     4261 attttttgtt tcatggatat tcctccaaat aaataattga taatttccat tatagtatac
     4321 atttcttaaa gttgggacta atttccattg tttttaagtg tttgtttact tcttttatcg
     4381 tttttacttg aaataatcaa tgatatgtta caatagtcta cattttattt ttacactcgt
     4441 tatgctttgc ttgttcctaa gaggaagcag ttttgacaat gttactacca gcattactat
     4501 taggaggtta ggaaacttct cctagtgagt attgatttaa aatacataag agacagattg
     4561 aacatttcta aatctgtcag gtcctgcaac ggacagattg tctctgttga cggttgacat
     4621 aggaactttt gttttagtca acattctgga tacaaaacgc tatgtaaact ggcgcaactc
     4681 gttgtacttg gacttttact acggatccat gcaacaacta aatagtacta ttgcataaca
     4741 aaaaatggtt ggcacccaca tgtcccatta aacagattat aagttgaact gtagataatg
     4801 caaaaaatgt gttttataac gtaattgatg ccaatatttt aaaaaaataa agggagcagt
     4861 tatcgctgct ctctttattt tttatattct ttttatccag tctatcctaa taatcaaact
     4921 gacgatagta gcggcgtatc tctaatggat ggcagttcaa ctgctccaca tgagcatcga
     4981 tgcgttgtgt cactacatgc atcaataaga aagacaaaac acgtccgcga tatttttcac
     5041 tgataccttc tactggatag tcttttgtgt ctatcaatgt ataattgctg caaatttttg
     5101 gcaaaagttt agccacacgt tcagataaca ctctctgttc atcttcgcct aaaaataatg
     5161 tgactggggt tgtttcttct acgatctcta acgtcccgtg aagaaactcg gcagcatgga
     5221 tagatttcga tggaagccag ctttgttctt cccagtaaca catggcatat gaataaacag
     5281 ctccccattg attccctgct ccaataaaat aatgcatact atcacgtcga tgtttttctg
     5341 caaatgataa gccaaatgca tctgcttttt tctctgcttc cactaaccct gttggtaaat
     5401 aacgttcgag ctgttcatag tattctgaat aagcttcgaa ttctccattc aaatacatca
     5461 atcgatctgc tgccataaaa aatttgattt gttctgttcc aggagctgga taagtgacca
     5521 caaaatcgca tagctgagac aacttactgt tcgctttatc gataaatcca atcagtgtcg
     5581 ctcctacttt cttgatttgt tctactgctt tcactacttc ttgagttgta ccggtaacag
     5641 aggacaaaat aacgatagaa tcttttgtta atcttttgtt tccagtagtg taatattctg
     5701 cagcatgctg tacaaagaca ggcaagttgc ttttcccatt catataagtg acagcttgca
     5761 tcgaggaagc atacgttcca ccgattccta gataatagac agcgtcaaat ttttccgcat
     5821 aaagtttatc gatgatttct tctacttgcg gacgtaaagc tagtgcgcct tttattgctt
     5881 tgatttgttc ttcttcattg aatttcagca tgatttccac ctcttataat ttacttttca
     5941 agactgtatc aaagtcttca aaaatctttt tgttcaagtc aataccttta gctactgcgg
     6001 ttttataagc gaatagttga aacggaacga caagcgctaa tacagacaga tattcatcac
     6061 attctattgc taatccaagt gttttctcgt ttcttgcttt ttttgtggta atggtgagag
     6121 tctctccaac gtaccgactc atatatcttt gcaacttttg cgaacgttcc ttatttacac
     6181 tttccccttc aataaagaat agcaaatgcg aagcatctgc ctccaggtat gggccgtgca
     6241 tataagcttc taactcaaat ccttgtgacg gaacgcggat tgtttcagtc aatttcgtct
     6301 ctgcttcttt agctgttccc cagtttggac catatccaat ggtgacgaaa cgctgtgcta
     6361 aacgaaataa actttgatat ccatcaaaaa attcttccgt tttttgaata atcgctggta
     6421 aatggttgat tattttgcgc aactgttgca tgtagtcttg ttctattttt ttgctgattt
     6481 tatttttact tattccaatt cctatcccta agagtagtaa ctgtaaaaca gtcgcagaat
     6541 agccttttgt aacgaagcct actgattcta ccccggcacc taaatcaata atttgtgcag
     6601 cttctaaagc aagtggactt tgcacgtcat ttgtgatagc aacaacaggt aacccttgtt
     6661 tttgtatcat ttttactgct tcaacagtag aagcactttt cccgctttgt gacacagcaa
     6721 tcacagtatc gattgaagga gaaagttttc cataattgaa aaaataaaaa ggttcttgaa
     6781 tatcgacagt aagatcacca taagattcca atgctggctt cgctgctaaa caagcgttat
     6841 aagatgaacc cgttgctaag atcagtaaat tcactgtttt tttcatatgt tctcttgtcg
     6901 aaaaatcatt ctttttcaag atcgtactta gtacttcctt ttcttcatag atataatctt
     6961 gcatcgtaaa cattttctct gctccttttt taaaaaatcc ctatccacga accaaaaatt
     7021 ccaaagaaag ctagtccgat caagatcgtt aatggtttca cttctttctt caatagataa
     7081 aacactatgc caaatgctcc aagacataag atatttggaa cgatatcatc aaaaatactt
     7141 tgaacagtca cagcattttc tcctgaaccg atttttaaag gaatattgat ggtgatcatc
     7201 gtggcagtca ttgctccaac gaccatcagt ccaataatag acgcgcctaa tgtcaaactt
     7261 tccatcatac cgtttgcctg gatctttttc aaaaaacccg ttcctaagcg atatccccaa
     7321 cctgttgcta gatagcgaaa cagaatgtgc ggaatattga aaatcaaaat gaacaagata
     7381 gggcctaaaa tatttccttg catggctaaa gaggtaccca cacctgtggc aatcaatcgc
     7441 aaagttcccc aaaagaaaga atctcctaac cctgcaagag gtcccatcaa agatgttttg
     7501 atactatcga tcgcagtcac atcaaattca ggatcatcct tattttcttc ctccattgca
     7561 gccgtgattc ctaaaatcag tgtaacgata tgtggggtcg tgttaaaaaa ctctagatgc
     7621 cgtttcaaag ctgctgccat ttcttctttc gactgatata actttttcaa aatcggaatc
     7681 attgcataaa cataagccat attcatctga cgttcataat tccaagaaaa ctccatttga
     7741 aaagaacgcc aaaatacgtg atttagctct tttttcgtta ttttgctgtt tttgatctca
     7801 gaattcttca tcttcttcta cctcccctgc agttgtttga agtggtagtt gacggacatt
     7861 cataatattg actaccacaa ctgctaaaat cgcaccaaaa atagctactc cggtcacagg
     7921 aatctctaag taagaagcta tagcaaaacc taagaaaaaa tatggtgcaa cttgtttgtt
     7981 gatcagcaaa cgagcaagca tcgcaaaacc aagagctgga atcaaccctg tagctactga
     8041 caaccccttt tgaacgaagt caggaatcat atttaataga tttccgatcg tattgcttcc
     8101 caccatataa gaaaccatta ccacaacacc aagcatcaag gaaagaccga taccggcaag
     8161 caagtgcata tgctcgactc ctttataatt tccttcttct gcataattgt ctgctttatg
     8221 gttcataatt ggaatcaaaa gccctaagta aatatttttc aatatcaatg tcaacgtagc
     8281 aatcggcaaa cctagtaaaa gcgcggtttc tgtaccggct ccagctgtaa tagcaaaagc
     8341 cgtccctaaa atcccaccgg tcacaacatc aggtggaatc gaagcaccta ctgagaacga
     8401 tccgataaat gctaactcta aagttgcgcc cataatgatc ccagttttta catcacctaa
     8461 cacgattcct gtaaacagcc ccgtcacaat cggacgagaa agcaacgatg tccctagtgc
     8521 atattctgat tgtgcgacaa atgcaacaat tcctaataag actgcttgta caatcattga
     8581 ggttcttcct ttcttacaaa acttctctta catccacttt tttatctgct ggtacttgac
     8641 ggatttctac ttctatttgt ttgtcaatta atcgtttcaa gcgttcctcc tcttctggta
     8701 aaatatttac cgcttttccg atgctgcgag taccaggttt tgccttgatc cctcctaagt
     8761 tgatggtttt gatttctgga tacgcttctg ccagcttata tgcatcctcg atcgattcca
     8821 ctacgatgaa taacttatat ttatctgtca ccccgctttt caacgcggca atcgagtcat
     8881 caattgattt gatcactagt ttgactcctt gtggcttggc taatttgatc gttgtctttc
     8941 gtatctcatt cgtaggtaca tcatcattag caatcaaaat acagtctgcg cctattgttt
     9001 gtgtccatga aaaagctact tgtccgtgta ataaccgatg gtcgactctt gtcaatacaa
     9061 tcatttttct tcctcctaaa attcgtcttc ttctattttc tgattgagcc gctcgttaca
     9121 aaactggatc attttttttg aatcagttag cacctgctgg atcaaaacag ttgtctctac
     9181 ttcagcgtcc aaccgagata ctagctcaat caatagtggc aaattcaatc cggcaacgag
     9241 atagacatga tcccgattga tatattgtaa gaattcgttg ttgacacttc cgccaaaaag
     9301 atccgtcaga acaatgattt ctttatcttt gtacctcata aaaagctgcc ttactgtttc
     9361 cgctaaatca aaattctcat cagtatagca atcgaataca gtaatagaat gatttttccc
     9421 atagatcagc tctaatgaat ctaatatccc tgaagcaaat tttccatggg aagctaagat
     9481 aatttctcta tccacttttt catctcctct tttcacttac acatactaaa aaagcaagtt
     9541 gcatgccaaa attagtggca acaacttgct tatgttcaaa attcttcttc tatcgagaga
     9601 aactctgtat cttcactcaa aatatcatag atataggcaa tctcagaatt ggggatcatg
     9661 acactataat gctcttcaat gacactaaac gcagatttaa tagcagataa cccttctttt
     9721 tgacactgct tccatttctc taatccttga taggtttcga ttggcatttg tcggatcaat
     9781 cgttcaatga gacagctcac atggacatac aacgctaatt ttttgggatt gctgatgctt
     9841 cgattcaact gcacctctaa ttctctcata aaatgatcga tttctcccat caccttctct
     9901 gtatctagaa tagtcacgga atccagtact ttttccaatg aaaaattacg aatgatgcgc
     9961 tgattgaata tttccttctc tgaagcattc atagtagtgt ccaaccattc agctagtaca
    10021 gacatctttt cccccgagat gaggtcttct aatgaaagat agtcgatatt ggtgatatca
    10081 ggatctgtgg tgccaatcat tcctacgact tcgtatatgg aaaataatgg gtcactgttt
    10141 ttcttatctt ttaattgctg atattcatat gggatgattt tcaactgaca agccgtaggt
    10201 aagctttttt ctagcagttc acttaagtgg accgccgtac cgattccagt gaagcaagta
    10261 gtaatgagtg ctttgggttt gttttcctct ggatagagca gtcgccattc tggctggatc
    10321 tgtcgaacag tctcttctgc tatctcttct aatgatacat ttttttgcag gcattcccct
    10381 actgtgattg ctaaaggtgt cgtcacatta ttcaaaatca aaagcggtac ggataactgt
    10441 tttttaaaaa actgatggat ttctttcaga gatcccatgt ctactaatat gatcaatcca
    10501 tttgaaacat cgctgttttc gcaataatca aggatttctg aagcaatctg ctgtggtgtg
    10561 acatctagtg gcatatcaaa tgattcaaat agatcctctt gtaataaacg atttactaca
    10621 tttgccatac tgctagcagt tgcataaccg tgagcagcaa tcatcgcttt tggcagtcgt
    10681 ttttcttttg accatttcgc tttttttaga tagatcgtca gtaaaatccg atccatactt
    10741 gtgtcttcaa tatctaattt gatcttactt aattccagaa ttcgttggac atagtgataa
    10801 ctagatggat aagactgaga aacttgttgt tctaattctt tgattagttg gataatatca
    10861 tgttcttccg gtaaccattt gatagtgctt cgctgataga gatagtaact caaggcatag
    10921 acactgtttc catcgaattt gatttgatag gcagtttcca tttgtcgaaa ggtctctcgg
    10981 atagattgtg tccaataaac cagcagctcg tgtttttctt gtctactggt ctcaaacagc
    11041 aaaaaatcaa ataattgaat cacttcttgc tttaattttc cttcgcaaga atatagcaga
    11101 cagttatttt tcttgaaatc aattaggatt tgctcaaaac ttttaacgat tcgctgctgt
    11161 tcgggctgtt gcttctccat taattgctct aaggtctggt cttgcgtaat atagattcct
    11221 tgctgtgctg tatttttagt aataggctgc gcaagtagtt ctttttgcaa atgatggatc
    11281 gtgacatgga tttcaggctg ttcacgctgt tcagcatatg cttttgctgc tgaaactttg
    11341 acgatatttt tcagctctcc tatgtttccg gataatttct gacttgttaa aagtgacaaa
    11401 gctggtccac tgactgacaa agggctcccc atttttcttt gttcttcgag aaaaaaagag
    11461 taaatcagtt ctagccgttc attacgacta cgctgattaa gtgcaggtag ttcgattcgg
    11521 ataggtattc ttcgaatgaa agttgttaag aaatgactag taatctcttc agtcgtagca
    11581 aaaaagagac gtgttttgat ccggcgtgac aaattaggtt ctcccatacg ataaatcaca
    11641 ccttgatcaa gataggtgaa cagtttttcc tgaccttctg gtgttaagcg atgtacctcg
    11701 tctaaaaaca agatcccacc atctgctgct tcaaaagccc cttttttatt ttcttctgca
    11761 ccagtgtacg ctcccttaac atgtccaaac aaattgctag tcaaaagttc aggattatct
    11821 gcatattgtg cacaattaaa agtgatgaat ggtgcagaat cttctagcag atcatgaagc
    11881 agacaatact gatagaccaa attaactaaa taacttttac ctgtgccact ttcgcctgta
    11941 atcaaaagtg gtaatccgcc atctggatag taaagagctg ttttgatttg ttcgatactt
    12001 tctttcaaac tggcgtcgta tccgatcata aaagagaata agtcttttgg ttgcgcgaaa
    12061 aaaggctgtt cctctttgag atcatcgata gaaaaataaa aattcgtgcg taacttaaaa
    12121 aattgttttt caaaagcttc tttgtggaaa taaatcactg gtcgagaatt gattttgact
    12181 agtagttgct catcatttaa ctggttcaaa taatgactga ctgtgtttct ttttacccca
    12241 aaatgatcag ctaacctttg tgctgtaaag acctcgttca tatgttctaa atcgaaaaag
    12301 gctgtctggt tctctaagta acataaaatt tccttcttca acaacttatt cacccctcct
    12361 ccatcataga aaacggattc attttgtcaa taaaatcttt aataaagaat tgaaaaatat
    12421 atcttattat agacagcagc ctacttatcc cttataattt ttattctaaa aaacgtacag
    12481 ttccttccag tttttatact ggaatttcaa catatgaaag aataaggagg ctatgactat
    12541 tcaatgaaat cggataaata taaaaacagg aaacatactt cctggaaaac ttttcgagaa
    12601 atcatcggat cttatctcgc tgtagttgta tttttgcctt tgctttatat ggctatgaca
    12661 gaacaaactc gttttattgg cgtagatacg caatatatag ccgaaacagg atttttattc
    12721 atctttatct tgatttttgc gttctataaa tccttccata caaaataaga ttcacagatt
    12781 tcaactttcc tgtctgtttt tagctattac aaatccttat atgtggtata acaacaattt
    12841 attggcataa tgaggaaaaa cgagttatga tgctttcaga gagtaaagag gtactgttgt
    12901 caccatcctt tttattgatt acgtacacca ctcttagttt tttcccattg attacgaaaa
    12961 caaggtagaa acattgatgt tcctaccttg ttttcttttt ttattttcag atcaaaatct
    13021 caagtagctt ttgacttgat gtcgggaggt tgtcttcgtt ccatcaaacc tgatagaatc
    13081 tgcgccaaac catagttttc tccactatta tttattcctc taagatagta cccacaataa
    13141 acgaataaat atttttctca ttttcatact ccttctttga aaatcaatta gatttctctc
    13201 aacccacttt tttacaaaaa ttacgtagca aaaaag
//
LOCUS       DANKAY010000069        13127 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000069 DANKAY010000000
VERSION     DANKAY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            94..1833
                     /gene="yfcC"
                     /locus_tag="PWY98_001991"
     CDS             94..1833
                     /gene="yfcC"
                     /locus_tag="PWY98_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2037734.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1990..3174
                     /gene="iadA"
                     /locus_tag="PWY98_001992"
     CDS             1990..3174
                     /gene="iadA"
                     /locus_tag="PWY98_001992"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2037735.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3285..3488
                     /locus_tag="PWY98_001993"
     CDS             3285..3488
                     /locus_tag="PWY98_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2037736.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3589..4251)
                     /locus_tag="PWY98_001994"
     CDS             complement(3589..4251)
                     /locus_tag="PWY98_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037737.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4264..5766)
                     /locus_tag="PWY98_001995"
     CDS             complement(4264..5766)
                     /locus_tag="PWY98_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2037738.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6314..7993
                     /locus_tag="PWY98_001996"
     CDS             6314..7993
                     /locus_tag="PWY98_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2037739.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8120..8599
                     /locus_tag="PWY98_001997"
     CDS             8120..8599
                     /locus_tag="PWY98_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2037740.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8818..9498
                     /locus_tag="PWY98_001998"
     CDS             8818..9498
                     /locus_tag="PWY98_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2037741.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9506..10837
                     /locus_tag="PWY98_001999"
     CDS             9506..10837
                     /locus_tag="PWY98_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2037742.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10852..11814
                     /locus_tag="PWY98_002000"
     CDS             10852..11814
                     /locus_tag="PWY98_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2037743.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11925..12263
                     /locus_tag="PWY98_002001"
     CDS             11925..12263
                     /locus_tag="PWY98_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2037744.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12342..13049
                     /locus_tag="PWY98_002002"
     CDS             12342..13049
                     /locus_tag="PWY98_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2037745.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4167 a         2320 c         2881 g         3759 t
ORIGIN      
        1 ttcatgtgga aatgtcattt ttactgtata attcatttga ttatccattt tttattcatc
       61 aaaattactg aaaaaagaaa ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa
      121 ccgaaacgcg acttcactaa attatcgaca ccccatacgt atgtcattat ttttggtgta
      181 gtcatctttg catggatact gacttttgtc gttccagcag gaaagttcag cacgcaagat
      241 attgaataca aagatgcaaa tggcgaaaca agtactcgta ctgttttacg tcaagattcc
      301 ttccgttatg cttacgagct ggataagtcg tatgtgtttg atcaattaga agaactgcaa
      361 gatcatccag cagaacgtga aaaactggat gttccggaaa aaggattgga aaaagtcatt
      421 gctgacggag aaaaaaatct tacgcaggaa aaactagatg aaatctcctt gacagatgat
      481 gtgctttacg atcagtacgg cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca
      541 gccaaaatat ggggaacaga tgattttggc ggctttggtt tcttaaattt tgtttttgaa
      601 ggactagtat ctggagataa gtatggatct gctgtaggga tcgctgcatt gatccttgtt
      661 gttggaggtg cttttggtat tattatgcgt accggagcga tcgacgcagg tatctatgcg
      721 tttatcagta aaactaaggg actggagcgt ttagctttac cgctattatt ctttgctttt
      781 tcattcggtg gcgctacatt cgggatggca gaagaggtaa ttccattctc aatggtgatg
      841 gttccatttg tcattgcgtt aggatatgac tcgatcgtag cagtaaccgt gacttatgtc
      901 gcatctcaag tcggaaatgc gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa
      961 gggattgctg gaatccccgt gttgtctggt gcgacattcc gattgattat gtgggtagtt
     1021 gttactgcat tagccgctgg ttatatgatg atatatgcag aaaaaatccg taaaaaacca
     1081 gaaagttcgt tgacttacaa atcagatgat tatttccgtt ctcatatcaa aaagacatct
     1141 gatgaaaata aaaaattcat gcttgggcat aaattgatct tattagagat gttagttgtc
     1201 cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt acattccaga aatcgcttct
     1261 caattctttg tgatgggatt agttgctgga atcattgctg ttcttttcaa attagatgga
     1321 atgaaagtca atgacatcgc ttcttctttc caatctggtg cagctgattt agctggtaca
     1381 gcaatcgtag taggtatggc caaagggatt cttcttgtct tgggaggatc tgatgcatct
     1441 gttccttcag ctttgaatac gattttgcat gggatcggga cggcgttgac cggtgttcca
     1501 gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt tgttcaactt ggttgttaca
     1561 tcaaattcag gacaagcagc tttgactatg ccaatcatgg ctccactggc agatttagta
     1621 ggtgtatcca gacaagtagc agttcttgct tatcagcttg gttcaggttt tatggacgct
     1681 tttacacccg tatcagccag cttgattggt gtgctaggtg ttgcacggat cgaatgggca
     1741 aaatgggcaa gattccaaat caagatgcaa ggattcctgt ttgttttagg aactattttc
     1801 atcatgattg ccattgcaat aggtctgcag tagtactgga gacctgtaaa taaaaaagta
     1861 atccaaacag tagataagga ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa
     1921 agatcctatc gtttggatta ttttttaaga tcctatcatc agttatactt aaagaaagag
     1981 gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg caccagatta cttaggaaaa
     2041 atggatgtac tgattgccgg tgggaagatt ttggctgtag aagaacagct agaaggcggc
     2101 tacgaaggag tggaagtaga agaactttca ggtgagggaa aagtactaac cccgggtttc
     2161 attgactgtc atttccatat tttaggtgga ggaggagaag gcggttatca aaatcgaacg
     2221 ccagaagtca cattgagcca attgactaca gctggtgtaa cgacagttgt cggttgtttg
     2281 ggaacagacg gagaaggtag agatatgact gcgttgatca gcaaagcgaa aggtctggaa
     2341 gcagaaggaa tctccactta tgtatacgaa ggttcgtacc gattgccggt aaaaacagta
     2401 acagattcta tcatcaaaga ctttttgaca attgataaaa ttatcggaat cggagaaatc
     2461 gcagtatccg atcaccgttc ttctcagcca agttttgaag agttcgctcg tgcagtagca
     2521 gatgctcgtg taggaggcat gttatccgga aaagctggga tcatcaatgt ccacttaggc
     2581 ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag aagaaacaga aattccgatc
     2641 acacaatttt tgcctactca tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca
     2701 tttgccaaac gtggtggcac gattgacttc actgctagcg aagatcctga tttttgggag
     2761 aagacagatg gtgaagttcg cttcagcaaa gcactgaaac gattgatcga ggaagatgtt
     2821 tctttagata atttcacgat gacctcagat ggacaaggaa gcttgcctta ttttgatgaa
     2881 aacaatcatt tcctagggtt aggagtagga agtgccaagg cattgctagt agggatcaaa
     2941 gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct tacgagctat aaccagtaat
     3001 ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat
     3061 ctgtgtatct tggataaaga aacgcttgat attgatacag ttatcgctaa aggagaaata
     3121 atggtccaag aaaaagaagt caaagtatgg gggactttcg aaaaaagttt ttaatcaaca
     3181 gctctaacta tcttttttag aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt
     3241 accctgaagg tagatcaata aacaaactaa gaggaggaag cacaatggca gatttaaaag
     3301 gacgttctaa agatatgaaa gataaagtag tcggtgaagg aaaagaagca tacggcaaag
     3361 caacagacga caagagcaaa gaaacagaag ggaaagcaca atcactaggt gctgacatca
     3421 aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa
     3481 aaaaataaaa accacttatt actaattgaa tcaagcaaat aaaaaatccg aaccatatct
     3541 gcgagacaga tatggttcgg attttttatt tgatcattat cctgtttttt aaacttcgat
     3601 tttttgaaat tttttatttt ttagttcaaa gacttcatca cagctttttg ctacttcaat
     3661 ctcatgagtc acgatgataa tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt
     3721 gacgatatct tgtgaagttg tttcatctag atttccagta ggctcatccg ctacgattag
     3781 ttgatgttca cagcagatag cacgaacgat agcgactcgc tgctgctgac ctcccgaaag
     3841 tttggtcacc tgtttttttg ctaatatttc atcaattcct acacgagcca gtcgatctaa
     3901 agcaacttgt cgataattct cttgcttggt cttagtgatt tccattgctg ctatgacgtt
     3961 gtctagagcc gataaataag gaagcaagtt gtaagcttgg aaaataatcg agacatcatt
     4021 tttacgataa cttcttaacc cgattttgtt cagtgaacga tcttgatagt atacctttcc
     4081 ttcttttggt gcatctagtc cagaaataag cgaaagaaaa gtcgtttttc cagaaccact
     4141 tgctcctaaa atagcgtaca tttttccagc ttcgaaattc agttctacat ctttataaag
     4201 cgcatcttgc ggatcttgat accaaaatcc caattgttct gtccgtaaca tcaaattcct
     4261 cctttagttt aataggattt ttcttgggtt taatcttaat atccctatag aagctaaaaa
     4321 gacagaaaac agactgattc caaatgctac tccggctaat agtcccaatt gtgcaggttt
     4381 gacattgatt tccaattcgc tgacttgatc agaaacagca aatggattat tcgtggttcc
     4441 ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta cctggcattt gtccaccgcc
     4501 aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac
     4561 ggcattcgcg acgacgtttc cgctaagcga agcaactcct aatgcaagaa tcatacaaat
     4621 agccacttcg gtaaacaatt ggagaatgat tttacttctg gattctccta aagatagcaa
     4681 gacgcctaac tcatgccgac gctcccgaat ggtaatcata atgatcagag taagtatgat
     4741 aataccggct acagccacta gtaaaacaac atttttagaa aaacttgcca cgttgtttaa
     4801 aggttccaac attgactgat acatggaatc atttgattgt aagctaaatg tatctgtgtc
     4861 gatcagcttc tcagcttctt tgagaaactc atccatttta tctggatcag atagagtgta
     4921 aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt agttcatttg caaaagtgta
     4981 agaagtgtaa agtgtattcg acggattcat gaagttgaat ttcataccca tactgccgct
     5041 tgtttctctg gattcataaa ttcctttgat cgtcatttca taagtggtat cttcatcttc
     5101 agtactcgtg attgtgaaag tatctccgac tgccaggtca tttgtttctg ctagagtaga
     5161 atcaatgact acattgtttg tgcctttgtc gctgtcatct attccttcac catccgtgat
     5221 ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt gctgacacac cactgatttg
     5281 aaagtctgct tgagtcattt gcggattccc gcccattcct ccagcaggtt caccgcctgc
     5341 atcagtagtt tctgaactgt cttcacttga atcagaacta gtgatggcag ttattccatc
     5401 ttttgccata gctgaggcgg aactttcaaa ggaataactc tttacattat ctaattcagc
     5461 tattttttcc gcgtccgtta aggaaactgg tgtaagagaa aaactgcctg gatctggacg
     5521 ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg
     5581 attggcactt aaagtcactg ttgctccaac ttctttttgt gcttgattcg ctgcttcttc
     5641 tgcagcgctt cgaatcgtga ggccagctag cacaaaaata agaatggctg tgaacacggc
     5701 aaacaataac aatgaacgtc cccattttac ttttgtactt ttccatgcac gtttcataaa
     5761 attcatattt tgcatccctc ctagtagatg catttgataa tagacaacct atcttaaagc
     5821 tagcttaaat cttatgctgt ttgcacacca tcaacatgag gttctgtata tataaaaagt
     5881 cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag
     5941 aagaaatgaa agaggaatag caaaagaaat cctagatgct acagagggat ttttacgtgc
     6001 atagttgttc taacaattgc tgatactagc gatttagttt atttgttgta taataatggg
     6061 gaacagctga atacttatat aagtattctg gctatagcgt ttcgcaaaag atccattttg
     6121 ttaatgaagt atatccaaaa taaaatgccc atccaactgt cagaacgcgg atgtgctggt
     6181 aattttcctg ctgattctcc ataagttgag aaatagtgat tttgcatatg cttcattgga
     6241 atggctcata tatttagata aagaagaaaa tcgacatctt taaacttgga tgtcataaga
     6301 agaggtgaag tttgtgagta ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg
     6361 aaaaaacatg tatgtcgcag aagtgggcga ggatattttt attttagatt gtgggctaaa
     6421 atatcctgaa aatgagttgt tggggatcga cgtcgtaatc cctgatttta cgtatttaga
     6481 agaaaacatt gatcgtgtag ctggtgtctt tttaacacat ggacatgcag atgcgatcgg
     6541 ggcacttcct tacttgttgt caaaaatttc tgtaccagta ttcgggacag agttgacgat
     6601 cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc aaaggattta aggatttcca
     6661 tgtggtagat gagcatacag aaatagactt tggtgaaaca gtagtgagct ttttccgtac
     6721 cactcatacg ataccagatt cagtggggat caatctgaaa acaaaagaag gtagtatcgt
     6781 ttatacggga gattttaaat ttgaccaaac ggctatccca atgtatcaaa cagattatgc
     6841 acgattggcg gaaatcggca aagaaggagt cttagcctta ctaagtgaat cggcaaatgc
     6901 agaaaatcct cagcctgtag cttctgaagc ccaaatagca gatgaagtat ttgatacgat
     6961 caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca
     7021 acaagtattg gatgcagcag aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt
     7081 cgaacgaatt attcgcacag cgatgcgttt agaaaaactt caattaccaa gtgaagatct
     7141 gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa gaactgatca ttttagaaac
     7201 tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa
     7261 tgtcaagatt gaagaaggag atttggttta tatcactact acgccaagca tcgcgatgga
     7321 aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct ggtggaacag tgaagttgat
     7381 ctctgagaat atgcgcgttt ctggccatgc caatcctaat gacttgcaat taatgatcaa
     7441 tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag tatcgtgaat tagctgctca
     7501 tgcagatttg gctcatgcag tagggatgcc atacaaaaat atctatatta ctggacgcgg
     7561 agacgtgctg gaatataaga acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa
     7621 tgtaatgatt gatggattag gcataggtga tatcggaaac attgttttac gtgatcgaaa
     7681 aatcttgtca gaagacggga ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa
     7741 aatcatttca aaacctcaaa ttacttcacg gggattcgtt tacgtaaaag ctagccgtga
     7801 tctgatgaaa gaaagcagca atatgattga aacaatcgtt gagaaacatt tagaaagcaa
     7861 tgattttgag tggagcaaat taaagcaaga catccgagat caattaggcc gttacttatt
     7921 tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt atggaagcta ctcaacgtcg
     7981 aaataaaaaa taaacaaaaa gagtaagatg aacctctcta tagagtgctc gtcttgctct
     8041 ttttttgcga aaaacaaata atttttgaat acagcatccc tcgctttata ataaacgata
     8101 gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc gaacgttatt tagaagaaca
     8161 aaagatcagc tatcaaccac ttgattttag tcatgtggta gaagaatctt tcacagaaga
     8221 actacaaaaa agagggattg atccagcttt gatatgtaaa actctcgtag tgaaaggaaa
     8281 caaaactggg gtgatcatcg cattgattcc tttgaacgat cacttagatt acaaaaaaac
     8341 gagacaagtg acgaaagatc gtaaagttgg ttttcctgga atggattttg tattaatgca
     8401 tacaggatat ccccacggag caaacacccc tattggaata aaactcgcac atcctgatta
     8461 ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga
     8521 aatcgggaga agccttcgga taaaagtagc cgatttagaa aagatcgtcc atcctattta
     8581 cgctgattta attagttaag ggacgagaag agaagctaag cagagcagag acctcaaact
     8641 tgagattata tctgcttggt acttggtgca tcctgtatta tctgttacaa ttgctttgta
     8701 catactgtac tatcaatcca atgaaaatat atttagcagc tatatttttt cattgatgtg
     8761 caagtgatga aatcgttttt aataaaaaag atacagagaa aacgcgaggt gtaaaatatg
     8821 aaaattacgt atcatggaca ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg
     8881 atgattgacc cgtttattac aggaaatcca ttgacagact taactgcaga agaagtaaag
     8941 acagattggt tgctgattac tcatggtcat agtgaccaca taggagatat gattcctatt
     9001 gcaaaagaaa atcaagccac aattatcagt attgttgaga ttgctgatta tgcgaacagc
     9061 agaggagtag attcattcgg tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg
     9121 gtaaaattcg tacatgcaca gcatagttca agctatgaag tagacggtat cgttcagtat
     9181 atgggcgagc ctagcggaat tattattcaa gcagaaggaa aaacgattta tcatgcaggg
     9241 gatactgctt atttcagtga tctaggattg cttgcagaag aattcgatat tgatgtcgct
     9301 ttcttgccga ttggcgataa ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa
     9361 acaattcgtg ccaagaaagt agttccaatc cattacaata ctttccctgt cattgaacaa
     9421 gatcccgaat cttttgtaga gttactggaa gatagtgaag gaaaaataat gagcgtctcg
     9481 gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat gatttgattt tagaatatat
     9541 tgaaagttta cctgttggca atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt
     9601 cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac
     9661 aattcaacga gtagggacca tccgtatcga acgcaaatta aaaaaacata ttgaacgttt
     9721 gacatttggc gaggtcgtca ggatcattga aggggacgtt ttaggcgggg cggcaggatt
     9781 agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta
     9841 tattacgcca ggatcactaa tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt
     9901 ggaagatggc gccgcagtat tgatcacagg aggattcaat acgactgctg agattgcaaa
     9961 gctagctgat gatcttgaaa tgccgatctt acgcacctct tatgatacat ttacagtcgc
    10021 aaccatgatc aaccgagcgt taagcgacca attgatcaaa aaagatatca tgcttgtcag
    10081 tgatatctat atgccagctg aaaagactca ttatttgcag cagacagata cgatttctga
    10141 ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt ccagtcgtca accgccatca
    10201 tcgtttgatg gggattgtta cggcaaaaga tgttttaggg aaatcgccaa accagatcat
    10261 cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa acaatgagta ttgcctctgt
    10321 tagccatcaa atgatctggg atgggctaga agtcatgccg gtcgtttccg atgacttgac
    10381 attggaaggc ttgattaccc ggcaagatgt aatgaaagcg atgcagcttg tccagcgaca
    10441 accgcagatt gcagatacga tttcggatca gatatctgga aatatccata cgattgatac
    10501 tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa ttcgtagtag caccacaaat
    10561 ggtcaatggc gttggaacca tttcttttgg cgtacttagc gaaatcattt ctgatgtcgc
    10621 acaaaaaaca atggtgatga accaaaaacg aaatatcttg attgaacaag tgaacctaca
    10681 ttatttgcgg ctgatccaat tggaaagcga attagacatt cgtccacgtg tattggaaat
    10741 cggcagacgt tccgctaaac tggatatcga agtgtttatt gaaaacacca tcgtttccaa
    10801 agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa gcaggagtga aatgacagta
    10861 cagcaagaaa tagtagatac aataaaaaaa tacgataaaa tcattatcca caggcatttg
    10921 cgccctgatc ctgatgcatt aggatcacaa gtaggattag ccgaactatt acgggaaagt
    10981 ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg aagggcttcg ctttttagca
    11041 gaaatgcaag aagttgaaga tgtcttctac caaggagcgc tagtcatcgt gactgataca
    11101 gctaattcac ccagaatcag tgatcaaaga tatcagttgg cagaaaaact gatcaaaatc
    11161 gaccatcatc ccaatgatga cccttatgga gatcttgtgt gggtaaatac aaaagcaagt
    11221 agctgtagtg agatgattgc agaatttgct ttgatgtttc ctgaatcttt aaagatgaac
    11281 gttaaggctg cacggttact ttatgcagga atcgtcggag atacagggcg ctttttgtat
    11341 ccctccacga cgtcccgtac attagagatt gcttcgatat tgagaaatta tccatttgat
    11401 gcaagtgctt tgaacagaga gattgaacaa atgccgatga aagtcgctaa actttccggt
    11461 tatctttatc aaaatatcca ggtggatgaa aatggtgcag gaagaataat ccttccgcaa
    11521 gaaattcttg ataaatatgg aatcgatgat tcagagacag cttcgatcgt ttcacttcct
    11581 ggagtgattg acgaagtgct ggcttggggg atttttgttc agcagccttc agggtattac
    11641 cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat tggctaaacg ccatcatgga
    11701 ggaggccatc cattagcaag tggtgcgaat gcaaaagatt tagcagaagt agaggaaatc
    11761 tataaagaga tccaagatat atgccaatcg tataagagca gaaaaacaga atgatacttc
    11821 gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta tcttttcaag atcttctttt
    11881 tccgttacaa tagtgctcac taaagatttt aaaggagaaa gaagatgaca ttaccaaatt
    11941 gcccagaatg tggctcagag tacacatatg aagatcgagg cttgttgatt tgcccagaat
    12001 gcgggaacga atggaacaaa gaagaacaag aaactgtcat agaagacgga ttagtggtat
    12061 tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt gacagtgatt aaagacttga
    12121 aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa agtcaagaac atccgtttag
    12181 tagaaggcga tcataatatc gattgcaagg tagaaggatt tggaccgatg aaactgaaat
    12241 cggaatttgt gaaaaagaat taaagaatat tcaaggtctt ttcacattct tttcctacta
    12301 ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa aatgtatggt tatggaatgt
    12361 atggtttttt agatccgaca tttcttttgg tcattcttgg attagttatt tccggaattg
    12421 cgtcagcgta cgtcaatagt actttccgaa aatacgatca agtgagaagc aaaaacaatg
    12481 tgactggtac acaagcagca caatatatat tacaaagtca acagataaat aatgtcggtg
    12541 ttcaacaaat tgccggagat ctcacagata actacaattc aggtaataag atgttgagtt
    12601 tgtctcaagc aactgcacag tctacttcag ttgctgcgat aggtgtagca gctcatgaat
    12661 gcggacatgc ggtacaagat gcttcgggtt atgtcccatt gaagattcgt gcagcacttg
    12721 ttccagcagc taactttggt tcgatgattt cgttcccctt gattatggta ggtgtattgc
    12781 ttagctggaa ctctacgtta atcaatatcg ggattttcgc tttttcttta gcattacttt
    12841 tccaattggt gacattacca gtcgaattta acgcgtctcg gcgagcaatc caaattttga
    12901 gtgaaggcgg actactgacg gaagaagaag taccgatggc aagacacgta ttatttgcag
    12961 cagcactgac ttatgttgca gcagcgcttt cgacattcct gcagctgttg cgcttgatct
    13021 tattgttcgg cggcaaccga agagattaaa ataaagaagt tgtgaaatca gcgctttgat
    13081 ctgagctaat accgagagtt agctgattga ataccattta tttggaa
//
LOCUS       DANKAY010000070        13020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000070 DANKAY010000000
VERSION     DANKAY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            78..686
                     /locus_tag="PWY98_002003"
                     /pseudo
     CDS             78..686
                     /locus_tag="PWY98_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="PWY98_002004"
     CDS             706..1734
                     /locus_tag="PWY98_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2037746.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="PWY98_002005"
     CDS             1731..2489
                     /locus_tag="PWY98_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2037747.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="PWY98_002006"
     CDS             complement(2575..3315)
                     /locus_tag="PWY98_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2037748.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="PWY98_002007"
     CDS             3376..4629
                     /locus_tag="PWY98_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2037749.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4930..5178
                     /locus_tag="PWY98_002008"
                     /pseudo
     CDS             4930..5178
                     /locus_tag="PWY98_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="PWY98_002009"
     CDS             5300..5626
                     /locus_tag="PWY98_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2037750.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="PWY98_002010"
     CDS             5694..6407
                     /locus_tag="PWY98_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2037751.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /locus_tag="PWY98_002011"
                     /pseudo
     CDS             6717..7569
                     /locus_tag="PWY98_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7690..9555
                     /locus_tag="PWY98_002012"
     CDS             7690..9555
                     /locus_tag="PWY98_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2037752.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9603..11012
                     /locus_tag="PWY98_002013"
     CDS             9603..11012
                     /locus_tag="PWY98_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2037753.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11120..11821
                     /locus_tag="PWY98_002014"
     CDS             11120..11821
                     /locus_tag="PWY98_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2037754.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11980..12459
                     /locus_tag="PWY98_002015"
                     /pseudo
     CDS             11980..12459
                     /locus_tag="PWY98_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4366 a         2189 c         2753 g         3712 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
//
LOCUS       DANKAY010000071        13015 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000071 DANKAY010000000
VERSION     DANKAY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..1422)
                     /locus_tag="PWY98_002016"
     CDS             complement(<1..1422)
                     /locus_tag="PWY98_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2037755.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQ"
     gene            complement(1519..2019)
                     /locus_tag="PWY98_002017"
     CDS             complement(1519..2019)
                     /locus_tag="PWY98_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2037756.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(2032..2253)
                     /locus_tag="PWY98_002018"
     CDS             complement(2032..2253)
                     /locus_tag="PWY98_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037757.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            complement(2258..2395)
                     /locus_tag="PWY98_002019"
     CDS             complement(2258..2395)
                     /locus_tag="PWY98_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2037758.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(2392..3576)
                     /locus_tag="PWY98_002020"
     CDS             complement(2392..3576)
                     /locus_tag="PWY98_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2037759.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(3832..4086)
                     /locus_tag="PWY98_002021"
     CDS             complement(3832..4086)
                     /locus_tag="PWY98_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037760.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(4111..5253)
                     /locus_tag="PWY98_002022"
     CDS             complement(4111..5253)
                     /locus_tag="PWY98_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2037761.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(5313..6665)
                     /locus_tag="PWY98_002023"
     CDS             complement(5313..6665)
                     /locus_tag="PWY98_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2037762.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(6736..7080)
                     /locus_tag="PWY98_002024"
     CDS             complement(6736..7080)
                     /locus_tag="PWY98_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2037763.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(7090..7464)
                     /locus_tag="PWY98_002025"
     CDS             complement(7090..7464)
                     /locus_tag="PWY98_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2037764.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(7477..7791)
                     /locus_tag="PWY98_002026"
     CDS             complement(7477..7791)
                     /locus_tag="PWY98_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2037765.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(7905..11132)
                     /gene="fss3"
                     /locus_tag="PWY98_002027"
     CDS             complement(7905..11132)
                     /gene="fss3"
                     /locus_tag="PWY98_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2037766.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(11211..11873)
                     /locus_tag="PWY98_002028"
     CDS             complement(11211..11873)
                     /locus_tag="PWY98_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037767.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            12490..12720
                     /locus_tag="PWY98_002029"
     CDS             12490..12720
                     /locus_tag="PWY98_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037768.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            12726..>13015
                     /locus_tag="PWY98_002030"
     CDS             12726..>13015
                     /locus_tag="PWY98_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037769.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPER"
BASE COUNT         3681 a         2873 c         2131 g         4330 t
ORIGIN      
        1 ttgtatttct tttttcacag aaacaatatc ccaatttaat tttcttattt cgtttacaca
       61 tgtttcaatc tcatttttat actctgaaat caaatcagag ttatatccat ctagtaattc
      121 tagtatacta gtttgatttt ttgaaatttc ataaatttgt ttttgagagt atatactcac
      181 cttatcggaa atcattctca accgttctgt ttctttttca accagaccat tctcttgtgt
      241 aaattcataa atagaatgtc tatcctgtgt tcttattatt tgatattttg tccccttata
      301 gaaaatattc agtttcacaa ttgtatcctt caaaaatatt ccagagccat tatgttcaat
      361 ttgtgcaaag ttctgataat cactttgtat ttcagaaaat tgatcaagtt ctgcttcctt
      421 tccaaggaca tagaccaaga atcttgtaat acaagatttg cctgttcctc ttcctccaat
      481 aattgaagtt aaattatcgc tgaattcgac tacaacttct tttgtgctaa gagtggtccc
      541 agaaaagctt aatttttcca ataaaacttc attttcatta atggagaaat tagaaaaatc
      601 agaatgaact cgagtttcgc ctagcattaa agcatcgcga agtgatccca agtcaggttc
      661 gtccttcatt tttatatagg aatatcttgt gcctattccc caaagtccat gttttgaatt
      721 tcctttcgag tgtgggttat cagaaaagct aagaaatgtc atattatttt tttctgcctc
      781 aacaactgat ttataccaag attccaaagt atcttttcca actctaccat catatctggc
      841 attcacacta tcatagatag tgtcacgctt agatgaggga atcatttgtg agcgattttc
      901 aaaaaattct ttttgaaccg tctgtactgc atatatattt tctgattcaa atatctttcc
      961 tagatttcca aattttgctg aagaaattcc attaaactca tctatatgtg caggaataac
     1021 gagcaagccc tcgtgctcag caaaatcaat aacttctaaa actgatttca ttgagcaagc
     1081 tgcagcgctg gcccaatcct ctctttttat acccatcttg gttaagaaat cagagatatc
     1141 atttgtacat gcacttttat caaataggac tagtaaatga gttccatttt ctccacacgt
     1201 taattccact ccaggaaaaa aatctaagcc attttcgata gccactttct tatattcatc
     1261 aatattttcc cctgtattgt gatcagttaa tgcaacacaa tcgagtcctt tgtttaaaca
     1321 ttcttctacc cattccttag cagtaacctt tttatcctga aagcactgac tcgcagttga
     1381 atgaatatga agatcacatt tgtaccactt attgccaacc attagtaaca cccctatcaa
     1441 caaatatttt ctatttcagt atatcaaata gaaaagtaaa agtacgatag aaatttctgg
     1501 tcaattctaa caaatgattt acattagttc aaaaacccct cgactcgtaa ccaaataatt
     1561 cccgccaatc tccaagtctc gaccacacgc atcaaaatca atatacgtct tcaagtgttc
     1621 tggtacttcg cctaaatcac cagcttctac aatcaagtaa tacgctacat cactcatgtc
     1681 attacaatcg ggataataga tgatatcatc cacatgctct accatttcct cgaaagaatt
     1741 gaagaaggca tgttgaatct ctgaagcgac ctcaccaatt ggcgttcctt taagctcttc
     1801 cgctaaacca cacaaccgat taatttcctc aattggtgta tactcatcaa tctcaaaagg
     1861 cagttcataa tcatgaattg cgtactcttc atattcgtca tttaatccaa tccgttcttt
     1921 cacttcatca aaatcgacgg gtggggtaaa ccatgccccc accaactcac cttcattgta
     1981 ttttccaagg ttcgcaatat aaacacgtat ctgttccatt gtgattcccc ctcaggcacc
     2041 gacaattcgg ttaaacaaat tgagtaagac atccttcaca ccagaagcat taaagaccag
     2101 acctaccgca atcaaagcaa tcaccaagaa gccaatcagt ttggaaaact cgcgtttaaa
     2161 tcctaaaaac aaaccaatga ccacaatcgc tagtaagact aaagattgag cattggttaa
     2221 aaaccattga tacaaatttt gaccgaaatt catttcatca ctttcctttc ttttccattg
     2281 ctcggtccgc cgctgcactt gcatgcatca agcacatcac agtaacacca agcatactgc
     2341 caccaaacac caataaaata tctttcacaa tttccatcac gattcctctt ttcatataat
     2401 caaattttca attgccatac tttgttgctc caaaatcttt ttgtgccggt ctgatagtcg
     2461 agtattattt accatatcct gaatgtacgt tgtctgattc aaccgatcca actccttggc
     2521 catcttccac gtaggggcga cttgtcgtcc aagccagttc aacgttcgtt caatggtata
     2581 aggcttcggt tcagaagtta attgaatttc cttctgattc cgtccaatga agcgccccca
     2641 ttgttcattc aagggccagt tagttcgcct tttgccttcc accttatccg caaatcgcaa
     2701 gtaccggtta ataatggaaa atgtggtact ttcaatactg cgacctttta acaaatcttg
     2761 aattgcatga taagctcgat catttttcag tcggatttca aaacgatttt tcgtttcggt
     2821 ctggtctaaa ggaatcccta acttgatata ttgctcatag tctttttcgt acaaacagaa
     2881 atagacctca ctcttaagac tgccaatata caaagtattg cccataccat ctttatcatc
     2941 aggtttcaac agttccccgg aacgatagct tttgaaggta cgaaacaagg agatacattc
     3001 ttccttctga cattttttcg ttaaatcagg aatatctaac aggccaactc gatcattgat
     3061 tgccaggtct aagcgtttca tcacgccacc ggcttttaga caatcttcga aaaagtcata
     3121 ccagctacgc ttttgggcga gtaaaaaagt ttcaaactgc cgacaaccac gaccctttaa
     3181 ttctaaaagg acgcctttat cttcttcatg tgacaacatc accacaatat ctcccataac
     3241 atattgttct tgataggagt aaaaggcgta atcttcgtgg agcatataat caaatttcaa
     3301 atggagaatc tcttgaatga ttttcctcgc atccgttgtg ggaaagcgaa tccttacata
     3361 gtcaaacagt aacgttaaag gtaactcggg attgaaactt tccagttggt caaaaatttt
     3421 cctcatggtt tttgtcgatg cggttacgcc accattttct atctgattta agtacgggcg
     3481 actaagtcca caagtcgtag ctaatttatt ttgtgaaacg ccatattcat tacgccggtc
     3541 ttttaatagc cgataatcta aattcctttg tgccaagttc tcacctccaa atttaaggtg
     3601 ttaacttttt caaatcagtt aacatctaca caaattgctt attatccttg ataggacagc
     3661 cattccttat cgttgcttgc ttattttttg tgtttttgta cccccctgtt agatacgggg
     3721 ggtgagacat catggccggc taacgccgtc cataaacagg cacagcacca cggaggattt
     3781 ctgcttcgca aatcccccgc ggtcagtgcc tatcagtggt ttatttggcc gttactccgc
     3841 ggtaataccc tcaatttcca actctgcttt tatctcaaac aagtattggc ggatatcagg
     3901 ttctttagca aagtccgcaa ttcgttccaa ttcactgatg acttgattca gctccattcg
     3961 agcatccgtt tcctcatcaa ttacatacgg atcatcaggg actttcaccc cgtttttgcg
     4021 aatatcaaaa ttgagccggt acagttcttc ttccaattcc ggatctgact gaatcttgcc
     4081 aatcacccag tagattcccc aatcaactca tcataaatca tctgttttta cctcctttaa
     4141 tcttctagca atatcaaaaa tgaccggaac tgttacagaa tttccagcct gcttatacaa
     4201 ctgactatct gaattgactt gagaagctcg atcaaaagcc cagtccggaa agccctgaag
     4261 ccgccagcac tccctaggtg ttaactttcg aatttgatac tgatccgtga cgactgcttg
     4321 ttggtctcct gttaataagg tatgcacact ttgttttccc agacggcctc gacgacttgt
     4381 ggaattagga aaagccaggt tcacactgtt ccctggcttc acctcttgat attctgtttc
     4441 gtttttccga agcatgatct tcgttccgcc ttgtttcgtt gacaaagtag gcgctgttcc
     4501 agttatctca taaacccgat tgccttgttc aaaatttccc agtaacatac ccgctacaga
     4561 aattccatca tctaccggca atgcgatttt gattccttca tcaccggtca aggtaggcgc
     4621 tacgtcatct gacccataga cttgtccccc cattcctcga ttggaagggt tgatatttaa
     4681 taagtttctg atgttttgat cagatgttgg agacgatcca atgataggaa atacttgttc
     4741 ggtacatgcg tctcgagaat gtccgataag gaagatccgc tcacggtttt ggggaacgta
     4801 ggcctttgag ttaagcactt gccattccac atcatacccc aatccatcca gcgttcggag
     4861 gatcgtctcg aacgtagccc ctccttcgtg attgagtaat ccccggacgt tctcaaggaa
     4921 taaaaactta ggttggagaa tagcggcgaa ccgagcgatt tcaaagaata aagtacctcg
     4981 agtatcggaa aatccttgcc gctttcccgc aattgaaaaa gcttggcacg gaaatccacc
     5041 gcaaaggata tccactggtc cgagagattg aataaactcg tctgatacac ttgtgatgtc
     5101 atgcatttcc acctccttac ttgtatcatg gattgcctta taactctggc gagcaaaatc
     5161 atcaatttca caaaagccga tacactggtg acccgcttgt tccataccta agcaaaagcc
     5221 accaatccca gcgaataaat ctaaaaatgt cataaatcac ctcgtaaaaa aagcccagga
     5281 ttcttgacac tcctaggcta catactatta ttttatcctt ccgtattaac gtttaatcca
     5341 ccaatgtcgc taatttccct taagaaatca tatccttttg gtacaagtgg cgtataaaac
     5401 tcactaatca cactccctcc cgtatctaca tagcctcgac ctttgatacg cttcataaag
     5461 aattttttat caacctcgcc aaacatcatg gaatatccca gttcactcat acgacccaaa
     5521 gccacccaga aattaaattg atcgcgaatc ccgtctccta aatatttcgc atccggtctt
     5581 tgacaagcta gaatcagaaa gaaaccagat tgacgaccta acatcacgat ttgtttgagc
     5641 ttattcaaaa tcaccgcact ttccttcgtc gttaacattt ccatataaac cacgtattca
     5701 tcaaagatta aaaagtttgg tggaagtcca agaaacgcat aattctctcc tgttttgtag
     5761 ttgggcattt ctttcattgc cttgctacga gtcatcatgc gctcatagaa atcttccaca
     5821 caagcagaaa tttcctcctt ttgggaatag acatgaggca ttaccgtacc taaatcggct
     5881 aagtccgcat ttttgggatc aagaacaaaa agctcggcat cagattttaa taaagcctcg
     5941 atgatagtga gtaaaaagta agtcttccca ccacctgtgc cgccagcgat taacatatga
     6001 ggtaaagaat cataagccca tacttgattt ttcatcaatc gcaaagcccc actttctgcc
     6061 actacttcgt ctatcccaat tcgattggca attatatcat acagtaaagt gtactccaca
     6121 taggagtctt tgagttcctt ttctactagt tcacaatata gaccactttc taacttcttt
     6181 tccagcttta aaagctgatc ttgatatttt cccagtgaaa tttgaacgcg aatagaaagt
     6241 aaaccatcct tcatacggta atagattttg gggaagtaac tgattgtctc tttggttcga
     6301 ctactattga ggtctttgaa aaatccttca ctctgagttt gttttacttc gtaccaatga
     6361 ttttctagaa tcatccgggc tagcttttgt cgatgccaca aactgcgcca atgatcctga
     6421 aaaaagtgta tataaagcaa catcgtgctt cctgaaagaa cgagacttcc tagcaaatag
     6481 attaaatctt ttatagaacg aacgatttct atagtagaaa gtgatagctt agaaatatca
     6541 aattgtctaa gctcttgaaa ataaaagacg ctcatccaac cgatgaacag agcgagtagc
     6601 cagccaagaa caaagtgata aaccaagtgc tgatctcctg cccgtatacg atgccctttg
     6661 tacattttta tggtttccac ctccaatcga aaaaagcttc ataggatttc attgatccaa
     6721 tttcaagcag ttatgctagt catttactgc tatagggagt tgtttcgttt ccactagttg
     6781 ctttaaaaaa acttcaatgg catccggcat ttccacacca atttcttcca atacggtttc
     6841 ggcttctcga catagacttt catttacttc gataaccata aagaaggact cttcttcccc
     6901 taaccaattc agtacatcaa aatcttcttc ggcatcgtta cctacttcca gtaacccttg
     6961 atgcataatc agaaacattt tttcatcata ggttaatgct ttagtgagtt gttcctccgt
     7021 tagctcacct tttccatatt gatcgagaca atctaaaata tgttcaacat taatttccat
     7081 acgcttcacc tatttctttc cttcattttg gtttgtagaa tttccctgat ctggttgtcg
     7141 tttcaagact aaatcatcgg ccttaatgaa ccaattgaca gtagttcctt ctcggaaaac
     7201 ataattggcg accgtatcca tgattggatt gaccaactct acctctgcgt cataggcaaa
     7261 ctcgcgtaag ggtacatttg ctgggatact aacttggatc attcgacctt gcgcagagga
     7321 tttcaaatta taagtccgtt cttttacctc gttactaacc gttccatcct cattttgctt
     7381 ccgtacttct cggcgtaatg cggaaaatcg taatttccca aacgttagtc ctgaatctac
     7441 aacaatacct tctgttaatc tcatcttctt ttcctcctat gctttcacta aatcatcgac
     7501 atacaacacg tagtcggtaa acccacgatt ctcaatctca tagcctttgg caaccaaatg
     7561 gggattcacg gcttttagtg gttgatcttg atcaaaatcc ttttcgccgg cttccgcagc
     7621 taccaccact tcaatatcat cggctcgttg cttactggaa tacaaatgat aactacgacc
     7681 aattactgta gtattccgcc catatccttc agttaaaact tcgccttcgc cggcatacct
     7741 tatctttcca aaggtttcgg ccatatctgg cacaacaaat ttcaattcca tcttcttttc
     7801 ctcttttcat ttttagtttt ggatacaaaa atagcgcgac cacttttttt aggaggcggt
     7861 cgcgcacgtt ggtttttcct atttagttgt ttcatcacat caccttagtt tcggcgattc
     7921 cagaaataat acccagcagt cgcaaagatc aaggtaaacc caataaacag cagaacatta
     7981 gaatttttct cccctgtttg tgggaatgtc tttactgtag gactagattc ttgagagtca
     8041 cttggaacag ttggtatttc cggttgttct ggtgcacttg gtgttgtcgg aactttttta
     8101 ggtgttaaag tttgagtttt ttcttttaga tcctcattgt gtttcccatt gatgtttccg
     8161 tctttgccat agttaatttc tttgaaggta aaacttgtac cttctggata tttacttgtg
     8221 tccacttttt ccgcaagtac ggttttagtg aattctttgt cattcacttc ataatcaatt
     8281 ttgccagatt tccaaatttc tttggtcgta ttatccggaa gtaatgcata cagaattgtt
     8341 tcaaaagctt cttttgagcc atccagtaca tcatgggtaa tcgacacatc atcgaacata
     8401 tccaccacat ccccatgagt aaaggtttgc gaaccatctt ctaggtgggc ttttgtttgg
     8461 atggaaacat ggcgttccac cgtacaattg acagtttggg cttgattgtt caagctagca
     8521 tctttcgcta ctggatcatc acctgcttca tagggtttct tgttttcata cacatagttg
     8581 aataagacga tggttttatc caaaacttgt tctgcggtta atttatgcaa aattttccaa
     8641 gttcctgttt tctctttatt cgccaacgtt gcggttgttt cggcaattac cactggtttg
     8701 gctttttcat ctttttcttg ggcagctttt gtcgcttcca catcaatggc ttgcgctacc
     8761 acataccagt cttctttgat ttcatgcaag ttatagctca atttatcaac tagctcggta
     8821 gaatcaaggt ctgtcaattc tttgcctccg tctttccaag tcccaagaga agttaagcta
     8881 tcttcttctt caggtaaatc atagagcatc aaacgattca agctaacaga aaatgcttta
     8941 ttcgtcagtt tctcgtaagg aacggtcacc gttttaattg gttgtttttg tcctttttcg
     9001 gtaatcgtaa aaatgtattc actcttagtg taatccgctt tattttcttt aaaagtagaa
     9061 cggatttcta atggcgtaat tttttgaaat ccttctggag cctctacttc ttcaagtaaa
     9121 taatccccat aaggaagatt ttcaaacttg ccatagccat caaaacctag ttgctcgtta
     9181 taagctgtaa ttgcttcatc ttcggcatct gtaatctcac tggttccttc caatggcgat
     9241 actttaaagg tcagatcatt gaagccagtt tcggcagtcc cagcagctga tccagcaaat
     9301 ttaaagaaat caaagccaaa gcgaatgact tgttcttttg ccgtaacatc tcgggtaatc
     9361 acggcatttt tttcgttatc atccaccttt ttgatggaaa cagggtactt tgtttcatcc
     9421 aaggtatatc cttcaggtgc tttggtttct tgccagtaat actcattaat cgctaagtgt
     9481 ttaacagcaa cttgattctt ttcatctaaa gtcaaagtca ccgtttcatc agaagctttc
     9541 gttcccttca ctaactctgg tttaaaagcc tcactccatt tgaccgcttg accatctttt
     9601 gcagtaaaga gagtatattc agctccttta aactcagctt taccttgagt ttcattaccg
     9661 gtatctttgt cttcttttgt caaagtggtt tcaccagtga tttcttggtt ttgccctttt
     9721 acgttactgg taacaagagc cacggtttga ttgacatagt tcaattcgac ttttactggt
     9781 ttaaaggtat tcacaaagcc attactagct ttggtttcgg tcacataata ggttcccaat
     9841 tccaacgcat tggcaatctc ttttggtgtt tccgcatgac ctttttcatc cgtggtcatt
     9901 tcttggacaa tttcaccggt tggactgtct ttgcgaatgg caaacgtatt tccggctaat
     9961 gagtatttgt cgttccaaag atcagttcct gtttccaccc ccattttatt caaaatgatt
    10021 tgaccttttt cgcgagtatt ttttgaagtc acagaaatag tctcaccaga tttaatggtt
    10081 gcggtgattg gtgtagtgtc aatcgtataa ggagctggca ctgatttttc agtaatggtt
    10141 actttcgcgc catgaggaat ctcatccaat gtcgcaatgc cttctttatc ggtggtcacg
    10201 tcttttgcag gtaatgtttt tccaaagtct aaatggaaaa cggttcccgg tacaacattt
    10261 cctgtttctt tatcgacttt tttaatcttc aaagaaccct cagtttccac atcaattttg
    10321 attgtgtagg tattgggctt atcaattgcc cccgccatca aagtttgttg accagccatt
    10381 ttataagcta ccggtgttcc tgtattagct gacttcttta gggttaattc accactctct
    10441 tttgaattgg catttggagt aatcactagt tgattgccgt tcacacgata atcaatattt
    10501 gcgatattct cgacaacttc atcaaactcc gataaattca agccgttcgt atctgtcaca
    10561 gtagtcgatt gacctaacac ggttttcact gtggagttat ggaagcttgg tttcttttga
    10621 taatccgcaa tggcttgatt gatttttgac tcaatggctg cgatatttac tgcactacca
    10681 tcagaacgtt taattgagtg aagcgtgtag ccattaactt cttgccaaat catcttctgg
    10741 gccacaatat gtgtatccac atccgtacca gcttccttcc ataaaacgga aactaatttc
    10801 gctttctccg acatatcagg caatggattt ttctcataac caggagtgaa ctcattgtaa
    10861 atcggcaccc ctggctcaat acagaaaata tcttgcttca caccattgta aactgcatag
    10921 tgcggaacgg taacttctga catgggacga ccatcgctaa atgtaccttc gacagcatac
    10981 tggtgagcca aatccaattc cacagttacc gtttgtggat gagtaatttc atcagcaaag
    11041 gcaatcgttg ggccaagcga tccaccaatt gtttggcata atagactcag tgttgcgagc
    11101 aaacgccaat tcttaaatct tggctttttc ataaaataaa tctcctttcg tttttgggta
    11161 ataaaaaaga cgtccaaatt ttaattttga acgtctgatt tagagtgata ctatttagtt
    11221 ttctttgtct tggataggtt gtttctctgc ttataaattt tgattcttcg ataatcaaat
    11281 ccaatctctg ccatcaattc ctcccaattt ttgttcgttc ttttcataat tgtatcaact
    11341 gatggtagtt ttggggattg aactagtttt cgaaactttg tcattgataa atcatcctgt
    11401 agttttaatc ggattatttc attttttagt tctaacagca gttcgaaatc agtaaatcga
    11461 gatccatcat ttttttcttt aaaaagtgga cgtatatctc ctaacatctt tttaatggta
    11521 ctaagtgaag gaatattctt ccctactgat ttttgttcgt acattcgttg agaggttatc
    11581 ttctcgcact taataaaggc ttctactatt tccaataatt cttgttctga cattctagcc
    11641 cattttccat acccagtatt ttctcgtcct atactaatca ataaattctc ccatgatcca
    11701 tattttttgt taatgaacca catacttggt gcagaatttg gattttctga tagctttttc
    11761 tggtattgac ggctagatgt aattccgtgt ttttcgatga attcattaat tgttaaaatc
    11821 agttttaact cactttgatt tttccaaatg atacgtccat cattttgctt cacgataaaa
    11881 actcctaata gttatttaga tagaagccta tcacatcaac tttttagtag aaaagtacat
    11941 gttttgtctt ttttgctaat agctgaccct ttgagaaact tgggttcaaa agttgctaaa
    12001 ttcggtaaat gttttggtat ttttcgtatt atatttgtcg tcaagctgac tactaaattt
    12061 tgccctattt tgacgacact gatagtcaat gtcggacaat ctaggacaat ttttaaggga
    12121 atatgattga aaaatggtcc aaaacagcca aaaatggctt aaaactgtga tagagaaaaa
    12181 agaaaaagcc cttgatatac aaggactttt cacaaatttc agatttccaa ggaagttttc
    12241 cgcgtttatc ttctgatttc tttgatacga gcagcttttc cgtgtaatgc acgtaaacaa
    12301 aaccactata ttctattatg tatctaatac cctcaaaaca gctataaatc aacaataccg
    12361 acatataata aacactatgt tcaatctgag atatatcaaa ggtaaacgta tttttgcccc
    12421 ctttttgccc ctcaaatatc gctttacaac gagaacgtaa gttcgtataa tcgttttgag
    12481 gtgattttta tgatggaaga ctttattaga aaaaatatca gtgatgaata tgcagacttt
    12541 tatgagcaaa gcagcaaaaa agataaattc cagatggatg tttcaatctt agctatatta
    12601 gctttttccg aaaataacca acctgtaatt gcgaaaaaag aaacagtatt atctgaaggc
    12661 aaaataaaaa cacgatatat attagaggta gaaactaagt ttaaaaatag atcggagtaa
    12721 tggctatgct ttttaatgag acacaattat ggtttaaatt tgatccttcg aatagattta
    12781 tcaaagattt ttataaggtg tgggattcag aagttttctt tctagcaatc gaagatagct
    12841 tattaatcaa tctctactat tctaataaga actactttaa aatccctgct gcaaaaacta
    12901 gaatgaagaa agatgtatac tttttgtttg atatcgtgac tgacgtgccg gacgcacgta
    12961 gcgatcatcg gcgttatgac tatataaagt atactttcgt tgatccagaa aggta
//
LOCUS       DANKAY010000072        12969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000072 DANKAY010000000
VERSION     DANKAY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..779)
                     /locus_tag="PWY98_002031"
     CDS             complement(<1..779)
                     /locus_tag="PWY98_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2037770.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLS"
     gene            complement(829..2325)
                     /locus_tag="PWY98_002032"
     CDS             complement(829..2325)
                     /locus_tag="PWY98_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2037771.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2519..4141)
                     /locus_tag="PWY98_002033"
     CDS             complement(2519..4141)
                     /locus_tag="PWY98_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2037772.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4469..5782)
                     /locus_tag="PWY98_002034"
     CDS             complement(4469..5782)
                     /locus_tag="PWY98_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2037773.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(6027..6251)
                     /locus_tag="PWY98_002035"
     CDS             complement(6027..6251)
                     /locus_tag="PWY98_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037774.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(6271..6693)
                     /locus_tag="PWY98_002036"
     CDS             complement(6271..6693)
                     /locus_tag="PWY98_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2037775.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(6835..7545)
                     /locus_tag="PWY98_002037"
     CDS             complement(6835..7545)
                     /locus_tag="PWY98_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037776.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(7563..8060)
                     /locus_tag="PWY98_002038"
     CDS             complement(7563..8060)
                     /locus_tag="PWY98_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037777.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(8097..8549)
                     /locus_tag="PWY98_002039"
     CDS             complement(8097..8549)
                     /locus_tag="PWY98_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2037778.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(8684..9289)
                     /locus_tag="PWY98_002040"
     CDS             complement(8684..9289)
                     /locus_tag="PWY98_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2037779.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(9739..10359)
                     /locus_tag="PWY98_002041"
     CDS             complement(9739..10359)
                     /locus_tag="PWY98_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2037780.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(10361..11230)
                     /locus_tag="PWY98_002042"
     CDS             complement(10361..11230)
                     /locus_tag="PWY98_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2037781.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(11253..11660)
                     /locus_tag="PWY98_002043"
     CDS             complement(11253..11660)
                     /locus_tag="PWY98_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2037782.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(11679..12968)
                     /locus_tag="PWY98_002044"
     CDS             complement(11679..12968)
                     /locus_tag="PWY98_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2037783.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         3727 a         2758 c         2130 g         4354 t
ORIGIN      
        1 gataactttt ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt
       61 tctaaaagtt cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag
      121 tcctctcctg aaacaatata attatatgat ataagaacct cttttaatgt cttttggaca
      181 gtcggcatga tattggaaaa aattgtactg atttgttgag gaaaccttgc gtgttggatc
      241 accgtttctg ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt
      301 tttagtcctt ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc
      361 aaaatcgttt ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc
      421 ttttgcatac tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg
      481 aaatccatgc tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt
      541 tcagattcaa ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc
      601 ttctcgtaga tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct
      661 aaagcttcag ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga
      721 atttggagtt gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata
      781 caggaaaaac ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa
      841 agatctatat tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact
      901 atcttttttc aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa
      961 gccatatcgc ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca
     1021 taagtaacca atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat
     1081 ttttagatac tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc
     1141 tagtccaatg ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt
     1201 catgacataa cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc
     1261 cttcaaaaag ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc
     1321 ttttttatag acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg
     1381 caagcactgt tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct
     1441 gtaaatataa gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg
     1501 tttaaattgc agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa
     1561 tgcagaaaag ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg
     1621 agtagcacgg gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt
     1681 tagtgtagca atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc
     1741 ataaggcata cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc
     1801 ttggtagcgt tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt
     1861 ttgccacccc ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat
     1921 tactggctcg atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc
     1981 agcctcattt ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt
     2041 tcgaaaagct tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata
     2101 aaaatcaatc gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat
     2161 ctttttttct tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc
     2221 tgtcacatta ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac
     2281 tgctccgccc cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg
     2341 ctatcaacaa tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta
     2401 tttcggcaga agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata
     2461 acaaatacat aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt
     2521 attttacttc caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact
     2581 gtttcagagg ataatttgac acaataaccc ttaaagcttc acgtggaata gtgaacgtct
     2641 gcttttcact ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat
     2701 aggcaaagac atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt
     2761 catgttttct acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg
     2821 ctaacgcttc ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc
     2881 cagtagtaaa cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc
     2941 ttcctttcgc cttgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt
     3001 cgttatacat attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat
     3061 tcgtcatgcc taactcttct ccttgataga tataaggggt gcctttcatc atatggagca
     3121 agatagcaaa catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa
     3181 caattcgcgg aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag
     3241 atgtttgcca tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc
     3301 atttgtcttt gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata
     3361 attcatttct tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt
     3421 cgccaacagt catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact
     3481 catgcagttt aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga
     3541 tcacgtccat acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa
     3601 tctcttggcg aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt
     3661 gcagaaaata ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc
     3721 gcaatctatt aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag
     3781 gattttcttt atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca
     3841 ccaccagatc catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca
     3901 tttcttccat cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat
     3961 tgtcatcatt cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg
     4021 ccagataatc caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat
     4081 cttgaaaact tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca
     4141 tgattttcct cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct
     4201 ctcactaaaa attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata
     4261 aaacaaaaaa acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca
     4321 gggaagaatg ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt
     4381 attagctcaa aaaacggcaa aatataagaa actatttttt cgtttttttc tctaatactc
     4441 aggccagaac gctgatttca caacctcttt atttcacatt caatatccac atgaatccca
     4501 caaatacaaa gaacagacca tacatgattg gagatacttc ttttcctctt ttagctgcta
     4561 acatggtaat tggatagaag ataaaaccta atgcaatccc atctgaaata ctataagtca
     4621 aaggcatgcc gatcaagatc aagaatgaag ggatcgcaat ctctaagttt ccccattcga
     4681 tttgtcgcaa agattgtgcc atcaatactc ctaccataat caatgctggt gcagttactt
     4741 gtgaagtaac aactgatagt aatggagaaa agaacaagcc taacaagaag aaaatccctg
     4801 tagtaatagc tgtgatacct gaacgtcctc caacggcgat accagctgat gattctacaa
     4861 atgcaccaac aggagaagta ccaaataagg aacccgcaag catagctgtt gaatcagaag
     4921 ctaaagcttt ccctacacga ggcatcttgt tgtctttcat gaaacctgct tggtttgcta
     4981 atccaactaa agttcctgct gtatcaaaga aagtcacaag caagaatgtc agcactacga
     5041 cccacatttg aagagaattg atgtccccta catgttttaa tgcaactaaa aatgttggtt
     5101 tcaaacttgg tgctgcggca acaatttcag aaggcattgg aatcaaacct gtgaccagac
     5161 ctaaaatcgt tgtagctgcc atcccgatga agatgccacc tggtacacgc cgaacaagca
     5221 agatagcagt tacgactaat ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc
     5281 ctaatcctac taatgtagat tcattcgaaa cgatgatccc accttcgctc agaccaagga
     5341 aagcgataaa tagaccgata ccgccagaaa tcgcatattt caaatctgct gggatcgcat
     5401 caatgatcat ttcccgcagc ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga
     5461 atacgccggc taatgctgtt tcccatggaa tccccattcc taaacaaaca gaataagcaa
     5521 aaaaagcatt gatcccaaga gcaggtgccg ttccaattgg ataacgggct aataccccca
     5581 ttaaaacaca gccgatcgca ctggctaatg cagtcgcagt aaacactgct ccttcatcca
     5641 ttccagaagc acctaataca gttggattta caaataagat ataagccatt gagataaatg
     5701 ttgtaaatcc tgccagtacc tcacgtttta ccgtggtgtt caatttttct atttcgaagt
     5761 aggaaagtat tttttccttc atcttccatc cccctaaatc cgaacattat aaataaagtc
     5821 cgttaaaatt tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat
     5881 aagtaaacaa aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct
     5941 ttatctcaaa gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata
     6001 aagtttcttc attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc
     6061 tgttcttctt tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa
     6121 cctgtatcga ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt
     6181 gctcgaagct tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc
     6241 attagatcca ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac
     6301 aaaataattg ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat
     6361 ttctcctggt tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa
     6421 agaaggcggt ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac
     6481 gcctaattct aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc
     6541 gattgtttca gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac
     6601 gtcattgaca taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt
     6661 tgattttctt tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt
     6721 gtttgatact attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac
     6781 tttttttaca aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa
     6841 ttctgcttct gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg
     6901 atcgattttt tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt
     6961 gatggattca atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc
     7021 ttcacgccag ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc
     7081 cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc
     7141 ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc
     7201 aaaagacaag gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg
     7261 ttcttgattt gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt
     7321 gaatgtggta aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc
     7381 tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc
     7441 actttctttt gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc
     7501 aggaaatact tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt
     7561 gattagaata attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa
     7621 acatgttcgc tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg
     7681 tgttcccaaa caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct
     7741 gtatctagta aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc
     7801 tcgatttcta aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc
     7861 aggtaacccg caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt
     7921 agctcttctg taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc
     7981 ggtgaattat cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag
     8041 tctacatctg aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa
     8101 tagatccttt tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact
     8161 ttatttaaaa gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact
     8221 gcattggagt agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc
     8281 ggatagagtt tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg
     8341 gattctaatt tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct
     8401 agtttttctt ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct
     8461 cttgttaaat cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc
     8521 attccgatgt ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact
     8581 agttcacctc tttttgttgc aaatgcaact attttcaata aaaaaatcaa gttctttttt
     8641 aataactagt aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct
     8701 ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg
     8761 gcaactcttc tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg
     8821 cctcttcacc gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa
     8881 tatctttttg tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc
     8941 ctgtttcttc atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa
     9001 atccaccagg aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat
     9061 cttttttgat ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg
     9121 tgagcgcttt tatttcttcg aaagtttctt ttcctatttc actgatcagt tccaatgaaa
     9181 tcgtccggat ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag
     9241 cttgagaaag tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt
     9301 cttttttagc aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa
     9361 ataaatataa tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac
     9421 attcgacttc tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa
     9481 gccccaccaa acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg
     9541 caggacagct ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca
     9601 caagtcacaa tattcatcaa acatgagaag ctgttttttt gaaattgctc cttgtgactc
     9661 ggagctgaac gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga
     9721 tattaagcca tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag
     9781 tggagtgtaa tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg
     9841 gaatttgtca tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt
     9901 ggtccctttc gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct
     9961 caaatgacga tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc
    10021 tgctgttagg cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg
    10081 tgctttttgc ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat
    10141 atacaaatca agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg
    10201 ttcttccaaa ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt
    10261 gatggcatac atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt
    10321 accaggacta ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa
    10381 ttggactttc caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt
    10441 catttgctgg aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt
    10501 ctttagctac tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc
    10561 cgttattgat caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta
    10621 gggggacatc aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg
    10681 atttgaattg tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt
    10741 ctacaaattt cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga
    10801 ttccttcttc tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat
    10861 gggcatattc tactactgct tttgtcttca ggatattttc tttgaaaggc agatgggacc
    10921 catcatacat gacagaagta tatccgctgt cgattgcttc tttgatatca tcaaagtctc
    10981 gggcgtggtc taagtgcaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta
    11041 cattgactaa cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga
    11101 tcggcgaacc catttctttt gctgcgccta gcatagcagg aagcatttct aggttatgcg
    11161 tattaaaagc tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg
    11221 ttgtatacat cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt
    11281 tttgcaattt gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca
    11341 ccagcacgag cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca
    11401 tctccgatcg tctgtttatt ttccaaactc ttttctaaat attggatggc ttgatcgttc
    11461 acatacaatt cagaaaattt aagtccaact tgttcataaa gtttcgctaa tttatgctta
    11521 tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc
    11581 gcttctggcg atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt
    11641 ttcttaaaaa atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg
    11701 cttaagatcc aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca
    11761 ttgttgaatc ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag
    11821 aaaccatgag caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat
    11881 atccctgctg tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag
    11941 ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct
    12001 aaaatgacag catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca
    12061 tcaggaacta atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga
    12121 cgtacaccag ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca
    12181 aacatgatat agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa
    12241 acagtgatga tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga
    12301 aattcgaaat gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca
    12361 ccaaaaagct tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa
    12421 gcaatatcat ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca
    12481 atggcgaaac aagctccgat aaaaccaccg accaagatca atacgatccc tcgcagaccg
    12541 gcaaaatagc caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa
    12601 aaaatgtact tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag
    12661 aacgtcagtg caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat
    12721 tcaatagaag ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa
    12781 gttaaacttc caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc
    12841 tttaacgtgc cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata
    12901 gcaatcaatg caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata
    12961 acgcccatg
//
LOCUS       DANKAY010000073        12858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000073 DANKAY010000000
VERSION     DANKAY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..1108)
                     /locus_tag="PWY98_002045"
     CDS             complement(<1..1108)
                     /locus_tag="PWY98_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2037784.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDY"
     gene            complement(1230..1826)
                     /locus_tag="PWY98_002046"
     CDS             complement(1230..1826)
                     /locus_tag="PWY98_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2037785.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2026..4668)
                     /gene="alaS"
                     /locus_tag="PWY98_002047"
     CDS             complement(2026..4668)
                     /gene="alaS"
                     /locus_tag="PWY98_002047"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2037786.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5056..5487
                     /locus_tag="PWY98_002048"
     CDS             5056..5487
                     /locus_tag="PWY98_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037787.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5532..6881)
                     /locus_tag="PWY98_002049"
     CDS             complement(5532..6881)
                     /locus_tag="PWY98_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2037788.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6951..8114)
                     /locus_tag="PWY98_002050"
     CDS             complement(6951..8114)
                     /locus_tag="PWY98_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2037789.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8243..8566
                     /locus_tag="PWY98_002051"
     CDS             8243..8566
                     /locus_tag="PWY98_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037790.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8749..10098)
                     /locus_tag="PWY98_002052"
     CDS             complement(8749..10098)
                     /locus_tag="PWY98_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2037791.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10167..11120)
                     /locus_tag="PWY98_002053"
     CDS             complement(10167..11120)
                     /locus_tag="PWY98_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2037792.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11222..12412)
                     /locus_tag="PWY98_002054"
     CDS             complement(11222..12412)
                     /locus_tag="PWY98_002054"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2037793.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3691 a         2711 c         2223 g         4233 t
ORIGIN      
        1 ggtaatctcc gattttttct tctatttgac tacgtaagat tctttctagt tctgtatcaa
       61 caaactggaa tttctcaggg accactacat caaattgcag cgtttttcct tgatgaatta
      121 ctctaaaatc atgaatatta agccctgtac ctgtttcaag aagaatttgc tttaactgcc
      181 gcaagatatt atagtatttt tcgttatcaa caggcattgg atccaagtga cagacaagtt
      241 ccacatccaa agacttttta aagtcttttt ctatcagatc aataatctta tgagccacat
      301 tcaaattcat tgattcatct acttcgatgt ggactgatgc aaagcgttta ttgggtccat
      361 agttatgcac caataaatca tgatagccaa gtatatcatc gcaagcattt aaatgctctt
      421 ccatttcttc aatctctgct ggcgttgggc gacttcctaa tagttcgtat acaaaatttc
      481 gaagcatcgt caatccacta taaaggatat ataatgctaa agcaaaacct atatatccat
      541 caatacgcca tccagttacc cattcgattg ctgctgaaag caatacagcc agtgtagtaa
      601 agacatcatt ataactatct gttgctgtag ctctcaatgt ttcagaatca atggattgcg
      661 caataacctg atacattttg ccttgtgcaa atttcaaaaa gatggaaatg attaggacaa
      721 aaaagacgat gggtgttaaa gagacatttt ctggatggat gattttttca gcagaagact
      781 ctaaaaattg aaatcctaca taagtaatga tgatcgaaac aaataaccca ctgatatatt
      841 cgaagcgttc gtgtccataa ggatgttcct gatctgctgg ctttgcagca attttaaatc
      901 cgatcaaagt caatatggat gaagcagtat ctgatagact gttcactgca tcagccataa
      961 tggagacact tcctgaaaaa agcccgatca ttagcttaga gacaaacaag atcagattcg
     1021 taaccaatcc taagataccc gctagtactc ctttttttgt ccgctgttgc ggtatttgtt
     1081 tctttttttc ttcttgtttc ttagccaagt tttttacccc ttctagtttg cttgttgctc
     1141 aggtaaaaag atggagagga cgattgtttt cctctcctct cttccttttt ctatctatcg
     1201 tgcgtcctat tactattaaa agatggtcat tattctggtt tcaacggtgt tgctgcaaat
     1261 aacgcttttg ctagtaatgg tgttatgatt cccgcaccta ttgcttctgg tatcccattg
     1321 attgctgcaa cccctgctaa ggttttaaat aaaagagcag agtctacacc ataggcatct
     1381 gccgttgcac ctgtataaaa taatcccatg aatcctaaca ctaagactgt attgaccaat
     1441 gaacccagta cccctgcaaa ggctgagctg atcgttattg atttattttt ttgataaatc
     1501 cagtggaaaa ccaagcctgc tactaagcca acaagtactc tgggaactac cgagataatc
     1561 ggattcgtga acactaatgt atcaaatggc gttgttggca tggcataggc acgaacaatt
     1621 gtagcgattc cccaaaacaa gccaatcacc atgccatctt ttgggcctaa tactactgca
     1681 gcaataatta ctgtgatatg gataattgtt aaactaatga atcctaatgg aataaacccc
     1741 agccaaggaa ccatcgcttg tacgatgatg attgctaaca agatcgcacg taagactagt
     1801 cgaaaggtct tatttctact attcattttt tcaccccttt ttatttatac taatcgtaaa
     1861 aatttctgtc actttcgtca actaaagacc atccgaatcc gaccatttga ttagatgcgc
     1921 catccaattg aaaaaagtat gagggcaaga cttgtttgtc ttcgccccca taatcatgtt
     1981 tcgttcaatg tttgaacttt tgtttttctg tcaaacgtaa acgccctatt gttgcgcaac
     2041 ccattctttc gcagctttaa gtgcatctgg aataccagca ggatttttcc cgccagcttg
     2101 tgccatgtct ggacgtccac caccgccacc gcctactttc ggtgcgattg ctttaatcaa
     2161 gtcgccagct tttagtccag cttcatttga ttctttcgtc attgccgcta gcagactgac
     2221 ttttccttct tgttcgtttg ctaaaacaag aatatctgaa atattttttt gtttccattg
     2281 gtcagctaat tgacgcagct gattcatatc tttcactttt acttgtgctg caatgattgt
     2341 ttttccattg atcgtttctg gatctttgaa tacatcacca gcttgttgat tcgctaactt
     2401 gctagctaat tgttcatttt ccttttgtag ctctcttact tgctgttgca attgttccgc
     2461 ttttgaaaca acttctttca attgaggtga tttaacaatt gttgctactt gttttagttg
     2521 tttttcttct tcttcaagta gttcgtacgc ttctttactt gttacagcct cgattcgtcg
     2581 cacgcctgca ccaattcctg attcagaaac gattttgaag attccgatgt tttctgtatt
     2641 tgctacatgt gtgcctccgc acagttcgat tgaccagccg ccaatattta ctactcgaac
     2701 gtcatgtccg tatttttcac caaacaaagc cattgcaccc atgtttttgg ctgtatcgat
     2761 atctgtttca attgtttcaa ctgggatagc ttcccagatt ttttcgttga cgattctttc
     2821 catttcagct aattcatctg aagtgatttg accaaagtgg gtaaagtcga atcgtaaatg
     2881 tccaggagcg actaaagaac cagcttgatt tgcatgctcg ccaagtactt ctttcaatgc
     2941 cttgtgcagt agatgggttg ctgtatggtt tttgatgatc cgattgcgca tcgattgatc
     3001 gatcaataga tgataggttt gtccttcact cattggtgct tttacttcta cggtgtgcat
     3061 aaattgacca ttaggcgctt tttgaacatc tgtaacaact gctacgattt cgcctacttc
     3121 attcatgatc catccttgat ccgctacttg cccacccatt tctgcataga aaggtgtttg
     3181 atcaaagatc atttgagctt caccttctgc gacttcattc aaaagagact cacctttaac
     3241 gatgaccaat aattgactat ctgattcttg ttctgtgtat ccaatgaatt tactttctac
     3301 tttgatatct gtcaataaag cagattgtac ccccattgat ttttcagtac tgcgggcaga
     3361 gcgagcacgt tcgcgttgtg catccatttc tttttcaaaa ccagcatgat caactttcag
     3421 tccttcatct tctgccacct cttccgttaa ttctaccggg aagccatatg tgtcgtatag
     3481 tttgaagatg tcttttcctg ctagagtcgt cccgttttcg ttttttactt cttggatcac
     3541 ttgatttaag atttccaaac cttcattgat tgtttcattg aaacgttctt cttctgtacg
     3601 gataactttt tcaataaatt ctttttgttg aagtacttca ggataataac ttaccatgat
     3661 ttccccaaca acagggacta aacgatataa gaatgcttgt tcaataccta attttttccc
     3721 atgcatgaca gcacgacgaa gcaaacggcg taagacatac cctctccctt cattcgatgg
     3781 caatgcaccg tcgccaatag caaatgaaag ggcgcgaata tgatctgcga ttactttgta
     3841 agaaatatct gttactgggt tttcaccata ttttacttgt ttgcttagct tttctacggc
     3901 gtggataata ggcataaaca agtctgtctc aaagttcgtt gacgcatctt gaacgattga
     3961 gaccatgcgt tccaatccca tccccgtatc aatgttttta tgaggaagtg gttcaaatgt
     4021 gttatcaggc ttatggttga attctgaaaa tactaagttc cagatttcta aataacgttc
     4081 attttctcct cctggatagt tttcaggatc gtcttctgct aaatcgttat aagcttctcc
     4141 acggtcatag aaaatttccg tatccggacc acatgggcct gcaccgatat cccagaagtt
     4201 gtcttctaca tcaataatgt gatctagtgg caggccaact tcttcatgcc agatgcgttt
     4261 tgcttctgta tcttttggat aaactgttac ataaagtttt tctggatcga atgccatcca
     4321 ttttggatca gtcaaaaatt cccatgccca gtggatggct tcatttttga aatagtcacc
     4381 aatcgaaaaa ttacccagca tctcaaacat cgtatggtgc cgtgctgttt taccgacatt
     4441 ttcgatatca tttgttcgaa tagatttttg ggcatttgtg attcttggat tttctggcac
     4501 aactgaacca tcaaaatatt ttttcaatgt tgcaacacca gagttgatcc acaataaggt
     4561 tggatcgttc acggggatca aagacgcact aggttcaatc gtatgtcctt tagattggaa
     4621 gaaatcaaga aacatttgtc gaacttgtgc actacttaat tgtttcatgt cattacctgc
     4681 tttctataaa ttaaatacaa aaaaacacca tcacagccaa ggacgaaaac tcgcggtacc
     4741 accttgattg caatgatgtg taatcattac ctcttaaact cgataacgct gagctgcgtc
     4801 ggtatttcta ccaaaataga aaaagggagc aatttgatta gtgactattc ttttttcagc
     4861 caagaaagaa ttttctgtga gtcttaaaaa acaaatcata tccttttcgt acttaattag
     4921 tatacgcatt ctattgatca ctgtcaatat ctcaattttt attttcattt ccttttggtc
     4981 atactccacg atttttggta cgataagaat aaaggaatca atacatctct gctattcatg
     5041 acaaggagga atatgatgaa aaaaatagtt ggagcggatg aatggatacg cgcagctagc
     5101 tgctttttga gatatcaagt ttttgtatta gaacaaggga ttcctgcaga actagaattc
     5161 gacggaaaag atatcccgca acaaacctat tatctatttc ttgatcaaaa aagaccagta
     5221 gctactttgc gttatgaaaa gaaaacacct acttgcttca agccggaacg attttgcgta
     5281 gcaaaagaat accgaggaaa agggttaggc ggtcagttgc ttgaagctgc tgaaaaacgg
     5341 gcggtttcag aaggttgtca gactagttat gtagtagctg aaatgacggc tgtagatttt
     5401 tatgaaaaac aagggtatca tacttgcaca gaacctttta ttgaagatga agtactttgt
     5461 atcggaatga aaaaaagtct agcttaaaaa caagagattg tgacacgcct tttgtcacaa
     5521 cctcttgttt tttatttatg tgaatttttc ttatttttac gagcagtacg gctttgctgt
     5581 ctgcgttcga ttttgcgttc ttttttattt ttttctgcaa ttgcccaatc gatttttttc
     5641 ttataaccag gcttgatctt tttctttttc ttcttcacaa gaccaatcag cgtgggatct
     5701 aatacatctt ttgatttttc tcgtttggca cgtctattac gatcgtatgt tttgacgatt
     5761 tccccgtttt tgatttcttt tggttcaaat gagacgccca atttttctat cgcatcaatg
     5821 gcttgctcat catttggcga atataacgta atggccgttc cttttaagcc attacgtcca
     5881 gtacgtccga cacgatggat aaagaagtct agatcttctg gaatttctgc attaataaca
     5941 tgagagacac cttcgatatc gatccctcta gcagctaaat cagtcgctac aacatattga
     6001 tactccagat tctgtacttg tcgcatcact cgttttctct ctcttggggt gatgtctcca
     6061 tgaatctttg ctactttcaa gccttggttt ttcaagtaat cggcaatttc atccacatgc
     6121 tgttttgtat tggcaaatac gattgctaaa taaggatgac caattgtcag cagttgataa
     6181 atcatttgat tctcatcttt accttttgtt gaaatcagcc aattttcaat gtcttcagaa
     6241 ataactgctt ttggtttgat ttcttcaatg attggatttt ccatgtattt tcgtaagaat
     6301 ggacgaagtt tttctggaat agtggcagaa aaaactaaaa actgcagctt ttctggtaat
     6361 cgacttgcaa tttggtctac ttcatttaaa aaacccatat ccaatgtcat atccgcttca
     6421 tcgacaacaa atgcaaatgc ggtgtgaatc ttcagcgctt gttcattcat catgtctaat
     6481 attctaccag gcgtgcctac tacaacctgt ggctgttggt gatgcagacg atttaattga
     6541 cgctgtttgt ctgtaccgcc gacaaaattc gagacacgga tctccggtgt agaaaattta
     6601 gcgatttgtg tagctgcttc atagatctgt gcagctagtt ccctacttgg agctgtaata
     6661 acaacttgta cttcctcttt ttctgcattg attttttcaa acaaaggcaa caaaaacgta
     6721 tgtgtttttc ctgtaccagt ttgagattgc ccaatgatat tttttccttt tgcaattaaa
     6781 ggaatcaaac gttcctggac ctctgtgggc tgtttgaagc cctttgcggt taacgcttca
     6841 ttgataaacg gtttgaagcg gaatatatca aaattattca ttcgtgcacc tcttctcttt
     6901 aaattatcac gactttggca ttataacaca gacaaagcgt ttttgctcag ctaatttcga
     6961 gaatcatcat cctttgtttg gccaatcgtg ctaacagtat atgtgtgact agctttttca
     7021 taaaacagaa tccgtttttt tctcaaaaat gatagcaaga tatttaaaat aaaaatacct
     7081 tgtagtaaca agacaccgag agaaatgaat actgcactca tctgcaacac cgtattcgtt
     7141 gtcgaattca tgatttgctg aagcaactgc cccaaccagc ttaaaccata aaaaagaaag
     7201 tacagatacc cgtagcggat catcaatgct tttaaagaaa ttcgttcgcg gtcttcttcc
     7261 accaaacgga tacgcacaat ttttttgcca agtgtccatc cattggttat aaacggtaca
     7321 atcataaagt atacccctac ttctaataaa agccaccaga tatgagaagt aactgcccct
     7381 gaatcgaacg caattttaaa gaaaatcgcc gctaccaaat taataagaga taaaaatgtc
     7441 cagtcgatca aataagcgac caagcgtcga gtcaatgaga cagtctttcc tttttcataa
     7501 gaaagttcat ccattcgtct tcttgtagga agcataaatg taaataacgg cgtaacgaca
     7561 aaaccaatga ttccacccaa tgtatttgtc actaaatcat tcacatcaaa taagcggtat
     7621 ggtcttgggt agataaaata aagtccactt agctgcgtca attcaaaaaa tagagaaagg
     7681 cagaaactag caatcatcgt tttcataaaa gaaagccgga aataatatct taaataaatg
     7741 ccaaacggta tgagtaataa aacattaaaa gctggttcca aaaatacact ctgcttcatt
     7801 gccggcaggt aggtactagg ttcagacaag accaataccg tgttgtctct gaaatgttgc
     7861 catgaagcaa ataaatgcag ctccatcttt ggccctgtca tttgggcaac cgtctccttt
     7921 gccggaagtg gtaagatcac caaaaaatac gcacacaata aataaaaaac gaaagaatac
     7981 aaaataactg cccttgttac tagaaatgtt ccatactttc gatattcata aataaaaaaa
     8041 acagatgaga tcgctaaggc taaaaatgga aaaacaatca gcgcaaaacg aatcggttcg
     8101 atataacttg ccatatgact cctccttgat tctttgtatc tctcttatct tagcagtcct
     8161 ttgctgtttc aagcaagtaa cgaggctatt atttctcatt tctttcaaag taaagtaaga
     8221 taaacacaaa gggggtgtcg agatgctgaa tatgtacttt gtttttggcg tacctatttt
     8281 tttactgttc ctttatgcta cgatcgctta tgtaagaaaa agaactacaa ttcattattt
     8341 aggatttatt ctcctgatta tttcgggttt catgctagtc ttcaatcttc agacttggca
     8401 gcaagcactt cttgaaatgg ataaaatgac tccacatgct ttgtctaaag tgcttggcta
     8461 ccctgtctat ctgatttggc ttcccatttt catttctggt tgcttggttt tattaaatat
     8521 ttatcgcggc gtacgccgaa tagttcagtt acgcaaatca aagtaaaaga cagaaatcgg
     8581 ttgagcccga tttctgtctt ttactttgct atttcatcgt agacaaaacg acatttacgt
     8641 gatcaaaatt aacgagtact tttgacattt cttccacgta ctcctcattt aaataaaata
     8701 ttttgtttgg cagagaagtg cagttctaat aaaattcgaa tgttttagct atctacgttt
     8761 taaatagaga taaatcggga taccagcaag tgtcaatact atccccatcg aagctaaaaa
     8821 ggtctgagtg ataagcgtgc ttgatacgat aaaagtccct ccaatgattg ctactagtgg
     8881 aataaacgga tacattggaa ctttgtatgg tcgaaacaaa tcaggttctt ttttacgaag
     8941 aagaatgaca ccaacaaaga ccatcgtata aaaaatccaa atgacaaaga tcagcatatc
     9001 cgttagtgta tcaaaaccac cgatcatcat catgccaata gcgatgacta gctctaagat
     9061 acctgcggct acaggaactt tagttttatc atgaagttta gcaaagagct tgctaaaagg
     9121 caggttgttt tcttttgcca taacataagg gagacgcatt ccagtaaggg tatagccatt
     9181 gatggttcca taaacagaaa tcaagatacc aactgtgacc agtcttccac cgaagccgcc
     9241 aaaaatcatt tttgcaacgt cacttgccgc attgctgttt cctgcgactc cgtcgataga
     9301 tgcggttctt aagaacaccg catttactaa taaataaacg atcatgattc cgatgattcc
     9361 taaagaaatt gcttttggca aatctttcgc cggctttttc aattctcccg aaatatttcc
     9421 tacgtgtatc caaccgtcgt atgcgaacat cgttgctagc aagcccgctc ccaaggctga
     9481 gaaaaatgaa agattttctc ctgcttggat cggaaacagt tggaagtcta ctccttcttg
     9541 tcggaacaaa ccgaaaatga cgatcacaaa caaaggaata agcttgcaga caagcgtaat
     9601 cgactgaaaa gcccctccag ctttagaacc taaaaaattg atcaacagga tcgatacagc
     9661 agctgtaaca gctacgggaa caatcattga ctgggaaaga ccaaatagat tgacgaactg
     9721 tgtaccgaaa atgatcgaca aggctgctac atttgctgga aaataaatca cgacttgcgc
     9781 ccagcccaat aaaaaagctg ctgtatttcc gtaaatccgt tcgatatatt taatcatccc
     9841 tcctgtttct ggaatagcag ctgccagttc agctcctgtt agtcctgcac agacagaaat
     9901 cataccaccg agaaaccaag aaaacatatg caaactggcg gatcctgtaa cttctgctac
     9961 gcttgcggct ttaaaaaata cccctgcacc gatcactgtg cccataacag tcgataaagc
    10021 agtaaaaaat cccatcgtac gttttaattc attttctatt tttacttctt ttactggcgt
    10081 ggcttgatat tcttccatcc cttgtcccct ttcataccat agccaatccc aaaagtataa
    10141 aaaacttttt ggatgtggtt cttgttttat accgtctcta ccaatttttc tttcagtttt
    10201 tccaacatat cctttgtcat cacatctaag tcgtatttcg gcgaaaagcc ccattcctct
    10261 tgcgcacaag aaacatctag tgaatttggc caagattctg ctattgactg acgtacagga
    10321 tccacatcat aatccatttc aaaatctggt atgatttttt gaatagatgc tttgatctct
    10381 tccgggtcaa aagacatcgc cgatatattg aaggcatttc gatgaaccaa tcgatcagga
    10441 tctgcattca tgagttttat aatggcatcg atcgcatctg gcatatacat catatccatt
    10501 gatgtacctt gatcaatgaa actagtgtac tttccttctt tgattgctga ataataaata
    10561 tcgactgcgt agtctgtcgt accgcctcct ggcaaggttg cataagaaat caatccaggg
    10621 aaacggacac cacgagtatc tacgccatat ttcacataat aataatcaca tagtagttct
    10681 ccagctacct tcgttacccc atacatggtt gtaggacgct gaagagtatc ttgtggggta
    10741 tgatcttttg gagtatttgg accaaacgcg ccgattgaac ttggcgtaaa aaatttcagc
    10801 tggaattctc tagcgacttc caatgcattg accagcccag tcacgttcaa attccaggct
    10861 aattgtggtt ttgcttctgc aacagctgac aaaagagcag ccaaatgaat caatgtgtcc
    10921 acttgatatt gttctgctat gctgcgcatt ttttcgtaat ccattacatc taggatctca
    10981 aagatatcgt ttttctcaat gacttcaggc atgcgtatat ctgtcgcaat gacattttca
    11041 ctgcctagtt cttgacgtag ccgtgtgatc aactctgaac caatttgtcc taaacatcct
    11101 gtcaccaata cttttttcat gatgatcctc cctgatcttt tttactacta tcttcagatg
    11161 attaacaata tatgcgtgaa catgttctct aattttaaat agacgaaaaa actaaattta
    11221 gttaaataat ttccatttct tttcctattt tttgataaac tgctactgct tcatcaagca
    11281 tttccctcgt atgttccgca gtcggcatat ttctgatcct gcctgtccca agaggcacag
    11341 ttggataaac gattggttta gcataaatcc catgttcgat cagcttctta gagaaagtct
    11401 gagtcacttt ttcgtctccc aaaatgactg gagtaatagg tgtttcagac ataccgatgt
    11461 tatagcctac ttttttcaat tcttctttaa aataattagc attttcccaa actttttcga
    11521 caagttccgg atgttcttgc atcaaagtaa tcgaagccaa tgcagctgca gctgcacctg
    11581 gagttagcga agtagaaaat aggaatggcc gacttctagc tttcaaccag tcgattagtg
    11641 ttttcgatcc agctacataa cccccaacaa cacctatagc tttcgataac gtgcccattt
    11701 gcatatcgat tttgtcgctt aatccaaaat gcttgacggt tcctgcacct tttccagtta
    11761 cgcctgagcc gtgcgcatca tcgacgtagg tgatcagtcc atatttttca acaatcggaa
    11821 taatttccgg caatcgtgca atgtctccat ccatggagaa aacaccatct gtgatataca
    11881 tgattttttt atatttctta ctttctatcg cttcctttgc ttttttctca aggtctttca
    11941 tatcttgatg tttaattcgg atgatttttg ctccggataa gcggcaaccg tcaataatag
    12001 atgcatggtt taattcatcc gataagatag catcttcttt tgtcattact gcagagatag
    12061 cgcccatatt gcagttgaat ccagattgaa aggcgattgc tgcttctgtc cctttgaatt
    12121 ctgcgatttt ttcttctaat tgctgatgga ttttcagcga accattgatc gttcggactg
    12181 ctcctgctcc tacaccataa gtttcggttg cttcaataca tgcttttttc aattcttcgc
    12241 gattggcaaa tccaagataa ttgttggatg ctagattgat catctttttt ccgtctacga
    12301 tgatacaagc accattttca ctttctagta tgtcgatcgt attgtataac cccttttctt
    12361 tcagttgatc caattctttc tctaaataca ttgttagttc tttggaagac attgtctcca
    12421 cctccatatt catctgtctt gcagaacata ccaaacatat atcgtgtcat tcatctacaa
    12481 agcaagtatg ctatattttt gataacgctt acactgatta tgagaattat ttaaccacat
    12541 tttttattta cagtatcttt ttactttcaa atgacttata tagtatgaac aattaccttt
    12601 tagataatga tctattttac tctccctggg cagataaata caacacaacc attgatagaa
    12661 gccctttcgt taaaatcaat tgtattctga atatttaatt tatttattgc atagaccaat
    12721 aaaaatacat aaaatttcca tcttcgttta tcagcaattt ttaattttac tctctgaata
    12781 tttcttaaaa gttttacttg ttgaattcga aaaaaggact gtgacaaaag tcttgtcaca
    12841 gtcctattct tccgaata
//
LOCUS       DANKAY010000074        12834 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000074 DANKAY010000000
VERSION     DANKAY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(459..884)
                     /locus_tag="PWY98_002055"
     CDS             complement(459..884)
                     /locus_tag="PWY98_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037794.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1258..2229
                     /locus_tag="PWY98_002056"
     CDS             1258..2229
                     /locus_tag="PWY98_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2037795.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2261..3658
                     /locus_tag="PWY98_002057"
     CDS             2261..3658
                     /locus_tag="PWY98_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2037796.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3817..4440
                     /locus_tag="PWY98_002058"
     CDS             3817..4440
                     /locus_tag="PWY98_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037797.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5189..7282
                     /locus_tag="PWY98_002059"
     CDS             5189..7282
                     /locus_tag="PWY98_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2037798.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            7442..8308
                     /locus_tag="PWY98_002060"
     CDS             7442..8308
                     /locus_tag="PWY98_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2037799.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(8330..8767)
                     /gene="ndk"
                     /locus_tag="PWY98_002061"
     CDS             complement(8330..8767)
                     /gene="ndk"
                     /locus_tag="PWY98_002061"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2037800.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            9426..12104
                     /gene="mgtA"
                     /locus_tag="PWY98_002062"
     CDS             9426..12104
                     /gene="mgtA"
                     /locus_tag="PWY98_002062"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2037801.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12191..>12834
                     /locus_tag="PWY98_002063"
     CDS             12191..>12834
                     /locus_tag="PWY98_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2037802.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVI"
BASE COUNT         4284 a         2155 c         2678 g         3717 t
ORIGIN      
        1 aattactacg tgatgtagaa taagcctttc tatcgtttgg ttattttata ttcaaatatt
       61 tgaaataaat aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta
      121 ctgatgaagc ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt
      181 gagttttgct tggatttgtt tatcttcttc tactttttac ataattttga acaggataag
      241 attcaaatgt tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc
      301 ttccttggtc attaaattct gtatggatat ttttgttgtt attacatgta caataatcag
      361 gactatcgat catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa
      421 aagaagggga gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca
      481 tcctcatcct tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta
      541 ttttcttttc ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc
      601 actgtttttt cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc
      661 cattccattt ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt
      721 acttttagcg tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa
      781 tcggttgttt cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc
      841 aaaagtcccg cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt
      901 gataggataa agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa
      961 cgcacaaaac acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat
     1021 ccgccacttt cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt
     1081 aaaaatgaac cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct
     1141 actgaagact ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc
     1201 atcataatcg aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg
     1261 gcatcgatcc gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac
     1321 ttgaatggtc agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg
     1381 ttggactata aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt
     1441 ggacttttga taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa
     1501 agagtagctg aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa
     1561 aaaattgaac aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc
     1621 ttatccgaag aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc
     1681 gcattgaatt gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa
     1741 agtgtagcaa aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt
     1801 gctgccactc aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc
     1861 caaaatgatc catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc
     1921 tattacgggg caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac
     1981 aatatgtcga ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat
     2041 attgcgttca gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc
     2101 acgatcacgc caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga
     2161 atcaaaaaag aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga
     2221 atcaactaaa acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc
     2281 agcaaatttt tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg
     2341 cgatggaaaa ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt
     2401 tgatcaagta ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca
     2461 gttgatgaaa cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat
     2521 acctgatgaa tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga
     2581 tgagttgatt gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat
     2641 gtatctgcaa gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata
     2701 tgccaaaagt tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga
     2761 accaatcgtt ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga
     2821 tatgaaaaaa gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa
     2881 atcttaccat gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag
     2941 ctatccaaga gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt
     3001 cttcaatcgt tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga
     3061 gatagtaaaa gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa
     3121 agaaaataca gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa
     3181 agaaacacca atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga
     3241 aatgcctggt cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta
     3301 tgatattttg atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa
     3361 cggtatgggt gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga
     3421 ttatcggatc gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg
     3481 tgccaattgt ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc
     3541 atataagaat cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat
     3601 caaaaaatcg ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga
     3661 aagaatagtt gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag
     3721 ttcaattatt tacgatcttt ttcaactttt ttgtctttct atttcatttt ttgtatataa
     3781 tagaatagtc aaaattaaat aggagtggag gaaagcatga ggaaaaaaag accgtttctt
     3841 ctcttttggt tattcggttt tttcttactg cttattttat ccgcagcatt ttcaggaaaa
     3901 atgacagcag atcttgctca gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta
     3961 gataatttca aaaaagaatt ctcaacttat tggtgggcgg agtataaaat aactgccaat
     4021 tacgaaaaag gcgaattgga catttatttg ccaaaaggaa aagaagaaaa tcaatttatt
     4081 aatatcgatg acgtacttag tgcgatttct ctcagacaat atcaatttaa agacaagcat
     4141 ttgaccgttt tgcttttgac tcatgatggc gacaaagtag cccgtttgaa aaatggaaaa
     4201 gtgatcaccg cttattctga aaaagtagac aaaaaagtgg tcgccgatcg tttagcggaa
     4261 tggaaaaaga caaatgaaca agtcaagaaa acaactgtct cttctcaaaa aaacgagtcg
     4321 acaggaacaa gtccttcaga ttctggcagt ggacaaacga taacgggaag tgaagaaaat
     4381 caagcctcaa ttattacgaa tgagtcatca caaggacaat atcaatccta tggacaatga
     4441 aaaacagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggcaaaat
     4501 agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
     4561 gtatcaagag gtcttgagcc tgccgtttaa ctctgagcca caaggagcaa tttcaaaaaa
     4621 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
     4681 cgtttaccaa gaggttgtaa aattagcgtc ctgacataat ctaataccga gggtcagctg
     4741 attgaacacc gtgtatcaag aggtcttgag cctgccgttt aactctgagc cacaaggaac
     4801 aatttcaaaa aacagcttct catgttttat aaatattgtg acttgtggcc gaggagttgg
     4861 caggagaaca ccgtttaagg aagaagttgt gaaaaagctg tcctgcatca agaaataagg
     4921 agctgcgaca agaattttgt cacagcctct ttttttgtac gaataccgag aaactggctt
     4981 ttgaataccg tggagtgaag cggtcaccac aagactttta tctcttcatt agtttaatcg
     5041 cttacatcgc agcggtttta tgctgtgaaa agtggacgga ataatcttta gaaagaataa
     5101 aaaaatctta agataacaac ttttttagga aattttcatt tttcaggagc aaaataagaa
     5161 aaaatgtgaa tagagagaag tgatatacgt gacacaactt aaaaaattgt gccaaaatcg
     5221 tttagcaatc gttttgacag ctgttttctt tttctggctg aagacgatca ttgcctacta
     5281 tgcagatttt tcattaggtg tggaaggtac gatccagtac tttatcttgt ggatcaaccc
     5341 aattgcgact accttattgt tttttggcct ttctttgtat attaaaaagc caaagccagc
     5401 gttagctatt cttttgatca ttgatatttt aaatacgctt ttgttgtact tgaatattat
     5461 tttttatcgg gaattcacag atttcattac agtcaaatct gtattaggct tttcgaaagt
     5521 gtcgcaagga ttgtcaggaa gctccttttc tttgatgaag ccccatgatg tcatctactg
     5581 gctggatatc gctgtgttca ttgggctact ggtttggttg aaggtcaaga aaatacctat
     5641 caaaagcaac cctgtcggca aaccgatggc tatcgctgtt acctgtcttt cagggcttat
     5701 tttttcagga aatctagcgc tgagcgaagc caaccgtcca caattgctgc agcgaacatt
     5761 tgaccgttca tatattgtca aatatctagg aatcgatgcg tatactattt atgacggaat
     5821 caaaacagga atgactagca gtgttcgcgc gcatgcaagc agtaatggga ttgatgaagt
     5881 tttagattat acaaagaaac attatgcaga gccaaatccc gaaacatttg ggatcgctaa
     5941 agggaaaaat gtgatcgttc tccatttaga aagtttccag cagtttttga ttaatatgaa
     6001 ggtagacgga caagaagtca caccgttttt aaatagtatt ttccagaatc aagcaacgat
     6061 cagctttgac aatttctttc atgaagtagg acagggaaag acaagtgatg ctgaaaatat
     6121 gttggaaact ggcacatttg gtttacctca aggctcatta ttcacagaac ttgggtcaga
     6181 caatgtcttt caagccgcac cagcgatcct tggacaaaag caaggctata caagcgctgt
     6241 tttccatggg aatgtagcta gtttttggaa tagagatcat gtatataaaa acttaggtta
     6301 tgacaatttc tttgatcgat cttattttga tgaatccgac gaaacgttag gttatgggat
     6361 attagataaa gatttattca gagaatctgc ccaatatctt gagcatctac aacagccgtt
     6421 ttacacgaaa tttctttccg taaccaacca cacgccttat tatacggatg acaagaattt
     6481 cgactttcca tctttgaata ctggcaatag cacagtagat aattatgttc gaacggcaca
     6541 ttatctggat caatcattgg aacaattttt tacgtatttg aaaaaatctg gtatttacca
     6601 aaattcaatc tttgtgattt acggggatca ttttgggatc tcgaatacag ataataaaga
     6661 tttagcaagc gctcttggta aagatccaga tacatgggat gaatttgaca acgcacagat
     6721 gcaacgagtg ccattgatga tccatatgcc tggttatact aaagggacag tcaatcatga
     6781 atacggtgga gaaatcgatg ttttgccgac attactacat ctattgggaa tcgatgataa
     6841 agattatctt cattttggta cagacttatt atcttctcag catgatcaag tagttgcttt
     6901 tcgaaatggg aactttgtga cacccaaata caccgtccta ggaggaaaag cttacaataa
     6961 tcagacagga gaaatcatcg atacgaaagc agcaggaatc gatgaggaaa tctccaaaga
     7021 tcaggaaaaa gtcaagactg cgttaagtct ttcagataag ctgaatcagg aaaatctctt
     7081 gcggttttat gtgccaaacg gatttgagac gattgatccg aaaaaatatg actataaaaa
     7141 tgaagcggaa cgtaatgaag caatcgaaaa gcaaaaaggc gaacattcga caagtgtctt
     7201 ttcaaaaaat ggtaataaaa caacaaccaa cttatatcca gtaacaagaa cagacgttga
     7261 acagcaaaaa aataatcaat aaacaatcaa accaaccttc agtagttaga caacattcta
     7321 atgcttgaag gttggtattt tttatgaaaa aaacattttt ttattttttt cgtgtatttt
     7381 tcctttttaa cgtatctatt tattgaatgt ggaaaaattt aaattaaaaa ggagaactat
     7441 tatgtatgat ggtgaattga tcaagaaact tcgagtcaac aagaagttga cacagtccca
     7501 attagcggaa ggaatctgtt ttaagacttc tctagtcggg attgagacaa attcagtgaa
     7561 aaaaatgtca tttgtaacat tgagatcatt tttagagcga atgaacatga cacttgcaga
     7621 atatgaatgg atgcgaaacc agctggatga accaataaag ttaaagaaaa aaagacgaat
     7681 gctagatcgg gtacaagaag acagctttga tccttataaa gagatcagca ataaccgtag
     7741 gcattttaag aaaacagaag atctgtatta tttggttttg aatcttcaca tggtcgaaaa
     7801 aacagaagta tctgagacag aatttgaatt tttacgttat gaatgccgaa tcattgaaga
     7861 atacttgatg cagataccag actatggtca atttgaacta tctattctag ctgattttcc
     7921 ttggatattc agtgatcggt tcattgagaa caattatttg aaaatcaaaa aaagaatgcg
     7981 acgattagca cagttgaaca atcatgagca gtatctcttt acttttttaa tgaacctgac
     8041 ttcgttttat atagaaaatg gtcggttgaa aaaggcacgt agtgtgaatg aagatatgaa
     8101 acggagctta gcacataaag aaagaagtac agtgatctat gaaacattga tggcagatta
     8161 ttatcaaaga ttgatttctg ctgctttagg ggaagaaaag acgaaagact accaagaatt
     8221 cttcacagtg ctcgaatata tgttaggaaa gaatcaacgg aggtccttag agaaaaaact
     8281 gctggccatt gctgaaccca gcaactagac cagcagttta gaatttgtat cacttcgccg
     8341 ctttttcaga cggtttgttt atatcgaatg gcgggaaaaa gcgggcgatc tcttttaccg
     8401 cagcatcacg ggaatcagaa gcgtgaatga tgttttcagt ccccggcagc gcgtagtctc
     8461 ctcggatcgt acctggaaca gcatcggcag cttttgttgc tccaaccatc ttacggacga
     8521 tatcgatcac ttcctcgccg accagcacaa gataaacgac aggaccggaa gtcatgtaat
     8581 cgatcagttc atcaaaaaaa ctgcgctcag tcaagtgctg ataatgttct tttgctaatt
     8641 caggagtcat agtttcaaac ttcatttcag caatggccag tccctttgct tcaaaacgtt
     8701 ggatgatgct tccgactaaa tggcgtctca cgccgtctgg cttgataata acaagtgttc
     8761 tttccataac agcacctcgt ttcctactat atttctatta tagcgggagg tggaaaatga
     8821 gacaaataaa accgcgtgag acaggaatat aacgtgtgta ttttaaaaaa agtatgaaaa
     8881 acatggaaaa ttgatttgac atcctctttt ttagttgtta tattaacgaa ggaaaaccga
     8941 ataagatttg ttaggtgagg ctcctataca aacataggct actgcccaaa aacgtcgaga
     9001 gacgccaatg ggtagaacag gaattgtcga gataaggctt ttcataaggt agctaaaagc
     9061 aggtatctgc tttttacgtt gtacagtgtc aaaactcaac gatgagatct gagaagcttt
     9121 ttttaatatg ctttttcaaa cctttcgctg agtatttggc gagaggtttt tttgttgccg
     9181 caaatcccca tcggtgaaaa ataaaaagga ggtggtctaa ttggaaaaat aactattcga
     9241 aaaatgattg gaagtcatga attaaaatta cttagtaaag gagatgagaa caaatggatg
     9301 acagtcattc gaaaaacaag gatctagagg aaaggccagg gctattatct atctgttctc
     9361 agcccctttc agttttttct ctatcgatcc gatgaatagc taaagaccaa aagcagcgag
     9421 aacagctgtc ttttggacac aaaggagaga ttgagatgga aaaaaagaaa gatacacaaa
     9481 aaatctatgc agcttttgct ggaaaaacga aacaagaatt atttgaacta ttccatgcat
     9541 cagcaaatgg attgacagaa gaacaaatcg aacgttcaag agaaaattac ggtgaaaatg
     9601 cgatttcata tggcaaaaaa acaccattca ttgtagaaat attgaaagca tacatcacgc
     9661 catttacatt agtgttgatc gcattaggaa ttatttcatt catcacggaa tatgtattag
     9721 cagctcctgg agataaagat cttttcggtg tagccattat cttcactatg gttatcgtca
     9781 gcgggacaat gacgttgatt cagtccgtac gttccaatca agcggctgaa aaactgaaat
     9841 cacttgtgaa agtaacggca gctgtaaaac gtaatggaaa gtacacagag attcctatgg
     9901 aagaaatcgt ctgtggagat cttgttcggc tttcagcagg ggatatgata ccagcggata
     9961 tgcgtttatt gtccgcaaag gatttattca tttcccaagc agcattgaca ggggaaagct
    10021 atccagtaga aaagcatgcg aaagtatgtg aagacgatca gcaaagcgaa acaagttacg
    10081 aaaatcttgt ctttatggga agtaatgtca tcagtggaag tgcggaagga atcattgttt
    10141 ctgttggaaa tgaaacacta tttggtcatg tagcagaaac gctatctgaa aaaccaattc
    10201 aatcaagctt tgaattaggc atccataaaa catccatgct actgatcaaa tttatgtcat
    10261 tgatggctcc agcagttatc gtcatcaatg ggcttacaaa aggggattgg ctagaagctt
    10321 ttctttttgg actatctgta gcagttggtc taacacctga aatgcttcca atgatcgtta
    10381 cgacgaattt ggtcaaagga gcaagtgtga tggccaaaaa aggaacagtg atcaaaaatt
    10441 tgaatgccat ccagaacttt ggtgcaatcg atgtgttatg tacggataaa acggggacat
    10501 tgacacaaga taaaatcatt cttgaatatc atatggattg tgaaggaaat gaagatgatc
    10561 gtgttttgcg ccatgcgtat ctaaatagct actatcaaac agggctgaaa aatttgttag
    10621 atgtggctat cattgatgaa gcaacagaaa ctttagatac agataagatc aattatcaaa
    10681 aagtggatga aattcctttt gattttgaac gtcgtcgtat gagtgttgtg gttgctgatc
    10741 cagctggtaa aacacaaatg atcacaaaag gtgcgattga agaaatgctg aacatttcta
    10801 aatttattga tcttggcggt actgttacac cattaacaaa agaacgaaaa gaagccgtac
    10861 ttgcaaaagt acagtcactt aatgaagatg gcttacgagt gattggtgtc gcacaaaaga
    10921 ccaatccaag cgtcgttgga gaattttcag tcaaagatga gtcagacatg gttttgattg
    10981 gttatcttgc tttccttgat ccaccaaaag aaacaacgaa acaagcgttg aaagctctga
    11041 aagaacatgg tgtagctgta aaagttctga ctggggacaa cgaattagtt acacgttcag
    11101 tgtgccgtca agtaggtcta gagatcaatg aattggttac cggtgagaaa atctcggaaa
    11161 tgagtgatca tgaattagca caagtagctg aagaccatga agtattcgta aaattgaatc
    11221 ctcagcaaaa agcacgtttg acgacagctt tacgtaaaaa tggtcatact gtcggatttt
    11281 taggagatgg tatcaatgac gctcctgcaa tgaaagtggc tgatatcggt atttctgtag
    11341 atacagctgt ggatattgcg aaagaatctg cagacgtgat ccttttagaa aaagatttga
    11401 cgattttgga aagaggcctt ctttctggac gagaaacttt tgggaatatc atgaaatata
    11461 tcaaggctac agcaagttct aattttggca atatgttttc cgtgctgatt gcaagtacgt
    11521 tcctgccatt tttaccaatg ctgcctttac aacttttgtt tttgaatctg atctatgatg
    11581 tttcctgcat ttccttgcca tgggatcgga tggataaaga atatttggac gagccaaaaa
    11641 aatggaatgc atctagtatt ggggaattca tggtttactt tggtccaaca agttcgatat
    11701 ttgatattac tacttacttg ttgatgtatt ttgttatttg tcctgctgta gtgggcggaa
    11761 gtttccatac attggatagt tcacagcgag tgattttcat cgcattattc catgcaggat
    11821 ggtttgtcga atcactatgg acacaaacct tagtgctcca tgcactgcgt acaccgaaag
    11881 taccatttat ccaaagcaac gcaacatttg ccatgtttgt tatcactaca ttaggaatta
    11941 ttgtcggttc tgtccttcct tttacaagtt ttggtgctga attaggcttg atgccacttc
    12001 ctggcaatta ctggttatgg ttatttgcta cagtcatcgc ttatctggcg cttgtgacag
    12061 ctgtgaagaa aatctatatc cgtcgattcc aagaactctt ataaacgaga aggctggttc
    12121 tttgcttctg ttctttttgg accagtcgtg ctaaactatg tgtattgttt tggaaaggat
    12181 taatgtatag atgaaaggtg caatatttga tttagatggt ttgttagttg acacagaaaa
    12241 aacgtatcgt gatggctggc tttggggatt tcagcaattt gatatccata tcccaaaaga
    12301 aatcgttgac gcttggggcg gaaaaaactg gaaacaaagt tttgatattt tagtgaaagc
    12361 agcaggtagc cctgaaaaag tacaggaagt ccgagcgaag cgtgaagaat atttttatca
    12421 acagctgaca agcggagcca tccacttaaa accatatgca aaagaaatat tggcagactt
    12481 aaaagaacgt catatgaaac taggcattgc tacgaccact gttacgaaaa gagcaacaga
    12541 tatcttggat cactttaaat tagctgatta cttggacacg tttacattcg gagacgaagt
    12601 gtcggagaac aagccttcac cgattcctta tctgacagct ttggaacgaa caggattaga
    12661 agctagttca gcctttgcag tggaagattc tttagtagga gcaacagctg cttcacgagc
    12721 aggattaggc gttgttttga tacctgattc cagttttgtc cgaaattata ccgtcgaaga
    12781 aaaggaaaaa ttgaaacttc ttgcggaagg aaaagatctt cgaactgtta ttga
//
LOCUS       DANKAY010000075        12747 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000075 DANKAY010000000
VERSION     DANKAY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PWY98_002064"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PWY98_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2037803.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="PWY98_002065"
     CDS             complement(323..892)
                     /locus_tag="PWY98_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2037804.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="PWY98_002066"
     CDS             1078..1980
                     /locus_tag="PWY98_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2037805.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PWY98_002067"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PWY98_002067"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2037806.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PWY98_002068"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PWY98_002068"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2037807.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="PWY98_002069"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="PWY98_002069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2037808.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="PWY98_002070"
     CDS             8554..8739
                     /locus_tag="PWY98_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2037809.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="PWY98_002071"
     CDS             complement(8801..9997)
                     /locus_tag="PWY98_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2037810.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="PWY98_002072"
     CDS             complement(10167..11603)
                     /locus_tag="PWY98_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2037811.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="PWY98_002073"
     CDS             11896..12705
                     /locus_tag="PWY98_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2037812.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DANKAY010000076        12690 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000076 DANKAY010000000
VERSION     DANKAY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..177)
                     /locus_tag="PWY98_002074"
     CDS             complement(<1..177)
                     /locus_tag="PWY98_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2037813.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
     gene            319..933
                     /locus_tag="PWY98_002075"
     CDS             319..933
                     /locus_tag="PWY98_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2037814.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1122..3002
                     /locus_tag="PWY98_002076"
     CDS             1122..3002
                     /locus_tag="PWY98_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2037815.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3081..3308
                     /locus_tag="PWY98_002077"
     CDS             3081..3308
                     /locus_tag="PWY98_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2037816.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3313..3855
                     /locus_tag="PWY98_002078"
     CDS             3313..3855
                     /locus_tag="PWY98_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2037817.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4082..4798
                     /locus_tag="PWY98_002079"
     CDS             4082..4798
                     /locus_tag="PWY98_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2037818.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4858..6876)
                     /locus_tag="PWY98_002080"
     CDS             complement(4858..6876)
                     /locus_tag="PWY98_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2037819.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6889..7887)
                     /locus_tag="PWY98_002081"
     CDS             complement(6889..7887)
                     /locus_tag="PWY98_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037820.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8085..9326
                     /gene="sstT"
                     /locus_tag="PWY98_002082"
     CDS             8085..9326
                     /gene="sstT"
                     /locus_tag="PWY98_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2037821.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            9473..10108
                     /locus_tag="PWY98_002083"
     CDS             9473..10108
                     /locus_tag="PWY98_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2037822.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10299..11039
                     /gene="truA"
                     /locus_tag="PWY98_002084"
     CDS             10299..11039
                     /gene="truA"
                     /locus_tag="PWY98_002084"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2037823.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11184..12563)
                     /locus_tag="PWY98_002085"
     CDS             complement(11184..12563)
                     /locus_tag="PWY98_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037824.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT         4236 a         2021 c         2487 g         3946 t
ORIGIN      
        1 atcatataga tcatagatat ccttataatg tgtataaaaa gtcgaacgat gtacatctgc
       61 cgtttctgtt agttcgcgta tagtgattgc ttgaatcttt ttatttacta ataatgtgga
      121 tagtgtttca gccaaagctt tttctgtttt tttgacgcgt ctatctgtct ttttcattta
      181 cctatctcct tatccgacat atcaaagcaa agctgtcgct taatcgacaa acttatagat
      241 gattgattat agatagtaga cactgtgtct attataatgt aagtataaca caatcattga
      301 agttgaggag gcgaaatgat gaaactaact tttaaaccct tagaaaagaa agattacaaa
      361 aaagctatct attttgctgt tgaaggaatg aactttgaga gatatatgga aaatgaactt
      421 ctgctgaatc tttacggaag atattttttg tatatggagt tgaatcgagc aactcaaatt
      481 cttgctttgt atgcagagga cgagttggca ggtgttttgc tggcggagat aaaaggagaa
      541 aaaaagaaac atcgttcttt tggacagcaa atatatgtta agatattcga ttttttacaa
      601 aatatatttg ctaaagaaag taaaggagct tacgagacag caaatgaaca aatgcttgca
      661 gcatatttaa agaagcattc gccagatgga gagatcattt ttttagctgc aaatcctgat
      721 ttgaaaacaa aaggcatcgg taccaagttg ctaacagaat ttgaaaaaag agagaaagga
      781 aaagaaatat tcctttttac agatagtggc tgtacgtatc aattctatga acgacgtgga
      841 tttgaaagac taaaggaaaa aattagcaag ataaaaatct tagataaaga aatagatctc
      901 cgatgtatgc tgtacaggaa aatcattcaa tgaaaatttc gatagaaacg acaatagttg
      961 gcaagatcac ttttagtgag tcttcttgct aactattttt tgtagtgtat tctaccaaaa
     1021 gaaaaccgcc tttttgacta aacttgatag tggtcaattt tttatgatgg ataagtatct
     1081 atacttagtc tcgtaagaat gaaaggaaaa gagggagaaa gatgaaatta caaaagtgga
     1141 tccaagagca tcgaaatcat atcaccgcct tatcagctgt gttgattatt tttggtttta
     1201 tcggaaaatt tactggtttt gtcttgttct ataacattgc tctagccata gcttctatta
     1261 ttgcggtcat acctattatg atccatgcgt atcaggcagt gaaagtcaaa gttatctcaa
     1321 ttgatttact cgttactatt gctgtgcttg gtgcctttat gatcggagaa tacaaggagt
     1381 cagctatcgt tacattcttg tttttgtttg gtagctatct ggaacaaaaa acattgacca
     1441 aaacgcggga gtcaatcaag gctttgaccg agatggcccc atctactgct agtgttgtcc
     1501 aagaagatgg aacgactgaa gaagtggaaa tcgatgacgt ggaagaaggc gatatcgtct
     1561 tagtaaaaac tggtggagct attcctgtgg acggacgagt cattgacggg aaagggtatg
     1621 cggatgaagc cgcagtgact ggtgaatcac gacaagtcgt caaaatcagt ggagatgaag
     1681 tcttctctgg aacgatttta gcagatggct acgtgacgat tgaagcaact aaggtcggag
     1741 atgatacgac ctttgccaaa attatcgaat tagtagaaga agcacaagat accaaatccc
     1801 atgcggaaaa attcattgat cgattttctc agtattatac tccaaccgtt ttacttattg
     1861 cgttggttgt tggtctgatt agtcgtgatt ttcgtttagc aatcactgtt ttggttcttg
     1921 gttgtcctgg cgctttagtc attggtgcgc ctgtttcgaa tgtagcagga atcggtaatg
     1981 gagcgaaacg gggcatctta gtcaaaggcg gtgaagcaat ggatactttt tccaaaatcg
     2041 atacttttgt ctttgataaa actggtacat tgaccaaagg aactactgct gtatccacta
     2101 tcaagaacta tacagaggat gaaggggaca gcttagcttt agtggctcga ttagaaacgt
     2161 tatccgatca tcctctgggg cgcgccgtga tccattatgc tcaggagaaa gacattgatt
     2221 ttcaacaatt agcagtcaaa gataatcaga caatcaaggg acaaggaatc acagctgaga
     2281 ttgatggtca tacagtttgt gcgggcaaca ccaaattgat tgccgctcac aaactttctc
     2341 tcacaccaaa gcaagtacaa gatttacatc aattacaaca aacaggcagc tcggtcatta
     2401 ttgtagcaat cgacaaaaaa atcactcaaa tcataggtgt gtcagatgtg attcgaccag
     2461 aagtcgctga acaattagct aagttgaaac aagcaggagc aaaacagtta gtgatgctga
     2521 caggtgataa tcagatgact gctgattatg tagctgatat gttaggcatt gatgaagtcc
     2581 atgctgaatt attaccagac gaaaaggttc aatttgtgaa aaaataccaa gaacaaggat
     2641 tgcgcgttgc ttttattgga gatggaatca atgattctcc ctctttggct gcggctgata
     2701 tcgggattgc tatgggatca ggaacggatg ttgctatcga aacgagtgac gttgtcttga
     2761 tgcaatctaa ttttgaatcg ttggttcatg cctaccgctt ggctaagaaa actgttttaa
     2821 atacccgtga aaatgtagtg attgccattg gtgtcgtctt atttttactc aacggcttat
     2881 ttgccgggtt tatttacatg gctagtggga tgttcgttca cgaagctagt atcttgatcg
     2941 ttatctttaa cgcgatgcgt ttattgtatt ttggacacga aagcaaagaa gaaaaatcgt
     3001 aaaacttgat aagaatcaat ttttttatgg aagctgtaat ctagaataag actatcaaga
     3061 aaaaggaagg aagaactacg atgaaaaaaa ttattatgca attagataca ctagcttgtc
     3121 caagctgtat gcaaaaaatc gaacatgcgg tttctcaaca gcctggggta agcaatacga
     3181 aagttttgtt taatgcgagc aaaatcaaag caaactttga tccagatgaa gtccaccctg
     3241 aacaattagc tgcagttgtg gaaaaattag gttatgaagt ccaatcagca aaagtaaaag
     3301 aggcgtaaat caatgaatat agtggaagaa aaatttgttc aagaaacaaa acaagcagat
     3361 atcgaccatc ataaaccaac tgctggcgcc atggtggggc atgtattagc aaattttaaa
     3421 ttagaagaaa tgaaattgat ccaagcacaa ttgtatgttt tgggtacatc ttcgcaagaa
     3481 ttaagtaccc ttttttcaga atatgctgat caagaagcga gatggttcaa taaaatctct
     3541 catgagttag tgattgaaaa tgaaatcgtt ccgtctactt tagaagaagt catgcgttat
     3601 ggaaaaattt acgaacatgg tcaaaacaaa tatttaccag cctcaacaat gttggaaaac
     3661 tttgcaaaag attttgattt ccaaaatgtc tttatcacac gggcaatcaa attagctgaa
     3721 aaagaagaaa aatatggctt gcaacaattg cttgttgaac ttttaagttt caataaagaa
     3781 atcatctacc aagtacaaag actccaagga aaaactgtcc gacaagattt ggatgaggaa
     3841 gacgatgatg actaaaagtc tatgtttctt caaatacaaa tataaagcgt gaggtaaatt
     3901 ggaaaatcac gaagattaaa ataaaagaag cacccgctga tgtattacag acaacatcag
     3961 cgggtgcttc tcagttttta gaagatttga aagattgatg agaaaatacg aaaaatcaat
     4021 aaaatcacat tgcttatttg atagaataaa gaagtgggag taatcgaaag gagtttttta
     4081 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
     4141 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
     4201 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
     4261 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
     4321 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
     4381 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
     4441 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
     4501 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
     4561 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
     4621 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
     4681 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
     4741 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
     4801 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
     4861 gtcttttatt ttttgtgtcg cccatgttgc actgtactgg ttacctgcaa caagatcata
     4921 ttgtatcaga cgataatctt ctagaatttc tttttgtttc tcagttaaat ttttttcttg
     4981 gataatttga ctatcctctg taacaaagac ctgttttcca ttaaaacgat tagacaagct
     5041 ttctgtagga tcaattgtta atgcaggtag atgattagat acttctgtca acaacgcata
     5101 gtaaggagtt actttgatat tcgcatgctc aagagccata gcaccaaagt tattaggagc
     5161 aacaacttct ttatttaacg tagctgcttg ttcacgacta tacttgttgt tataaatgaa
     5221 ataatctgtc tggtgctgtt ctaatccata ttttttcgaa tcattcccac tatagattgc
     5281 gggtaaatgg tcaccataaa aaacaaaggt aatcggttta tcgattttgt cgatttcttg
     5341 gatgaatttt tttactgctg tatcggtata gtgaaggcct tgcatataag tttgtaattc
     5401 ttttcttcta ttaggaatga cagcagttcc ttcagcagtg tagttcaatt cactatagta
     5461 atcatcataa ggcatatggt tttgcatagt ggataattgg atgaattgtg acgtttccgt
     5521 attgctttgt aaagctttca aggtttcttt gtatgctgaa tcatccgata tgcgtgggct
     5581 ttgttctatt ttatccgtat aagttaattt atccggactg tctaaataat agaacctgtc
     5641 aaaaccaaat ttatcaaaca cttgttttct gcgataaaga gcagctgtaa aaggatggat
     5701 ggctattttt tcatcgaata gatcggtaat atttggactt attttctgac gctccactag
     5761 ctgggtataa ggtgtaacta acgaagcaga caggttactg atactcaagc ctgtcagtcc
     5821 ttcccattcc atgttagctg ttccgccacc ataaccgacg gataacatca atcctcctgt
     5881 tccttctgtt ataatgcttt tgatgtaagg aatagggtct gtaggtactg tcagattagg
     5941 gacacgtgca ggatcactga agctttcact taaattgaga acgatcgtct gattgtttaa
     6001 ccagtcactt cttgtttggt tgatttcatc tgcctttgtg ttgtactttt tcattattcc
     6061 attaattttt tctttcgaat agccagcagg ttcttccatg attttaatgt cgatattgtt
     6121 taggaattga atgactggtc cgttatcttt tacggcgcct tgctggttaa agaaagtctt
     6181 gtttacttta aataaattaa acagcaagaa agaaggcgaa tttttgtgat taatgaaaaa
     6241 gacacccgaa aaacatccta ataatacagc gatactgata aatcgacgtt tccaatgaat
     6301 cttcaaggaa tactgttttt gtagtctacg ttgtacaata atactagtaa tcaataatag
     6361 gagaaggatc aatccgccca cgattaataa gactgggcta accatcgata atagttctga
     6421 caatccggtc agcattttta aatctgaagg aaagacaggc tcttcccgca tttccatttt
     6481 gattactgtc gaaatagtga gcagtaagtt aatgatcaag gtagcagtca aggcgtacca
     6541 aaaacgattg gaaatcaaca atagtaagaa gaaaaatccc ataataatga tgattgtcag
     6601 gatcatgggt gcttgtcgct gagtaaatat ttttaaggca acctggatag tttctttcca
     6661 accttctttt ggttctaaaa cgaaaatctg cacgaaggta aagcagtaaa aaaatgctgc
     6721 aactaaaaaa taacttttat gaacaaaaag ccaatgaatt ctttttttcc aacgctgagt
     6781 taattgttca atcgaatcaa aagcgaagta ttcgatgaaa cgatgaatga aacggatttt
     6841 ttgtatataa aataaaaaga taactaggag ggacactgct aataaatatc aaataaaaaa
     6901 ctgcccgata tttatcagcc atgccttccc tgagtttgga aattcacgtt tcataaatgt
     6961 agatacgcga taagaaacta aagggaaatt acatataact tgagtgacaa tcaaaaatac
     7021 agcaagaaaa tttcctcttc cttttatacc taatttatca gatagcattt ctaaaaataa
     7081 aaacgaagag aaagtataat acattgcgaa gagagaatat ggtttagaaa acctcagcgc
     7141 tgtatctgta tttcctctga tgatgagtga aatttgtgtc atcaaaataa tcaaaccgaa
     7201 taaaacaatc aagctgataa atacttgagg aagcttatag cggacctttt ctttccagtt
     7261 ccattctttc aaaccataac ccattaaaaa taaataaaaa atccataaaa tactctgtcc
     7321 attctgtaat gcaaacgggt ctttattaaa tagagtagag cagccaacta atccaattgt
     7381 taaaaataat agtagatatt taattgttgt tttcgataat ttatttaaaa atccaactgt
     7441 ataaggaata ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact
     7501 gataggtgtt aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa
     7561 aaagatattg caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg
     7621 taaaactttt aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt
     7681 tgcaccaata acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt
     7741 tgcatatgta ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca
     7801 agcgacaaag attgtcagga agccataact ctccttcttt atttttttca agaataaaca
     7861 cctcacaaaa aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat
     7921 aacatcctaa cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag
     7981 caagaaatgg ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc
     8041 ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt
     8101 tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta
     8161 tctttttccc cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga
     8221 aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa
     8281 ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt
     8341 ctgcacttgt agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa
     8401 cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg
     8461 tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg
     8521 ctgtattgtt gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc
     8581 agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga
     8641 ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc
     8701 tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat
     8761 tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag
     8821 aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga
     8881 tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg
     8941 ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac
     9001 attctttagg gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt
     9061 tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat
     9121 gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta
     9181 tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta
     9241 ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt
     9301 gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga
     9361 gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa
     9421 gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt
     9481 tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc
     9541 gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt
     9601 tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat
     9661 ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt
     9721 agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg
     9781 tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat
     9841 cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta
     9901 tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt
     9961 gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt ggccattact
    10021 gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg
    10081 gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga
    10141 tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt
    10201 atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag
    10261 agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa
    10321 tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca
    10381 ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg
    10441 gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa
    10501 acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata
    10561 ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg
    10621 gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc
    10681 acagctttta tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat
    10741 tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag
    10801 tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg
    10861 tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg
    10921 gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac
    10981 atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa
    11041 aatactttga taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg
    11101 tatgaagcaa attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta
    11161 aaaagtcttt cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt
    11221 tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat
    11281 cttgaagcaa ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga
    11341 ggaatagtta attcagttgt gccaaactta gataattttg tttgtactag tttgccatta
    11401 atagaagcca cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta
    11461 ttcgaattat ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt
    11521 tcctggttac ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct
    11581 ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct
    11641 ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat
    11701 attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata
    11761 taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc
    11821 cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg
    11881 gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat
    11941 ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag
    12001 ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat
    12061 tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag
    12121 aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg
    12181 attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc
    12241 atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac
    12301 catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat
    12361 gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg
    12421 gaaaatgtat aaataatcga aaataacagc gaaatataac gattgttttt tatttgtttc
    12481 atagaaaaat aagaaacgac cattgttaaa aaagtgataa aaaaatagta gatattataa
    12541 cttaaaacaa gatttcccat cattttataa aataaaaaag ctggataaag agtaagccat
    12601 ggataaaaaa tattcatcat tgttccatga tgatcgaagt tattgaagtt tactggagaa
    12661 ctccatacat tatctagtcc tatgattcga
//
LOCUS       DANKAY010000077        12601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000077 DANKAY010000000
VERSION     DANKAY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(26..328)
                     /locus_tag="PWY98_002086"
     CDS             complement(26..328)
                     /locus_tag="PWY98_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037825.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="PWY98_002087"
     CDS             complement(353..2446)
                     /locus_tag="PWY98_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2037826.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="PWY98_002088"
     CDS             complement(2935..3699)
                     /locus_tag="PWY98_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2037827.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="PWY98_002089"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="PWY98_002089"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2037828.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="PWY98_002090"
     CDS             complement(4704..5780)
                     /locus_tag="PWY98_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2037829.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="PWY98_002091"
     CDS             complement(5804..6397)
                     /locus_tag="PWY98_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2037830.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="PWY98_002092"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="PWY98_002092"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2037831.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="PWY98_002093"
     CDS             complement(8067..8366)
                     /locus_tag="PWY98_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2037832.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="PWY98_002094"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="PWY98_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2037833.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="PWY98_002095"
     CDS             complement(10121..11032)
                     /locus_tag="PWY98_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2037834.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="PWY98_002096"
     CDS             11152..12009
                     /locus_tag="PWY98_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2037835.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="PWY98_002097"
     CDS             complement(12196..12558)
                     /locus_tag="PWY98_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2037836.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3547 a         2687 c         2200 g         4167 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 a
//
LOCUS       DANKAY010000078        12542 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000078 DANKAY010000000
VERSION     DANKAY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(282..830)
                     /locus_tag="PWY98_002098"
     CDS             complement(282..830)
                     /locus_tag="PWY98_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2037837.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(919..2100)
                     /locus_tag="PWY98_002099"
     CDS             complement(919..2100)
                     /locus_tag="PWY98_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2037838.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(2336..3844)
                     /locus_tag="PWY98_002100"
     CDS             complement(2336..3844)
                     /locus_tag="PWY98_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2037839.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            4003..4194
                     /locus_tag="PWY98_002101"
     CDS             4003..4194
                     /locus_tag="PWY98_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037840.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            4397..5314
                     /locus_tag="PWY98_002102"
     CDS             4397..5314
                     /locus_tag="PWY98_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2037841.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            5307..6380
                     /locus_tag="PWY98_002103"
     CDS             5307..6380
                     /locus_tag="PWY98_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037842.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            6528..7046
                     /locus_tag="PWY98_002104"
     CDS             6528..7046
                     /locus_tag="PWY98_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037843.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            7027..7419
                     /locus_tag="PWY98_002105"
     CDS             7027..7419
                     /locus_tag="PWY98_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2037844.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            7495..8430
                     /locus_tag="PWY98_002106"
     CDS             7495..8430
                     /locus_tag="PWY98_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2037845.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            8563..9342
                     /locus_tag="PWY98_002107"
     CDS             8563..9342
                     /locus_tag="PWY98_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2037846.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            9335..9652
                     /locus_tag="PWY98_002108"
     CDS             9335..9652
                     /locus_tag="PWY98_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2037847.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            9896..11506
                     /locus_tag="PWY98_002109"
     CDS             9896..11506
                     /locus_tag="PWY98_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2037848.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            11663..12511
                     /locus_tag="PWY98_002110"
     CDS             11663..12511
                     /locus_tag="PWY98_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2037849.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         4010 a         2242 c         2442 g         3848 t
ORIGIN      
        1 aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta
       61 acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt
      121 caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat
      181 tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt
      241 cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt
      301 tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg
      361 atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa
      421 atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc
      481 gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt
      541 gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg
      601 gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc
      661 tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt
      721 ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt
      781 gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct
      841 cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta
      901 actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac
      961 taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc
     1021 atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac
     1081 gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa
     1141 ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc
     1201 cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag
     1261 gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa
     1321 agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat
     1381 gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc
     1441 ttctttttct tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa
     1501 gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac
     1561 gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga
     1621 taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga
     1681 agctactcca ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc
     1741 aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga
     1801 aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac
     1861 accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt
     1921 tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac
     1981 aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct
     2041 gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa
     2101 ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat
     2161 ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat
     2221 aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac
     2281 gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt
     2341 ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt
     2401 tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa
     2461 gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata
     2521 aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt
     2581 tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat
     2641 gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg
     2701 tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt
     2761 ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc
     2821 ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt
     2881 cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt
     2941 gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac
     3001 ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt
     3061 tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat
     3121 caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag
     3181 caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt
     3241 aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct
     3301 ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact
     3361 cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga
     3421 tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg
     3481 tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac
     3541 gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga
     3601 tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca
     3661 atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat
     3721 gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc
     3781 gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt
     3841 tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac
     3901 tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata
     3961 gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa
     4021 aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt
     4081 gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact
     4141 cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata
     4201 agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa
     4261 aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat
     4321 aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta
     4381 agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact
     4441 agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa
     4501 agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc
     4561 tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga
     4621 attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca
     4681 tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat
     4741 gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag
     4801 tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc
     4861 ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca
     4921 gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt
     4981 gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga
     5041 gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc
     5101 tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg
     5161 tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg
     5221 attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa
     5281 tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa
     5341 atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta
     5401 atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag
     5461 aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg
     5521 aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg
     5581 aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt
     5641 ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga
     5701 tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata
     5761 ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca
     5821 attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc
     5881 aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca
     5941 atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct
     6001 ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa
     6061 cacttggttt taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc
     6121 ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga
     6181 aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata
     6241 aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat
     6301 ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat
     6361 taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc agataattct
     6421 gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac
     6481 taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata
     6541 aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat
     6601 ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct
     6661 taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg
     6721 ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa
     6781 tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc
     6841 tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc
     6901 ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca
     6961 atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag
     7021 gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg
     7081 acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca
     7141 tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg
     7201 gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa
     7261 gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat
     7321 tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat
     7381 gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa
     7441 gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa
     7501 aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt
     7561 gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc
     7621 atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc
     7681 aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt
     7741 acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt
     7801 gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg
     7861 ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa
     7921 ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata
     7981 ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga
     8041 acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc
     8101 ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc
     8161 ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag
     8221 atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca
     8281 gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt
     8341 gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat
     8401 aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg
     8461 agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa
     8521 tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt
     8581 gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta
     8641 ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg
     8701 ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg
     8761 caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta
     8821 ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt
     8881 tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct
     8941 tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt
     9001 agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat
     9061 tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg
     9121 atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat
     9181 actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag
     9241 gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg
     9301 ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt
     9361 agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac
     9421 tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt
     9481 tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa
     9541 tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc
     9601 gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt
     9661 attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat
     9721 ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc
     9781 ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct tctatgattt
     9841 tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa
     9901 taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt
     9961 aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct
    10021 tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac
    10081 agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa
    10141 cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc
    10201 ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa
    10261 cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat
    10321 tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc
    10381 tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg
    10441 cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt
    10501 cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga
    10561 acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt
    10621 ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca
    10681 aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga
    10741 tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat
    10801 cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa
    10861 gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt
    10921 gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt
    10981 ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt
    11041 tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc
    11101 gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg
    11161 attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt
    11221 tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa
    11281 ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt
    11341 ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt
    11401 ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt
    11461 tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt
    11521 ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc
    11581 caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct
    11641 ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct
    11701 gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga
    11761 tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa
    11821 aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata
    11881 tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat
    11941 ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat
    12001 tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga
    12061 cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga
    12121 taatacctat gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt
    12181 gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc
    12241 tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc
    12301 tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat
    12361 acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc
    12421 aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa
    12481 agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa
    12541 ta
//
LOCUS       DANKAY010000079        12535 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000079 DANKAY010000000
VERSION     DANKAY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            43..1926
                     /locus_tag="PWY98_002111"
     CDS             43..1926
                     /locus_tag="PWY98_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2037850.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2001..2285)
                     /locus_tag="PWY98_002112"
     CDS             complement(2001..2285)
                     /locus_tag="PWY98_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2037851.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2275..3009)
                     /locus_tag="PWY98_002113"
     CDS             complement(2275..3009)
                     /locus_tag="PWY98_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2037852.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3138..3761
                     /locus_tag="PWY98_002114"
     CDS             3138..3761
                     /locus_tag="PWY98_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2037853.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3953..5074
                     /locus_tag="PWY98_002115"
     CDS             3953..5074
                     /locus_tag="PWY98_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2037854.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5081..8209
                     /locus_tag="PWY98_002116"
     CDS             5081..8209
                     /locus_tag="PWY98_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2037855.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8228..11422
                     /locus_tag="PWY98_002117"
     CDS             8228..11422
                     /locus_tag="PWY98_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2037856.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11568..12275)
                     /locus_tag="PWY98_002118"
     CDS             complement(11568..12275)
                     /locus_tag="PWY98_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2037857.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4270 a         2207 c         2645 g         3413 t
ORIGIN      
        1 atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa acaaaaaagg
       61 ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc tgtggtgggt
      121 gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg cgttccgatt
      181 tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg ggaacaaaat
      241 ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta tccggcactt
      301 gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg gctgttaaac
      361 aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg gcaaatcaat
      421 aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca tatctggtct
      481 ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt gttgatggta
      541 tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt cctctccgcc
      601 atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta ttacggtacc
      661 gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg gccaagtacg
      721 cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc tggaggaatc
      781 tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa tttcatctat
      841 tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt gacagcacac
      901 cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat tggcaaacga
      961 agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc aaaggtgact
     1021 gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat tctaatcatc
     1081 agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta tggtcgtaca
     1141 ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa accatgggtt
     1201 gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc accgaaaaac
     1261 gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa aaaattagca
     1321 gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc gatccttgat
     1381 aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact aacggcattt
     1441 ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc aaaagtagtg
     1501 gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga aaaattaaag
     1561 gaacaagatt tgttgaaaga cactgttctg attggactag gaacgaatgg ctcattctcg
     1621 gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata ctgggtcaat
     1681 gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga acagatgacg
     1741 aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga ccatagtgac
     1801 tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta cattcactta
     1861 gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac aaacgtgaca
     1921 gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa gtttttttat
     1981 atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga gtgtgtctgt
     2041 aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc ttttgttgct
     2101 tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct tttttgaaga
     2161 cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga aagatcggtt
     2221 gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata atgttcattt
     2281 gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt aagtgatcaa
     2341 tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa ctaacaaaat
     2401 atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg caattcgata
     2461 gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca tagtaaagcg
     2521 tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg tcgtatgaat
     2581 ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg gcagcccttt
     2641 gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga cagataagct
     2701 gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa gcgcgatact
     2761 acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag ccgcctgggt
     2821 tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa caatcttccc
     2881 ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa aagaaaacac
     2941 tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc gttcgtcttc
     3001 gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata ctatttttat
     3061 taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat gaccataaga
     3121 agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga gtactattag
     3181 ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa gatgagaatt
     3241 acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg gtagctgcag
     3301 cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca gttctgcgat
     3361 ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga ccaagcgtca
     3421 ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg tttcccagtg
     3481 ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga atgtctaaag
     3541 cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg ttcaagcgtc
     3601 agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag aaaacggata
     3661 aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa ttggacaaag
     3721 aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag tttatgcgtg
     3781 ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa aacgagggag
     3841 agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac aggtgtcatg
     3901 taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt gaatgcgttt
     3961 tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact tgattgaaga
     4021 ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag tagaggcaat
     4081 cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg tagaattata
     4141 caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg ctatatcagg
     4201 aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt attcgaacta
     4261 ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa acgtacaatt
     4321 tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag atgacaatat
     4381 ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc tatttgattc
     4441 agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa aatctgattc
     4501 ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac ttacaccgtt
     4561 tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg ataatgctcg
     4621 ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa aaggcgtatg
     4681 gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac ccctgcatga
     4741 tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt atcagtccgt
     4801 caatcgggag aattacatct atatctatct cactgataga acgatcatcc cgaatatgat
     4861 gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa acggtcgagc
     4921 agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa aattagcaga
     4981 agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa aatggctcgg
     5041 agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga aaaaattaca
     5101 acttaaaaat tttggtcctt ttatcaatga gaccgtagat ttttcacgtt tgacagaagc
     5161 accgcttttt ttgatcagcg ggaagacagg agctggcaaa acgacgattt ttgatgggat
     5221 caccttcgca ctttttggtg aaacttccgg aagacttcgc tctggaaaag agatgcgttc
     5281 tttgtttgct acaccagacg aagaaacaag cgtgactttt tcttttgaac atcagcagat
     5341 gagctatgaa atcgaacgaa aaccagaaca agttttagca aaacgaaaag gaaacggaac
     5401 aaaaaaacag gcagccaaag taacgttgac catttttgat gggaaaggaa aagaaataaa
     5461 acaaatcaca aaacgtacag aagcagatca gttgatcaga gaactgatga atctcgatgc
     5521 gaaacagttt tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt ttttggtttc
     5581 atcaagcagt gataaagaga ctgttttacg tcatttattc ggtactcaat ttttccaaca
     5641 gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg atcatttaga
     5701 gcaagagctg catcttctgc aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc
     5761 agggtttgaa gccgttttag cccaatggac aaaccatcag gcaatactcg aaaaacaaat
     5821 cacggaagaa aaagaagctc taacggaact tcaggaaaaa caaaagcaac tagaaaattc
     5881 ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa
     5941 gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa aagaaacgat ggattgggta
     6001 ttatgaattt acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa taaaagttga
     6061 aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga tgactacgca
     6121 gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa
     6181 agaagctgct caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg aatccttacg
     6241 tagtaaaaaa caagacgcag aacaattaac aatttcattg gataaagaag aattgaatgc
     6301 cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa
     6361 tttactggat caggaaaaac tgcaagaaga tcgtcttgct tttggacagt taaaatatct
     6421 agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca
     6481 gataagagaa gaactcaaaa aagcaactga agaaataaca aaaaatagtc agctattgaa
     6541 agaacagaca gaacaagcag aacagctgaa aagccaatgg gcaaagcagc aaattgctcg
     6601 tcttcagctt tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa cggaacaccc
     6661 catacaacaa ttaaaacacg aacagccaaa taaggaagat atcttaaaag tagaacaaca
     6721 actggcagaa gctgagtctg ctgtagaaag aactcagcaa atcattgcca aaagtgaagc
     6781 acatgaaaat aatcttcaac aacaattatc agatttagaa caaaaaatca tggcaacgaa
     6841 tatacaattg gctgaacaag aagaacactt gcaacaggct ttgaaaaagg catttcctga
     6901 gataacagaa caaaaaacaa aaacaactct tgtagtaatc gaacaatctt tagatagacg
     6961 aaaaagcaag atggccaatg caagaataga gctggaagaa ataagacaac aagcagaaaa
     7021 gctcgaacaa caattagctg tctacaaaca gcagcagaca gaaaaaaata atcagctcca
     7081 gcaactgcaa ggagaaatca aaagtttaga acaacaattg caagatacgg atagtaactg
     7141 tttagaagag gagatacgac gacttgaaaa acagttagaa gaagactcga atcaactagc
     7201 tgttgagcag aaagcaggag tgcaattgtt gcaagatctt catacgcttc ggacacagct
     7261 tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag
     7321 aatagctaaa aagttagaaa gccagtcgta tttttcaaca attgaagaaa tctgtgcatt
     7381 tgcacaacag caagcacaat ataaagaaaa ccaacgtaca gttacttctt atcatgaaga
     7441 gcaattagtg acagctgacc ggctgcgtca attagaaaat atccagctaa ctgagacatt
     7501 cccagataca gaagagatca gccaacagtt agaagaaata agacgtatga tcgaaataaa
     7561 gcaagaaaag atatacggtt tacaagaaca aaaaattagt aatcaaaaaa tctacgatga
     7621 atgtctttcg atttatcaca caagccagac gaaattagat gagcttacgc aattacagca
     7681 gctttcgcaa acattcaacg gagaaaatcc taagaagact agtttggaac ggtatgtgct
     7741 tcaggtttat cttcaagaag tgttacaagt agcaaatgat catctccaac gtttaacgaa
     7801 aaatcgttat caatttgagc tagcggatac cattggcagt tatcgaggga aaaccggatt
     7861 agaaatcaat atctatgatg atcaggcagg tatgtctcga agtgcgcata ccctgtctgg
     7921 cggtgagagt tttattgcag cactttcttt agcattagct cttgccgatg tgatccagac
     7981 acaagctggt ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg ggtcattaga
     8041 tgaagaagct ttggaaatgg caatggaagc tttagaaacg atcgaaagtg aaggaagaat
     8101 gatcggcatc attagtcatg taagagaatt gaaagaacga gtattacaac aaataagaat
     8161 tgatacagaa ggtagcggac aaagcaaaat acgctatcaa cttggataaa aagggagatt
     8221 ggtaactatg aaatggagta ttccagaaaa agtcattgca cgtggacgtc aatacttgca
     8281 agaaggccga gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt
     8341 cttgggcagt gaattgtata tggttgattt ggatggtaca gcaaaagaga tagattactg
     8401 ccaatgtccc tattgggagg aacataaata ttgtaagcat acagttgctg tggaacttta
     8461 tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt
     8521 aaaagcaaca agtgagggag aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa
     8581 caatcctcaa gcggtgcaac ctttgattat tgaataccaa gtagaaacga ttgagactaa
     8641 tcagtatcac ccagagcttt ctattttggc actttacttg cgtatcggtt cattggcatc
     8701 accgaaaaaa acatatatcg taaaaaacat ctatgatttt ttacaagctt acacagaaaa
     8761 agaaacttac actgttaata aacagtacca gtttcgtcta gataaacggg cgtttcaaaa
     8821 agaagatcaa aaaatactca cgcatcttgc aggaagtgct cagacgcatc aattattagg
     8881 tacgaacggc ttacaagtca aaggaaaact agataaaaga tatctgcttc tcccagtaga
     8941 acaagcccgc ttcctgttag agcagatgaa tcagactaca cgtttgcatc tgcagataaa
     9001 tgaacaaaac gatcaaggaa tacaattttc cgatcaaaac aaacctttat cattcaaagt
     9061 ggaaaaattt ggagaagatt atttgctgca agcattgaat gattttgatt ttttctttat
     9121 gcattatcag tggggagtta tccaagggac attttattct ctgacgaaat atcaacaaac
     9181 ggtttatcaa acttggaaac aattgattcg ccgattggag aaaccagaag tagtgttcaa
     9241 gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct ctgctgtcag agatcggcga
     9301 cgtagctatt gcccctgcag tagaagagga agtagcagat atccccgtcg aatttatttt
     9361 ctttttccga aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct atggtgaagt
     9421 catctattcg actgacccga agcatgaggt ctcgacagaa caggcttttc aggtgttacg
     9481 tgacacagta gaagagaaac gagtacttga tctattcaga atgtatcgct accagaaaaa
     9541 tgaaactggt tatgaacgtc cattgccaag cggggaagaa ctatatgcct ttttccgcac
     9601 agaactatct gtcttccgtc agtttggtga agtcagactt ggaagaaaat tgcgagagtt
     9661 gtatttagat gcgcataaac atcaaccgaa aatcgaagta accgagaccg gttcatggct
     9721 cgatattcga ttcgatgtga cagggataga ggagcaagaa atcgatcatg tcctgcaaag
     9781 cttgctgaga aatgatgctt tttatacatt agaaaatggt caggttcttt cttttgattc
     9841 agaagaattt caacaaacaa gccaagtgtt gcaacaatta cgggaatcga tacgtacaga
     9901 agaaggaacg atccatgtac ctaaaaatca aggattgatc attcaaaatc aattggaaaa
     9961 aagcaatgct acttttagtg aatcgttcca aacgatggta caagatctca ttcatccaga
    10021 aagataccag gcacagcttc ccaaaggatt gaatgcaacg atgagagatt atcaaaagca
    10081 aggttttcga tggttgaaga tgttaggtca ttatcagttt ggcggtattt tagccgatga
    10141 aatgggactt gggaaaacac tacagacaat tgccttttta ctttcggaaa aagaagaacg
    10201 gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta atttacaact ggcaggcaga
    10261 ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc aatgggaata aaaaagaacg
    10321 tgaagaatta ctggctaaag acacagatat ccgagtgact tcttatgcca gcttaagaca
    10381 ggatctagct aattatcagt cgcaaaaaat tgattatttg attcttgatg aggcacaaat
    10441 ggtcaaaaat agcagtacga aaactgcaca agcattgaga gaattagctg tacctcagcg
    10501 gtttgcactg agtgggacac caatcgagaa taatttagaa gaattgtggt cattgtttgc
    10561 aacgattatg ccaggatttt tcccgactaa aacaaaattc agggaacttg ccgcagaaga
    10621 aattgcgcaa atgattcgac cgtttatttt acggcgagat aaacaaactg tgttgaaaga
    10681 cttgccggaa aaaacagaga tgaatctata ttctgcactt acagaagaac aaaaaactgt
    10741 ttatttagct tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt
    10801 caagaaaaac cggatcggga tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga
    10861 tcctcgtctg tttattgaag attaccaagg cggttccggc aagctcgaac aagtaaaaga
    10921 tctactggta gctgccaaag agaacaaacg aagagttttg ctgttttctc aatttacagg
    10981 gatgctgact atcttgcaag aagagttagc cgagctaggg atctcgacat tttatttaag
    11041 aggcagtacg aaaccacaag atcggttaag tatggtagac gcatttaatg ctggcgaaaa
    11101 agatgttttt ctgatttcct taaaggctgg gggaactgga ttgaatctca cgggagcaga
    11161 tacggtcatc ttgtatgacc tttggtggaa tcctgccatt gaagaacaag ctgctggacg
    11221 tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac
    11281 gattgaggaa cgcatggatt cactccagca agaaaaacgc gaactgttcc aaaaagtgat
    11341 ccaaggtaat gaagaacagt tgacgaaact gactgaagaa gacatccgca tgattttaag
    11401 tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt cagtaatggg taagaaagcc
    11461 tttctaaaga agcgattgtt tatttgattt aaaaaatagc aaggaggctg ggattccatt
    11521 ttatcccagc ttccttgcta tcattttttt atttctattt ttaaaggtta tacaaagatt
    11581 gtcttcattg tctctaacag ctgatcaaaa gaagaaatct cgatatcgca aattggagtc
    11641 tgttcaattt tgcgttctcg atggttgaac caaagcgctt gccagttagc actttttgca
    11701 cctagaacat cattgtcgta attatcgcca acgtataagg tctcttctgg aagcatatga
    11761 aattctttct cagccagctg aaaaatctct ttttctggtt tttggaaacc tgttgcttga
    11821 gaaatgagca tattgcggga aggaatccaa ttgttcaatt gaagttgctt cagttttttt
    11881 gtctgatggt cagtcggccc atttgtgata attcctaatg ggacattctt ttccttcaag
    11941 aaatcgaggg tttttttgac ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa
    12001 attttttgaa ataagaggcc atcttcttcc gtaatatgag ggtagtctaa atcttttagt
    12061 gattggctga tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc cattactttt
    12121 gggaagtttt catcactatg atggcggaac cggatatata gtggatgcat atctgcatct
    12181 gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg gctgctgctg atcatacatc
    12241 gtatcatcaa catcaaacac aactgattta atcaaaattt tacacgcctt tcggttttca
    12301 ttgatttttt cacaaatcct tgactattgt aacagaaaaa attgtctaaa aaagctgata
    12361 aataaaggtt tttcttacta tatttctatt tccttgacag aataaggttt ttatatttca
    12421 gtttttttca ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa ttaatcatta
    12481 tggtagaagt cgattgattt attttaatgc aatctctgtg gaattttcga aagat
//
LOCUS       DANKAY010000080        12460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000080 DANKAY010000000
VERSION     DANKAY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..283)
                     /locus_tag="PWY98_002119"
     CDS             complement(<1..283)
                     /locus_tag="PWY98_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2037858.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKN"
     gene            561..881
                     /locus_tag="PWY98_002120"
     CDS             561..881
                     /locus_tag="PWY98_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037859.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            1333..1533
                     /locus_tag="PWY98_002121"
     CDS             1333..1533
                     /locus_tag="PWY98_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2037860.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1680..4469
                     /locus_tag="PWY98_002122"
     CDS             1680..4469
                     /locus_tag="PWY98_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2037861.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4516..5043
                     /locus_tag="PWY98_002123"
     CDS             4516..5043
                     /locus_tag="PWY98_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2037862.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            5064..6254
                     /locus_tag="PWY98_002124"
     CDS             5064..6254
                     /locus_tag="PWY98_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2037863.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            6294..7592
                     /gene="asnS"
                     /locus_tag="PWY98_002125"
     CDS             6294..7592
                     /gene="asnS"
                     /locus_tag="PWY98_002125"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2037864.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8387..9061
                     /locus_tag="PWY98_002126"
     CDS             8387..9061
                     /locus_tag="PWY98_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2037865.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            9058..9399
                     /locus_tag="PWY98_002127"
     CDS             9058..9399
                     /locus_tag="PWY98_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037866.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9429..9788
                     /locus_tag="PWY98_002128"
     CDS             9429..9788
                     /locus_tag="PWY98_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2037867.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10312..12396
                     /gene="cadA"
                     /locus_tag="PWY98_002129"
     CDS             10312..12396
                     /gene="cadA"
                     /locus_tag="PWY98_002129"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2037868.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4135 a         2192 c         2662 g         3471 t
ORIGIN      
        1 agtttttaca ggtatagcgc tgctttttta agcgcataac caaaggcatg tgattgtatt
       61 gttcaaagcg gacaatcgtt tccttcttcc catttttcac tacaattact ttcccgtttc
      121 catctaccac agtagaacca caatttctac aggtatgggg agcaggtgag aaaacagcat
      181 cgacgatcaa cgtctttttc ttctgaaagg tctcgtaaga gacctctgta atcatcaaat
      241 ctttctctat taatcgcagc atttttttga tagaatcatt catatagcat atcgtcctct
      301 cagttgttta ttttgtcgtg atttaatcat actagagaac gatatgtttt ttaataccta
      361 aaataaaaat ggggctgaag aatcaattct gattcatcag tcccataaat tatagagcct
      421 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
      481 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      541 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      601 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      661 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      721 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      781 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      841 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      901 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      961 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
     1021 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
     1081 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
     1141 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
     1201 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
     1261 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
     1321 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
     1381 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1441 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1501 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1561 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1621 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1681 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1741 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1801 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1861 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1921 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1981 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     2041 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     2101 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     2161 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     2221 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     2281 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     2341 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     2401 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2461 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2521 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2581 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2641 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2701 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2761 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2821 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2881 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2941 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     3001 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     3061 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     3121 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     3181 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     3241 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     3301 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     3361 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3421 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3481 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3541 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3601 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3661 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3721 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3781 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3841 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3901 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3961 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     4021 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     4081 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     4141 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     4201 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     4261 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     4321 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     4381 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4441 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4501 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4561 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4621 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4681 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4741 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4801 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4861 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4921 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4981 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     5041 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     5101 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     5161 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     5221 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     5281 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     5341 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     5401 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5461 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5521 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5581 catcgaatcc gactggaatg atttatagca agaatgaact tcaggcaatc ggtgaatggg
     5641 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5701 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     5761 tcaatggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggga
     5821 attctgagat catcaatgga atgattgctg ttgcttccca gtcaaccagt aatccgacag
     5881 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     5941 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccag
     6001 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     6061 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     6121 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     6181 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     6241 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     6301 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     6361 tcgcaaataa gcgatcaagc ggaaaaatca catttttaca attaagagac ggaacagcct
     6421 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6481 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6541 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6601 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6661 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6721 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6781 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6841 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6901 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6961 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     7021 tagcattctt agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac
     7081 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     7141 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     7201 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     7261 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     7321 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     7381 caacggacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7441 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7501 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7561 tcccacgttt gttgaaccga atttatccat aagaggttgt gcgcctgccg ttcagctccg
     7621 agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt gtgacttgtg
     7681 gccggagagc aacgtttatt taaaaaaaag aactgcgaac tctcgcagtt ctttttttta
     7741 aatacgggat aatggaagtt tccagcagac aagagaagaa agcaaaagaa attgctattt
     7801 gattcacaag gttctataac gaaatcgcaa tcaatcaaac atttacaaag aaggtaattc
     7861 aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat ctagcacctg
     7921 aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag catgcaataa
     7981 tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt atgaccaatc
     8041 tatcccaagc aatctagaag agatattagt aaagggacat ttactagaag tcttagatga
     8101 gcctataaat atatgtttcg atggcgtcat cagtggtctg attgacggaa gcttgggtgt
     8161 gatcgatctc tctggaccaa acaacgaagg aatcaagggg aatccggcac gttaagaaaa
     8221 aaatagcata aaaaactaga cacttgatga gcgttcattc aattgtttga aggaactagg
     8281 ctggtgtcat tttttataca tatattttct ctttcgtctt gatagggctt atgggataat
     8341 ataaggaaga aaatcatcgt tttattcaaa aaggagtttt tgacaaatga aaaaacgtat
     8401 attatttatc ggtgatagca ttacagacgc aggaagatac cgagcagaag caactgatct
     8461 aggtaaaggt tatccacttt tgattgccgg aaaactaatc tcccaatatc caacgatgga
     8521 atttgaatgc atgaaccgtg ggataggagg agacaaaatt gcagatctgc aagtacgctg
     8581 gcaagaagat tgtttagctt tacaacctga ctatgtatcg attttgatcg gaatcaatga
     8641 tacctggcat aatactagcg atcaggctat attcggaact gagaaatctg ctcagcaatt
     8701 tgaatccgtt tatcgaaaat tactaaatga acttcaaaca gcggaaatct gcaacatcat
     8761 cttgatggag ccgtttgtac ttcctataac cgacgaccgt catggctggc gtaaagactt
     8821 agaccctaaa atccaaattg ttcgtaaact tgctaaggaa tatggcgcat tgctggttcc
     8881 tttagatggt atattcaatg ctttagggat tgcttatggt ttttcgaaat atacaagaga
     8941 agatggggtc cacccaacga ttggcggaca tgagatcatt gcacaaagct ggatagatgc
     9001 aattgtcgca gtcgattttt ttagtggaga taaaaatgag cgaaagaaaa gtgaaaaatg
     9061 atggaaataa ggtgggcagt aaaagaagat ttacctcaaa tcaatcagct gattactgat
     9121 catccagaat cattcgtaaa agcaaaaacg gcagctgacc taaatgataa gaataaacta
     9181 ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat ttgcgcaagc ggacgtgcaa
     9241 cagtcagaag aacgattgga tcagcgacca tcaggaaaac taatccaaca cacattagta
     9301 acaaaagaaa atacggaaga aatccaaaaa aaatttgcag aatacttgat gctggaagct
     9361 aaaaaaagaa gaataaaatc aatttttgcc cagttttaat caaaaacagt aatagaagga
     9421 gaaatacaat gagttgttta ggaaatgtca tctggtttat attcggcgga ctaatcggtg
     9481 gtcttagctg gttagcagcg gggatacttt ggtgtatcac gatcataggt atcccaatcg
     9541 gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc ttttggcaaa gaagtaattt
     9601 atcaccagtc tggcatgtca ttgatcgtta atatcatctg gctgatcgtt tcaggcctac
     9661 caattgctct cgcacatgtc gtcagcggca tccttctatg tatcacgatc atagggatac
     9721 cttttgccaa gcaatctttc aaacttgctc gattggcgtt gatgccattc ggtgcagaaa
     9781 ttgtataaaa gagaataata aaggttagat aaagcataga gccaggtggg atacatgtat
     9841 ttcacctgac tctatgcgtt ttcctgtata agttcttcta tgtgactttt acgtctttta
     9901 tttatatggg gttactgttt atggaaagtt gaaataattt tcattaaaga actctttcta
     9961 tatttttgaa agcgtagtgg agcttcgtat atctgggaag gacattttgc agggattgaa
    10021 aaaagatatt gtcctatcga aaaattaatg aatttagaca aattgccagc aacaggcttc
    10081 aaacttagtt gctatcctgt caagatcaag aaggcaagtg cgggttggat aagagcagtg
    10141 gcaatgatcg aagagaaaaa gaaagaataa gccaaaacat ctgtatggaa gaaattttcc
    10201 gatgcagatg tttttcattt tttctttcgg ataccttgac atatgaataa ccattcattt
    10261 ataattagtt atgaagagat attcatatgt tgaggcgagg aggtcattaa aatgaaaagt
    10321 tacagattag aaggtttaga ttgtgcgaac tgtgcaatga aaatcgaaaa aggagttcag
    10381 aaaatcaatg gagtcaagga agcaacggta aacttcactt ctggaaaact aacgattgac
    10441 gcagaggaag atcatttggc tacgattgaa caagaaacaa aaaaagtagt gaaggaatta
    10501 gaaccagatg tcaaagtaac agaaatagat aaggaaaaag tatctgaaca tggaaatgaa
    10561 aaaaagagaa atacgctgtt ccggatttta ttttcccttg caggtatcgc tttattgcta
    10621 ttatttgatt ttaatgaacc aattcgcttg atagggtatt tattgattta tctgctgata
    10681 ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca aaggaaaaat ctttgatgag
    10741 aattttctta tgtctgttgc tacgattggc gctatgttga tcggtgaata tccagaagcc
    10801 gtagcagtga tgctatttta tcaaatcggc gagtatttcc aaggattggc agtcagtcat
    10861 tcaagaaaat cgattcgcga attgatggcg atccgtccag aaacagctca cgtgcagacg
    10921 gcggaaggat tgatgaccgt gaaccctgaa gacgtgctga tcggtcagtt tgtacttgtg
    10981 aagcctggtg aacgtgtacc gttagatgga acgatcatcg aaggagaatc attagtcgat
    11041 acgtcagcac ttacaggaga atcagtccct aaatctgtat atgttggtga aacagtatta
    11101 agcggattta tcaataaaaa taaaccattt ttagttcaag tagaaaaatc ttatgaaaat
    11161 tctactatca gcaaattatt agaattagta gaaaatgcca gcagtaaaaa agcaccagct
    11221 gaaaatttca ttactaaatt cgcgcgctat tacacaccag tcgttgtagg attggccgtt
    11281 ttattagctg ttttacctcc acttgttgtt tctagagcgg cgttttctga atggatctat
    11341 cgagcattga catttttagt gatctcatgt ccatgtgcat tagtgatttc cgtgccactt
    11401 agtttctttg gcggtatcgg aggagcaagt aaaatcggtg tattagtaaa aggttcaaat
    11461 tacttggagt tgcttgcaca aactgagaca gtggtattcg ataaaactgg cacattaacg
    11521 aaaggtgact tttcgatcca gacgatcgat ccaacaggaa ttgatccaaa gaaatttatg
    11581 caatatgttg caagtgcaga acagttttct acacatccga ttgctcaatc tgttttagaa
    11641 ggctacgcag gtccgctttt accaacagct aatatccagg aatttgcagg tgaagggatc
    11701 ttagctgaag ttgatggcaa acaagtatta gtaggtaatc acaaattgat ggaaagattt
    11761 gaaatctctt ttccttcaag tcaagaaatc ggtacattgc tttatctagc gatcgatcaa
    11821 agttacagtg gttatcttgt tattgcagat accttgaaag aagatgccgt agacgcattg
    11881 gttcaattaa aacaggctgg tgtgaaaaac acagtcatgt tgacgggcga ctcgaaaaaa
    11941 attgctgatc atatcgggaa gcaagtaggc gtggacaaga tatatagcga attgcttcca
    12001 gaagataaag tgcagcggtt agaggaaatc ttacaaagta acaataaaaa gaccgcattt
    12061 gtcggagatg gaatcaacga tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg
    12121 ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa
    12181 ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa cattgaaaat cgtcaaacag
    12241 aacattgtct ttgctattgg gataaaaatt ttggtacttt ccttaggtgc ttttggtttt
    12301 gcatcaatgg gagatgcagt atttgcagat gttggggtga cagtattagc tgttttgaat
    12361 gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt
    12421 cctctttttt ttatatcctt ttcttaatga aaaatgtaaa
//
LOCUS       DANKAY010000081        12355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000081 DANKAY010000000
VERSION     DANKAY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="PWY98_002130"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="PWY98_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2037869.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /locus_tag="PWY98_002131"
     CDS             complement(653..1375)
                     /locus_tag="PWY98_002131"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2037870.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /locus_tag="PWY98_002132"
     CDS             complement(1511..2392)
                     /locus_tag="PWY98_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2037871.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="PWY98_002133"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="PWY98_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2037872.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="PWY98_002134"
     CDS             3632..4111
                     /locus_tag="PWY98_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2037873.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="PWY98_002135"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="PWY98_002135"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2037874.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="PWY98_002136"
     CDS             5412..5540
                     /locus_tag="PWY98_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2037875.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="PWY98_002137"
     CDS             5544..6089
                     /locus_tag="PWY98_002137"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2037876.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /locus_tag="PWY98_002138"
     CDS             complement(6543..8234)
                     /locus_tag="PWY98_002138"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2037877.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="PWY98_002139"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="PWY98_002139"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2037878.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="PWY98_002140"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="PWY98_002140"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2037879.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="PWY98_002141"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="PWY98_002141"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2037880.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3397 a         2543 c         2214 g         4201 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc gtaatttatt cattttttat tctgtataca ctttcaaaaa
    12241 aatatttatt cattgaaaaa atttttttga ataaattaaa aaaataaatt atattatcgt
    12301 tgttagcgtt tactatatgt attattgttg ttttttatac acaaatcatt cgctt
//
LOCUS       DANKAY010000082        12341 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000082 DANKAY010000000
VERSION     DANKAY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..154
                     /locus_tag="PWY98_002142"
     CDS             <1..154
                     /locus_tag="PWY98_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2037881.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="PWY98_002143"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="PWY98_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="PWY98_002144"
     CDS             complement(1613..2455)
                     /locus_tag="PWY98_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2037882.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="PWY98_002145"
     CDS             2780..3196
                     /locus_tag="PWY98_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2037883.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="PWY98_002146"
     CDS             3193..3663
                     /locus_tag="PWY98_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037884.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="PWY98_002147"
     CDS             3677..4450
                     /locus_tag="PWY98_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2037885.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="PWY98_002148"
     CDS             4464..5276
                     /locus_tag="PWY98_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2037886.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="PWY98_002149"
     CDS             5330..6295
                     /locus_tag="PWY98_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2037887.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="PWY98_002150"
     CDS             6431..9154
                     /locus_tag="PWY98_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2037888.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="PWY98_002151"
     CDS             9167..9799
                     /locus_tag="PWY98_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2037889.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10004..10231
                     /locus_tag="PWY98_002152"
     CDS             10004..10231
                     /locus_tag="PWY98_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL2037890.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10300..10566
                     /locus_tag="PWY98_002153"
     CDS             10300..10566
                     /locus_tag="PWY98_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2037891.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="PWY98_002154"
     CDS             10556..10825
                     /locus_tag="PWY98_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2037892.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="PWY98_002155"
     CDS             complement(11004..12305)
                     /locus_tag="PWY98_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2037893.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4032 a         1999 c         2422 g         3888 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattca a
//
LOCUS       DANKAY010000083        12276 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000083 DANKAY010000000
VERSION     DANKAY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            3..782
                     /locus_tag="PWY98_002156"
     CDS             3..782
                     /locus_tag="PWY98_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2037894.1"
                     /translation="MQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNRRLPRVAGHKEG
                     METVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFFDTFVPELIKEN
                     VKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEIVTAVQEIAEEV
                     AKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFLLWQIAYSELYF
                     TKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            779..1579
                     /locus_tag="PWY98_002157"
     CDS             779..1579
                     /locus_tag="PWY98_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2037895.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1740..3008
                     /gene="rseP"
                     /locus_tag="PWY98_002158"
     CDS             1740..3008
                     /gene="rseP"
                     /locus_tag="PWY98_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2037896.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3076..4785
                     /locus_tag="PWY98_002159"
     CDS             3076..4785
                     /locus_tag="PWY98_002159"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2037897.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            4997..9355
                     /locus_tag="PWY98_002160"
     CDS             4997..9355
                     /locus_tag="PWY98_002160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2037898.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9500..9973
                     /gene="rimP"
                     /locus_tag="PWY98_002161"
     CDS             9500..9973
                     /gene="rimP"
                     /locus_tag="PWY98_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2037899.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            9996..11171
                     /gene="nusA"
                     /locus_tag="PWY98_002162"
     CDS             9996..11171
                     /gene="nusA"
                     /locus_tag="PWY98_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2037900.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11193..11486
                     /locus_tag="PWY98_002163"
     CDS             11193..11486
                     /locus_tag="PWY98_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2037901.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11483..11794
                     /locus_tag="PWY98_002164"
     CDS             11483..11794
                     /locus_tag="PWY98_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2037902.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11807..>12276
                     /locus_tag="PWY98_002165"
     CDS             11807..>12276
                     /locus_tag="PWY98_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HDL2037903.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNR"
BASE COUNT         4177 a         2146 c         2724 g         3229 t
ORIGIN      
        1 atattcaaga aaaaagcgaa tttcaattta ataccgatgg accaattcct cagcacattg
       61 ccatcatcat ggacggcaat ggtcgttggg cgcaaaaccg tcgtttgcca cgagttgctg
      121 gccataaaga aggcatggaa acagtaaaga aagtcacaaa aaaagcatca aggcttggag
      181 tgaaagtgtt gacgctttat gccttttcga ctgaaaactg gaagcgacca aaagatgaag
      241 taagttttct catgcaacta cctgtcgact tctttgatac gttcgtacca gaattgatta
      301 aagaaaatgt caaagttcat gtcatgggtt atgaaaacgt attgccagaa catacgcaag
      361 atgctgtaag acgtgcaatt gagcaaacaa aaaacaatac tggaatggta ttgaattttg
      421 ctttgaatta tggaagtaga gcagagatcg tgactgctgt ccaagaaatt gccgaagaag
      481 tagcaaaagg agaaatacac gcagaagaaa tcgatgatga attgattgct aagcatttaa
      541 tgacaggttt cttgcctaaa gagttacaag atcctgagct gatgatccgt acaagtggag
      601 aagagcgaat tagtaatttt ctgttatggc agatcgcata tagtgagctg tattttacaa
      661 aagcgctttg gcctgatttt gatggtgcgc atcttgaaga agcaatcgcc tcttatcaaa
      721 atcgagaccg tcgattcggc ggtgtaaaaa aaacagaaga tgagaaaggg gatcagtcat
      781 gaaacaacgt gtgatcacag cagctgtggc gctagtcttg tttattccga ttatatggat
      841 cggaggcata gcggttgagc ttacagcagc aatcttagcg gtagtaggag tttacgagct
      901 gttccggatg aaaggattga cactcttaag ttttgaagga gttttatccg ccgttggtgc
      961 agtgttactt gttttgccaa aagaaagatg gtttttcttt cttccagaga atgtaagtac
     1021 atttatgtta ttttatctta tagttatgat tcttttagga acatcagtta tttctaaaaa
     1081 tacgtatacg attgatgaag caggttttcc tgtgcttgtc agcttgtatg tgggaatagg
     1141 tttccagaat tttgtcaatg cacgaacaga aagcttggct gttttgatat ttggtctatt
     1201 tattgtttgg gcaactgata tcggagcata tatggttggt cgacgttatg gtcagcataa
     1261 gttaagtcct gatatttcgc ccaacaaaac gattgaaggt gcgctcggtg gtatcggtag
     1321 tgctgttgta gtcgcatttc tttatttatt gctataccca gcaaaagaac tattcaatca
     1381 tggtatgttt gtcatgcttc tgatgacgat tattttttca gcagtcggac aatttggtga
     1441 tttggtcgaa tctgcaatca aacgtcatta tgatgtgaaa gactcgggaa atatcttacc
     1501 aggccatggt ggaatcttag accgtttcga cagtttgctg tttgtatttc ctatcatgca
     1561 tttatttggc attatttagt aagtatacct gcgtttcttt gtgaatttca taaaagacgc
     1621 aggtttttat ttttcattat attgatacta ttggtgtctt tggtatacta tggtaaaatt
     1681 atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag aaaaaagcta
     1741 tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt catgagtttg
     1801 gtcatttctt ctttgcgaaa cgatcaggaa tcctcgtccg cgaatttgct atcggtatgg
     1861 gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt cgcctattac
     1921 ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa atggcaccag
     1981 gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata aatttaagta
     2041 agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat ttagaagatg
     2101 agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc taccctgttg
     2161 atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg ccaaaagacg
     2221 tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct ggacccatga
     2281 ataactttat tttggcaatc gtgttattca tcattctggc atttatgcaa ggtggtgttc
     2341 aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg gcagaagcag
     2401 gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat tcatggaatg
     2461 atctgacaac agtcattacg aaaaatccag gtaaaacatt agactttaag atcgaacgag
     2521 aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat ggcgaaaaag
     2581 ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag atcattggag
     2641 ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga tcactattta
     2701 caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg tcttcagaag
     2761 cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca atgaatctcg
     2821 gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc ttgaatattt
     2881 ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg acattagctg
     2941 gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt caacgattct
     3001 ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt ttgaggaaat
     3061 aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg cgaagttcca
     3121 aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat ccgccaagta
     3181 tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa actgaaacgc
     3241 attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc tgccatcttg
     3301 ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa cttgtatcgt
     3361 ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga aacttttact
     3421 gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct ttatcaaatc
     3481 caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg aagagagttt
     3541 atcatgaaag atgcatattc tttccatgca acggaagaaa gtttggacga gacatataaa
     3601 gattacgaaa aagcctatac cgaagtattc aagcgttgtg gcttagaatt tcgctcgatc
     3661 atcggagacg gcggcgcaat gggcgggaaa gactcaaagg aattcatggc gatctcagaa
     3721 atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa cttggagatg
     3781 gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt ggagaaagta
     3841 gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt tgaacctcaa
     3901 aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt tttggtacgt
     3961 ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga ctttttagaa
     4021 gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc tgtcggacca
     4081 gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga tttagcgaat
     4141 gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc agaacgcgat
     4201 ttcactgtgc tttcttatga agatcttcgt tttgtacaag aaggagatcc ttcaccagac
     4261 aacaacggtg ttcttgcctt tactcgagga atcgaaatcg gacatatatt caaaatcggc
     4321 actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg agaaaaattt
     4381 gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat cgtagaacaa
     4441 cattcagatg aacaaggaat caattggcca aaaggaattg cgccatttga tctgcatatc
     4501 gtccaaatga atctaaaaga cgaataccaa acaagcctga cgaatgaact agaagaagcg
     4561 atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc aggagtgaaa
     4621 ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa aaaagcagtg
     4681 gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt acgtaaagaa
     4741 gaattggcga atactttacc aatcctcttg aaccaaggcg aataataagc gttaaaacac
     4801 agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc ttttaaaatt
     4861 ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa aaaacgagta
     4921 tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg tttctcatca
     4981 gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt tggaacaaat
     5041 ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa tggatagtgt
     5101 agtggtccat cgtaaatcgc gtttatggga atttacgcta catttttcca aaattttgcc
     5161 tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag atattgcaca
     5221 agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct tgcaagatta
     5281 ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc aagtattgaa
     5341 aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca gtacaggagc
     5401 tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg tcagttacgg
     5461 attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac gagtattgaa
     5521 actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag cagctgaaag
     5581 tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag ctcttgaagg
     5641 acccatccac atccagctag gacgaaatat tccagccgat gaaccaacga caccaatgat
     5701 cagtatcgtt gaagaagaac gaagagtcac acttgagggg tacgtattcg acaaagaagt
     5761 cagagaacta cgttcaaaac gcaagatctt gactttgaaa atcacagatt atacttcatc
     5821 atttatcgtc aaaaaattct cgaacggcga aaaagatgaa caagtatttg atgcgatcag
     5881 tgtcggtagc tggttaaagg tacgaggaag tattcaagaa gatacgtttg tccgtgattt
     5941 ggtcatgaat gcgcaagata tcatagaagt caaacataca cctcgcaaag attatgctcc
     6001 tgaaggggaa aaacgagtag agcttcatgt tcatagcaat atgagtacga tggatgcaac
     6061 aaacagcatc tctgatcttg tggcacaagc agggaaatgg ggacaccgag ctatcgccat
     6121 tacagaccac ggtggtgcgc aagcctttcc ggaagcacat agtgctggaa aaaaagcagg
     6181 tgtgaaaatc ttatacggcg tggaagcgaa tgtcgtagat gatggtgtcc caattgctta
     6241 taatgatgca catgaagcat tgagtgaagc cacatatgtc gtatttgacg tggaaacgac
     6301 aggattgtct gcagtatatg acacgatcat cgagctagcc gcagtcaaaa tgtataaagg
     6361 aaatgtgatc gaaagctttg atgaatttat cgatccagga catcctttgt cacgaacaac
     6421 ggtagatctg actggtatca cggatggaat ggttcgagga tcaaagtcgg aagaagaagt
     6481 attgcggatg ttccttgaat tttcaaaaga tacaattttg gtagcccaca acgcggcttt
     6541 tgatatgggg ttcttgaata ctagctacgc aaggtatggt attcctgaag cggccaatcc
     6601 agtcattgat acgttggaat tagctagata tctgtatcct caattcaaac gtttcggatt
     6661 aggtgtgtta tctaaaaaat ttggtgtcag cttggaacag catcaccgag caatctatga
     6721 tgctgaagca actggtcact tagcatggat ctttgtgaaa gaggcaatgg acaatcataa
     6781 catgctttat catgatcagc taaatgagca tatcggagaa ggagattctt acaaaagggc
     6841 tcgacctttc catgtaacga ttttagcaaa aaatcaggca ggactgaaag atttgttcaa
     6901 actgatttca atgtcgaatg tcgaatactt cgaacgcgtc ccacgaatac cacgctccca
     6961 attgaaaaag atgcgtgaaa atctattgat cggctcagct tgtgataagg gagaaatatt
     7021 cgaagccatg atgcaaaaag gggtagaaga ggcaagaaac cgagcaaaat tctacgatta
     7081 tattgaagtt atgccaaaag cggtctatgc gcctttgatc gagcaagaat tggtaaaaaa
     7141 tgaacatgat ttggaagaaa ttatccaaaa tttggtagaa atcggaaaaa gtttggataa
     7201 aatcgttgtt gcaacaggta atgtacatta tctgaacgaa gaagatgcca tttaccgaaa
     7261 aattttgatc aattctatgg gcggagcgaa tccgttgaat cgtcatagtt tgccggatgt
     7321 ccatttccga acaaccgatg agatgctaac tgcatttcac tttttagggg aagaaacagc
     7381 gaaagagatc gtagtagaaa atacgaataa aatcgcagat atctgcgaag aagtgattcc
     7441 agtcaaagat gaactttata caccgaagat tccaggttca gaagatgaaa tatcagaatt
     7501 gagttacaca aaagcaaaac aaatgtacgg agatccgtta cctgagatca ttcaaaaacg
     7561 attaaaaaaa gagttgaatt ctattaatgg taatggcttc tcggttattt atctgatcgc
     7621 ccaaaaacta gtgcataaaa gtaatgaaga tggctatcta gtaggttcac gtggctctgt
     7681 tggctctagc tttgttgcga cgatgacagg aatcactgaa gttaatccgt tagcccctca
     7741 ttattattgt ccagagtgcc aatattccga gttttttgaa gatggtacgt atggttctgg
     7801 atttgacatg ccagaaaaac aatgtccaaa atgtggtgca cgcttaaaca aagacggtca
     7861 tgatattcca tttgagacat ttttaggatt ccatggagac aaagtgcccg atattgattt
     7921 gaacttctca ggtgattatc aagcagaagc ccataactat acgaaagtat tgtttggcga
     7981 agactatgtt taccgtgccg gaacgattgg tacagtcgcc gacaaaacag catatggcta
     8041 tgtaaaagga tacgaaaggg acaataatct gcagtttcga agtgcagaag tcgacagatt
     8101 agcaaaaggc gctactggag tcaaacggac aaccggccag catccagggg ggatcatcgt
     8161 tattcccgat tatatggatg tctatgattt tacgccgatc caatatccag cagacgatca
     8221 aaattcggaa tggaaaacga ctcattttga ttttcactcg attcacgata atgtattgaa
     8281 attagatatt ctagggcatg atgacccaac tgttattcgg atgcttcaag atttatcagg
     8341 gatcgatcca caaacgatcc ctacggatga cccggaagtg atgcggatat ttgccggtcc
     8401 tgaagtactt ggagtaagtc aggaacaaat ttattccaaa acagggacat taggtattcc
     8461 agaattcggc actcgttttg ttcgagggat gctagaagaa acgcatccta caacttttgc
     8521 agaactactg caaatttccg gactatctca tggtacagac gtatggttag gaaatgcgga
     8581 agaattgatt cgacgaggag atgcgacact ggcggaagta atcggctgtc gtgatgatat
     8641 tatggtttat ttgatccatg ctggattaga tagcgggatg gcatttaaaa tcatggaaac
     8701 tgttcgtaaa ggacaatgga acaagatccc tgatgaattg cgagaaacct atctatcggc
     8761 gatgaaagaa aataatgtgc ccgactggta tatcgattct tgttcaaaaa tcaaatatat
     8821 gtttccgaaa gcccatgctg cagcttacgt attgatggct cttcgtgtcg cttatttcaa
     8881 ggtttatttc cctattttgt attactgtgc atacttttct gtacgtgcag acgactttga
     8941 tctagtctcc atgtgcaaag gaaaagatgc tgtgaaacaa gcaatgaaag aaatcacgga
     9001 caaaggatta gatgcttccg taaaagaaaa gaatcaactg acagtgctgg agttagccaa
     9061 tgaaatgctg gaacgaggat tcaaatttgg aatgattgat ttgtataaat cggatgccgt
     9121 taactttgtt attgaaggtg atacgttgat cgcaccattt agagctgtcc caagtttggg
     9181 aaccaacgtg gcaaaacaaa tcgttgaagc aagaaaagac ggtccatttt tatccaaaga
     9241 agatcttgcc acccgcggta aagtttcaaa aacattgatc gaatatatga acgacaatgg
     9301 cgtattgaaa gatctaccag atgaaaatca attatcgtta ttcgatatgc tataagcatc
     9361 tgttttcctg tctgcttgta attttaagca gagtatggta tagttcttat aggtaatgat
     9421 actaactgtg agtgagcgga tttttcgctc actcttttta atggaattac gcacttaatt
     9481 ttttaaggag gcgaaacttt tgagtagcgt cgttgaaaca gtcacagaaa tggtgacacc
     9541 gattttagat gaacaaaatt ttgaacttgt cgaagtcgaa tttgtaaaag aaggaaaaag
     9601 ctggttctta cgagtattta ttgacaaaga aggcggtatc gatatcgaag aatgcgcctt
     9661 tgtcagtgag aaactgagtg aaaaacttga tgcagcagat ccagatccga ttcctcaagc
     9721 ttatttcctg gaggtatcct ccccgggagc agagcggcca ttgaaaaaag aaagcgacta
     9781 tgaaaaggca cttggtgatt atgtccatgt ctccttatac caaccagtag atggcgaaaa
     9841 acaatatgaa ggatttcttc aatcatttga tgctgaacag cttacattga aaatacgcat
     9901 aaaaacacga gaaaaagaat tgacttttga ccgaaaaaat attgcaaaag ctcgcttagc
     9961 tatccaattt taatcacgga gaggaatcag aaaaaatgag taaagaaatg ttaaatgcgt
    10021 tagacgcttt agaagctgaa aaagggatct caaaagaaat cgtgatcgat gctttagaag
    10081 cagcattggt ttctgcatac aaacgtcact atggccaagc acaaaacgtc gaagtggaat
    10141 ttgatcagaa aaaaggaaag atccatgtct atgcggtaaa agaagtaacg gaagaagtga
    10201 tggattcaca gttggaagtc tcattgaaag atgcactttt aatcaatcca gcatatgaga
    10261 tcggtgatac gatccgtttt gaagtaacac caaaagattt tgggcggatc gctgcgcaaa
    10321 cggctaaaca agttatttta cagcgtgtac gtgaagcaga aagaacgatt atttataacg
    10381 aattcagtgc ttatgaaaaa gacatcatgc aaggaatcgt cgaacgccag gataaacgtt
    10441 atatttatgt caatcttggt aaaattgagg cagttctttc taaacaagat caaatgccaa
    10501 atgaatttta tcaaccacat gatcgaatca aagtttatgt ttcccgagta gaaaatacgt
    10561 ctaaaggtcc gcaagtattt gttagccgca gccatccaga cctattgcgc cgcttatttg
    10621 agcaagaagt accagaagtc tatgatggaa tcgttgaaat tgtcagcatc gcccgtgaag
    10681 ctggcgatcg ttcaaaagtg gcagttcgct caaatgatcc aaatatcgat gcagtcggta
    10741 catgtgtcgg gccaaaggga caacgagttc aagcaatcgt caatgaatta aaaggcgaaa
    10801 acatggatat cgtcgaatgg aatgaagatc cagctgtatt cattgcaaat gcattgaatc
    10861 ctgcacaagt catggatgtg atcttcgatg agacaaatcc aaaggcctgc acagttgtcg
    10921 ttccagacta ccaattatcg ctagcaatcg gtaaacgcgg acaaaacgca cgtttagctg
    10981 ctaaattgac taaccataaa atcgatatca aatcagaatc tgacatgacg gaattttatg
    11041 aaaatcaaga acaacaaaaa gaaacagaag aactgcatga tgaagcaatc gttcagtcag
    11101 atttaacaga cgatgaatat gaaacaattg cattcaacaa tgaaacggtc gaagaaaaac
    11161 cagaagctta actttagtaa ggaggcaaag agatgaaaca aaggaaaatt cctttacgca
    11221 aatccgtggt ttcaggtgaa atgaaaccta aaaaagagat ggtccgaatc acccgttcaa
    11281 aagaagggga agtatcgatc gatccaactg gaaaaatgcc tggacgagga gcgtatgtct
    11341 cacttgagcc ggaagaagta caacaagctt gggataaaaa aattctcgat cgagtactcg
    11401 aaacaacatt aacagatgag ttttatcaag aacttcttga ctatgtcact caccaaaaag
    11461 cacgtaaaga gttatttgga aaatgaatac agttaatcgg caaaaagcca tgaatctcat
    11521 cggtctagcg atgcgcgcgg gaaaactaat cacgggtgaa gaactgacca ttgctgaaat
    11581 ccgcaaacaa aaagcaaaat tggtgtttgt ggcaaccgat gcaagcgaga atacaagaaa
    11641 aaagataaaa gataagagtt catattatga agttccttgc tttgagctat tttctgaagc
    11701 agaaatcacg cagatgatcg gaaaacctcg caaggtattc ggaataatgg atgccggatt
    11761 tgcgaagaag accaaggagc taattgaagg ttaggaaggt gattgcatgg gcaacaaaag
    11821 aatctatgaa ttagcaaaag aactcaaaaa atctagtaaa gacgtcgtgg aaaaagcaca
    11881 acaacttggc atcgatgtga aaaaccatat gggtgcgatt actagcaatg atgagaagaa
    11941 attgcaacaa gcgttcaaaa acccaagcac gaaacaacaa gctgcacaac aagcaagcag
    12001 aaaaccagcc aatcaacagc cgaacgatca acgtaaaaaa gaaacaacga acaatcaaca
    12061 aaaaaataaa aataaccgca gttatcaaga tcgcggtcaa gggagcggtc aagtgaatca
    12121 aggaaaaaaa ccaacaacaa accaaaataa tacgggaaat accagcagcc aaaatcgtca
    12181 aggaacgagc caaggcggca atcaaaaccg tactaacaat aacaacaaac caaacaataa
    12241 caatcgcggc aaatttaata ataataaccg taatcg
//
LOCUS       DANKAY010000084        12089 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000084 DANKAY010000000
VERSION     DANKAY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            476..727
                     /locus_tag="PWY98_002166"
     CDS             476..727
                     /locus_tag="PWY98_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037904.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="PWY98_002167"
     CDS             761..1039
                     /locus_tag="PWY98_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037905.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1636..1893
                     /locus_tag="PWY98_002168"
     CDS             1636..1893
                     /locus_tag="PWY98_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037906.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2006..2617
                     /locus_tag="PWY98_002169"
     CDS             2006..2617
                     /locus_tag="PWY98_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037907.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2796..3353
                     /locus_tag="PWY98_002170"
     CDS             2796..3353
                     /locus_tag="PWY98_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037908.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4052..4573)
                     /locus_tag="PWY98_002171"
     CDS             complement(4052..4573)
                     /locus_tag="PWY98_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2037909.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4772..8464
                     /gene="nifJ"
                     /locus_tag="PWY98_002172"
     CDS             4772..8464
                     /gene="nifJ"
                     /locus_tag="PWY98_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2037910.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8653..9540
                     /locus_tag="PWY98_002173"
     CDS             8653..9540
                     /locus_tag="PWY98_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2037911.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9537..10706
                     /locus_tag="PWY98_002174"
     CDS             9537..10706
                     /locus_tag="PWY98_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037912.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10730..12085
                     /locus_tag="PWY98_002175"
     CDS             10730..12085
                     /locus_tag="PWY98_002175"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2037913.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         4023 a         2029 c         2657 g         3380 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agtttattga taataatgta tgagatacag tataatgatt taaaaggtaa cgtgttgtga
     1501 ccgaattaaa tgaattaatg cctatttttt atattcctta gtggacaaaa atggagacag
     1561 tgaagttagg agcattgtat cctttgttgc gtagttcaaa aaagtaagcg atttctataa
     1621 tggtcagggt aaacggtgag ggctgtccac gtaaatccta ctattgaaat cacaatgatc
     1681 ggacattcaa aaggtggtgc tgaagcagca aattcagtag ctataaatac gaatgcgatt
     1741 atatttaatc cagcgacagt aaatttatct gcttatgaat tagactcaaa cacatataca
     1801 gcaaacatgg tagcttatgt tgtcgaggga gatgcattaa atgttgtttt tggaggagta
     1861 tacacctatt ggagaattaa tcatgttaga tagacaatat gatgcacctt agtggagaat
     1921 gatatctcgc ataggtaact cagtaaaaaa tcaaggtatg gaagcaatta tctctgcatt
     1981 ggaagatcga tatagatagg cggatatgaa tagtaatagg gggaataaaa tggaatacaa
     2041 tggatcaatg agaattgtac aaattatatt ttggattaca tcgggaattg tgatcatagg
     2101 aggagttttt ttgatgttgc caagcttaat atttcctttc tttgcattaa tctcacctaa
     2161 aatacccgaa ccggaaatca cctatggaga atttccattt aggattgaat acgaactaga
     2221 tggacagatt aacataattg aagatactgt catagctgag tttaacgggt gtgagttcag
     2281 cgcaggttct atgaaaagag aacgaagatg gagaagtaga ttagctagtg gtagagaaga
     2341 tctacctttt ggggacgatc taggaatcta ttttagtcga ggaagtgcgc aatattatat
     2401 gggggaaaat gtacaatcaa tgtcattaaa accacatata gcccttagaa atctcgagga
     2461 aggttttaaa agagaggtag actttatatt aggagaaagt ggatacataa gaactgtact
     2521 taatttcgga gaagcgcaag aagttttgac acaatacgga attaccttaa tcaactggga
     2581 aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga taaatagaaa
     2641 caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac gagtagtagg
     2701 aattcatagc gaatattatt acgaccagta gatattttga tattaaagga gtattacgaa
     2761 ctaatatgtg agaatgtaga ggtgatgtgc gaataatgag aaatttatca aatagaaaca
     2821 aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat gcggttttaa
     2881 gtagaataat attgggtcct aagccaccaa gaccagaaat tacccgaggg gagtttgatt
     2941 ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca attgttgcat
     3001 tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg tggcgtctac
     3061 atttagcaag tgatagacga tcacgaaata tcttattaga tgaattagag gatggtcgaa
     3121 gaatatatta tgtcccagaa agtgctaact attctatggg agatgtacag aaaaaaagag
     3181 agcctaatcc taattggtat ccatttaatg gcgtaacaat agaatatcca cgcaataaaa
     3241 cacctgaaat aggtgcaaaa tttataagtg gtttagaaga tctgtacgat aggtttggaa
     3301 tccgtcttat tagttgggaa cacgatccac caatagaaaa tcgctttgaa taatcgaaat
     3361 ctactaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
     3421 aaaccattga aatactaggc tgtgatcagc aaaaatgaat ctgaataaaa aagagagcaa
     3481 ctattagctg agaggagtta atagttgtta gctttatacg aatcaaagtg gcaaatacag
     3541 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     3601 gaaacaactt gtttaaaata aacgacctag ctcaacaaat gaccatctaa tcaactaaca
     3661 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     3721 ttgggatact agtgagaatc accgtacata aatattactc gttaagggaa attgagaaat
     3781 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     3841 tggtggagat aatttgctta gcttgtcata agaaatgaga taatctggca gatgcaagct
     3901 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     3961 aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4021 tctttacttt tgtcccacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     4081 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtt ggaacaaatc
     4141 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     4201 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     4261 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttgg ccatcgctga
     4321 ttgtgagata ccggtgcatc acgttccctc gctgtaagct ttctttttct ttatcgaaca
     4381 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     4441 cttcaaacca ttgtcggcaa tacggacaag ctaagttgat gaattcgacc atttttttag
     4501 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     4561 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     4621 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gatacatgta
     4681 aaatattttt ataaataaaa gaaaaaaatg gcatgtttta cgttataatg gaacagataa
     4741 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
     4801 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
     4861 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt
     4921 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
     4981 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct
     5041 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt
     5101 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
     5161 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
     5221 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt
     5281 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
     5341 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
     5401 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
     5461 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
     5521 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
     5581 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
     5641 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
     5701 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
     5761 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
     5821 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
     5881 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
     5941 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
     6001 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
     6061 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
     6121 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
     6181 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
     6241 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
     6301 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
     6361 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
     6421 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
     6481 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
     6541 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
     6601 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
     6661 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
     6721 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
     6781 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
     6841 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
     6901 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
     6961 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
     7021 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
     7081 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
     7141 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
     7201 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
     7261 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
     7321 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
     7381 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
     7441 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
     7501 ggaagactgg ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact
     7561 gcaagctgcg atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag
     7621 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
     7681 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt
     7741 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
     7801 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
     7861 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
     7921 aacgatcaag gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta
     7981 tacgccttgt atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa
     8041 agaagctgtt tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa
     8101 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
     8161 catgaggaaa caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc
     8221 tttatttgaa aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac
     8281 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
     8341 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag
     8401 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     8461 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     8521 aataaaacgc atacttgcaa ttttcttgaa aattatgcgt ttttctcaag aaaaatacga
     8581 gcatatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     8641 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     8701 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     8761 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     8821 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     8881 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     8941 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     9001 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     9061 tatatgtcca agcgaaaaat cggtgcgttg atcactatcg aacgtcatac tggattagat
     9121 gagtatattg agacagggat tgctttagat gcagatatca ctggggagct gctgatcaat
     9181 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     9241 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgatccctaa agaattcggg
     9301 actcgacacc gggcagctgt cggtataagc gaagtcagtg acgcgattac catcgttgtc
     9361 tctgaagaaa ccggggacgt cagcatcacg ttagataatg aactgatggc tggcttatcc
     9421 cagcaagaat atttggcaat cttgcgtaga gaactaattg ctgaagagaa aacaaaaaac
     9481 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     9541 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     9601 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     9661 ataatcaaac agttacgggg gtgccgatca agatttctta tgattcaaag cgctactata
     9721 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     9781 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     9841 gcgaaggaac acatgaagtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     9901 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     9961 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
    10021 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
    10081 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
    10141 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
    10201 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
    10261 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
    10321 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
    10381 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
    10441 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
    10501 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
    10561 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
    10621 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
    10681 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
    10741 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
    10801 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
    10861 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
    10921 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
    10981 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
    11041 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
    11101 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
    11161 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
    11221 attccttgta caaacgatta acggtgacct ttccggtttg accatttgtg tcgatgcagc
    11281 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
    11341 tacaatgggc acaaatccag acgggttaaa tatcaatgac ggcgtaggct ctacccatcc
    11401 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
    11461 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
    11521 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    11581 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    11641 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    11701 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    11761 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    11821 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgcg taagtgataa
    11881 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    11941 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    12001 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    12061 caaagaagaa atcggactgg attaagaga
//
LOCUS       DANKAY010000085        11341 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000085 DANKAY010000000
VERSION     DANKAY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..615)
                     /locus_tag="PWY98_002176"
     CDS             complement(<1..615)
                     /locus_tag="PWY98_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2037914.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQ"
     gene            complement(635..1177)
                     /locus_tag="PWY98_002177"
     CDS             complement(635..1177)
                     /locus_tag="PWY98_002177"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2037915.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(1309..2313)
                     /locus_tag="PWY98_002178"
     CDS             complement(1309..2313)
                     /locus_tag="PWY98_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2037916.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(2310..3299)
                     /locus_tag="PWY98_002179"
     CDS             complement(2310..3299)
                     /locus_tag="PWY98_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2037917.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(3296..4102)
                     /locus_tag="PWY98_002180"
     CDS             complement(3296..4102)
                     /locus_tag="PWY98_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037918.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            4268..5224
                     /locus_tag="PWY98_002181"
     CDS             4268..5224
                     /locus_tag="PWY98_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2037919.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(5301..5819)
                     /locus_tag="PWY98_002182"
     CDS             complement(5301..5819)
                     /locus_tag="PWY98_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2037920.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5907..6056)
                     /gene="rpmG"
                     /locus_tag="PWY98_002183"
     CDS             complement(5907..6056)
                     /gene="rpmG"
                     /locus_tag="PWY98_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2037921.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            6284..6730
                     /locus_tag="PWY98_002184"
     CDS             6284..6730
                     /locus_tag="PWY98_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2037922.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(6925..8820)
                     /gene="asnB"
                     /locus_tag="PWY98_002185"
     CDS             complement(6925..8820)
                     /gene="asnB"
                     /locus_tag="PWY98_002185"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2037923.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(9145..10119)
                     /gene="bsh"
                     /locus_tag="PWY98_002186"
     CDS             complement(9145..10119)
                     /gene="bsh"
                     /locus_tag="PWY98_002186"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2037924.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(10453..10540)
                     /locus_tag="PWY98_002187"
     tRNA            complement(10453..10540)
                     /locus_tag="PWY98_002187"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10506..10508),aa:Ser,seq:cga)
     gene            complement(10685..11251)
                     /locus_tag="PWY98_002188"
     CDS             complement(10685..11251)
                     /locus_tag="PWY98_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2037925.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         3631 a         2334 c         1920 g         3456 t
ORIGIN      
        1 ttggtgtatc gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa
       61 tccgccaatt agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa
      121 aacaaatgtc aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt
      181 atgtttaaaa tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact
      241 tgcaccacca gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat
      301 cccgctgaac atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg
      361 ccccataaaa tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa
      421 gattggtgtc actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc
      481 ggcataataa gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg
      541 tatccctaaa aaagcatagg tccataacgg tcgtcttaac catacacgca attttatttg
      601 ctgttgataa ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct
      661 tctgatcaaa gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac
      721 atgttttttt atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc
      781 gataaccggc tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca
      841 aatagagctt ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact
      901 gttcatccat ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt
      961 tcttttgttc tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg
     1021 ataaagtcgt ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat
     1081 agattcgctg ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga
     1141 ttcctttccg gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat
     1201 aaaatggtat aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag
     1261 aataaaaaga agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag
     1321 cgcgtgtttt tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat
     1381 caaaattcct gtaggtactt ccccacttag tagaatcact ctagcaacca tatctgcact
     1441 aagcagaaaa atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg
     1501 tacgactagc ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac
     1561 cgctacacta ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc
     1621 cacgttaagg cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg
     1681 tgcataaagt cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata
     1741 aggccagctc gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc
     1801 cttagataat tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat
     1861 accggacaat aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata
     1921 gacacagcaa gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag
     1981 cgaatagtgc gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat
     2041 gcccaatata ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga
     2101 aacgccaaga caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct
     2161 aacaatcagc gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag
     2221 gtaaataaaa ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag
     2281 caagaaatag aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt
     2341 aaaaacggaa cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg
     2401 atcatcctcg ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca
     2461 ggtagaacat aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc
     2521 ccaataaatg aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga
     2581 cagaaaacaa tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca
     2641 ctcaaatgaa tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata
     2701 tataaaggaa acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac
     2761 caaaaagcaa gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta
     2821 aaaaaagaac taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc
     2881 gaaaactgca aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt
     2941 agaaacacag aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac
     3001 gaagcacccg cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcactcct
     3061 tgcatcaatg caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga
     3121 ggcaaacgaa tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac
     3181 gcctgccaaa aatctgcttt tcccacagtc gctgcgccat ttgaaagagc aacaaaaaaa
     3241 ataatcaaaa gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata
     3301 attgatcctc ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc
     3361 ttggaagcgt gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga
     3421 tcacttcttc aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat
     3481 ctgaaaaacg tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca
     3541 tttgattgat ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg
     3601 ttgtcggttc atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga
     3661 cacgctgggt ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac
     3721 ctgtagcatc caacgcccac tcgattgctt catgatcttg cgtgtccatg ccactgaaca
     3781 gtttttgata agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt
     3841 gttcactagt ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag
     3901 agatatcaga gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac
     3961 ttttcaataa agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt
     4021 caggaatcgt aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt
     4081 cagctgctct taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg
     4141 acgctgtttc aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg
     4201 acagcgttcg aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag
     4261 gaaagaaatg aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg
     4321 agtactgagc gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac
     4381 gacttcatcc aatagtactc atacagtcac agatacactt ggacataaag tagagattcc
     4441 aaacgcgccc aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga
     4501 aaaacctatc gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga
     4561 tttaaaggat gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga
     4621 gcctgatttg ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata
     4681 tagcaaaatc gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca
     4741 actaaaagat gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaga tcatttccga
     4801 ttatcaaaaa tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac
     4861 agctgcagtg ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag
     4921 tggaagaatc gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc
     4981 caaaaaagcc acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc
     5041 tgactacatc atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg
     5101 gaagaatctt aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg
     5161 gctatataac ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa
     5221 ttgattttaa ctaaaaaaag catcttcacg attagtcgtg atatgctttt tttcttatta
     5281 acaaactaga cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc
     5341 catttttgaa aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct
     5401 ataagggcga agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta
     5461 tcgatcaatg ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta
     5521 ccacccgaaa catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc
     5581 gaatgcatta cttccgcatt ttctttcgtg cgaaaatttg gctgcgttgt caacacgatg
     5641 atatggcgat aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact
     5701 aaaattcgat ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt
     5761 aacaaaccat ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata
     5821 ttatccgctc cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca
     5881 gcaaggaatt gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt
     5941 tcgcgtgggc aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt
     6001 gttagatagt tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt
     6061 ttccctccca tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat
     6121 gattttttct agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata
     6181 aatacaacgg ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac
     6241 ttgtttatat agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa
     6301 ggactttcct gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat
     6361 cgcaaatgca ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga
     6421 tcaagcaaaa aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga
     6481 aaaaaatcca agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga
     6541 ttcagatgtg atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa
     6601 aaaggcaagc aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc
     6661 acaaaacaaa gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat
     6721 cgattattga acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt
     6781 cgaaacaatg gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg
     6841 aagagctgtt ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc
     6901 ttttttattg atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg
     6961 aatcgtttca tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat
     7021 ttttctttgc tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc
     7081 ttgatcaaaa aattcttttg caaaatctgc ttcgaacaat gctttgactt gttcgtagcc
     7141 gtgttcttct ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga
     7201 ccatttttcc ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg
     7261 attcaataaa aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa
     7321 aggtactcgg acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc
     7381 ttttggcatc cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc
     7441 ttctgttttc tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt
     7501 tggtgttaac acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata
     7561 ttcttttgca ggagcaagtg attcatataa atggatccgt ccatggaaat ttttcatttt
     7621 tccgatttta cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc
     7681 aatcacacgg atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag
     7741 ttcatccgcc ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa
     7801 aaagtacaaa ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa
     7861 agggaatgct ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa
     7921 tagttcggtt agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat
     7981 tgagaaagaa tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga
     8041 taagaaagaa ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt
     8101 ttcgtcgatt ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc
     8161 atcccaatat tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg
     8221 caaacgatag acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat
     8281 ataaggtttc aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact
     8341 tttgatttct gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat
     8401 cccaaagtgg tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc
     8461 aaacattccg cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag
     8521 gacttctgta tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa
     8581 aggctggtaa ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta
     8641 aataggctga gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac
     8701 atgtttatcg atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat
     8761 tgtattaata attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat
     8821 ttaactttca catcctaata taaaattact gtttatttca ttcatatgca tcttatcata
     8881 gaataaatag cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg
     8941 aaagtttctg tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt
     9001 tgaggctacc ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa
     9061 ccagcaaaaa aagacacgac ctaaagatcg tgtctaatta tgactgtatc agaaaatatg
     9121 ataccaaacg gatctattca ttaattaatt gacatagttt atctgttgct cttttatgac
     9181 tggataactg accaattcat gacgatccaa gtcttcttta ttcatattta cagcagtaat
     9241 ttgactgtct tcatacgtac gataataata aatcccttta tcaacgttac agcaagaaga
     9301 ataaatcgta tattcgtatt ttctttctcc agtatcgcat actccttttt gttgctcgac
     9361 tgagcctagt atatgaaaaa attgactgat actttcagat tctgaatcac ctgatgcagc
     9421 attcatttta gtaaatgtgg cttttacaaa tcgagaaaca gaagagagat ctccaggtaa
     9481 gcccaatcct cccatcccac gactataaac attcaagctg atctggtttg aaaaatgatt
     9541 ttctggtgtt ccatttgata gcgagcgata attgttcaga ttaaataatt gataatcaaa
     9601 tggcggatta tttgtcaaca caccgacagg attatcatat aaatgaagtc catctttcat
     9661 actttcaatt actatcgatt tttctttatc agctaataac cagtgtaaag gtgacaaggg
     9721 tagtttttca ctaaaatcga tattgactag attgatagta gccaataatt ctttagcttc
     9781 gtctatggtt gagcattgcc ctagtatcca tggaataaac tcaaatggtg caacgttatc
     9841 ttttccttct tgtaattctt tataatcagc attgccagga aaattcagtc ctgccatact
     9901 caaccctttt tcatttgtag cctcataata gagtggatag ttggataccc cagctgctat
     9961 tcctatcatt gcataatgtt tatctaaatt ttttaccttt ctaaaatcga aacgataatt
    10021 tcttggagta atagtgacca cttcattata agacatctca taatcaaaat tccttccaaa
    10081 ataatggtct ttcgttgcat aagtgattgc ggtacacatc gtttcccctc ctaaaacaaa
    10141 tcgtttttac acttttatct tacatctagt atttttttta tcaaaaaaag gccaaagaag
    10201 attgatttac tagcatttat aaaaaatagt actcaggggg aatcaaaaaa tattgtaggt
    10261 taaaagtaca acaataaggg gctgtgacaa aagtcttgtc acagcccctt attccgaaca
    10321 aacggtgttc aaaagcttgc atctgcggta ataagcccaa tcaaacgaaa aaataggaaa
    10381 agctattttt gtttggccgt tcttattact tgatgcagga cagcttcttc acaacctcta
    10441 atctttgata taaggaaggt acaggatttg aacctgcgcg ccggtattag ccggttcgcc
    10501 ggatttcgag tccggtgcat taccactctg ccaaccttcc gtaagtacta tttttaaact
    10561 tttttcacga aatgtcaagc ctatttctaa tagataaata acagaaatcg ctttttttag
    10621 caaaaaagtc cattgacagt ttgcagccaa cggacttacc gatcaaattc atactgatag
    10681 aacactatct gattatttca ggattatcta atgctaaaat cgagtcaatt gtccgtttga
    10741 tcagtgcaat gatttgttct tctgttggat gcatgatgtt tgatgacatc aatgacgcta
    10801 agccagtcac tgctacccat gtaccagtat gcagagcatc gacatattca tccgataatt
    10861 gattgtatct ttcatcttct ttaactattt cacggaaata attgtatgaa aaatcatgca
    10921 ttttctttcc gtccccatgt tcatcaatgt acaatgccat gtaaagacta tgatgctttt
    10981 ttgcaaagta gatatagttc aaaccgagat caatgatttt atcaccagta cgttcaatag
    11041 ggaaaacatc ttcttttagt tgttcaaaga tagaatccag caacgtattt tttaaatctt
    11101 ccatgttttt gaattccaga taaatcggct gtgtcgaaat ccccatcttt ttagcaatat
    11161 ttctcgcagt aaatccttta aacccttcct ttgcaacaac atcataagca gcttttagaa
    11221 tttgatcctt ggtatagact tttcttctca ttaaaactcc cccacttctt tactgcagta
    11281 tatgttgttt tattgtaccc tatttttagc attttttgca aacattttac tcaaaacgtt
    11341 a
//
LOCUS       DANKAY010000086        11198 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000086 DANKAY010000000
VERSION     DANKAY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..133)
                     /locus_tag="PWY98_002189"
     CDS             complement(<1..133)
                     /locus_tag="PWY98_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2037926.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     "
     gene            325..678
                     /locus_tag="PWY98_002190"
     CDS             325..678
                     /locus_tag="PWY98_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2037927.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(752..1408)
                     /locus_tag="PWY98_002191"
     CDS             complement(752..1408)
                     /locus_tag="PWY98_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2037928.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            1634..3568
                     /gene="abc-f"
                     /locus_tag="PWY98_002192"
     CDS             1634..3568
                     /gene="abc-f"
                     /locus_tag="PWY98_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDL2037929.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDLPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(3654..4877)
                     /locus_tag="PWY98_002193"
     CDS             complement(3654..4877)
                     /locus_tag="PWY98_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2037930.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(4871..6769)
                     /locus_tag="PWY98_002194"
     CDS             complement(4871..6769)
                     /locus_tag="PWY98_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2037931.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(6763..7881)
                     /locus_tag="PWY98_002195"
     CDS             complement(6763..7881)
                     /locus_tag="PWY98_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2037932.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(7964..8797)
                     /locus_tag="PWY98_002196"
     CDS             complement(7964..8797)
                     /locus_tag="PWY98_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2037933.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(8790..9707)
                     /locus_tag="PWY98_002197"
     CDS             complement(8790..9707)
                     /locus_tag="PWY98_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2037934.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(9934..11058)
                     /locus_tag="PWY98_002198"
     CDS             complement(9934..11058)
                     /locus_tag="PWY98_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2037935.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         3482 a         2236 c         1834 g         3646 t
ORIGIN      
        1 gttctggggt caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag
       61 gtaatgcttc aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa
      121 taaaagaaaa catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg
      181 cttttattca aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca
      241 aagcacttca attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact
      301 tctatcataa aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac
      361 atactgcaac agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt
      421 cacgctgttc gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat
      481 ttggatgcat ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt
      541 gtcctgattt ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa
      601 aaaatccgtt atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa
      661 atcttccgaa caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt
      721 ttgccttcat ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat
      781 ttgcgtcaga atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt
      841 ggatcaatac atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga
      901 ttcctgcttc cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa
      961 ttgctacttc gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg
     1021 ggataccgtc tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac
     1081 gtatgctatt actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac
     1141 cgatcagcgc aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa
     1201 gatcttctac atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc
     1261 gacggatcgt tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt
     1321 tctttccagc attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg
     1381 tcgcctttgg aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc
     1441 tttgatttct ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt
     1501 gtgaaatttt attctttatt catttattta tcatttctgc aaatctcgca gtataatgga
     1561 atttatggta gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag
     1621 aaaagaggat tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga
     1681 agtcttattt gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg
     1741 cagaaatggc gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga
     1801 tgaaggaaag atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg
     1861 acttgattcc aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca
     1921 aatggaagag cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc
     1981 tgcttatagt tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa
     2041 cggctatggt tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc
     2101 attttatgat cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc
     2161 tcgcatgctt ttgcaacacc ctgacatttt gattctagac gaaccaacga accatttaga
     2221 tatcgagaca ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat
     2281 tgtttcccat gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag
     2341 aagaaaaatg cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca
     2401 gttagcaagt gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga
     2461 cttcgttgct cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa
     2521 gacattagaa aaaatggacc ggctggatct tcctcaaggg gatgaaaaac aagcaaactt
     2581 tctattcagt atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat
     2641 cggatatgat aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc
     2701 gattgctcta gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca
     2761 ccagatccct tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta
     2821 cgatcaaggt caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga
     2881 gcatcctacc actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg
     2941 cgaagatgta gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt
     3001 agccaagtta gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt
     3061 agatattgac aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct
     3121 ttttgtttcc catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc
     3181 tgaaaatggc agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga
     3241 agaagaagaa atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac
     3301 gaacaaatct gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg
     3361 aaaaattact caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga
     3421 acaagaaatg gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca
     3481 agatttagaa gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag
     3541 cttggaatta gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg
     3601 ttttggtttt tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa
     3661 taagaagacc aattactagt cattctcact aaagcttcta aataatagta atcaccccaa
     3721 atacaaggtg tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag
     3781 ccatcttctt cttccgttgt agcatatctt tctttcaatt gtcgcataat tttccaaaca
     3841 gcagactggt ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat
     3901 aatccacaaa ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca
     3961 aagatcaaat cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct
     4021 aatattttgc ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa
     4081 ccgtaaatcc cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc
     4141 gtacttccat atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa
     4201 ccatcttctc ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta
     4261 tatttttgat ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa
     4321 tccatgatca ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact
     4381 cctgccttct cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc
     4441 atttttcgat ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca
     4501 agatcgtgat gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct
     4561 ttttcagctg tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt
     4621 cctgtccaaa atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata
     4681 ggacactcgc ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg
     4741 tgtctgcgac aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat
     4801 tcatttatcc atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc
     4861 ccaccagcct ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc
     4921 cggttgtccc atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc
     4981 gatcttgatg agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct
     5041 ctaatccagg aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa
     5101 tagctgtgga ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag
     5161 cttcactcac cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag
     5221 aacattcaat agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt
     5281 gtacgcgaat ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag
     5341 taggttccca tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc
     5401 gaacgatgaa ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg
     5461 tgtccgctcg ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg
     5521 ctcgcggtat cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg
     5581 tcaatgtatg gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat
     5641 catgttgtat gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag
     5701 gcatttcttt agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct
     5761 tattcagcca taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt
     5821 aggtataacc aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc
     5881 ttatattccg attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc
     5941 acaacatagc tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag
     6001 gcacatttgc accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc
     6061 ggttcttata tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat
     6121 aataatgaaa tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc
     6181 agccattttg aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt
     6241 taactggata accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat
     6301 taccatctgc acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt
     6361 ctcctgtatc taattttttc cataataatt gttcatgatg atataacgct aataacattg
     6421 cgggcatttc taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg
     6481 aaggatctgt tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt
     6541 ggtcaattgc gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt
     6601 tatttccata tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa
     6661 acggctggag tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta
     6721 atagtggtac tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc
     6781 cgactgttct aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac
     6841 attataagtc agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc
     6901 catattccaa tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg
     6961 atacattttt gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc
     7021 gcatactcca gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat
     7081 ataaccagtt ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag
     7141 atagtacgga ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt
     7201 tcctctgctc caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt
     7261 ccctgattct gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata
     7321 cttagctgtt gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc
     7381 cctcgaagcc caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg
     7441 tttatggtca ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac
     7501 cgtagctgca tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc
     7561 aatagccgat ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag
     7621 ggcagattct aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt
     7681 cttgtgaaag agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc
     7741 tctgtccttt attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc
     7801 cattctgtct aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca
     7861 ttccttcgtc tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag
     7921 ttgctcctag aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc
     7981 caatgtcatt ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca
     8041 ctgaagttgc taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa
     8101 ccgtcatatt tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta
     8161 atggaaatag atattgattc caacttccca taaagataag taaaccacta gtaataatcg
     8221 taggtgataa aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga
     8281 ttctggcagc ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca
     8341 ctgaagttgt actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta
     8401 aacgactcat aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata
     8461 gcataactgt attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac
     8521 ccgccattgt agaactggct gtagctgaaa ctgtactgat tacagcaata acaatcgaat
     8581 tccaaataat caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca
     8641 tatccccaaa cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag
     8701 ctacaatcat atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt
     8761 acgtaaaaat tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat
     8821 tgaatagccg ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt
     8881 ccaaaatcaa ccatttcaaa acttgtttta tataaattga ctcctacgac caaagctgaa
     8941 ttttctggtc cattggaagt cccaaagaat atatttggga tattgaaatt acccaagcca
     9001 ttgatagtgg ctaaaactgt ggccattaaa atgattggct ttagcatggg gatcgttaat
     9061 aaaaagaatt gccgaaattt cgatgctcca tctatttcag cactttcgta tatctcttta
     9121 gaaatccctt gaagaccagc taaaaataaa atagtgttat atcctacact aagccaaatc
     9181 atcgtgatga tcattgatat tcgtatccaa aaaggatttg ttaaccactg tacaccgtta
     9241 attcctagcg cattcaaaaa tccattgaat aacccagtcg gattgaaaaa agtcatgaat
     9301 acggaagcta cagccaccgt tgatgtaatg gttggaatat aatagacagt tctaaaaaat
     9361 ccttttcctt ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc taatagcatc
     9421 gttattggaa ttgatcctgc catgataatc aaaacattcg tgtaagattt aataaacaat
     9481 ggatctcgga agatagctag ataattgtca aatccaacaa attcaaatga gtagccaatt
     9541 ttcataaaac taaaagcaaa cgagataatg atgggaatca aaatcgtcac aataaagaaa
     9601 ataacaaaag gtgtgaccaa tccataagca ctttttttat gagacgtatc tgttgctttt
     9661 ttcttgctgt tctttattgt cttgcttggt aacgaagatt gcatcatcgt taagtcctcc
     9721 taatattttt taatcaccag ttaattttac gccgtactta tgagcaaaat tttctgcttc
     9781 ttttagatat gaattttcaa aatcacctgt ttgccaatag ttatatgcag cggtagtcaa
     9841 ataagacgta gcatctgaat aagtggctgg taaaacagta tttgatgtat tttcattgat
     9901 tttattcaat agttcatagt atttttgtcc tccttagtag tcaaactttt tctcagctgc
     9961 ttctgtttga taggccaatg agttagatgt agccacgccc agctccagtg cgttggctaa
    10021 gcattcttca tctgtcagca taaatgtaag aaattgttgt gccacttgtg gatttttggc
    10081 atctttaggg acataaaaac ttgatcctcc tgatactgca ccttttcctg gattttcttt
    10141 tgaaaaagga atcagtggag ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga
    10201 catattcgta gcataccagc cgccagcaac aaacattgca gattcttgaa atgctttctc
    10261 agcatcttgt gcaccgtaga aatgtacaat atctgagtcg atcatttctt tgattaactt
    10321 tgcagcatgt ttcgcttctt tagtattcag acggatattt ccttcttcat caagcaaaga
    10381 ttgattctgc tgggctagca tgttgataaa caattcttgg tccccagatt ctggcaaggc
    10441 aattccaaaa ctaccagttt tttcttttaa tgcttttgcc acctggatat attcttccca
    10501 agaactgatt tcttcaaaat ctacacctat ttcagcaaat ttttttggtt gataataaca
    10561 aagtgctggg gtaaaatcac tagcccaagg aataatcgtt tcattactcg tttgttgacg
    10621 caaaatattc aatttttgat cataaaactg atcgccatat ttgtcataaa atccatattc
    10681 atcagcacta taaaaagcat cttgaaattt ttccagcaca cctgctgctg aagtgtctgc
    10741 catggagata atatccggca tatcttcgtg tgttaccaat tttggggtga tcatttgtac
    10801 tgttgctgtt cccgaagcta cattcacaat ttttaagtca atatttgctc cagttttgcg
    10861 gttatagatt tttgcagcat tttcaacata gtttgtcagt ttgccgtttg cgacatatac
    10921 ttctaatgta gtggcttttt ttatcattgg atctgcgatg tttgctgtta cttcttcgtt
    10981 agtttttcca catgcactta agctgaataa tagggtaatg tttagcaaag ctatcaccaa
    11041 tttgtatctt tttttcatgt ctatcccttc tttcttttca agttcttata taaaacgctt
    11101 tcacttatgt atattaacgg aattagtaac acaaatattt atataaaaaa atattttact
    11161 tgtcgttttg gcttttcatt tttgttataa tagtcatt
//
LOCUS       DANKAY010000087        11127 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000087 DANKAY010000000
VERSION     DANKAY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            6..305
                     /locus_tag="PWY98_002199"
     CDS             6..305
                     /locus_tag="PWY98_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037936.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWY98_002200"
     CDS             378..1739
                     /locus_tag="PWY98_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2037937.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWY98_002201"
     CDS             1761..2021
                     /locus_tag="PWY98_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037938.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWY98_002202"
     CDS             2035..2787
                     /locus_tag="PWY98_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2037939.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWY98_002203"
     CDS             complement(2881..3486)
                     /locus_tag="PWY98_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2037940.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWY98_002204"
     CDS             complement(3539..4513)
                     /locus_tag="PWY98_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2037941.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWY98_002205"
     CDS             complement(4891..5610)
                     /locus_tag="PWY98_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2037942.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWY98_002206"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWY98_002206"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2037943.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWY98_002207"
     CDS             7161..8558
                     /locus_tag="PWY98_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2037944.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWY98_002208"
     CDS             8639..9079
                     /locus_tag="PWY98_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2037945.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWY98_002209"
     CDS             9227..9553
                     /locus_tag="PWY98_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2037946.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PWY98_002210"
     CDS             9554..10303
                     /locus_tag="PWY98_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037947.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PWY98_002211"
     CDS             complement(10383..10838)
                     /locus_tag="PWY98_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2037948.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3531 a         1949 c         2290 g         3357 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctgg
//
LOCUS       DANKAY010000088        11058 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000088 DANKAY010000000
VERSION     DANKAY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(601..1449)
                     /locus_tag="PWY98_002212"
     CDS             complement(601..1449)
                     /locus_tag="PWY98_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2037949.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1448..2515
                     /gene="gnd"
                     /locus_tag="PWY98_002213"
     CDS             1448..2515
                     /gene="gnd"
                     /locus_tag="PWY98_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2037950.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2517..3518
                     /locus_tag="PWY98_002214"
     CDS             2517..3518
                     /locus_tag="PWY98_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2037951.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3515..3799
                     /locus_tag="PWY98_002215"
     CDS             3515..3799
                     /locus_tag="PWY98_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2037952.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3832..4629
                     /locus_tag="PWY98_002216"
     CDS             3832..4629
                     /locus_tag="PWY98_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2037953.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4643..5476
                     /locus_tag="PWY98_002217"
     CDS             4643..5476
                     /locus_tag="PWY98_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2037954.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5494..6351
                     /locus_tag="PWY98_002218"
     CDS             5494..6351
                     /locus_tag="PWY98_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2037955.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6373..6882
                     /locus_tag="PWY98_002219"
     CDS             6373..6882
                     /locus_tag="PWY98_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2037956.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6919..7320
                     /locus_tag="PWY98_002220"
     CDS             6919..7320
                     /locus_tag="PWY98_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037957.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7432..7785
                     /locus_tag="PWY98_002221"
     CDS             7432..7785
                     /locus_tag="PWY98_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037958.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7819..8262
                     /locus_tag="PWY98_002222"
     CDS             7819..8262
                     /locus_tag="PWY98_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2037959.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8279..9364
                     /gene="uxuA"
                     /locus_tag="PWY98_002223"
     CDS             8279..9364
                     /gene="uxuA"
                     /locus_tag="PWY98_002223"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2037960.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9357..10070
                     /locus_tag="PWY98_002224"
     CDS             9357..10070
                     /locus_tag="PWY98_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2037961.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(10277..10426)
                     /locus_tag="PWY98_002225"
     CDS             complement(10277..10426)
                     /locus_tag="PWY98_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037962.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10822..11022
                     /locus_tag="PWY98_002226"
     CDS             10822..11022
                     /locus_tag="PWY98_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2037963.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3737 a         1742 c         2363 g         3216 t
ORIGIN      
        1 atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag cgaacttgaa
       61 acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta caaagtttac
      121 catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag atttaggata
      181 cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt ttacataaca
      241 atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc aaaagccagt
      301 gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa aataagaaac
      361 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
      421 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      481 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      541 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      601 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      661 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      721 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      781 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      841 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      901 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      961 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
     1021 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
     1081 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
     1141 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
     1201 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
     1261 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
     1321 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1381 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1441 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1501 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1561 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1621 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1681 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1741 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1801 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1861 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1921 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1981 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     2041 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     2101 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     2161 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     2221 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     2281 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     2341 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2401 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2461 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2521 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2581 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2641 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2701 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2761 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2821 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2881 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2941 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     3001 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     3061 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     3121 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     3181 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     3241 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     3301 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3361 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3421 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3481 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3541 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3601 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3661 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3721 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3781 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3841 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3901 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3961 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     4021 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     4081 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     4141 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     4201 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     4261 gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat gcatgacgct
     4321 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4381 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4441 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4501 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4561 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4621 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4681 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4741 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4801 gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc caggaggatc
     4861 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4921 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4981 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     5041 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     5101 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     5161 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     5221 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     5281 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     5341 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5401 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5461 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5521 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5581 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5641 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5701 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5761 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5821 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5881 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5941 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     6001 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaaag
     6061 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     6121 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     6181 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     6241 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     6301 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6361 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6421 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6481 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6541 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6601 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6661 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6721 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6781 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6841 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6901 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6961 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     7021 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     7081 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     7141 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     7201 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     7261 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     7321 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7381 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7441 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7501 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7561 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7621 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7681 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7741 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7801 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7861 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7921 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7981 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     8041 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     8101 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     8161 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     8221 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     8281 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     8341 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8401 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8461 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8521 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8581 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8641 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8701 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8761 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8821 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8881 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8941 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     9001 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     9061 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     9121 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     9181 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     9241 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     9301 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9361 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9421 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9481 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9541 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9601 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9661 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9721 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9781 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9841 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9901 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9961 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
    10021 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
    10081 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
    10141 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
    10201 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
    10261 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
    10321 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10381 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10441 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10501 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10561 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10621 cgaagacaat agaagacaat gaaatttata tttcattgca ttatccttgt gttattagaa
    10681 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10741 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10801 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10861 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10921 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10981 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    11041 cgtcttttcg aaagaaaa
//
LOCUS       DANKAY010000089        10852 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000089 DANKAY010000000
VERSION     DANKAY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            524..997
                     /locus_tag="PWY98_002227"
     CDS             524..997
                     /locus_tag="PWY98_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2037964.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1006..1233
                     /locus_tag="PWY98_002228"
     CDS             1006..1233
                     /locus_tag="PWY98_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2037965.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1869..2240
                     /locus_tag="PWY98_002229"
     CDS             1869..2240
                     /locus_tag="PWY98_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037966.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2496..2738)
                     /locus_tag="PWY98_002230"
     CDS             complement(2496..2738)
                     /locus_tag="PWY98_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2037967.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2770..3672)
                     /locus_tag="PWY98_002231"
     CDS             complement(2770..3672)
                     /locus_tag="PWY98_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2037968.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3685..3873)
                     /locus_tag="PWY98_002232"
     CDS             complement(3685..3873)
                     /locus_tag="PWY98_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2037969.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3887..4450)
                     /gene="amaP"
                     /locus_tag="PWY98_002233"
     CDS             complement(3887..4450)
                     /gene="amaP"
                     /locus_tag="PWY98_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2037970.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4488..5381)
                     /locus_tag="PWY98_002234"
     CDS             complement(4488..5381)
                     /locus_tag="PWY98_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2037971.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5459..6382)
                     /locus_tag="PWY98_002235"
     CDS             complement(5459..6382)
                     /locus_tag="PWY98_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2037972.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6431..6781)
                     /locus_tag="PWY98_002236"
     CDS             complement(6431..6781)
                     /locus_tag="PWY98_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2037973.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6814..7716)
                     /locus_tag="PWY98_002237"
     CDS             complement(6814..7716)
                     /locus_tag="PWY98_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2037974.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7709..8566)
                     /locus_tag="PWY98_002238"
     CDS             complement(7709..8566)
                     /locus_tag="PWY98_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2037975.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8900..9709
                     /locus_tag="PWY98_002239"
     CDS             8900..9709
                     /locus_tag="PWY98_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2037976.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9749..10246
                     /locus_tag="PWY98_002240"
     CDS             9749..10246
                     /locus_tag="PWY98_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2037977.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10631..10704)
                     /locus_tag="PWY98_002241"
     tRNA            complement(10631..10704)
                     /locus_tag="PWY98_002241"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10668..10670),aa:Ile,seq:cat)
BASE COUNT         3175 a         2045 c         1831 g         3801 t
ORIGIN      
        1 aatccaatgc atctacttgc gcttattaag ctatttcagc tagatagtac caccccatgc
       61 gtcaatgctt gattttcctt ttgtataaca ttgtataagc aataaaggac aaataagtgg
      121 tgattacacc aaaccattga ataagaaaaa ttctgatacg taaataaaca tgaacttgaa
      181 aaaattaatg aatcggttag ctactacgta gttgtctttt ttctcataca gttctgttaa
      241 cttacacttc tcttcgctag ataataaaaa atcctccaac tgtattgtcc aatataatcc
      301 caggcatcta aactagcatt atagaagaga cacacataga atctaaaata actggtttta
      361 aatgtttctt acaatcaaaa aaagctgtta aatgcactcc acaccttcat actctatcat
      421 tttttataaa cgatatattt ttattgtttt tcgataaaaa atagttgttt tacaataatt
      481 attctgctat attttagcta tcaaagataa aagagggata aaaatgaaat ttaaaacatt
      541 gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg gcgtactttt
      601 ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga ccacattatt
      661 tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt tcttattgaa
      721 actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa acatactaaa
      781 aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac ttcctaaatt
      841 tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt
      901 attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc ttgaacatgc
      961 gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc aattatcgtt
     1021 aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg
     1081 ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc catccgtttt
     1141 tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt gttgacctat
     1201 aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac agtaaataaa
     1261 aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct attattcaga
     1321 ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg actcatacaa
     1381 agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt ttcctgttac
     1441 gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac tgatagcagt
     1501 ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac cttgaagtgc
     1561 ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt ttagtaagct
     1621 cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa catttactat
     1681 gtagttttga taaaatgatt tgaataacag tgatggacaa ttctttttta tttttaacat
     1741 tattttgata ataatgatta tttatttaaa aatacattag ataacatttg ttacgacacg
     1801 aaaaagcttc acaaacaaat atcgttattt taaaatgagg gcgaactcac acaattagga
     1861 ggaatagtat gccatttatt gtttactttt ttctttcatt actcgtgatc tatattccat
     1921 taccaacatt tttcctgctc gtcaatcgtc tagcagagca acatgatacg attgctgctc
     1981 ttacgaaact ttgtttactt ctatttgttt gtctagatta ctttacgagc tattattggc
     2041 taaaacagac aagagtaaaa aagagatatt atttcttcct tgtctttttc aatctctttg
     2101 agtttctgat ccattgcttt ttacttcatc agtatcatac gatccatttg tggatcatct
     2161 gggttttcaa agtggtcgta ttattttgta tgttcacctt tcctatctca agaaaattta
     2221 gggaggcatt gttttattaa agaagcttct ctgtatcaat aaagacacat ttaaggaaaa
     2281 acttttaaaa atctaattgg gaatttgccc gcataaataa gaggctgtga caaaagtctt
     2341 gtcacagcct cttattccgt ataaacagtg ttcaaaagct tgcatcttcg ataatatgcc
     2401 caaccaaatg aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgaag
     2461 aacagctttt tcacgacctc tttttaaaag tctttttaca gttttcgagc aaagaatgat
     2521 acgattagta ctaaaatgat ggcaccaatc aatgatggca cgattgccat tcctgcaata
     2581 cttggtcccc aagaacctaa taatgcttga ccaagtgctg atccaactaa acctgctacg
     2641 acatttgcaa taatccccat cgaagaacct ttattcgtga ttgctcctgc aattgctccg
     2701 ataattcctc cgacgataag tgaccaaata aatcccatgt ttcgttcctc ctaatgtttt
     2761 acttccttat tactgaaccc gtggttctgt attttctttt actttttgtg cgccgcttcc
     2821 agctgctgat tttactttat ctgtttgatt cgaagcaaat tcaccggttt ttttcgctgc
     2881 gtcagttact ttgtcttgca ccgtttcttg atctttttca aattcttctt ttgttttgat
     2941 gtccactacg ttagcgttga cttcgatcac ttctaaatgt gtcatatttt ttacttcttt
     3001 gccgattact tcttgcattt cttcaaagat tttttcgata tctttgccat attccacaat
     3061 gacatccata tcaactgcta cttgtttttt accgacttct gtttcgatcc cagccgttac
     3121 attatctgta ttcacaagtt ttcctgccac attagagaag aaaccaccgt ttgcagataa
     3181 taaaccgtct acttgttcaa ttgcgattcc gatgattttt tgcacaactt tatcttcata
     3241 attcagctca ccgcgcattg aatcttcctg ttcagaatca tgctttcctt ggttatttgt
     3301 atgatcagaa ccaagttttc cttgtccgtt tgtgtggtca gaactaagtt tttcttgtgc
     3361 tttcgcatgg tcagaaggat gtttttgctc ttccacaggt ttagaaggat cgattgcatc
     3421 atgttgttct ggaactttag attctggagt tcctggatga tgaggtgcat gcttttctgc
     3481 atcatgtttc ttctcttcta ctccttgttg aacgttaggg actactggat ctggatgtgc
     3541 agatactggt gctgttttgt tcttttcatc gttcacacca tttgattggt ttggtgtcgt
     3601 tgggtctgga tgtgcagaca ctggtttttg gttgttcatt ttattgttat tatttttgtt
     3661 attgttattc atggatgttt cctcctaaga tttaaaatag tgatctaaga ttttcgtttg
     3721 tttcagataa aaaccgatag cgacgccgat cgctgccatg atcaagatca ataaggtctt
     3781 gaaaaagccc acggtgatcc ataaaacggc taatatcaat ccaagtaccc caaagatgat
     3841 agggagcttg tatgtcgaaa atagttcttt catttttggt ccccccctat tctactcgtt
     3901 tgcggtcatt gcttcgttta gctggttctt caaagcctgt gtatttcacc gatactttca
     3961 ctggctcttt tgttcccaat atgtgttgta cttcattttc gatttctttc atcaatgttc
     4021 ctgttttacc aatgagtgct gaagttcgtt tcaactcccc ttttacattg acggcgatct
     4081 tgtttttcgt tgctttgaca tttacttttg gtgaatggat gaattcatct tcatgcaagt
     4141 gagaacgaac cattccttca attgcttttt tatccaacgt caactcccca tcaccatgtt
     4201 tcagtagaaa gtttccgatt gtttttggat aaaacaggaa aacaagtaac gtgataattg
     4261 tcaaaatagc aaatgcaacg gcaagccaaa atacatattg ttgcacgtat gaattagtga
     4321 tggaaaagaa tcgaaacttc tctagctgaa aaggcaatgg ataggttgca cttaatagta
     4381 tcgcaaacaa aagcacagat agcaaaagta atgttccgat cactccgatc gtttttacac
     4441 ctttattcat tttcgcacct cctcataggg gtgaaacgat caacttctta taagttgatc
     4501 gcttctccac cagttactcc gtaaatttgg gctgtaacat aacttgcttt attcgatgct
     4561 aagaaaacat aaactggtgc taattctcct ggctgacctg cacgttctag taaactttgc
     4621 tgaccaaatt cagggatcga atcagaaggt tgtccttcgt ctagttgtaa tggtgtccag
     4681 attggaccag gtgctacacc atttacacgg attcctttag gtgcaagctg ttttgctaaa
     4741 ccgatcgtaa agttagcgat tgaagctttt gttgctgcgt aatctaataa atgttcgctt
     4801 ggattgaatg cttgaacaga agttgttgtt acgattgctc ctcctgctgg gatatgcgga
     4861 acagcttctt tcactaaagc aaacatagcg atgatgttta tcataaatgt gtctttgact
     4921 tgctcaagtg gtaactcagc aatagactga caagaaattt gctgtcctgc gtttagtacc
     4981 atcgtatcta gcccgccaag ttcactcacg gctttatcta tgatttcttt aggtgcttgt
     5041 tcgtcacgaa gatcaaaagg tagtaagaca atcttacgtc ctgcaacttt cacgtatttt
     5101 gctacttcct ctgcatcttt ttcttctcct gggaagaatt gaagagcgat atctgcccct
     5161 tctctagcaa atgcaatcgc tgctgcacga ccgatccctg aatctccacc agtgattagt
     5221 acacggcggt tttctagttg attattgcct tcataggatt cttctccaca atcaggaaca
     5281 ggaatcatat tctgctgtaa agctggtgta tcctgatctt gtttaggaaa tttctccgta
     5341 tggtacaagt ttcgcgggtc gattaatttt ggttctgtca ttgctgtatt cctcctagtt
     5401 tttaaattgt attgccaaaa caaatttctt tgtttgatac ctgttttaaa tagattcttt
     5461 aatttgtata atctttccgg tataaatggt aacctaaaac tactgctgct atcaaagagc
     5521 caaagactac gagtataaag cctagagcag aacttttacc cggtaatcct cctgtattca
     5581 ttccccaaat ccctgcgaac aacgccggaa cggaaatgat taatgcagct gaatctagat
     5641 atttcatgat atgatttaga ttattgtcca tcatcccgtt gaatagatca ccaatactct
     5701 ctaataaatc tcgatagatc tcgatcattt tttctgtttg tcgctgtctc aaatggacat
     5761 catagagtag ctgttcatcg tctaaacgtt tgatgaaagt ttcagaattc cataattgat
     5821 ccattgtttc ccgttgatct cttaaggtat gatcgatata tacgatgtct ctttcaagat
     5881 ctgcctgttt atacagttct tcattttccg ttgttttacg agctgcttta tccaaagcgt
     5941 caattcgctt tttcatctct cttagttctt tcacgtaatg ggtgtaaata tctaataatg
     6001 tatacgtgat gatcttttca aacgagttta aatgatttcc atgctttgtc agcagccgat
     6061 ccacaaattc ggtttttcta tcgatacaag taatcaagag attatcggaa agtacaaaag
     6121 atattggcgt taattttttt tcgatacgat tatctgactg atctagatta atcactacca
     6181 aagaaaatgg atgctctagt ttcgtctcat aaaggcgctc catccgagaa acttcttccg
     6241 gatattcggt tcctacgaaa atatcatcag gaagctcgaa ttcttcgatt gcttgctcaa
     6301 tctcttcggg ctttgcacta tcaagtacga gccaattata ttttttttca tctgtttcta
     6361 ttacattctt ttcttcatcc acaaagtagt atctcaatgt atacctcctt tttggacttt
     6421 ctcgaaacta ttagctgaag aagcagttct ttaatttgat attataatcg agtactgcga
     6481 tttcattttc taatgaaaag ctgtcaagct gtttgttgaa gtccatgttc aatcgtttac
     6541 cgaaataatt ttgctctaat ttagatgtat agatgtccaa taatggatgc tcgattttag
     6601 tcgcatattc tccaaattcc gaaacaaaag gaatgatttg aaaaaagctg ccttttgcaa
     6661 aagcaccttt gtcccttaga aatggatcgg aatcattgat aagcgcgagt gcgatttttg
     6721 tattttcccc aaccttttca attaacgtct ctactgcatt tttagacatt gtaatttcca
     6781 ttcctactcc ccctcttcag ttgttgtctt ctattattta acaactacat ctgtttttgg
     6841 ttgaactttt gcatattttg gatatttagg gaatttagct acacaacgga aagccgtcat
     6901 ccctgcatat ttttcgtaat atttcttcgt tggcccttca aaatccttgc tccacggttt
     6961 cacatgtcct gaaaaatgga taatgtaagg attgttgcga gtttcttcgt attcacgttc
     7021 acctgctgct gttggatgtt tttttgcttt tgccatgata tagccttgtg cattccaacg
     7081 cggatgaagt ggtaaccaac ggtcatgtaa gatagcattt aatgcatctt ggtcatggaa
     7141 tcgtaatttt tctggattat gttcgatgaa atctaatact ttttgcgtaa tattttcatc
     7201 taaccatttt ttcacattga tcagcatcaa acctgagttg aaataacgca ttgatttagc
     7261 tgaaattttc attttttcca gtcgttgatg gaacccagcg tcttcaacag ctgctacgat
     7321 tgaatcaccg aagtcaagac gccataattt agagatatct tgtaaagcga tcatgtcgca
     7381 atccatatat aagatacgtt caacttctgt gccgcggaac aattccggaa tcgcaatacg
     7441 ataataagct gtttctggaa tacgatcgct gattaccaca tttgtaaaga attctttatt
     7501 gattttcaag aattccacat ctgagttcat tcgtgcgttt ttcactgaga agcgtaaaag
     7561 ctgtttactt tcaaaatcga tatcatcgtc aatcacatag aaccgaacaa atttagacgg
     7621 gttgcaatta tctaatactg agacaaataa tgcagctaaa tgtggtacga attttgtgtt
     7681 acaacttgcg actactgcta tttctttttt attcattgat ctcgcctact ttcgttagag
     7741 tactgtgtgc taattttttt aagtataaat tggtatacgg atgatctttc aacgtattcc
     7801 aaggtttatc atgtcctgta aagtgaacga tcgaaggttt ttcatttcct tctttatact
     7861 gacgttcata tttttcattt ggtgctggat gacgttcaaa aatcagcgaa gtctgcatgt
     7921 tccattttgg atgaagttgt tcccaatctt cataaagtac agcattcaaa gcatcttggt
     7981 cgtgatagat gattcgatcg ccattttcac tgaggtaatg gatagttttt tctgtaattt
     8041 cttttttatt ccattgatca atgtcaatca ccatcactcc tgaattgaag tacaagtcat
     8101 cggaatcgat tcctaatctt tccaaagctt tcacttggcc tggatcgata atagcgccga
     8161 ttgtttttcc atttaacggc tcatcatata gttttacgat atcgtctagt actaacacat
     8221 ctgaatctaa gtaaaggact ttcttataat cctctttagc taataagttt ggtaaagaaa
     8281 ttcttaagta agcggtagtt gtgatatggt cactgactag gaagtcttcg tagatatctt
     8341 tttccactgt caggaattgg atacttgcat tacttgaata tttatttacc gtttcttcca
     8401 atccttcttt actatattct gatagaccat cgtcaatcac ataaaatttt atgcgtcttg
     8461 ttttattgca attttctaat gcagttgcga tcatcacgct caagtaagga gcataatttt
     8521 cgtctgaagc agttaccaca ggaactacgc cgtatctctt ctccatcaaa atcactcctt
     8581 tttctaatct ttttctaacc tgttctacat ctagaatatt cctcatttgg ttttaaagca
     8641 aaatatatgc tttgttttca ttgaatattt taaaattttt ttatcacaaa acgcatcggt
     8701 aagcgctaac tattcgtttt tcatggcttt ttattcaaag gtattgacca aaaactacta
     8761 tatatagcca cctttttctt aagaatgttt tttgaaaaaa ttcataatga caaagcaagt
     8821 tagtttcatt tttttaaatt ctcatttaat cttaaggtaa caggaaaata gatatgtaaa
     8881 aagaaagtag gaagataaaa tgaaactagc agcgattgat ttagacggta ccttgcttga
     8941 tcgaaatgga aagataccag aagaaaatat ccaagcactt cggaaatttg atgaaaatgg
     9001 cggaatcgta accgtcgcta ccggaaggaa cagcatttct gctaaagata ttttttccga
     9061 attaggaatc ggtggctatt tgatttcttc caatggttca ctgattgctg aaatgaagga
     9121 tggaaaaatg gcgaaagttc tcagacgttc aaaaatagat agcgatacac tgagaaaagc
     9181 cttcttctat gctcaagaag cgaaaatatc catcattgct agccgagaaa cccaagatga
     9241 tcagctgaca ttcgatgaat caaatctagt caaagatgac ccttattatg aacatttcaa
     9301 tttacagaac cattcttttg atcaaatttt aaatatgttg gaagatcctt ctcttagcta
     9361 tcttaaatta gcattaacag ataagagtga agaaaaacta cagaagctac aaagagatct
     9421 gcaagcagat ggtatccaat cagtattctc tgatccgcat ttcttagaaa tcacgcctac
     9481 aggtatcact aaagctcatt cacttgtttt tctggcggat taccttggaa tcgaatcgag
     9541 tgacatcatg gcttttggcg atcaagaaaa cgacattgct atgttgaatt acagcgggtt
     9601 gagtattgcg atggggaatg cacagcgaca agtaaaagaa attgcaaacc aagtaacact
     9661 gacgaatgaa gaagctggtg ttgcttatat tttaaaaaac ttctattaaa aagaataaaa
     9721 tgacgatatg aatgtaggag gaaagaaaat gttagttatt attgatatgc aaaatcatat
     9781 tcttgatcca aacagcgaat tttatattcc agagtcagat caattggcag aacgtattgc
     9841 tcaaagatta gctaaagcac gccaaaataa tgaatatatt ctcttcacac gggatatacc
     9901 agttgaactg aaggacaaag aagaagaacg agaagatttg caattgatcc ctcttctttc
     9961 tccacaagaa aatgaacgag tgattaaaaa gtattacttt acgattccac ctgaaacttt
    10021 agctgaaatc caggaaactt tatttgaaag caaagaagaa caaaaagaga tcgaagtagt
    10081 cgggattgaa acaaacttgt gtgtactctc taatctgata ggcctgcaga gtgcttttcc
    10141 tgaagcagac ttttttgttg atccaatgtt agtctccagt agaaaacacg gagaatccgc
    10201 tttagagctg ttgaaagatt tcaacgtctc tatcgtcgaa tcataaaaat atctatcttc
    10261 atttacgata tttaacacac aataaaaaaa gcacgcagaa ctgttttagt cctacgtgct
    10321 ttttctatca aagtagattg gcttttttat gacacagagt gaaacggcag gctcacgacc
    10381 tctttcttaa atggtgttca atcagctgac cctcggtatt agcttaacaa acggcaaaat
    10441 atgaggaact atttttccgt ttgttaagct aatactcagg tcaaaacgct gatttcacaa
    10501 cctcttcctt aaacggtatt caaaagcttg catctgcagt aataagaaca gccaagtaaa
    10561 aacataagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    10621 caacctctta tgtaccttgt aggactcgaa cctacgaccg gacggttatg agccgtctgc
    10681 tctaaccaac tgagctaaag gtacatcata aataaaattt atttttgtta cttttcaact
    10741 atagcacgaa tgacaaactc tcacaagttt caaaatataa gtaaaataat attgttattc
    10801 ttatttcttt tacttgtgtc ataactattt tgttacttta atttttattt gc
//
LOCUS       DANKAY010000090        10818 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000090 DANKAY010000000
VERSION     DANKAY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..1778)
                     /gene="glmS"
                     /locus_tag="PWY98_002242"
     CDS             complement(<1..1778)
                     /gene="glmS"
                     /locus_tag="PWY98_002242"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2037978.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPR"
     gene            2187..2477
                     /locus_tag="PWY98_002243"
     CDS             2187..2477
                     /locus_tag="PWY98_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037979.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2490..3515
                     /gene="sppA"
                     /locus_tag="PWY98_002244"
     CDS             2490..3515
                     /gene="sppA"
                     /locus_tag="PWY98_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2037980.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3518..4177
                     /locus_tag="PWY98_002245"
     CDS             3518..4177
                     /locus_tag="PWY98_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2037981.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4334..5266
                     /locus_tag="PWY98_002246"
     CDS             4334..5266
                     /locus_tag="PWY98_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037982.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5420..6709
                     /locus_tag="PWY98_002247"
     CDS             5420..6709
                     /locus_tag="PWY98_002247"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2037983.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6956..8206
                     /gene="clpX"
                     /locus_tag="PWY98_002248"
     CDS             6956..8206
                     /gene="clpX"
                     /locus_tag="PWY98_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2037984.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8289..8876
                     /locus_tag="PWY98_002249"
     CDS             8289..8876
                     /locus_tag="PWY98_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2037985.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8950..9105)
                     /locus_tag="PWY98_002250"
     CDS             complement(8950..9105)
                     /locus_tag="PWY98_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037986.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            9241..10569
                     /locus_tag="PWY98_002251"
     CDS             9241..10569
                     /locus_tag="PWY98_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037987.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            10580..10789
                     /locus_tag="PWY98_002252"
     CDS             10580..10789
                     /locus_tag="PWY98_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037988.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         3603 a         1867 c         2240 g         3108 t
ORIGIN      
        1 ttacgtggtt tgtcaacgtc gaatccccgt aatagcgttg cgtaatatgc aagtaattga
       61 gtcggtacga ctgtggttag agtagttaga taagggtgga cagttggtag caccagctgg
      121 tcactttcct tttctaatcc ctctgagaca ataactagtg ttttagcgcc acggctttcg
      181 acttctttca ggtttcctcg agtatgcgca ccagtcactt cttcactgat gattccgatc
      241 actggtgttc cttcttcgat caaagcaatc gttccatgtt tcaattcgcc agcggcaaaa
      301 ccttctgcct ggatataact gatttctttc aatttcaaag cagcttcaag agaaacgttg
      361 tagtcagcac cacgtccaat gtaaaaggca tttcttgtat cgctgagata tacttctgcc
      421 aactcttcta aataatcttt ttgggcgatg accgattcca tcgcattagc aactaaactc
      481 aattcatgag tgatatcgaa tacaagagaa gtttcaaatt cttttttatc accgatagct
      541 ttggctaata aagttagtac agctatttgt gcagtataag ctttcgttga tgctactgcg
      601 atttcgggtc cagcatgcaa tagcagtgta aaggatgctt cccgcgaaag agtagaacca
      661 gcaacatttg taatcgtaag tgaagggtag ccgagacggt tgatttttac cagcacttgg
      721 cggctgtctg ctgtttcgcc actttgactc aagaaaataa agaatggttt ttttgaaaga
      781 agaggtgtgt tatagccaaa ttcactggct aaatgaacct caacaggaat ctgtgtcaat
      841 ttttccaaag tctgtttacc tgctaacccc gcatggtaac tagttccgca agcaacgata
      901 tagattcgat cgctatcaga caatgtatcg accagctttt gatcaagaga gacacgattg
      961 tcttctgtta cgtatttttg agcgatacga cgcatgataa taggctgttc gtcgatttct
     1021 ttcagcatat aaaaaggata ggtacctttc tcgatatctg aagcatctac ttgtgcttcg
     1081 aatgaagaac gttctacgat gtcacctgca aaattttgaa taataacttt ttctgatgtg
     1141 acggtaacca tctcttcatc ttcaatctca acgaattcct tcgtatgagc aagcatagcc
     1201 attgcatcgc tggcaatcac attgaatcca tcgccgagtc cgattaatag tggactttta
     1261 tttttagcga cgtaaataac atcaggtgct gtccgatcaa tcaaagcgaa agcgtaagaa
     1321 cctttgatga cccgcaaagc ttttaaaaac gcctcttgcg ttgtagtatc cttcgcgaaa
     1381 gtttcaatta gatgagcgat aatctcagta tcggtctcac caataaagtg gtcatttgct
     1441 aaataagcaa ttttaagttc ttcaaagttt tcgattacgc cgttgtgaac gagcacgaag
     1501 cgtccacttt gggaagtatg tggatgggcg ttttcttcag aaggttgccc gtgagttgcc
     1561 catcgtgtat gaccaatacc tatatttcct gtagtatcaa tatttatttt gtttttcaaa
     1621 ttttgaattc tgccttgtgc tttgaccaga taatcagttg tgccatctga gacaaagata
     1681 ccagctgaat cataaccacg gtattctaat ttttctagac cgtcaatcaa tcctggtgtt
     1741 acatttttta atccaatcat tccaacaatt ccgcacataa aaaacatcct actttctata
     1801 atgaattcgt atgaatgctt ccagctgcag gaaaatcctt ttgttttatc tttgcagaca
     1861 ttttttgtgg attttctgta gagtgcagtc cctagggcca tccgccgaat ctttcgataa
     1921 accctctcct cgtcatctgt ttacacagac ctggcgctat cctaaaaggt ataactatcc
     1981 tcctttttgt tttttatctg gaaacattta aaatcataac cttttgcgaa tgttcttgtc
     2041 aacaaaaaaa gaataaaaaa taaattggta tatatcaaat tctttgtcaa atgtcctttt
     2101 tatgtcaaaa aataatgggt ttttatctat catctttttc aggagttcgg tacaatagaa
     2161 agtaatcgga tagaggagga gtaaaaatgg attcaaaaat tgagatcatg acactgggaa
     2221 tgttaaaaaa acaattgtcc aaattcgaag catcagctgg tgtatcagat gatacgaaaa
     2281 tatttttaga tactggctgg gatagcatcc aagaaatcgc acctgatgcc ttggaagttg
     2341 ctcaagctcg tgagtttacg gtagaagatg aattaacaaa agagtcgttt tccgggtatg
     2401 cgcgggaaga aaaagcagag cggttcgaca cttctgagca gtcagaaact gtgatcgtca
     2461 taaaaaactt gtattagaaa ggagaaagaa tgaataaaag acgttgggca gcagttctga
     2521 ttgctgcagg attatttgtg gtttcgttag tttcagctag tttgactaag cctcaggaaa
     2581 acgatcaaat tgagggattg aacagttggt tatatgggga tgaagaacta gctccaaatg
     2641 tgttggaaga gggagacagt acgcaaaaaa tcgtaaaatt atcagtagat ggaacgattg
     2701 ctgcgggagg gtcttcaggt ttatttgctt ctgaaggtta caatcataaa aattttatgg
     2761 aacaactgaa ggcgattgaa gaagatccaa cagtcaaagg tatcttttta gaagtaaatt
     2821 ctcctggtgg cggtgtctac gaaagtgcgg aaatcgctaa gaaattggat acgattcgta
     2881 aagaacatga tatcccaatg tatgtcagca tgaaaaacat ggcagctagc ggtggctact
     2941 atatttctgc tcaagcagat aaaatctttg caacagaaga aacagtgacc ggctcaattg
     3001 gtgtgatcat gtcaggattg aattattcag gacttctaga aaaactgggt gtggaagata
     3061 caacagttaa aagcggagcg ttgaaagata tgggttcagg aacgcgacca gagacaaaag
     3121 aagaagaagc ggtgcttcaa gcatatatcg acagcgcata tagtcgtttt gtagcaatcg
     3181 tcagcgaggg acggaataaa tctgaagaag aagtcaaaaa aattgctgat ggaaggattt
     3241 atgatggtgt ccaagcaaaa gaagtaggtt tagtcgatga attgggattt cctgaagatg
     3301 ctttaaaagc tatacgaaag gaacaacagc ttgaagatgc ggaactagta gaatattctt
     3361 ccacgccatc tgggtttgct agtacatggt taggaagtaa actagcagaa ttccagggat
     3421 tgaagccttc tgagactagt cagatcttat ccgttcttga gaatctaggg acagcggaat
     3481 caccaaaagc aatgtattat tacggaggtg agtaagcatg tcagagcttt caaatgaaaa
     3541 taaggaacaa gaaaaagata agcaacagca gcctgctgta ggacctgact ccaacttttc
     3601 ccaaaaggta ctgagctctt ttgaaagaga gcctttgacg cccgaagaaa tcaaaaagaa
     3661 acaacgtaaa tggaaaaagt atacggacac aagaaatgaa ccgccaaaag caaatgattt
     3721 tcctaagtat ttctatgcag gtttttttat ccgaatgttt gccttcttgg tcgatctttt
     3781 gtgtatcgga gctattacgc gtttaacact tggtattgcc aaaaatctag gttggcttaa
     3841 tttttcagat tcttatctga gtctctacgg attcacagcc ttacttattt atctagcgta
     3901 ttttatttta ttgacgaaat tcaatcatgg ccaaacgatt gggaaaatga tctttggtat
     3961 tcgagtcatc agctttgatg aagaagaact tagctggtca accgttttag ttagagaagg
     4021 tgcttgccgg ttcattctaa aatttccttt gctgatgtta gggtatcttc cagcagtctt
     4081 taatcagaaa aagcaacatc tgggcgattt tttcactaat acaagtgttg ttacgatcaa
     4141 tctgatcaaa gcgttcaacc aggaagtcaa cagctgatga gcaataaaaa gcagtaacag
     4201 ttgaaagcac cttctaaaag tcagatctaa aatctagctt ttagagggtg cttcagttat
     4261 ttttaattgt catgcttttt ttgttttgct actattgaac atgagctgaa tatgagcaag
     4321 aggaggacac tgcgtgaagc cgattgattt tccagaaaaa tatgagaatt taatgcgagt
     4381 ggcacaacaa gcactagcaa accagcaata tcagcaggca aaagaattat ttcaacgagc
     4441 ttacgagttg aaagaaagct ttgaggctaa tagcctgctt gttttttgtc tttacgagtt
     4501 ggacgagaaa aaagaagcat tgaagcaagc actcttgcat gaaaaacagt atcttcaaaa
     4561 tgaggagttt gctgaatttt attttgactt gttaatcagt acacaagatt ttttatatgc
     4621 tagaaaattg atagcaagta cagattttta tgaagctttc gagcagcggg tcattgaaaa
     4681 aatccaattt gcagaagaat tatccggaca aatggagagg cagaagatca aggcactcca
     4741 caataaatcg gaggaacttc cttcgttgga acctgcacgt cagcttgcgt ctattgatga
     4801 gatcgaacag cttccttatc atgagtttat ccaaacagcg agtaaactta ttgtatcgcc
     4861 agaggtccat attttagcaa gggcaaaatt actggagaca cttaggcaat tgaacgaatg
     4921 ccatcaagtt ttctatttga cgattgaaga aaagttagtc aaagtcatac caaaagatct
     4981 gccaaagccg cagcagcagg caagttacca gcaattatgt gtattttcag atcgttacgg
     5041 gaatgaagat gcgctgctta gttccgtgtt aaaagaagaa ttcacgttgc aatctgcaat
     5101 tgtttatcct gtctatgata catacatcga agacccgaag cgctggtttc aactgactgt
     5161 agaggcgtgt acaggaaaaa cgatgtatga aggtacagaa gatgaaaaac aagatttttt
     5221 aaaaaaaaga gaaaaaattt tgcagcaaat gatcttcttc cattagattt tatcagtgaa
     5281 aaagataaaa atgttatgaa aaacaaggat tctatccatt caatatgttt acaatttgaa
     5341 agggttagtg tactatttaa aggtatgcat gaaacgtttt tgatgtataa aatgaatttc
     5401 taaactcgga gggaattaaa tgactgcaac atttgaaaaa aaaggcacga atgatggtgt
     5461 tttaaaattt acgattgcgc aagacgaaat ccaaaaagga ttaacaactg cgtttaataa
     5521 agtgaaaaaa agcttgaacg tacctggatt ccgtaaaggg aaagtttctc gtcaagtatt
     5581 caaccgtatg tacggtgaag aagctttata tgaagatgca ttgaatgctg tattaccaga
     5641 agcttatgaa gctgctgtca aagaagctgg catcgatcct gtttctcaac caaaaatcga
     5701 tgttgaaagc atggaaaaag gtcaagactg ggtaatcact gctgaagtaa cagtgaaacc
     5761 agaagtaaaa ttaggacaat ataaagatct agttgttgaa aaacaagacc gtgaagtaac
     5821 agatgctgac gtggatgcac gtatcaaacg tgaacaagaa gcacaagctg aattagttat
     5881 caaagaagat gctgcagctg aaaatggcga tacagtagta atcgacttcg aaggctttgt
     5941 agatggcgaa gcgtttgatg gcggaaaagg cgaaaattac tcacttgaat taggttcagg
     6001 ctcattcatt ccaggttttg aagatcaatt agttggtcat aaatcaggtg aaagtctaga
     6061 tgtaaatgta actttcccag aagattatca agcagaagat cttgctggaa aagaagcagt
     6121 cttcaaagtg acaatccatg aagtaaaagc aaaagaagtg cctgaattag atgacgaatt
     6181 cgcaaaagat gttgatgacg aagtatcaac attagacgaa ttgaaagaaa aatatcgcaa
     6241 agaattagta gaaacaaaag aaaaagcagc ggacgatgca aaagatgaag cagcgatccg
     6301 tatggctgtt gaaaacgcag aaatcgttga attgccacac gtgatggttc atgatgaagt
     6361 acatcgttca atggacgaat tcttgaacaa catgcaacgt caaggtatct ctcctgaaat
     6421 gtactatcaa ttaactggtt ctacagaaga agacttgcat aaacaattcg aaggtgaagc
     6481 agaaatgcgc acaagaacaa atcttgtgat cgaagcaatt gcagctgctg aaaatcttca
     6541 agcaacagaa gaagaaatca atgcagaaat ccaagaatta tcagaaacat acaacatgcc
     6601 aatcgaacaa atcaaacgcg tgttgacaga agatatgctg gaacatgatg tgacaatgaa
     6661 aaaagcaatc gaattgatca caggtacagc gacagaagcg aaagaataga aatcaagtaa
     6721 taaatcattt ctgaagaagc tacttctaga aaattgttta ttcgaaataa gggactgtga
     6781 cacgattttg tcacagttcc ttattttttg ttaagagtaa aaaatgcaaa ggtataggtc
     6841 agttgatcct ttgttaaaca ctttttcttt ttactaaact atgttatgat agtaaaagac
     6901 tgtaaagtct ttgatagttt cttgcatact ttttgcaagt taaggggtga agaccatgta
     6961 tgacaatcca agtagcaatg agaccgtacg ctgttctttt tgcgggaaaa cccaagaaga
     7021 agtgaaaaag atcgtagctg gtccaggggt ctacatctgt aatgaatgta tcgacctatg
     7081 taaagaaatc attgatgaag aattttatga agaagctgta cgcgaattca ctgaagtccc
     7141 taaaccacta gaaattcttg aagtattgaa taactacgtg attggacaag atcgcgcaaa
     7201 acgctcgtta gctgttgccg tatacaatca ctataaacgt gtcaatcagc aagcacaaga
     7261 aaacacagat gatgttgaac tgcaaaaaag caatatctgc ttgatcggac ctacagggtc
     7321 tggtaaaaca tttttagcac aaacattagc aaaaacattg aatgtacctt ttgcaatcgc
     7381 agatgcgaca agtttgacag aagctggtta tgtgggggaa gatgtagaaa acattttgct
     7441 taaattgctt caatctgctg attacaatgt ggaacttgca gaaaaaggga tcatctatat
     7501 tgatgagatc gataaaattg cgcgtaaaag cgaaaatgta tctattactc gtgacgtttc
     7561 cggcgaaggt gtccaacaag ccttgctgaa aattctagaa ggaactgttg caagtgtgcc
     7621 acctcagggc ggacgtaaac atccacatca agaatttatt cagattgata cgacgaacat
     7681 attgttcatc gtaggtggtg catttgatgg catcgaaacg attgtgaaga atcgtatggg
     7741 cgaaaagacg attggatttg gtacacaaaa ccaaaaactt tctgaagacg aaagtgtcat
     7801 gcagcatatc attcctgaag atttactaaa attcggattg atccctgagt ttatcggacg
     7861 attacctgtt atggcagctt tagagaaatt gacaacagat gatctggtac ggatcttgac
     7921 tgagccgaaa aatgcgctag tcaaacaata tcaaaaactt ctttctctag ataataccga
     7981 acttgaattc gaacctgaag cgttgagagc cattgcgaaa aaagcaatcg aacgcaatac
     8041 cggtgctcga ggacttcgtt ccatcattga agaaatcatg atggatgtga tgtttgatat
     8101 cccttcaaat gaaaacatcg aaaaagtgat tatcacaaaa gaagcagctg aattgtctgg
     8161 tgagccaaca gtaatctata aagattcaga gaaaaaagcg ggttgatgaa aagaggctgc
     8221 cgtaaaagtc attttggctt ttgcgacagc cgcttgcttt attcatcacc ttgttaagga
     8281 gagagaccat gaaagtccat caagctgaaa ttatcataag tgcagtggca ccaaaacaat
     8341 atccagatac acaattgcca gaaatagctc tggcaggacg atcaaatgta gggaaatctt
     8401 cttttatcaa cacattgatt gatcgaaaga atttagcccg tacttccggt aagccaggaa
     8461 agacccagac attgaatttt tatttgatcg agaacgcatt acattttgtc gatgttccgg
     8521 gatatgggta tgcaaaagta tccaaaactg agcgagcaaa atggggaaag atgatcgaga
     8581 catatttaac gactagagac cagctacgtg cggtcgtttc tttagtagat ttgcgtcata
     8641 agccttctca agaagatatc cagatgtatc aatttttgaa gtattatgat atccctgtaa
     8701 ttgttgttgc cactaaggca gataagatcc ctcgtggtaa atggaacaag catgagagcg
     8761 aaatcaaaaa agcgttggat tttgatcgca atgacacgtt tattctcttt tcttccgtta
     8821 caaaacaggg aaaagaagaa gcgtgggcag caatcgaaca agcaattgaa tcataaaaag
     8881 aataaaaaag acttaccagt caaactcttt cataaaagtt tggctggtaa gtcttttttt
     8941 gagcttaact tacttttgtt tcttgtcttt tttcttttca ttttgtggct gtggttttag
     9001 aaatcgatcg atctcttctt ttggtgcaac tgttttttcc ggatcgacag caaatgattc
     9061 gaaaagttta tctaaattat ctgtacgttc aaatttcaac cccattgtaa acacctcttt
     9121 gtttcttttt acttagtcta tcatgtaatc gtccaaattg gtttctaaga cataaagttt
     9181 tagtttttct ttatcattgg ttataattaa attataaaaa gatttgaaag aggtgagaaa
     9241 atgattggcg aagataaatt attgatgact atccagctca cagacttaaa tgaagaagaa
     9301 aaaaaacagg aaataagcga ttgggctatt ttgacacgtc tgttgatcca gccaacattt
     9361 atccgctctt tcacacaaca agcggatccg tatgatcttc gaaagcttca agaaaaaata
     9421 atgctagcat cggtaagaga cgaaagttgg ttagttgtag gaaatgacga gaacgaatgt
     9481 agttttcgct tggaagataa tcaattattg ataaaaaatg tattgtctat ctctgttttc
     9541 aaagaaaagc aatttttgat tcgagacttc attcagaaaa aaatgattgc tcatggggtg
     9601 ttcgcctata tgcgagctta tagcgagttt atctatcaca atacaaaaca gatctctcaa
     9661 cggctattgt ttgagaaaaa ggatgaaatc gaacatttac ctaaaatgaa acaacaaaac
     9721 ggggaagtag tagtcgattg caaccaattt ccaggatacg atctttttta ccaaggttta
     9781 tgtttcactt cgtgttggga aatgtattat agtcgctatt atcatcaaat catccctaaa
     9841 ccgatttttc tcgacatcca acaagtagaa aaagtgaagg aattagaaaa cgaagtcata
     9901 tgtatccagc tttatcgaga cccctttaac tggcaaaagc caaacaatca aatgtttcaa
     9961 tcctatttta gggaccagct tgggtttgat catcttgctt gggacaatgg tgtcggttta
    10021 ttgaaaccgc cattcgttga atacatttac actgaccata cgatccaatc tgtccagtat
    10081 cagaatgcac aaatgcaacc tgttcctaaa aagaatgctt cattttttgt aacaaaaagt
    10141 tatgatatcc aacaaggaga ttataaagaa aggcgtgttc gcggaacact aaatgctcag
    10201 gcgtattttc cttgggttga cgagaatcga tcacgcatga tgtgctataa agttatcgat
    10261 ccgactgttg ctttggataa tggaatcgaa gcttattgtt attatattag ggaatatcta
    10321 gaagtagagg taacagatga aaaatatcaa gagtatcttt tatccttaag aatctatgtt
    10381 ccttcagaat ccctgtcaga attaccgtta aaggaaatca aacatagact ctctgatgtt
    10441 accttcaaac gaataaagaa aaagcggcga agtatccaat tcgatgtcaa gaaaggtgag
    10501 cagcatctgc gtgtgcaatt tttagattac cgagaattag aacaattgat tactttacaa
    10561 aaaatatgag gtgaagaaaa tggataaaca actctattat cgaataatgg ttccggcatc
    10621 attattatcc gtctgtctct tttttgttcc taattttcct attcgatcag tcatcggttt
    10681 tttggtcatg tttggcggga tgggacttta ttattatctt cttcataagg aaagaaaacc
    10741 tgcttctgtt tatatgaaaa aaacaagaag cgaaaataaa aaacgataaa acggcttgat
    10801 gatagacaaa aaaataaa
//
LOCUS       DANKAY010000091        10803 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000091 DANKAY010000000
VERSION     DANKAY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..826
                     /locus_tag="PWY98_002253"
     CDS             <1..826
                     /locus_tag="PWY98_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2037989.1"
                     /translation="KDDVLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFK
                     FANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYS
                     LITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSS
                     NAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSH
                     MLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(908..2680)
                     /locus_tag="PWY98_002254"
     CDS             complement(908..2680)
                     /locus_tag="PWY98_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037990.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(2683..4395)
                     /locus_tag="PWY98_002255"
     CDS             complement(2683..4395)
                     /locus_tag="PWY98_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2037991.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4510..5178
                     /locus_tag="PWY98_002256"
     CDS             4510..5178
                     /locus_tag="PWY98_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2037992.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5245..6033)
                     /locus_tag="PWY98_002257"
     CDS             complement(5245..6033)
                     /locus_tag="PWY98_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2037993.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6116..7588)
                     /locus_tag="PWY98_002258"
     CDS             complement(6116..7588)
                     /locus_tag="PWY98_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2037994.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(7718..8911)
                     /locus_tag="PWY98_002259"
     CDS             complement(7718..8911)
                     /locus_tag="PWY98_002259"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2037995.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(8909..8988)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9177..9248)
                     /locus_tag="PWY98_002260"
     tRNA            complement(9177..9248)
                     /locus_tag="PWY98_002260"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9213..9215),aa:Glu,seq:ttc)
     gene            complement(9260..9333)
                     /locus_tag="PWY98_002261"
     tRNA            complement(9260..9333)
                     /locus_tag="PWY98_002261"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9297..9299),aa:Ile,seq:gat)
     gene            complement(9346..9416)
                     /locus_tag="PWY98_002262"
     tRNA            complement(9346..9416)
                     /locus_tag="PWY98_002262"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9382..9384),aa:Gly,seq:tcc)
     gene            complement(9421..9493)
                     /locus_tag="PWY98_002263"
     tRNA            complement(9421..9493)
                     /locus_tag="PWY98_002263"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9458..9460),aa:Phe,seq:gaa)
     gene            complement(9497..9569)
                     /locus_tag="PWY98_002264"
     tRNA            complement(9497..9569)
                     /locus_tag="PWY98_002264"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9534..9536),aa:Asp,seq:gtc)
     gene            complement(9588..9661)
                     /locus_tag="PWY98_002265"
     tRNA            complement(9588..9661)
                     /locus_tag="PWY98_002265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9625..9627),aa:Met,seq:cat)
     gene            complement(9674..9763)
                     /locus_tag="PWY98_002266"
     tRNA            complement(9674..9763)
                     /locus_tag="PWY98_002266"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9725..9727),aa:Ser,seq:tga)
     gene            complement(9786..9857)
                     /locus_tag="PWY98_002267"
     tRNA            complement(9786..9857)
                     /locus_tag="PWY98_002267"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9822..9824),aa:Glu,seq:ttc)
     gene            complement(9879..9966)
                     /locus_tag="PWY98_002268"
     tRNA            complement(9879..9966)
                     /locus_tag="PWY98_002268"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9930..9932),aa:Ser,seq:gct)
     gene            complement(9991..10064)
                     /locus_tag="PWY98_002269"
     tRNA            complement(9991..10064)
                     /locus_tag="PWY98_002269"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10028..10030),aa:Ile,seq:gat)
     gene            complement(10078..10148)
                     /locus_tag="PWY98_002270"
     tRNA            complement(10078..10148)
                     /locus_tag="PWY98_002270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10114..10116),aa:Gly,seq:tcc)
     gene            complement(10150..10225)
                     /locus_tag="PWY98_002271"
     tRNA            complement(10150..10225)
                     /locus_tag="PWY98_002271"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10190..10192),aa:Asp,seq:gtc)
     gene            complement(10244..10317)
                     /locus_tag="PWY98_002272"
     tRNA            complement(10244..10317)
                     /locus_tag="PWY98_002272"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10281..10283),aa:Met,seq:cat)
     gene            complement(10330..10419)
                     /locus_tag="PWY98_002273"
     tRNA            complement(10330..10419)
                     /locus_tag="PWY98_002273"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10381..10383),aa:Ser,seq:tga)
     gene            complement(10443..10516)
                     /locus_tag="PWY98_002274"
     tRNA            complement(10443..10516)
                     /locus_tag="PWY98_002274"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10480..10482),aa:Ile,seq:cat)
     gene            complement(10530..10603)
                     /locus_tag="PWY98_002275"
     tRNA            complement(10530..10603)
                     /locus_tag="PWY98_002275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10567..10569),aa:Met,seq:cat)
     gene            complement(10631..10703)
                     /locus_tag="PWY98_002276"
     tRNA            complement(10631..10703)
                     /locus_tag="PWY98_002276"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10668..10670),aa:Ala,seq:tgc)
     gene            complement(10723..10796)
                     /locus_tag="PWY98_002277"
     tRNA            complement(10723..10796)
                     /locus_tag="PWY98_002277"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10760..10762),aa:Pro,seq:tgg)
BASE COUNT         3363 a         2390 c         1890 g         3160 t
ORIGIN      
        1 aaaagatgat gtacttgtaa aaaacgttaa tagtaatctt atacaagata tgctagatta
       61 ctactatttt gaaaaaaatt tatcatataa ttatgtaata catttaaaaa cttttacagg
      121 aatgattttt aaattcgcca accgtcgata tggattaaaa tataatcccg taaatggggt
      181 ttctttagca agaaaaccta agacaaacga agaaataaaa aaagaaaaag atggttattt
      241 atcaaaagaa gaagttcaaa tgattgctag taaacaaaaa agttcacatc aacagtcacg
      301 ttattcttta attactaaat ttttgttttt aactggacta cgttatggtg aattgatatc
      361 tttgactgaa tctgattatg atggtaataa aattacggtt tctggtactt atgattacga
      421 gttaaaaatt aaaacaacaa ctaaaaatac aggttcttat agaaccattg aattatcaag
      481 taatgctaaa gaaatcatag atcaattaat tgaagaaaac aaattaatta agaataataa
      541 agataaatac attttcatct ctaaaaatgg taacccgata tctattcaag catataatca
      601 atctttaaga gcagtatcca aagaattaaa cctagataaa aaagtttctt ctcacatgtt
      661 aaggcattcc cacatttctc tacttacaga attaggaatc ccattaaaag ccattatgga
      721 tcgtgtagga catgaagatt caaaaacaac attaaagatt tatactcata caactaaaaa
      781 tatgcaaaat caactagtag aaaaactagg aaaaatagaa atttaaattg ccccttttct
      841 gccccttata aaaacaaaca acataaaata tataaaaaga accccgttat aacgaggttc
      901 ttttaattta actttcaaat acaaattgat tatgataaag ttccgcataa aatccatttt
      961 ctgctaataa ctcgtgatga ctgccttctt caatgacttc cccatcacgc aagacaacga
     1021 ttctatctgc attcaagatt gttttcagcc ggtgagcaat cacaaagctg gttcttcctt
     1081 tgattgcttc atccattgct ttttggatcc ttgcttctgt taccgtgtct acattacttg
     1141 ttgcttcatc caaaatcaat aaagatggat ttgtgatgat cgttcgagca atactgatca
     1201 actgcttttg acctgtactg aataagttat tttcttcgga tatctctgta tcataacctt
     1261 tttctaacgt catgatgaag tcatgaatat tcgcttgttt tgctgcattg atgacttctt
     1321 catctgaagc gtccggtttg ccgaatgtga tattgtcttt gatcgttccg gagaacaata
     1381 cagattcttg caaaacgatt cctacatgtc tacgtaatgc atcaagatca taatctcgga
     1441 tatctattcc atcgatgact actgaaccat cattgacatc gtaaaaacgg tttagcagat
     1501 tcatgatcgt cgttttcccc gatcctgttg gaccaacaag agcaaccatt tctcctttgt
     1561 ccacattgat cgagacatct ttcaagacta gtttttctgg agaataacca aaatctacgt
     1621 gctgcaacgc aagacctttt tcaacaccat caaatgcttt acagttttct ggtttgactt
     1681 catctttttc atcaaatatt tcattcaatc gtcttgcccc tgtaatcgct aattggatca
     1741 tgctgtatcc agatgaaatc tgcatcagtg gttggtaata ttgttgtgaa tactggacga
     1801 atgtcacaac taagcctaat gccaccgaac gttccaaatc accatttaat gctaaccagc
     1861 cgccgaaaaa gataactatc gctgtattga ccagtgacat tccttgcatc atcggaaata
     1921 gtagaccaga ataagcttgt cctttgaagg ttgcttcgcg aacactttga ttatgctcta
     1981 agaatccttc aatcgtttct tcttgtaagc catttgtgat gatgacacgt tgtccgctga
     2041 ttttttcatc catataacca tttagtttcc ccacttcatc ttgctgaaga tccacatatt
     2101 tccgtgcttt gcggataatc aagacagcta tcaaaacggc tactggagtg gaagcaattg
     2161 tagcccaagc taactctaca ttttggcgga acatcatgat caagatcccg acaagtaaaa
     2221 cagcattcgt gatgacttga agcaaagctt ggttcaaact attttgaata ttatctaagt
     2281 cactggtaaa acgacttaaa atttcaccgt cttgatgaga atcaaagaaa cggatcgtca
     2341 atttttccaa tttgttgaat agaccgatac gcattcggtt cgttgacttc cctactactt
     2401 gtgtgaacaa aatactatag ataaagttgg ctgcactagc tagtacataa aacagcaaga
     2461 gtttccatat tacagaaaca aatttgcttt tatcgtctac gccttgcatc aagcctccta
     2521 cgtaattagc taattcttga aaagcttccc cgatgtattg aggtgccttt acttgtagat
     2581 aggtagcaat gatcactgtg ataaagataa agaaaaagga gagtttgtac cgcttcaaat
     2641 agtgatagaa aaatttactt gcttttatta gatcagtcat acttactcct ccgttccttt
     2701 ttgagtttca aagatttcct gatagacacg gtttccagct accaattcct catgggttcc
     2761 ttcgccgact aatctgcctt catccaatac taagatgcgg tctgctttga caacagaagc
     2821 aattttttgt gcgatgacaa tcgtcgttgt tcccttcaaa tctcgatcga gtgcttctcg
     2881 gaccagcttt tcagagcgag catccagtgc actcgtactg tcatctagga tcaagatctt
     2941 tggattaccg ataacgccac gagtaatgga taaacgttgc ttttgaccac cagaaaagtt
     3001 actgctacgt tcttctattg gtgcttcgta acgatcagat aacttttcga tgaattcttt
     3061 tgcttgagca atagaagaag catgttccat ctctttttct gttgcattct ttttcccttg
     3121 gcgcaagttt tgagcaatcg ttcctgagaa taaaattgct ttttgcaaaa caaaggaaac
     3181 cgtttgtctt aaactctttt catttacttg acgaagatct acaccaccta cttcaacttt
     3241 gccttctgtc ggatcaaata gacgagggat caattgtgct agtgttgact tcccggcacc
     3301 agtcgccccg acgataccaa ccatttctcc aggtttgata gaaaagctga tatctttaag
     3361 cgtatctgta tcctcaccag gataacgaaa agaaacatgg tcaaaagtga cagaacccgt
     3421 caactcttgt tctggaacgt ctaaataagt aagatctggt tcagcatcta aaatttccgc
     3481 aatccgcttc agcgatacgg ctgctcttga agtcatcatc atcatcattc cgccaataat
     3541 gattgccatc ataatctgca tcaagtagtt catgaatgaa gcaattccac caattaacgt
     3601 aggatcatct ttaacaagat cgctcacaaa aaagatcgcc ccaaccacag ccaaattggc
     3661 agccagcatg aaggacggga tcatcacaga aaataatgta ccgactatga tgttgtgttt
     3721 tgttaagtcc tcacttactt tgctgaatga cttcaattgg ttttcttctt gtacaaaaga
     3781 tttcactaca cgaatcccta gtaagttttc ttttgcaatg ctattcacac gatcaatcag
     3841 tttttgaatg atcatgaagt gcttgcccat ccttgtaaat gaaagtgccg taatcagaaa
     3901 taccgtaacg ataagcgcta cgatgatcca ccataattgc ggcatcgtga tcatagctaa
     3961 aatgaaggca ccaataaata agaatggaat tcgaaaaagt gattgcaaag aaatcatcac
     4021 taaattttgg atttgcgtga tatcatttgt tagacgaaca acaagatttc cagcagagaa
     4081 ctgttcgata tttccaaatg aaaatgtctg gatctttcga aatgcttctt ctcggatatc
     4141 tgcacttacg ccttgagcta cttttgcaga gaaaatcgtg ttcgttacac ctgcggctaa
     4201 tcctagcaaa gccagactga tcaggtaaat accaattgtt ttcatttcat cattatcttc
     4261 tgtaataata gcttccaata cttgctgtag taacttaggt tgccataatg ctgctgcaac
     4321 catgactgct acggacagca aggaaataaa aacagctact ttatattttt tcgcatgtcg
     4381 taagattaat ttcattcgtt gattacaccc ctttgtttaa attgacagga ctaatttaac
     4441 actgtaaaat tagaccgact aacaatttac catgtttaaa aagataatgc aatacacgag
     4501 gttgataaaa tggaaataaa gctttctcaa gcaatcatca ttcaggccgc ctttgatatc
     4561 ctggctgaaa cacaagctct ttcgcagctt tccatgcgaa acatcgcaaa cagaattcat
     4621 gttcaagcac ctgctttata ttggtatttc aaaaacaaac aacagctctt gcaaataatg
     4681 gctgaaacaa tggaagaaga attagcgctt ccagatccaa ctttgccttg gaatgaacag
     4741 ttgctgcagt atatggaaaa ttactatgac ctttatacac gtttcccttg tggggcagaa
     4801 ttagaaatca atactgtgcc cgcttttccg agtcgattag aacatttgga gcaaatgatc
     4861 caattacttg ttcgtgaagg attttcagtc acccaaagtc atcaggccat tgcctctctc
     4921 cacaatttat tgattggaca gttaatggat cagcagcatg aacagcagct tcgacaaaaa
     4981 attatggacg gaaatgaatt tttgaaagaa acagttctat ttatgcgtaa ttatgtagtt
     5041 gaaaatcaat tagagggatt taaagaaagt atgcagtccc atgcagaatt gaatgaaaaa
     5101 gcactctttc taacgagtgc acaaacctat attgaaggct tatgccaaca aaataaaaga
     5161 agagaaaacg agcagtaaaa aagctcctat agagatcact atgtttgtga tctctatagg
     5221 agctatcttt ttaagactct ttttttattt ttcttccatt agccacgcca atgcacgttg
     5281 aatccattgg tcccagaaga tccagttatg atcgccaggt ccttgttcaa aggtgaccgc
     5341 caagccttgt tcctgcatag cagataccat ttcctgattt gcctgataaa gaaaatcaga
     5401 ttccccacaa gccatataaa atcttggaac tggttttccg ctttttacta gttctttcaa
     5461 taagtattgc ggatcatttt ttgaaccttc gatttgatcc aatggtccaa agatcccttc
     5521 ccaaaaagat gctttacgaa ccatcaaaag atcatccatt cgatttccta cagaaacagc
     5581 gcctgaaaga gagattactg ctgcgaaatt ttctgatttt gcaaggccta gttttaacgc
     5641 gccatatccg cccatcgata atccagcggc atatgttttt tctctttttc ttgttaattg
     5701 cgggaataat tcatgacaaa tctcaggtag ctcttctgca atgaatgtcc aatacttcat
     5761 gtcataaact gtatctgtgt accagccaag atcagtagat ggcatgatga cagctaatcc
     5821 gtagtttgct acataacgtt caatcgacgt acgtctttcc catacactat gattgccacc
     5881 cattccatga agtaaatata aaacaggtat atccgttgtc tcttgctctg aactggtgcc
     5941 aatcaccttt tttgttgttt gcggtagtat cacatttaga ttgacttcca tttctaacac
     6001 attcgaataa atatttgctt gaataaatgc catattttcg ccttccttag taagaagtta
     6061 tttttgaata actttaaatt tgaatgattc ggctacgtac atatcttaac cgaaactact
     6121 gttttatttt accacttttt tcatgaacga aaagcgttaa gaccaaagca atgatcacga
     6181 gtaacaagcc aactagataa acggaatgta acccatcata aagaattttt cttaatggta
     6241 acaaaatatc cgcatcaata tatttagccg tttgtggatt caccagttta ttcatgatat
     6301 ctgcatcatt tgttaaattt gctgctgcaa gttctcgttt agttacccca tttatcaata
     6361 aaccaaaaat agaaaccatg actgtttgac caattgttcg aactaacgta ttaaaagaag
     6421 ttgctacacc aagctggtca cgagatacac tattttgtgc actgacagtc gaggtagtca
     6481 tggtcacacc taagcctata ccgatgaccg ttgatatagc aaaaaaccat aaatagttac
     6541 ttctgaaagg cataagatac aaccaagcgc ccccaatagc tgcgattagt agtccaattc
     6601 tcaagaccca atgagttttc atttgagtca tccacttgct tgcaaggaaa gaaccgacca
     6661 tccagactag tgataatggt gctaatacca gcccaccaat tgctgcattc agccctaata
     6721 caccttgcat ccacataggg atataaacat cgattcccat caaaaaacca ctagccaatg
     6781 ctgcagccaa gttgaccaca acaaatagtg gatttttgaa taaatgcatc atgatgacag
     6841 gatctttagc tcttttttcg gcaaaaacaa aaatacctaa cattacgata ctacctagca
     6901 gcaaagcaga aacaagcgga ctcattcctt cgtctcctaa taattggaac cctaacagca
     6961 atgagacaag catggccatc aaactaaaac taccaaaaat atccattggt ttagactcat
     7021 gtatgatttt tggttcattt aaatatagcc aaatcaaaat aattaataga attccgatcg
     7081 gcacattgat aaaaaagatc cagtgccagc tgatcgtttc aacgatgaat cctcccgcaa
     7141 gcggtccaaa aacactggca atcccccagg ctgaactatt caaacctaaa acttttgctc
     7201 gcttttcaat agaataaaga tcgccgatga tcgtcaaagc tactggcatc attgccccag
     7261 cacccatccc ttgaatcgca cgagcagcaa tcaacccaat catgctttga gataaaccgc
     7321 aaaataacga accgataata aacaataatg tcccaaaaat aaagattggt ttccgtccca
     7381 ctttatccgc aagttttccg taaatcggag taagcatagc gtttgtcagc aaataaatcg
     7441 aaaataccca gttcatgatt tccattccat gtaatgaacc gacaatcgtt ggcatagcgg
     7501 ttgttacgat tgtaccttca atagccgtca tgaacgttgc aatgaaaata ctcactgtga
     7561 ccaaagtaac atttgtttct tttttcatct tattcctccc tttcattatg acaggatcta
     7621 aaaatagata aataattact atctttttca agaggttaac tgccataaaa aaagctggaa
     7681 caaccaccac ttggcgatcg ttccagctga atataactta ttttgctaaa cttgctttca
     7741 acgcatctac tttgtccgta tgttcccaag gcaaatctac atctgtacga ccaaaatgac
     7801 cgtaagcagc tgtttgtttg taaatcggac gacgcaaatc aagcatctcg atgattcctg
     7861 ctggccgaag atcgaagttt tctctcactg cagcaatcag ttcttcttcc ggaaccgttc
     7921 cagtaccgaa agtattgatt gagatcgaaa caggttgagc aacaccaatc gcataagcta
     7981 gctgtacttc tgctttgcga gcaagccctg ctgcaacaat gtttttagca atataacgtg
     8041 cagcatagct ggcagaacgg tcaacttttg tcgcatcttt accagaaaaa gcaccgccac
     8101 catgacgagc ataaccgcca tacgtatcaa caatgatttt tcttcctgtt aatccggcat
     8161 ccccttgagg accaccaatg acaaatcggc cagtcggatt gatataatat ttcgtttgat
     8221 catctaataa ttcagctgga ataacttcct tcactacttt ttcgatgaca tcatgacgaa
     8281 ttgtttcatt atctactgca tcatcatgct gtgttgaaat aacgattgta tctacgcgtt
     8341 ccggttgtcc ttgatcatca tattcaaccg ttacttgaga ttttgcatct ggacgtaaat
     8401 acgttaattc atttgattta cgcaaatctg ccaagcgtcg taccagacga tgactcaaag
     8461 cgattggtaa aggcatcaat tctggtgttt catctacagc aaacccaaac attaaacctt
     8521 ggtcaccagc accaatctca tctaatacgt cttttttatc ttcatcccgg atctcaagtg
     8581 cttcatcaac tccttgagca atatcaggag attgttcatc aatcgcaacc aatacagctg
     8641 ctgtatctcc atcaaatccg aattttgcac gtgtataacc aatttctttt attgtttccc
     8701 gtacgacttt ttgaatatct acatatgccg tcgtagatat ttctccaaat actaaaacaa
     8761 gaccagttgt tacagacgtt tcacacgcta ctcgtgctgt tggatcttgt tttaaaattg
     8821 catccaagat tgcatcactg atttggtcag caattttatc tggatgtcct tcagaaacag
     8881 attctgatgt aaataagtgt ctttctacca ttataatgat tccccctagt taaattcggt
     8941 tacaaggcat ctccgtcctg cggatccttt cgggaacttg caacgaacca agtatagcag
     9001 ataaaacagt tttctgctcg tattttttca tttttttgct taaaaaatat aaaaatattt
     9061 tacattagaa ctgtccaaaa aagcttagca caatcatcca taaagataac tctgctaagc
     9121 tttttaaaat cattctttca gataaaaaga aaaagatact caaaaagtat cttccatgac
     9181 ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc
     9241 aacgggccaa taaaataaat gggcctaaat ggactcgaac catcgacctc acgcttatca
     9301 ggcgtgcgct ctaaccagct gagctatagg cccataaaaa ctaaaagcgg gtgacgagaa
     9361 tcgaactcgc gacaacagct tggaaggctg tggttttacc actaaactac acccgcataa
     9421 tggcgcggga cagaatcgaa ctgccgacac atggagcttc aatccattgc tctaccaact
     9481 gagctaccga gccaaacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc
     9541 aggcatgtta acccctacac cacggaacca atgtttttag tttttaattg cgggagcagg
     9601 atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc tccatcccgc
     9661 gataatatga aaaaggagga taagggattc gaacccttgc acggttttac ccgcctgacg
     9721 gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatgtaaa actatattaa
     9781 attaatgacc cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac
     9841 cgcttgacca acgggccagg taaaacaaaa tattgaaacg gagaaggagg gatttgaacc
     9901 ctcgcgccgg tttcccgacc tacaccctta gcaggggcgc ctcttcagcc acttgagtac
     9961 ttccccaaaa ccaataagat tttgtactaa tgggcctaaa tggactcgaa ccatcgacct
    10021 cacgcttatc aggcgtgcgc tctaaccagc tgagctatag gcccataaaa aactaaaagc
    10081 gggtgacgag aatcgaactc gcgacaacag cttggaaggc tgtggtttta ccactaaact
    10141 acacccgcat ggcggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
    10201 atgttaaccc ctacaccacg gaaccagtgt ttttagtttt taattgcggg agcaggattt
    10261 gaacctacga ccttcgggtt atgagcccga cgagctacca gactgctcca tcccgcgata
    10321 atatgaaaaa ggaggataag ggattcgaac ccttgcacgg ttttacccgc ctgacggttt
    10381 tcaagaccgt tcccttcagc cggacttggg taatcctcca tatgaaactt acaacgccac
    10441 aatggacctt gtaggactcg aacctacgac cggacggtta tgagccgtct gctctaacca
    10501 actgagctaa aggtccaggc tcttaaaaat cgcggcggag gggatcgaac ccccgacctc
    10561 ccgggtatga accggacgct ctagccagct gagctacacc gcgaaaaaga tattcattat
    10621 ttaaatgaaa tggagcctag cgggatcgaa ccgctgacct cctgcgtgca aagcaggcgc
    10681 tctcccagct gagctaaggc cccgtgttta aatttataat tatcgggaag acaggattcg
    10741 aacctgcgac cccttggtcc caaaccaagt gctctaccaa gctgagctac ttcccgttta
    10801 att
//
LOCUS       DANKAY010000092        10598 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000092 DANKAY010000000
VERSION     DANKAY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..419)
                     /locus_tag="PWY98_002278"
     CDS             complement(<1..419)
                     /locus_tag="PWY98_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037996.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTAN"
     gene            587..904
                     /locus_tag="PWY98_002279"
     CDS             587..904
                     /locus_tag="PWY98_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037997.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            837..1316
                     /locus_tag="PWY98_002280"
     CDS             837..1316
                     /locus_tag="PWY98_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDL2037998.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1333..1509
                     /locus_tag="PWY98_002281"
     CDS             1333..1509
                     /locus_tag="PWY98_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2037999.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1766..2197
                     /locus_tag="PWY98_002282"
     CDS             1766..2197
                     /locus_tag="PWY98_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038000.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            2203..2520
                     /locus_tag="PWY98_002283"
     CDS             2203..2520
                     /locus_tag="PWY98_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL2038001.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2639..3325
                     /locus_tag="PWY98_002284"
     CDS             2639..3325
                     /locus_tag="PWY98_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2038002.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            3300..4649
                     /locus_tag="PWY98_002285"
     CDS             3300..4649
                     /locus_tag="PWY98_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2038003.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4640..5113
                     /locus_tag="PWY98_002286"
     CDS             4640..5113
                     /locus_tag="PWY98_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2038004.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5088..5615
                     /locus_tag="PWY98_002287"
     CDS             5088..5615
                     /locus_tag="PWY98_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HDL2038005.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            5630..7942
                     /locus_tag="PWY98_002288"
     CDS             5630..7942
                     /locus_tag="PWY98_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2038006.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            8189..8506
                     /locus_tag="PWY98_002289"
     CDS             8189..8506
                     /locus_tag="PWY98_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDL2038007.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            8503..8664
                     /locus_tag="PWY98_002290"
     CDS             8503..8664
                     /locus_tag="PWY98_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2038008.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            8667..8966
                     /locus_tag="PWY98_002291"
     CDS             8667..8966
                     /locus_tag="PWY98_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038009.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            8966..9280
                     /locus_tag="PWY98_002292"
     CDS             8966..9280
                     /locus_tag="PWY98_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2038010.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            9273..9803
                     /locus_tag="PWY98_002293"
     CDS             9273..9803
                     /locus_tag="PWY98_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2038011.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            9800..10069
                     /locus_tag="PWY98_002294"
     CDS             9800..10069
                     /locus_tag="PWY98_002294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038012.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            9999..10202
                     /locus_tag="PWY98_002295"
     CDS             9999..10202
                     /locus_tag="PWY98_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2038013.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            10199..10486
                     /locus_tag="PWY98_002296"
     CDS             10199..10486
                     /locus_tag="PWY98_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038014.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT         3814 a         1732 c         2362 g         2690 t
ORIGIN      
        1 taattagctg ttcttatgac cattacaagt ttttcaccag gtaatatatc tttcatatct
       61 aaaacttctg aaaaagcgac tgagtagcca ggagagattg cttgtgccaa cggtatagga
      121 gataaaaggc tacttttatg aatgcttccc atatcaaaac cattggcgtg ataatgtgaa
      181 caattaattt tcttaccgtt gtaaagatac gtacattcga taattgagtt tggcattttt
      241 gatgggtttg caattacgcc agtaagaatc attcgattac ttctatcatg cttaactaaa
      301 aaataggaga gcgtaatttt tcttttatct ttaaataagt tatagaggga taaaaaaaat
      361 cctgaaatag aaatgattaa agtaatagcg acttggtaat cgctcaaaat ttttatcaag
      421 taattcacca cctttttact caaattatat caaaaaggag agaagaaata atgcaagaat
      481 tagtaatttt gaaaaataaa aaaagacacg aacgccggca agcatgatcg tgtcagggaa
      541 ataaactatc tttctatatt ttaccacaga aaggaatgaa ccgtaaatgc taattgcaac
      601 ggatacacta gaccgcatct ttttaaaaga cgaatacaaa ctgcgcaata tagatgcgtc
      661 aggaatttta gtatttgatc tttatgacaa tggaaaaatt ggtatctatc aagcaagtga
      721 tattgaagaa acaaaccttg ctttcgaaca aattgatgat tctgtggaat tggatttaga
      781 tgaggcaatc ctagcttttg aacaaattgc aaaattatta aaggaggcac aaaagaatgg
      841 caactcttta ccaactcagc gagtcatata tcaaagtcct ggaactggca gaacaattgg
      901 atgaagaaat tcttcgcgat actctcgatt cgattaatga agcgatcgag tataaggcag
      961 aaaacttagc aaaaatagtt aaagaagtag aagggaaagc tgagttaatc gatagtgaaa
     1021 ttaaacgtct gcaggaacgt aagacatcgc ttttgaacaa tgctaagagt atcaagcact
     1081 atttacaaga ggaaatggaa aagactggta aaacgaagat taaaggtgaa ttattcaaca
     1141 ttggaattca aaataatcca gtatcggtca atgtaatcaa tgagaactta attccaaaag
     1201 gattctttac ccctgtgcct cccaaattgg ataaaaagca attgaaggag gaactgaagc
     1261 acggagatat tcctggtgct gaactcgtcc aaacaaaagg tttgaggata agatgatgaa
     1321 ggaggagaaa taatggaaca cgaaaacgta aaagacgctt tgaaagctgc tatcgaaatc
     1381 gcggaagcta aaggaataaa ggttgacggc aaaccagcaa ctgtccatga cattcaaaat
     1441 ttgacgaaag aacatcttta ttttatcgcc gacttgcttg gtttgtcgga attgtattta
     1501 gacaaataac cccacacgga aggagtggct agggtggttg tattacaagt aatagtctgc
     1561 gtaacattca tttggtcgtg cataccggtt tgtatatata tcatgaagaa gcttcgccct
     1621 gagtcttaca aattatacgc aatttacagc ataacagtag ctcttgcatt tacattgttt
     1681 gcaatttcta ggcttccgtt ttaatcgtgg gaatgaattt ttaatgaact aaattatcta
     1741 taaagcaaaa aaaggagaaa caacattgaa aaacaaacta gcaaaaacaa cagcaatcat
     1801 cggactagca ctaggtagcg gcgttatcgg ctacgcagca agcaatgcgt ttcaagattt
     1861 ggacacgatc aaagcaaatt tcaacacagt cctacaatac ggtcaaacga aatcgcaacg
     1921 tgtgtcagaa cttgaatcac agctatccaa caacactcgc acacaggagc agctgaaagc
     1981 cgaaattgag caaatcaaat cggacaagca gaaggaaatt gaagccaagc aacgagaagt
     2041 cgaacaaaag caacaggaga tcgctacaaa gcaacaggaa gccgatagct tgcgccaaca
     2101 actatccaca gtgcaaaacg acaaggagca gttagaacag cgcgtgagcg agttacggca
     2161 gtatacggat caaaaggtag gggagttggg gaaatgaaag agttgactat ttatgaccca
     2221 aatatttcat gggctaaaca cacaattaaa gtttctttta tgatttgggg ttacaaagga
     2281 tatgttactt acaaagtggg cggtaacact aagggtttat cattaatagc tattgattca
     2341 gatgatctat atgatgctaa tttcgaggat aacccagtta atttccgtga ccttgatgaa
     2401 gattggttct ctatggaact tactaacgat aaaggcgaca gtacacttgt agaagatgaa
     2461 ttcgacagac taggtgatta tattgtcggt gttgaaatta ttgcgcacga accagagtag
     2521 gagagtgaat acaaaatgat taaaagaatc actatatcag ttgaagaaga tagtggatca
     2581 acaatgcagc ataccgacaa tcgaaaaact tttttaacca atagaaagga gaattttgat
     2641 gcagattaag aaggcgacgg atatcgaaca aaccaaggga acatatttga tctacggtgc
     2701 tccaggaaaa ggtaaaactt caacggctaa atattttcca gggaaaacac tgatccttga
     2761 tattgatcgg acttcaaaag tccttagagg tgagaaaaat gtcgatatcg tatatatcga
     2821 caacgaagat acctggaacg actggggcaa cacgttggct gatttaacta ccaattatgt
     2881 aggtgtttat gacaacgtgg ttgtcgataa tgtcagcgaa ttggaacggt gtattctgtc
     2941 cagtttaggc gcagaaggaa aaaacaacgg cgttccttcg caaggcgatt atcaatatat
     3001 gcaatttaga atggtgaaca gcctacgcta tatgaaaaat ttagacagca accttatctg
     3061 gaccgcatgg gaagaaatcg atttgtggac ggattcggat ggatcttctt acaacatcgc
     3121 tttgccgcag atcaatcgaa agattcgaaa caatattttg ggattatgtg acgttgttgg
     3181 tcgattgatg gtaaaggaag acgatgaacg cggatttatt ttacaagcca cagattcaac
     3241 ctatgccaaa aatcaattgg ataatagatc tggctgtaag caaagtgagc tgattttaga
     3301 tggaattgta cgactaccag aatgatttag tcgatcgtgc aagacaagcc tatgttgatg
     3361 gttacaaggc accatgtatc gtatcaccgt gcggttctgg aaaatcagtc atggttgcag
     3421 agatcgctaa acgaaccaca cgaaaaggca acagagtgtt gttcttggtc catcgtcgtg
     3481 aattgattga tcagatcaaa gatacgtttc aaaagatggg cgtagataat cagcttgtaa
     3541 atttcggcat ggttcaaaca gtcgtacgac atcttaaaac catcaagaaa ccacaactga
     3601 tcatcactga cgaaaaccat cacgggttag cagcgtccta cagaaaaatc tatgagtatt
     3661 attcggacgt tccgcgatta ggttttaccg cgacacccat ccggttaaac ggtagtgggt
     3721 tgggggatgt gaacgacttg ctaattgaag gagtatcggc taaatggttg attgagaatc
     3781 atagattagc tccttatgaa tattatgcac ctaaattgat cgatacagcc gaattaaaga
     3841 aagcttcgac tggagatttt acaaagaaat caatggataa ggcagtgaag aatactattt
     3901 atggcgacgt gttgaaacat tataaaacgt tagcagaagg tgaacaggca atagcatact
     3961 gtcacagtat cgatgcaagt aaacacactg ctgaaatatt caacgatgct ggttataaag
     4021 caacacatat cgatgcaaaa actccaaaag atgaacgtgc agatattatc gaagcgttca
     4081 gaaatcatga aattaaaatc ctatgcaatg ttgatttgat cggagaaggg tttgatgttc
     4141 ctgattgttc gactgtaatt atgttgagac ctactcaatc gttatcattg tacattcagc
     4201 aatcgatgag ggggatgcgt taccgaccag ataaaacgtc aatcatcatt gaccatgtgg
     4261 gtaatgtgaa ccgttttgga ttgcctgata tggatcgtga atggagttta gatacaaaaa
     4321 agaaaacaaa aactgatagt gatttatcta tcgttcaatg cgcgttttgt tttggtgctt
     4381 acgaacggcc aaaaggcgat aatatttgtc cgtattgcgg agagttgcag ccgattgaag
     4441 aacgaaagtc agagttagaa atcgatgaat cggctgaatt aatgaaagtc ggagaaacga
     4501 aaattacttt gaattttgag aacaataaat attacaacat gacggaagat gaagcagaaa
     4561 gtatagagga cttatatgcc atagcaagag ctaaaggatt taaacctggt tgggcttata
     4621 tggctgctaa gaggaagggg tggttgtagg aatgggcggt atttttggta aaaaagcaga
     4681 agatgttcaa ggttcaataa taaatggcgt aacaataata ggtgatactg gaaaaagaga
     4741 tagttcaggc aatcaaattg taatcgctag aaataaaaat aacgaagttt ttgaatcttc
     4801 tctatctaat ataaaaagag gtgttgttaa cgggacaggc gaagagagtg gctacgttaa
     4861 tttaaaaaaa gctaatgaaa aataccatgt tgaaggaact tacgttaagg ctttgtctgt
     4921 cggtggaaca aaaggcgtta attgggataa acgaagaaat aaatggaaag cacgattata
     4981 tttcaaagga gaatatgtct tacaaaagga tttcaacacc aaagaagaag caatccaagc
     5041 acgacttgca gcagaagaaa aatatttcaa acctatctta gaaaaatatg aaaataaaaa
     5101 ggagaatgat taacatgaca ggatttaact tagacttttc aaaagcgcaa caagggaacg
     5161 aaatcaaaga cggtacgtac gaagtggtcg taaacaaagc tgtagaaaat gcaactaaat
     5221 caggcgcaga atttatcgat atcgatttga tcgttcgtaa cgatgtggat caaccgttcc
     5281 aaaataaaca cattttcgca aaaatctgga aagccaaagc taccggcaaa tataacgaag
     5341 gtatgatcat ggcaattgca caagcgctac agttggaaga cggaaaatca tataacgggt
     5401 ttgatgaatt gctggcggac tttgttttga aaaccgcctc agtacgcgtg aaaactgaag
     5461 agtcaaatgg atataaaaac gtcaatgtga agtcttggga taaaacgaac acacggggcg
     5521 taatgaatca ccaattcaaa aatggtgatg aaccatcctt cggaccggaa cgttcaagag
     5581 cgacaacggt tagaaatgac aatcttccat tttaattgag gtgtaatcaa tgtatgaaaa
     5641 aattccaaaa gagcttaaaa atctcaaaca atggtgtgtt tataagctag tttgggatga
     5701 aaagcggcag aagcacacga agatccctta taacgccaac aacgggcata aagccaaatc
     5761 caatgacgag agtacctggt ctgattttca gaccgctctc gctgctattg atcactacgg
     5821 aatgagtgga ctggggttct ttttcaaacc accttatttc ggcattgata tcgataatgc
     5881 tgaaggtgaa gttgagcgat ttaaatcagg tgatatagaa gaaaatatta tctatgaatt
     5941 tatcgaaagc atgagatcct atgctgagta ttctcaatca ggtactggca tccacattat
     6001 tgctcgcgga gaattgcccg gcggaagacg caggaaaggt gacgtagaaa tgtaccagaa
     6061 cggccgattc ttcgttatga ctggaaatgc cgcatcaaaa tatttagaaa taactgagcc
     6121 gaatccaaag gatatcaaac gcctatatga tcgctacgtt ggagatgaaa aaatcatcca
     6181 attcaaagaa gaaaatccgc tgatgaatac cgttgattta ccggttgaag aaattatcaa
     6241 acgtgctgaa tcgtcttccc aaggggcaag attcaaagtc tttatgaacg gcggttggga
     6301 agctttttat tcctcgcaat ccgaagcgga tatggcgttc gccaacgact tggctttttg
     6361 gaccggtaga gactttgaaa aaatggatga gattttcaga gcgtctgcaa tgatccgtag
     6421 caaatatgac caaaaaaggg gatcggcaac ttacggagaa tctctactaa ataaggcaat
     6481 tgctgataca aattccgtct acaaccccaa acggaaatct gattttaaga tttttatcaa
     6541 agaccaggaa cagccaaaag aggaaaaata ctattcgtac gacgacaccg gcaacgcgga
     6601 tcggtttact gatatctacg gaacgttagt caaatattcg tacattgata aatcttggta
     6661 ttactacgat gggaaagttt ggctacagga caataccggc gaagttcgga aaatgatcga
     6721 tacgacagta gacatcatgg gaaaagaacc gttaacgatc cctgaaggcg cagacgatga
     6781 aaccaaagaa gctttgatga aagcgaaaga aaaacacgtc aagcgttctc gcagcaatgc
     6841 cggaaaaaac gctatgatgg acgaattgaa acatcgacta tccgtattgc cggaagagtt
     6901 cgacaaagat aaaacgctgt tcaacacaca aagcggctat ctgtctttgc atgacggtct
     6961 tctacacgag catgagatag acaaaatgtt tacacgtgtt tcaaatgtcg agtacacaga
     7021 atcggtcgat tgtcctatgt gggaagaatt tattaatcag atttttgaca atgatcaaga
     7081 attaatcaga tatatccaga aatgtgtcgg atattccctg accggatcta ccagagagca
     7141 atgtatgttc atcctctacg gtcatgggtc aaatggaaag tcggtatttt tggaaattat
     7201 ttctgaattg atgggaaact atgcgatgac tatgcaggca caaacgatca tggtcaagca
     7261 aagccaatca tcagcaaaca gtgatattgc acgattgaaa ggtgcgcggt tggtcacttc
     7321 gtcagaaccg aatgaaggcg tccgactgga cgaaggtctg gtcaaacaat tgactggtgg
     7381 tgataaagtc acggctcggc acctttatgg aaaagaattc gagttcgaac ctgaattcaa
     7441 gttgtggctg gcaacgaacc acaagcccat catccgtggg accgatgacg gcatctggcg
     7501 acggttaaat ttaattccat tcactgtcca aatcccggat cacaaaaagg ataaaaatct
     7561 gaaatttaaa ttgcagacgg agttgcaagg gatccttaaa tgggcaatcg atggttgcct
     7621 gttgtggcag agagagggct tggagaaacc aaagtcggtt gttgcagcga gtcaggatta
     7681 tcgaaacgaa atggatcaaa taggtacatt tatcgaaacg tgttgcgaaa caggtccagg
     7741 actaaaaata tcaggcggag aattatacaa agtttatcgt gaatgggcat cagataatgg
     7801 cgagcatact ttcactaaca cgaagtttgg cagagagatt tcaaagaaat atagcaaaga
     7861 aaaagctggt ggatttatgg tttataaagg aatcacatta aaaccaagaa aatacgataa
     7921 cgttagagaa ctttttaaat aaatctggag ggttctggag ggttgagaac gaaaccctcc
     7981 aacccttaga aacgttgata tatcagtatt tatattatat ttttctttct ttctggaggg
     8041 ttttaaaaaa ataataaagt attaaagtaa aaaataaaaa gtaaattata taaaaaagaa
     8101 taggtttttg ggtctgaccc tccaaactct ccaccaaaat cttcctaaat gctttgatac
     8161 caacgattaa acagtctgga gggttacatt gaaatcagaa catcaaatac aagacgagat
     8221 cagaattgcc ttatcaaaac atggttgcac gatttttcga tccaatgcgg gacaagtgac
     8281 aacgatcgaa gggcggcaat tttacgggat gccaaaaggg ttcccagatc tatgtggaca
     8341 tcgtaattct gatgggaaag caatttatat agaagtaaaa aatgaacgcg gtaagttgcg
     8401 tgaagatcaa aaaaggtttg ctgaattttt aaacggtcaa cctgtactat acggtgttgc
     8461 acgatcagtg gaagaagcat tgaaaattgt ggaggaatca tcatgacaac agaagaagtg
     8521 attcaaatgc gtattcgaag ccttcagcgt gagattgacg atctggaacg gacaaaggca
     8581 gtgatggtca atgaaacggc gaggaaggca atcgatttgc acatagagaa tttaagaagg
     8641 gaaatccatc gattggagga atgagcgtgg ataagaaagc agcaatgaaa cgaatcatcg
     8701 aactgacaca ttctgagaat tggcaagaag acaaagaaat agttgcagaa gtccaaaagc
     8761 tcggtaaatc aatgtggagt gaaaaaacca aacggagaac accgagaaaa attgcaatct
     8821 ggcatggtga ccgaattcta gtaacaggta ctgctgaaca gttatctgaa attactggat
     8881 taagcaaaaa cattatctgg gatagagcta aacgcgagaa cgttgattcg aaaggtcgtc
     8941 aatttaaaca ttgggagaag aaataatgga ggaactaatc acaaaagtag agcagtgggc
     9001 aaaagataag ggattggatc aagctgattc cagcaagcaa atgttaaaaa cgatcgaaga
     9061 gattggggaa gttgccgctt ctctagctag aaaagatgaa tatggtttaa gagacggaat
     9121 tggagacgta gtagtaacct tgattatttt agctatgcaa aatgatatgg atttgtacga
     9181 gtgtctgaac caagcataca acgaaatcaa aggacgcaca ggaaaaatgg tagatggtgt
     9241 attcgtgaag tcgagtgatt tggaggacag cgatgaataa aaaagtatta attgataaac
     9301 aagcattgat tgatgaatta atgaaaatac ctggtgtggg atctaatagt gacgctttag
     9361 aaacgattaa acgtttccca tcgtatgaac cgcagaagcc agttgtgcct aagtttgtgg
     9421 cggaactact cgactattat cgtcagtcaa cagatgttga tttattagct ttattgataa
     9481 ctttccatga ttggtattat cgcaaaacta aagacggtga gcatgaagaa gcaattgatt
     9541 ggctagttga tcatcctgaa atttatatgc gtgcatggct gtttggctac gagctcgaga
     9601 aagagccgtt gtattgcgta ataatcaatg gtagatatct tgtcaaagta ttcagcaata
     9661 caaacgttgt catattagtt ccagcagatg aattttcaca gtacgttact caaaaatttc
     9721 aattaactga aaaacaaatc aaagcaattg atgaaagata ctggccgttt gctgtgccag
     9781 tggatgaggt ggtagaagga tgagcgaccc atacattact aaattagaaa agttgtccga
     9841 agattttgag aagaataaaa ataaactaac taaagataaa cttatagaac tggttaggtt
     9901 ggttatcaga ggagctatta caagagaaaa ggcgcagcaa aacatagaaa tttttgctcg
     9961 tagtgtccga gatggattag agaagaaagt taaacaaaat gaacaacaga caccgcagaa
    10021 taacaaaact aagaaaacgg gaactgaatg tactaaagac aaagtttgaa aaagaatatg
    10081 gaatttcagc agaagaaaca tataaagtgg caagtcagtg tgttgctgat gcgagcggcg
    10141 ctattcgtaa gtttggaatt tcgatattaa gtgatgatcg taaatgggag gaaaaagaat
    10201 gaaactaaaa gacggatttt actccagcag tcatggtatc ggcggtttaa tgctagatat
    10261 gccgacaaag aaccctaaaa cacgtgagaa accaaaattc aaagtcggtg acatggttcg
    10321 ctgtgaagca gaaggattca tctatccatt ttgtggatat gtagaacatc tctataatca
    10381 ctcagcaatc attcgtattg aaaacacgat ggaatgtgac aagtggttag cgaaaagcaa
    10441 agagaattta gcagtagcga gattggtgga tatggaggtt atataaacag cgaaaaaatg
    10501 tacttctaag agaaaaattt attttttata aaaataggaa agtatcatta tgaaaaagac
    10561 cacagagata aataaaccta ccacataaaa aatgaata
//
LOCUS       DANKAY010000093        10488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000093 DANKAY010000000
VERSION     DANKAY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            180..1076
                     /locus_tag="PWY98_002297"
     CDS             180..1076
                     /locus_tag="PWY98_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2038015.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1226..2167)
                     /locus_tag="PWY98_002298"
     CDS             complement(1226..2167)
                     /locus_tag="PWY98_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2038016.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2293..3414
                     /locus_tag="PWY98_002299"
     CDS             2293..3414
                     /locus_tag="PWY98_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2038017.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3672..5174
                     /gene="eat(A)"
                     /locus_tag="PWY98_002300"
     CDS             3672..5174
                     /gene="eat(A)"
                     /locus_tag="PWY98_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2038018.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5202..5768
                     /locus_tag="PWY98_002301"
     CDS             5202..5768
                     /locus_tag="PWY98_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2038019.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5979..7040
                     /locus_tag="PWY98_002302"
     CDS             5979..7040
                     /locus_tag="PWY98_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2038020.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7043..7939
                     /gene="murQ"
                     /locus_tag="PWY98_002303"
     CDS             7043..7939
                     /gene="murQ"
                     /locus_tag="PWY98_002303"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2038021.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7953..9935
                     /locus_tag="PWY98_002304"
     CDS             7953..9935
                     /locus_tag="PWY98_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2038022.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10000..10446)
                     /gene="nrdI"
                     /locus_tag="PWY98_002305"
     CDS             complement(10000..10446)
                     /gene="nrdI"
                     /locus_tag="PWY98_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2038023.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3430 a         1855 c         2261 g         2942 t
ORIGIN      
        1 agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa tgatttattt
       61 catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt tttactggct
      121 ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag gcggaaaaga
      181 tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt ttgccttcca
      241 tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc attaaatcag
      301 ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga tttgatatgg
      361 agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt ttttggaatc
      421 acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg gcaatcgatg
      481 agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat aatagttcat
      541 tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta ttttttggtg
      601 caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt ataaaaagag
      661 atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct ggatatctta
      721 cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg
      781 agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa aagaaactag
      841 tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat atttttttgc
      901 ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt aagccaatcc
      961 tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt atcactaacg
     1021 attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac aaataaaaaa
     1081 cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat ggcatcatga
     1141 gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca gctgctgatt
     1201 cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga
     1261 ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc
     1321 aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt
     1381 acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt
     1441 acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca aattcagata
     1501 cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca atattacaat
     1561 ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc caaatggaat
     1621 aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact tctacaactt
     1681 tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta
     1741 acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga
     1801 tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca tttagtaaac
     1861 acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata tcatctaatt
     1921 gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg atgatttctt
     1981 ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca gcaatcaacc
     2041 catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg acctcatttc
     2101 cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca gctattgcga
     2161 ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaatgga
     2221 agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag
     2281 tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag tgcttcttca
     2341 tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat
     2401 gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcagggga
     2461 acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat cagctattta
     2521 ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc attggggtta
     2581 gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta tgccttagga
     2641 gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac tttaggagga
     2701 agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc tttagaaggg
     2761 tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg aagaaacgtt
     2821 cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata tacaggtcaa
     2881 caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac gctagctttc
     2941 ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga ggtggattta
     3001 gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt tttgttggga
     3061 gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga aaaaaagatc
     3121 gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac gatccacgga
     3181 aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt
     3241 tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat tgtcttaagt
     3301 attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac attagaaaaa
     3361 acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa gtagttgaca
     3421 aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca
     3481 ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaagga
     3541 agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc tttttgtgtg
     3601 tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga
     3661 ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag
     3721 gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta
     3781 tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt
     3841 atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata
     3901 aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg
     3961 aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac
     4021 tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt
     4081 tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg
     4141 cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg
     4201 atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga
     4261 atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg
     4321 aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt
     4381 ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta
     4441 gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag
     4501 caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg
     4561 aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg
     4621 tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg
     4681 agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc
     4741 attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc
     4801 tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg
     4861 cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg
     4921 aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg
     4981 aattggctaa atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt
     5041 atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca
     5101 tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc
     5161 ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg
     5221 ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg
     5281 cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg
     5341 cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt
     5401 cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc
     5461 tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca
     5521 aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca aatggggagt
     5581 cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc gaagacgaag
     5641 aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat gcttattttc
     5701 atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa ttggtttctt
     5761 atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg cttttgctta
     5821 gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac ctttagaaat
     5881 gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat atactctagt
     5941 catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta
     6001 tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct
     6061 ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca tatcaaaagc
     6121 gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg gtcgatattt
     6181 caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct ttgaaagcaa
     6241 ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt gccgactggt
     6301 cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat atcgatgaat
     6361 tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc
     6421 ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag
     6481 gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca ctatatcaag
     6541 gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct ctagatctat
     6601 tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa aacgttcagg
     6661 agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg
     6721 aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat gcaagagatg
     6781 tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca agaaatacag
     6841 ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc tatatgggtg
     6901 agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta gtagctaaag
     6961 ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac tatcaattta
     7021 taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg
     7081 cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg ttcaactaat
     7141 gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc
     7201 agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt atatgggagc
     7261 cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt
     7321 agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga ctgtagctgt
     7381 cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc tgaatttgac
     7441 aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg
     7501 attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca ataaacaagc
     7561 cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag aatttttgac
     7621 aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata tgatttctac
     7681 tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg tcaaaccaac
     7741 aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag aagctgatta
     7801 cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat
     7861 gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag caaacggatt
     7921 tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg actttggaag
     7981 aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa agcattgtcc
     8041 actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat gtctctgaat
     8101 taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa gtcatcatcg
     8161 gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc gtgaaattag
     8221 gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct ggaaaagacc
     8281 gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta
     8341 aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt cctgcatttg
     8401 tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc acagccggaa
     8461 cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg aaaaacggga
     8521 tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt ggtgcaacac
     8581 caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat ccggaagcac
     8641 cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg
     8701 tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg
     8761 atgcggtaga tatcatcgtt acaccaactc tttcattgat tgccatcggt ctgattgaaa
     8821 ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt gggatcaact
     8881 gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta ttcttgccga
     8941 tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg attgatgaaa
     9001 ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag
     9061 cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag
     9121 ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta acattacctc
     9181 taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca gtgatcggaa
     9241 tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg atcccattga
     9301 tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat gctggtggct
     9361 tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa gaattagcga
     9421 ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa
     9481 gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat aaagcaagtg
     9541 atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca gaaaacggac
     9601 atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa cacgctattg
     9661 ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac acagttgact
     9721 taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc
     9781 ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca tccatgatcg
     9841 tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa
     9901 aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat agaaacgaaa
     9961 gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt tttctgctaa
    10021 aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat ctgccaagaa
    10081 aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat tattgaagtt
    10141 tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat gttccagata
    10201 ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat ttccgccgtc
    10261 taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat cactattttc
    10321 atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca ttttttcagc
    10381 atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata tatataaaat
    10441 gttcatggat catcctccaa ttctttcctt ttagtatagc atcttttt
//
LOCUS       DANKAY010000094        10365 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000094 DANKAY010000000
VERSION     DANKAY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(82..324)
                     /gene="acpP"
                     /locus_tag="PWY98_002306"
     CDS             complement(82..324)
                     /gene="acpP"
                     /locus_tag="PWY98_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2038024.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(446..1447)
                     /gene="plsX"
                     /locus_tag="PWY98_002307"
     CDS             complement(446..1447)
                     /gene="plsX"
                     /locus_tag="PWY98_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2038025.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1496..3532)
                     /gene="recG"
                     /locus_tag="PWY98_002308"
     CDS             complement(1496..3532)
                     /gene="recG"
                     /locus_tag="PWY98_002308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2038026.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3649..5331)
                     /locus_tag="PWY98_002309"
     CDS             complement(3649..5331)
                     /locus_tag="PWY98_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2038027.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5383..5745)
                     /locus_tag="PWY98_002310"
     CDS             complement(5383..5745)
                     /locus_tag="PWY98_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2038028.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6015..6203
                     /locus_tag="PWY98_002311"
     CDS             6015..6203
                     /locus_tag="PWY98_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2038029.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6441..9110
                     /locus_tag="PWY98_002312"
     CDS             6441..9110
                     /locus_tag="PWY98_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2038030.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9565..10311)
                     /gene="truA"
                     /locus_tag="PWY98_002313"
     CDS             complement(9565..10311)
                     /gene="truA"
                     /locus_tag="PWY98_002313"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2038031.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2825 a         2234 c         1863 g         3443 t
ORIGIN      
        1 cttttacgtg tcaaaaagat taaaaaaaga aggataaaaa gctgcatgca gctttttatc
       61 cttctttgct tttatttgaa attatttaag atggctgctg atataatcta ctgcgccacc
      121 gactgtttca atttgttcag cgtcttcatc tgaaatttcc gtaccgaatt catcttcaag
      181 ctctaaaacg aattccataa tactaattga atcagctttt aaatcatctt tgatgttcaa
      241 ttcatccgtc actttatcgg tatctatctc aaaatgattt gaaataattt tcgctacttt
      301 gttaaatact tcttcgcgag tcaacttttt cacctccaag aatcatacac ttgtcaatta
      361 cctacaccat ggctaatttt actggcattt ttcaggaatg tccacctttt tttcaatgga
      421 acggatcgct tcagatatcc aaatactatt cttcgctttt ttcaaattgt tcgactaatt
      481 gtccaactac atctgtctca agcattgtgt ggatttggcg gatcgtatag cgaacagcat
      541 cggggcctgt tgctccatga gttttaatga ctggtgcttt caagccaaac aaaacggcac
      601 cgccatgttt ggaataatcc atttctgctt tcaaagaacg taagccgtct tttaaaagca
      661 tagcgcccat ttttcctttg atcccttcat ttagaatagc agattttaat agattcatca
      721 tattcattgc tgttccttca atagatttca aaacagcgtt gcctgtaaat ccatctgtga
      781 ccactacatc agccacacca ttgagtagct cacgagcttc tacgtttcca ataaaattca
      841 agcttgtttc attctgaagg agttcatagg cttttttcgt cacttcacta ccttttgttg
      901 cttctgttcc gttgtttaag agtgcgacac gaggacgtcc gatacctcgg actttacgtg
      961 cataaaaaga acctaaaata ccgtattgca acaaatgttc tggtttgttt tctgcgttag
     1021 cgccaagatc cagcatatcg aaaccggcac cttctttccc tactactggc aaggtagaca
     1081 tcaagccagg gcgttcgata tttttgatac gtcctacgat gaacaaccct gctgctagta
     1141 gcgcgcctgt attccctgct gaaaatacag catctgcttc accgtttttc actgcttgtg
     1201 cagcaaggac cattgatgca gtcttttttc gtcgaattgc ttttactggc tcatcatcac
     1261 tattgatttt ttcgtctgta tggataatcg tgatgttttt ttcatttgtt aagtatttct
     1321 tgatttctgc ttcttttccg taaagtaaga attctacttc tggaaaatct tgctgcgcta
     1381 aagccacgcc ttctacaatt gcttgaggtg cgtgatctcc gcccattgca tctacggcaa
     1441 ttttcattgt ttccactcct ttttccgtca tcagtataaa gtatagcaca gtctgctaat
     1501 cgaaataacg attatcctga ttttctattt ggaggctttg cgctaaaggc tgatactcag
     1561 gaagcatctg ccaatccttt atttgccaaa gggctgcagc ctcttctctg gctacctcca
     1621 aaacatttcc atccgtcacc aagtcagcaa tcgcaaattg aggcagacca gattgacgga
     1681 agccaaatac ttcaccagga ccacgtaatt ccagatcttt ttcgcttagt acaaaaccat
     1741 tggtggtctc tgtcatgatt ttcatccgtt ccacacctag ttcattttta ggattagcaa
     1801 ctaagataca gtaggaagca tcacttccgc gtccaacacg tccacgaagt tggtgcagct
     1861 gtgccaatcc aaatcgatcc gcatccatga tcagcatgac ggtcgcgttc ggcacgttta
     1921 cccctacttc aattacagta gtcgaaacta atatctgcat ctggttgtct ttgaaggctt
     1981 ccatgattac ttctttttcc tgattcttca ttttaccatg taacaagcca acttcatatt
     2041 ttggtgcaaa tagttcacga agtttttcat agatttccac tgcatttttt acatctaatg
     2101 cttctgattc ttcaatcagc ggacagatga catacatttg atgtcctctt gccagttctt
     2161 ttttcgtcca atccagtaca ctgtccaatt gaggtgttct tacccagcgt gtttcgatcg
     2221 ggatacgtcc agctggaagt tcatcaatga tagatacatc catctcacca tatgctgtaa
     2281 tagctaatgt tctaggaatc ggagtcgctg tcataaataa gacatctggt cgccagcctt
     2341 tttcacgaag tactttccgc tggttcacgc caaaacggtg ctgctcatcc gtaataacta
     2401 gacctaaatt ctgaaattcg actccatctt ggatcaatgc atgtgtgccg atcacgacgt
     2461 taatttcccc ttgctctagt ttttcaagga gctcacgtcg ttcttttgct ttcgttgacc
     2521 ccgtaatcaa tgcgatggtt acttcattgg gatcaaacag ctgttgcaag ctttccatat
     2581 gttgttgtgc caagatttct gtaggcacca tcaacgcacc ttggaaacct gctgtaacgg
     2641 tagcgtataa cgcaatcgcg gcaaccactg tttttccgct acctacatct ccctgcaaaa
     2701 gtcgctgcat atgctgcgga ctgcgaagat cccgacagat ttcattcgtt acacgttttt
     2761 gtgcatttgt cagttcaaaa ggtagctttt gagtaaattg tttcaatttt tggacatcat
     2821 aaaggatctc caaaccattc gtttctgctt tttctgcttt tttcaatcct tgaagacgca
     2881 tttgaaataa aaagaactct tcaaaaacga cacgtcgttt ggcttgatgg ttttcttcgt
     2941 ggtcttttgg aaaatgcata cttcttacag cagaagctcg gtcaagcagt cgatatttct
     3001 ccacaaggtt gtttggcaga ttttcttcaa tcaatgtgcc gtattcttga aacccttttt
     3061 tgattagatc aactaatgtc gtttgacgaa ttgatttgtt gacgtgatat atcggcgaaa
     3121 agtcatccat tgtttgagaa cctaaaatct tcatcccgtt caaggcttta cgctttgcat
     3181 cccatttacc gtatacggcg atttcctccg ataaaatgac tttttctttt agataaggtt
     3241 gattaaaaaa agaaacgttg aacacatcat gttcttgcat catacgaaat tgcaatcggc
     3301 tttttttata accaaatcga ttcatcacag gaggcgagac tacgattcct ttcaaagtaa
     3361 ccttttcttg atcctggatc tcgtttagat ttctttcctg aatatcttcg tagcgaaaag
     3421 gataataagt cagtaagtct tcaatcgttt ggatgcccaa gctggcaaga ctagctgctc
     3481 ttttttctcc gactcctgcc agcatagtga ttggatcatc taaagaacgc ataaattttt
     3541 cctccctcct ttaacaaaaa aaggacttgg tcagcgatca tacctgatca ctaaccgaag
     3601 gccttttatg aatgaatact gtgttagaaa gatattctga cacagaaatt attcagcaga
     3661 aaatagataa ggatagacag gctgatctcc ttggtggatc tcgatttcca aatcaggatg
     3721 tttttctgtt agtttctcag caaattcatt tgcttgttca actgttcctt catcacctgc
     3781 caagatcgtt acgatctctg tgtcctcatt gatcatttgt tctagtgtac ttaacgctgt
     3841 tgctgtgata tctgggttag agatcaggat ttttccatcg atcatgccca aaaagtctcc
     3901 ttctttgatc tctacaccgt caattgaggt atctctgatc gcgtgagtga cttgaccgct
     3961 cacaacgcta gacaacattt ctgtcatcgt ttctttgttt tcttctaaag aagcttgatc
     4021 attgaatgaa agcatagctg tcaatccttg ggaaatcgtt cttgaaggaa caactgctac
     4081 tgggatatct gctacttctg ctgcttgatc agctgccatg aagatgttct tattgtttgg
     4141 caagacaatc actttatctg cgtttacttc ttgaatcgct ttaacaatgt cttccgtact
     4201 tggattcatt gtttgtccac cactgataac ataattagcg cctaaacttt caaacagctc
     4261 ttggacgcct ttaccagcag cgatcgctac gataccatac gggatacgtg gtatttcttt
     4321 tttcgcaaac tcgttcactt cttcatcgtg ttccaaaatc gtctcatgct gcaagcgcat
     4381 gttgtcgact ttgattttga ctaatgaacc gaatttttgt ccataattca agacttctcc
     4441 tgggttttct gtatgcacgt ggactttgat gatctcgtca tcattgacca caagtaaaga
     4501 atctcctata ccatctaaat agttacggaa ttcttcgtaa tcgaatgtac tatcgactgt
     4561 aggtccttct ccgattttta ccatgatttc tgtacagtaa ccaaaatgga tatcttctgt
     4621 cgccatttga ccttggacac tgcgatgatg ttctgcattg accatatcat ccatttctgc
     4681 tggagaaggt tcatatgttt catccgcttg gaattctcca ttcaatgcac ttaaaaagcc
     4741 ctcataaaca aacagcagtc cttgccctcc actgtctacg acaccgactt cttttaggac
     4801 tggaagcaag tctggagttt tagctaaagc acgctttgct ccttttacaa cagctgtcat
     4861 tacttcaacg acatcatcag tactttttgc tttcttttct ccagctttcg ctgcttctct
     4921 agctactgtc aaaatcgttc cttctactgg tttcattact gctttatacg cagtttcaac
     4981 tccatgagtg aaggctgcag caagatcaga agcatttaat gagacaacat ctggaatttg
     5041 cttagaaaat ccacgaaaca attgagacaa aataacgccg gaatttccgc gcgcacccat
     5101 cagcaatcct tttgaaagca ttgctgctaa ttctcctact ttttctgagg ttgaatctac
     5161 tactgcctta gctccactag tcatagatag attcatattt gttcccgtat cgccatctgg
     5221 cacagggaat acatttaatg aattgacgta ttctgcattt acttgcagac gattcgcacc
     5281 tgcctggacc atttcctgga actggcttgc acttatttct gttacactca cctagataat
     5341 cctccttcag atcgactgct gagttaccag ccagtctcct gcctagtctg gcagtacacg
     5401 aacaccttgt acaaagacat taactgaatt ggcagttacg cctaataatg cctctagatt
     5461 gtatttcact ttttcttgga cgttgcggga aacttcagag attttcgtac catagctgat
     5521 aatcgtataa acatctacag caataccatt atcttcttgg cggacaacta ctcctcttga
     5581 gtaattttct ttacgcaaaa tctcagtaat attatcttta atttgatttt tgctggccat
     5641 tcctacaaca ccgaacacat ctgtcgcggc gccaccaaca actgttgcaa ttacatcatt
     5701 ggtaatttca atggttccag ctgacgtttg aatttttaca gccatgaatg agcctcctta
     5761 acgggtaaaa aactcccatt tttcttctat catattttat catagcgcaa cttgaaataa
     5821 aaggcagtta cccaaagtac tcaaaaacct tgcaaaattt caggtataac cttgtttaat
     5881 ttacaattat tcttctcaat tgtcaattgt atctatgaaa atgtacttgc aaattcccat
     5941 tctatatgat atgttagtct ggtatgagct gaattattct attgctccaa atcaaggcaa
     6001 aggaggaaat taccatggca aaagtttgtt actttacagg tcgcaagaca aaaagtggaa
     6061 acaaccgttc acacgctatg aactcaacaa aacgtactgt taaacctaac ttacaaaaag
     6121 ttcgcgttat ggtcgacgga aaacctaaaa aagtttgggt gtcaactcgt gctttgaaat
     6181 caggaaaagt tgaacgcgtt taattgaatg atacttggac cgttcagata gtgaacggtt
     6241 ttttatttaa taaaaatttt taagggtggg acttttgatt cctacccttt ttttgcgttt
     6301 atttttgtta tacgaacaaa ttatgaagtt atttattcta aattttattt ttttgtgaaa
     6361 actagactct ttttaagcag agaaaaacaa ggatatgata aactgaatac aacttcatag
     6421 aaatttggtg gaaaataaat atgaaaaaaa atttaaagtc attctttaga gaaaacggac
     6481 gctacgcagc agctagtttc ctgattccgt ttttgatttt agcacttgtt tatctaagta
     6541 tcggaatcta tcctggaagc agcagaagta ttttggcaag tgatgccttt tcacagtttt
     6601 ctaattttca tgccagcttc cgaaatgttc ttcttggaaa gcaaagtatt ttttatacat
     6661 ggaatgcttc tctaggattg aattatttat ctttaatttc ttactatcta ggcgggatct
     6721 tcacgccact ggtgttcttt tttccaaacc agatgatgcc agatgcactg tatgttctga
     6781 ctttgcttaa ggtcggctgt gccggattgg ctttttggtt tcttgcacgt acgttcaaga
     6841 ttcctcgctg ggggcatgtt gcacttagcg tcagttacgc attgatctca tttatcactg
     6901 cacattcgga gatcatcatg tggctggatg cattttttta tcttccatta gtgattttgg
     6961 ggattcatcg tgttatggat cttaaacggc cgaaactttt atttatcagt tatttactct
     7021 tgtttttatc cagtttttat atgggtttta tgattggcgt attttctttc ttatatttta
     7081 tcgcccgtct attgacaaac tggtcagaat ataaaaaaac gattgttcct tatggtatca
     7141 cttctttact ggcaggcggc gcttcaatga tcattgtatt gccagcagtg ctggatcttc
     7201 ggtcaaatgg cgaggcactg agtcagatca ctacgttgaa aacagaagcg acttcttatc
     7261 tggatctaat catgaaaaat atgattggtg tttatgacac gacaaaatac ggatcgattc
     7321 cgttcgttta cgcaggtctg ctaccattag tcctctgtct gttttatttt gccagtcgga
     7381 aagtaccgtt gaaaaataaa tttttgtacg gcagtctttt cgccatactt atcgcaagtt
     7441 tttatcttgt tccattgaat ttgttctggc atggtatgca tgcaccgaat atgtttttat
     7501 tccgttatgc ctatctgttc tctttcttag tgctcctgct tgcaggaaaa ggatgggaaa
     7561 agatagaaca agacgatcgt gggctgttgt taggaatcat tttggtcgta gctgctctct
     7621 ttgcggtggc atggggcatc aaacctgaag gaagctacac ctatgtgacg atgacttcct
     7681 ttgtattgac agtggttttc ttagggattt atgcttttgc tgtgggattt tatcagcata
     7741 aaaaactttc attcaatcat ctgagtatat tgttattatt gatcatgtct gctgaagcac
     7801 tggtcaatac gaacagtatg atccatggca ttcttgatga ttggaattat gcttcccgca
     7861 gtttatatac tgatccttat cctgacttga aagctcttgt aaatgaaaca aaaaaagatt
     7921 ccgactcgtt ttaccgcttg gaaaatctgg atccagtatc tgcaaacgat tcgatcaact
     7981 atggttacag tggtatcagt ttgttttctt ctataagaaa tcgtaattct tcctcttacc
     8041 tggataaatt aggtttccgt tcaagaggga caaatttgaa tatccgttat aacaataata
     8101 ctttgctgat ggatgggttt acaggaatca aatataacat ttctaaaaac gaaagcacgt
     8161 tcagtaaata tggcttccgc caagctggtc aaagcggcga ctatacactg tatcagaatg
     8221 tcaatgcatt gccactagca tttactgcac cgttgtccgt taatgatgtt gagcagccag
     8281 tgacagataa tttgaccagt cagacaaact tgatcaatac attgtctggt ctaaatgaac
     8341 agtattttac attctataca cctactttga agaatcagtt gaatacgacg atcacaaaga
     8401 caacgactgg cgtcacttac tcagaaacag cagataacca gccaaaagag ctcacttggg
     8461 aagtccatgt gcctgcaaat acacaagctt acttaagttt gttcccaacc aattttgcgc
     8521 aattagaaag ttctagtgcg acgatcactg tcaatggtac atcgcgaaaa acgcaaatca
     8581 acatttctgg tcaatactat gatcttggct attatcctca agagacaaca gtgacattta
     8641 ctgcaagttt ttatgggacg aaagaaataa gtttcatgga gcctaaagtc gttggtctag
     8701 atgttgtagc ctatcaagcc gcaatgaatc agatcaaaga aaaaggtgtc gaaatggaag
     8761 ccactggcag aaaagcttct gggactgtaa ataccgctga agattccatg ttggtgacaa
     8821 ctattcctta tgacaaagga tggaaagcta aaattgatgg gaaaccagtc aaggtcagca
     8881 atttcaaaga cgcattcttg atgataaaag ttccagcagg taaacatact gtcactttca
     8941 gttacttgcc tgaaggattt acgataggtg caatcctttt tgtcctatgt attgcattgt
     9001 ttattcttta tgtctggtac cttgaccgca atcgcccctt ttatggcacg attgatccag
     9061 caatagaaga acaaccacga aacacacaca gaagaagacg tagaaaataa agaaacgatg
     9121 gaacctgttc agactgtccg caccaagggc aatcaaaaca acgttccatc gttttttggt
     9181 tacagaaaaa ataggtaaca aaaagatgat tcacttatca acgcctcgtc cacaagtcac
     9241 gatattcatc aaagatgaga agctgttttt tgaaattgct ctttgtgact cagagttaaa
     9301 tgacaggctc acgacctctt tcttaaacgg tgttcaatca gctgaccttc ggtattagct
     9361 caacaaacgg caaaatatga ggagctattt tcccgtttgt ttctctaata ctcaggtcaa
     9421 aacgctgatt tcacgacctc ttccttaaac ggtgttccag aagtagcttc ttcgaaaata
     9481 agccgaaatt ttgcaaaatt ttgagaagta attttcgtaa ataccttctt atttcttgaa
     9541 gctgaccact tctatcataa cctcttattc atatttcact tcatacaaat aaagtccttc
     9601 agggtgtgct gtaggtcctg ctgcattacg atcttttttc gcaataatat ctgggatact
     9661 atcttcaggc aatcggccat tgccaatttt caaaagcgtt cctactaaga tccgtatcat
     9721 cttatacaag aagccatttc ccctaaatgt aaacaacaat tcgtcttttg tttcatttac
     9781 ctccatctta gcctcgtaga tcgtacgtat tttatcctct atcgagcttc ctgatgcaca
     9841 aaaagaggtg aaatcgtgag tccctaataa atcaggaagc gcccgctgaa ttttttccaa
     9901 atcaagtggg taaggaaaat aacttgcata atggcgtcga aaaggactgc gtggtttccc
     9961 tatatccact cgaaattgat acgtcttttc tttgaccaaa tatcttgcgt ggaagtcttc
    10021 tgaaacaatt tctacttgtc ttacagcaat atcctctggt gactgggtat ctagcgcgaa
    10081 acgcattcgt tctaacggac gctcctctgg ataatcaaaa tggattactt gcccgatcgc
    10141 atgtactccg gcatctgtcc ttcccgaacc aaagaccgtg atttcttttc catttgccat
    10201 tttttgtaat gttttttcta cttcttcttg aacagtacgc ccatttggct gtttttggaa
    10261 tccattaaaa tttgttccgt cgtacgcaat gattgctttg taacgtacca tgtttttctc
    10321 tcctttgttc ctaaaaggtt atctggaata atgatttttc tataa
//
LOCUS       DANKAY010000095        10191 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000095 DANKAY010000000
VERSION     DANKAY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            106..255
                     /locus_tag="PWY98_002314"
     CDS             106..255
                     /locus_tag="PWY98_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2038032.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            266..1780
                     /gene="dltA"
                     /locus_tag="PWY98_002315"
     CDS             266..1780
                     /gene="dltA"
                     /locus_tag="PWY98_002315"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2038033.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1777..2982
                     /gene="dltB"
                     /locus_tag="PWY98_002316"
     CDS             1777..2982
                     /gene="dltB"
                     /locus_tag="PWY98_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2038034.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3027..3260
                     /gene="dltC"
                     /locus_tag="PWY98_002317"
     CDS             3027..3260
                     /gene="dltC"
                     /locus_tag="PWY98_002317"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2038035.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3263..4531
                     /gene="dltD"
                     /locus_tag="PWY98_002318"
     CDS             3263..4531
                     /gene="dltD"
                     /locus_tag="PWY98_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2038036.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4672..5754
                     /locus_tag="PWY98_002319"
     CDS             4672..5754
                     /locus_tag="PWY98_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2038037.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5754..6557
                     /locus_tag="PWY98_002320"
     CDS             5754..6557
                     /locus_tag="PWY98_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2038038.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6627..7367
                     /locus_tag="PWY98_002321"
     CDS             6627..7367
                     /locus_tag="PWY98_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2038039.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7378..8382
                     /locus_tag="PWY98_002322"
     CDS             7378..8382
                     /locus_tag="PWY98_002322"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2038040.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8427..10124)
                     /locus_tag="PWY98_002323"
     CDS             complement(8427..10124)
                     /locus_tag="PWY98_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038041.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3266 a         1693 c         2070 g         3162 t
ORIGIN      
        1 gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc
       61 aaaattagtt acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt
      121 tctaatccaa aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca
      181 gtactgctgt tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc
      241 tataacgaat tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga
      301 ttggggaata aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg
      361 tgaattgaaa caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga
      421 tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt
      481 tgtcaaatct ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct
      541 atcgatcttt cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga
      601 tattccttcg gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt
      661 aggattttat cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc
      721 tggtactact ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt
      781 tcaatggacg atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc
      841 attttctttt gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct
      901 gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc
      961 cttgaagttg aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc
     1021 tacattcaat ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt
     1081 gacgaaaaaa actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac
     1141 ttacggtcca acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact
     1201 agatagttat gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt
     1261 ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag
     1321 cgtatcaaaa ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa
     1381 tgacttagca agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt
     1441 ttatgaagga cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga
     1501 tatcgaacat tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata
     1561 ccaaggaaca aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca
     1621 gccggatttt cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta
     1681 tatgattcct cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat
     1741 cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct
     1801 tatgcagagc cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct
     1861 ttattgatat gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta
     1921 tttgtcagct ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg
     1981 tggcagacga tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga
     2041 tggttttttg ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct
     2101 ttttttgaac atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt
     2161 aaatctgtac aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt
     2221 cgttatatcc agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat
     2281 aggagatttg aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg
     2341 ggaaaaggcg tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat
     2401 tttggtcaag tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca
     2461 tgggcattag taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc
     2521 tatagcttat ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc
     2581 aataaacctt ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg
     2641 tccttttggt tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa
     2701 ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg
     2761 ggtatatggc atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg
     2821 atctgtctga cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct
     2881 aacaaattta cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc
     2941 ctgatttttt caggattttt agataaactt ttttttagct aacgaatagt gacatattaa
     3001 tttatataac aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa
     3061 taacaggaac agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc
     3121 ttgattcttt agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag
     3181 tccctgtatc tgaattcgag cgagaggatt ggtgcacacc gaataaaatc attcaacaag
     3241 caactgcatt gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt
     3301 tttagctgtt ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa
     3361 tccagaaacg attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc
     3421 gataaaaaat tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt
     3481 aagcagaatc agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg
     3541 tccatttttg ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc
     3601 tgcaggagat gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt
     3661 taaaaatggt gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga
     3721 ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt
     3781 aactttctca aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa
     3841 agggaagcta cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa
     3901 gagagaggat gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca
     3961 tgagtcaaaa cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca
     4021 aattggagaa cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca
     4081 tcgtatccga gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt
     4141 ttcgccagaa ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga
     4201 agtattgttc atcatcccac cggttaacga gaaatggagc gattataccg gactatctca
     4261 agaaatgtta caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa
     4321 tcgaatagct gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca
     4381 tctaggctgg aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa
     4441 tcatattact gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca
     4501 tcaaatacca gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt
     4561 ttctttattt ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa
     4621 tcataagcaa tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca
     4681 aaagaagaac agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa
     4741 gaggtaagag aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac
     4801 ggtttaggca gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct
     4861 ggacatatgg acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa
     4921 ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa
     4981 acacgtgaag ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca
     5041 cctcaagtaa gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca
     5101 agccaagaag aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt
     5161 gaatacaaac gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc
     5221 gggacagctg tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt
     5281 ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca
     5341 caccttgtaa atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca
     5401 ggaatgacac caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt
     5461 gatgcatcta tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa
     5521 atggatattc cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg
     5581 catttgacaa gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat
     5641 tcccatacat cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa
     5701 ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata
     5761 atagaatacc tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg
     5821 gaatcaatac ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt
     5881 ttgacggttt cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag
     5941 tcgataattt acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg
     6001 tacggggtgg cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg
     6061 gttcgatact tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa
     6121 tctttctgcc catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt
     6181 caaatgcggt acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat
     6241 cgatgatagg gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc
     6301 aatttgctgc atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat
     6361 atggctggtt tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta
     6421 tcggctggta tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt
     6481 atgtgaatgt gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag
     6541 acgcctattt tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag
     6601 gataaatctt tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt
     6661 atccttataa aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt
     6721 tgatcgatgc aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc
     6781 tagatgctgt gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac
     6841 agtattactg gcaacttcat cctaagagaa atccggagac attgctttca atttatggac
     6901 acacaaaaga ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc
     6961 cttattttga agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa
     7021 tccatccagt agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg
     7081 tatttacagg tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt
     7141 tgtttctagc agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt
     7201 caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc
     7261 tacctcagtt tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag
     7321 ggattgatat agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg
     7381 atttttgtac caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg
     7441 ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc
     7501 atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat
     7561 ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac
     7621 ctgaacttta gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta
     7681 acacagccaa tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc
     7741 cgaaatgatc tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac
     7801 ggtcggatgt ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat
     7861 gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt
     7921 gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt
     7981 cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac
     8041 gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat
     8101 tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct
     8161 atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc
     8221 tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag
     8281 aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt
     8341 ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa
     8401 tccacatata tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga
     8461 acagtgatcc gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc
     8521 gctacgtctt tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg
     8581 cctcgactaa ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt
     8641 tcctcttgtt cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga
     8701 gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg
     8761 ataggaagtt cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct
     8821 aacttctggt aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc
     8881 atttcgaaat tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg
     8941 tccgtatgca ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta
     9001 tttacttctt ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg
     9061 atttttaaga aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga
     9121 ttgtagctga taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt
     9181 gtttgataga tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag
     9241 acgatccaag gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat
     9301 gcaagtgaag tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca
     9361 ggaagttctg caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg
     9421 aacgtacaaa aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag
     9481 atgatgaaag ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt
     9541 tcaaaaagaa taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca
     9601 aagcaactta agagaataag tgagtattca agacgttttt gtttatacat caagtagatc
     9661 gtatagaaaa aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc
     9721 actgattgtg aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt
     9781 ggcagtccag catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata
     9841 taatttgctg gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa
     9901 tctttccgtt ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct
     9961 ttgattagta aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt
    10021 tcgtctttat ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga
    10081 tcatcttttg tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca
    10141 ggagaaaaaa taaaataaaa gaaataagca attcctgtta aaaataacaa t
//
LOCUS       DANKAY010000096        10186 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000096 DANKAY010000000
VERSION     DANKAY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            47..2743
                     /locus_tag="PWY98_002324"
     CDS             47..2743
                     /locus_tag="PWY98_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2038042.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2820..3308
                     /locus_tag="PWY98_002325"
     CDS             2820..3308
                     /locus_tag="PWY98_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2038043.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3320..3814
                     /locus_tag="PWY98_002326"
     CDS             3320..3814
                     /locus_tag="PWY98_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2038044.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3835..4644
                     /locus_tag="PWY98_002327"
     CDS             3835..4644
                     /locus_tag="PWY98_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2038045.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4641..5462
                     /locus_tag="PWY98_002328"
     CDS             4641..5462
                     /locus_tag="PWY98_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2038046.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5513..7612
                     /locus_tag="PWY98_002329"
     CDS             5513..7612
                     /locus_tag="PWY98_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2038047.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7609..8727
                     /locus_tag="PWY98_002330"
     CDS             7609..8727
                     /locus_tag="PWY98_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2038048.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8766..9644
                     /locus_tag="PWY98_002331"
     CDS             8766..9644
                     /locus_tag="PWY98_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2038049.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9835..9987
                     /locus_tag="PWY98_002332"
     CDS             9835..9987
                     /locus_tag="PWY98_002332"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2038050.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3125 a         1674 c         2280 g         3107 t
ORIGIN      
        1 agatatctga ggcaaatatt cgactatttg tgtttcaaag taaaaattgg aatacaaata
       61 tagaatagac atattgcagc cgatagttta ttatgctttt agacacatta aactggagga
      121 aaaaatgaaa aagagcgcta aagatcgagt ttttgatgag ctgatacgac taacagggaa
      181 tatgtcttct gtcactgccg agaaaatttc gaagaatcta ggtatctctc gtcagaatgc
      241 cagccattat ttgacgagat tagtggaaga taaaaaggtc gagaaattac cgggaaagcc
      301 tgttttgtgg aagcctttag atgaatatgc ggttgtagat aatacggagc aaataaacga
      361 agcctttcat tctgtagttg gtcatgacgg aagtctgaga gaggtcattc aaaaatgtat
      421 agccgctgtc aaatatccgc ctaacggact cagtgtatta ataaatggag ccacgggtgt
      481 tggaaaaagt tttttagcca caaaaatttt tgaatacgct atccatgagc aaattattga
      541 aaaagatgca ccatttgcta tcttaaactg tgccgattat gcggacaatc ccgaactgct
      601 ttcggcaaca ctgtttggat acaagaaggg ggcctttacg ggggctgaaa aggataccga
      661 tggtttattg gcaacggcca acaacggcta tctttttctc gatgaggtcc atcgcctatc
      721 gaaagaaaat caagaaaagc tctttctatt tattgatact ggaaactatc gtccagtcgg
      781 tgaaaatgta aattggcatt ctgctaaggt tcgttttatt ttcgccacga cggaaaaagg
      841 tgaaaattat ttattagaca cctttgatcg gcgtattcaa attagtgtta tgttgccaac
      901 ttttgaagat cgccccattc gagaacgatt agagctgatt caattatttt ttcaaaatga
      961 agcggatgtt ttacaaaaag atattattgt ttcaagagaa gccttgtctt taatgttatc
     1021 tcatcccttt agtgggaata ttggtaagct gaagaatatc attaaaatca gttgtgcgga
     1081 tgtgtatagt cgaacacaag aagaaccatt aacgattggg aaaaatgaga tcttgattca
     1141 gttaaatatg ctggaatccg aagtagaaag cttgccgatt gctagtttat tgataaagaa
     1201 aaatcaaaag ataaaaacgg atcgaatcaa accgtttgat tatgattcga ttttggaaaa
     1261 aattactcag ctgataaatg aaggtaattt caatgaaaat ttttcttcga taaagaatga
     1321 agtacagcat ttggtgcatc acattgataa ggattctttt ttatcaatga gtagaaaatt
     1381 gatgattcag atgtttagaa aggtctgggt atccgtttta ggaaaaacgt acggattgac
     1441 gacgagtgga cctgttgcag aaattattag cagatcctat gtcatttccc caagctataa
     1501 gacagactta accgggataa atcgtacatt agctgcaaat ctacctcgga cgacttactt
     1561 atgtaatcag ttcttggagc atttaccacc gctctcagaa aaggacgaga actttctaag
     1621 actgcttatt gtcgtgactt tatctgatta tgtagatgaa tccatcgagc tgaagggcct
     1681 gttgcttgct cacggagata gtacggctag cagcattcaa gcagtagtga atcaattatg
     1741 cggcaattat gtttttgaag cgttggatat gccagtagag agtaatgtgt cagagattgt
     1801 cgaatcgaca aggaatttcg tgaaacggca agtgaataca gatagtctag tgctgatcgt
     1861 agatatgggc tctttaaatc aaatttacac acagatcaag aacgatttaa aaggggagtt
     1921 acttcttctg aataatttga cgacttccat tgcacttgat attggcttga agatgacgat
     1981 gaatgcgcca tttaaacaga ttgcggagga tgcagcagac aagtattcaa tcaatgttca
     2041 atactttgaa ggcttttctc aaagtagcaa cattatcatt tcctgtatgt cgggtttggg
     2101 gatttcggac aaattgaaag atgtttttcg tcatcattta tcggatgaat ccatcgaagt
     2161 tttcacgaag gactacagag aactacgtga attaatcgac caaaatgatg aaacttattt
     2221 tgacaagaca aaattggtca tcacgacttc ggatcttcca cgttcctttt caattcctaa
     2281 tattaatgtt tatgacatct tagacggtga tggagcagcc aatttggagc atattttgag
     2341 tcctgaaatg aagccggcag aatttaaccg cttgattcag gagctagtaa aattcttttc
     2401 gttggaagga attgcggacc gtttgaactt tctgaaccca acgatcgtga tgagtgaagt
     2461 ggaaacagtc atttctaaat atgaaaatta ctatcatttc actttaaatg gccgtgtaaa
     2521 actaaatctc tatatgcata cggcattgat gatggaacgt ttattcttgg ctcggacaga
     2581 aaaatctgaa gaaccgaatt atgatttgaa accagatgaa gatgaatttt attatgtttc
     2641 tagaagtatt tttcaatcaa tggagatgaa atataatttt aaagtcaatg cttatgagct
     2701 ttcgctgctc tatgaattat taggaccgta tattcaaaaa taaagtgtct aaaaaagtgt
     2761 ctaatctgtt ttttttaggt tagacacttt ttttaatgtg aattaacgtg attaaaccaa
     2821 tgtttttaaa agttggcaca ggaattgctt atataaaagt gaacagaaag aaaggaatta
     2881 accatatgaa gaaaattcta atagcgactc acggtcactt agcttcaggc tttcttagca
     2941 gtattcaatt actgacgggg aaggttcaag agattacggt gattaatgct tatgtggatg
     3001 agtgtgattt tgaacaggag ttgaatgtat ttgtaaacca ggtgaacgag caggatcaga
     3061 tttttgtttt taccgattta tttggcggaa gtgtcaatca gaagattacc aaaacctttt
     3121 tggagaaaga gattgctgta actattttgt ctggctttaa tctgccaatt ctgctcgaaa
     3181 tagtattggc aaccaaggaa ttaacaaagg aggatattca ggaactggta aataaatgcc
     3241 aacaggaatt aaagatgacc attcttgatg aggtacatgt agtaaatgaa gaggaagatt
     3301 ttttttagga ggaagaaaaa tgattaaagc agtgcgaatt gatgaacgat taattcatgg
     3361 acaagtagcg atgacttgga caaagagtct aaatttaacc gggttagtag tagcaagcga
     3421 tgaggcggct accaacgata ttcaaaagat gacattgaag atggctgcac catcgaatgt
     3481 caaagtcatt attaaaacag ttgatgacgc gattcatttg ttaaaagatc cgcgggcgaa
     3541 ggacatgcgc ttgatggttt tagtccctaa tgtcaaagat gcggtaaaag ttgcgaaagc
     3601 tttcccaacg gaaatcgaat taatgaacgt ggggaatgct gggaaaatga caccgccatc
     3661 ggaagataag aaaaccttat caaaggaagt gatgttaacg acgtcagaaa ttcaagactt
     3721 aaaagaatta gttgaacttt atccagatac cgtttttcag cctacaccgg cgatggaaaa
     3781 acgttcagct atctctattt tgagtgattt ttagaaaaag gaaggagaaa aattatgtat
     3841 gatgcatttg tagtcgcttt ggccgttttt attggagttg ctggacatga aatgttcggg
     3901 atgtccatgc tgagtcgtcc gattgttgtt gctccgttag caggagcgtt gatgggggat
     3961 ttgcagacgg ggttgattgt cggggcgtca ctagaagcca tctttatggg tgtcgtcaat
     4021 atcggaatct ctagtacggc ggagccttct ttggctgctg ggctggctac agcgttcgca
     4081 atcaaaatgg gaggaaacgt ggatgcgatt attcccattg tctttccact agcagtatta
     4141 ggattacagt taatgaatat gattttttct tttgtatgtg gaccgatggc tgctaaattt
     4201 ttcaaatttg caaaatctgg tgatcaaaaa ggaatcatca atctgcactt ctttatgtgg
     4261 tttgtccact atgcgcttta tgctcttatt ccattctttg ccgttttatt tggagcagat
     4321 gttgtcaaaa gcgtcttgga taatattcca gaggtcatca tgaacggatt aacagtcgct
     4381 ggaaatctgc tgccggcagt tgggatggcg atgctgttac ggatgctttg gagtaaaaat
     4441 attgctgtgt ggtttttctt tggggccgtt gttatggcgt atctcgagtt accattgatt
     4501 gcaattgccg cagttggaac gattattgca attgttatag cccaaagaga tctacaacta
     4561 aaaaatatat ctgttcatgc agttcaaagc gatgctgctg taaatgcaac gcttgaccaa
     4621 gaagaggagg atttctttca atgaaattag ctgatggaat ttctaaagat gagaaaaaat
     4681 tggttcggaa gatgttttgg cgctcgatga ctatgtatgc ttcggtgaat ccggtgacga
     4741 tgggcggcgg tggtttcgcc tattctatga tgcccttcat taatcatttc tacaaaagtg
     4801 aagaagatcg agctgcggcg ctggaacgtc atacacagta tttttcgacg accattcctt
     4861 gcgcgtcctt tgtcatgggg attgcggcat cgatggagaa ggaaaattct gaaaaagctg
     4921 gttttgacgt ggaaagtatc aacgcgatca aaacgagttt gatgggacca ttggctggaa
     4981 ttggcgattc attgttctgg ggagtttggc gtgtcgttac cgcgggacta gcgatcaact
     5041 tagccaatgc cggaaatatt ttggcaccaa ttatcttttt agttatgttc aatataccta
     5101 attacttgat gcgatactat ggcggcgttt taggctattc attaggctct aaatatattg
     5161 aaaagctgta tgcgaacggg ttaattgaaa ttctgacaaa agctgctagt atcttgggtc
     5221 tgatcatggt aggtgcaatg acgatcagta tggtcaaatt caataccgtt ttgagctttt
     5281 caatgaaggg cgaagaacta atgaatgtcc aagaaatcct cgatagtatt tttaaaggaa
     5341 ttgtaccgtt attggtaaca ttgagctgtt tcaaattact agataaaaaa gtcggcgtca
     5401 tcaaaattat ttttggaatg gtcgtcttgg gaattttatt gtcattactt ggaatcgcat
     5461 agtaggaagc tgcagctact aggctgcagt ttttttagca aggagggaaa agatgactat
     5521 taatgaagta atttattcac ccaaacaccc attttttaat gctgagttaa acgacggaaa
     5581 tgtgactact tcaaacgaat acagctacta tcggaatatt cctgatgtaa agggattgac
     5641 cttcgatgta agtaatcaaa agacattagc aaaccttgat gtttgcggga cgattcaaac
     5701 gttgaccttt tatcgagaaa atttactaac tgaggaaaaa ccaggtgttt gggtcaataa
     5761 acagctctct caaaccaatt cgttatcctt aaaggttgaa gtaaacggga ttgtctacca
     5821 tttgtcagaa tcggatcatc gtattgaagt cgatgtgatt caagactgtt tgcctagata
     5881 tattcatcat tattcgacgt ttaaggtcat tgtagtatcc tttgctccca tactggctaa
     5941 taagcgttta agtatgcttg ttcaacaggt ttacattgtg aatgaaacac acgaaagcct
     6001 tcaagtttca gtgaaagatg tgccttcgta tcaagagaag tattcggatc agcaaaatgt
     6061 gttgatttct caaaaaggaa ataaggacac gatcgcacaa caggaagtgg caagcttcgc
     6121 cattgctttt gtggacccaa atgcttttga agaagtaatg acttttcagg aatctgatat
     6181 cgaaaaatgg ctgaaggaaa cattgaacta ttttgctcag ctatttgggc agctgtcaat
     6241 gccaaacgat agagttgtcc atctctatcg tcgtgcactt tatcaaagct tttctagttt
     6301 cggtatgaat cgacagggac aggtcgtcgg cagcaattgg ggcagctatc ctgctactaa
     6361 tcgaatttgg aataaggata tgtattacgc atcattgcca ttcaccatgt ttgaaccgga
     6421 gttatgtcaa aaaacggttc tatggtttga tcaatatggc gttaagtttc ctggttcaaa
     6481 atttacagga ggtatcaatc attctctgag taattcgtta tccagtgcgt tactggctag
     6541 tctttatttt gaacatactg gggatacaaa attctttgag cggtgtccgc aggttctact
     6601 gaatgcttca agaattattg aggacatatt agctcaacgt cggtcagggg aaccaatgct
     6661 ttttccatct gtttggttat cagacgcctt tgcacttgga aagtatcata ccggcagcaa
     6721 tctttgtctt tggaaggcct gtattggact tgcagaaatt tttgaagccc tagaacaact
     6781 gagtcttagc aagcgataca aaaacattgc ctgtaaacta aaggaatcaa tttgtaaaca
     6841 aatgactatc gatggtcctt ttggtgagca atttttggag ggaatcggtg atgaggagaa
     6901 aacaacctat tcagtgaagc actatcaaaa accgattctt gagcaagggt taatcttttt
     6961 aagtgatgtg attgtggatg gaaaaattaa tttattgatg catgatggag aagaatcaga
     7021 cactacgttg atgccttttt atcaattttt agataataaa gatcatttat atcagaacac
     7081 catggccttt tctgctagtt ctttcaatcc aacctatagc gaggaaatca aaggcatcac
     7141 ttggggatta gaatccggcg ccacttttcc tggctttatc actgttttaa tgagcgagct
     7201 gcacaaccct caagctttcg taacccgatt agaagaacta atttgtttgg cagatttaga
     7261 cggttcgtgg tggtggtggc cgtatcgctt agaggctcaa caaggagaag tcgtgcgaga
     7321 ctttggctgc ggcaaatgcg gctgggcatc gggtcttttt gtcagcctaa tgatcagtca
     7381 atatttcgga ttgaaattca aaaaaggagt attgcaaatt gatcctgttg ataatttaac
     7441 gttttcttgg aagaatttga agttcggtca agccttcatt tcgattgaat gtacgcaatc
     7501 cgagttgagt atttcaaatt tgtcagaaaa gccattgaaa ataagtgata cgaaaaaaat
     7561 tcttcacgta gaaaagggaa aaacgattca cattcaaagg agaaaaagat gagtatacta
     7621 aaaaatatta cccaatttaa aaaaatccag agtcgagcaa tcactcccga aaatccaaca
     7681 ggcgagaaag gtaaagctgc gatgttgcca agcgagctag gaccaagccg caaaggtcgt
     7741 ggcgcaattc cgctgacttc tggtgaagaa actgtgattg cggatattac tggaacggga
     7801 ataattcgtc atatgtggat gacgattcgt gatcgaacag aatttggcag ttttgtttta
     7861 cgtgatttaa tcttaagaat ttattgggat gggtcggata gccctgctgt tgaaagtcct
     7921 ttaggggatt tcttttgtaa tgggtttgga gagcgttgcg aggtgaactc gttgccgata
     7981 gtggttaatc ctacaggggg ctttaattcc tatttcgaaa tgcctttcca aacatccgct
     8041 aaaattacga tcgagaattt acatccgaag gatatgtcct cattttttta tacgataaat
     8101 tacgctttgg tggacgaatt ggaagaaaat agcctatatt ttcacgcaaa ttggcatcgg
     8161 gaacgcatta ccaaattaca ggaggattat aagttaattg atcgattaaa gggtcaaggc
     8221 tattacgtgg gtacttacct tgcgcttacc tgcctagaac gttattggtg gggcgaaggc
     8281 gagtttaaat tctacattga tggggatagt gagtatccga ccgtaacctc aacaggttca
     8341 gaagattatt ttggcggggc gtgggccttt catgagtacg atgagttcgg acgtccacat
     8401 gcgaaaaact attcaacagc ctttttaggg tatccttacc aatcaaatag agatgccaca
     8461 agagatcgtt ttgaaacggg aaaattgaat gccgttcatg cctttggtga cgacgcaatg
     8521 ccacgacatg gtctttatcg ttggcatctt caagacccca tctgttttac cgaagattta
     8581 tcggtcactt tacaacaaat cggcaacgat gatgttcgct tatttgagcg gcaagacgat
     8641 gttgctagtg tcgcttactg gtattcaaca gaagcttcag gaaatactaa acgtcatttc
     8701 actgatcgag aacgcttgcc gcgttaatga agtgtttttc acatattcga agggaggaac
     8761 ctatgatgat atatggggca attgaagctg gagggactaa atttgtctgt gcagtcggta
     8821 ctgaggaatt aacgattttg gaaagagtca gttttcccac aacaactcca gttgaaacaa
     8881 tgaagcaggt cattgatttt tttaaacctt ttaaggaaca attggcaggc atcggggtcg
     8941 gctgctttgg tccaattgat attcaatgca aatcaacgag tttcggtcat atcacatcaa
     9001 caccaaaact tgcttggcaa aatttcaatt ttgtaggaac gctaaagcaa tatttcaata
     9061 ttccgattga atggacaaca gatgtcaatg cggcttgcta tggtgaatat gttgctggtt
     9121 ctggcaaagg gttagcaggt gttgcatact tcactgtggg aacaggtatc ggaggtggtg
     9181 ccttgttcaa tgggactttc gtggaagggt tcagtcatcc ggaaatgggg catatgttga
     9241 tcaagcgaca tccaaaggac aatttttcag gaaactgtcc cttccaccaa gattgtttag
     9301 aggggatggc agcaggacca gcgattgaaa aacggttggg tgtaaaaggc cagaatttat
     9361 tggctgacga ttcgttttgg cagattgaag ccttttacct tgcccaatgt gcatacaata
     9421 caacactgat gttttcgcca gatagaatta tttttggcgg tggtgtaatg aaacaagaac
     9481 acatgaagaa aaaagttcag gataaatttg ttgagcttat aaatggatat gttgaaatac
     9541 caccaattga tagctacatt attacgcccg aattaggtga taatgccgga ataatcggcg
     9601 gattagcatt ggccagaaaa gcggtaagaa ataagcaacc ataattaatg aatgtttcat
     9661 tgatactcat gtcaaaagta tttaaccgag taatcaaatg atcaaatgaa gttggataaa
     9721 gcgaaaatga attcaaataa aaaggctcta agtcaaatcg tgttgttttc attaaatcac
     9781 tgaaaggaaa tgcttctgcg tttccttttt tctttttgtt gtgcgctgta taccttgagg
     9841 atattcactg gtggcgtaca cttaaatagt aatatattgt gttactttat gtctgtggta
     9901 atagttatag ttgcttacca atgtatgttc atttttcgaa atgttgactt cataatcctt
     9961 ggcttaatgt ttgaattttt ttggtgatag attttttcag ccgtataaaa taaatcgtaa
    10021 ttctattata gagagttgtg attataaaaa gatagcaaga aaaaaaaaga aagtttctat
    10081 acattttaaa tattttattc ctatttctat tgctaaaaaa gtagaaattc tatatttacc
    10141 tttaactctc gcttctcttg caaatgtagt tagcatttta atcgat
//
LOCUS       DANKAY010000097         9870 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000097 DANKAY010000000
VERSION     DANKAY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            254..733
                     /locus_tag="PWY98_002333"
     CDS             254..733
                     /locus_tag="PWY98_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038051.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="PWY98_002334"
     CDS             766..2994
                     /locus_tag="PWY98_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2038052.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="PWY98_002335"
     CDS             3123..4766
                     /locus_tag="PWY98_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2038053.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="PWY98_002336"
     CDS             complement(4839..5384)
                     /locus_tag="PWY98_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2038054.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="PWY98_002337"
     CDS             complement(5407..5892)
                     /locus_tag="PWY98_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2038055.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="PWY98_002338"
     CDS             complement(5882..6694)
                     /locus_tag="PWY98_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2038056.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="PWY98_002339"
     CDS             complement(6833..8365)
                     /locus_tag="PWY98_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2038057.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="PWY98_002340"
     CDS             complement(8474..9505)
                     /locus_tag="PWY98_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2038058.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3289 a         1836 c         1900 g         2845 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa
//
LOCUS       DANKAY010000098         9811 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000098 DANKAY010000000
VERSION     DANKAY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(34..2154)
                     /locus_tag="PWY98_002341"
     CDS             complement(34..2154)
                     /locus_tag="PWY98_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2038059.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="PWY98_002342"
     CDS             2384..3562
                     /locus_tag="PWY98_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2038060.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="PWY98_002343"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="PWY98_002343"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2038061.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="PWY98_002344"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="PWY98_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2038062.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="PWY98_002345"
     CDS             complement(4915..6168)
                     /locus_tag="PWY98_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2038063.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="PWY98_002346"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="PWY98_002346"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2038064.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="PWY98_002347"
     CDS             7746..9092
                     /locus_tag="PWY98_002347"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2038065.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="PWY98_002348"
     CDS             complement(9400..9786)
                     /locus_tag="PWY98_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2038066.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DANKAY010000099         9700 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000099 DANKAY010000000
VERSION     DANKAY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            50..1423
                     /gene="rlmD"
                     /locus_tag="PWY98_002349"
     CDS             50..1423
                     /gene="rlmD"
                     /locus_tag="PWY98_002349"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2038067.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            1426..1554
                     /locus_tag="PWY98_002350"
     CDS             1426..1554
                     /locus_tag="PWY98_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2038068.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            1994..2182
                     /locus_tag="PWY98_002351"
     CDS             1994..2182
                     /locus_tag="PWY98_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2038069.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            2260..3273
                     /locus_tag="PWY98_002352"
     CDS             2260..3273
                     /locus_tag="PWY98_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2038070.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(3340..4374)
                     /locus_tag="PWY98_002353"
     CDS             complement(3340..4374)
                     /locus_tag="PWY98_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2038071.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            4489..6714
                     /locus_tag="PWY98_002354"
     CDS             4489..6714
                     /locus_tag="PWY98_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2038072.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            6732..8615
                     /locus_tag="PWY98_002355"
     CDS             6732..8615
                     /locus_tag="PWY98_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2038073.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            8596..9570
                     /locus_tag="PWY98_002356"
     CDS             8596..9570
                     /locus_tag="PWY98_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2038074.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         3219 a         1741 c         2034 g         2706 t
ORIGIN      
        1 ttatagtaga gttcagtagg attgaaaaat aggcttaaag gagcaaaata tgacagaaca
       61 gcaaatagta aagaaaaacg aacgatatac agtcgaaatc gtcgacttga gttatgaagg
      121 aatgggggta gcaaaaatcg atggttatcc attatttatc gaaaatgcac tccctggaga
      181 agaagtagaa atactagtag taaaagcagg caataagttt ggatatggaa aagtcgaaac
      241 atttattaca caatcacctg atcgtcagcc tgtagaaaac aatgcattac ttcgcatagg
      301 tatcgcacct ttagcccatt tgaagtatga acaacagctt gtcttcaaac aaaaacaagt
      361 agagcatgta atggcgaaga ttgctaagat gccagaagtt cctgtacttc caacggtcgg
      421 aatggcagaa ccattcggct accgtaataa agcacagatc cctgttcgcc gtatcgatgg
      481 acagttagcc accggttttt acaaaaagaa tagccatgat ctagtcccta tcgaagattt
      541 ttatatccaa gatcctgtca tcgatcaagc aatcaaagta gtcagagata tcttgcaacg
      601 gttccaagta cgaggatata atgaagaaaa aaatgaagga cagcttcgtc acatcattat
      661 ccgtcgtggt cattattccc atgaaatgat ggtcgtttta gttactcgta aagaaaaatt
      721 ctttaaatcc aaggaaattg ctgaagctat tcatgaagaa ttgccagaag tggtttcggt
      781 catccaaaat atcaatgaag aaaaaacaaa tgtgatttta ggagacaaag aaaaagtttt
      841 atatggcaga gactatatcg aagatcagct attaggaaaa acctatcgaa tctcagcaaa
      901 gtcattttat caagtaaata ctcaacaagc agagtatctg taccaaacag ccatcgagtt
      961 tgccgagtta gataaaaaag acattgtgat cgacgcttat tcaggaatag gtacgattgg
     1021 cttgtcttta gcagacagag tggataaggt gtatgggatg gaagttattc cagaagcaat
     1081 tgaagatgca cagttcaatg ctttaaataa taaaatcgaa aatgttcatt atgaagtcgg
     1141 aaaagcagaa aaagttatga aaaaatggca agaaaaaggg atcaaaccat ctgtgatcgt
     1201 agttgaccct ccacgaaaag gattagatca tcattttatt gaatcagcaa tcgaaatgtc
     1261 accagaacga atcgtctata tctcatgtaa tccggcaact tttgcaagag atgctaagtt
     1321 atttgcagaa gcaggttatg ccgtgaagaa agttcagcct gtagatcttt tcccgcaaac
     1381 ccatcatatc gaattagtcg cttcattcgt taaaggagaa taaaaatgga ccaaaaagaa
     1441 aaaatgaaag aattgatcaa aaagaaacaa ggtggaggcc gatccaaaca aatggaaaca
     1501 cctaagaatg atcgtaaaaa catgagaaaa ggccctaaaa tctttaataa atagacgtgc
     1561 taaagaaaga ctaagaagtg ttaacttttg ttattgtttc ttagtctttt tagttagtct
     1621 acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca aatctgggtc
     1681 agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa ctgattgatc
     1741 aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca tgtttgacgc
     1801 ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca gacccattat
     1861 tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg tgctgggagt
     1921 tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt tgactgtaac
     1981 gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg aagcgacacg
     2041 atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt caacagaaga
     2101 agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag cattgcagaa
     2161 gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata acactatggt
     2221 ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa aattcttaaa
     2281 tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt tcttgccttc
     2341 cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa tactgttcaa
     2401 atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa aacagatttg
     2461 acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa aaacagcaag
     2521 gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg agataaatcg
     2581 ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt ggttgcgatg
     2641 aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt gaatgaactg
     2701 atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga acaattattt
     2761 tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat acaaacgaat
     2821 gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag tctgaaagga
     2881 accatttctt attgcggacc tgttgatttg aagctaacag cacagcagca ttcggatgat
     2941 cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac aaaagattgg
     3001 aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa ggaatttccg
     3061 ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca agcattagaa
     3121 aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga tacaaaaaaa
     3181 gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa ttgttaccag
     3241 cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc gtttcgtttt
     3301 gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt ttgttcttgg
     3361 gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg gttcgtcccg
     3421 tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg atttcgcatt
     3481 gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat tatcaaagct
     3541 tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc gatagacacc
     3601 aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt cattttctaa
     3661 tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat gataagacat
     3721 atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc tcttttcatt
     3781 tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc cgatataggc
     3841 tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca tccgttcaag
     3901 atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga cgactatttg
     3961 catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa caacgatcac
     4021 cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat tatagattcg
     4081 attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca aatgtttgta
     4141 atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa tcgaaggcat
     4201 tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag ctgcctctac
     4261 tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca acacacggga
     4321 aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa ccatttttac
     4381 acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa cagtatacag
     4441 gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat ggatattacg
     4501 catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt cttccattta
     4561 cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt ggcgcatatt
     4621 tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg aagagatcgt
     4681 ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga tacactaccg
     4741 caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat cattcagcgc
     4801 aagaatggat cagacttact ggatctgaga tatgacagcc attatattac agatgggaag
     4861 ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc acaaacgttg
     4921 gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta taccatattt
     4981 actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg agaaacaatc
     5041 aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg atttgatgaa
     5101 gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca atctgtttta
     5161 tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat gaataacttt
     5221 attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg acttcaattt
     5281 gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca attgagagtc
     5341 gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca gtcttttcaa
     5401 acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag tcagatccac
     5461 catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga acggccgatt
     5521 ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat caaggcgatc
     5581 attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg ctggtttgga
     5641 aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg caagttgaca
     5701 aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt tggtttatgg
     5761 tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc tgattttctt
     5821 atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact ggattttaca
     5881 aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga tacgattcct
     5941 cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc gattaccctt
     6001 gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta tgaactactt
     6061 gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg tggaggtgga
     6121 cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga taacacggat
     6181 gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat ttcgtccatc
     6241 acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc gttgaaaaca
     6301 agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac aaagctgtct
     6361 cctgaagaag aaaagcaagt aaaagaccaa attatatcct atcaaacgat tcgtccggtg
     6421 atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat tgccgcatgg
     6481 atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat actagcttca
     6541 gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc actttatcag
     6601 gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg actttacttc
     6661 cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt atgagagaga
     6721 aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt tttagagtga
     6781 accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa gtagaaacca
     6841 catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg ccaaatcctt
     6901 cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt gatgaaatca
     6961 ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc aatacgatgt
     7021 atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct gagaaagagg
     7081 cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg acgacttttc
     7141 gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt gtagaacaag
     7201 caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg ttcactccgt
     7261 ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg gaggtttaca
     7321 aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt gggatcttca
     7381 gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg atcaaacctc
     7441 gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt gagcaagtag
     7501 ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa atctatcaac
     7561 agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa aatgtggcac
     7621 tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata gacgaagaaa
     7681 aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa atcaaagata
     7741 aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga catgagtggc
     7801 atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg gctatcataa
     7861 aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc acttggtatt
     7921 atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa tcccatggct
     7981 catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca gtacctcagt
     8041 ggcaggcggc tgttcttgat cgagcaaaaa cgaattttga aacattaaaa aaccatccaa
     8101 gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt cgacaaatga
     8161 acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg aattttccaa
     8221 atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg ccaccagaag
     8281 agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt gaatatatgc
     8341 atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt gatcgttatg
     8401 aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa gtgactgatc
     8461 caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa ccaagtgact
     8521 atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc gccatgcagg
     8581 aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac ggaaaaagtt
     8641 gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca ttatattttt
     8701 tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg gctataccgg
     8761 gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag cggatttgga
     8821 tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga aatagaagta
     8881 ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca atattcaagt
     8941 aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac agtaccaaaa
     9001 acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt caatatgcat
     9061 tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt cattatgcca
     9121 acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc ggatcggatg
     9181 gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc ataccttgtt
     9241 ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag aaatcaaacg
     9301 ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca gcaggatacg
     9361 ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc aacacatata
     9421 gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt aagaggagtc
     9481 gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag cagcgaagag
     9541 gaccatgaaa tcggctttat tattcagtag caatctttag attattattt gaaaaacgga
     9601 gtttacaaga caaagctgct ttattctata tgtcatgaac aagagagtaa aaaagttctc
     9661 agatactttt gttcatgact attttcttca ataaaaccaa
//
LOCUS       DANKAY010000100         9538 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000100 DANKAY010000000
VERSION     DANKAY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            241..2076
                     /gene="tdc"
                     /locus_tag="PWY98_002357"
     CDS             241..2076
                     /gene="tdc"
                     /locus_tag="PWY98_002357"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2038075.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2381..3859
                     /locus_tag="PWY98_002358"
     CDS             2381..3859
                     /locus_tag="PWY98_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2038076.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3825..5156
                     /locus_tag="PWY98_002359"
     CDS             3825..5156
                     /locus_tag="PWY98_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2038077.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5270..7822
                     /locus_tag="PWY98_002360"
     CDS             5270..7822
                     /locus_tag="PWY98_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2038078.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7829..9268)
                     /locus_tag="PWY98_002361"
     CDS             complement(7829..9268)
                     /locus_tag="PWY98_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038079.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         3005 a         1581 c         2033 g         2919 t
ORIGIN      
        1 ttgtttgttc gcttttttta ggttatctat tttagaagac tgatttattg aaaaattgtt
       61 cgcttttttg cgttgaatta tcagaaaaat ccgactttat ttatttttgt ttgtaaaaat
      121 ctacgtggat ttgatgttat aaaaaaaaaa ttctcatttt tggcgctata gtgaagaggt
      181 gttacagata aacacatgta tctgcaagac attgaccaac aaaatatgga gggaagaaaa
      241 atgaaagata tggatatcaa ggccgtcttt ataggcgaca aagctgaaaa tggaccagtt
      301 tataaaatgt tgttgaacaa gatggtagat gaacatcttg gatggagaga aaattatatt
      361 ccttcagata tgcctgcaat tagcgaagga gataagctca ctccagatta tctagccacc
      421 cgcgaccata tgatagaggt cttggatgaa gtcagtcagc gattacgcgc aggatcgatc
      481 ccatggcatt cagcaggccg ttactgggga caaatgaatg ctgaaacatt gatgcctgct
      541 ttattagctt acaactatgc gatgctgtgg aacccaaata atgttgcatt ggaatcttct
      601 atggcaactt cgcaaatgga agcagaagta ggacaggatt ttgcttcatt attcaatatg
      661 acagacggat ggggacacat tgcagcagat ggttctattg ctaatcttga aggattatgg
      721 tacgcccgtt gcatcaaatc gattccttta gctgtcaagg aagtgctgcc tgaaaaagtg
      781 aaaaaaatgt ctgagtggga acttttgaat ttatctgttg aagaaatttt ggaaatgacc
      841 gaaagcttta cggacgaaga aatggatgag gtcaaagcag cttcttcccg tagcggaaaa
      901 aatatccaaa gattagggaa atggctggtc cctcaaacaa aacactattc ttggatgaag
      961 gcgttagata tttgcggagt cggcttagat cagatggttg ctatcccagt tcaagaggat
     1021 tatcgaatgg atattaatgc tttggaaaaa acaattcgtg aattagctgg acaaaaaatc
     1081 ccaattctag gtgtggttgc tgtcgtagga acgacagaag aaggtcaagt agacagtgta
     1141 gataaaatcg tccaattaag agaaaggtta aaagatgaag ggatctattt ctatttacat
     1201 gttgatgccg catatggcgg ctatgcgcgt tcactgtttc taaacgaagc aggagaattt
     1261 gtgccgtatg cttccttagc tgaattcttt gaagaacatc atgttttcca ccactgcgta
     1321 acgattgaca aagaggtata tgaagggttc agagctattt cagaagcgga ttctgtcacg
     1381 atagatcctc ataagatggg ctatgtccct tatgctgctg gaggaatcgt aatcaaacat
     1441 aaaaatatga gaaatatcat ttcttatttc gcgccatatg tgtttgaaaa atcagtaaag
     1501 gctccagata tgttaggagc ttatattttg gaaggatcaa aagctggagc aaccgccgcc
     1561 gcagtctgga cagcacatcg tgtattgcca ttgaatgtga ccggatatgg tcaattgatt
     1621 ggggcttcaa tcgaggcagc tcaaagattc agagaatttc tcgatcatct gacttttact
     1681 gttaaaggaa aaacaatcga agtctatcca ttgaatcatc ctgattttaa tatggtcaat
     1741 tgggtattca aagaacaagg ctgtacagat ttgaacgcta tcaatgaatt aaatgaaaaa
     1801 atgttcgatc ggtcttctta tatggatggc gatgtttatg gtgaacggtt tattacttct
     1861 catactacat ttacacaaga agattacggt gattctccga ttcgttttgt tgaaagaatg
     1921 ggtctaacga aagaagaatg gaaaaaagaa cagaaaatca ctctgctacg tgcggctatc
     1981 atgactcctt atttgaatga tgatcgaatt ttcaattttt atacaaaaaa aatcgccaaa
     2041 gcaatggaaa agaaactaaa cgaaatcatc caatagaact gatttttgtg tgaaagaata
     2101 acaagagaca tatggagaac agacaattat ttgcttcatc aaaacaattg tatttccccc
     2161 tgtttttcat atattcctcg ccaagatatt cttttttata aaacgttcta taagttggat
     2221 cgttcgtttc atgtatcaaa cccagttcac taagatcgtt caatggatgt ttcggtgata
     2281 ctgggttttg tactgaatcg atgaaacctt gtcatttctg ttcttgaaca aaaataaaag
     2341 aaaacaaaaa catatgtcgc tattgaaaga aggaataaag atgaaagata ctggttcatc
     2401 aaagcaaatc acgaccatga cctttatcgg aatgacctgc gcccttgtag caagtgtacg
     2461 aaatattcca gatgtagcag caacgagctg gacgatgatt ttttacatgc tggttgcagt
     2521 attattgtac gcttttccaa tctcattgat ctctggtgaa tttgctggaa tgtttcccca
     2581 aaaaggtggt ccagaacttt gggtatcgaa tgctctagga aaaaaatggg ggttcgttgt
     2641 ctcatggctg ttatgggtcc aaatgtttcc tgggatggtg atggtcgctt cagcactcgc
     2701 tccgttgttt ggtaatatta tcgataatgt gccgcttggt ttgaacagca aatttacatt
     2761 agttgtcatt ttagtcgttt attggatcat cacttttttg aatttgaaat ttgatatggc
     2821 aaaaatcggc ggaaaagtcg gtgtttggtt aggattgtac gtgcctttga cgatgatgct
     2881 cttattaggg tttgctgcgt ggatcaaagt tgggatcgta ccaaccggaa cattaggcag
     2941 tttttcatgg gacaaattaa taccagatac tacgactgca tcctcatttg tttattttgc
     3001 gccgatcatg tttattttta ctggtatcga aatgtcctct gtgtatatca ctcgattgga
     3061 aaatccagtt aaaacgtata ttcgcggagt atttgccgcg ttaattttta tatttttcgt
     3121 caatattttg aatgctttag ttgtagcaaa tgtcgtacca aaaggacaaa tggaattgaa
     3181 taatatcgcg caatctattt cgatttattg ccaaattctt ggtttgccac atcttattgt
     3241 gaatctgttt agtttacttg tcttcattgg tgtagcagtc caattatctg cttgggcctc
     3301 aggaccagca aagacagtga cagaaagtgc tagaaaaggt gcgtatccac caaaattcaa
     3361 tttttggaag acgaatcaat ttgacgtatc caaatccgtt attttgactc agtctgtcat
     3421 tatctcaatc tttgcacttt tttatttgct gattcctggc gtcaatcaag catttttgat
     3481 gctcgtaaat tcaactactg ttatctactg tatcgtctat gtcatcatgg ggatcgcagt
     3541 tttgagattg cgttatacac atgcaaagtt gaatcgacca tttagaattg gaaaaaaaga
     3601 gaaaaagagc aatcttggag tttggactgt cgttattgtt ttatttgcgg caatcggatt
     3661 ttctgtcgga ctgacgatga aagctggaac atggatcaat ctaattgcag tcacagccat
     3721 cagtgtcatt ttgtttgtag taccactctg tattgaaaaa ataaagaaac cttcttggga
     3781 acaggaagtc gccatgctgc ttgaaaaaga aaaaggtgaa cttgatggaa aaaccggcaa
     3841 tgaaacggac tctaactaat cgaaatatcc agttaatcgc attaggtggc gcaattggaa
     3901 caggattatt tttaggttca gcaaaggcaa ttgcatcagc agggccagct gttattctcg
     3961 tttatttgat atgcggtttg gctatttatg gtttgatgcg ggccatgggt gatttatttt
     4021 tagcaaatag tacatttaac tctatcagtg atttcgttga ctattattta ggcaaaaagt
     4081 gggcattttt tgtcaattgg acttattggc tttgctggct tggagctgga cttgcggaat
     4141 taacagcaat aggattgtat atccatttct ggtttgtaca tgtaccagct ttgataagcg
     4201 gaggtagcgc actaatgatc ttaatggcag tcaaccttgt tgcagtcaag tggtttggtg
     4261 aattggaatc ttttttctca atcatcaaga tcttggggat tttccttttt atcttattgg
     4321 gtataggatt gagtgtctca gattttcata cccagtctat cggaatcaaa gaaaattttg
     4381 gacagatcga tgaattacta ccgtttggct ttgctggaat ggtagtctca tttcctatgg
     4441 ttttattcag ttttgctgga attgaaatga tcggcttaac cgttggcgag acaagtgatc
     4501 cgcaaaagaa tttgaaaaaa gcaatcaact gtctgccttg gcgtattctc tttttctata
     4561 taggaacgat tttgtcgatt ttatttgttg ttccatggca ttgtttagat ttaaaagaaa
     4621 gtcctttagt gactatgctg caagtgatcc attgccaagg cggagcagca attgtcaatc
     4681 taatgatttt ggttgcttct ttgtcctcag caaatagtgc agtctacagt actagcagga
     4741 ttttatatca aacagcaaaa gaaaaaagtt tgccccattg gttttgtgcg ctttctaaga
     4801 aaggcgtacc tttacacggt attttaatta caggaatgtt attttttgcc ggtctttgtc
     4861 tacatttttt tgtagctgat agtcgattgt tttttcagct tttggcaggc atgacaacga
     4921 gttgttttct atttgtgtgg tcttcgatct tgtgtgcaca tgttcaattt attaaagaaa
     4981 agaaacggaa cactacacaa agatataaag atcttgcttt gttggtgttt tttacttcaa
     5041 ttgctttcag cttgttattc gaaagcacga cacgctttat tcctttttta ttggttatct
     5101 ggtttgtatt cttaaatttt gtatataaaa gaataagcaa tagaaatatc agttaggccc
     5161 agatacattt tgcaaaacga attatttcag acccccctga aataatcgat acgcctgaaa
     5221 aaacttccct gcgtataata acagtatcaa ttaaacagga ggggatagga tgaactggta
     5281 cagattgacg gtagaagatg tattgaaaaa ctatcaaaca tcaacagaag gcttgtcgca
     5341 agaagaacga cagaaacgcc tgactgatca aggatataat gaaataaaag caaggcagca
     5401 gacaaaacga tggaaaaaaa tagcaaagca ttttactgat ctgctgatga tcgtgttgat
     5461 cattgcctct tttttgaaat ttgcttctag tgaatatatt gaaggaagta ttattttatt
     5521 cgtagtgatc gtcaatggac tcgttagtta ctggcaagaa agaaaagcgg aagaatcatt
     5581 gaatggtttg aagcagctga tgggacaaga agcgatagta ctatcaaacg ggatgcaaga
     5641 aaaaatccct gcgaaagaac tggtaaaagg agatgtcatt gtcttacgtg ccggagatgt
     5701 cgttccagca gatatccgat taatcgaagc acatgattta ttagtggaag aatcgatttt
     5761 gacgggagaa tcagaagcat ctgaaaaaag tcatgccgtt ttgacagaag aagaatcgat
     5821 cggcgaccag aaaaatatgg gtttttcagg aacgcttgta caatctggtt ctgcagtcgg
     5881 tgtcgttgta gaaacaggaa acacgacaga aatcggtaaa atcaatcaag cattgcaatc
     5941 ggtcgaacaa caaacgacgc ctctgattaa aaaaatcaac cgattaaaca aacaaatttt
     6001 tcaaggcatt ctttgtttga gcctattctt agttattttt acgacatttc gatatggtat
     6061 ggattggcat atccttcttt cggctacgat tgctttggtg gtgtcaatgg tacccgaagg
     6121 attgccagct gttttgacca tgattctatc cgttggagta catgaaatgg caaaagaaaa
     6181 ggcgatcatc aaaggacttc cttcggtaga aacattaggt tctatgacag tgatctgttc
     6241 agataagaca ggaacattga caaaaaatga aatgacagtt gtggatgtag ctgcaaaaga
     6301 agaagcctgc gtgttgtcca ttatgaaaaa ctgtcaggaa ttaaaaacaa aagaagaaca
     6361 gaaaacagaa aatttaagtg gcaatccgac agaagttgcg ctgattcgac acacagaaac
     6421 agccaatcta cctttgaaaa aaatagaagc aaagattccg ttcagttctg aatacaaata
     6481 tatggcaacg atgcatgcgg aggaaaaagg agcggtgatt tacgtcaaag gtgcaccaga
     6541 agtattattt gctaaatcaa ctctttcttc tgcagaacaa gaagcatgga gtcagacagc
     6601 tgcagaattt gcgagaaaag gtcagcgtgt tttaggattt gcttataaaa aagtagattc
     6661 gaaacaggaa ctcacgcatg agactttaac ccaattaact tttgcaggga tcgccggatt
     6721 gatcgatccg ccaaaagaaa gtgcagtcaa agctgtaaag gaatgccaac aagcagggat
     6781 ctcagtgaaa atgattacag gggatcacaa agacacagca aaagcgattg cggaacaaat
     6841 tggcttaaaa catactgcca aagttttaga agggatcgat ttggatctta tgtctgatga
     6901 agagttgatt cagcaagtgc caatagtcga tgtctttgct cgaacgacac cagaacataa
     6961 actgcgaatt gttaaagctt tgcagaaaaa tggagaaatc gtcggaatga ccggagacgg
     7021 cgtaaatgat gcacctgctt tgaaaagatc cgatgtagga attgctatgg gaatcaaagg
     7081 tagtgaagta agcaaacagg cagctgatat ggttttagga gatgataatt tccatacgat
     7141 tgcaaaagcc gtaaaagagg gccggcggat tttggataat ttgcaaaaaa ccatcaattt
     7201 cttcttgcca actgcattag ctcaaggatt gatcttgatt tgggcactta tgctgaatcg
     7261 cccgctacct ttgtccccag tacagatttt atgggtcaat atggtaacga caatcacttt
     7321 atcttatgca ctagggttcg agtcgccaga tccagaaatc atgaagcgcc caccaagaga
     7381 tccaaagcaa ggaatcctat ctggttatca tttgtttcgg atcatctatg tctccctact
     7441 gatcatggtt cctgcctatt tattggctat gcagtttgaa gggcaaagct tacagcaaac
     7501 cattcttctg caaaatatcg tcttagctca agctgtttac atgatcaatt gcagagagtt
     7561 gtccaagccg tcattgaacc aaggtctttt gcaaaacaaa gcgctttttc tttcattagg
     7621 tatccttgct ttattgcaag ggatggttat ttttctacca gtcggccagc agctgatcgg
     7681 tacagtaagt ttaacgatga cacaacagtt attgattgga gcaaatgtga ttcttctatt
     7741 cttagttgta gaaatagaaa aagggatcat gaacaaactg tttcgcaaaa aagaaaaaac
     7801 agctagagaa tacagtcgct agccgttact ataaaaaagt tttgaaagtg agttcagtca
     7861 aacgattgag ctcatttttc gttggaatag aagaaatgta tacaataggg atatctagag
     7921 aatctagagg gaaattggaa atcaagatat ctgttttttc aaaagaaata gtggaaagat
     7981 cggttagttc gataggtaag aagcagacac gttttcctaa gtaatctttt aattcctgct
     8041 gcaagaacgc cttccatgaa ggttctcctt ggaaaataaa atagacattg aacaaattcg
     8101 gtttgataga aaaaatcgtt tgttggatca gtagagacaa gagttggaga aagtaatttg
     8161 gttcattgat ttctatttct ttttttagat cgttcagtaa attctctttc tgttgataca
     8221 agtgtgcata ttgctgatca tatgtttcta aaaaacgatg gtattgacgg tccaatgctt
     8281 caatcaatat cggcgattca tgatatttta atatgaggag agccaagtta tcagtcagag
     8341 atgttttttt tagttgatca agagacagct ctgaagctag ttcctctaca aacgtttcga
     8401 ggacctctaa aaatggagaa tgatgcaggc agcgagcggt ttcttgtcca tagttattgt
     8461 tgtaattcca actttccaga aagcagaaag aagcaaaaga aagctcagtg tctttaagat
     8521 aaataccttc cgttaaatac aattttttca atgcagagtc atacagggag gagagtgtgc
     8581 tgttggtatg gatagaagaa tctaatcggc aattcgcttt tatacggatc gtattgataa
     8641 agaaccaaca tgttccgaaa atctcttctg tttcaaccgc tagttcagtt tgactgaggt
     8701 ttcttaaaaa ttgaaaataa tgggattcat ggatcgaata atcctcgaaa aaatagttgt
     8761 tatgagtgaa aggaatcagc aatcgatgat aaaaagtacg aatcgcacgc tcttttccaa
     8821 gaatacaagc tgtatatttc gtcaaatcga tctgcaagtc gtaacttgct aaacgctgat
     8881 tgattttagt cacatgtctt ttcaatgtcc cataagaaag cccatggtcg gctagaaaat
     8941 cagggacagc aacttttttt gtaaaaagca gatttttcag cagttcgaca ctgactgatt
     9001 gtttgaaaaa aatctcccaa aggtcatttg ttaataaatt ttgctggttt tgcaaactga
     9061 ttcctgcttg atcgctgatg atgtcccagc cttcgggcag ctgagagcga atagattgaa
     9121 ggtcagaaaa aatcgttcgt tcggttgtcc ctatgatttc agctaattca tttacggtta
     9181 tctttgtatg attcagcaat tgttctacta aagaaacatg acgtcgaata tctttttcgg
     9241 taatgatttt tttcattagt gaatacatca atcagactcc cttctattct aagaatacgg
     9301 agaaaataaa cggtggtcaa gtttcaactg atttttcaat cattgtttat ttttctggag
     9361 catttactga tgataagaat ttcatcatga acttgaaaaa tgataccact aaaatcaaaa
     9421 aataggtggg caaacaataa taaacatttt aatagtagat ggtagcaaga ccaacacagg
     9481 cggattgaac atttaagagt tgataaaaaa gaaaaatatc gaagtatttg attagcaa
//
LOCUS       DANKAY010000101         9318 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000101 DANKAY010000000
VERSION     DANKAY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(29..526)
                     /locus_tag="PWY98_002362"
     CDS             complement(29..526)
                     /locus_tag="PWY98_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2038080.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(656..1366)
                     /gene="budA"
                     /locus_tag="PWY98_002363"
     CDS             complement(656..1366)
                     /gene="budA"
                     /locus_tag="PWY98_002363"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2038081.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1379..3043)
                     /gene="alsS"
                     /locus_tag="PWY98_002364"
     CDS             complement(1379..3043)
                     /gene="alsS"
                     /locus_tag="PWY98_002364"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2038082.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3248..3910)
                     /locus_tag="PWY98_002365"
     CDS             complement(3248..3910)
                     /locus_tag="PWY98_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2038083.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3920..4735)
                     /locus_tag="PWY98_002366"
     CDS             complement(3920..4735)
                     /locus_tag="PWY98_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2038084.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4997..5440)
                     /locus_tag="PWY98_002367"
     CDS             complement(4997..5440)
                     /locus_tag="PWY98_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2038085.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5574..5912)
                     /locus_tag="PWY98_002368"
     CDS             complement(5574..5912)
                     /locus_tag="PWY98_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2038086.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5900..6277)
                     /locus_tag="PWY98_002369"
     CDS             complement(5900..6277)
                     /locus_tag="PWY98_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2038087.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6501..7694
                     /locus_tag="PWY98_002370"
     CDS             6501..7694
                     /locus_tag="PWY98_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2038088.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7860..8282)
                     /locus_tag="PWY98_002371"
     CDS             complement(7860..8282)
                     /locus_tag="PWY98_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038089.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8677..8871
                     /locus_tag="PWY98_002372"
     CDS             8677..8871
                     /locus_tag="PWY98_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038090.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8868..>9318
                     /locus_tag="PWY98_002373"
     CDS             8868..>9318
                     /locus_tag="PWY98_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038091.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEA"
BASE COUNT         2831 a         1882 c         1588 g         3017 t
ORIGIN      
        1 ctcttctttt ttgtttcaat agacgatttt agttattaat aaatgaaaaa gcatttatat
       61 taaaatttgc taataaaaga tctacatatt tttctagaaa tgcttgatcg atttcatcat
      121 accctttcgt tactccgcaa tcgatatcta aaacaccgat taaattacct tcttttacca
      181 ttggcactac gatttctgat tgtgcggctg aatcgcagga aatgtaattt gcatgggttt
      241 tgacatcatc aacgatcaat gtttcttgtt tttcagctga ttctccacaa actccttttc
      301 ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg tcctaaaatc aattcattac
      361 catcgaataa ataatatcct gcaaagacag tctcaggcaa agtctcattc aacaatgcag
      421 atgaatttgc taaatttgct aaccagttat cttctgcttc taataatcct tgttgctgta
      481 ataataaaat ttcgtatgca tcttttttct gtttttctgt ccacatgata aaaaccgctc
      541 ctttttgatc tatggagaag attatagctc agtttttttg taaacgctag gtttcctcct
      601 caaaaaaacg agatacgtca ggttaatcac cttgaacgca tctcgctgtc tttttctatt
      661 ctgcttgttg gatttctgct gctaaatcag cataatcaat cgtttcgtga acaaaagtcg
      721 gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg gctaatctca acttttcctt
      781 cagccacttc aaaatccatt acatgtccgc caaatgtatg tgtatcatcg ataaaatgca
      841 agtgaaatcc cgctgcagca actccttgga ataattcagg cgtaaagaac ccgactaatg
      901 ttccttggac attgctttgt tggaattctg gttgagactc tgaaattttt gctagtcttc
      961 tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat atttttgaat aatccagtta
     1021 tcttcactgc caaaaatagg ttttccccgg cttctcttac taatatgtct tttttcaatt
     1081 gttctccatc tgcagttttt gagacagaaa aactagtatc tggcgtaaaa tctgtaattg
     1141 ctgcataggg ggtaagttca cttccatcta attgggcaac tgagccatct gatcttcctt
     1201 gataagctac gccatctaaa aagatgactt caccatcaag cccatgcaat gttcccaatc
     1261 ccagagttcc tttttctaat atatgagcaa tcgtttctgt tccatccatc aagccagcca
     1321 tcaaagctcc taatgtcccg tgctggtata agattttttc agtcatgtcg acacttcctt
     1381 aatagaattg atctggtaag attgtttctc ccaatttctc attatcacgg taatcaatcg
     1441 gaatatcaat aattacgggt ccttctgtcg caaatccttc ttctaaagct ttttctaatt
     1501 cctctacact tgtagctcgg attcctttag cgccaaaagc ttctgcatat ttcacaaaat
     1561 caactggacc aaagtctaca cctgacgccc gctgatattt cattttttct tggaactcta
     1621 ccatattgta gtgtccatca ttccaaatca aatgaacgat attcaatttt ttacgtaccg
     1681 ccgtttctaa atcctgggca gagaataaaa atcccccatc gccggaaact gatacgattt
     1741 gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt
     1801 gcataccatt actgaataac aaatgacgag gttcataact tctaaagtgg cgtgccatcc
     1861 aaatataatg actacctaca tctacggtaa cagtcatatc atctgttact tttgactgaa
     1921 gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc tccttttgat tgaacaatgt
     1981 cccgctccgt taattctgct tgaagagatg ctaaatattc ttttgcatct tctgacactt
     2041 gttgaggctc taatgaacct gtcaataaat caagagtggc tgagatatca ccgatcaatt
     2101 ctctttcagg ctgcataaaa ggatcgattt cagctggcgc ttcatcgatc acaatgatcc
     2161 gcgcatcttt ttccgcattc cagtttcttg cttcatattc gattggatca tagccaatag
     2221 caataactag atcacttcgt ttcaaaagca tatctcctgg ttggtttcta aataaaccca
     2281 cacgaccaaa gaaatgcgct tctaattcac gtgagatcac tccagctgct tggaatgtct
     2341 ctacaacagg caatgctgtc tttccaacta attgcctgat ggctaacgtt tctttttcac
     2401 ttgatcctcg catacctact aatagtaccg gtagttttgc ttctctaatg cgttcagcta
     2461 ggtaacggat atcatctgca gaagcagaac ctaatttagg atcgctcaat ggcttgatga
     2521 cattcccttg gacaggtgca tccactacat cttgcgggat actgataaag ctcgcgcctt
     2581 tttttgaact cttagccata cgataagcat tggcaatcac ttctgaaatt gtttctggat
     2641 cttggatttc tgcactgtat tttgtgattg gctggaacag tgctgcatta tccatgcttt
     2701 gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt
     2761 caccttctgc tgtcgctgtt accaaacctg tagctaagtt actagcacca ggaccacttg
     2821 ttgcgataac tactcctggc tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag
     2881 cattttgttc gtgacgagtc acgattaatt caggaccttg atcctctaat tcattaaata
     2941 caccatcgat ttttgcacct ggtataccga atacataatc aacatgatga ttaatcaaac
     3001 tttctactac agatttcgaa ccttgtttca ttttttcgtc cattgaacat cgtccttttt
     3061 atgctttatt tttaattatt agttagaaat aatcacaaat aattgataca gtttctaaac
     3121 ctgtttcatt caaaagtata gcacgtttta tttcatttgt ttagccatat gtttcaaaaa
     3181 atgaaaaaga atgacagttt ttacataaaa actgtcattc ttaattgaaa gtttcaattt
     3241 ataaagtcta cagatccata gttctcatca atttacgatt gttgaagtaa atcaaaccaa
     3301 atacgataga aggacctagt agaatacacc attggggctt tacgaaaaag aagagaaaca
     3361 aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca ttttttcaac gtatagtaga
     3421 acgctatccg tagaacttga tttggtttcg acgctggatt tctcacctga aaataccagt
     3481 agttgatgct aaaggaaact gataaacaag caagtaaaac aaacaacgga atcaaccatt
     3541 ttccataaga aaaacgtata aaaaagaaac aatcaaaaat cattatcaca ctaactatcc
     3601 aagcaaatag ccaataaaaa aatcccttta gccaaaattg gcgaaacgtc tttatgaaca
     3661 aacgtactgg tgaaaaactt ttttcttttg ctaattcttc tgtcatacct atcatcacga
     3721 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
     3781 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
     3841 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
     3901 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
     3961 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
     4021 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
     4081 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
     4141 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
     4201 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
     4261 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
     4321 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
     4381 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
     4441 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
     4501 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
     4561 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
     4621 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
     4681 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     4741 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     4801 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     4861 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     4921 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     4981 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     5041 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     5101 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     5161 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     5221 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     5281 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     5341 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     5401 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     5461 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     5521 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     5581 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     5641 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     5701 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     5761 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     5821 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     5881 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     5941 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     6001 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     6061 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     6121 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     6181 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     6241 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     6301 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     6361 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     6421 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     6481 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     6541 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     6601 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     6661 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     6721 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     6781 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     6841 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     6901 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     6961 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     7021 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     7081 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     7141 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     7201 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     7261 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     7321 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     7381 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     7441 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     7501 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     7561 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     7621 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     7681 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     7741 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     7801 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     7861 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     7921 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     7981 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     8041 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     8101 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     8161 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     8221 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     8281 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     8341 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     8401 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     8461 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     8521 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     8581 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     8641 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     8701 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     8761 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     8821 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     8881 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     8941 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     9001 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     9061 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     9121 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     9181 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     9241 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     9301 ccactttaag cgaagctc
//
LOCUS       DANKAY010000102         9184 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000102 DANKAY010000000
VERSION     DANKAY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(21..1052)
                     /locus_tag="PWY98_002374"
     CDS             complement(21..1052)
                     /locus_tag="PWY98_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2038092.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1059..1889)
                     /locus_tag="PWY98_002375"
     CDS             complement(1059..1889)
                     /locus_tag="PWY98_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038093.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(1886..2701)
                     /locus_tag="PWY98_002376"
     CDS             complement(1886..2701)
                     /locus_tag="PWY98_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2038094.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(2698..3522)
                     /locus_tag="PWY98_002377"
     CDS             complement(2698..3522)
                     /locus_tag="PWY98_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL2038095.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(3512..4612)
                     /locus_tag="PWY98_002378"
     CDS             complement(3512..4612)
                     /locus_tag="PWY98_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2038096.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4790..5575
                     /locus_tag="PWY98_002379"
     CDS             4790..5575
                     /locus_tag="PWY98_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL2038097.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            5608..5991
                     /locus_tag="PWY98_002380"
     CDS             5608..5991
                     /locus_tag="PWY98_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2038098.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6067..6198
                     /locus_tag="PWY98_002381"
     CDS             6067..6198
                     /locus_tag="PWY98_002381"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2038099.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6167..6403
                     /locus_tag="PWY98_002382"
     CDS             6167..6403
                     /locus_tag="PWY98_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2038100.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(6481..7452)
                     /locus_tag="PWY98_002383"
     CDS             complement(6481..7452)
                     /locus_tag="PWY98_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2038101.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            8086..>9184
                     /locus_tag="PWY98_002384"
     CDS             8086..>9184
                     /locus_tag="PWY98_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HDL2038102.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPPFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT         2698 a         2104 c         1796 g         2586 t
ORIGIN      
        1 aaatgtttgc cgactataca ctagctttgt tgtgcaaaat gctgagtatc aatttccaca
       61 gggattgcta gaatatcttt cagctgaatg tataaggcat cgctaatttt ctcttcccga
      121 tcgttcaacc gatccactaa aatttctttg ccctccagcg tttgaaaaac ataccgttga
      181 atgttgccta aaataaatga ttccttccat agaaccggaa tcttaacccc ctgaggaacc
      241 ggttcaaatt gaatcagctc cggtcttaaa taataaatgc agcgattgcc cttagcggaa
      301 aactcgtggg tatactgatt cagctcatac cattcgaaac gattatagtt gccgataaat
      361 tccgcaacaa atcggctctg aggattctga tacaattcct tcgaggtgga aacctgagcc
      421 acccgcccag cttccatcac aaaaatccga tcactgattc gcatcgcctc ttcttggtca
      481 tgagtcacaa aaatcatcgt catccctagc gattgctgga tagcgcgcaa atcccgctgc
      541 agctgttttc taattcgggc atccaaagca cttaatggct catccagcag caggactttc
      601 ggttcagtga ccaatgagcg agccagtgcc acccgttgtc gttgaccgcc agaaagaaac
      661 tggacatttt tatctgcctg ctccgtcata tccaccaatg atagcatctc aaaaaccttt
      721 tcctgaatca cctcaggact cagcttcttt accttcaaac caaaggctac attttcaaaa
      781 acagtcatag ttggaaacaa tgcatactgt tgaaaaacaa agccgatatt gcgatccttc
      841 gtaggcttat cggcaatatt ctcattgtcc agcacaatct caccgctctt tggttgttcc
      901 aatcccgtga tacagcgcag caacgtagac tttccgcacc cgctgggacc tagtaaagta
      961 accagctcac ccttttcaat ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa
     1021 aacagattga gatcatgtat ttctaaaaac attttccgtc acaactcctt taagggcttc
     1081 tttttcgact gcttctcttc cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa
     1141 gtcagcataa aggtagttaa agcaattccc accataaaca gcaaatacaa aaccatcaca
     1201 gcgctagcca aatggccgtt ttcgttcatc cgccgcagca tatagatgcg taccgtctga
     1261 aatcgaccgc caatcaacag gttcgtcagc atgtactccc caaaaaccga agaaaaaatc
     1321 atcagagaaa ctagggtcac accgatacgg atgtttggaa acagcacctg gataataatc
     1381 gtagacatcg gggcgcccaa ggtttctgct gcatccacta attccttcag attaattaag
     1441 cgcatctggt tgttaagact aagatagaca atcggcaacg ccgtaataaa caatgcacct
     1501 accaacacta caaacattgg gataccagtt tcagcatacg ttcgaagcaa agccgttacc
     1561 aaaatcactc ctggtaaagc atacggcagc aggagcagct tttccagcca acgattcaaa
     1621 cgagggaaat acaaatgaat ccaaataatg gtgggaatca tcaacaacag aatcgtcacc
     1681 aagaccacac ctgctaataa aagtgagcga cccaccgcag caataaattc cctgtccgtc
     1741 agcagctgtt ggaaccattg caaggtaaaa tcactaggta aaagcgattt tgaccattta
     1801 gtggagctgg catacaaaat cgttccaata atcggcagca agtaatacag gacaaagcag
     1861 aacaaaatcg gttttattaa ctttttcacg tctgcaccac cttgcctttc ttttgaataa
     1921 tcaacattgc cgttaccagc aggacaccta aagcgactgc caacgcactg cctaaattag
     1981 gcttggcgaa cacatctccc gacactaaag ccgcaattcg aatcgttaaa agatttacat
     2041 tactgctggt cagggcatac gctgtttcat aggtccccat gccattagca aataagatga
     2101 caaaggtgct ggcaatcgat ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg
     2161 ctgatgaagc gcccagcaat tgtgcggact ccagccattc ctttttcact tccttcatgc
     2221 tgggatacaa aaacaagacc cctaacggaa tttggaaata ggtatacgtg atggtcaatc
     2281 cccaccaaga atacaaattg aagtccgcta gaaatggcaa aatcattttc agcacaccta
     2341 cattccccaa caaaataata aaagagaatg ccagcggaat cccagcaaaa ttagctgcca
     2401 aattggaaac aaaggtgatt ttctcctgca atttagtcga tagattcatt aagcataaac
     2461 ataaaataaa cgtcccgatt aacccaatca cactggagct gacagcaatc agtacgctgt
     2521 taacaaaggc ctgtgtgtaa tatttgttgc tgaaaatatc actgtaattg gccaatgtcc
     2581 actggttagt taaatcactg tgcaagctgc acagaaccat aaaaagcagc ggcagcagta
     2641 aaaatgcaca gaagataaag gaggcaggaa ccagaaccaa gcgttttttc atctctttca
     2701 tcccctcatc aattcttgaa tgtcctgcat tttctctgac ggtgcaattc ctaataacca
     2761 gcaaaacaat ggagccgcca gcagctgtgg tacagtttgt tccttcagct tttggcgttt
     2821 atttgaaatc aaataaaaag gaacttcacg atgtgcagcc aagctgccgc catgcagtcc
     2881 gtgcgcatcc atcccgtgat ccgcagtcac cacgacttga taaccttttt gcagccattc
     2941 aggaaggtaa atagccaata atctgtccgc ctgattaacc gctgctgcat attcatgaga
     3001 atcggcggta aatttgtgac cgacatcatc aatattcatc gaatgtacga ccaaaaagtc
     3061 tggctgatac tgattcacta aataatgggc atccgcaaat aaatgcgaat cagggtaagt
     3121 gtcctcacta taaaagatgc catgctgaat cagtcgctcc tcctgatgct ggatgcgatc
     3181 aaccaaacga ttataggggg cttgattgta caactcgctg taccagtggt aagcggcggc
     3241 tcctgttttt ccgccagctt ccttaactaa ttcaaacagc gaccactctt tggatcgctg
     3301 agcatggtaa ttactataga tgccattttc ataggctgga acgccggtca tcagtacttc
     3361 gtataacgga cgtgaattgc tgggcagctc actttttacc ttcaataagc ttgcttgctg
     3421 gtgctcaact aaatgattca tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc
     3481 gtctagtata actacaatca gtttgtcaag tctactttgc ataggtcagt acctccgcct
     3541 gccaaagctc tggaagctct acagaggtct tgttccaagc ttcattgtct tcaactggca
     3601 ccgccgcttc ataagcatcc tccggcagta atttttcagc tacttcttta ggcagctcta
     3661 ccttgcgaat aggtcgcgca tagccctttg ccaaattgat ttgcccttca tcagataaaa
     3721 tatattcccg tgctagcttt gccgcattgg gatgaggggc atttttattg attaaggttg
     3781 tatacccgct aatgatggag ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa
     3841 tttggtcacg ataatttagt ccattgaagt cccaaacaac tgccacatcg atttcacctt
     3901 tttcaagatt tgctacgctg gcatcaacgg tggacaaacg gcctttttct gccatttttt
     3961 tgtagtaatt cagacctggc tgaatatcct tttcgtcacc tccgttggca atcgccgcag
     4021 ccaatacagc aaattgtgct tggttggcaa tcgtcacatc ccccacgcta acttgatagt
     4081 caccctcttg cagctccttc cagctagtcg gtgcttgctt cacattctct ttatcggtga
     4141 taaaggaaat ggttcccgta tagcttaaca accaatcccc atcatcatcc tttgcccaat
     4201 ccggaatttc ctcccaatag ttggtcttat aaggtaaggt taactctttg ctttcagcca
     4261 acggaccgaa gctgataccg acatcaccaa tatcggcggt tccatttttt ccttctgatt
     4321 caaacttagc caattcttct gcactggaca tatccgtatc cacgtgactt aagccgtatt
     4381 ctttttcaag gtcgttccaa gtgcctaccc aatttgccca agtatccggc attcccaagc
     4441 tggcaatctc tccttcttcc ttggcttttt ctataatcgt atctaaagag tcgtctgccg
     4501 cagccgattg cttcgttgct tcacctgagc cgcagcctgt cagcaggata cttactgttg
     4561 ctgcacaaaa taaaccgata gtcatgttac gaattttttc attcattttc attcattctt
     4621 ctcccctttc gataattcca ttctaacaaa gctttgtaat gagagaatca gtttcttgaa
     4681 aaggtttcat gaacaatgta aaggttgtat atggtttgta aatttcgacg aattcccgtt
     4741 aaccttctaa agcttttgcg gtatgatagt agaaaaaagg aggctcccta tgcagtatta
     4801 tgatcagcat ctccacacgt atttttcctt tgattcagag gaaaaatttg aaaactattt
     4861 agcctatcag ccggagtttt tcgtttctac ggatcacttt gatttaaaca atccatctac
     4921 cggaaaagat gacattccgg attatccagc ctatataaac aaactggact ctttaaaaga
     4981 aacctattcc accaaatttc tgcgaggtat cgagattggc gttgttccag gacaagaaga
     5041 gcggatcgtg gattacctcg ctcagcatcc ttatgattta aagctggtaa gtattcatca
     5101 aaatggccgc tttgattata tggacgatgt ggttttaacc aaagatccca ttcaaactac
     5161 caaggaatat tttgatcaga tgttgcaggt cttgacggat tttccagaag gagatatttt
     5221 gactcacttt gattatggat tacgccgctt caccttcaca gcggaagaat tcgcggagca
     5281 ttttgaggct cagctgctaa cgattttcaa aaaggttgtt gaacgagaat tggcgatgga
     5341 gttaaatgcc aagagcttct tgaaatacgg caacgaagac ctttaccgtt atgccgttcc
     5401 tttgtatcaa agtgtcggcg gaaagctctt taccttaggc tcggatgctc atgttgccga
     5461 agattatcag ctgggcttta aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa
     5521 gttgctagtc attcaaggaa aagaacgccg cctgacgccg ctgccgattt tgtgattcct
     5581 atataataga agaaagtggt gaaaaaaatg agcaacctgt gggactacaa tcaagaggca
     5641 cccatccatt atttaattgc gcgacattgg gatgcgttga aaattgaggc ggtttgccga
     5701 tccttgttag cagccgttcc aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg
     5761 cagcgggaaa aagttcaggc ttattttgca gcctttaaag atcagccttt ggaatatttg
     5821 catgcgcagt ttcatctttt ttatcaagta gccgccccag atgactacaa cgatttacga
     5881 ggacagctgc aattgacctt tcaagcggat gaaaccgcct ataccgtttt attgggaatg
     5941 gctcgccttg gtgatcaggc gaaggtcgag tggcggattt tcgatatttg atggcaggat
     6001 caaggcgttt cagttgcaaa ccttcctctc tttcaattag aaaaaccgca gcaggaagaa
     6061 gtttgtgtgc tggtcaccga tgctggtgaa attcgtctcc gcttatttcc tgaccaagct
     6121 cccctggctg tagccaactg gcgctcccta gctgagcaag gcgtttatga caacacacct
     6181 ttcggctcgt gtcattaagg aatttgttat tcaaggtggc gctttggatg gttctggtga
     6241 tgaagctgtt tccagctatg agggcttttt cgctgatgaa gtgcatcgcg gactttatca
     6301 ttttaatggc gccttggcct taggggacca cggcccccac accaacggca accagttttt
     6361 tattgtgcaa aacacaaagg cgcaggctga cctgctaatg taaagcaagc atacttggag
     6421 cttggaggac taccggagct ggatggtcgt tatactgttt ttggacaggt ataccaaggg
     6481 ttagaaatcg tagcagcgat cgcagctcag ccgacaaaca gtgaagatgt accgattgag
     6541 ccagtgttca ttcgacaaat taacttcgaa aaaacatcgt aaaaagacac tcactgactt
     6601 cagcaaaagc tctattcttg agctggctgc agctagtgag tgtctttcat ttatccataa
     6661 gataaagctt ggattcaata aataaataaa tgttttggtt aaagctgtga ccagcaaatg
     6721 aggaaccgtg cgagccagtt gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg
     6781 cgtgtctgcc aacggatact ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg
     6841 gtacggtagt aattctaacg gtacgaaccg atcaagatct gccacaaaac aagcaatcgt
     6901 cagcagctcg gtcaggttca tattgatacc tggaaccaca ggaactcgaa ttcgcacttc
     6961 gcagttagga tactcaatta aaaagcgacg aatattcttc aaaatttgtt ggttatctaa
     7021 gccggtccat tgtttgtgga gcttgctggt aaacattttt acatccacaa acaatgtatc
     7081 cacataggga gctacctttt ggatttcatt ataagcacca caaaagctgg tttccagtac
     7141 ggtattgatt ccaatgtatt tcgatttttg caaaatttct ttagcaaaat ccgcctgaac
     7201 cagtgcctcg cctccgctaa tggtcactcc gccatccgag tgaaagtaaa aaacagcatc
     7261 cttctcaatc tcatccatta cctcttcagc ggtcatggtc tggccaaatc ctttttcaat
     7321 ctgcttgctt tgtgattcgg gaatcgaaca ccaggcacag cgtaatgggc agccctttaa
     7381 ataaacaacg gtccgtaaac cagcaccatc atgtagagaa ccctgttcta ttcttaatac
     7441 tgttccttcc atatagattt ttgaattaca cactagttta aactaaaaca cacaaggaaa
     7501 aacttaccat ttaatcataa aattagctca tataaatata ctaggatgta aaatagactg
     7561 gtcaaaatct ggtcaaaaaa gaggactaat acaggtagtc ctcttttttg ttattttaat
     7621 attctaaatc attttttatt ggtttattcc cttattcgat gtagactcaa atcactatat
     7681 cactagatat caaacggcgt catacggaat caaaaacatc aaatttgacc tgttaaatgg
     7741 tcattgaata acattcaaaa tcaataaaac ggagtcagac acggagtcaa aaacgctgag
     7801 agtaaaaaat atatacagcc ttgtaggtaa agtaccacag ggctgtatat tttttatttc
     7861 agattacctt aaatgattcc atttactttt tatctcattt tttcctacta gttagtatca
     7921 aatactctcc tattttctca ataaactatt tttggataca tggtatctga aacagctaaa
     7981 accttgtcat tacagcaaaa aatcacgtta tccagactgt agacaaagtc cattttatgg
     8041 atgatgtcta cagtcttttc ttttataata gatttatgaa aacgaatgca aggagtgata
     8101 gctatgatgt ctaaaaacaa tacgaatggt agaaatcaat ttgcgatgct tacgattgat
     8161 gatctagttc ctcaagatca tctcgttcga aagattgatg ccgcgcttga ttttgaattt
     8221 atttatccaa tcgtcgaagc cacttactcc gatttaggtc gcccaagtat cgatccagtg
     8281 attctgatca aattggtgtt tattcagtat ttattcggta tccgttccat gcgacaaacc
     8341 attaaggaag tcgataccaa tgtcgcttac cgctggtttt taggctactc cttcgaggag
     8401 aaaatccccc ctttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
     8461 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
     8521 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
     8581 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
     8641 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
     8701 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
     8761 gaacgagaaa aacaatttgc ttattctgtc catacttcat gcgacgacaa tggatttatt
     8821 cttagcacaa tcatcactcc tagaaatatt catgacagtc aagtcgcctt tcagctcgtc
     8881 aaacaatcga agagactttt tcctgaaatc aattgtgttg tcgctgatgc tgggtataag
     8941 acacctaagt ttgttcactt tttgactcat ttgaaccttc gaccttgttt gccttattca
     9001 aggcccaaag ggaaaaagga gctattatca aaaaatgagt ttctctatga cgagtatttt
     9061 gattgttata tttgtccgca agatcaaatg ctcgcttttt caacggttac tcgagaaggg
     9121 taccgagaat acaaatccaa tccaaaagaa tgtgtgaact gtcctctgct aaaccaatgt
     9181 acca
//
LOCUS       DANKAY010000103         9171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000103 DANKAY010000000
VERSION     DANKAY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(364..1482)
                     /locus_tag="PWY98_002385"
     CDS             complement(364..1482)
                     /locus_tag="PWY98_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038103.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1953..3260
                     /locus_tag="PWY98_002386"
     CDS             1953..3260
                     /locus_tag="PWY98_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2038104.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="PWY98_002387"
     CDS             3379..4578
                     /locus_tag="PWY98_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2038105.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="PWY98_002388"
     CDS             complement(4605..5873)
                     /locus_tag="PWY98_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2038106.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6036..6682)
                     /locus_tag="PWY98_002389"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="PWY98_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="PWY98_002390"
     CDS             complement(7090..7593)
                     /locus_tag="PWY98_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2038107.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="PWY98_002391"
     CDS             complement(7716..8480)
                     /locus_tag="PWY98_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2038108.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="PWY98_002392"
     CDS             8588..8863
                     /locus_tag="PWY98_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2038109.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         2729 a         1939 c         1635 g         2868 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc t
//
LOCUS       DANKAY010000104         9113 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000104 DANKAY010000000
VERSION     DANKAY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            23..646
                     /locus_tag="PWY98_002393"
     CDS             23..646
                     /locus_tag="PWY98_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038110.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="PWY98_002394"
     CDS             1096..2145
                     /locus_tag="PWY98_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2038111.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="PWY98_002395"
     CDS             2142..3023
                     /locus_tag="PWY98_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038112.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="PWY98_002396"
     CDS             3026..3796
                     /locus_tag="PWY98_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2038113.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="PWY98_002397"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="PWY98_002397"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2038114.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="PWY98_002398"
     CDS             5107..6270
                     /locus_tag="PWY98_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2038115.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="PWY98_002399"
     CDS             6387..6797
                     /locus_tag="PWY98_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2038116.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..>9113
                     /gene="topB"
                     /locus_tag="PWY98_002400"
     CDS             6971..>9113
                     /gene="topB"
                     /locus_tag="PWY98_002400"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2038117.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFS"
BASE COUNT         3140 a         1563 c         1976 g         2434 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt ctt
//
LOCUS       DANKAY010000105         9064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000105 DANKAY010000000
VERSION     DANKAY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            168..362
                     /locus_tag="PWY98_002401"
     CDS             168..362
                     /locus_tag="PWY98_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038118.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="PWY98_002402"
     CDS             631..930
                     /locus_tag="PWY98_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038119.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="PWY98_002403"
     CDS             complement(1027..1461)
                     /locus_tag="PWY98_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2038120.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="PWY98_002404"
     CDS             complement(1693..2403)
                     /locus_tag="PWY98_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2038121.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="PWY98_002405"
     CDS             complement(2393..3253)
                     /locus_tag="PWY98_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2038122.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="PWY98_002406"
     CDS             complement(3258..3896)
                     /locus_tag="PWY98_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2038123.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="PWY98_002407"
     CDS             complement(3911..4210)
                     /locus_tag="PWY98_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2038124.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="PWY98_002408"
     CDS             complement(4253..5737)
                     /locus_tag="PWY98_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2038125.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="PWY98_002409"
     CDS             complement(5757..6218)
                     /locus_tag="PWY98_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2038126.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PWY98_002410"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PWY98_002410"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2038127.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="PWY98_002411"
     CDS             7521..8291
                     /locus_tag="PWY98_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2038128.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..>9064)
                     /locus_tag="PWY98_002412"
     CDS             complement(8564..>9064)
                     /locus_tag="PWY98_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2038129.1"
                     /translation="QILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAAN
                     VAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQL
                     CEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKV
                     IENDLK"
BASE COUNT         2834 a         1864 c         1435 g         2931 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttg
//
LOCUS       DANKAY010000106         8942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000106 DANKAY010000000
VERSION     DANKAY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            51..260
                     /locus_tag="PWY98_002413"
     CDS             51..260
                     /locus_tag="PWY98_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038130.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="PWY98_002414"
                     /pseudo
     CDS             467..667
                     /locus_tag="PWY98_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="PWY98_002415"
     CDS             complement(1379..1984)
                     /locus_tag="PWY98_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2038131.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="PWY98_002416"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="PWY98_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="PWY98_002417"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="PWY98_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="PWY98_002418"
     CDS             3019..3990
                     /locus_tag="PWY98_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2038132.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="PWY98_002419"
     CDS             4130..5443
                     /locus_tag="PWY98_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2038133.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="PWY98_002420"
     CDS             5486..6352
                     /locus_tag="PWY98_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2038134.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="PWY98_002421"
     CDS             6349..7197
                     /locus_tag="PWY98_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2038135.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="PWY98_002422"
     CDS             7211..8683
                     /locus_tag="PWY98_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2038136.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="PWY98_002423"
     CDS             8700..>8942
                     /locus_tag="PWY98_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2038137.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DANKAY010000107         8910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000107 DANKAY010000000
VERSION     DANKAY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            183..2417
                     /gene="pcrA"
                     /locus_tag="PWY98_002424"
     CDS             183..2417
                     /gene="pcrA"
                     /locus_tag="PWY98_002424"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2038138.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2434..4470
                     /gene="ligA"
                     /locus_tag="PWY98_002425"
     CDS             2434..4470
                     /gene="ligA"
                     /locus_tag="PWY98_002425"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2038139.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4576..4881
                     /gene="gatC"
                     /locus_tag="PWY98_002426"
     CDS             4576..4881
                     /gene="gatC"
                     /locus_tag="PWY98_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2038140.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4881..6353
                     /gene="gatA"
                     /locus_tag="PWY98_002427"
     CDS             4881..6353
                     /gene="gatA"
                     /locus_tag="PWY98_002427"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2038141.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6350..7780
                     /gene="gatB"
                     /locus_tag="PWY98_002428"
     CDS             6350..7780
                     /gene="gatB"
                     /locus_tag="PWY98_002428"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2038142.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7790..8845
                     /locus_tag="PWY98_002429"
     CDS             7790..8845
                     /locus_tag="PWY98_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2038143.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
BASE COUNT         3041 a         1628 c         2002 g         2239 t
ORIGIN      
        1 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
       61 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
      121 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
      181 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
      241 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
      301 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
      361 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
      421 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
      481 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
      541 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
      601 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
      661 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
      721 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
      781 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
      841 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
      901 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
      961 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     1021 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     1081 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     1141 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     1201 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     1261 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     1321 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     1381 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     1441 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     1501 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     1561 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     1621 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     1681 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     1741 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     1801 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     1861 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     1921 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     1981 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     2041 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     2101 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     2161 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     2221 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     2281 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     2341 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     2401 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     2461 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     2521 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     2581 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     2641 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     2701 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     2761 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     2821 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     2881 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     2941 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     3001 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     3061 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     3121 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     3181 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     3241 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     3301 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     3361 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     3421 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     3481 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     3541 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     3601 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     3661 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     3721 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     3781 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     3841 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     3901 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     3961 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     4021 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     4081 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     4141 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     4201 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     4261 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     4321 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     4381 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     4441 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     4501 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     4561 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     4621 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     4681 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     4741 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     4801 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     4861 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     4921 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     4981 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     5041 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     5101 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     5161 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     5221 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     5281 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     5341 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     5401 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     5461 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     5521 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     5581 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     5641 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     5701 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     5761 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     5821 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     5881 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     5941 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     6001 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     6061 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     6121 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     6181 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     6241 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     6301 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     6361 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     6421 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     6481 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     6541 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     6601 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     6661 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     6721 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     6781 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     6841 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     6901 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     6961 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     7021 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     7081 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     7141 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     7201 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     7261 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     7321 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     7381 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     7441 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     7501 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     7561 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     7621 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     7681 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     7741 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     7801 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     7861 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     7921 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     7981 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     8041 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     8101 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     8161 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     8221 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     8281 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     8341 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
     8401 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
     8461 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
     8521 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
     8581 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
     8641 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
     8701 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
     8761 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
     8821 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
     8881 cggttttagt tgtgtaatta gctgatttat
//
LOCUS       DANKAY010000108         8828 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000108 DANKAY010000000
VERSION     DANKAY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            35..1375
                     /locus_tag="PWY98_002430"
     CDS             35..1375
                     /locus_tag="PWY98_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2038144.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLTGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1593..2036
                     /locus_tag="PWY98_002431"
     CDS             1593..2036
                     /locus_tag="PWY98_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2038145.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="PWY98_002432"
     CDS             complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="PWY98_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2038146.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4361..5584)
                     /locus_tag="PWY98_002433"
     CDS             complement(4361..5584)
                     /locus_tag="PWY98_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2038147.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5615..6454)
                     /locus_tag="PWY98_002434"
     CDS             complement(5615..6454)
                     /locus_tag="PWY98_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2038148.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6471..7151)
                     /locus_tag="PWY98_002435"
     CDS             complement(6471..7151)
                     /locus_tag="PWY98_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038149.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7153..8226)
                     /locus_tag="PWY98_002436"
     CDS             complement(7153..8226)
                     /locus_tag="PWY98_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2038150.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8282..8520)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8563..8796)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2524 a         1810 c         1611 g         2883 t
ORIGIN      
        1 ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca
       61 acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt
      121 taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat
      181 tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg
      241 cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga
      301 ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc
      361 agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat
      421 gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga
      481 atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta
      541 tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga
      601 ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat
      661 ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa
      721 acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat
      781 tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag
      841 tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt
      901 tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat
      961 tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa
     1021 gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc
     1081 tctttcttat tttgtcggtg acaaattatt tggctatctg acaggttccc tttcgtatac
     1141 agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg
     1201 aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct
     1261 tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt
     1321 catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg
     1381 atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga
     1441 caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg
     1501 gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg acatgataga
     1561 agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
     1621 tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg
     1681 atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg
     1741 cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga
     1801 tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta
     1861 ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg
     1921 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
     1981 aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
     2041 tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag
     2101 aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa
     2161 taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc
     2221 agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag
     2281 tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc
     2341 gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc
     2401 gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac
     2461 cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc
     2521 acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg
     2581 gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag
     2641 gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac
     2701 ttgctacgaa acttccggca attccccaag gcactaagta gttgattact gttccaccat
     2761 cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa
     2821 atgcattacc aggcagaata acagatagga attgttcacc aataaaaata tttactccga
     2881 tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc
     2941 gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta
     3001 gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat
     3061 cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg
     3121 ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca
     3181 aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa
     3241 tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag
     3301 atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa
     3361 caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa
     3421 caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg
     3481 cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac
     3541 cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta
     3601 cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc
     3661 caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga
     3721 ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt
     3781 gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg
     3841 ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa
     3901 caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga
     3961 taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc
     4021 gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg
     4081 acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt
     4141 ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg
     4201 accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga
     4261 agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga
     4321 aaattatttt tataactttc acacacaaga ttatttttta ctagaaagta gcgccctctt
     4381 gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct
     4441 ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa
     4501 ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct
     4561 cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag
     4621 aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt
     4681 ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat
     4741 tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat
     4801 tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac
     4861 gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat
     4921 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga
     4981 tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac
     5041 cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa
     5101 aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc
     5161 tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt
     5221 ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac
     5281 caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa
     5341 aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc
     5401 cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg
     5461 aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt
     5521 gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg
     5581 tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg
     5641 caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat
     5701 aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga
     5761 tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag
     5821 gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca
     5881 aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg
     5941 taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag
     6001 tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg
     6061 ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat
     6121 tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa
     6181 agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt
     6241 gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt
     6301 tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt
     6361 ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa
     6421 atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta
     6481 atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat
     6541 aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga
     6601 ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg
     6661 atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa
     6721 atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact
     6781 tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc
     6841 actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa
     6901 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt
     6961 tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct
     7021 aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc
     7081 ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag
     7141 aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt
     7201 ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg
     7261 tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc
     7321 tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag
     7381 aggttcgttc gtctgatttc caatataact taattccacc agccatttat tggcgatctg
     7441 atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt
     7501 tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc
     7561 tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac
     7621 ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa
     7681 caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa
     7741 taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc
     7801 cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc
     7861 attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac
     7921 gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc
     7981 ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat
     8041 gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc
     8101 tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga
     8161 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc
     8221 aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt
     8281 tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact
     8341 cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat
     8401 ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc
     8461 agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa
     8521 tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg
     8581 cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat
     8641 tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct
     8701 ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa
     8761 agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta
     8821 catgagga
//
LOCUS       DANKAY010000109         8674 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000109 DANKAY010000000
VERSION     DANKAY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(49..495)
                     /locus_tag="PWY98_002437"
     CDS             complement(49..495)
                     /locus_tag="PWY98_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2038151.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(522..698)
                     /locus_tag="PWY98_002438"
     CDS             complement(522..698)
                     /locus_tag="PWY98_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2038152.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(860..1288)
                     /locus_tag="PWY98_002439"
     CDS             complement(860..1288)
                     /locus_tag="PWY98_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2038153.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1380..2240
                     /locus_tag="PWY98_002440"
     CDS             1380..2240
                     /locus_tag="PWY98_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2038154.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(2334..2684)
                     /locus_tag="PWY98_002441"
     CDS             complement(2334..2684)
                     /locus_tag="PWY98_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2038155.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2700..4427)
                     /locus_tag="PWY98_002442"
     CDS             complement(2700..4427)
                     /locus_tag="PWY98_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2038156.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="PWY98_002443"
     CDS             complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="PWY98_002443"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2038157.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5711..7147)
                     /locus_tag="PWY98_002444"
     CDS             complement(5711..7147)
                     /locus_tag="PWY98_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2038158.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7144..8547)
                     /locus_tag="PWY98_002445"
     CDS             complement(7144..8547)
                     /locus_tag="PWY98_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2038159.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         2340 a         1946 c         1491 g         2897 t
ORIGIN      
        1 gaatttcttc accaacactc attgacgaag agcctttaaa tgataaaatt aagattgtaa
       61 tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat
      121 gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc
      181 ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac
      241 tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc
      301 atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc
      361 tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat
      421 gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct
      481 acttagtagt gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt
      541 ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta
      601 cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg
      661 tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct
      721 taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat
      781 attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa
      841 tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat
      901 gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga
      961 tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt
     1021 gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga
     1081 tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct
     1141 gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag
     1201 ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg
     1261 attgttcaat caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa
     1321 gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca
     1381 tgtcttccga ttctacagaa atcattactt tttttgtcat ctctgactct gctggagaaa
     1441 ctgcgacaaa attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat
     1501 tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa
     1561 aagaaaaagc ccttgtccta cacacattga tcaatcaaga attgatcgag atgacgcgtt
     1621 ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa
     1681 tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg
     1741 agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag
     1801 atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa
     1861 cgcctttgag cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc
     1921 cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga
     1981 cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa
     2041 atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg
     2101 atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa
     2161 cagcttcact tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa
     2221 ccaccgaaga aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt
     2281 cagcgaagaa gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga
     2341 tgttccacat gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
     2401 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
     2461 agttgatgcg atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt
     2521 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
     2581 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
     2641 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
     2701 tattcttctg tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct
     2761 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
     2821 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
     2881 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
     2941 tggatgactt ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa
     3001 gcagagattt ttgcagagcc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
     3061 tagtagccat atgtttcaaa aatcgtttgc aagccgtcat acaatgtttt gccttgtttt
     3121 ttatagtaag ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga
     3181 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
     3241 tgatcttctt caaattgttg aattttttca gcaatgaatt tgaatcctgt caatacattg
     3301 aacatggtag ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat
     3361 ttcaataccg ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
     3421 tgagcaaaca gggctccgat ttggtttcct gtcaatactt cataggagcc atttggcaaa
     3481 cgaacggctg cgcctaaacg gtctgcatca ggatccgttg caatcagtaa gtcagcgcct
     3541 tctttctcgc caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat
     3601 ttgactgtag tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
     3661 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
     3721 tacacaagct tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct
     3781 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
     3841 tgttcagctt cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
     3901 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
     3961 ttatatccgt tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc
     4021 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa
     4081 gaagggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
     4141 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc
     4201 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
     4261 ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
     4321 tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa gttttcttct
     4381 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
     4441 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtatgag
     4501 gaccaaaaaa cgcttaaatc aagaaaagat tcttttatca agcacggtta caagtattcg
     4561 cataaatcgg attcagtaga tttcacgcaa aaaaaagagt aaaagaacaa ttgaaaccgt
     4621 tctttcactc tttttaaact tatgcagttg tttcttctaa agattccaag tatttataca
     4681 cttgttgtcc agcaatccca ccatcaccaa cagcagtagt gatttgtcgc aaatcttttt
     4741 cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg ttgtctgttg
     4801 gaatccagcc ggcttcgttc gtcaacccag cttctctaaa tggtgcagtt aatggatcca
     4861 atcctacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgcca gttttgacat
     4921 tacgtgcttt cacacctgtc actaccattt catttccaac gatttcgtca acgactgtat
     4981 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
     5041 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
     5101 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaatg
     5161 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
     5221 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
     5281 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
     5341 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
     5401 ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc atttggccgc
     5461 caggagcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
     5521 cagtcatccc agcaggccct gcgccaataa cgattacatc atacatatgt agattctcct
     5581 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
     5641 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
     5701 aaatcattga tcaatcgttc gaaagatttt ctccatttga atcaattact tttttatacc
     5761 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
     5821 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
     5881 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
     5941 cctggatatg tgctgccaga tagtcgattc gataatcatc ttccacataa ccattctcat
     6001 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
     6061 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
     6121 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
     6181 gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata aaatcaacag
     6241 tatgttcttt cagcaatgcg gcatccccag gttccatttt gatctgaatg ccatttcgtt
     6301 ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca atgaagaaat
     6361 aattttctcg atctgacttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
     6421 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcga
     6481 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
     6541 cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca tgaaggatca
     6601 tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga ttgaagatga
     6661 ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
     6721 tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg atcccgtact
     6781 tttgacattc tttaaagaca tcctcataaa atttcaaccc agattcattt ggctcttttt
     6841 catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc ttgaacccca
     6901 tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc gcaatttttg
     6961 ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta atgacaggaa
     7021 aacggtcttc tccgattggc acaacatcta cgttcgctaa cccacgtccg ccttcatcat
     7081 aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct tttctgaatc
     7141 ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca gttcatcttg
     7201 gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg gtaaatactg
     7261 ctgatgtgca ataaacaact catccatgat ccgttttgct gtctttcctg atcgtataag
     7321 cggattgatc gtaaacgctt gcaaagctgt tccataatca ccagtcacgg ctgcttcgat
     7381 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaaggga
     7441 accaaacgct acatttcgtg cgccttgtgc gccaacatag gcagtgactt ctactacaca
     7501 atctgccggc agatcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
     7561 tttatttcca tagatcgaag caatcgtttc acaggcagca tctgaataat aagcaccacc
     7621 tcgtttttgc aattgttcag gtttatgatc caattctgga tctttgtata attcaaacag
     7681 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
     7741 agcatgttcc agcatttctt cttcacgata gtaatagcgg tggtagccac aagggatcat
     7801 gttcatttgt tttaattgtt ctttgaaaaa cgggatgtca aagatatttt tcggcaatcc
     7861 attgtcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
     7921 tgctactttg tgccaatgga agtgatttag accggcaaac ttgtagatca gctcttcttt
     7981 tttcttgcca agcattgttg gttctgtcat catcgccatt actggcacat tgcataatcc
     8041 gatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cgcttgggtt
     8101 cgcgaaatta atcagccaag catttggaca taaacgcttc atgtcttcaa cgattgccaa
     8161 aaccactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
     8221 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
     8281 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
     8341 atagattttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactgacgat
     8401 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
     8461 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
     8521 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttac acttttatta
     8581 tcttttttgt aaacaaaaca aacaatagaa attcgtttct ttccagtaac atttacaaaa
     8641 gttagtgttt tcttctggat ttttgtaaat atct
//
LOCUS       DANKAY010000110         8494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000110 DANKAY010000000
VERSION     DANKAY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..173)
                     /locus_tag="PWY98_002446"
     CDS             complement(<1..173)
                     /locus_tag="PWY98_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2038160.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWY98_002447"
     CDS             complement(593..1714)
                     /locus_tag="PWY98_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL2038161.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWY98_002448"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWY98_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2038162.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWY98_002449"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWY98_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2038163.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWY98_002450"
     CDS             complement(4304..5215)
                     /locus_tag="PWY98_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2038164.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWY98_002451"
     CDS             complement(5232..6239)
                     /locus_tag="PWY98_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2038165.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWY98_002452"
     CDS             complement(6236..8365)
                     /locus_tag="PWY98_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2038166.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="PWY98_002453"
     CDS             complement(8370..>8494)
                     /locus_tag="PWY98_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2038167.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DANKAY010000111         8482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000111 DANKAY010000000
VERSION     DANKAY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            20..862
                     /locus_tag="PWY98_002454"
     CDS             20..862
                     /locus_tag="PWY98_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2038168.1"
                     /translation="MLLFFEITSVASLITEVKGQTHSSEHSSQSFKQSEQSTIKSQST
                     ETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFDDGPGATTTPQLLRI
                     LKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNLATLSAQSMTEEVTK
                     ADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSEDWKTRNPDLILQKIQ
                     ATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLGHPTAVENYYGRNDH
                     RPVQ"
     gene            complement(1054..1125)
                     /locus_tag="PWY98_002455"
     tRNA            complement(1054..1125)
                     /locus_tag="PWY98_002455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1090..1092),aa:Arg,seq:ccg)
     gene            1185..2519
                     /gene="rpoN"
                     /locus_tag="PWY98_002456"
     CDS             1185..2519
                     /gene="rpoN"
                     /locus_tag="PWY98_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2038169.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            2668..3759
                     /locus_tag="PWY98_002457"
     CDS             2668..3759
                     /locus_tag="PWY98_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2038170.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            3800..4801
                     /gene="gap"
                     /locus_tag="PWY98_002458"
     CDS             3800..4801
                     /gene="gap"
                     /locus_tag="PWY98_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2038171.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            4911..6101
                     /locus_tag="PWY98_002459"
     CDS             4911..6101
                     /locus_tag="PWY98_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2038172.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            6205..6960
                     /locus_tag="PWY98_002460"
     CDS             6205..6960
                     /locus_tag="PWY98_002460"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2038173.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            7076..8374
                     /gene="eno"
                     /locus_tag="PWY98_002461"
     CDS             7076..8374
                     /gene="eno"
                     /locus_tag="PWY98_002461"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2038174.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         2921 a         1601 c         1782 g         2178 t
ORIGIN      
        1 cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat
       61 caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc
      121 agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca
      181 agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca gtacaaaaac
      241 gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac
      301 cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt
      361 cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca
      421 cgagattggc aatcacacat acgatcatca gaatctggcc actctttctg cccagtcgat
      481 gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca aaacccccac
      541 ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca tccagcgacc
      601 gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg atttgatttt
      661 gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg atatctatcc
      721 tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag ggtatcgcat
      781 cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt atggcagaaa
      841 cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca cattttaata
      901 agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat acaggtacaa
      961 caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat aaagaagcaa
     1021 ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc gaacccccgt
     1081 ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca ctgggataat
     1141 tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa tttgagcaaa
     1201 atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag ttgcaacagt
     1261 ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag aatcaagcaa
     1321 tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac ataaaagctt
     1381 cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct gatacaaaag
     1441 ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat acttttttga
     1501 ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg acgattagtt
     1561 tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat gcgttgactc
     1621 tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa tgtctcatgc
     1681 ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg gaagaatgtt
     1741 ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct gttgatctac
     1801 atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct ggccggattt
     1861 tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg gacgacgata
     1921 aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt caagagagtt
     1981 acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta aaagaacgga
     2041 aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca attttgcggg
     2101 ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa cgtccaatca
     2161 agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc acggtcagtc
     2221 gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt atttgaatta aaaaaatttt
     2281 tcactacacg aattcctacg aatagtacag aacagacaga agacctttca gcagatacag
     2341 cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg ttgtctgacc
     2401 agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga acagtagcaa
     2461 aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat gataactaat
     2521 cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg aaaaacgaga
     2581 gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg
     2641 aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt ggacgtaaag
     2701 tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc tgttgcacca
     2761 gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta ttggatgcag
     2821 ccgattggac gtcgaagttt atcggaaagt atggggatca cagaacgagt attgcgaaca
     2881 gaaacagatc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg tatgacgctg
     2941 acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca gcttttaggt
     3001 acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg tatcgttgtt
     3061 tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa
     3121 gccctagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg aggaactact
     3181 atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca taacctgttt
     3241 gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc tgtcagtgca
     3301 agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt atgttccgga acaattaagt
     3361 ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt aaacttgatt
     3421 agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc agcacgtcgt
     3481 aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc ggaatctttt
     3541 ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat cggcatagaa
     3601 ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa atcaaaagct
     3661 aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa
     3721 gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt ttttgatttt
     3781 cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg gacgtattgg
     3841 acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag caatcaacga
     3901 cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc aaggacgttt
     3961 caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag aagttaaagt
     4021 attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag atatcgttct
     4081 agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa cagctggtgc
     4141 taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg tttataacac
     4201 aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat gtactactaa
     4261 ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg aaggattaat
     4321 gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc atcctaaagg
     4381 tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc
     4441 taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa ttagacggag ctgctcaacg
     4501 tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa aagaagtaac
     4561 tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg gatacaacac
     4621 agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat ttgatgctac
     4681 acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg cttggtacga
     4741 caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg ctaacttata
     4801 agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg gggaagcaat
     4861 cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa atggctaaga
     4921 aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg
     4981 ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca ttgccaacaa
     5041 tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa
     5101 aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat
     5161 tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa
     5221 tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt gaagatattg
     5281 acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct tcattaggcg
     5341 atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct aacgtaggta
     5401 tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa atcaagttca
     5461 tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt ggcgcaaaag
     5521 tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa atcttgatcg
     5581 gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt aactctctag
     5641 tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc gacaaacttg
     5701 tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa
     5761 tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca tcagtaaaat
     5821 tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat
     5881 ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag
     5941 aaggcgcaac aacaatcatc ggtggcggag actcagctgc agcagcagaa caattaggat
     6001 ttgctgacaa attcacacac atctcaacag gtggtggcgc aagcttggaa ttgcttgaag
     6061 gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag tttgctcaat
     6121 tctgacgagt aatcgatcaa aaggtgaagg gagttttcat gactccctag ccatttttca
     6181 ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg
     6241 aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa aatcccttca
     6301 aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga attaatgaca
     6361 tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga aaactcaggt
     6421 gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca ttatgtgatc
     6481 atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat caataaaaaa
     6541 gcaaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgcggcga atctttagaa
     6601 acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc tggtttagct
     6661 gatctttcag ctgaacaagt gtcaaacatg gttattgctt atgaaccaat ctgggcaatc
     6721 ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa
     6781 acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca atacggcggt
     6841 tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga cggtgcttta
     6901 gtcggtggcg caagcttgca agcagattca tttttgtcat tgttagacgc tgtaaaataa
     6961 ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa attaactaaa
     7021 atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac aaaacatgtc
     7081 aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc caacaatcga
     7141 agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt caggtgcttc
     7201 aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt atggcggtaa
     7261 aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa tcattggcta
     7321 cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg gaactcctaa
     7381 caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg cacgtgcagc
     7441 tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca caaaagtatt
     7501 gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact caatcgactt
     7561 ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc tacgtatggg
     7621 tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag caacttctgt
     7681 tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat
     7741 catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc ttgctatgga
     7801 tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag ctgactcagg
     7861 cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag tttctaaata
     7921 tccaatcatc tcaatcgaag atggattaga cgaaaacgac tgggacggat tcaagaaatt
     7981 aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg taacaaacac
     8041 tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa
     8101 ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag aagctggcta
     8161 cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct ctgatatcgc
     8221 tgttgcaaca aatgctggcc aaatcaaaac tggttctcta tcacgtactg accgtattgc
     8281 taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag aatacaaagg
     8341 tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag atacatgatc
     8401 gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc tctacttgct
     8461 gaaatatcag caggtagaga gt
//
LOCUS       DANKAY010000112         8453 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000112 DANKAY010000000
VERSION     DANKAY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..273
                     /locus_tag="PWY98_002462"
     CDS             <1..273
                     /locus_tag="PWY98_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038175.1"
                     /translation="LLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQFIFLFE
                     HGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            689..2227
                     /locus_tag="PWY98_002463"
     CDS             689..2227
                     /locus_tag="PWY98_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2038176.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2224..3189
                     /locus_tag="PWY98_002464"
     CDS             2224..3189
                     /locus_tag="PWY98_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2038177.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3176..4498
                     /locus_tag="PWY98_002465"
     CDS             3176..4498
                     /locus_tag="PWY98_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2038178.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4561..4665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4739..5482
                     /locus_tag="PWY98_002466"
     CDS             4739..5482
                     /locus_tag="PWY98_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038179.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5497..6489
                     /locus_tag="PWY98_002467"
     CDS             5497..6489
                     /locus_tag="PWY98_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2038180.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6501..7238
                     /locus_tag="PWY98_002468"
     CDS             6501..7238
                     /locus_tag="PWY98_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2038181.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7228..7950
                     /locus_tag="PWY98_002469"
     CDS             7228..7950
                     /locus_tag="PWY98_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2038182.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8034..>8453)
                     /locus_tag="PWY98_002470"
     CDS             complement(8034..>8453)
                     /locus_tag="PWY98_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2038183.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2727 a         1456 c         1822 g         2448 t
ORIGIN      
        1 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
       61 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
      121 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
      181 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
      241 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
      301 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
      361 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
      421 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
      481 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
      541 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
      601 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
      661 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
      721 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
      781 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
      841 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
      901 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
      961 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     1021 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     1081 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     1141 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     1201 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     1261 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     1321 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     1381 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     1441 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     1501 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     1561 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     1621 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     1681 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     1741 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     1801 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     1861 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     1921 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     1981 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     2041 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     2101 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     2161 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     2221 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     2281 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     2341 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     2401 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     2461 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     2521 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     2581 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     2641 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     2701 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     2761 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     2821 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     2881 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     2941 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     3001 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     3061 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     3121 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     3181 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     3241 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     3301 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     3361 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     3421 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     3481 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     3541 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     3601 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     3661 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     3721 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     3781 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     3841 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     3901 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     3961 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     4021 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     4081 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     4141 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     4201 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     4261 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     4321 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     4381 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     4441 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     4501 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     4561 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     4621 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     4681 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     4741 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     4801 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     4861 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     4921 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     4981 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
     5041 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
     5101 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
     5161 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
     5221 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
     5281 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
     5341 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
     5401 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
     5461 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
     5521 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
     5581 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
     5641 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
     5701 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
     5761 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
     5821 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
     5881 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
     5941 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
     6001 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
     6061 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
     6121 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
     6181 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
     6241 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
     6301 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
     6361 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
     6421 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
     6481 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
     6541 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
     6601 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
     6661 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
     6721 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
     6781 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
     6841 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
     6901 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
     6961 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
     7021 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
     7081 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
     7141 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
     7201 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
     7261 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
     7321 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
     7381 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
     7441 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
     7501 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
     7561 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
     7621 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
     7681 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
     7741 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
     7801 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
     7861 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
     7921 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
     7981 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
     8041 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
     8101 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
     8161 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
     8221 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
     8281 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
     8341 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
     8401 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DANKAY010000113         8400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000113 DANKAY010000000
VERSION     DANKAY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            8..730
                     /locus_tag="PWY98_002471"
     CDS             8..730
                     /locus_tag="PWY98_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2038184.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="PWY98_002472"
     CDS             788..2827
                     /locus_tag="PWY98_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2038185.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="PWY98_002473"
     CDS             complement(2936..4087)
                     /locus_tag="PWY98_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2038186.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="PWY98_002474"
     CDS             complement(4241..4771)
                     /locus_tag="PWY98_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2038187.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="PWY98_002475"
     CDS             complement(4870..5220)
                     /locus_tag="PWY98_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2038188.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="PWY98_002476"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="PWY98_002476"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2038189.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="PWY98_002477"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="PWY98_002477"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2038190.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="PWY98_002478"
     CDS             complement(7623..8285)
                     /locus_tag="PWY98_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2038191.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT         2835 a         1618 c         1526 g         2421 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
//
LOCUS       DANKAY010000114         8335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000114 DANKAY010000000
VERSION     DANKAY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..1336)
                     /gene="celB"
                     /locus_tag="PWY98_002479"
     CDS             complement(<1..1336)
                     /gene="celB"
                     /locus_tag="PWY98_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2038192.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELA"
     gene            complement(1336..1854)
                     /locus_tag="PWY98_002480"
     CDS             complement(1336..1854)
                     /locus_tag="PWY98_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038193.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1870..2196)
                     /locus_tag="PWY98_002481"
     CDS             complement(1870..2196)
                     /locus_tag="PWY98_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2038194.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2209..4188)
                     /locus_tag="PWY98_002482"
     CDS             complement(2209..4188)
                     /locus_tag="PWY98_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2038195.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4209..4523)
                     /locus_tag="PWY98_002483"
     CDS             complement(4209..4523)
                     /locus_tag="PWY98_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2038196.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4691..4984
                     /locus_tag="PWY98_002484"
     CDS             4691..4984
                     /locus_tag="PWY98_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2038197.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5062..6174)
                     /locus_tag="PWY98_002485"
     CDS             complement(5062..6174)
                     /locus_tag="PWY98_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2038198.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6561..6740
                     /locus_tag="PWY98_002486"
     CDS             6561..6740
                     /locus_tag="PWY98_002486"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038199.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6934..7104
                     /locus_tag="PWY98_002487"
     CDS             6934..7104
                     /locus_tag="PWY98_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2038200.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7528..8268)
                     /locus_tag="PWY98_002488"
     CDS             complement(7528..8268)
                     /locus_tag="PWY98_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2038201.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2596 a         1715 c         1370 g         2654 t
ORIGIN      
        1 tagctaattc tgctgccttt tctctttctt catttaataa tttattatca tagaatttga
       61 taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac acagcagctt
      121 tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc cacggcatct
      181 gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt gtagcagaag
      241 ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt ggcatcccga
      301 aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt cctaacactt
      361 tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa gtcgcacctg
      421 atccaccaac ggttacataa gaattgttga attctcctgc caacggataa tttgcgcctt
      481 gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg atgatattcg
      541 ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc atgacaccaa
      601 gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt ttgaagatat
      661 ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca atgacaaatg
      721 ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct ggcattttta
      781 tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc gacataataa
      841 tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc ctaataccat
      901 ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca tcagtcatga
      961 tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc gtgagcggat
     1021 taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca aacacaatgg
     1081 caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac aagtcactaa
     1141 ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat
     1201 ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc cccgctgcca
     1261 tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc aggtatttct
     1321 gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt catattgttt
     1381 aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat actgtataaa
     1441 tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc ctccattgtt
     1501 atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg gaatcactaa
     1561 taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt tcgtttggtg
     1621 ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa tcagaatcat
     1681 cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt gcatgttcat
     1741 gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat acatgttttt
     1801 caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct gcacaatgat
     1861 ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa cttccaataa
     1921 tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca tttctatttt
     1981 tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat gaagaatctc
     2041 ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac gcatcgagcg
     2101 aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat caaaagaagt
     2161 cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt attcgatcag
     2221 tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt cctgctgtac
     2281 ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc cttcattttc
     2341 gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat cccactgcat
     2401 cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa cagcgggatg
     2461 tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt gctggatctg
     2521 ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt gcagcaaatc
     2581 atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa
     2641 ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg gtctatctga
     2701 ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat catcttcatt
     2761 caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa gatcaataga
     2821 agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat agccttttac
     2881 acttaccact gtaatatttt taccgaattc actgactacg cgattcttta atagttgggc
     2941 actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag ctctttcttt
     3001 ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat tgatggtata
     3061 gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga ctttgggatt
     3121 ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat
     3181 catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct ctactaatga
     3241 aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg atagttcctg
     3301 atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca aataagcaat
     3361 ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga tccgctgcgc
     3421 cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct tgatttcaaa
     3481 cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct gtatgataaa
     3541 atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga ctagctcttc
     3601 aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg aatttccata
     3661 atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt ccccttcgat
     3721 ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca atcgctcctt
     3781 gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt ctgccaaatc
     3841 atccataaaa agaatggcat cttccaaaag caattggttc aatatatact tttgtcgatc
     3901 ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt cttgccgact
     3961 caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat aacctggttt
     4021 agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca agtaattacg
     4081 gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga catactcctt
     4141 cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta tcatccactc
     4201 ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc tgtagggatt
     4261 gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc tcctaatttt
     4321 ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc aaaatcacta
     4381 ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac ctcttctctt
     4441 ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat tcctgccgca
     4501 caaataatca atgctttttt cataaatcaa tcactccttt tatttttata aaccaagcat
     4561 atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga aaaaatgtgt
     4621 atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa ttttttcagg
     4681 aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg gacaagccag
     4741 aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg
     4801 aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt tactgatcga
     4861 ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat
     4921 aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca ctgatttacc
     4981 gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt cactttttcc
     5041 ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta tttttcggat
     5101 aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga tattctcaaa
     5161 aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg ccaaagcacc
     5221 aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag ctagaccacc
     5281 taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata aaacacatcc
     5341 aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg acatcaatcc
     5401 atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac
     5461 atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca tccaaatttt
     5521 ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt
     5581 ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa ataataagct
     5641 acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa
     5701 gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag cggttgccat
     5761 taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc atttgatcag
     5821 ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta tgatcgaatg
     5881 ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga ataaaaccac
     5941 agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa aacaaatggc
     6001 aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc acttttcttt
     6061 tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt gtagagactg
     6121 ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag tcatctatga
     6181 caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca actatttttc
     6241 ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc accacaaaat
     6301 aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt ctgagatcaa
     6361 tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag aaaaaaacat
     6421 tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt ggttctgctg
     6481 tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc
     6541 actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag cgctatacct
     6601 gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca aggcactcga
     6661 tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg tcgttatttt
     6721 ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg aaagagttga
     6781 gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt
     6841 tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg gcgagacaga
     6901 aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa ctatttcctt
     6961 agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc cacctacttt
     7021 cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt acttggtttt
     7081 tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct ttccgagaca
     7141 aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag agaaaaaatt
     7201 acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat aaacttgagt
     7261 cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt tgtatatttt
     7321 ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc acaaaattgt
     7381 ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata tttgtttgta
     7441 taccccttct aaaatcggta gtttccattc aattgatggg tactagttga ttttaaaaac
     7501 tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa attttatact
     7561 gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga tggtttctcc
     7621 agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta tatctgagct
     7681 atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt ctttttcgat
     7741 cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag tttctaccag
     7801 ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc ctgtggttag
     7861 acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc cttgcaggac
     7921 ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa gtcgattttc
     7981 tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga ttggtgtcgc
     8041 atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc ctggtgctaa
     8101 gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca gaacggttcc
     8161 attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt ggtcatcgat
     8221 ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt ctaccaccgc
     8281 tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaattcat acccc
//
LOCUS       DANKAY010000115         8248 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000115 DANKAY010000000
VERSION     DANKAY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            234..1559
                     /locus_tag="PWY98_002489"
     CDS             234..1559
                     /locus_tag="PWY98_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2038202.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="PWY98_002490"
     CDS             1552..1902
                     /locus_tag="PWY98_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038203.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="PWY98_002491"
     CDS             1899..2069
                     /locus_tag="PWY98_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038204.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="PWY98_002492"
     CDS             complement(2973..3248)
                     /locus_tag="PWY98_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2038205.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="PWY98_002493"
     CDS             complement(3220..4173)
                     /locus_tag="PWY98_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2038206.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="PWY98_002494"
     CDS             4785..6278
                     /locus_tag="PWY98_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2038207.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="PWY98_002495"
     CDS             6413..6709
                     /locus_tag="PWY98_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2038208.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="PWY98_002496"
     CDS             6810..7661
                     /locus_tag="PWY98_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038209.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="PWY98_002497"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="PWY98_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DANKAY010000116         8124 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000116 DANKAY010000000
VERSION     DANKAY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            78..2687
                     /gene="clpB"
                     /locus_tag="PWY98_002498"
     CDS             78..2687
                     /gene="clpB"
                     /locus_tag="PWY98_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2038210.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2838..3524)
                     /locus_tag="PWY98_002499"
     CDS             complement(2838..3524)
                     /locus_tag="PWY98_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038211.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3502..3996)
                     /locus_tag="PWY98_002500"
     CDS             complement(3502..3996)
                     /locus_tag="PWY98_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2038212.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4016..5236)
                     /locus_tag="PWY98_002501"
     CDS             complement(4016..5236)
                     /locus_tag="PWY98_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2038213.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5383..7218
                     /gene="lepA"
                     /locus_tag="PWY98_002502"
     CDS             5383..7218
                     /gene="lepA"
                     /locus_tag="PWY98_002502"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2038214.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7414..>8124
                     /locus_tag="PWY98_002503"
     CDS             7414..>8124
                     /locus_tag="PWY98_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038215.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSF"
BASE COUNT         2749 a         1480 c         1644 g         2251 t
ORIGIN      
        1 gaccaaagcg atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat
       61 taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg
      121 ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat
      181 ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg
      241 atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg
      301 gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag
      361 cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag
      421 cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg
      481 aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa
      541 accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca
      601 aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta
      661 ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa
      721 cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa
      781 aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg
      841 gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta
      901 tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta
      961 tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg
     1021 ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct
     1081 ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat
     1141 taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag
     1201 cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt
     1261 tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag
     1321 atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat
     1381 cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg
     1441 aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt
     1501 cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact
     1561 atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat
     1621 tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta
     1681 ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg
     1741 tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg
     1801 gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc
     1861 aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa
     1921 ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga
     1981 tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag
     2041 gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata
     2101 caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac
     2161 aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata
     2221 cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg
     2281 atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca
     2341 aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca
     2401 acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag
     2461 aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac
     2521 cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag
     2581 ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg
     2641 atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga
     2701 ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac
     2761 agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc
     2821 tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa
     2881 cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta
     2941 tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca
     3001 tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag
     3061 aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa
     3121 ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga
     3181 gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg
     3241 atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa
     3301 acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc
     3361 aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt
     3421 tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct
     3481 atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac
     3541 ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat
     3601 tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt
     3661 tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc
     3721 tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag
     3781 taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg
     3841 actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga
     3901 aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact
     3961 tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa
     4021 aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt
     4081 cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca
     4141 tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga
     4201 cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga
     4261 caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa
     4321 ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct
     4381 gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt
     4441 agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa
     4501 gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc
     4561 ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac
     4621 gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa
     4681 atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac
     4741 ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca
     4801 tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc
     4861 attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat
     4921 ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac
     4981 cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga
     5041 accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt
     5101 tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca
     5161 attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa
     5221 aatctttttg atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt
     5281 ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt
     5341 ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg
     5401 aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa
     5461 tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa
     5521 gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat
     5581 gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac
     5641 accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt
     5701 gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat
     5761 ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca
     5821 gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa
     5881 gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg
     5941 gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac
     6001 tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc
     6061 aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc
     6121 ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat
     6181 atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca
     6241 gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt
     6301 ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg
     6361 caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg
     6421 ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg
     6481 gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg
     6541 gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag
     6601 gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca
     6661 gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat
     6721 taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac
     6781 aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa
     6841 aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc
     6901 tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa
     6961 aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc
     7021 gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga
     7081 gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa
     7141 caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggatgag
     7201 caagatagtg tgaaataact tataatcaat cgaaaaacct attaaataaa cctttttgag
     7261 tagatgcaac actgaggatt atctgtataa aagattttaa actgtcattt ggttatcacg
     7321 gcgatactaa aattaacaaa attggaatat ttttcttgaa gatttttagc tgtcagtata
     7381 tgatgaaagc atctcatagg aggtgctttt ttcatggttg ataattcaag tggaagggtt
     7441 cttaaatctc taagaaaaga gaaaaaacta tctcaaaaaa agttagctga tttagcaggt
     7501 atttcacaaa gtactttagt taaatatgaa aaagggtcaa gaaaaatccc taaagacgta
     7561 gataatactc taagcaagat tctgaatata gaaactctaa taaaagatga ggaagataag
     7621 attgagattc tcatcggtaa actaattgca tacagagata tgaataagtt acttaacaaa
     7681 gagttagctg ataatatagg aattagtgaa gtattactca gttatgtgtt aaatagaaag
     7741 agaaatccgt caaaagaaat gcaaaaaaaa atcgatattt ttctattaag taatgaaaaa
     7801 gaaattttga aggagattaa tcgtgatagt gagatattta gtttatcaaa agacgataaa
     7861 attgttatgg gaaaaagaat acgagaagtc cgaaagaacc gggaagagac gcttgaaaaa
     7921 tttggtaaaa actttaccat ctatacaggc aaaaatgtta taagtagatg ggaaaaaggt
     7981 attaatattc cagatattga aaaactaatg aatattgcct atcttggtaa ggtcactgtt
     8041 ccttacctta tgtacggaga agattataag aatatacttc caaaagatga aagagttagc
     8101 gattttaaaa agataaattc tttt
//
LOCUS       DANKAY010000117         8004 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000117 DANKAY010000000
VERSION     DANKAY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..551)
                     /locus_tag="PWY98_002504"
     CDS             complement(<1..551)
                     /locus_tag="PWY98_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2038216.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEF"
     gene            complement(553..1317)
                     /locus_tag="PWY98_002505"
     CDS             complement(553..1317)
                     /locus_tag="PWY98_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2038217.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1325..2002)
                     /locus_tag="PWY98_002506"
     CDS             complement(1325..2002)
                     /locus_tag="PWY98_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2038218.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2039..3424)
                     /locus_tag="PWY98_002507"
     CDS             complement(2039..3424)
                     /locus_tag="PWY98_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2038219.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3634..3718)
                     /gene="ffs"
                     /locus_tag="PWY98_002508"
     ncRNA           complement(3634..3718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWY98_002508"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3741..4256)
                     /gene="tadA"
                     /locus_tag="PWY98_002509"
     CDS             complement(3741..4256)
                     /gene="tadA"
                     /locus_tag="PWY98_002509"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2038220.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4363..5220
                     /locus_tag="PWY98_002510"
     CDS             4363..5220
                     /locus_tag="PWY98_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038221.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5301..5636)
                     /locus_tag="PWY98_002511"
     CDS             complement(5301..5636)
                     /locus_tag="PWY98_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038222.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5703..7073)
                     /locus_tag="PWY98_002512"
     CDS             complement(5703..7073)
                     /locus_tag="PWY98_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2038223.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7151..7687)
                     /locus_tag="PWY98_002513"
     CDS             complement(7151..7687)
                     /locus_tag="PWY98_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2038224.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2313 a         1694 c         1287 g         2710 t
ORIGIN      
        1 aaaaattctt ctggaagtac attgatgatt ggatcagtaa cgggatcgaa taaccagtca
       61 tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc tatcatcatg
      121 aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa acggacgaat caaccgccaa
      181 agccgatcat tcttttgcaa atatttgata aacaaaatac tcgggatcaa agtgatgaaa
      241 aacacagcat aatccactaa aaacaaaatt ttcacttcat aaaagtgatg ggctccgcta
      301 gcagaaactg ggaaatctgg caagctgagg atggttttga agggattatt gagataattc
      361 attaggtgat caaaattttt cagtaacgtc tcttgatcta aagaagtata atccaaaatt
      421 tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac accaactgcc
      481 agactgatca gtgtcagaaa taggctgaac aatccagcat attccatcca ctgccatctc
      541 gaatttttca tattagaagc tccattcatc cagtgattgt ttcacatagg tagctgggat
      601 aggcaaccca ggtacatctt cttcttttgt aaaacctgat aaaacgagca aagtatctat
      661 attattgcgt atcccagctt ggatatctgt ttcatagtta tctccaacca tgattacttc
      721 ttctttttgc agccctaata cttcaacggc tttatccata atgatggctt ctggttttcc
      781 gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa gagatgactg accctgctcc
      841 aggaagaagg ccgcgttcag taggtatatt cttatctggg ttcgttccaa tgaaagtagc
      901 acctttttga atagcaagtg ttgctttaac tactttttca taggtcagat aattatctaa
      961 gcctaccact acatagtcag gagcctcttc ttcccatgta aatccggcag ctaaaatcaa
     1021 gtcaatcaat cctgcttctc cgataacata gacttttttc ccttttccat ctgctttcat
     1081 aaaatcgatt gttgccaaag tagctgtgta gactgtctct ggagctacat gaatgtcaaa
     1141 ctcatctgct agacgattag ctactgtttc tggtgactta gtcgtgttat tggtaacaaa
     1201 caagaaaggt aattttcttt tttgcaattc ttccacaaat cgtttaccgg ccggtatggg
     1261 ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa tatcctttat agtccattca
     1321 tccactactc ttcccttttt ctgatcgtaa atgctgtttt tgttttttct acttgtttag
     1381 gttgttcttg tttttttact ttttcagagg tttttgctga ctctgttttt cgatttttaa
     1441 taaccggttt tttgcgatta gatgcgactg gtcctttttt ctcatcgata tacgcacgat
     1501 tatggttttt tttctttttt cggcgctgag ttgtcttttc ttttttaccg ccgattctct
     1561 cgatgacaaa atacgcacaa ccaaaattgc aatattcgta aagatagtct tccaaggaat
     1621 cgattcgctg atctggatgc gcccgtcgat tatccgcttg gaaaaatcct tttaggcgta
     1681 gttgttcata accccaatct cctacgatat aatcatatcg ggctagtaca tcactgtaac
     1741 gttctccaag cttttctgca ttaaatcctt cacgataatc ggacacaagc cgataatgtc
     1801 tttcacctat caaaaaatta ctgtcatcta acagtgtcac tagctcgcct ttgacttttt
     1861 ctgctttagg ctcctcaacg atttcttcca aagcagctgt cagttcttcg gtgactgttg
     1921 tttcctgttc agctgatgat tttttcttag caatttctgt tttatgctca acgttctgct
     1981 gttcattttg ttttgcagac atgcgttcac cacctatctt ttttattata ccttgttttt
     2041 atttgattgg gtagtgagct ttcaagtaat ctcctaccac acttcggata aattcaggga
     2101 acaaaaattc attttttcca gcaatctcaa tcgttggaaa aaatggaaca aacatcaaat
     2161 gatctacggt agtaaaagta taatttttat tcagatcgat tggcttgttc ttccaaaaga
     2221 cttgatggtt aatagaatca tatgtgattc cattatagac catctgccca aaaatcttac
     2281 cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa gttacgattt ttttctattt
     2341 ctaaaactaa gcggatcaga tcatttccac ccaatgtgac atttaacaag tgcatcggat
     2401 gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc ttttggaatt tcagttaaaa
     2461 acagaccact gtttaatatt gctgcttccg tctttcctcg ttctttcacc gcctcaaagg
     2521 ctacttggat aaacgaatgc tcattgaaga tatctagact cagagaaaat ggcaaatctg
     2581 ctacttcttt ttcttttaat aattcatgcc cacgttctag ataactattt atttcttgtt
     2641 catcttctgt aaaagctgtc attgtttcgg tagggatggc tttggctgaa tgcttaagga
     2701 tttttttctc ttcatcgatg gttaaatgga cttcaccgac atactgcccg tactttccag
     2761 cggctgctat ttggacatca ttgattacgt gaccttctct gaataaatga tgggtgtgcg
     2821 atcctaaaat aacatcaatt gctggtattt cttttgcaat ttggaaatca tcctgaatcc
     2881 cgagatgact catcaaaact aaaacatcca cttgtgaacg taaatcttcg actaattctg
     2941 gcaaaatatc ataaggattc cgtatgtccc agccattcgg attataagta agcggaaatg
     3001 gagcagtaaa tgcaattagt ccgattttcg tctggtgttt cgttgtaatg attttatatg
     3061 gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa taagttatcc aggatgatat
     3121 caaactcagc atgatcgtat aaatgattca actcttcttt ggaatttcct acgccttcat
     3181 tgtttcctat cgtcgctgca tcataaccaa cttcattcat cagcaagata ttttcctgtc
     3241 cattcgttgc ttctgacaat ggatgccaac gatccacaaa atcacctaga tctactgcaa
     3301 tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa aaccttctga
     3361 tccttggcca attttctaaa tgagaatgca gatcattcgt atgtaaaatc actatttttt
     3421 ccatgtaccc tctcgcctct ctttatcttt atcataccat aggcagcttt ttcttccatt
     3481 gaaacaagct ctatttcaga gtttttcctg ccttgaagca aattgtataa gtacaatatt
     3541 cgataaatca gtctggctaa aaaatcagga acattcattt ttacagtact ataaaaataa
     3601 aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat cagacacatg gcggaacctg
     3661 cttagtgctg cttccttcca gacctgacac gcttcacaag cccaccattg tcctagcctt
     3721 tcggcaaaga atagtatacc ttatttttca ttcgttgacc agtctttttt taatttcttt
     3781 tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca tcccacattc gtcttctaaa
     3841 atgccacctt ctacatatgc ttggtggtta aaccgctcgt cctccaaaag attcatcaag
     3901 gtaccggctg ttccgccttt tggatcatac gcgccgaaat aaacttctgc tacacgagac
     3961 aaaatcatcg cgccgctgca cataggacaa ggctctaagg taacaaaaag tgcagcatct
     4021 tccaagcgcc agcttcccac tttttcacag gcttccttga ttgcaagcat ttctgcatgt
     4081 gtagtagcat cttgcgtggt ttctctaagg ttatatcctc ttccaatcac ttttccatcc
     4141 aatacaacca ctgctccaat cggaacttca tgaagcattt ctgctttctt tgcttctgcc
     4201 aatgcaaggc gcatccattt ttctttttct actgcatcaa gatttgtatt taccaattac
     4261 ccttccctgc ctttgttttt tcttgtttta ctttagcata tgataaaaag gctgtaacta
     4321 actttttttt ctatggtaaa ataaatacaa gaggtgaaca agatgaaaat ggataaatta
     4381 cttgctcttt acccacaagc acagaaaaaa aactctccag catcagaagc ggatatcctt
     4441 tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag attcattatc tcaacaagaa
     4501 accagccttt tgaaagcgtt attccctgta atcaacgatc aaaaaaaaca cccatggtat
     4561 cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa gctttcgttt gatccagctt
     4621 cacgtcgaat caaaaggaga attcttaaaa aaagaatggc aagatactat tttggaatta
     4681 tttcgctatt ctgctgattt ctttttttat acagacacag atgcgctctt agtcgaacaa
     4741 ataaaccgtg attatcttga tgctactgaa atcaacggta tttttctatc tttagatgct
     4801 gattttgata cgacaacgtc tgtattcgtt ggttctttcc attcacctgg caccgacctc
     4861 gtccctttgt ttgctgaaga aagaaaaatt tttttaacag agcaaaacaa tctttatact
     4921 cacagccgta ctttttctat gcaagatgtg gctttacatt actatacaaa agatactgta
     4981 aaagatagta agttgatcaa aagttacata gaactgatca aacaaaatga aatggaagtg
     5041 atcattcaag aattatggaa tgaattaggc aatgtcagct ctgctgcaaa aacactgttt
     5101 cttcatcgta atactctaaa atatcgaatc gaaaaatttc aagaacagtc tggtttcaat
     5161 ctaaaaaaag ccaacgattt actgttttgc tatcttttat tgcttcatga acataattaa
     5221 agatagatta aaggggttgt gacaataatt ttgtcacaac cccttatttc gaatgaatag
     5281 tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa aatgcattag ccggtctttt
     5341 acatcttcgg ttgaatcttt gattgaatca ccataaacag agattttctt ttttcctgat
     5401 ttgatttttg ctaacattcc catcaaagaa tccaagtcac tatctgaaag accatctacg
     5461 atttctagta atttttcatt tccatcaaat ttgtcataga cgaatttttt tactttcgtc
     5521 cgagtagtca tatgatggat cttttccatt acttttcccg atacaacgac tgctgtcgct
     5581 acactggcag cggctgctac gcttaatccg attgttactt ttgttgatgt tttcataagt
     5641 atcagctcct ttttatctat ctccatcata gcatagacaa aaaaataaac gaactaatcc
     5701 actcagtgaa atctcaatcc tttagggaaa aagttcttta ctgttccttt tacttgtttc
     5761 ccaaatccga caggtatctt atctaatact agcaaaaccc atccttgatt cccatcacgt
     5821 tgaaatgttt ctcctgctgt gtaagattgc cattcctttt gagtgatcgg caagcaaggt
     5881 atattctcta tcttttttgt agcaagcgct aatgcatagc taggttcaaa tcgattcttt
     5941 ttgaaatctc ctaaatgcaa acctgtacgt acgactttta aaccatcgag agaaggggct
     6001 aactcaggaa cttcccaaag atggtcgttg aacactaaaa gtcttccagt agcttcataa
     6061 tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct cttttgtcat ttgcacttta
     6121 cttttttttc gtgtcttctt ttctttatgc atctgatttt gtccatgaaa agtcagcttt
     6181 gcaacaaaat gtccttcacc ctgatcttta tgaggccata tacgtattgt tttttccaaa
     6241 ccagctacgc ttccccattc agaacgtcct gaactaacgg attgagtcaa aggaatctcc
     6301 tcaatcgtta ctggataatt ttctacaagc cagctaatga tctcttcatt ttcttctggt
     6361 gcaaacgtac aagtcgaata aatcagttgt cctttatttt ttagcatttt tattgctgaa
     6421 gacaaaatct cttgctgacg tttttgacag tatagcggag attcttctgt ccactctttt
     6481 attgcatttg gatcttttcg gaacattcct tctcctgaac aaggagcatc aaccacgatc
     6541 cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg gtgcatgatt tgtcactatt
     6601 gcgttcgaaa ctccccatcg ttctatattt tctgataaaa tcttcgcacg tttcggaaag
     6661 atctcattcg tcactaataa ccctttcccc ttcatttgag cagctaattg ggttgattta
     6721 ccaccgggag ccgcacacaa atccaaaact ttttcaccgg gttttgctgc agcagctgtt
     6781 ccaacgatca ttgcacttgg ttcttggcta tattcatacc cagcttgatg gaggaaagac
     6841 tttccattga cagtgccaag aaatccctca ttgctgtagg gagcgggttg taattcttcg
     6901 ctatggtaag tttggaccat gtccaatcct gccggtttta gtggattcca ccgaaatcct
     6961 tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg atgcttcttc gcctaaaaga
     7021 agacgatatt ttttgataaa ttgctgtggt aacgttgtcg cttcctcctt cattccaatg
     7081 tttcgttaga cattttacaa tcttttttac gaaaaataaa ggtggctgat ctactttttc
     7141 atgttgctgc ctaaggtgac tgttccttta agatattttc caagtattca tccgtcacct
     7201 cgtttttatc ccaaacagcg ttcatcattt caagaaaatc ttgagaagaa tcatctgagc
     7261 gcaaacttcc caatgcatta agccatacca attcaagcga cttttcttct ttgatcttcg
     7321 tgtgaccttt gacatatgag aaaaatggcc gcttaaatag cgaatccatg tctaaatcag
     7381 attttgcaat gatttcacga atactttcat ggatagaaaa aaaatctttc aataagtaga
     7441 caatgcttaa aacatcatcg gaactgcatt cagctaagta gttttcaaaa taaacagtga
     7501 tttcttctaa tatcttctct tttaacccgc tgattgattt gaaatgtgta taaatcgggt
     7561 atgtagaaca accgcagaac tctgctaact ctcttgcagt aattgacttc attccatatt
     7621 cttccacgta taccatactt ttgaacagca aatattcttt actataattt gttactctcc
     7681 ccatcattct tacctccgtc tcttttcatc tacccataaa taaataacat aaataattct
     7741 aaaaatatta ttgataatta tcaaaacata tttagttata tccattaaaa atcaattttt
     7801 ccgccttaaa aacaagaata tcttgacaaa ataccctagg aaataaacaa tagaataaac
     7861 aaggtaacaa aaaaattaaa taacgttata taaaaaagaa ggcgacactc tgtgagaata
     7921 acacagactg aaatgacttt ccactcacag tttttgtcta actatataca aaaacagaat
     7981 aaaaaggagg tatttatgct aatt
//
LOCUS       DANKAY010000118         7971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000118 DANKAY010000000
VERSION     DANKAY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            41..742
                     /locus_tag="PWY98_002514"
     CDS             41..742
                     /locus_tag="PWY98_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2038225.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1351..2367)
                     /gene="tsaD"
                     /locus_tag="PWY98_002515"
     CDS             complement(1351..2367)
                     /gene="tsaD"
                     /locus_tag="PWY98_002515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2038226.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2364..2828)
                     /gene="rimI"
                     /locus_tag="PWY98_002516"
     CDS             complement(2364..2828)
                     /gene="rimI"
                     /locus_tag="PWY98_002516"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2038227.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2835..3377)
                     /gene="rimI"
                     /locus_tag="PWY98_002517"
     CDS             complement(2835..3377)
                     /gene="rimI"
                     /locus_tag="PWY98_002517"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2038228.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3361..4083)
                     /gene="tsaB"
                     /locus_tag="PWY98_002518"
     CDS             complement(3361..4083)
                     /gene="tsaB"
                     /locus_tag="PWY98_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2038229.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4274..5254)
                     /locus_tag="PWY98_002519"
     CDS             complement(4274..5254)
                     /locus_tag="PWY98_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2038230.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5278..6762)
                     /gene="galT"
                     /locus_tag="PWY98_002520"
     CDS             complement(5278..6762)
                     /gene="galT"
                     /locus_tag="PWY98_002520"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2038231.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6774..7763)
                     /gene="galE"
                     /locus_tag="PWY98_002521"
     CDS             complement(6774..7763)
                     /gene="galE"
                     /locus_tag="PWY98_002521"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2038232.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2242 a         1709 c         1407 g         2613 t
ORIGIN      
        1 tgacaataaa tatagtgaac aataaggaga ggacttttta atgcgtgtta ataaaatcga
       61 ttttcacttt tcaaaactca gatatcaacc tgattttgaa catttctcgg atgaagagtt
      121 ccaacagatc aaggaacata ctgttcttcg ttcttataaa aaaggacaga tccttttcga
      181 cgatggtgat cccagacaac ggttgtatat cttgaccaaa ggactcgttc gtttagaacg
      241 ttatgatgaa agtgcaactt attactacta tgactatgtg aatacaaata acctttttcc
      301 tatgtcagga atgtttggag aagagaccta cacttataca gctcaagcga tgactgatat
      361 tgaaacattc taccttcctg tttcctacta tgaaaaatta gcacgaaaaa atcccgaaca
      421 gttgatctat atgatacaaa aaatttctaa agtagttgaa atacatgaat tgcgtatcca
      481 aatcggattg acctctagcg cctttgaccg ggtaaaacaa aatttattca ttttgaaaga
      541 agaattgggc gtaccaacaa aagatggtca tatcaccatt ccttatccga tcaccttaaa
      601 agaattagca gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa tcaaacaact
      661 acgtgattcc gggctgttag attatgacaa aaagcgtttt actttctgta agacagctac
      721 tactccagct atgagagaat aacgcgaaca aaaataagca gtgaccctga taaatatcag
      781 ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt agatcactgg
      841 acaagactca aagattttct atgtgtgttt cagttatgta atattcactg tttgcaaaaa
      901 atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat tattcgatat
      961 tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa tgataaataa
     1021 gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat agggctttga
     1081 caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac tcttcggtca
     1141 caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct tgtgaatcag
     1201 agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt gcatacgcgg
     1261 taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg ttcttattac
     1321 ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc ttggatcagc
     1381 attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg ctgctccaat
     1441 cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta cctcagggag
     1501 tttagttgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg ctacacctcc
     1561 agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt tagtaatcaa
     1621 tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta gatcttctcc
     1681 acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc taaaactgaa
     1741 atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt ttccctgatg
     1801 agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc ttccaacttt
     1861 gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat atgatccatc
     1921 ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca tcaatggaaa
     1981 ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt ttacaggaat
     2041 aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa tcagcaacga
     2101 gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg ccgacacacc
     2161 agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat ggcggctagc
     2221 tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag caacgatgtt
     2281 agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac aacttgattc
     2341 aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt tctgctcata
     2401 atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa tatacgaaac
     2461 ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc taaaaaaaca
     2521 tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg tgcatgccct
     2581 tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc tattactttc
     2641 gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata atcagagtga
     2701 ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg tggataagca
     2761 ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc tttttttctg
     2821 caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc atcttctttt
     2881 gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa accccaattt ccgataaagt
     2941 ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca gcgattcatt
     3001 atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg ctgatatgcc
     3061 ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga accgataaaa
     3121 ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg tgacttcaat
     3181 tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc tcgttcgaca
     3241 tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg acgatcgatt
     3301 tgttcgactg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct ggcatacgct
     3361 ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc atttttcttc
     3421 tgcttctact cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa ctggttcaac
     3481 taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg gaactgtatt
     3541 aatcatcgca ctaggcaata tttcctttat ttcagagaag aatttgtgac aatcactccc
     3601 gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag aagcagaaat
     3661 atgtacatct tcaagtacag tttctaatct attttctttc cagcggtatg ccccagcgta
     3721 tacatttttt cttcgagcat caaacatggg cacaataata ttatccacac cgatgcaatt
     3781 agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg tataagcaat
     3841 cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac catcagaaac
     3901 cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt ggtcgattgc
     3961 aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc caagaatttg
     4021 ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc ctaatgtaat
     4081 catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat acttttttat
     4141 tctgaggatg aaaataccaa aggcaaaact agtacaataa tacatgatta tctgctggcg
     4201 ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcactcca ttttcccaca
     4261 gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc acaataatct
     4321 ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact gctagctctc
     4381 ccatccattc ggtataaact tttacagtag aaagcgaggg cgttacatat ttcgctacac
     4441 tgacatcatt gaagcccatc acagaaatat cttctggtac cactaaacct ttttcttgaa
     4501 tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca tcaggaacat
     4561 gcggattcgt ttttaaatac tgcttcactg cttgatatcc tgactcgaca gaaaaatctg
     4621 cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga aatgccataa
     4681 ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca gcaagcatag
     4741 cgatatgttg cgctttatgg gagacaaagt gttcaatgac tttttgaaca cttgacttaa
     4801 agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc aaaagcaagg
     4861 cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct agtgcgatcg
     4921 ttccatctgc cggaagatca ctaatggaat cccacgtttc tttcaataaa gtaacgtttg
     4981 cttcttctgc ctttttttcg attcctaggc gaatagataa ataataaaga tcttcaagct
     5041 cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt gctgcatttt
     5101 tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc tttgtggcta
     5161 agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca ggagaaactc
     5221 ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt tttacgttta
     5281 taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct tgaatactcc
     5341 agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga catcggtgac
     5401 cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg gacgatgata
     5461 ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta gttctggttt
     5521 caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt tctccttttt
     5581 gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt ctgaaacatt
     5641 gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg caattggcgt
     5701 aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg tttcatccga
     5761 atatgctttc catttttcca atactgagtt agctgcttca ataagcaagt ttttctctgt
     5821 tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga cttgcggata
     5881 atcttctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt catgacgtcc
     5941 tgcctgataa tgatcatgag aaagaatcga tcctccaaca attggtaagt cagcattcga
     6001 accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa atgtttcctt
     6061 tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat tgtaataagc
     6121 ataaggggaa tattggaatc cccagctttc accgtctaga ttcattcgaa taatccggtg
     6181 attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca tgcaaagcat
     6241 gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt ttggatcttt
     6301 ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact ctgccggaaa
     6361 tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac ttaaattaaa
     6421 gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga atgcattgac
     6481 gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct cttgttctgc
     6541 tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat ccaataagtc
     6601 tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt ctcctaccat
     6661 ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc cagcttcgat
     6721 cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt cccctattct
     6781 tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat cgctttgata
     6841 tctgtgtatt caggtttcca gcccagaatc tctctcgctt tttcacttga agcgactaat
     6901 cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt tcctgtcact
     6961 tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc taaattgaag
     7021 aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc agcaatcaaa
     7081 tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata atcgtctcca
     7141 taaacagcca aggcttttct ttgaccaaga gctacttgta aaatgattgg gacaagatgc
     7201 gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc tacattgaag
     7261 tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat cattttttcc
     7321 atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc ttctgtgatt
     7381 ggagactctt tcggctctcc ataagttgct gcagtagaag agaatacgat tttattcacg
     7441 ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt gttgaaatac
     7501 atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg gattactcct
     7561 tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat gtctccttca
     7621 taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt atctaccaca
     7681 gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat ataaccggct
     7741 ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat tgtgtttgtt
     7801 gattctatat aatttaaaaa acgaagttta cttttatggt gaaaacaaac aaagtaaaat
     7861 ttcgtaaatt catatggtaa atttataata aaacatttag taaacatttt caaggatttt
     7921 ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt tctcttttgt t
//
LOCUS       DANKAY010000119         7959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000119 DANKAY010000000
VERSION     DANKAY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(133..306)
                     /locus_tag="PWY98_002522"
     CDS             complement(133..306)
                     /locus_tag="PWY98_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2038233.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="PWY98_002523"
                     /pseudo
     CDS             754..3116
                     /locus_tag="PWY98_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="PWY98_002524"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="PWY98_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2038234.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="PWY98_002525"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="PWY98_002525"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2038235.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="PWY98_002526"
     CDS             5704..6489
                     /locus_tag="PWY98_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2038236.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="PWY98_002527"
     CDS             6502..7353
                     /locus_tag="PWY98_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2038237.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="PWY98_002528"
     CDS             complement(7477..7929)
                     /locus_tag="PWY98_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038238.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2671 a         1360 c         1695 g         2233 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctact
//
LOCUS       DANKAY010000120         7957 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000120 DANKAY010000000
VERSION     DANKAY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            142..558
                     /locus_tag="PWY98_002529"
     CDS             142..558
                     /locus_tag="PWY98_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038239.1"
                     /translation="MQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPE
                     IHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYD
                     LIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(687..1961)
                     /locus_tag="PWY98_002530"
     CDS             complement(687..1961)
                     /locus_tag="PWY98_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2038240.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(2140..2787)
                     /locus_tag="PWY98_002531"
     CDS             complement(2140..2787)
                     /locus_tag="PWY98_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2038241.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2795..3481)
                     /locus_tag="PWY98_002532"
     CDS             complement(2795..3481)
                     /locus_tag="PWY98_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2038242.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(3487..4560)
                     /locus_tag="PWY98_002533"
     CDS             complement(3487..4560)
                     /locus_tag="PWY98_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038243.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4782..5456
                     /locus_tag="PWY98_002534"
     CDS             4782..5456
                     /locus_tag="PWY98_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2038244.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            5461..6801
                     /locus_tag="PWY98_002535"
     CDS             5461..6801
                     /locus_tag="PWY98_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2038245.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6950..7936)
                     /locus_tag="PWY98_002536"
     CDS             complement(6950..7936)
                     /locus_tag="PWY98_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2038246.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2389 a         1528 c         1436 g         2604 t
ORIGIN      
        1 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
       61 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
      121 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
      181 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
      241 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
      301 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
      361 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
      421 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
      481 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
      541 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
      601 ggctcttcgt caaattgtgt tggcgaagac aaatttgaat aaaacttagt ccgatatgcg
      661 gcgcgttctg tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag
      721 atccgtgccc gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg
      781 atcttgttat tcagcccctc agtgacgccg tttgagtggg gcaccttaaa ggcatttgaa
      841 atacccgatt ggtagcgatg gaagatcgtg aatttctttt taaacgactg aggtaacgta
      901 gatgaaattg ttttaagggt ttcttgaaag ccttccgtat ctcgtttaac gaaataatag
      961 cgtaataatt gacaaaaatg atacgcagtt cgcaattctt cgttgtagct taacagctca
     1021 tcaacgattt ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagt
     1081 ggctccaatg tcgtggaatc tttgagtaag agtttccaat atcgcttgag ccgacggtac
     1141 ttcttctgat tttcagagtg tgaggttcga aaacgattca tgatctggac acgtaattga
     1201 ttgaagctgc gattgatgtg ttggacaata tgaaagcggt cagttacgat ctccgcattt
     1261 ggaaagactg ttttaagcaa ctgacaatag ctggcattca tgtccatcac taggtacttg
     1321 acctttaaac gggcctttct aggaaagcgc aggaagtatt gagttagttt gtaaagtctg
     1381 cgatcttcaa ggatcgtaaa tagctgattg gttgtcccat caacacaaat aaaactcatg
     1441 gaaccagaaa cagacttcat tgatttgaac tcatccatac acaacaccgt cggaaggaag
     1501 ttcatacgtg gctggtaatg cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc
     1561 gagacgaagt acttcttagc gattagttta cgtgattgat tttcgtacag ttccatgaga
     1621 atcgcctgct tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca
     1681 gaaaaggtcc gtccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtc
     1741 aacttttctt tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt gcggataata
     1801 cgaatcgatc cacagtgttc acaatgggaa ggtgagtcat ccaatctccc tttaataaac
     1861 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
     1921 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
     1981 ggtgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
     2041 gaaaaaagtg ttagtagaaa atcgctttcg attttccacc aacacaaaat attatagaac
     2101 cataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat
     2161 ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat
     2221 gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc
     2281 ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat
     2341 tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa
     2401 caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata
     2461 tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg
     2521 atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa
     2581 atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac
     2641 ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt
     2701 tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat
     2761 tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg
     2821 tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat
     2881 gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga
     2941 cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg
     3001 gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt
     3061 taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg
     3121 ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta
     3181 aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg
     3241 ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag
     3301 tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac
     3361 caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga
     3421 cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat atttttttca
     3481 tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa
     3541 caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca
     3601 aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag
     3661 agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat
     3721 gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt
     3781 catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat
     3841 cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat
     3901 agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat
     3961 tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa
     4021 agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg
     4081 aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat
     4141 tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc
     4201 atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac
     4261 attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc
     4321 tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc
     4381 atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc
     4441 tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat
     4501 accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat
     4561 ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa
     4621 ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac
     4681 atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt
     4741 tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag
     4801 tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga
     4861 taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc
     4921 ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac
     4981 ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata
     5041 agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag
     5101 aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg
     5161 ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac
     5221 aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg
     5281 gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga
     5341 cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat
     5401 tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca
     5461 atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca
     5521 tttgctttta tcttttttct tttgggattc attactttac agatattaaa ccaatctgca
     5581 tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta
     5641 gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac
     5701 agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg
     5761 atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac
     5821 caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta
     5881 aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg
     5941 aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca
     6001 ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt
     6061 ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga
     6121 caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa
     6181 aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc
     6241 atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca
     6301 atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa
     6361 tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt
     6421 attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt
     6481 ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg
     6541 gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc
     6601 agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg
     6661 aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat
     6721 catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg
     6781 ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc
     6841 acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc
     6901 gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt
     6961 tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa
     7021 catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac
     7081 gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt
     7141 catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga
     7201 tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt
     7261 tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt
     7321 ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg
     7381 tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca
     7441 atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag
     7501 tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca
     7561 ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat
     7621 cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat
     7681 cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac
     7741 catccatccg attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc
     7801 tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac
     7861 gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta
     7921 aatttttctc ttccatgtaa attcctccta gcaaata
//
LOCUS       DANKAY010000121         7889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000121 DANKAY010000000
VERSION     DANKAY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..341
                     /locus_tag="PWY98_002537"
     CDS             <1..341
                     /locus_tag="PWY98_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HDL2038247.1"
                     /translation="SDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWI
                     DFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQ
                     DIIHRVDDWI"
     gene            complement(1034..1972)
                     /locus_tag="PWY98_002538"
                     /pseudo
     CDS             complement(1034..1972)
                     /locus_tag="PWY98_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(1972..2589)
                     /locus_tag="PWY98_002539"
     CDS             complement(1972..2589)
                     /locus_tag="PWY98_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2038248.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2603..2773)
                     /locus_tag="PWY98_002540"
     CDS             complement(2603..2773)
                     /locus_tag="PWY98_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038249.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3121..4611)
                     /locus_tag="PWY98_002541"
     CDS             complement(3121..4611)
                     /locus_tag="PWY98_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2038250.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(4654..4953)
                     /locus_tag="PWY98_002542"
     CDS             complement(4654..4953)
                     /locus_tag="PWY98_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038251.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5319..5603)
                     /locus_tag="PWY98_002543"
     CDS             complement(5319..5603)
                     /locus_tag="PWY98_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038252.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6122..6607)
                     /gene="vanZ"
                     /locus_tag="PWY98_002544"
     CDS             complement(6122..6607)
                     /gene="vanZ"
                     /locus_tag="PWY98_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL2038253.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(6760..7671)
                     /locus_tag="PWY98_002545"
     CDS             complement(6760..7671)
                     /locus_tag="PWY98_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL2038254.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2364 a         1442 c         1176 g         2907 t
ORIGIN      
        1 aatctgataa aaacttcatt tctttttata ataattataa aaatgacttg cttgaatata
       61 aagataaaga agaaatgttt cctaaaaaac cgatacctga aaacaccata ccgatttcaa
      121 tgattccttg gattgatttt agttcattta atttaaatat tggtaacaat agcagctttt
      181 tattgcctat tattacgata ggtaaatttt atagtgagaa taataaaatt tatataccag
      241 ttgctctgca acttcatcat tctgtatgtg atggttacca tgcttcacta tttatgaatg
      301 aatttcaaga tataattcat agggtagatg attggattta gtttttagat tttgaaagtg
      361 aatttaattt tatacacgta agtgatcata aaatttatga acgtataaca accacatttt
      421 ttggttgctt gtggttttga ttttgaattt ggttttgaac ttatggactg atttattcag
      481 tccatttttt gtgcttgcac aaaaactagc ctcgcagagc acacgcatta atgacttatg
      541 aaacgtagta aataagtcta gtgtgttata ctttacttgg aagatgcacc gaataaaaaa
      601 tattgaagaa caactagcaa aagattttaa agagttattt tattttaagt ctttataaca
      661 tgagtgaagc gaatttttaa atttcgatag aaatttttac atcaaaaagc cccctgtcaa
      721 aattgacgaa gggggttttt tggcgcacgc ttttcgttag aaatatacaa gattgaaaat
      781 cgtgtataag tgcgcccttt gttttgaact tagcacgtta catcaatttt ttaaaatgat
      841 gtataagtgc gcccttttaa attttgagtg attatatttt ttgagttaga aaaagggatt
      901 gggaaaattt cccaaaataa tttaaaaaat aagcaaaaat tttcgataga gaatgtgcta
      961 ttttttgtca aaggtgtata ccttgactgt gcttgctgtt acattaagtt tatttttaag
     1021 ttattaaaaa agaaatagct tttaaagttt ggctcgctgt cgctttataa agctgattga
     1081 cttttgattg caaactactt aaagaaaaca aactcggact attcgttttc ttctctttgg
     1141 tttgaacatc agcaattatc ccctcttgat tgcctatttt agcttgttta gaagaaacaa
     1201 aagctaaaag ctcctcttgg gttttaaaac gctgtgtggg gcttagaacg cccttaaacg
     1261 acccttggtt tacttttata ctagcttcca cctcgaaaaa aggttctttt ttaaaattct
     1321 ctatggcttc ctggcgctga aaaataaggt ataaggtggg cgtttgaaca cgtcctagtg
     1381 aaaatgtacc ttgtacgccc ttctgttgta aatttaacgt atacaaaggg cttgcgttca
     1441 tgccgatcaa ccaatcggca atttggcgtg tttgcgcttc ttgataaaag ggatagtaat
     1501 tcattccagg ttgcaaattt tgaaaaccgc ttcggattac atctttttct aagctattga
     1561 tccatagtct tttaaatgtt ttatcttttg aaaaggcatt tgctttatgg ataatcgacc
     1621 aggcgatatt ttcaccttct ctgtcgctat ctgttgcaac aataattgta tttgcctttt
     1681 tgagaagttc tgcaacaatt ttaaactgct ttcccttatc ttttgcaact tcaaaatcgt
     1741 atcgatcagg aaaaatcggc aaagattcaa gtttccaatt ttgccacttt tcgtcataat
     1801 gacctggttc tgctaattcc actaaatgcc caaaaccaaa ggtgataaac gtttcatctg
     1861 taaatagtgg gtctttgatc tcaaaataac cgtctttttt ggtgctttgt tttaaagcac
     1921 ttgcgtaggc taatgcctgg cttggttttt cagctaaaat aaccgtactc attaactatc
     1981 cctcttttca ttgttttttc tttgatcgac tgtcacgtta tatcttgctc gataccttct
     2041 aaacgttcgg cgattgattc cagtttgttc ttcaacttct ttatcggata aaccattcaa
     2101 aaacaaatcg aaagcatgct ttaaacgtgg atcattttct ttaaatttat gctgacgacc
     2161 tttgaatttg ccttttttct tagcaatttc gattccttgt gcctgacgtt ccttaatttt
     2221 ttttcgttct gattctgctt gatacttgta caattcaatg acaaggctat taatcaaacg
     2281 ccttaaattt tcatcttcaa taccattcat tgagggtaaa tttaagactt ccagggttgc
     2341 ccccttaatt tgaatttgat tcatcaattc tgttaattct ttattatttc gtcctaatcg
     2401 atctaattca gtaacaataa caatatcccc ttcacgaata tagttaagca tagcttgtaa
     2461 ttgtgggcgt tcgaccgatt gaccgcttaa tttgtctgaa aagaccttag aaacgccctg
     2521 taacgcttgt aattgccgat ctaagttctg ttctttgcta ctgacacgtg cataaccaat
     2581 tttagccatt ttcaaccaac ctctaaaatt ctctcggttg caataaccaa tcagcaatat
     2641 ctactttttc aatttcaaat tgcttatcag aaattgtctt ttcgtaagcg ataaaatctt
     2701 gcgcatattg ttgctcatta aaaatagcca ccacttcgtc attttctaaa actcgataaa
     2761 taaatttttt cattttactc ctcctattat gcccaactta aatgacctat tcaccaagtc
     2821 aattatactg ctaaaatcat attaggacaa ataggtatac tctaatgacc tataaaagat
     2881 agaaaattaa aaaatcaagt gttcgcttcg ctctcactgc ccctcgacgt tttagtagcc
     2941 tttccctcac ttcgttcagt ccaagccaac taaaagtttt cgggctactc tctccttctc
     3001 cccctaataa ttaattaaaa tcttactctg tatatttctg ctaatcattc gctaaacagc
     3061 aaagaaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
     3121 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gttttagggc gttctgctag
     3181 cttgtttaaa gtctcttgaa tgaatgtatg ctctaagtca aaagaatttg tcagcgcctt
     3241 tatatagctt tctttttctt ctttttttac tttaatgatc gatagcaaca atgatttaac
     3301 actagcaagt tgaatgccac catttcttcc tggtttaatc ttaaagaaaa tttcctgatt
     3361 cgccttcagt accttcagca atttatctaa tgtccgttca ggaatgccta gcacttctct
     3421 aatctctttt ttggtcgtca ctaaataagg cttgtataca tcgctttttt cgctaatata
     3481 agccattaaa tcttctttcc attctgacaa atgaacacgt tgacgttcgc ttcttttttt
     3541 cttgaattta aaccaccctt gacggacaaa taaatcttta ctggttaaat cacttgatac
     3601 ccaagctttg caaagaatgg taatgtattc cctattagcc ccttgatagt tttctgaata
     3661 ggcacttcta acaattttga ttacttcttt ttcttctaag ggttgatcta atcgattatt
     3721 aaactcaaac atattatatt cgcacgtttc gattgaatag cctgaactaa agtaggctaa
     3781 agagagggta aacatgacgt tattacgccc tattaaaccc ttttctcctg aaaatttcgt
     3841 ttcgtgcaat aagagattaa accagggttc atctacttgt tttttgcctt ctgtaccgct
     3901 taaaaccgtt agacttgaac gagtaaagcc cttattatct gtttgtttga aagaccaatc
     3961 ttgccattct ttgaaagaat aacggtaatt aggatcaaaa aattctacat tgtccgttct
     4021 tggtatgcga gcaataccaa aatgattaca cgttagatca actggcaaag actttccaaa
     4081 atattctcgg atattttgcg aaattatttt ggctgctttg acagatttaa attctgattt
     4141 tgaagtcaca tagactggcg tttctaaaac aaaatatgct tgataacctt tatcagattt
     4201 gataatcata gtaggcataa aacctaaatc aatagcggtt gttaaaatat cgcttgctga
     4261 aatagtttct tttgccgtgt gaatatcaaa atcaataaag aaggtattga tttgtcttaa
     4321 attgttttca gaatgtcctt tcgtgtatga acggttttcg tctgcatacg ttccataacg
     4381 ataaacgttg ggtgtccaat gtgtaaatgt atcttgattt tcttgaatcg cttcctcgga
     4441 agtcagaaca acaccacgac cgccaatcat gcttgatttt gagcgatacg caaaaatagc
     4501 ccctttgctt ttacctggct tggtagtgat tgagcgaatt ttactatttt taaatttgta
     4561 ctttaacaag ccgtcatgaa gcacagtttc tacaacaaaa gggatattca ttcagctgtt
     4621 ctcctttcct ataaatccta taaaataggt tgtttaatta acttggtttg ctttttcatt
     4681 caactgtttc aatattgcat gttttgaaaa agattttttt cctttataag tcaatttttt
     4741 tccactaatc gaataaatta ttttgttatt ttctattaac ttatatatat aatcttcccc
     4801 ctccgaagaa aaatacttat ctgattttgt ttctaagtag atatttctct tttctaactc
     4861 tttcttaaac gtttctagtg tatagatatt tgctaatttt cttatctcca ataaactatt
     4921 ttttatataa gttttacatt catcatgatt catacaaact ccaccttcta taaatgaata
     4981 caaaaaaagc aatcaaacga tttccgattg attgcttaac aattcttaaa ttcagtagct
     5041 tagatacttg aaaactctct gatttcccta tataatgata gtacggttat ataccgtctt
     5101 caaacaaagt taattaaata acttcttacg agggaagagt tcatctgact aactgataag
     5161 cgttggtttg gcaatcttat cgggctatgc atttataaaa tgtcgtcaaa cattttataa
     5221 atgtgtcatg gctctttttt cgtttctatt cagttcgttg tttcgttata tctagtatac
     5281 cgcttttaaa aaaaataagc aacgatttcg tgcattattc acacgaagtc attgcttttt
     5341 tcttcttcca tttctaaatc caatgttact tgttctgatt ctgtttctgg ttctggttct
     5401 gttggctcat ttgggattaa atccactact agcgttgagt tagttaactt tgcaatttgt
     5461 tctagtgttt ttatggttgg atctgatttt cctgattcta ttcgtgaata atttgatcta
     5521 ctcatttcta attcttggga gaatttttct tttgatttgg tacctctcat cttttttaag
     5581 ctttctctaa atgctagttc caagtttctc ccccctcttt ttgtgtaata aaattcacgc
     5641 tttatttaaa ttatagcttt taatatctta ataaacaagt gattaatcac ttgtgatttg
     5701 tgattattcc aaaagtaggt aatttcacca acctttttca aaagttttta tcgacgatta
     5761 ttggactgga tcgtgtttcc taaagcgatt ctaagcgcat gtttaatttt aaagtgggta
     5821 attctcttta aaacgtccta caaagattca aaaaaagcat ggctgctagt gggggtaccg
     5881 tcaggaaaat gcggatttac aacgttaagg aaatttatta aagagaccaa ttcctcagca
     5941 ctaaaattga taattgggct ttctttgtta ctatagtgtt aggtaagctt tttactttat
     6001 caccggtttt cccctcactt cacacctacg ggcgagtttc cccacatccc gttttccatc
     6061 taatccaatt cattcaatat ctttatgtgt atgaaagatc agattgcttg atttagacat
     6121 cttacacgta atttattctt aaatgggtac ggtaaacgag caataatacg agcaaaagta
     6181 tacctacaaa aataataact ctgtctaatt ttttttgatt catatgctta ttgcttaaac
     6241 catataattt cagtccaaga aagcctccaa cagtatttgt aattacatct gttatgtctg
     6301 tcgctccaat agcgaagata aattgaatta tttcaaaagt aagacttaaa accagtacaa
     6361 aagcaaactt aggtaaaaat ccgatttctt taaaattgac attcaaaagc aagccaaatg
     6421 gaataaagat tataacatta tctatcatct ctctgaaatt cccagtagca gtaaatggag
     6481 tcaagttaag acttctttga tgataattaa atactgataa aatattgtat tgtaatttga
     6541 ataacactaa ccagattagt gtcactaaat ataaagctag caatcctcta gataatattt
     6601 ttcccaatat aatttcctct aattcttttg tttaagttgt atcattagta tagcaatgtc
     6661 ctagttaacg tgggttttaa attaaaaagc taacgttacc ccaaaagtgc tagtacaata
     6721 atgctgttca ttaatttcgt tggtttcatt ccgccatcct tacctccttg aattagtatg
     6781 tgttgatccg ggaaacactg tcacaattac accgtctata ttgtttcctg atatctcata
     6841 acgaagatta gtcggcacat gaatggtggt atttttagta acaggataat aaaagatctc
     6901 atatttttcc ccatttacac taacagaaat ggttttttct tcttttaggt aatccatata
     6961 ttcctcgaga acgaaattct tttctttcat aatcgcacta tgtggtaaac caacatagcg
     7021 aatatgccat ggttcatatt gaattcctgt taactctgtt ttgtcctctg gataacgtaa
     7081 aatgaacccg tatttccaag cattttcttc tatccacttt ccttcagggg ctcgttccat
     7141 tttcgtcaag cttgatccta catctagtga taaacctgaa ttatgctcac tataacctgc
     7201 tggtaaggca tactcagccc ccatttcttg gtaaagcaca ctttgctcat caaagtctcg
     7261 atagccacta ttaataataa aatgactaac gccacccttt acagcatcat tgaccatctc
     7321 tgaaaatttt tgtgctattt cttttgacat ataaatatta ctatcaagca acccgtatcc
     7381 atttattaat tcgtcatgtt tagataaatt cacgatatct gacttcacac tttcttggcg
     7441 aacaggatat ttactattga ttaatagcag atttccttga taaacctgtt cttctgtaat
     7501 tgttttctct tgggtatttt cagaagtccc actattttct aaatgttctt tgggattttg
     7561 atcataattt tgaaattcga ctttttcctg agaaaacagt gcttcattaa cgtagtcata
     7621 acctaagtat attaagaata acaataacaa taaaaaaaac aacttcttca ttttcagtct
     7681 cctccttaac tatatagttg gaagtatgaa atatttctag aatcttcccc ttaaacggca
     7741 caatctcgtt tgagattgtg ccgttttgcc ccgtaaatta cagtcaatta tttcgcccta
     7801 ctggatttac tattatcacc aatgtagatg aattttcata agtgataccc atgctgaatt
     7861 taacgaggct gaactgcctg ttgacaatg
//
LOCUS       DANKAY010000122         7786 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000122 DANKAY010000000
VERSION     DANKAY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            140..1120
                     /locus_tag="PWY98_002546"
     CDS             140..1120
                     /locus_tag="PWY98_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2038255.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1242..2660
                     /locus_tag="PWY98_002547"
     CDS             1242..2660
                     /locus_tag="PWY98_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL2038256.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2731..4200
                     /locus_tag="PWY98_002548"
     CDS             2731..4200
                     /locus_tag="PWY98_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2038257.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4210..4677
                     /locus_tag="PWY98_002549"
     CDS             4210..4677
                     /locus_tag="PWY98_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2038258.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4713..5585
                     /locus_tag="PWY98_002550"
     CDS             4713..5585
                     /locus_tag="PWY98_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2038259.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5667..>7786
                     /locus_tag="PWY98_002551"
     CDS             5667..>7786
                     /locus_tag="PWY98_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2038260.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGN"
BASE COUNT         2697 a         1063 c         1567 g         2459 t
ORIGIN      
        1 aacagctatt gccagataaa aataaatgat attatatata taaatactta tccgaaaagg
       61 ggaatgagag agtcaaggtt cttggaatgt cgaatagtcg agtaggtaga ttttctatct
      121 acaaccttgt tgtttaaaaa tgaaaaaaat tggaattaga gaagttgcga aagaggcaaa
      181 tgtttctcct acgactgtgt cgagggtatt gaatgatcgt ggatatttga gtgaggaaac
      241 aaaaagaaaa gtttttgatg ccatgaaaaa attagaatac tatcctaatg aactggcgag
      301 agcacttttt aaaaacgaaa catataccgt aggtttgatt tttcctacaa ttactaatcc
      361 atttcacgct gagttaattc aagatatcga gttcgagctt tccagcaggg gatataaagt
      421 gttgctgtgc aatagcttaa acaatccaac aaaagaaaaa gagtacttga cgatgttgag
      481 aaagaatcag gttgatggaa ttatagtagg aacgcacaat aaagatattg cggattatga
      541 tattccaggg ctacctattg tagcgattga tagaagtctt ggtgaaaata cagttacagt
      601 atcttgtgat aattacaggg gtggggaatt agctactcaa ctacttgtaa atcatggctg
      661 tcagaatata ctgtgtattc ggggagatag taagataaag ctacccgcga atagtaggac
      721 tttagcatat aaagatgttt tgaataaatt catgctggaa ccgttagtcc tagaagtacc
      781 atttgtaaaa gaaatcaatg aaaaaaatga aataattaag acatatctgg aaactcatcc
      841 aaatattgac ggggtttttg caggagatga tttattggct tctttggcga ttaacaactt
      901 aggaagttcg ggcaaaagag ttccaaaaga ggttaaagtg attggatttg atggagctca
      961 acaaacattg atttataatc cagaattatc aactattcaa cagcctatag atcaaatatc
     1021 taaagtagca gtaaaaaaat tattgaatat gattaagggc gaaaaggaga cggatgaact
     1081 ttcacttcca gtaaagctca taaaaaaaaa tacagcataa gttaaatcgg cgaaaaattc
     1141 cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca tatgaaacgg gttgacatat
     1201 attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa ttattaatta
     1261 aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga gatgttatgc
     1321 cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga aatccagttc
     1381 cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt cattttgaag
     1441 actttgggga atctctttta agaggggatg acaatgagag agaccagttt atctacgctg
     1501 gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat aatcgtgaat
     1561 tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat gattttgttc
     1621 attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat gatattcgaa
     1681 actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg ctaattttag
     1741 gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa tttacatcaa
     1801 aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg tataccatgt
     1861 ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat tcagaatact
     1921 cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct tggattaagc
     1981 ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct tatgatggaa
     2041 aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat aaaaacaatt
     2101 atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat ttttcacttg
     2161 gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat attgccgatg
     2221 tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat agtggaactt
     2281 tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt tcactgagat
     2341 tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta gatgaggggc
     2401 gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat aaaggactta
     2461 ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt gttgatgaca
     2521 atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat ggttgttttg
     2581 gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat accaagtttt
     2641 caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa tcaaacatga
     2701 cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc cgaagatgta
     2761 ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat tactcgttta
     2821 cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga aatagaaggt
     2881 gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac aggtataatt
     2941 gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc cgtttctgaa
     3001 atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt taaagttttc
     3061 tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt tttaggaatt
     3121 cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc tgatcacaat
     3181 gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt tgattattta
     3241 ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat tttaggatta
     3301 cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt cgtcatggga
     3361 actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt tgcgtttcaa
     3421 gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga gaaaattaca
     3481 aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac aattgttctt
     3541 gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg taatgtttta
     3601 ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt tgtttttgca
     3661 ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg tattgaggca
     3721 aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt ttcaattatt
     3781 gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc taaagataga
     3841 gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc ggctatcttt
     3901 gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg tgctggtttt
     3961 ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt gactgtattg
     4021 ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct catagcattc
     4081 attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat ccctgaagct
     4141 aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca agtaaagtga
     4201 gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc acctgcaacc
     4261 ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg aatgatggga
     4321 caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga tggttttgtt
     4381 tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg aattgagata
     4441 cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga tattaaagtt
     4501 caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt tgtagttttt
     4561 gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca agacgaaatt
     4621 ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct tggctaatga
     4681 taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt gaagctggtg
     4741 gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa aaagtcagct
     4801 ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt aatcatcata
     4861 aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt cattgtgaat
     4921 ctaaaacgta tggcttcatt acatcaacgc caaaacttgc ttggcaggat tttgatttta
     4981 ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat gtaaatgcct
     5041 cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt gtttactata
     5101 ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt gaaggtttta
     5161 gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac ttttctggga
     5221 catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca atcgaaagaa
     5281 gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa atagaagctt
     5341 attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat gtcattatat
     5401 ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat aagtttattg
     5461 agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg acaccaaaat
     5521 tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt aaagaaaaat
     5581 cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc ttttttttca
     5641 attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat gaaagatgaa gtattagagt
     5701 atatcaaaaa taatttaaag tattacgatt tttctgctca agatattgca atgaaatttt
     5761 gtattaaaag aaatgtcgct tcccactatt tgaatcagtt attttcagat ggaaaattac
     5821 taaagaatga ttctgttaga ccagtaatgt tcaagtataa tcaacaaaag ccaaaagact
     5881 gcttttctaa gtttattgga gcagacatat ctttgaaatc cactatagat aaatgcaaag
     5941 ctactgtaat gtatccccct aatggcctcc cattaataat aaaaggaaac agtggcgttg
     6001 gaaaaagttt tttagcaagt ttgatctatc agtatgctct tgatagaaaa gtaattcata
     6061 atgacgcaaa atttgttgtt gtgaattgtg cagattatgc aaataatcca gagctattgt
     6121 cagcagtttt atttggatat aaaaaaggag cctttactgg ggctgaaaaa gatactgcag
     6181 ggatgctatc ttctgctaat ggaggatacc tttttttaga tgaggttcac aatctaagtg
     6241 cagaaaacca agagaaattg tttcttctta tggattctca aaagtatcgt aaacttggtg
     6301 aaagtgtgca atgggagtat gcaaatgttc gccttatctt agccacaaca gaagataaaa
     6361 atacctcttt gttagcaact tttagaagac gcatcccagc tgaaatcact ctgccagatt
     6421 acaataatag aagtacatct gaaaaaattc aattattatt tgaatttctt aaagatgaag
     6481 caattacaat tgattcaaaa atattttgtt cgattgaact ttttactgat cttttatcga
     6541 aaacatttga aggaaatgtt ggagaattga ggaatgaaat caagcttctt tgtgcagaag
     6601 gttatttagg aaataagaga aagtcttcaa tttatttagg ggacaaacta gacagcggtt
     6661 tttggataga tccagaaatt agtatagatt taaaaaaaat gtttcttaaa tcactttctg
     6721 aaattgattt gattgaactt tttcaaaatc acatttcaat tgaagaaagc atgagtcaat
     6781 taagagatag aatacttaaa gagtgtgcag gttcttctga gtataattat ttagaaagtg
     6841 acgaatttat agcattaaga aactatgttg ttaataaaat atcaccaatt attgattcga
     6901 caggtctatg tttattagat gaattcttgg cggatatcag tctatttata atgtttattg
     6961 atgtaattga agaaacatca agaaattttt tgctgtctaa gttcagaaaa tttcgtatga
     7021 aaaatgataa acaaaaatta ttggcaaatg agatttggag tagtttagaa gtcgaagaaa
     7081 gtaaacaaga acttttgttg aaatggatac tttttcttgt aaagaagttc tatgtgaaaa
     7141 taccagaaac ccattgtctg atagttatgc acggtaaaat aacagcttct gcgatcgcaa
     7201 gggaaacaaa taagttgctg aatacatatg tttatgaggc tttcgatatg ccaattgaag
     7261 gtgagactag tgacttaatt aacctaatca acaatttttg taaaagtata gatacgacga
     7321 atggactcat tctgctagtt gatatgggat cattggaaca aatgtatgaa aaaattgaat
     7381 ccaatgtaat aggtgatttg gtaattttaa acaatgtttc tacagcttta gcgattgaat
     7441 gtgggattca agtttgccaa aaaaagccaa ttagtcattt ttaccagatg gattttgatt
     7501 cgtttcaagt taaagttcag tattataaag gattatcaca aaagaaaaat gtaatagtat
     7561 cttgtctctc tggtgagggg atttctgaaa aagttaagga tattttaaaa cggtatctcg
     7621 ataatcaagt ggagattttg atttttgatt ttaacgcatt aaagaaaata gcaaaggaaa
     7681 aagatactat gatttttaag aatacaattt gtgttctttc aaccactgaa ttttacattc
     7741 aaggaatcga ttgtttaaat ttagaaaatc taattaatgg gaatca
//
LOCUS       DANKAY010000123         7537 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000123 DANKAY010000000
VERSION     DANKAY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            107..1248
                     /locus_tag="PWY98_002552"
     CDS             join(107..335,335..1248)
                     /locus_tag="PWY98_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2038261.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="PWY98_002553"
     CDS             1483..1935
                     /locus_tag="PWY98_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038262.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="PWY98_002554"
     CDS             1949..2638
                     /locus_tag="PWY98_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038263.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="PWY98_002555"
     CDS             2763..5114
                     /locus_tag="PWY98_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2038264.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="PWY98_002556"
     CDS             5194..5589
                     /locus_tag="PWY98_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038265.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="PWY98_002557"
     CDS             5914..6531
                     /locus_tag="PWY98_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2038266.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="PWY98_002558"
     CDS             6572..6871
                     /locus_tag="PWY98_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL2038267.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="PWY98_002559"
     CDS             6905..7072
                     /locus_tag="PWY98_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2038268.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="PWY98_002560"
     CDS             complement(7117..>7537)
                     /locus_tag="PWY98_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2038269.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DANKAY010000124         7249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000124 DANKAY010000000
VERSION     DANKAY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(38..1464)
                     /locus_tag="PWY98_002561"
     CDS             complement(join(38..891,891..1464))
                     /locus_tag="PWY98_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HDL2038270.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSPVQFRKTTVTVV"
     gene            1707..2162
                     /gene="tnpA"
                     /locus_tag="PWY98_002562"
     CDS             1707..2162
                     /gene="tnpA"
                     /locus_tag="PWY98_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2038271.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2427..2525
                     /locus_tag="PWY98_002563"
                     /pseudo
     CDS             2427..2525
                     /locus_tag="PWY98_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3208..4026)
                     /locus_tag="PWY98_002564"
     CDS             complement(3208..4026)
                     /locus_tag="PWY98_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2038272.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4187..4876
                     /locus_tag="PWY98_002565"
     CDS             4187..4876
                     /locus_tag="PWY98_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2038273.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4890..6392
                     /locus_tag="PWY98_002566"
     CDS             4890..6392
                     /locus_tag="PWY98_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2038274.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6405..6881
                     /locus_tag="PWY98_002567"
     CDS             6405..6881
                     /locus_tag="PWY98_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2038275.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6978..7088)
                     /locus_tag="PWY98_002568"
                     /pseudo
     CDS             complement(6978..7088)
                     /locus_tag="PWY98_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2343 a         1149 c         1308 g         2449 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gatccgattt ttggctactg
      601 ctccgacttg tcctcgataa gaattgtagc gccgtgattt acgtgtgtat gtcgttactt
      661 ttattcctag cttcttgatc aggcgctgaa ctttcttttt attgatagag aaaccgcggt
      721 ttcttagttc attggtcatc cgtaaacagc cataatcctt gtgcttctcc cgaactttaa
      781 gcatttccgc ttcaatctct tgatctggat ttttacggtc caatcgtttt tgccagtaca
      841 taaagattgc cttagggaac tttaaagttt ctagaagctc aactaattta aacggtcctc
      901 ggaggcttgc gatgattcgt gcgattgctt cattcgtcgt tgttgggctt cctgttttcg
      961 cagcttcctc aattctttta aaaaggcatt ttcaatttga agggaacgga ctcgtttctc
     1021 caactcctcg atgcgttcac gttctttaga cgttgcttta gttgtagatt ttttcttatt
     1081 tttgggttca tttttcttag acaagatagg tggacatcct ttcgtttttg agagtccatc
     1141 gatcccaccc tcacggaatt gacgcatcca actagcgatc aaattagggt tgttcatgtt
     1201 aagtgtgttg gcaacttcac gataggacag ctcacttgtt agatataact ctatcgcatc
     1261 cagcttaaat tgaacagaat attttttctt ttgtcgcttt cttactagac cctcttcacc
     1321 aaattcttga taggcattga tccagcgctt cacttgtgat ggagccttaa tggaatattt
     1381 ctttgccaga taatcacttc cgcctttacc atccaagtaa tcatgaacga tctttaattt
     1441 aagttcaaaa gtatacttag ccataaaaaa taccgacctc caaaagttag attttttggt
     1501 ctaacttttg ggggtcggta caaaacttaa aagttttaga cccttttatt ttcttaatga
     1561 cttcagtcag ttttgtgcgt aaagcgtgcg aaacaagaaa accgcgcgtt tctacgcgtg
     1621 gaagaagtat ctttagcgat aaaaactcct ttcgatatac tagtgagtgg ctaccaaccg
     1681 ctcatcagat cgaaaggagt tttttcatgt caaatgacga taaaagttta gcatatactc
     1741 gttggaattg taagtatcat attattttta caccaaaata tcaacgaaaa gtaatctatg
     1801 ggcaattacg aaaagatctt ggaaaaatac ttaggaagtt gtgcgaaatg aaagatgtgg
     1861 aaatcataga agcacatgca atgctagatc atattcacat gcttgttcga gtaccaccaa
     1921 aaatatgtat atccagtttt atggggtatc tgaaaggtcg tagtgcggta ctaatccacg
     1981 agcagcacgc aaatctcaaa tataaatacg gaaacaaatc cttttggtcg aagagatact
     2041 atgtgagtac agtcggattg aatcaaaaga caaatgaaaa atacatccgt gaacaagaag
     2101 ctgatgatcg cattcgatat attataagta aaagagagta cgtagaacca tttaaaaaat
     2161 aaagagaagt aagtgagtga agatgagcag ttggcggtct tttaaaaatc cccttttaag
     2221 gggcagcgag tgtcgacccc ttctaggggg attaaaaata cacccgtcct cacgggtgta
     2281 tgggttactg atagacttga ttttaactgg tgtcagaaac ttatgtccag aaagggtgat
     2341 gaaattttgg caatttacgg ctattcaaga attagcacaa gcaaccaaga ttacaaaaca
     2401 caaattcaaa cattagaaaa tgctggcgca gaaataatct tcctagaaaa tcactggatt
     2461 atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg tctaacttaa
     2521 tttgacaatt aagagtatat ttacttcaga tggtaaggca acaaaataaa atacaatagg
     2581 cagatgaact aataaataaa atctgtatta aatacagata aattaatata aaaatcaaaa
     2641 ggatcataaa cttttaacaa gtgatgaaat gggtggaaat tgattcttat agacagtttg
     2701 tctcataaca ttgaagtatt ttgcaatttt agcattgaaa cactttttta atcttcaacg
     2761 ttgttcttat aaacaagatg attgggaatc ttttgaatta acactgtata gcttagaaca
     2821 ccctttatag aaaacattcg cttagcaatc ttaataattc cttgtgcccg atgtcgttta
     2881 gattcagtag gttcctgctc atcgatcatg gaaaaaattt gaaggatcag atccttaata
     2941 aatttattta agagaggatg atccattgct tcatccatac tcgataaact agtgataatg
     3001 agctacacgt ttttctttca agttgatcaa tagcttccac gataaaaata tctttagata
     3061 aaatcaagaa ccgtaggaac tacagggata tttggtcaat aagagccacc gctattagta
     3121 aaaaataagc tatcaagtat gctctcatcc caagaattgc ctacttcaat cgttaaaaac
     3181 cgttaggttt agctaagtgg gggtagttca ctcaatactt ttatcaataa tagagcgcaa
     3241 aactttttct cttaattcaa ctgaatcaat attattgttt tgttcataac catgaactaa
     3301 tagatcacaa atataaagtt gtgatatttt tccagctaaa gatccaccat taagaaattc
     3361 ttcaatggct gtttgtaaaa ctaagtctgc agactttcct ataggtgaat gaatatagtt
     3421 agttatagca ataatttttg cgccattttt tttagctatt ttcaatgaat cataagtatc
     3481 tttcgttttc ccagataaag aaaaaatgat tactaaatca ttaactgtta ataaagaagc
     3541 gacttgggct tgaaaatgag ggtctagtac agcttttgct tgaactccta cgcgaagaaa
     3601 catattttct aaatcaagac tagtatttcc actagatccc actccaaata tatatatgtt
     3661 ttttgattga gtgatcattt taatggcttc attcaatttt tcttcgtcta aaagcctaat
     3721 agtggaatag agagcttctg ttaaactatt agcaatttca tcatagtact ttacactagt
     3781 cggatgttct tttttttgac taaagtcttc ctttgcaatt tcaattttta aatcagaaaa
     3841 accagaaaaa ccaatttttt gacaaaagcg aataatagta gcatcgccaa cttttgtgtt
     3901 tttcttaata tcactcatcg tactataaat aatagttttc ccagaattta aaataaaatc
     3961 agcgactttt ttttcagact tcgttaaaaa tggataattt tctttaatta aatagatata
     4021 actcatacaa actcctctac aatttcaact tttctccatt atactccaaa ataataaaaa
     4081 aaactcctaa atactccatt tttatcttta cttttatgaa agcggatgct atattgtttt
     4141 tggagttatt atccaaaaat aataaaaaat tggagcgttt aggataatgg ataaaaagag
     4201 gtttttagat cttatttcaa aaggtttgat tgtttcatgt caagcattac ctggtgaacc
     4261 attttacaaa gaggaagaaa gtttaatgcc ttattttgct ttggctgctc aaagggctgg
     4321 agcagttggt atacgtgcaa attctgttcg agatattatt gaaattagaa agtgtgtatc
     4381 attaccaatg attggaatta ttaaaagaga ttatccacca gaatctccat tcattactgc
     4441 cactatgaga gaagtagatg aattagtaga aaccggtgtt gaggtaatcg ctcttgattg
     4501 cactcttaga aatcgacatg atggactatc tataaattct tttattacta aaataaaaga
     4561 aaaatatcct catcagttat ttatggcaga tatttctaca tttgaagaag gcaaaaatgc
     4621 atataaagca ggaatagatt ttgtaggaac tactttaagt ggatatactg aggaaagtaa
     4681 aaaacaagat gggccagata tagatttgat taaaaaattg gtagaagaag ggatttgtgt
     4741 aattgctgag ggaaggattc atactccaga acaagcaaaa gagataaaaa aaataggagt
     4801 tgcaggtatt gttgtgggtg gtgctattac tcgaccacag gaaattgcat caagatttgt
     4861 taaagcatta agttaggggg agaaataaaa tgtttaaaaa attttcacaa ttaggacgcg
     4921 ctttcatgtt accaattgct atattaccag tagccggatt actattggga ctgggaggag
     4981 cgttaacaaa tgagagtgct ataaatgctt atccattttt agatcaaccg tggttgcata
     5041 caattttaag tattatgagt tatgctggaa atgcagtttt tgcaaaccta gcactaattt
     5101 ttgcaattgg tattgctgta ggacttgcaa atggagataa aggaacagct ggtttagctg
     5161 gtggagtagc ttatctagta tatactgcaa ctattagtgg gtttcttgaa cttttttcag
     5221 caaaagatgc aacactagat acaggtgtag tgggatcaat tgttgttggt ggtactgttg
     5281 catttttaca caatcgttat agaaaaattg aattaccaca attcttaggt ttttttggag
     5341 gatcaagatt tattccgatt gtttctagtt tagctgcaat cattattggt tctttctttt
     5401 atttaatttg gccaccaatt caaaatggtt tagtagttgc tggtgaacaa attgcccaaa
     5461 tgggtagttt aggaactttt ttatatggtt ttttattgcg tttaacaggt gctgttggac
     5521 tacaccacac catttaccct ttgttctggt atacatcttt aggtggtacc gaaactgtag
     5581 ctggatcaac aattgctgga gcccaaaata ttttctttgc ccaactagct gatccaaacc
     5641 acactggtct gtttacttat ggtactagat tttttgcagg aagatttgca acaatgatgt
     5701 ttggattacc agcagcatgt tatgcaatgt atcgtgcaat tcctaagaaa aatcgtaaaa
     5761 agaatggtgg gctttatttt agcggggccc ttacatcatt tttaactgga attactgaac
     5821 cagttgaata tatgttttta tttgttgctc cttggcttta tgtgattcat gcattcttag
     5881 atggattatc tttttatttt gctgatatct taaatattcg tgtagggaat tccttttcag
     5941 gtggtttgat cgacttttta ttattcggtg tacttcaagg gaatgacaaa actaattggt
     6001 taaaattaat tccttttggt attgcatggg catttatata tttctttgtt tttacattct
     6061 ttatcaaaaa atttaaggta gctattcctg gaatggaaga agaatcagaa gttcattctg
     6121 aaaaagtaat aaacaaaaat acatcattac atgaagagtc tcttatcatt attgaagctc
     6181 ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc aactcgtttg cgtgttgctg
     6241 ttacgaatgg ggagattatt caaaaacaaa caataaaaga cactggtgca actgctgtct
     6301 tcgaagtgaa gggtggcatc caagctgttt ttggagggaa agctgactta ttaagtcaag
     6361 aaatcaatca aatattagga aaagataatt aggaggtttt taatatgttt gaaattttca
     6421 aaaagaaaaa acaagaaact ttttatgcac cttgttccgg aaaaatcatt tccatcacag
     6481 aagtaaatga tcctgtcttt tcacaaaaat tactaggtga tggttttgcc gtcatacctg
     6541 atggttcaaa aatttatgct ccattggctg gtcgagtaac aaatatattt cctactaaac
     6601 atgctattaa tataaaaagc aactcaggca ttgattattt gattcatatt ggaattgata
     6661 cagttgaatt acaaggagag cctttttcga tttgtgttca agaaaatgat gaggttactc
     6721 ctgaaacctc gttggttgat gtggattttg acaaaataat aaaatctggt aaagatactt
     6781 ctgttatagt tgtttttccg gagaaaaatc agttcaatga tttaataata gataatggtc
     6841 gaattgctca tgggaattca tgtggaaaaa tcacttttta aaaaagtgta tataaaaagc
     6901 tataaaataa gctaaatagg atatttgtca aatgaagttg gtgaacagaa agaactgttt
     6961 tctttctgtt cattttttta ttttattagt aatgattccc catgccaaat catgatccgt
     7021 aatttaaagt tataaaagct tcgaaatcca ttggcatttc gttggaatac cttgatatga
     7081 ttattcaaat ataaaatata gagagaagag agagagggaa gatatttata aagggcaaag
     7141 ttatgtttac tcaacttagg aaatagaaat agatcaattt atattctctt gtcaatcgaa
     7201 tatgaacctt agcactatat accaaatata gtaaaatgct gaaagatgt
//
LOCUS       DANKAY010000125         7218 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000125 DANKAY010000000
VERSION     DANKAY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(230..907)
                     /gene="phoU"
                     /locus_tag="PWY98_002569"
     CDS             complement(230..907)
                     /gene="phoU"
                     /locus_tag="PWY98_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2038276.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(920..1678)
                     /locus_tag="PWY98_002570"
     CDS             complement(920..1678)
                     /locus_tag="PWY98_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2038277.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="PWY98_002571"
     CDS             complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="PWY98_002571"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2038278.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="PWY98_002572"
     CDS             complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="PWY98_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2038279.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="PWY98_002573"
     CDS             complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="PWY98_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2038280.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4434..5291)
                     /locus_tag="PWY98_002574"
     CDS             complement(4434..5291)
                     /locus_tag="PWY98_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2038281.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5628..7076)
                     /gene="cls"
                     /locus_tag="PWY98_002575"
     CDS             complement(5628..7076)
                     /gene="cls"
                     /locus_tag="PWY98_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2038282.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2281 t
ORIGIN      
        1 tttgcattct ttatcctttc tatgaaatga tgaaaagggt ttgtgacatg ttttgtcaca
       61 aaccctttcc cgaataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa
      121 acgaaaaata tgaggagcta ttttttgttt gactgttctt attacttgat gtagaacagc
      181 tttttcacaa cctcttttca taaacaaacg gcataatcac agcttcagtt tagaaaatct
      241 cattgtggtt cgtatttaac tcagtgattt tccctgtagc caagtagacg atccattcac
      301 agatgtttgt cacatagtct ccgatccgtt ctagataact ggcaacattc aagtagtctg
      361 ttccactgat gactgtctca ggattttcct gcatattttt gattgttgca ttatagattc
      421 gattgaaata gtcattgact ctttgatcca tattggcgat cgtacgtgcg tctttttcgt
      481 ctgttttgac ataagcaact aggacgttgt ccaccatttt tttcacataa tcggacatat
      541 cggagatctc tttttcgatt tccggtattc tcgtctgccc ttttacgcga atcgtggatt
      601 tagcaacaga caccgcatga tcggccatac gttctaaatc cgagctagct ttcatgaccg
      661 taatgatcat acgcaaatca gttgtaactg gctgttgcaa tgcgatcatt tcaaaacttt
      721 tcttctccaa gcgtgtttcc atgttattga tatttatatc gttttcgata acttcttggg
      781 ctaagatttt atcgtgtttg atgtatgcac gaactgactt gtgcacggcg ctgctcacca
      841 tcatccccat ttcgtagaac tgattatgga gattcagcaa ttcttcttca aattgtgtac
      901 gtagcattcc gtttcctcct tatccaaatc gacctgaaat ataatcttct gtctcttgtt
      961 tttctggatt catgaatatc ttcttcgtat cgttgtattc gatcaagtga ccatctaaaa
     1021 agaatgccgt cttatctgaa atacgtgaag cttgagacat attatgggtc acgatgatca
     1081 tcgtataccg ctctcttaat tctaacagca tactttcgat tttcccagta gaaacaggat
     1141 caagcgcact tgttggttca tccaataaga tgatttccgg attaactgcc aaaacacgtg
     1201 cgatacatac acgctgctgc tgcccgccgg ataaagataa agcactttta tgcaatttgt
     1261 ctttcacatc atcccaaaca gacgctgcct tcaggctttc ttcaactact tgatcaagga
     1321 cttttttatc tttttcccct tttaatttca atccatagat gacattttca tagatagaaa
     1381 aaggaaatgg gtttggctgc tggaatacca tgccgatttc ttttcttagt tcaacaacat
     1441 ctgtttttgg accatagatg tctttgtctt tatataaaac acttccggtg atcgttacat
     1501 tcggaatcag gtcattcatc cggttcaaag aacgcagata agtggatttt ccgcaaccag
     1561 aaggaccgat catcgcagtg atttcccctt gattgaaact tagatcgatc ccttttagtg
     1621 cttctttctt tccataatac agatgaagat ctttagatga aataatttct tttgtcatgt
     1681 tgaggcctcc gtcttaacca aaatgaccgg acacataatc ttcggttgcc tgaatttttg
     1741 gtctggtaaa tatcttgtta gttttgtcat attccattac ttttcctaga tagaaaaagg
     1801 cagtatagtc gctgatacga gctgcttgtt gcatgttatg cgtcacgatg atgatcgtat
     1861 agtcttcttt caagtttaca agtgtttctt caaccgttcc tgtcgaaata gggtccaaag
     1921 cacttgccgg ctcgtctagc agcaagatat caggtttcat cgcgatcgca cgagcaatgc
     1981 ataaacgttg ctgttgcccg ccagataaag ccaaggcact tttgtccaag ctgtctttta
     2041 cttggtccca caaggcagct tgtttcaagc ttgtttcaac gatttcatct aatttctttt
     2101 tgtttttttc gccgtgctgt ttcaaagcaa atgtaatatt gtcataaatc gacttgctga
     2161 acggattagg acgttgaaaa accatgccaa tccgtttgcg catctcataa acatccactt
     2221 gaggcgcatt aatatctacg ccttgataca tgattttccc agtgattttc gtattagcga
     2281 tcccatcatt cattcgattc aacgaacgaa gatacgtaga ttttccgcag ccggaaggac
     2341 caatcagtgc agtgatctta tttttttcaa attccagatc cactcctttg attgcttcat
     2401 tgtcgccata ccagacatgt atatcatccg tatatagtgc tacttctttc tcttttggca
     2461 tggtgatgac atttgtttca ttccagttat attttttcat tttgcttctt ctccttaggc
     2521 tgaagtcatt tttcggtata atcgtttgcc aatcgcacga gcgccgaagt tgaacagtaa
     2581 aacagcaatg atcaacacgg cagaagcgcc agccgaaaca gaaacaccat caggcatcgt
     2641 gccttctgta ttgatcttcc aaatatggac agctaatgtt tcagcttgac ggaaaatgct
     2701 gatcgggctt gaaacactta atggattcca gttcgtaaaa tctaacgcag gtgcactttg
     2761 tccagccgta tagatcaatg cagctgcttc accaaaaatt cttccagaac ttaggatcac
     2821 accagtcaaa atccctggtg tcgcttctgg aaccaccact ttcatcactg tttcccagcg
     2881 tgacaaacct aaggccaatc ctgcttctcg ttgtgtgtaa tggaccgctt gtaaagactc
     2941 ctcaactgtt ctagtcaaaa gaggcaagtt gaatacagtc aaagccaatg caccagaaat
     3001 aatcgagaaa ccatactgga attgaataac gaaaaccaag aaaccgaata aaccgacaac
     3061 gacagaaggc aacgagctca agatttcaat cgacgtgcga atcacatctg ttagccagtt
     3121 ttttccagca tattctgaaa gatagatacc cgcacctaaa gaaataggaa aactgatgat
     3181 catcgtgatc aaaagcaaat aaatcgagtt aaatagctgg atcccgattc caccaccttc
     3241 ttggtaggct ttggaaggtt cggtcaagaa ggaccatgaa atgtgcggta ttccccgaac
     3301 taagatataa agtagtaaag ctgctaaaat caatacgatg atccccgaaa ctatatataa
     3361 aacacctgta gcaattttat ctgagcgttt tgcgttcatt atttcagctc tcctttcttc
     3421 ccaatcatac ggatcacgat attaaagaat aatgacatca atagcaagat cagcgctaat
     3481 gaccataaca cattattttc aactgttccc atgattgtat ttccgattcc cattgttaag
     3541 atggacgtca atgtagaagc aggagtagta agtcctgttg gcatcaatgc tgcattaccg
     3601 atgaccattt ggatagctaa ggcttcacca aacgcacgag ccataccaaa aacgactgct
     3661 gttaaaatcc cgggcacggc tgctcgtagc acgactttgt agattgtttg ccatcttgtg
     3721 gcaccaagcg ctaatgaagc ttcccgatag tgccgtggta ctgcctttaa tgcatcaaca
     3781 gtcatcgttg tgacagtcgg caagatcata acgaacaaca caaatgtacc agctaagata
     3841 ccaaaccctg tcccgccaaa aatacttctg acaaaaggca cgataactga taaaccgata
     3901 aaaccataaa cgacagaagg aatacctaca agcagctcga tgaccggctg taagatcttc
     3961 gttccgtttt taggagagat ctccgtcatg aatactgctg caccaatggc aaatggcgtg
     4021 gcaacgatag cagataaaaa ggtcacaata aacgaacctg tgatcatagg caatgcccct
     4081 accattggtt tcccatttgc tccgatttgg cttggattcc aatctgtacc aaacagaaaa
     4141 tcaaatacat tgatcttatc agtaaaaaag gtggccaatc ctttactagc tacaaaataa
     4201 aaaatagata atacaaccaa cacgatcagc gcgatacata aaaaactgat gaattttcca
     4261 cgctgttcca tttttgaacg ttttgatttt gttaataata cttttttcac atcttccaca
     4321 gatgactccc ccttaatcag gtttaacata ctcactcagt gtacaaattg aacatcaatt
     4381 tggttttaat aacatgtaaa agtttagcta agtttgtaaa ttttgtgtaa agattactct
     4441 ttaacgatat ttccttgcca atcacgttcg actttcatat gagaaacagg gatataacct
     4501 agttctccaa cgatgttatc ttgtacttca tcagataaga tatagtctaa aaattctttt
     4561 gtcagttttt ttggatgatc tcttgtgtac atatgctcat atgcccaaat cgtccatttg
     4621 ttcgttgtca cattttcatc tgtcggttct acaccgttaa cattcaacgt atctacatca
     4681 tcggaaacat aagaaaaagc aacatagctg attgcaccgg gtgtgtcggc aacgatctgg
     4741 cgcaccattc cgcttgaatc ttgttcttgt gcctgaacag ctgtttctcc gtccaatacc
     4801 catttttcaa acgtgctacg ggttccgctt cctgaagcgc ggttcaatat aactactggc
     4861 tgatcttttc caccgacttc actccagttt ttgatctcgc ctgtaaatag ttttttcagg
     4921 ttttccatac tgatgtcttt tacgccaata tccttgttca caataggagt gatcccaaca
     4981 actgctactc gatgatcgac taatgctgag gcatcgatcc ccgatttttc ttccgcaaac
     5041 aaatcagagt ttccgatgtc gactgctcct gactgtactt gagacaaacc agttccgctt
     5101 ccgccccctt gtacattgat gaattttcca ggattttctg tctggtaagt ttcagcaact
     5161 gtttctacta aaggctgtaa agcagaagag ccaacagccg taatggattc tcctcgatca
     5221 atccactttg aacagcctga taaaagaatg gctaatccta ttattggtaa gaataattta
     5281 agttttttca ttagaaatct cctttaatcc taaagtatgt ttattttatc attttcatta
     5341 cttgatgaaa acgaatcaaa atcattaaat aacaaaataa attctcataa ttgtaaaaaa
     5401 aactatctca ttaaaagttt ctatgctata ctggctttga aatgtgtctc ttttaatagc
     5461 atatattagc ttatttcttg ctttccccaa aaaacaagag ataccccaaa gttaatttcg
     5521 ctcatgcgag acgctttctc catttctgac gtactctcag aaatggcccc gttagaaaag
     5581 gacacgagat ctccatctgt tgatggttct catgtccttt ttatgtttta aaggagcggc
     5641 gaaatcaatc ttgctaatcc ttctttcatc ttgatgatta ttcctcggtt ttcatacatt
     5701 tcttttgtga gatgcgtaga aattttcgag tcttcaaaaa aagcttttct tacacgtgaa
     5761 gccattcgtt catcataaac cagtgtattg acctcaaagt ccaagcgaaa tgaacgaaca
     5821 tcgatatttg ctgagccaac tgaaacaatg ccaccatcga taatcatcgt ctttgcatga
     5881 ataaatccat tctcatatgt ttcgatcctt gctccatact cgatcagctc tgcagcgaaa
     5941 gaatacgttg cccagtaaac gagaagatga tctggtttgt taggtatttg gatatgaact
     6001 tttacgcctg aaagaagagc caacttcagt gcttcatgaa tcgatccatc cggtatgtaa
     6061 taaggcgttt ggatcaatat ctcccgtttg gctaacgaaa tcattttcaa ataagtcatt
     6121 ttgatttgtt catgttcggt atctggtccg cttgtcacga tttgaacagc tatatcacca
     6181 gtagtgtgaa tggacgggaa aaatttggga gaatagccta cttcttttgt atgctgagaa
     6241 ttccagtcca tcaaaaaacg gttttgcaag gtatacaccg cttctccgta aatacgcaaa
     6301 tgattgtctc gccaataacc aaatttcttt accaatccta gatattcatt tccaacattg
     6361 aacccacctg tatatccgat tttcccatca ataactacga ttttacgatg attgcgataa
     6421 ttgatccgag gattaatata cggaacaaat agaggaaaga aaaatgcgac ttctccgcct
     6481 gcttttttca aattctgaaa aaagctggaa gaaacttgtg tggaacccca tgcatcaaga
     6541 aggactctga cttttacccc tcgtcttgcc gcctctgtca atgcatctcg aacttcttca
     6601 cctaatgcat cactgcgata aatataatac tggaagtgga tatggtcttc tgcctgcttg
     6661 atatcttcaa tcagcgcatc gaatttttca cgaccatctg taaataaagt gatttcattt
     6721 ttagtcgtat agagcgtact ttcaaatacc gtgagcatat agatcaattg tttcacatct
     6781 acttgtccag taggcggatg aggataaagc cctcgcatca atgcctgttt gtcttcttca
     6841 atttccaggt tcttaccgat tttcccttgg atcttcaaat caaagatttt ctctttggaa
     6901 atccctcttc ctaaaaaaat atataaaata aatcctacaa ctggaatgaa catcaaaacg
     6961 agcagccatg cccatgtttg agcagtttcc tttctttctc gaaaaacgat gataaaggaa
     7021 agcaaaatat tcataatcaa aaggatcgta aaaaaattgt caacgataat tttcacggct
     7081 tcacctcttc ttctaccaac ttaagaatac ccaagttgtt ttttaaagta aagggaaaaa
     7141 agccgcttcc ttctattctc ctagccaata aaaaaagaaa gctggaacga aactgtccaa
     7201 ctttctcatt ttatttat
//
LOCUS       DANKAY010000126         7176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000126 DANKAY010000000
VERSION     DANKAY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(969..1142)
                     /locus_tag="PWY98_002576"
     CDS             complement(969..1142)
                     /locus_tag="PWY98_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038283.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1145..1543)
                     /locus_tag="PWY98_002577"
     CDS             complement(1145..1543)
                     /locus_tag="PWY98_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038284.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1759..2814
                     /locus_tag="PWY98_002578"
     CDS             1759..2814
                     /locus_tag="PWY98_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDL2038285.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2834..3187
                     /locus_tag="PWY98_002579"
     CDS             2834..3187
                     /locus_tag="PWY98_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038286.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3369..3665)
                     /locus_tag="PWY98_002580"
     CDS             complement(3369..3665)
                     /locus_tag="PWY98_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038287.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3669..4487)
                     /locus_tag="PWY98_002581"
     CDS             complement(3669..4487)
                     /locus_tag="PWY98_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2038288.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4713..4934
                     /locus_tag="PWY98_002582"
     CDS             4713..4934
                     /locus_tag="PWY98_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL2038289.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4936..5202
                     /locus_tag="PWY98_002583"
     CDS             4936..5202
                     /locus_tag="PWY98_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2038290.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5315..5854
                     /locus_tag="PWY98_002584"
     CDS             5315..5854
                     /locus_tag="PWY98_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038291.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            6063..6245
                     /locus_tag="PWY98_002585"
     CDS             6063..6245
                     /locus_tag="PWY98_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2038292.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6348..6632
                     /locus_tag="PWY98_002586"
     CDS             6348..6632
                     /locus_tag="PWY98_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038293.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6648..6884
                     /locus_tag="PWY98_002587"
     CDS             6648..6884
                     /locus_tag="PWY98_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038294.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6906..7160
                     /locus_tag="PWY98_002588"
     CDS             6906..7160
                     /locus_tag="PWY98_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038295.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         2492 a         1188 c         1106 g         2390 t
ORIGIN      
        1 cgtgttaagt ttctttacat tcctacgcac cttgcgtgta aaatttcttt attcctccca
       61 cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa ccgaacaagt
      121 ttttacctat tcccaaaatt aatttattcg ttattttttc attgattaag aataattatt
      181 tgatgcttaa attagctaaa aaaacgattt tttactgata taatcatgat attaaagctg
      241 aattaggcgg aaagataaaa tgttcgcaaa atcaaagtat caattatctt gtttttttat
      301 aaatttgtaa gtaaaaatct aaaaagtttt cttttaattg gtatatgtat taaaatcaca
      361 ttcaatcact cataaaagta tgcgctttca ttttttttgg atgagattaa tatcaataaa
      421 taacgatgta aaaaccatta attatgctct tttataagga tattaattaa tctaatttga
      481 aaaaataatt caaaatttaa atgttttaat attacaaata aaatcatgtt atcgtaaaat
      541 ggtataaaaa agctaaatat tctatttttg ttattttatt agcagggaaa tttaaatagt
      601 ttacaagaat ccgtagaaat atattggttt tgccttcaga ttaatactgg atcatattaa
      661 tctgaatata atatatgaac taataaatag cttaacctcg ctattttgtt ttttctgatc
      721 actatctata cagcattagt aaatagaatt ttaaaaataa aaaaattaag acaaaaaaag
      781 tatcaacata aacatataca tatacacgtt tatgttgata cacttaattt gtcctttaaa
      841 aagcacttat tttaatatta tgttatttta taatcttatt acaagctgat tgttagacgt
      901 tgtcctttca ttacatttat taacggttat tttatcgttt aatttattaa ttatttttga
      961 tctaaaactt agcttaacaa ataaataatt aaccaaccgc cagataaaaa tgcgatcatt
     1021 gctaatacat aagcaaagat aaaaatatgt tcgatcagct taaaccctga tgcgattaag
     1081 cagaccaagt aaaatagaaa ttctcttaca gataatggta attttctaac tagcatactc
     1141 ataattaaca ccccaaagta atattgtatt ttgaacattt tacatccaat acagcatcaa
     1201 tcaaccattg ccaatcaaaa atacaaggct tcattgaagt atgtttttca gatgaaaaga
     1261 tataatataa tttcttcgga ataatatttt cagttgttgg gatcaagcta aatttaatac
     1321 catctaactc atagctacca gctttctctc cattaaaaat ttccttagca ctttcttcta
     1381 aagaatcaaa atatttttcg acagcaacct ttgaagcttt tactattgaa gcatttccct
     1441 tttcgataaa atctgctttt gttttaccga caataagtaa ttcttgatca gtaaaatctt
     1501 ctaaaataat agtagtgtaa ttcttatttc ttctcaatcg catcaggatc actccttaat
     1561 ataatttatg cttttataat atcataataa gataaatttg caagtgttta tttacacctt
     1621 tttatttaca ctttattgta attgattata cttttttgat tgcatttttt tcttataaac
     1681 agttgcaact attagtaaat aggtgtaaac tattttcgta ccgaaatacc ataaacacaa
     1741 acagaaagag gtcataccat gaaagaacca ttattgtttt ccgttgactt agggaataaa
     1801 tcagtaaaag aaatgagtac acacgtttct ccactctcgc ttccctctcg tattttgaac
     1861 aaaaatatca ttaattcagg acgtttttct aatataggtt cctcattaaa acgatcacta
     1921 aacgtttctg aatatagcct agtaaacact actgaaagct attattttgg gaaggatata
     1981 cacgagctag gaaaagatga ctacgtgata gaatcactag gttttgggat cagcagatac
     2041 aagcaggaaa cctaccaaga tatcctatct ttctctttag ctaacctagc acgtttagaa
     2101 gatcaatctg atgacgtttt aaccgttaat ctagttttag gtctgccttc atccgactat
     2161 accgaaacaa tcgtcaaata cgtgatgcaa caagccacac gacaacattc agttgaagtt
     2221 gacggtaaag cttataatat aaacgtagat accgtttggg tactgccaca gccgattgga
     2281 acactatata acctcctcct tgctgatgat ggtagtgtac aagatgaatc attgatcagt
     2341 cagaagatcg gttttgttga tattggtggt ggcacgaaac tttttgatgt tgtccaaaat
     2401 tttgaaattg acgaaaacaa ccgtgaacaa aaggataccg gtatttttac tttatataat
     2461 cgtatcgtaa atatgcttga ccttccgatc agtcctaatg ctttccaact tgaaaagatt
     2521 atccgagaag gcaaagaaaa aggcgtatat ctatttaaac caagcggaaa tgaaagtttt
     2581 gatatttcag aagtagttga aagagaaata aaaacataca ccaatatggt tgttgcagaa
     2641 gttttagcta cattcaaaaa cttcacaacc ttagataaag ttgtctttac tggtggtggg
     2701 gcttctatta tcgacaaaca acttgtaaaa gataaaattc cgcatacctt gtttgttgaa
     2761 gacagtgaat tttctaatgt aaaaggcttt tacaagtttg gtttaactca ataaaacagc
     2821 gaaggagatc aacatgaata agggaaaatc agagcgttat gtagttataa tcaatagaga
     2881 aaaggttagc gagttacctt tagcagagtt tcttgaatct gaatatgaaa cactaggcgt
     2941 taaatactct cagacgatca aaaaagcttt gaatctatat cggcgtgtaa agaacaatga
     3001 agttacaatt attgaagcag gcaacgatag aaacgatagc aaagtagctg aagtaaagaa
     3061 taaaccaact gatcataacc ctattcaaca agcgcatcct tcaacggtta catccgatca
     3121 ttcagcaaag aaagaaaagc caatgctaaa agggttcaat gcaccaagta acaaagactt
     3181 actttaaaaa taaaaaggcg aggttacaat acctagcctt tttattatgt acaaatacaa
     3241 acataaacat atacgtatac atgtatataa acatgtatac gtatatatcc ataaacacgt
     3301 atacgaaatt tagttaagct atacctaacc gcttattcca ataaaaaaag tgatcagctt
     3361 taaactgatc actcaccaaa aacttcatca tacaagttat ctatcatttc accaagtaaa
     3421 gctgaatctg atcgaattcc taacttttcg ctgattttag atagtttctt tctttgtgat
     3481 ggttgaagcg tgaattgata atttttcttc ttttcagaac tatcactttt tagtttagct
     3541 accaaagaac ttgttgtttc tttaggtgtt tgtttaggtt cttcaactgc ttcaaccact
     3601 tcaggaactt cttctacttc agcttcagcg aattttttaa atctattttc tttcacttca
     3661 atcactcctt attcttgtgc cgtatcaatt attttgttgt atagcgtaaa cgtgctttca
     3721 aagaaatcct tattcttacg atagatattc ttatccttag cgaactcagc aatattctta
     3781 ccttctaagc tagttgcatt aagcaattcc ttctcttgta catatccaag ataattagat
     3841 aacgttggta ccaattctag gaactcacga cttgaattag tattatgttt gatcctgcta
     3901 ccgatcgtag cgtatttagt ttccatcagt gtttcaccag tcttaatatc aatcaactgt
     3961 tttgctagtg catcaatatc tttaccaagc ttaagcaaag cttcaaaacc atctttagat
     4021 ggatcagaaa tgcctacgac aagatcagat acagcccaag cattttgagc tagtagacct
     4081 gcatcattat gagtatcaat cagaattaga tcatacttct ctttaagctg ttcatagtta
     4141 acaacgatcc atctaaacag tagggttctg ttgttaggca tatcacgcat ttgcatctca
     4201 acttgtttta aatcttcacc tgctactaaa atatccatgt tctcatttat agctaaaggt
     4261 tcagctgatc catgaagata taaatgattg attgtattct ctcttttcgc tttactgaaa
     4321 gcatcaggaa atcgttttga taggttttgc gacttatctt catcaatata cagcaccttc
     4381 atacctaaat tttcgacagc atatccacct agattgtaga ttgttgttgt tttacctgtt
     4441 ccaccttttt caatgtttcc agtaatgatt gacaacttct tttccatgat taaacctccc
     4501 ttattaatct tttgtaactc cattatacac tataaacacg tttatgtata tatttatata
     4561 cataaacgtg tttatattta tatttgcttt tatattctac aacataaaca tatatatgta
     4621 tatgtttatg tttatgtata tattcaaata tataactatt gcatttgtca tacaatcgta
     4681 gtacaatgta attgaaaagg agttgataaa ctatgccagt aataagtatt cgtgtatcta
     4741 aagaagaaaa agattttctt gaaaaagttg ctgaattcaa cggagataac atttctgaat
     4801 ttatgcgtag taattccctg caaacagcag aaacgttagt agactttgcc acctacaagc
     4861 aactaatgaa agagcatgag gaaaacgatc aaagtatttc ccacgaagat atgctaaaag
     4921 agttaggctt ataagatgac ttaccaagta agatatgaac gtgaagctca aaaagcgcta
     4981 aaaaaaatgg ataagtttca ggcaaaggtt atcctaaact ggatcgagaa aaatcttgta
     5041 ggcactgatg atccaagaaa gcatggtaaa ggtctaacag ctaataaatc aggctactgg
     5101 cgatataggg taggtgcata ccgtataata gctgatatta gcgagcatga agtgacaatt
     5161 cttatattgt ctattggtca cagaagtgaa gtttataagt aacagacaag ctgataaggc
     5221 ttgtctgttt ttttgttaac ccaacaaaat cactgtttac gatcccactt tacctaaaaa
     5281 agaacaatat cctcttgata ttatacacac cagtgtgtat aatataagtg taaacaagct
     5341 aattaacttt caaaaggagc gattttttat gggatggtat ggtgattatt catcagcaaa
     5401 tgaagtgatc gaagaaaaga aagggtacgc caaaaatggt ttggaaatcg taggtaccaa
     5461 agtaacgaag acctatggaa ttatcctttt caaagatgca gaaggaacct acgatattga
     5521 cagcttcatc tttcgagatg aaatgtacaa accaatctac ttcttaaatg acagtcctgc
     5581 ttactttaag aaaattccga aaaaatggtt gaaattcttt gtcggtgatc aagaaaaaat
     5641 ggttaagcta caactagata gaattaaaga agaaagcaat aaaccaaaac ttgatgactt
     5701 attagttgca ggtaaaactt actcaatctg ggatggatcg tatgtcgctg tctatcagta
     5761 taaactcaaa cgtagcttta tttttcgact acaagatgga tcactcactc ggttcactcg
     5821 actatcagca ggagatgtta aggaagtaac ctaaaactta aatacataaa cataaacgtg
     5881 tatacgttta tgtttatgta tatgtatatg aatataaacg tattaggtac gaacactttt
     5941 aaattctttt ctgggctttg tttttagggt agggctaaaa aaaacatcac catctgatcc
     6001 aaaacagctc agaaacgcat ttaaacgtat ctgataccaa tattacagaa ggagtgtatc
     6061 gtatgattaa cctatttctt ttagtgacag ctagtacatt attctttact ggaatatcac
     6121 tgataatttc ctatttgatc tataagaaag ccgaaaatca cacaagcaaa gcaatcggac
     6181 tagccgtttc atccttttta actttgctga ctttatttgt tactttatcg ttttcttttg
     6241 tttagaacag ctgatccatt agcacctttt tctctttaca ttatacacac tgttgtgtat
     6301 aatgtatttg taaacaagct aattaacttt taggaaggag tgacaagatg ctaacagcta
     6361 ctttacagca acctactcac gttgctattg cagaaagtta tacaattaat gcagggatcg
     6421 ttaaacagct gatcaaggat cgagaattca aaaagtttaa cagtcggcaa gcgtataaca
     6481 gacagctaaa gaaagctatt ctaaagcaca aagattcctt catcgggaac ttggaaagtg
     6541 tagatgttaa aactgtttca acggcatttt cagttgttga agtgactatt gacggtcgct
     6601 tgatttctct atttgctttt ggaattaatt gatagggagt gtgaactttg gaaggtaaga
     6661 cttttgcaga aatttggact gaatttattg aggtaccaac tcgcattgat ccgataactt
     6721 ctgaaaaggt attggatgca gacttctaca atctcaaagc aggtactagc cttgaatgcg
     6781 tttggatgta tttagaagag catttcaaag gcgtaacgct cggtcacgtc ttataccaca
     6841 atggtgccga gtgggatgca attggaaata tccatttttc ttaatataag aaaggagaac
     6901 acaatatgaa ttataccgat gaagaacaaa tcgctttgga atacgttttc acaaatgaag
     6961 aaggggagga aattgaaaaa gtcttgatca tttcatcaaa agacttggag aaggctttgg
     7021 cttatgaacc atacgaatca accgtaactg tcattgaatt cttagatacc tatacttcta
     7081 atgatacttg ggaaattatg aatatttgca acgctaataa cattcaattt gctgaaaaaa
     7141 ccaataaatc attttattag ttttgcaata aaacat
//
LOCUS       DANKAY010000127         7065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000127 DANKAY010000000
VERSION     DANKAY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            6..653
                     /locus_tag="PWY98_002589"
     CDS             6..653
                     /locus_tag="PWY98_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2038296.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            737..1681
                     /locus_tag="PWY98_002590"
     CDS             737..1681
                     /locus_tag="PWY98_002590"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2038297.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1816..3267
                     /locus_tag="PWY98_002591"
     CDS             1816..3267
                     /locus_tag="PWY98_002591"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2038298.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3288..4124
                     /locus_tag="PWY98_002592"
     CDS             3288..4124
                     /locus_tag="PWY98_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2038299.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4124..4783
                     /locus_tag="PWY98_002593"
     CDS             4124..4783
                     /locus_tag="PWY98_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2038300.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4909..5598
                     /locus_tag="PWY98_002594"
     CDS             4909..5598
                     /locus_tag="PWY98_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038301.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5604..6560
                     /locus_tag="PWY98_002595"
     CDS             5604..6560
                     /locus_tag="PWY98_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2038302.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6604..>7065
                     /locus_tag="PWY98_002596"
     CDS             6604..>7065
                     /locus_tag="PWY98_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2038303.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIE"
BASE COUNT         2325 a         1265 c         1504 g         1971 t
ORIGIN      
        1 aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt
       61 gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct
      121 cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa
      181 gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat
      241 tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg
      301 gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg
      361 atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa
      421 atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag
      481 cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata
      541 aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag
      601 tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc
      661 ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt
      721 tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc
      781 tttgatgaat gcatctggtg tccactgcat gactacccag gaattagatg aactggctca
      841 ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc
      901 cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt
      961 aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa
     1021 tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga
     1081 gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt
     1141 gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt
     1201 attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca
     1261 ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa
     1321 cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa
     1381 agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg
     1441 cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac
     1501 aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga
     1561 gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat
     1621 gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata
     1681 gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt
     1741 cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc
     1801 aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa
     1861 tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa
     1921 ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc
     1981 cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa
     2041 aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat
     2101 acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat
     2161 gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac
     2221 ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca
     2281 tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct
     2341 tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat
     2401 gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac
     2461 acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg
     2521 ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat
     2581 tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat
     2641 ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa
     2701 ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt
     2761 gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg
     2821 ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat
     2881 gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg
     2941 actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag
     3001 atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt
     3061 gaggagagct tgaagatcac accttataca cagttggcta atctgactgg acaaccagca
     3121 atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct
     3181 gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag
     3241 ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa
     3301 gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg
     3361 ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga
     3421 cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg
     3481 atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat
     3541 acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg
     3601 attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt
     3661 catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc
     3721 ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac
     3781 acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag
     3841 caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg
     3901 ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg
     3961 gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag
     4021 agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt
     4081 cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcccctaat
     4141 tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt
     4201 agggattggc ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt
     4261 aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag
     4321 aggcattccc gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag
     4381 tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat
     4441 ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg
     4501 tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt
     4561 accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat
     4621 cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt
     4681 ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt
     4741 gcaacagtat ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct
     4801 agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa
     4861 ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg
     4921 ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt
     4981 gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca
     5041 aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt
     5101 cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa
     5161 ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg
     5221 tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca
     5281 aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc
     5341 agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa
     5401 ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat
     5461 tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt
     5521 atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct
     5581 acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc
     5641 tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga
     5701 tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc
     5761 accatccaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc
     5821 atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc
     5881 cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc
     5941 actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga
     6001 taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact
     6061 cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg
     6121 atgactatct ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag
     6181 gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata
     6241 ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta
     6301 aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc
     6361 accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac
     6421 aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta
     6481 ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac
     6541 tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac
     6601 tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat
     6661 gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca
     6721 acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa
     6781 gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata
     6841 gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca
     6901 gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca
     6961 gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa
     7021 aagaccatct attctgctgg attcacttat caagggatga ttgaa
//
LOCUS       DANKAY010000128         6906 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000128 DANKAY010000000
VERSION     DANKAY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            188..1198
                     /locus_tag="PWY98_002597"
     CDS             188..1198
                     /locus_tag="PWY98_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2038304.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1267..2211)
                     /locus_tag="PWY98_002598"
     CDS             complement(1267..2211)
                     /locus_tag="PWY98_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2038305.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2195..4915)
                     /locus_tag="PWY98_002599"
     CDS             complement(2195..4915)
                     /locus_tag="PWY98_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2038306.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4912..6132)
                     /locus_tag="PWY98_002600"
     CDS             complement(4912..6132)
                     /locus_tag="PWY98_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2038307.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6413..>6906)
                     /locus_tag="PWY98_002601"
     CDS             complement(6413..>6906)
                     /locus_tag="PWY98_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDL2038308.1"
                     /translation="KKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDAIS
                     IYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYN
                     LKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERI
                     LTI"
BASE COUNT         2047 a         1305 c         1120 g         2434 t
ORIGIN      
        1 aaatataata atgattattt tctcaacaga tttaactata taagtgagaa aagtttgcgt
       61 atttcctagg aatgtgatga aattttgaat tttgtttgaa actaccacga caacgttttt
      121 aaaggaaatg ggatatggta tagttaatat ataaaaataa taactaatta caggagtgca
      181 aattttaatg aaaaaaaaat caattatttt ggcagctact agtgcgttgg ctgttttaac
      241 tctagcagct tgttcaggcg atactaataa agatatcgca acgatgaaag gcggaacgat
      301 cactgtatct gatttctatg atgaagcaaa acttgaatca tcgaaccaat ccttagtaca
      361 acggatgatc atttataaag tattcaataa caaatatggc gataaagtaa ccgataagca
      421 agtggatgca gaatacgaca aacaagcgaa atcattaggt gatacttttg aatcacaatt
      481 agaagctgct ggatatacga aagatactta taaagaatat attcgtaaca atttagcttt
      541 tgaagctggc ttgaaagctc acgtagatat tacagatgac gatttgaaaa ctgcttggaa
      601 atctttccat ccagaagtag aagcacaaat catcaaatta tcaagtgaag atgaagcaaa
      661 agatgtgaaa aaatcagcgg atgatggaga tgatttctct aaattagcca aagacaaatc
      721 aactgataca gagacaaaag aagatggcgg taaagttaaa ttcgactcaa cgacaacaac
      781 tattcctgca gaagtaaaag aagcagcttt caaattgaaa gacggtgaga tctccgatgt
      841 tattactaca acaaatccta cttcttatgc gacagaatac tacgttgtca aaatggtgaa
      901 aaaccaaaat aaaggcaatg acatggataa atacaaagat caactaaaag atatcgctac
      961 tgaaactaaa ctaagtgaca atgcattcac aactaaagta atcggtgaag aattaaaaga
     1021 tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg ttgagtgcat tcacaacaac
     1081 ttcatcaagt acgaaagact caagtgaaac tacagcttca acaaagagtt ctgacacaaa
     1141 atcaactgat tcaacaaaag aatcttcaac tgaagaaaca actgattcat caaaataaaa
     1201 gaaaacaaaa aaagcgaaga cttatgtctc gctttttttg ttttgaatga aaaaaataaa
     1261 tttttgctat aaatcttttg gtacatagaa acttcggttg tccataccga aaatccgatc
     1321 ggtgtatttt cctggttctg tttgacgaat cgaagtcaac atcatctgca tcgaagcatc
     1381 gatattgtcg atttgatgta atatttctgc ttccattatt cttggacgta caggtgagcc
     1441 atattctaat aagccatggt gtgctaaaac catatgacgt aatacgagaa catcttcgtc
     1501 agcttcgctg attttcaacg caagacaggc tttagttatt tcttcgtcaa tcaataccag
     1561 atgaccgatc aaatttcctg caagcgtata ttctgtcgac ataggaccgg aaagttcaat
     1621 cacctttcct aaatcatgca aaatgatacc agcatacaac aatgctcgat tcagctcagg
     1681 atattcatcg gcgattgatt tccctaagtg aagcattgtc aaagtatgat aagctaatcc
     1741 actggcaaat gcatgatggt ttctttttgc tgccggaaac tcaaaaaatt gtttttgata
     1801 tttattaagt agatagcgaa caatacgatt ccaatgcgca ttggtgatct caaacaacaa
     1861 ttgattgatt tcttcttcca tttcttcttt cttcaaaggg gctctttcca tgtataaact
     1921 aggctctcct ggctcatcta cccttgttaa gcgcatatga agaatcttta cttgagggtt
     1981 tccttgataa acctctcttt taccgtttag aaaaacgact gtgccggctg taaatttatt
     2041 gatttcctcc tccgaagcat cccagtattt accatcgata gtacctgaag tatcttggaa
     2101 agtaaaagca ataaattttt ttccgttttt agcgattctt acatctgcac ttttgatcag
     2161 gacaaattgt tcaaactgct catcgattac taaatcacga atttttttca ttatttgctc
     2221 cttctaattg ttttacgatt tggttcatct catggtaata gcttaccatt tctttatcag
     2281 aagaaaaaca aattatttga tacttttctc caaatttagc taaaagttca gctaattgat
     2341 actttcgctg atgatcataa tgaagccagc catcatcaat gatgatcggg catatcgaac
     2401 ggttagcttc taatgagagg taagcaaatc tcatcgccat aatcagctga tctttcgtac
     2461 ctgtagataa ctcatagatg ggaaattctg tatatccgtc tgttaaagtc agtgtatcat
     2521 ttttcagtaa tatcttctga taatttcctt ttgttaaaat acctaaataa tcacctgctg
     2581 ctcttaacaa ctggggtaat tgtctttcac tcatctctgt tgaaagatca cctaagaatg
     2641 tcccggcgat ttcaagtgct ccccattcct ccagtaattc ctgtaatttt gctttttgcc
     2701 ggctagcttg ttgataaagt tcgtccaatg tgccgtctct ttgcaactct tctacttgaa
     2761 gttggagtcg ttgccgattt tcacttgctt ctttttcttc ttgctggagt tgggattgct
     2821 tttctttcag ttggcgcagt cttacagata atttctcttt tgtcagctct tcaggaaata
     2881 gtggagtaag catttccgat aattcattgt atcttttcat ctgctgttcg gcttgtttgg
     2941 ctttttgtaa aaagacaggt acttccgaag gataagataa gcctgcttga gtcaattgtt
     3001 cgctgtattc ttgtatccat actttttgtt tctttttcaa ttgattgatt tcttgcttca
     3061 ataaagtatt ttgctgatag cttctggcaa atttcatctg ttccatctct tgtataaaac
     3121 gggaaagcaa taccattttt tctgaaattg tcttatcttg tatcggtaac cattctttaa
     3181 caaactcaaa tttttggtcg atttgtcgta gtttttcttg tatttcctgt tctcttttat
     3241 gtttccgctc aaatacgcca tataatttct gataatctat gacttcctgt ccatattctt
     3301 tcatgagttt gagcgtatcc atctttccta agtgccgatg atccgcaaaa cgatatactt
     3361 cttgctgaag aatctgtttt tcttctccag ttctcttttg tttttcttct gcttcagcta
     3421 attgagctaa ggctagatca agttgtccta gtttttcttg ccacattcct tttatctcag
     3481 agaaatcatt tttagtttta atttttggtt gccttttgta gaagaaatag ccaatcatgc
     3541 ctgcaccaat tagtaaaagg atcactttca agggcattgg tacaaatata cctgcaatga
     3601 ttattgctcc gccagccatc agcatatacg gtttagatga agagactggt gctatctctg
     3661 actgattttg taataatgct ggataggtct cttccaagtc attcacttct gcttcaatca
     3721 tctcttgctg ttctttagct atttggagac gcacagaaat ctggctttct agatcagcaa
     3781 tccttctttc ttcagccaga agatgcttcc attcttcttc atataaaaat tcttccggta
     3841 acggattttt ctgccaatgc cacctttgtt ccaaaatctt caattctgaa tttgtttctc
     3901 tagtctcaat ttgtaatctt tgccattctt cgtttaattg ttccgctgta aattgaagtt
     3961 gctggagatc ttttagttcc tgctcctgct ctaaataaaa ataatatctg gctgactgct
     4021 gatccatccc gctatgcttt tccagtgttg cttctaattc ttccaaacgc tcatttgttt
     4081 gctgataccg tttggagaaa gactccagat ctaaaagaag ttgctcatca ggaagacctg
     4141 tagtgctgtt ttttttgaga gattgccatt cttcatataa agaaaaatta agttcttgct
     4201 gtctggctaa tgaaaattgt ttgttcgcct cttttaattg tgccaataac tgctgctgtt
     4261 gcgactcgta ttcctgttct tgttggatca acttgccaaa ataagcttcc tgttcttgtt
     4321 tttgatgaat tttttcttgc agctctttcc attcatttaa tttttgattg attagtaatt
     4381 tttggccttt tttcttgaat aattgttgtg ctttctgata atattcttgc cgcttttgga
     4441 atacttgaga actgcccgtg atgcctaaag aaatcagcgc atcatgtagc tctttttctc
     4501 tcaaatgatc tagttccatc aactgctctt gctggaaagt aaatacctgc tggaataact
     4561 ctttcgtcaa aggtgcgatc agtttttcta gtaactcatc acttcccact tgatccccca
     4621 gccacacctt cgattttccg cgattttgct gcttgtagcg ctcgacagca aattcaccat
     4681 atacaggatg tttaagccat attttgccgc cataggctga gccgtctttt ggtgtataat
     4741 cctttttttt acggcctttt gaagggaaac caaataatat tgcttgaata aattgataaa
     4801 tcgttgattt tcctgcttcg tttcccccat agatcagctg attgcctgaa taaaagtcaa
     4861 aacttttttg acgccatttt ccaaaagcag caatctcgat ccttaaaatc ttcattcctg
     4921 atcctcctca aataaaaaat cttgattaag cagctgcttg gctttttcaa gtgtttcttc
     4981 ttgatattcc ggcagttcgc tcaatattct agccgcttct tgatgactat atacttcatt
     5041 taaaatttgt tggaaatctt gaggtgtctg atagtattga aataactgtt ccatcaattt
     5101 agcagaagca gcaagtgggg tcttcgtata aaaagtattc tctatgagag aaatacgcca
     5161 aataaagaaa tcatttatcc tatctgagaa ttcctctaac aaatagtcta atagctctcc
     5221 tgattgtatc cgttccaaaa catctgtatt gagatggtca taatctttta attttatctc
     5281 aattaaacta aatccatcct ctacttttga aagctgctga cgaatgtgaa caatgacctc
     5341 ttttgtcgtt cttgctttcc tcaatgatat ttcctttgtc ttccaataga cctctgctac
     5401 tttaatcgga tgtatttggc aacttccttt acttaattca actagtagaa tagatgtaga
     5461 agcttgttcc tttttagtat gaccttgagg cgcacctgga taaacaattg tttgcttttc
     5521 atttaagacc gttggcacat ggatatgccc taaagcccaa taatcgtatc ccttttcttg
     5581 catctttgag ggttggaaag gagcatagtt tcctttttga gagacgccag gttccccgtg
     5641 gtacaaccca atatggtaat ctgtgagttc tctagaagga aattccatca ctttgtcttt
     5701 ttgaatccac ggttgacgat aactaaatcc actgagagag acagtttctc cactttttat
     5761 cgttattttt tttgtctcta ccgtttcgga tgtaaataaa tggacatttt taggaaagga
     5821 gaaccaatac cgctcttttt gatagaaatc atgatttccg aatattataa atacaggtat
     5881 atggttcttt tctaaccgtt tcatctgttc tgaaaaatgt ttctgtattt ttaatgaagg
     5941 acgattttga tgaaaattat ctcctgcaag taaaacaaaa tcaacgttat ttattattgc
     6001 ctgttcaatg atatttgata aaacggtcag attcgtcttc aatagttttt cctgtacagt
     6061 tttatcaaga gttgtcagtc cttcaaatga ccgatccata tgtaaatctg ccgcatggat
     6121 aaatttcacc attaaaaatt tcctcctttt tttcttttat cataccattt tcaatgtttt
     6181 ttttcatcaa taaccgaaaa tttgttcgta ttatgcaaag aaattacgat aaatggccga
     6241 cttcaaaaaa taagtttatt cgaaaaaaag attgtgacat gatttttgtc acaatcctaa
     6301 atatctatgt tatattcgct tcgtttgaca ctgaagaagt actatttgta ctggaattta
     6361 tgctccattt ttccgtattt agaattctat gttctatact accttaatta gtttatatgg
     6421 taagaattct ttcaaatgct tgagatactc cgtctttatc atttgaaaca gtgacataat
     6481 cagctttttt catgatttct tgatgactat ttcccattgc aaaactaata tctgcacttt
     6541 caaacataga taagtcattt aaactatctc cgatcgcgat tctcaaaggt ttctttaaat
     6601 tgtagattga ttccaaacga tcaaacacca cacctttaga tacattttga ggtacgattt
     6661 caatatttac aggtgccgat tcagtaacag acacatttag catctttttc aaatggttaa
     6721 atatattagc tttcaattga cgattaggaa taaaaataaa aaatttgtaa atgctgatag
     6781 catccttttc tataatttcc tcaatattat tataagggat tgaacaaata gatttttcat
     6841 actcaagctc atagtttagc tctttgatag aaaattcact ccctacctcg tgaaaaattt
     6901 ttttca
//
LOCUS       DANKAY010000129         6733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000129 DANKAY010000000
VERSION     DANKAY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..400
                     /locus_tag="PWY98_002602"
     CDS             <1..400
                     /locus_tag="PWY98_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HDL2038309.1"
                     /translation="EDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPK
                     NLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELN
                     KSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            403..1299
                     /locus_tag="PWY98_002603"
     CDS             403..1299
                     /locus_tag="PWY98_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2038310.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1349..1738
                     /locus_tag="PWY98_002604"
     CDS             1349..1738
                     /locus_tag="PWY98_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2038311.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1725..3227
                     /locus_tag="PWY98_002605"
                     /pseudo
     CDS             1725..3227
                     /locus_tag="PWY98_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3972..5798
                     /gene="ltrA"
                     /locus_tag="PWY98_002606"
     CDS             3972..5798
                     /gene="ltrA"
                     /locus_tag="PWY98_002606"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2038312.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5932..>6733
                     /locus_tag="PWY98_002607"
     CDS             5932..>6733
                     /locus_tag="PWY98_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2038313.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2334 a         1107 c         1443 g         1849 t
ORIGIN      
        1 tgaagattta aaaaaacaag tagaaaagct atctcataag agttataaag atgtttatga
       61 tgagtattca aaagagacag atatttatag ggaactgaaa cgtgatgtgg aggcacttaa
      121 ggaaattccc aaaaatctaa ctgatagtgt tgacaaaatt gactttggca atggtataaa
      181 agttacagac gaaattgatg aacaccgcgg agaattgatt aagaatttag tgaccaatcg
      241 ggctaagatt caaacaataa ttaatgaaat gtcagatgaa attgatagtt atagagcaga
      301 gctgaataaa tcagattgga aagttaacta taatgaggtt tcaaagaggt atcaaagagg
      361 tatcaaaaac ttagtgaaga gttatctcaa gatgaattaa aaatgttaca aaaccttgat
      421 tctttatatc aatctttatc aaaaactgaa gagcgattgg aagaaattat tcaacaagaa
      481 ataaagctta aagattacca agttgaggtt gactcagcaa tgtcaagaat gaataaatca
      541 tatcaaaatt tctattctga gagaagtagt tcctcgcgcg aaattttttc tgatattaca
      601 ggaatagagg ctactattca accaaatagg gatttcaata attatgttaa agagctcaga
      661 gcttgtatta ataaggagga cacttttgac agtgagtttg gatctcttat tgataagctg
      721 acaaaaaatt ccattactaa tcaagatata tataatgaca tacttgatat aagagttagt
      781 ggggaaagta gtattttttc agataaaaaa ttaatcaatt ctataagaaa attgaatgat
      841 gcacaagttc ttgaattaca aacccttaag ccaaatgatt tagtagttat ccgacttgaa
      901 ctaaatggaa aaaaagttag tttatcgaat gctagtgctg gtcaaaagac atctgcaatt
      961 ctttctatga ttttggcgta tggaaattca ccactcatat tggatcagcc tgaagatgat
     1021 ttggatagtc aattaattaa taacttaatc gtaagaagta tcattacaaa aaaggaaaac
     1081 agacaaattt taattgtaac tcataatgca aatatcccag ttaatggtga ttcagaatgg
     1141 ttgattagca tgggagattc aaaagaaata tcggctgatc agtgcgggtc ggtagatgaa
     1201 cccaatatca agccaagaat atgtactgtg atggaaggtg gcgaggatgc ttttacaaat
     1261 agagcgaaac gctatggttt taaacaaatt gtagaataac actagagggt gagacatatt
     1321 attatgtccg ccctttttag gaggaaaaat gaaaaaaata aaaagctata ccagtatctg
     1381 ggcagttgaa aaagtcattt acgcaatcaa tgattttcag ttgccttttc ctgtgacttt
     1441 taaccaaatg gcttggttcg ttttatcttt actgtttgtc atcgtatttg cccatgtgcc
     1501 tccactatct atgatagagg aggtcttttt aaaatactta ggcataccgg tggctgtcac
     1561 ttggtttatg tcgcaaaaga cgtttgatgg caagaaacca ctgggatttt tacgatcctt
     1621 tatcagctat cacttgcgat ttaaggtgac ttttgcagga aagaaagtca aagaacagaa
     1681 gaaacgatgg gatgaaccca gtactcttgt aaggagtgtg aactatgtat ccgattaagt
     1741 atattgaaaa taatctcgtc tttaaccaag aaggcgagtg ttttgcctac tatgaattag
     1801 tgccttacaa ttactccttt ttgtcacccg aacagaaatt tcaagtgcat gataactttc
     1861 gccaactaat tgcgcaaaat cgtgaaggca agatccatgc gttacaaatt gccacagaaa
     1921 gtagtattcg cgccacacaa gaacgatcaa aaaaagaaat taccggtaga ctcaaagaag
     1981 tagcgaaaca acgaattgat atacagacag atgcattagt atccatgatt ggcgatagtc
     2041 agattgatta tcgctttttt attggcttta aactaattgc gacagatgaa gaagtcaatc
     2101 taaagagtct caagaaatct ttcttttctg ggcttcaaga atttgtctat ggagtgaatc
     2161 atcatttgat gggcgatttt gtttccttat ccaatgaaga aattcgctgc tattctaagc
     2221 ttgaaaaatt aatggaaagc aaattggctc gaagatttaa agtcaggcga gtcacaccga
     2281 gtgacctgac gtatttgatt gaacatattt atggcgaaaa agggataccg tttgaagaat
     2341 acgaatttca gctaccaaag aaaaagctaa aatcagaaac actggtgaaa cgttatgact
     2401 tattacgtcc aaatcgttgt ttaattgaag aaaaaccaag gtgcttacgg atggaacatg
     2461 agaatcatga gtcgtacgta gcgtatttaa caattaatac aattgtgggg gagatggaat
     2521 ttccttcctc agaacttttc tattaccagc aacaacaatt tacattccca atcgacacaa
     2581 gtatgaatgt agagattgtg acgaataaaa aggctttagc taccgttcgt aataagaaaa
     2641 aagaactgaa agatttagac aatcatgctt accaatctga caatgaaacg aattccaatg
     2701 tcttagatgc tttggattcc gtggatgaat tggaaaccac cttggatcag tcaaaagaat
     2761 ctatgtacaa actgagttat gtggttcgag tgagtgccga atcagtggat gaactaaagc
     2821 gacgctgtga tgaagtgctc gatttttatg atgataccaa tgtaaaacta gtccggccct
     2881 ttggcgatat gatggggcta catgaagaat tcttaccctt aagtaaacga tatatgaacg
     2941 actatattca gtatgttacg tctgattttt tggcggggct tggttttggg gctactcaga
     3001 tgctaggaga attagaaggg atttactttg gctacaatgt ggatacaggt cgtaatgttt
     3061 acctgaaacc ggcattggct tcacaaggag tcaagggttc agtaaccaat gcgctggccg
     3121 ccgctttcct gggttctctt ggtggaggta aatcctttag taacaatctc ttagtctact
     3181 atgctgtttt gttcggtggt caagctgtga ttgtcgatcc aaaagggggt gcgaaacgtt
     3241 cctacttgaa aagagtaggc acagcacttc attaaatcaa tatttaatgt gtgttggaga
     3301 actaaacaca cgaatagtcg aatgacgggg taacgccctg aaaggctact ttaatactct
     3361 gactggcaga aacttgagta aacaaggctc tgtcagaagc tcggtgaagt cggcagacag
     3421 acaccgtaac agataatgct gacgaaagat tcccagaggt gggcgttgtg tcctatatgc
     3481 cggaagtcta taaaatatct atggttagaa tgtagatgag aggtctactg acgaatcctt
     3541 gaatgtacag gtctatatcg gggctggata gaaatgtcca gtgacacaaa cagtgtcgct
     3601 actgtgggtg agtaagactc cgcatttatg aaaatccata taacgttaaa ggcgttatca
     3661 aggtagcagg cttatagagg aaacctaagg atatatgtac agatagtgtg tttggaacgt
     3721 tgaaagctga caacatggag attttactgt ctgtgaagtg ttcacatcag aaagttattg
     3781 tgagattagc aatgatataa ggtgtgttaa tgtcagtgag agtggtggca cagtacctat
     3841 gaaaccgtga taacaagcgg tggagggata gccactagtc atatgaagaa tagaattact
     3901 aagaaaaatg gtatgggttc gagtaagact aagagatgta cttgctcctg caaacaagga
     3961 gggtacagac catgctaaag aaaaacaagc tccgctataa cgagtattat gatatgcaac
     4021 atatttatga cgagttatac gcacagagca agaacggtaa taacttttat aagttacttg
     4081 aaattattgg ttcggaacaa aatatttgtt tagcttaccg taatctgaaa tcaaacagtg
     4141 gcagtaaaac ggctggaacg gatggaatga cgatagacga tataaaacaa ctcagcaatg
     4201 ctgagattgt tgctaccgtt cgggaaagcc ttagcaacta ccgccccaaa tcagtcagac
     4261 gtgttttcat tccaaaagct gggagcgata aaatgcgtcc attggggatt ccgtgtatct
     4321 gggacaggct ggtacaacaa tgtatcctgc aagtcctaga gccgatatgc gaaccaaagt
     4381 tccataatca ttcctatgga ttccgtgcga atcgaagtgc ccaccatgca gtcagcagag
     4441 taacgacact gataaattta tcaaaatatc attactgtgt tgacgtagac attaaaggat
     4501 ttttcgataa tgtcaatcat ggaaaactat taaaacaaat ctggacactt ggtattcggg
     4561 ataaaagatt gatttgcatt atcagcaaaa tgctgaaagc cgaaattgac ggcgagggtg
     4621 tcccagaaaa aggaacgcca caaggcggtc tgttaagtcc actgctctcc ctcatagtcc
     4681 ttaatgaatt agactggtgg gtaagcagtc agtgggaaac attccagccg aagaaccgca
     4741 gtaaaaatgg gtggcttcaa tacgcaaaga aatataccaa actgaaaagc ggttttattg
     4801 tacgttacgc tgatgatttt aaaatcatgt gttcaaccta tggagaagca caaagatttt
     4861 accacagtac cgtagatttt cttaataaaa ggctgaaatt agaaatcagt ccagagaaat
     4921 caaaggtagt aaatctgaag aaaaattcat cagattttct ggggttcaaa atcaaagtca
     4981 ttccaaaagg aagaacgaaa cacggttatg ttgccaaaac agatatgaac caaaaagcat
     5041 taaagaaagc gaaaacaaat ctaaagttaa aggtaaaaga tatagccaga catacgactg
     5101 gcttcaatat cagcagatat aatctgacag ttatcggtat gcagaattac tactgcatag
     5161 caactaacgt atataacaac ctgacagaag taagttacgc tctcttaccg accatacgaa
     5221 tccgtttaag gaacattgca aaatcagttc catttgaaag tacaagttca gaatttcaga
     5281 gcaggacaaa aggaatcaga ccaaaaacaa aaatagtgat gattgcggat aatccgctat
     5341 tgccgattca aggagtacaa cacaaaaatc ctatgaattt ctcgcaagat atttgtaatt
     5401 ttacaaagca agggagaaac aaggtgcatg aggacgttgt agttgtaaca aaagaagaaa
     5461 tccgagcgtt gttggaaaat gaaaaccctg ccgatagcgt agagtttaac gacaatcgta
     5521 tttcggcata tatcgcacaa caaggcaact gctatgtaat gaatcgtcga ggaactccgt
     5581 ccactctgat atgtattcac aaatcagaca aaggagataa ccttgacaga tattctaatc
     5641 tggcatttgt ggaaattccc attgcaaagg caatcctaac agaatcagca gacgaagcaa
     5701 aatcactgtt aaaaggttat gttcttaata gtcagcagaa gaaaaaactg aacagaatac
     5761 ggacgaatta cggataccag acaataactg ggaaataact tataaatcta tttggatatg
     5821 atggaacgcc gtatgaagcg aaagtttcat gtacggtgtg aagcggggga aaacttggag
     5881 ataacatcaa aaagttacct atcgctatag cgaggagggt ggaaagaaac cttgccagaa
     5941 attgcggatg aaatcagtat cctcaacttg acgagtaagt cagaaaatca aggtctgctt
     6001 gatccttatg tgattatgaa gaaaaagaaa gactcggaaa gtttagcaat tgatattctg
     6061 acttttttaa caggaatttc aagtcgagac ggggagaagt ttcctgtgtt acgtaaagcc
     6121 attcgaacgg tgactcaaag tgatcaacgg gggttattac aagttattaa cgaattgcga
     6181 aaagacgggt caccaatcgc ggaaaatatc gccgatcata tcgagtcctt taccgattat
     6241 gattttgctc atttgctttt ctcagacggc tcgattaccc aatcgattag tttagataaa
     6301 cagctaaata tcattcaagt ggcggatttg gttttaccgg atgcagaaac gacttttgaa
     6361 gaatatacca ccatggaatt gcttagtgtt tcgatgctaa tcgtcatttc cacttttgcc
     6421 ttggacttta tccatagtaa ccgagaaatc ttcaaaattg tcgatttgga tgaggcttgg
     6481 agttttcttc aagtggctca aggaaaaacg ttatcgaata agcttgttcg tgctggtcgg
     6541 gccatgaatg ctggggtcta tttcgtcact caaaatagcg acgatttatt agatgaacgt
     6601 ttaaaaaata atatcgggtt gaagtttgct tttcgcagta ccgatattca tgaaatcaag
     6661 aaaaccttgg aattctttgg tttggatcag gaggatcaaa gcaatcaaaa acgcctgcga
     6721 gatttggaaa acg
//
LOCUS       DANKAY010000130         6702 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000130 DANKAY010000000
VERSION     DANKAY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            10..2337
                     /locus_tag="PWY98_002608"
     CDS             10..2337
                     /locus_tag="PWY98_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2038314.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2463..2792
                     /locus_tag="PWY98_002609"
     CDS             2463..2792
                     /locus_tag="PWY98_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2038315.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2832..3836
                     /locus_tag="PWY98_002610"
     CDS             2832..3836
                     /locus_tag="PWY98_002610"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2038316.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3833..4279
                     /locus_tag="PWY98_002611"
     CDS             3833..4279
                     /locus_tag="PWY98_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2038317.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4429..5244
                     /locus_tag="PWY98_002612"
     CDS             4429..5244
                     /locus_tag="PWY98_002612"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2038318.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5450..6622
                     /locus_tag="PWY98_002613"
     CDS             5450..6622
                     /locus_tag="PWY98_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2038319.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         2386 a         1262 c         1332 g         1722 t
ORIGIN      
        1 agaggaggga tgaaaatgat taagaaaact caaaataaaa tccaacgtat atgtcaaaaa
       61 cattttgttt ctagactcaa agctgtatct aaaagaaaat acttacatcc cattatgatt
      121 tcgatcatct tgatttttac tttcttaggg agcttatcaa gtatttatgt aaacgtttat
      181 acagaaacgt caaattttgc taaccaaacg acagaggaga caaatagtcc accctattct
      241 gaaatacagt ccatagaaag caccaatcag tctagcaaca acgagacaac ctcaaccaca
      301 tcctcgactc aactgattga tccgacagaa aaagaaatca cagcagcaaa agcagcaggg
      361 gagaaagctt attcagaaac aggtagaagc cagacattta gtactgttgc ggccactgcg
      421 aatttcaacg gacataccat accagatgga tatacagtga tatatgccaa agatggagtc
      481 attgttgcgg ttaacggatc gatgagtgcg gataaaactg cgttacaagg cgtaaccaaa
      541 gcaatcgctt ttattgataa ccgtccagat atccataatc tgtttattgg ctccacagta
      601 aatggtacta ctatcacatt cccaaaaagt atctatatgg gggtggctac cgctggaaat
      661 acgatcgatt cagttacacc caatgcggat aaaagttatt cttttacacc ctcaacatgg
      721 ggagggcttc ctgccggagg aggtatgagt actttttcaa tcggtataca gttagcaaat
      781 gatattccat tcacggggta ctcgatcaaa aatgggcaga tcgttgatgg taagggaaat
      841 gtttcaaatt ttccaaccta tcaagcagca acggttcagg tcaatgcgaa tcggacagga
      901 actttttctt atacagtcaa tggtggagca gcaaaaatcg gaacgtacgg gactacatta
      961 tctggaatcc atgtgggtga caaagtggtt atcactccaa cagcagtaac gggctattct
     1021 tttgcacaaa caaacggttc tttcacagcc ctagaaagta acgtcgatac ggtgacctat
     1081 acaggcaaac aaacaccaat cactattaag tatcaagact attttggaca gatgattgct
     1141 ccttctaaaa taatgaatct tacttacgga agtgctgcac aagacctgac gactaatacg
     1201 cctactatca acggatatac ctttacagct gtttcagcga tagaaacaaa gaaccaatca
     1261 gcaatgagtg ttttagctag tttagataca aatggaaatg taatcgtaaa ggacgcaaat
     1321 ggaaaacaga tttctgaagt catcctatat tataagacaa atgcaacagt atcaatcaat
     1381 gcaaatggca gtaagtacta tgatggtctt tctgttgtac cgatcgttaa atatgccttt
     1441 aatgataaaa cagaatcaac atccctttta aacaatgatc tcagtattga tcaaatcaca
     1501 tggaatccaa ctgatttcaa agcgatgaat gaaaagggag ttgaagtaac tgcgcctact
     1561 gaaattggaa cctatacgtc ttggcaattg acaaaaagcg gactagctaa actagctgcc
     1621 agaacaaact atttatttac tatcgtgcag acatctgatg tctatacgat taagcaaatc
     1681 agcggtgcgg tagagctcag tggcagtaag acatatgatg gaaaagcagg tgtcccgtca
     1741 atccatgtca agctagcaga gggtgtcact agttcgctta ctccagtaca agtggcactt
     1801 agcagtatgg attacacagc agatgcacaa tcagcaaaaa atatggttaa tgctggcagc
     1861 tatacaatta aactaacgaa tagcggaata gacaaagtca aagcagcaaa tcccaagttg
     1921 tcatttactg atatagccaa cacatcagga acttatacca tctataaagc taacgcagtg
     1981 attactgtcg atgacgctgc ctttaattat gatgcccaaa gtcactcgat ttcggttgga
     2041 aatctccacg ttaccggtgt tgtcttaggc gaatcattgg attataccct aacagttaac
     2101 agccggacag atattggatc acaaatagta acgattagtt taggcgaagg aagtgtaaac
     2161 aataattata cgattatcgc aacagatacc gctaagttga cgatcaatcc gactctcttg
     2221 cttccttcga caggtgggat aggtctactg ccctttgtct ttttaggact gatctttatt
     2281 ttttctggct tcttttattt tatccatcgt aagaaaaaag caggtgaaca gcgatgaaaa
     2341 aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt ttttatccta ctggtctttt
     2401 ttataggttc tccgactgtg tcagctagag aatcacctat tacatttact ttgcataatc
     2461 gaatgttcga aagcgataag atgccaagta tcagtcaaaa cgacggaata aatatcccgg
     2521 ataactttca aggagtaaaa gggttgaacg gcgttacttt cgctatatat gatgtctcgg
     2581 atgaatttta caagctgcgg tcagaaggaa gtagtgtgga agacgcacaa agaaaactag
     2641 cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt gacaaaaaca gtagacgagg
     2701 aagagggcat tgcttctttt agtgcatcgg acaaagatga gcaaggtcgt gacgctgttt
     2761 accgatttac ggaaataaag acttcagatt aagtaaaaga aaaatcagca ccatttgtcg
     2821 ttgtgcttcc tgtgacagat tcatctaatc ataaattgac gaccatccac ttgtatccaa
     2881 agagcgaaca aaaaatacct gctgcactca cattaacaaa aacagtagag aataagcaga
     2941 cagattttgc agatggggat aaagtccctt atctcatcac cgtaacaatt cctgagaaca
     3001 tcaacgagat aggaacctat acgatcaaag atacagctga tcctcagtta tggctagaac
     3061 cagaaacgat cgattttttg attggtggag acaagattca tacatttcac acacaaaaaa
     3121 taaaacatgg ttttgtctta tcagattctg gaaaagatct tagttcgttt gctggaaaaa
     3181 aattaacgat ccgttaccaa atgacattga aagaaaacag tgaaaaaacg acatttgata
     3241 acgatgtacg tttgacgact gacaatcaaa cagtagaaac acatcttgcc atccaaacag
     3301 gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca aaaactagca gatgctcaat
     3361 ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa cgggataaat cattgggtag
     3421 ccaaagatga ttcaagagca acgattttta cttccagtaa agatggaaca ttttctgtcc
     3481 atggattgtc ttttggaagt tacgaactag tggagattaa accgcctaaa ggatatatat
     3541 tgagtcaatc aaccattctc tttgaagttg aaaaaggcag ttatgatccg cttcatccta
     3601 taccattgga aatcatcaac gaaccaaaag caaatccgac gaataatgga aaaacgccag
     3661 ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg aggaagcttt ccaaaaacaa
     3721 gcgaagagat gcttggaggt ttctcaattt tagggttatt gctagtcctt tcaacaggga
     3781 cagcttggtt ttataaaaaa caacaaaaag gaaacaatag gagggaaata aaatgaaaaa
     3841 catcaaaaca aaaatatgga cgtttttagg aactgctatc atgctgcttc ctttcgttct
     3901 cggcctagga actgcagagg tttctgcagc cgtatcacct acaccagaaa atgttacggt
     3961 taatttgcat aaattgaaat tcacttcagc accagaaaat caaataaaca acggaacgga
     4021 attgacattt ccgaatagcg aaccgttgaa cggcgttgaa ttcaacgtat atgacatcac
     4081 tgctacgtac tatccaagta aagacacagc agttccagca gacgctacac ccttcgcaag
     4141 tgtaacgact tctggtgaag gattagctaa cctgactctt ccaggtaaat ctgatgggaa
     4201 agatgctgta tacgtctttg tagaaacacc aaagcccggt gtcgaaacgt cgccaaatat
     4261 cgtgttaagc ttaccctaga ccctgacaaa cctttagaca cgattcacct atatgctaaa
     4321 aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg ggaacttttc aggggtagat
     4381 aagttagcag gggcgcaatt ccagatcaaa aattcagaga atcaattcgt gaccggtttt
     4441 gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt cagcgttgac attcacaact
     4501 gatactgaag gatccatcag tgtcaatgga ttaatagacg gcaattatac tttagttgaa
     4561 acaaaagcac cagatggtta tcaattaacc aatccacaaa aagaaactac ttttatagta
     4621 aaaaacggaa aattgcaaca aacaaccggt atcatccatg gcgcagatga agccaatctt
     4681 gggaatccaa acggtgtagt caatcgggaa aatacaacta cggataacac gattactgta
     4741 gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac aagatgtgaa cacgcaagaa
     4801 aaattagccg atgcagaatt ttctattcta agagcaaaag atccaactgt taaattaatg
     4861 aaaaaaatag caaaccttgc ctcaggagaa tacccatatg cttggtcaga tgaagtcaca
     4921 ggttctgact gggaagaagt gaaattgatt tctgattcag aagggaaatt ttcaatcgaa
     4981 gggctgaaac aaggggcata ctgccttcaa gaaacaaaag caccagaagg ctacgcatta
     5041 cctaagaatg aagctgcgta cactgaattt acagtagatg atgatccaac aacttctgat
     5101 gaaacaacga tcgataacca acctaaagga atcttgcctt caactggtgg tatggggatc
     5161 atcgtattca tcttggtagg tactgcttta gttggaggag cagtgatcta tttcaaaaaa
     5221 cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa atcatacgat aaataaacga
     5281 tagtgattca aatggggaga aaggttgtga caagtggtcg gtctcttggt attcgctcaa
     5341 caaacggcaa aataggagga actattttgc cgtttgcttc gctaatactc aggtcaaggc
     5401 gctgatttca caacctcttc atatacatat tagaaaatag gtggacatga tgaataaaag
     5461 cagaataaga atactggaca tcttcatggc aatcatttta gtcgttggga tcgggatctt
     5521 ttcttatcct tttgtagaag attcactgaa tgattttctt gcccagcaaa tgatcatcca
     5581 ttatcaaaag caagcatcga ataagaattc agaggaaata aaaaaacagc aggaaaaaat
     5641 gacaaagaaa aaccaacagt tggcagagca aaacgtatca ccagggatag gcagtttcaa
     5701 ccaagcagtt gatgcaaaag cattaaaaga tttacctagt aatgcctttt ttatggaaca
     5761 tatgcttgga gtcatcgaaa tcccaaaaat caatgtcagc ttacctatat tcgatcagac
     5821 aacggaaata tttttgcaaa aaggaacgtc attattggaa gaaagttctt atccgactgg
     5881 cgggaaaaac acccatgccg ttctttctgg gcatcgtgga ctcccagaag caaaattatt
     5941 tacagatttg cctaagttga aaaaaggaga ccagtttttc atccagatca atggaaagac
     6001 attggcgtat caagtagaaa aaatccaagt ggtcttacct gatgaagtag attctttggg
     6061 tatccaaaaa ggcagagatc tcgtgacttt gcttacctgt actccttata tggtcaatac
     6121 acatcgactg cttgtgacgg ggcatcgtat tccttatcaa gcaaaagagg caaaaaaagc
     6181 gatccaagga attgatcaat ggaaaaaatg gaaatttttt atttggttta tcggtatact
     6241 acttggatca atcggcttag tttggctact catcgcttat ttagattctt tggccatcgc
     6301 taagcgaaac tatcctttat ctttttatgt aaagaacaca aacggacgac caatcgaagg
     6361 aatggtcttt tctgtaaaaa cgctcaacgg caaacactat attactagag aaaaagttcc
     6421 atttgtgaaa gcttctgatg aatacggact agtgagattt tctgatttaa aaggcaggaa
     6481 ctatcgcttg caacatgagg agctcctgct taaaatccat gtaaaacata aacattcgaa
     6541 acagttttca atgaaattga aaaaaggaag atataaactg cgaaaagaaa aagaggtgta
     6601 ctatttgatt gagaaagaat gaatgaagat gtccaaaaaa gcagagggaa cagactaaat
     6661 gaaaaaagag agaagctgac gaaatggacg tgtaaactgt ta
//
LOCUS       DANKAY010000131         6668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000131 DANKAY010000000
VERSION     DANKAY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(77..535)
                     /locus_tag="PWY98_002614"
     CDS             complement(77..535)
                     /locus_tag="PWY98_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2038320.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(548..1879)
                     /locus_tag="PWY98_002615"
     CDS             complement(548..1879)
                     /locus_tag="PWY98_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2038321.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1890..3332)
                     /locus_tag="PWY98_002616"
     CDS             complement(1890..3332)
                     /locus_tag="PWY98_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2038322.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3319..4083)
                     /locus_tag="PWY98_002617"
     CDS             complement(3319..4083)
                     /locus_tag="PWY98_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2038323.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4187..5062)
                     /locus_tag="PWY98_002618"
     CDS             complement(4187..5062)
                     /locus_tag="PWY98_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2038324.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5317..5401)
                     /locus_tag="PWY98_002619"
     tRNA            complement(5317..5401)
                     /locus_tag="PWY98_002619"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5365..5367),aa:Leu,seq:caa)
     gene            complement(5419..5490)
                     /locus_tag="PWY98_002620"
     tRNA            complement(5419..5490)
                     /locus_tag="PWY98_002620"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5456..5458),aa:Gln,seq:ttg)
     gene            complement(5500..5572)
                     /locus_tag="PWY98_002621"
     tRNA            complement(5500..5572)
                     /locus_tag="PWY98_002621"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:His,seq:gtg)
     gene            complement(5634..5718)
                     /locus_tag="PWY98_002622"
     tRNA            complement(5634..5718)
                     /locus_tag="PWY98_002622"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5682..5684),aa:Leu,seq:caa)
     gene            complement(5739..5809)
                     /locus_tag="PWY98_002623"
     tRNA            complement(5739..5809)
                     /locus_tag="PWY98_002623"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5775..5777),aa:Cys,seq:gca)
     gene            complement(5855..5926)
                     /locus_tag="PWY98_002624"
     tRNA            complement(5855..5926)
                     /locus_tag="PWY98_002624"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5892..5894),aa:Gln,seq:ttg)
     gene            complement(5938..6010)
                     /locus_tag="PWY98_002625"
     tRNA            complement(5938..6010)
                     /locus_tag="PWY98_002625"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5975..5977),aa:His,seq:gtg)
     gene            complement(6013..6086)
                     /locus_tag="PWY98_002626"
     tRNA            complement(6013..6086)
                     /locus_tag="PWY98_002626"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6052..6054),aa:Trp,seq:cca)
     gene            complement(6093..6173)
                     /locus_tag="PWY98_002627"
     tRNA            complement(6093..6173)
                     /locus_tag="PWY98_002627"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6137..6139),aa:Tyr,seq:gta)
     gene            complement(6175..6250)
                     /locus_tag="PWY98_002628"
     tRNA            complement(6175..6250)
                     /locus_tag="PWY98_002628"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6215..6217),aa:Phe,seq:gaa)
     gene            complement(6286..6358)
                     /locus_tag="PWY98_002629"
     tRNA            complement(6286..6358)
                     /locus_tag="PWY98_002629"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6323..6325),aa:Asp,seq:gtc)
     gene            complement(6368..6440)
                     /locus_tag="PWY98_002630"
     tRNA            complement(6368..6440)
                     /locus_tag="PWY98_002630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6405..6407),aa:Val,seq:tac)
     gene            complement(6456..6527)
                     /locus_tag="PWY98_002631"
     tRNA            complement(6456..6527)
                     /locus_tag="PWY98_002631"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6492..6494),aa:Glu,seq:ttc)
     gene            complement(6542..6631)
                     /locus_tag="PWY98_002632"
     tRNA            complement(6542..6631)
                     /locus_tag="PWY98_002632"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6595..6597),aa:Ser,seq:gga)
BASE COUNT         2006 a         1472 c         1083 g         2107 t
ORIGIN      
        1 ttgaaacata tcatttcaaa aaaattttgt aaattcaccc ataaatatca ccctatttaa
       61 cttttttcat cgtacactaa aattcaaaac aactatcttt aatctttact tcttggtttt
      121 gttttaataa atttccttca aaaatgcctg gagaaactac aatgatcacc gtatcttttt
      181 gtgcttgctc caacttcttc aaatctataa tagctaacag tgtcccttct tttacctcct
      241 ggttactttc aacccgtatc tgaaacggac ttccttcgag ttcaactgta tctaaaccca
      301 tatggatcag tatttttctg ccagattcac tttcgatcgt tatggcatgt cgggtaggaa
      361 atatattatc gattattcct ttaactggcg catggatata tccatcatga ttcgttatag
      421 cgtatccaac tcctatcatt tttgaagaaa agacctcgtc ttttactgct gttaatggaa
      481 taatttgacc actagctaca gcttttagaa tgtttcttgt tttaaagaaa tgcacttttt
      541 atcacctcta ctttaatgct ggccaatatt ttttgtttac tttgatcatt tcatctagta
      601 ttttttttgc gactctggca tttggtacgg tttgattcaa tgtaaatgct tgtaaagctt
      661 tttgataaga accttcaaag taagcatcta ctagtaactt ctctactgca acttgtgcct
      721 ccatcaatcc tttatgaaaa tcactgattg gttttcttag tgtaattggc tccacaccag
      781 ttgcaccaac atatgccggt atttcaacaa ctgcatctga ccgtagattc ggaatcgccc
      841 cttgattagg aacgattagc ataaagcgac gacgatcatc attcaacaag gaaatagcta
      901 tatcgacgat atattgtcca tgctccccaa aattaaagtt caatacttct tccacaatac
      961 cgcggcgtat tttatcagcc atttctttgg tatttttctc tcgtccatcc atgaccatat
     1021 tagcacgtgt atactctttg tcagtatgct caacgaccat atcaggatat aagtaatact
     1081 ctaagtaatt attcgggata ttacttggaa aatttttcgt gatgactgac atcatttgaa
     1141 aggttttttg ccaagtttta tctcctatat taaaatcggc gacttgcatt tcttttgtta
     1201 ttagtttttc aataatctct ggcataattt ctcgttgcaa ttctttatca taaatcgatg
     1261 tataccagcc aaagtggttt aaaccataat acgtaacgat ccaattctta cgatcataac
     1321 cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat atcacaggca ttaatcattt
     1381 ttacattcgg atatttacga cgaacagctt cagaaacgat cgactctgga tttgtataat
     1441 tcaaaatcca tgctttgggg gcatagtctt gtatatgatc gacaatttcc aataaacctt
     1501 tgattgagcg catcccataa gcaaatcctc cgagtccaca cgtttcttgt cctactaatc
     1561 catgtttaag tggaatcttt tcgtcctttt ctcgcatctc caatcctcca acacgaattt
     1621 gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg atcatccgtt gcacgaatgc
     1681 gaatatttga aaaacctttt cttttcagca taaatttaac aatcaagtac atatccatat
     1741 ttctttcttc atcaatatca tataaacgta tttcagataa tggcaaccgt ttttcattgt
     1801 tcaaaatggc ttgaacgatt cctggtgtat acgtactccc gcctccagct atagtgataa
     1861 tctgtttttt catcatcatc ttttcctttc tattcttcta ataatttatt gaattctgcc
     1921 ataacataag tcacttctaa gccgatgata atttgataat ttttttcagt tggtttaatg
     1981 atcccgctta tccctaattg cttgattcgc tgttcctcga ccatggtggg atccactacc
     2041 tctacccgta ggcgtgtatt acaactatag acatccagga tattggcttt tcctccaaac
     2101 gcttgtagca tttttgttgc catatattca taatttttac ttgctaaccc taactctgtt
     2161 tcttctaagg ttacttcatt gccaaactct gccttagctt cacgtcctaa tgtctgaatg
     2221 tttctcttat tgatcaaata ataaaaagta acgtaataca aagcaaaaaa gaaaattccg
     2281 attggaagaa tcaaaatagc attggtagcg attcgaaaat tgatgagata atcaacaata
     2341 catgctccga atgtaaaacc aattcgtata ttgaataagt aacagacaat ccctgctaaa
     2401 ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa gaaacatgaa actaaattca
     2461 acaggctcag tgatatttgc aataaatgaa gttcctgcac cgcttcccat gattcctttg
     2521 accatctctt tattttcttt ataagctgct ttatagatag ctagacatat agctggtata
     2581 ccaaacatca ttactacaaa aaaaactgat aaaaacagtc cagcctgagg atctccgttg
     2641 ataaagcgtg tcatctctcc tctgaaaact tccttgctag gagatgtata gcttcctaaa
     2701 tcgtaataaa tatacgtatt caacacatgg tgcagtccaa aggggatcag caaacgattc
     2761 aaaaacataa atatccccac acctaatgct cccatatcaa cgagcagttt tgcaaaatta
     2821 tcaatcccaa tttggatgag cggccagata aagcccaaaa aaacagctgc gattgtttgt
     2881 agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa aataagaaaa aactactggt
     2941 aatttttgtt ctttaaagcg attatacgtc catgccgcta ctaaaccgga aataattcca
     3001 ccaaaaatcc ccatattcac ttctgggtcc ataatttcta agcctttttt caaggtagca
     3061 atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct tatttttgac aaaaccgatt
     3121 gcaactccta tagcaaataa gatatccaag tttccaaaaa cggcatttcc agctgcacca
     3181 attactggga tgttcagcat atcgtcagct gataaacgca tgatcaatcc cgctaatggc
     3241 atcgctgcaa ctggaagtag catcgctctt ccaagcttct gcacatttga tttaacattc
     3301 atttttattc ctcctctatt aaatatcctc atatgttact aactcaatca aaggatcatt
     3361 tttcactttt tgagcaaatg ctgaatgaat taaaaattcg acttggtggg tacgaggata
     3421 cacaaaactt gaaccattta acaattcctg atccaaatac gctgcgtgac acatgacttc
     3481 gatcgtttta tcttctctca atatcatgag taaacttgcc aaatattctt ccacactcgt
     3541 attgatttgt cgtttttttt catcagtttc tcctacgaca tcgaaacaag gctcaaaata
     3601 atcgacatga cgaacttcat cttttcgttc aaagttgctc cgaaccggca aatcatattt
     3661 ttttgctaat gtaacaacta cctcctgatt ttcatccaaa gtatgagtat gatgatgact
     3721 gtctaaatga gttggtatga tccccgcatg ataaattttt tgaatctgag cattccattc
     3781 ctgatacagt tgatctttgt caatgttaaa tggatgctga taataagata aggatctgaa
     3841 ctctccgctt tcgataagag tatcgacagt tttgaggagt ggtttgccac acgtcaatgt
     3901 taaatggact cctacaccta gcctaggcat ttcctttctt agtttcacag catgctcgaa
     3961 acccggcata tttgccatca atgtagttga tgtcaaaatt cctcgctgat aagcatccat
     4021 aatcccataa ttcacaccat ggctatagcc aaaatcatcc gaattaaaaa tcacttttcc
     4081 catttcattc tccttctttt ttctactaaa ataggcgcaa ccaaataatc atagtccatt
     4141 tactatgtgt catttggttg cgcctataga tagttacgcc cttattttaa tagattcttg
     4201 ctagatgaat tgccaaatag atgatttcat tttcttgaat ggcaagttga aattgctgtt
     4261 cgatgatatc tttaatcttt agtgctgtac aatacgcttt gctatgttta aatgtttcag
     4321 taaaaaaacc aatattttct actggcgttt cttcctgata aatcaagcgt ttaaccagga
     4381 atttgatatg aaccattaag cgatgataag tcagtgattc tttatcataa acgtttgaat
     4441 aggatttttc tagatgggac aaaatcgtgg tcgtcatttc tactattttt aagctcttta
     4501 aactattagg ctctcctagc tcagcattaa tgatatgcat agtgatgaat cctacttcat
     4561 ttgaccctaa ctcggtttga tatcgatgat tgattcgctc gaccacttct actgctatat
     4621 catattcatt tggataaagg aatctcattt ctggcaacat aattgctgtg acatctctat
     4681 cttctctttt tcgttgaata gcaaaataaa tatgatctgt caatgtaata tagatatttg
     4741 aatctaatgg agaggagatc ctagctgtta tcagttcaat cacttcttcc gcaaaatcca
     4801 taaattcgat tggaatctgc gagactatct cactgaatcg tttgatcttt tctttagaat
     4861 ctaatacaaa taatttgtca gcatcctcaa catcaaatgg cttactcact ttagcagaaa
     4921 agccaatccc tttccccatc gcaatttttt ctattccatc gtcatcgatc aatacagcat
     4981 tatgattcag ctttttcatc acatacaccg ttaattcacc tcctctatat gataaaacaa
     5041 aacaacttgc agactccttc atacatattt ttgtctccaa acaattttta aagacaaaaa
     5101 acgacctaag aaagaaccct ttcttaggtc gtgcctaatt gaatagtagc accctacata
     5161 atttacaaaa atagtttaac acataataaa agcgttatca atatcttttt tactcgtttt
     5221 atgataatac ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa aaaaatatag
     5281 caactctcta taagaaagtt gctatattga taattatacc ggcggccggg gtcgaaccgg
     5341 cacgtcctca acggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg
     5401 caaatttaaa aataataact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag
     5461 tcccttgcct taccgcttgg ctatacccca agaataaaag gcgaatgatg ggaatcgaac
     5521 ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc gccatcataa
     5581 aagatgttat ttaatttctc aataaaaaac taagatgaag acatcttagt caataccggc
     5641 ggccggggtc gaaccggcac gccctcaacg ggcactggat tttgagtcca gcgcgtctgc
     5701 caattccgcc acgccggcaa gttgatttat tagtactaag gcggtaaccg gatttgaacc
     5761 ggtgatgaag gttttgcaga cctctgcctt accacttggc tataccgcca ttatactatt
     5821 atcaatctat aaaataaaga ataaattata gaaactgggg tagctggatt cgaaccaacg
     5881 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaaaagggc
     5941 gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg
     6001 ccacattcgc catggcaggg gcagcaggaa ttgaacccac actaacggtt ttggagaccg
     6061 ctgttctacc tttaaactat gcccctataa aatggaggag agtggattcg aaccactgaa
     6121 ccctaaggaa cggatttaca gtccgtcgcg tttagccact tcgctactcc tccatggtgg
     6181 cgcgggacag aatcgaactg ccgacacacg gagcttcaat ccgttgctct accaactgag
     6241 ctaccgcgcc atattttaaa attattaatt gaataacaag aacgacggtt ccgacgggaa
     6301 tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa cccctacacc acggaaccag
     6361 ttcattatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
     6421 cccagctgag ctaaacctcc aacattcaat taaaatgacc cgtacgggat tcgaacccgt
     6481 gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca acgggccagg taaatttgct
     6541 acggagagta agggattcga acccttgaga cagtttggca ccatctacat gatttccaat
     6601 catgctcctt cggcctctcg gacaactctc cagagtgaaa gaagctcaat actctttctc
     6661 aaaatgag
//
LOCUS       DANKAY010000132         6626 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000132 DANKAY010000000
VERSION     DANKAY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            494..1084
                     /locus_tag="PWY98_002633"
     CDS             494..1084
                     /locus_tag="PWY98_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HDL2038325.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1111..2421
                     /locus_tag="PWY98_002634"
     CDS             1111..2421
                     /locus_tag="PWY98_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2038326.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2418..3137
                     /locus_tag="PWY98_002635"
     CDS             2418..3137
                     /locus_tag="PWY98_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2038327.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3152..4237
                     /locus_tag="PWY98_002636"
     CDS             3152..4237
                     /locus_tag="PWY98_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2038328.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4373..5905
                     /locus_tag="PWY98_002637"
     CDS             4373..5905
                     /locus_tag="PWY98_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2038329.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            6083..6304
                     /locus_tag="PWY98_002638"
     CDS             6083..6304
                     /locus_tag="PWY98_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HDL2038330.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2320 a          719 c         1273 g         2314 t
ORIGIN      
        1 cctatttgtc taagatactt atgaacgatg agttgattgc ctgtattcac tcatacccat
       61 ataaacaaaa tccaattaaa caacgaatat ttaactattt tttactaaac caatccatta
      121 ttggggttaa aacattagca tatttaagcg gaatgggtag aaataggaga ttttaatcat
      181 aaaaaactgt actatagtga atttttggag atttatattc cagttatata ttaatgattg
      241 tgatacttgt actattgtaa aaattcgtta ggtttaaata actggaaata tcggaaaatg
      301 ggcattatac atcacggaca tcccttcttt tggaaaaaat gttacgattt ttataagtaa
      361 tattgtatcc ggtggaatca acgatgaaga taacatatcc atcgttgtag attcagtagc
      421 attgaatgat gctcttgttg caagaaatcc gaataaaatt gttaagtata aattgaatta
      481 ataggagata tgaatggaaa ttagcagtaa agaatattta aaagcggttc ttcgtatgga
      541 aaagaatcta tatatagaat ctaaaggaca acacacaaaa ttactaaaaa taatgaaatc
      601 acatccggac tacaacgctt ggcggtatac tcgtcgaatg agaattacat cttattatta
      661 ttcaaagaga aagaaaaata tcatttatgc gattatgtat attgtgtctt gcagaagaat
      721 gaatagtttg ggaagaaaat gtggtataga gtgtggtgaa aatgtatttg atgaaggtgt
      781 aagattttac catacacaag gaattgttat taatggtaac gccataatag gaaaaaattg
      841 ttacttatat gggaataact gtattggaaa tgatggaatc gatcctaagt gtcctgtaat
      901 tggaaataat gtacgtgtat gcgtgggtgc aaagattatt ggtaatgtaa aaatagctaa
      961 caatgtagtt gttgcggcag gagctattgt tatcaaagat tgtctagaga atggtgcaat
     1021 tcttgcaggt gtaccggcaa aaataatagg gtatgttaat ttaacaaaag tatttttaga
     1081 ataattttta gcgaaagaag gtgaataacg atgcgtattt caataaaaag atggtcagga
     1141 gcagattgga cattgttcac gttggcattt tttgcaatct ccatagttct tgtatatact
     1201 tttaacgccc caagagcaat tttatatttg ggagatataa ttaatatctt tgtgttatta
     1261 aatgcactta gctttagacg cagaaaagga atatatgcta gcaaaaatga gatgctgata
     1321 gttttgtttt tactagtttc tatttgtagc tcagttatta atttagaaaa tataggattg
     1381 attatctggg ggatacgaaa taacactcgt atgttcatag tatatataaa ttgtgcaaca
     1441 tatttaacaa ttaaagatta ctataaagta ttcgctataa ttgaaaaatt attctggatt
     1501 tcactgccac tttgtttaat tgaaagattc atggtcacct atccaactgg aacaattgta
     1561 ggagatatga taggtggagt tttttggaat tattcaggtt gtaatatgcc attgaattta
     1621 attttgtgtg tgacagttac tgttgttaca atcggatatt tcaagggaga aaaaaatttc
     1681 atccatttta ttttatgtgt ttgtgcagca ctttttatgt ctgcattagc tgaattgaaa
     1741 gtttttattc ctgaattatt tataatattt gttgcaatag tattcacttt aaaagttgat
     1801 tttaaagtag tgatcaagat attatcatgc gtaatacttg tgtcgatagt attatccttt
     1861 tttataacaa tatttattac attgaacgat aatggacaaa gctatgctga taatttcaca
     1921 attcaaggtc ttttagaata tgcgtcaaga gattctggat atgatggtgt tggtgattta
     1981 aatcgaataa ctggtatctc tagagtagta gatgaaatat ttaataataa ttttagatta
     2041 gtcctaatgg gagtaggcat tggcaatgca gagtttacca atttttttgt cagtgagttt
     2101 tataaatcgt ttggtaatct tcattatcag tattttcatc atatttggtt atttatagaa
     2161 gttggaatat taggagtttc gatattccta ttttttataa taaagacatt tttcaatgca
     2221 aaaagatctt ttatgaagga taaagatgca agtcttgtta aagttattgc aattatgtcg
     2281 gtattctttt ttgtgtataa cacaacactt aggggtgaaa caacggggcc actgatttat
     2341 atgattttag ccatgcctga agttgtgaaa aggaaagagc attattatag tgaaaaaaat
     2401 acaaaaggag agatattatg attcctaaga agatacatta ctgttggttc gggggaaatc
     2461 cacttccgaa aaaagataga aagtgcatag aaagctggaa aaagtattgc cctgattatg
     2521 agattattga atggaatgaa tcaaattatg atttaaataa gaaccgttat atggccgatg
     2581 catataaaga acgtaaatgg ggttttgttc cagattatgc aagatttgat attatttata
     2641 atcatggtgg attttattta gatacagatg ttgagctaat taagtcgtta gatatacttc
     2701 gtgaaaatca tgcctatatg ggatttgaag gggaaatctg ggttaatggc ggtattggtt
     2761 ttggtgcaga agaaggacat tctgttatta aaggtttaag ggatatttac gattcattaa
     2821 gtttttatca aaatgatggt acattgaact tgaaaccgag tccgtattat attacagatt
     2881 atcttgaatc tattggattg gttcgcgata acagcgaaca aataataaat ggcctgcata
     2941 tctatcctac ggattatttt gctgccaagg attatgatac tggaaaaatt tataaaacta
     3001 gtaatacaat aagcattcat caatataatg catcttggaa aacacctaaa caaaaatttg
     3061 tttatagatt aaaatcaaca attggacaaa aaaaatttaa caggttactt gcactgaaaa
     3121 aatttttaca cttataaagg agaaaagtac tttgaagaaa atttgtttca ttagtggtgt
     3181 aatttctcgt tctgggggaa ctgaaagagt agggagtatt attgccaata gcttgtctga
     3241 tttaggttat gatgtcacta ttttaagctt ttggaatagt ggagagcctt attttgggtt
     3301 agataagacg attaaggttg attatatgtt aaatcctaaa acggagggaa aattatatag
     3361 aacttattta tatccggttt ggaaattgca caagtatata tgcaaaaatg aatttgatat
     3421 agtaattgat attgatactt tgttgtcttt gtatactaca agggcaatta aaggaacaaa
     3481 gtgcaaattg atttcttggg aacattttaa ttactggaca atggaaatgc tgaaagaaaa
     3541 gaagagattc aaggctaaaa aattgataaa aaaatatgca aagaggcttg tggttctcac
     3601 agaagaagat cgtcaaaaac atattgatga atatagttta tcagaagatt ttgttataac
     3661 tatgccaaat ccgtgtatat ctaatatcga gtacgaatat cattatgata aaaagagatt
     3721 tcttgcagta gggcgattag tggaagagaa aaattttgct gacatgataa gtgcttggaa
     3781 aattgtagaa aaaaaagtag atgattggga tcttatcatt gtaggaaaag gtgaattaga
     3841 aaaagatctt cgccagcaga taaaagattt aaatctgcaa agagtaacta tagcagggca
     3901 ttcagaccat gtggaggaat attataaatc tgcatcttgt tatgtgctta gttctgttta
     3961 tgaaggattt cctatggtta tattagaagc acagtcttat ggattaccag ttatttctta
     4021 tgattgtaaa actggtccga gagatttggt acatcataat tttaatggga tgttggttga
     4081 agataagaat atagaccaat tagctaagag catgataatg tttactaaga atactgattt
     4141 agcaatgaga atgtcactga acgcatataa taatgtacaa aaatttaatt tgaaagaaat
     4201 tacaaaacaa tgggttgctt tgattgaaaa tgtatagttt tttgtggttg tccaaacatg
     4261 aaaattagcg taattaatca aagagctaga agaaacctat tacatttcaa gattttagaa
     4321 attatatagt attaggtaac aataaaaaat agaaagtttg gagccatatg atatgataat
     4381 taatcgtaaa caaaatgcta aaaggaatat aattactgga acaattttta agttatatca
     4441 gattatcatt ccctttttta tgcgtacact tatgatttat cagatgggaa tggagtatgc
     4501 tggattgaac aacctgttca catctatatt tcaaatgtta aatttagcgg aattgggaat
     4561 aggtgctgca ctaactgtaa gtatgtataa acctatagca gaagacaata gaaaagagat
     4621 ttgtgcttta ctgaacgcat ataaaaagta cttttcaatt attggatgga tagtgtttac
     4681 attaggattg ttatgcattc cttttttgag atttttagtt aaaggttcga ttcctggaaa
     4741 tttaaatatg acagttttgt atttaatgta tttgataaat actgttttgt cgtattggtt
     4801 attttcatat agaaagagtc taatatatgc acatcaaaga aatgatgtaa ttaacgtgtc
     4861 tatgctaatt acttttacat tccaatttgt aatgcaggca tatgttctga ttttcttaaa
     4921 aaattactat ttgtatttat tggcttctat tttgggacaa atagttctta acttaatgtg
     4981 tgctgttgtt tctcaaaaga tgtatcctga ttatactcct gttggtgagt tagagaaaga
     5041 aactattgac gatattataa aaaaagtaaa aggattagtt acaaacaagg taggcggtac
     5101 aatattacga tcttcagatt caattattat ttcatctttc ttaggtcttt cagtacttgc
     5161 gatttatcag aattattact ttattatttc tgccataatg agtatatttg caataattta
     5221 tgaatcatgt atagcaggta taggtaacag tcttattgta gaaagtcaag aaaaaaatta
     5281 tatagatttt tgtgatatta caacaattat aggttggttt ctggcaatta gttgtgcatg
     5341 ctttattaca ttatttcagc cgttcatgat tatctgggtg ggaagtgata atcttatgag
     5401 tatttcattt gttatattta tgatagttca ttttttctta tatgaaattg atcagttaat
     5461 tgggttatac aaagatgctg ctggaatatg gtacgcagac aggtttagac cattaattac
     5521 agcaatgata aacattgtat taaatatcat cctcgttaat tggttcggat tatatggagt
     5581 tttggcggca accattgttt cagtattgtt ggttgattta ccctggttat tatataatgt
     5641 tttccaaaat atatttaaat ttgaatcggc aggtaaatat gtttggaagc tcataagttt
     5701 gtgtgtaata gcttttgttg cttcttattt aagttatttt gcttgttcgt atgttaatat
     5761 cggaaatttc aaagtgataa gtttagtgat aaatttagtt atatcggttg tcatatcgat
     5821 tttagttatt cttctgttta atatccgtaa taagaagttt aagtatattt tgaaagttct
     5881 tacgtcggta ttggtaaaaa gatgagaaga gacatcagaa tgatatataa agtaactaca
     5941 atttttaggc tgccgaatag gcgaacgatg tatagttttt gttccaacga agactcttgt
     6001 tgaaattgga gatgatatga aggagttaca attttgactc atgtttatga ttgatcggtt
     6061 ttaaaagtag tatggagaag ctttgggatc atcggagagg atcaaaatag gtaataatgt
     6121 ttttatagga atgaagtcta gaatactaaa gggtgtacat ataggaaata atgtagttat
     6181 aggtgcaaat agtttagtaa acaaggacgt atcagacgat tgtgttgttg caggaaatct
     6241 agcaaaagta attatgtccc tagatgaata ttataaaaag ataaacaata tgaagaatcc
     6301 gtaaaacttg tgaaactata ccgagaataa tatgggaaaa atattgatga aaaagcatta
     6361 tatgagtttt tagggctgtt tacagatgga gatgacaatt tgtcgaagag ttgggagcaa
     6421 tgcaaaattt agtaggggat tataaacaga ctgacaaggt atttaaagaa catgcacaag
     6481 tatttcataa tattgaagaa tttttgaata ctgtgagata ggagttgcta cattgagaaa
     6541 agtatgtatt tttccaatgt aaaagattac gagtttgaaa acgacaccat aatattattt
     6601 tttaagaaga tattatagag cttagg
//
LOCUS       DANKAY010000133         6612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000133 DANKAY010000000
VERSION     DANKAY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(324..893)
                     /locus_tag="PWY98_002639"
     CDS             complement(324..893)
                     /locus_tag="PWY98_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2038331.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="PWY98_002640"
     CDS             complement(967..2256)
                     /locus_tag="PWY98_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038332.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="PWY98_002641"
     CDS             2610..3458
                     /locus_tag="PWY98_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2038333.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="PWY98_002642"
     CDS             3472..4545
                     /locus_tag="PWY98_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2038334.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..>6612
                     /locus_tag="PWY98_002643"
     CDS             4535..>6612
                     /locus_tag="PWY98_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDL2038335.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPV"
BASE COUNT         1972 a         1315 c         1199 g         2126 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aa
//
LOCUS       DANKAY010000134         6443 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000134 DANKAY010000000
VERSION     DANKAY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(80..1210)
                     /locus_tag="PWY98_002644"
     CDS             complement(80..1210)
                     /locus_tag="PWY98_002644"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2038336.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1270..1497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1859..3061)
                     /locus_tag="PWY98_002645"
     CDS             complement(1859..3061)
                     /locus_tag="PWY98_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2038337.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3121..3324)
                     /locus_tag="PWY98_002646"
     CDS             complement(3121..3324)
                     /locus_tag="PWY98_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2038338.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3466..4698)
                     /locus_tag="PWY98_002647"
                     /pseudo
     CDS             complement(3466..4698)
                     /locus_tag="PWY98_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4861..5673
                     /locus_tag="PWY98_002648"
     CDS             4861..5673
                     /locus_tag="PWY98_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038339.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1934 a         1316 c         1008 g         2185 t
ORIGIN      
        1 taaataaaaa agagtggatt tctccactcc atgaaaagcc gactttttgg gacgcttttt
       61 tttgccaagc ctcacatttt tatagccaaa cttcttttgc gacttgctgc aataattgga
      121 ttttcttcca ttgttcttct tctgttaaat ggtttccttc ttctgttgaa gcaaacccac
      181 attgaggact gatacttaga ttttccaatg gtacaaattg ttgtgcttct tttattcgtt
      241 cttttatttt ctccgtatct tccaaatctg gaaacttaga agtaactaat cctaaaacaa
      301 ctttactttc tttgttactc caatgtctta aaggagtaaa atctcctgct cggtcagaat
      361 cgtattccaa aaagaaacca tcataatttg ttgaaaacaa ctcttctgca atgggttcat
      421 atccaccgga aaacagccaa gaagaagcgt gattcccgcg gcagatatgt gttgtgatcg
      481 tcaaatcttc tggtttgtct tccagtgcgt tattgataat ctctgtacaa atatgggcca
      541 tttctttcgg atcaattcct ttttgaagaa gtgcttctct tttagtttga tcagctagaa
      601 acgcccaatt tgtatcatcg aattgcaggt aacggcaacc tttttcataa aaagcatgaa
      661 tcgctgcttt gtaagtagag gacaagtcct cataaaaatc agatagatca ggataaatct
      721 ccttgatttt tgaagaacca tcattagata ggatctcttg tcgtaaaatc atcgtgggac
      781 tagggatcgt tgcttttgca acaaaagtat catctgtttc cactacttct ttcagaaaag
      841 caaaatcttt taaaaatggg tgatttggat tgaaagataa tttatctact acccgaatat
      901 tataagctgg tgctgctttt ccgtggaatg tttgattata tccagtttct ggaacatagc
      961 cttcgatacc atttagttct tctaaaaaat caagatgcca aaatccacgc cgaaattcac
     1021 catctgtaat tcctttgatg ccgactgctt tttggtcttc cactaatttt tttatttcct
     1081 ggtcttcgat tgcacgaagt tcttcttttg aaagtaagcc ttcttgaact ttttttcttg
     1141 cttctttgat cgattgcggg cgtaagaaac ttccgacatg atctactgta aatggtctat
     1201 ttttgctcat ttcactcact cttttctttg ttttttgtaa aaaaaaatcc ttttactagc
     1261 aaatactagt aaaaggacga ttatactcgt ggtaccacct tttttcagaa aaaacattct
     1321 ttctctcagc ctgacaataa aatcagtggt cttggtaacg aaagactgtt cgtttttgcc
     1381 tacctttcga caaaaaatcc tctaaggcca ttttcgacag tcaacttcta gctttttaca
     1441 ccaaccaaag cctctcttga accattttct gtctactttc cttctcatcg gatactatta
     1501 gaataaaatt agaatagcat gtttgaaaga aaagtcaata gggaataaga acaaacaaaa
     1561 actaatttgt tattcttagt aatttgtttg tggaatacaa taaagctttt ctaatatgat
     1621 aattgttcag tcgtcctttc atcttagatc acttccttaa aatgatcata cccgtttcaa
     1681 ccacataaaa aaggacttaa ttttgaaaaa tttcccatca aaactaagtc ctttttatta
     1741 tagttatgca aatttgctta ttttctcgct atttccgata catcttgaac aggagcaatt
     1801 cgactgaaaa cactggtgca aaagcattta tttgtcttat ggtagaaccg tggaaaactc
     1861 agactggtaa taaattgtca ttttttgaaa gaatagcgcc ctcttgaaca ttatcaatcg
     1921 cacttaccac ttgttgatct gactttgcat ataagtgacc gtaagttcct aaagtgattt
     1981 gaatattcgc atggcccaat cgttcttgaa tagatagcgg ttgaatattc tgctcaatta
     2041 gaaatgcaac atgagaatgt cgtagcgcgt gaagcttaat cggtttgaca cccgcttttt
     2101 tagcagcttc ttttaaccag ttcgcaaaag aataaggtgg cgtaaatgtt ccgtccaact
     2161 gagcaacata ttcaaaattt ccaatacgtt tttgcaagtc ataccactgt ctgagtttta
     2221 aggaaagctt cttccccaac ccaatcgttc gataagcaca tttagttttg ggagtcgaaa
     2281 agtaccaatc atcttttgta ttataaatca atgtcttctc aatcgttatc gtgtttttct
     2341 caaaatctac attcgaccaa gacaaagctt caagttctcc aatccgaatg ccagtataaa
     2401 agagcatttc atacacaatc ttccgatggt gggtttgaat cttatcatcc ttcaattcat
     2461 tcatcacttt ttgaaactca ctgtgttccc aaaactcaac tttcttattc tcagaaggaa
     2521 gcgaagaaac atcttcaacg ggatttttag caatcacccc ctctttaaca gcccgatcca
     2581 atacaatttt taggtgccct cgaatccgat tgatataaga ttttgataac gtttcgccat
     2641 taactttttt ccgtgaatta cgtgactctg tctggcttag ttcaaataac caatcctcaa
     2701 tatcgtgact tgtaatatct cgaacacgaa aatgatagaa aaactgcata cgcttttcga
     2761 aaatgaattg cgcattctca tatgtttgac ttttgacctg tctctcgtac cattttacaa
     2821 acctatcctt gtaaaactct tcgaaagtca tttgattggt agaacacagt tgattaggaa
     2881 ttgtcttgtt aaaatcatgt gtttccatgt acttgtcata agcaattttg gcatcttttt
     2941 gtgaagcata acctcgttta gtcttctgaa cctttcttcc attttcatct atacctagat
     3001 acgctcggaa gaaccatttt tttgtctttt tatcttggta aatattttta atttttggca
     3061 tcgttccatt cctccctgta cttgagaaaa gctactttaa tagtaccgcg cttgtactgt
     3121 ttattccgca acttgaatgt gaaaaagatg ttcaattact cgggatggca ccacaccaac
     3181 ttggcgatta ttataaaaat caataccttc aatccttgca agatatgcct tgctctcttt
     3241 aatcattcgt gcagcttgac taggtttaaa gcccatctct atcaaaattg cacgatttac
     3301 catgaaatca cattttgttt ccattctctc acctcctgat tctttactcc ttttaaaata
     3361 atcctcttta taatctattg tttctcattc gatggttgtg ttatactcgt cttgtcgagg
     3421 cggagtacgt tgcacaactt gttgtgtaag cagtactttg cttttttagt agcttgtttt
     3481 ctcatttgat atactttgta attttcgttc ataatactca acctcctcct gataatactc
     3541 cgggtctgcc ttcatatttt ggagaagttt gatctgtctt ggattcattt ctccttgttc
     3601 aataatcaaa ggcaacaagg aagaatgaaa cgtttgatcg acctgtgcaa taaaaactaa
     3661 tgatggtgaa acaccatgaa ttaaccaact catagttttc tcaaaacttt gaggaattgt
     3721 atctaaagaa atggtgattg cgccataatt gctaataaag gctgcccatt tggcatcaac
     3781 ttttgcacct agatcatttg gatcacggtc ataaaaacaa attgctccat taagtaactc
     3841 aaagaacagt ttggttaaat catgggtttt tgaaataatc tttgccaagc tttgcgcttt
     3901 ctcttttcga taccgtagtt caaagcgatt ctttactttt gcctcttcta aaggaattcc
     3961 tcgtcgcttc cgttgttcat ggtccttctg ataaaagcaa aaacgacact gactcttctt
     4021 tgagcctaaa tacaaggtct caccatttga ttcattgttt atgatatccg taccgccatg
     4081 agattcactc acttgaaaag tggtccacac atggcctagt attaccttat gcttaagttc
     4141 tggaatgctg agactcccca caaaatcatc taaagtaaaa tcaatccgtg taaagttccc
     4201 ctgataatct aataccttac gaaaaaactg ttgccaattt gtttttcgtg tttttagcca
     4261 tcttgctaag tgtttacagc cttgacctga aaactccatc acagttcctt taaccgcatc
     4321 gtcaatggaa taccgaatcg tgattacttg attccacacg tattggccaa tatagccctt
     4381 tggcgcataa tccagttcct caaaacgttc aatgggcatt tcaataatct cttcaattag
     4441 ctttaaatgt tgattcgaga aaaagtgaat ggttagataa tcaatcaaaa cacggttaac
     4501 tggaacgacc tcttcttgca aactatccaa cacaagattc aatttttctt cacttaattg
     4561 tctcgttcct ttttctactt tcgataataa ctatctcgaa atccccactt tttctgctaa
     4621 atatcgttgt gtccaccccc tttcttctcg agaaactcga atggcttcac cgagatcatg
     4681 ttgattaact tggttcatcg acaaacctcc ttctgctatt tacttgtcaa agaacctatt
     4741 gaacttattt taagtacatt tttattgtac gcctgtattt attttaagtc aatatgtttt
     4801 gacttttttt aagtacacac gtatactaaa tttaagaact tatcctgagg aggagttttc
     4861 atggagatag acaagcaagc agtcggcaat cgaattcggc agattcgcca agaattaaag
     4921 ttgagtatgg agaaatttgg caaactaatt ggtgatttac cgcgaagtac agtcaataac
     4981 tgggaacgtg ggatcaacct tccaaaaacc gaaacccttc accaaatcgc agagataggt
     5041 catgtcacca atgaatatct tttgtatggt gatcaagaga atcaatacat tttagaaatg
     5101 ctccaaaaaa aggcaggtaa gcttgatcct aagattgaag gtctcatagt agacgaaatc
     5161 aaacacgctg accttgttag tgaaaaagaa atggatcgaa tgattgaatt ttttattaca
     5221 aatctcattc caccaactga acaagaccag tttactttcc aatgtatcga tgaaaaaaag
     5281 cagctttatc ttggttcaac taattttggt aaggaagccc agctctattt gcaacatgat
     5341 aatcaaaatc atattctgca catgatgccc tttaccttct ccaactttag tgtggatcgc
     5401 atactcgtct atttagcaaa tcaaaaatcc tattcctatt ttgggaaaca tttacccaag
     5461 gaactaggtg agaaagccat ccttctatac tcaattaatc aattgaccag cgatgtgcga
     5521 attgccccgt tggtctattc agaagaaacc gcttcttatc agtacacaga ggacaatcaa
     5581 tatctactag aacagcaata tctttatcta ccattcgtga tggaagtcga gaaagaacgc
     5641 ctgttaaatg cagcgtatcc atcatcaaaa tgaggtgaca aaattgataa gcacgtgaca
     5701 aagaaaatac tgctaggaca ctccccaatg ggtagttgtc acagtttcct gttctttgtt
     5761 agccccctgt tagaaccggg ggctttattc ctgcagtccg gctacgcctt cctgcaatag
     5821 acaaaactcc agatcgggta attcgcttgt ggcgaaaccc gacctggagt ttgtctctgt
     5881 attttgaacc ctaaaaatta atcaaacaga ctgatattct cataatcagc gcgaaataag
     5941 ccattaagta cagttttcct acctattttt gataacccgc tggttagtta atattccttt
     6001 tccgagagag ataaatgatt ggttaaatct atatacatct caacctttaa aaatgttttc
     6061 gaaatacttc atcagtacat gtcatacaaa ttaccagtgc attctcaaat tctagttctt
     6121 taattgattt cagttgagga gcagttactt ttggcggaaa tttccatttt tgtccaaacc
     6181 tcttcccccg atgaaatttc tttgttgcaa ttattgcaat gaaaaatttt tgtttcaaaa
     6241 atatacataa ttacttcctc caataaaaac tatctacctc tcaaattatt tttctctaat
     6301 aagaaaaaca gaaaaggtaa taagatacaa cagtgatgat tcctaataaa gaaaagacta
     6361 caataatatt tttctaactt atcgccttca aagaaatagt ttcttatttt tcactaaaat
     6421 aaaaaattta ttgcaaaaaa aat
//
LOCUS       DANKAY010000135         6235 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000135 DANKAY010000000
VERSION     DANKAY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            95..1354
                     /gene="lysA"
                     /locus_tag="PWY98_002649"
     CDS             95..1354
                     /gene="lysA"
                     /locus_tag="PWY98_002649"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2038340.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1341..2141
                     /gene="dapF"
                     /locus_tag="PWY98_002650"
     CDS             1341..2141
                     /gene="dapF"
                     /locus_tag="PWY98_002650"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2038341.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2144..3025
                     /locus_tag="PWY98_002651"
     CDS             2144..3025
                     /locus_tag="PWY98_002651"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2038342.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3022..3738
                     /locus_tag="PWY98_002652"
     CDS             3022..3738
                     /locus_tag="PWY98_002652"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2038343.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3744..4439
                     /gene="dapD"
                     /locus_tag="PWY98_002653"
     CDS             3744..4439
                     /gene="dapD"
                     /locus_tag="PWY98_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2038344.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4455..5456
                     /locus_tag="PWY98_002654"
     CDS             4455..5456
                     /locus_tag="PWY98_002654"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2038345.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5453..>6235
                     /locus_tag="PWY98_002655"
     CDS             5453..>6235
                     /locus_tag="PWY98_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2038346.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADI"
BASE COUNT         2059 a         1088 c         1442 g         1646 t
ORIGIN      
        1 aaaattttgt ttaatattat tccaataaaa ttcaaatctt tatcaaatga aattccaata
       61 aaaacggaaa aaaagtgaaa agaggaatga aaaaatgaca gaattgataa ttggcggagt
      121 cacagcaagc acattggcag aaaagtataa gacacctctg tatgtgtatg atgaagaaaa
      181 gatgagacaa acgatgctgt cattcaagcg aggctttgta tcgaaacgtt ttgatacaaa
      241 agtgctttac gcctctaaag cattccaaac gattgagatg ctgcatttag ttcgtgaata
      301 tggattcggg ctagatgttg tcagtggtgg ggaaatctat accgctttgc aagctgattt
      361 tcctagagat actatctact tccatggtaa caacaagacg ccagaggaac tgatctatgc
      421 acttgaaaat gatttgttgc atattgttgc cgataatttg atggaagttg aattattagc
      481 agaattaagc caacgctatt taaaaaaaat ctgtatcatg cttcggttga atgtcggagt
      541 agaagcacat actcatgaat atatcgtcac tgcgcatatt gattcaaaat tcggcatgag
      601 ctatgaaagt aaagagtgcc aacaagccct tcagttgata aaagaaagca gctatctaga
      661 gttagaagga tttcatgctc atatcgggtc acaaattttt gatgttactg cttggttagc
      721 tgaaatcgac aaattgacga attacttaac cgattttgac caacctcttt ccttgaatct
      781 tggcggaggg tttgggattc gttatacaca aaatgatcag ccgatcccag tcgaagaggc
      841 actaaaaaaa ctgattgaat atacggaaga agctttggaa aaacgaaaac tgaccattcg
      901 tcaattactg atcgaaccgg gtagaagtat tgttggagaa gctggtacaa cactatacac
      961 gattggtttc atcaaaaaga caccacataa aaaatattac tttgtagatg gcgggatgac
     1021 agataatatc cgtccagctc tgtatcaggc agaatacgat tgtgatttag cagcaaaact
     1081 gtcggaagaa aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat caggcgatgt
     1141 ggtaattaaa gaagcttatt tgccgaaagc agaatcgggt gatcttttag ctgtttattc
     1201 cacgggagct tatggatact cgatgagcag caactacaat cgagcgacac gaccagcagt
     1261 tgtttttgtc aataatggcc aatcaagact gattgttcgc agacaagcat tttctgatct
     1321 actaaggggg gaagtttcct atgatccttc ataaatataa tggctgcgga aatgatttta
     1381 tcttattaga ttacgcagaa gacacagatt atagtcaatt agccgcttct ttatgcacaa
     1441 aagaaaaata tgatatagac ggattgatcg ccgtaaaaat cgatccgttg gaaatgatct
     1501 attacaacaa agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc tgttttgctc
     1561 gatacgtaaa tgatcagaac atgatgaatg aacaggaatt caacgtagag acattagcag
     1621 gcacaagaaa aattacggtg ttggatccct caccttttta ttgcagcgta gatatgggag
     1681 tgccagatga ttctccggaa aaaatcaaag ctaatcaaac ttatccaata aaaaaccagg
     1741 aaatagagat caatggtgaa aaagtaacga tcactagttt atttttagga acgattcata
     1801 ccgttgtttt tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt gaactgatct
     1861 gtcatcatcc tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta gtcaatgaac
     1921 aagaattgat cgtccgtacc tatgaaagag gagtaggttg gacacttgct tgcggtacag
     1981 gttgctgtgc ctcttatgtg gttgctaagg aaactgggaa aatttcagca gatcaggtag
     2041 ttgttcactt ggaacaagga gacttgatga tctctggtga ggatgcgata caaatggctg
     2101 gcccagcagt tcatgaatgg acaagaatat tggaggaatg aagatgataa aaggttcgat
     2161 cgtggcgctg atcacgccga tgaatgaaga gggatcagta gattatgctg gtttggaaaa
     2221 attgatccaa ttccatttag atgaacaaac agatggatta ttagttttag gtacaacagg
     2281 agaatcttct acgctcacac aatctgagga ggagcagatc cttcaactga cagtaaagaa
     2341 agtggctggc cgtgtgccgg tgattgcagg agctggaacg aataatacga aagaaacgat
     2401 tgaaaaagcg aagcattttg cttctttggg ggcagacgca cttttggtca tcacccctta
     2461 ctacaacaaa acaagtgatg caggactagc tgctcatttt acagcaattg cagaagcctc
     2521 accgattccg cttattttgt ataatgttcc ttctagaaca ggcatgtcga ttccaatcca
     2581 tgtattagta aatttagcag aacatcctaa tatcattggt ttgaaagaag cctcaggaga
     2641 tatggcttat gtgatggatg ccgctcggtt gattggagaa gaattcttcc tttacagtgg
     2701 aaatgatgat ttgatcctac cagtaatgag tgtaggagga agcggtgtaa taagtgtatg
     2761 ggcgaatatc caacctaaaa tagtccatga attagtaaaa gatacgcagg acggaagatg
     2821 gcagcaagca aaagaaaagc aattaaatgc acttgaactg atccatgcct tgttcagtga
     2881 gaccaatcct attcctgtca aagcagccat gtcattatta gacttacctt ccggacctct
     2941 acgcctgcct ttagtttctc tttcaaaaga gaaaaagaaa caattagctc agcttttgtt
     3001 aaaagaaagg acggttgaaa aatgaaaatc ggactgattg gcagtggaca gatgggaaat
     3061 agagtcgaag aagttgcaca agaaagagga gatacggtcc ttcttttacg gacggcacct
     3121 aaagaagcaa ctacgcttgc ttttacggaa ctgccagaac tgcttattga tttttctcat
     3181 ccagataatt tagagaaaat cctttcgtat agcttatcat atcaacttcc gttagtcatt
     3241 ggcacgacag gttatacaca atcacagttc caagaaataa aagaacatgc caaacatgta
     3301 cctgtgatgt atagtgctaa tttttcgttc ggcatcatgg tgatgaatca actaatcaag
     3361 caagcagctg caatgttgca aggctggcag atcgaactga ttgaaaaaca tcatagtcgt
     3421 aaaaaagatg caccatctgg tactgcaaat atgctgcttc aaacaatcca agaagtgcaa
     3481 aagctgcagc ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca aattggtgta
     3541 cacagtattc gtgcaggttc tttacctggt gaacatgatg tattgtttgc tgctactgat
     3601 gaaatattta cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc aggcgcagta
     3661 gaagcagcgg actggttgaa agatcagtca ccaggttatt acaaattaga agatttatta
     3721 atggacaaag gggtgtaaaa aacgtggaag ctcaagaaat catccgctac attcaaacgg
     3781 catctaaaaa gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc gaatttaaag
     3841 aaagtaaagt ttttggtgaa ggaagcagct tcgttgtttt tggtgactat gagacgattg
     3901 ctccggtttt agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa actgctgcaa
     3961 gatacagcgc agttcctttg ctagatatta aaaaaattaa tgctcgtatc gaaccaggtg
     4021 ctattatcag agatcaagtg tcaatcggca acaatgccgt gatcatgatg ggagcgatta
     4081 tcaatattgg tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct gtcttaggtg
     4141 gacgagcgac agtgggcaaa aactgccata ttggtgcagg agctgtctta gcaggtgtga
     4201 tcgaaccagc atcggccaaa ccggttatcg tagaagacgg cgtactggta ggtgcgaatg
     4261 cagtgattgt tgaaggtgtc catatcggaa aagatgcagt cgtggcagca ggagcagtgg
     4321 ttttagaaga tgtggcagca gaaactgttg tcggtggaat acctgcacgt gtattaaaaa
     4381 taagtgatga tcagacaaaa gaaaatacag cattgattgc tgcgttacgc gaattataga
     4441 ggagatggag aaaaatgcgc gaaatagata cctgtattgt gggggcaacc ggattagtcg
     4501 gccaaacgat gctcaaagtg ttagaagaat atgattttcc agtaggacga ttgaaattat
     4561 tagcttcaaa acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa cacactgtac
     4621 aggaattgac agaaacttca tttgaaggat atgatttagc gctattttct gcaggtgctt
     4681 ctgtatccaa agagtttgcg ccaatcgcac gaagtcaggg actatttgtc atagataatt
     4741 cctcagcatg gcgggaagat tcggatattc cattaatcgt tccagaggtc aatctggaag
     4801 actatacatt gaatcggctg attgcaaatc caaattgttc aaccattcaa gcggtacttc
     4861 cgcttaaagc tttacaagaa gcctttggta tccagcgagt caattattcg acctatcaag
     4921 cagtttctgg ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa aatgacgagg
     4981 aaccagtttt gtatccttat aatattagcc aaacggtgat tccggaaatc gatcagcctt
     5041 tggaaaatgg gtatacaaaa gaagaacaga aaatgataaa tgagacccgt aagattctgc
     5101 atcagcctgc tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa aatggacatg
     5161 gagtctctgt ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt agagagtgtc
     5221 tcagccagtt tccagggatt caattagaag atgatctagc agcacataaa tatccgacga
     5281 gcattcttgc tagaggaaca gatatggttt atgttgggag gatcagaaaa gacacttctt
     5341 tagagaacgg attgttgttc tatacgactg ccgataacat acgcaaagga gccgctgcga
     5401 atgccgtaca gattgctgca gctttaatgc agaaaatagc ggaggtggca agatgagtaa
     5461 tacgctgaaa gtagcgaaat tcggcggaac atcgatgtct catgaagagc aattcaaaaa
     5521 agtccgtaca attgttcttt cagatcccaa tcgccaagtg atcgttgttt ctgctttagg
     5581 caaacgtacg tcagatgatg acaaagtaac agacttactt tatttgattt atgcttacct
     5641 tcagcatcat gttgattgga cacccctttg gaaaaggatt tgtgatcgtt tcattcaagt
     5701 aagagatgtt cttgccctgc agttacctat tgaagatgaa ctagcacgca tacagaaaca
     5761 actggaagac gaaaagataa ctgaagatta tttagtaagt agaggtgaat atctgactgc
     5821 tcaactgatg gctgagtatc ttggttacca gtttattgac gcaaaagacc tgattttctt
     5881 tgactatgat gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa aagcatttaa
     5941 aagatatccg aaaatggttg ttccagggtt ttacggggcg aaccctgcag gaaaagaaaa
     6001 attattgggc cgtgggggaa gcgacattac cggcgcgata ctggcgaaga tgttgcaagc
     6061 ggaaagttat gagaattgga cggatgtttc aggaatcatg atggctgatc cgcgaatcat
     6121 cgatcatcct aaaatcatcg aggaaatctc ttttagagaa ctgagagaga tggcttatat
     6181 gggggctaac gttcttcatg aagcagctgt tttcccagtt caagaggctg atatc
//
LOCUS       DANKAY010000136         6200 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000136 DANKAY010000000
VERSION     DANKAY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1087
                     /locus_tag="PWY98_002656"
     CDS             <1..1087
                     /locus_tag="PWY98_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2038347.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="PWY98_002657"
     CDS             complement(1305..2447)
                     /locus_tag="PWY98_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2038348.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="PWY98_002658"
     CDS             complement(2452..2637)
                     /locus_tag="PWY98_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2038349.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="PWY98_002659"
     CDS             complement(2991..3236)
                     /locus_tag="PWY98_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038350.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="PWY98_002660"
     CDS             complement(3230..3631)
                     /locus_tag="PWY98_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2038351.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PWY98_002661"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PWY98_002661"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2038352.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..>6200)
                     /locus_tag="PWY98_002662"
     CDS             complement(5649..>6200)
                     /locus_tag="PWY98_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2038353.1"
                     /translation="VPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGF
                     EVEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRI
                     VWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKL
                     MGLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1996 a         1176 c          869 g         2159 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac
//
LOCUS       DANKAY010000137         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000137 DANKAY010000000
VERSION     DANKAY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            725..811
                     /locus_tag="PWY98_002663"
     CDS             725..811
                     /locus_tag="PWY98_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2038354.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWY98_002664"
     CDS             complement(932..1621)
                     /locus_tag="PWY98_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038355.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWY98_002665"
     CDS             complement(1628..1906)
                     /locus_tag="PWY98_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038356.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWY98_002666"
     CDS             complement(1866..2780)
                     /locus_tag="PWY98_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2038357.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWY98_002667"
     CDS             complement(2762..3142)
                     /locus_tag="PWY98_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2038358.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWY98_002668"
     CDS             complement(3592..3879)
                     /locus_tag="PWY98_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2038359.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWY98_002669"
     CDS             complement(3899..4123)
                     /locus_tag="PWY98_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2038360.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWY98_002670"
     CDS             complement(4578..4748)
                     /locus_tag="PWY98_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038361.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWY98_002671"
     CDS             complement(4763..5296)
                     /locus_tag="PWY98_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2038362.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWY98_002672"
     CDS             complement(5317..6159)
                     /locus_tag="PWY98_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2038363.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANKAY010000138         5956 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000138 DANKAY010000000
VERSION     DANKAY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            89..562
                     /locus_tag="PWY98_002673"
     CDS             89..562
                     /locus_tag="PWY98_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HDL2038364.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            636..1076
                     /locus_tag="PWY98_002674"
     CDS             636..1076
                     /locus_tag="PWY98_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2038365.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            1089..1298
                     /gene="copZ"
                     /locus_tag="PWY98_002675"
     CDS             1089..1298
                     /gene="copZ"
                     /locus_tag="PWY98_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2038366.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            1298..3484
                     /locus_tag="PWY98_002676"
     CDS             1298..3484
                     /locus_tag="PWY98_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2038367.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            3627..5783
                     /gene="copB"
                     /locus_tag="PWY98_002677"
     CDS             3627..5783
                     /gene="copB"
                     /locus_tag="PWY98_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2038368.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         2040 a         1033 c         1332 g         1551 t
ORIGIN      
        1 tttttttgcg gcgttataag tggtatgatc gaaccaaatt ccaaagagaa gtgagggaga
       61 agaatgaaga tcatcgtgat tggacaaagt gggtcgggaa aatcgtccct cgcaaggaag
      121 atcaaagaaa tcaccggctt tccgcattca ggcaaaaaag tggtttttgc aagaacagca
      181 atcgtttatg gtatctaatc cgtcatggat cgttgaagga aattatactt ctacgttaga
      241 ggaaaggatt aaagaagcag ataagatcat ttggcttaaa gtgccgcgtt atctcgccat
      301 ctaccgagta gtttggcgtt ctctaaaaag aaagataaac aaaaaatctc ggccagatat
      361 gcctgaatca ttttcggaga aactaaacag gaaatactta gaatttttat cttttatctg
      421 gcattttgaa ggaaacaatg aaccaaaaat acagcagctg atccaagtag ctaatgcaag
      481 aaataaactg atcatcctta aaacaagacg agagaaacaa gaatttttaa ctaaattgaa
      541 aatagagagc aaaaataatt gacagttttt gtttacaagt gtaatctgta ttcataagtt
      601 aatgattaca tttgtaaacg aaaggtgtga aataaatgga aaaagtcaaa acgccagtaa
      661 agattagtga ttcagagtgg gagatcatgc gtgttatttg gacacttgga cagacgacag
      721 cccaagaaat cacgcagatc ttagctgatt cgatggattg gaaaccagca acgatcaaaa
      781 ctttgctggg tcgtttggta aaaaaagaag tgatctggac agaacaagaa ggaaaaaaat
      841 tcatttatca tccagctgtc tcggagatgg aaacagttcg cagtgcgacg gaaaatcttt
      901 tttcccatat ctgtgcaaaa aggattggag agacgattgc ggatctagta gaagaagcca
      961 ctttgactca agaagatatc aatgagattt tgaaacaact ggaacaaaag caaccagtag
     1021 atacgatcga atgtaactgt atcccaggtc aatgtgaatg taaacaacat caataggagg
     1081 aagaaaaaat gaaacaacaa ttttcaatcg aaggtatgag ctgcaatcat tgtgcagcaa
     1141 gagtagaaga aacagtaagc gcacttgacg gcgtgcaaaa agtgaaagta aatttgaaaa
     1201 aagcaaacgg tacggtcaag tttgatgaaa caaaagttca atccgaaaag atttgtcaag
     1261 caatcaatgg attgggttat aaagcagagg tgatttgatg gcaacagcag aaaccaaaat
     1321 ggatacgttc gtcattacag gtatgacttg tgcaaattgt tctgctagga tcgaaaaaga
     1381 gctgaacgga caacctggtg tggtcaatgc cacagtcaac ttggccactg aaaaagcaag
     1441 tgtcaaatat gaagggacga ccactgaaaa attaatccaa agcgtagaaa acatcggcta
     1501 tggtgcaatt ttgtacgatg aagcccacaa acaaaaaatt gcagaagaaa agcaagcgta
     1561 tttgaaaaaa atgctattcg atcttatatt gagtacggtt ttgactttgc cgttgatgtt
     1621 gtcaatgatt gctatgatgt taggaagcca tgcggcgatt gttcactttt ttcattttcc
     1681 aatcgtccag ttagtgctgt ctgctcctgt ccaattttat gtcggtgctc gtttttataa
     1741 aggtgcgtac catgcaatca agtcgaaagc accaaatatg gatgtattgg tagcaattgg
     1801 aacatcggct gcctttgcat tgagcattta caacggattt ttccgtggac acccccaaga
     1861 tctttatttt gaaagcagca gtatgatcat cacgctgatt ttgctcggca aatatttgga
     1921 acatacagca aaaacgaaaa caggtaatgc aatcaaacaa ttgatgtctc ttcaaacaaa
     1981 aaccgcccaa gtgataagaa acgggaaaga agaaacgtta gcgattgaag aagtagtcgt
     2041 tggtgatcaa ctagtcatcc gtcctggtga gcagattcca gcagatggtc gaattatttc
     2101 aggaagcagt gcgatagatg aaagtatgct tactggtgaa aatcttcctg tagaaaagaa
     2161 tccggatgac acgctttttg gcgggacaat caatacgaat ggtctgctac acatggaagt
     2221 cacacaagtc ggcaagcaga cagtattagc gcaaatcatt caaatggtgg aagatgctca
     2281 agggagcaaa gccccaatcc aaaaaatcgc agatcggatc tcagggattt ttgttccgat
     2341 cgtattagtc atcgcgttta tcacgctcat agctacaggt ttgatcactg gtgattggca
     2401 actcgcattg atccatagtg tctctgtatt agtcattgcg tgtccatgtg cattaggttt
     2461 agcgactcca acagctatta tggtaggaac gggtgttggt gcacgaaatg gaattttaat
     2521 taaaggcgga gaagcattag aagcagctgc tcacttagac agcattgttt tggataaaac
     2581 tgggacgatt acagaaggaa agcccaaagt tacagatcta gtcggttcta aagaagtcct
     2641 atctattttt tatactttag aacaagcttc tgaacatcca ctaggtaaag caatcgtgga
     2701 atacggaaaa ctgcaagaag cagcaacata cgatatgatt gattttacag ctcacccagg
     2761 tgctggaatc agcggtacga ttaacggagt acgctatttt gcgggtacac gtaaacgatt
     2821 aattgaattg aacctatcat ttgatgaata ccaagaacac gcgttagaat tagaacagca
     2881 gggaaaaaca gtcatgtttt tagctgatga aaagcaagtg atcgggttga ttgccgtggc
     2941 tgatcaaatc aaactagaag ccaaacaagc aatcaagcag ctccagaata aaggattaga
     3001 tgtttttatg ctgacaggag acaacaaact ggcagccgaa acgattggta aacaagttgg
     3061 tattgatcca aagcatatct ttgctgaagt acttccagag gataaagcag cctacgtaga
     3121 aaaacttcag aaagatggca aaaaagtcgg tatggcaggc gatggaatca atgatgcacc
     3181 ggctttagca ttagctgatg ttggtatggc aatgggaagt ggaacggata tcgcgatgga
     3241 aacagccgat gtgacgctga tgagtagcag tttggcatcc attgcacaaa caatcgagtt
     3301 gtctagggtg acattgagaa aaatcaaaca aaatcttttc tgggcttttg tctataatac
     3361 gattggtatt ccttttgccg cactaggctt tttgaatcca atcattgcag gaggagcgat
     3421 ggcgtttagt tccgtcagtg tattgttgaa ctccctgagc ttaaatcggc acatgaaaaa
     3481 gtaagagaag ggatggagta aaaatgaatg ggaacgatat ggagaaaaaa catgaacata
     3541 aaaaagaagt agtaaacaaa gaaagtacag aatctcatga aaaacagatg gagatgaaac
     3601 atgaccatag ccagatggac cattccatgc acatgggaca tgaacataaa aaagaagtag
     3661 taaacaaaga aagtacagaa tctcatgaaa aacagatgga gatgaaacat gaccatagcc
     3721 agatggacca ttccatgcac atgggacata accatggaga tatagcaaac agcaaacaaa
     3781 tggaccattc gatgcacatg gatcacgatc atggcggcat ggatcattcc atgcacatgg
     3841 gaaatttcaa acaaaaattt tggctatcgc ttatcctagc tatcccaatc attctttttt
     3901 caccaatgat gggaatggaa tttccatttc aagtaacgtt tcctggttct gattggctcg
     3961 tattgatctt agcaactata ctatttatct atggcggtca gccattttta agtggagcta
     4021 agatggaatt gaaacaaaaa agtcctgcta tgatgacact gatcgcgatg gggattacag
     4081 ttgcatatat ttatagtgtc tattcgttta tcgcaaatct gctcaatcca catacacacg
     4141 tcatggattt cttctgggaa ttggctactt tgatcgttat tatgctacta ggtcactgga
     4201 ttgaaatgaa tgctgtgtcg aatgccagca atgctttaca aaaactagca gaattactgc
     4261 cagaatcagt aaaacgcttg acgaaagatg gaaaagaaga gacagtttca ttgaaagaag
     4321 taaacgaagg tgatcgtttg atcgtccggt ctggtgataa aatgccaaca gatggtgtaa
     4381 tcttgaaagg tgaaacaatt gttgacgaat cagctgtgac tggcgaatca agaggaatca
     4441 aaaaacaagc caatgacaaa gtgattggcg gttctatcaa tggggatgga acgatcgaaa
     4501 tcgaagtaac tggaactggt gaaaatggtt atctagcgaa ggttatggaa atggtgcgaa
     4561 aagcacaagg agaaaaatcc aagctagagt cattatctga caaagtggca aaatggctgt
     4621 tttatgtcgc attaattgtg ggtatcctgg cattcatcgc ctggttgttc ttgacagatc
     4681 ttcctaatgc attagaacgt atggtaacag tctttatcat cgcctgtcct catgctctag
     4741 gtttagctat cccattagtt gttgctcgct ctacatcaat agcagctaaa aacggattac
     4801 ttttgaaaaa ccgcaatgca ctggagcaag ccaatgatct agatgtgatc atgctggaca
     4861 aaacaggaac actaacagaa ggaaaattta ctgtaacggg tgtcgaagta cttgatgatg
     4921 cgttcaataa aaatgaaatc cttcaatatt taggagcatt ggaagcgaat gccaatcatc
     4981 cattggctgt tggtattatg aattatttga aagaacatga aatcaagcct tatcaagccg
     5041 aaaatctaaa aaatctttca ggagttgggc tagaggcaac agtcaaaaac caacaagtga
     5101 aaatagttaa tgaaaaagaa gttgagcgtc tcggattaaa cgtagaacaa gcattattga
     5161 aaccttacca agaacaagga aataccatca gcttcctgat tttagaaaat catctagctg
     5221 caatcgttgc tttaggtgac gtagtaaaaa cagaagcaaa agaatttatc cgaacattga
     5281 aagaaagaaa aatcactcct gtcatgctta ctggtgacaa taaaaacgct gcccaagcag
     5341 tagcagatta tttagggatc gaagaatatt atggcggttt attaccagat gataaagaag
     5401 ctgttgtcca gaaatacctt gaccaaggga aaaaagtaat catggtgggt gatgggatca
     5461 atgatgcgcc aagtttagcc cgtgcttcaa tcgggatggc aatcggtgca gggacagata
     5521 tcgcaatcga ttctgcggat gtcgtattga caaacagcga tccaaaagat attttgcatt
     5581 ttcttgatct agccaagcaa acgcgaaaga aaatgatcca gaacctctgg tggggggcag
     5641 gttacaatat tcttgcaatt ccgctagcag ccggaattct tgcgccaatt ggtatcatat
     5701 taagtccagc agttggtgct gtcttgatgt cgttaagtac ggtagttgta gctctaaatg
     5761 ctttgacatt gaaaatcaaa taataatgaa aaaaggttgt gacaaaaaac gtgtcacaac
     5821 ctttttttcg aataatcgat actcaagcag ctggttctcg gcattccaga agtagattct
     5881 ccagaaattt tatcttattt tttgaagcgg ctcccttttg tcagaacttt tttgtatacc
     5941 gtttttctta ttatat
//
LOCUS       DANKAY010000139         5947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000139 DANKAY010000000
VERSION     DANKAY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(102..1028)
                     /gene="glsA"
                     /locus_tag="PWY98_002678"
     CDS             complement(102..1028)
                     /gene="glsA"
                     /locus_tag="PWY98_002678"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2038369.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1051..2478)
                     /locus_tag="PWY98_002679"
     CDS             complement(1051..2478)
                     /locus_tag="PWY98_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2038370.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2509..3723)
                     /locus_tag="PWY98_002680"
     CDS             complement(2509..3723)
                     /locus_tag="PWY98_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2038371.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4064..4306)
                     /locus_tag="PWY98_002681"
     CDS             complement(4064..4306)
                     /locus_tag="PWY98_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2038372.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4338..4895)
                     /locus_tag="PWY98_002682"
     CDS             complement(4338..4895)
                     /locus_tag="PWY98_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2038373.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(4908..5096)
                     /locus_tag="PWY98_002683"
     CDS             complement(4908..5096)
                     /locus_tag="PWY98_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2038374.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5109..5675)
                     /gene="amaP"
                     /locus_tag="PWY98_002684"
     CDS             complement(5109..5675)
                     /gene="amaP"
                     /locus_tag="PWY98_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2038375.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1796 a         1256 c         1023 g         1872 t
ORIGIN      
        1 aaaagaaact gggacaaaag tcaaacaagc tcacaatttc agcctcattg taggtagctt
       61 tgagccgttt ggacttttac cccagcctct ttcaagaatg gttattcaaa gatgtcagcg
      121 tctagttctt tgactacatc tttcaacaat tggataccag ctgcgctgtt accaaatgga
      181 tctagtgccg ggctgaagac acctatgccg taacgatttg gcacagctgc catcaacccg
      241 ccgcctacac cgctttttgc aggtacacca acatggacgg aaaactctcc tgattcatca
      301 tacaaaccag cagttgtcat aattgatttg acgattgtag cactttcttc agtaatgatt
      361 tgtttgccat tccaaggagc aattccttta tttgccagca cagcagctaa ttttgctatg
      421 cctgtagcgg tgacattgag ggagcattgt ctgaaataag catcgagtat ctccggtacg
      481 tctccttcga tcatttgatt acctttcata tagtaagcaa gagagcgatt gatatcacct
      541 gttcttgatt cagaatgata gatttcttca ttcaatgtga tattaggatc atcacagatc
      601 tctttcataa atgacaagat tcgctcaaat ttttcatctg catctttacc ggcaatcaac
      661 gaggttgttg caatcgctcc tgcattaaca aaaggattca ttggatgttt gcgatgattg
      721 atttccatat tcatgataga attgaaggca aaaccggttg gttccgtgtt gattgtttga
      781 aagacttttt ctattccgtt gtctttgatt gctaataaca atacgggaac ttttgacatg
      841 ctttcgatgg caaagcgcac atcggcgtct cctccctcaa tcagttgttt tgtatccaaa
      901 tcataaatcg ccatacctaa ttgtttggga tccacttcag ctaatgctgg aatgtaatcc
      961 gccacttgtc ctgtttgata atagtggaaa ttttttccaa tcaattgttc taattctttt
     1021 acttccattt gaataaccag ctttcatttt ttatttttct gcatgctgga atttatgacg
     1081 gcatgcgtag atcgtaaatg gaataactaa gacgataaga taagaaacaa tcagaatcgt
     1141 ttcataaatc ccatgctgtc cagctgcgat actagcaggt ggaacaaatg agatacaaaa
     1201 agcaaaaagt gaagtcaaaa gacctaaaca agcaacgatt acttttacaa cttttccgcc
     1261 tggaacctga taggctcttt caagactgtc atctttaaaa accagcttca aatagcccgc
     1321 gaaaaacata aagtacaaca tcaggtagat tacaacagtc aatgacatcg ctgcaagaaa
     1381 tgaaagatta ttccccccgc cgcttaatgt aaagacggct gcccagattg ttgcaacgat
     1441 cccatttgcg atcatcaaat gagcaggaac accgtgtgtg ttttcttttg caaaatattc
     1501 aggaagtaat ccttcttctg ctgctacgtg aagtgctttt gaaggagagg ttacccaaga
     1561 agcaatttct ccgatgacac ctaaacaaac aagaatcgca agaattttcg caatccatgc
     1621 aagatttgga tcaatgtgtc ttaataggaa ttctaacgat tgaatcaccc ctgtattaag
     1681 agacaaacca tgttgaggta ctgtagtagc gacggtcaat ccgccaaaag tatctaaaat
     1741 gattgccaga ataaccaata caaacattgc taatggataa tcctttttag ggttttccat
     1801 ttcatttgca tgactggcag atgtttctac gcccatatat gctaaaataa aggaaacaaa
     1861 aacaaccagc gtaggcaatt tcgaaaaatc aggaatcaat gttttcatag acagtatcgt
     1921 ttgtagtgga gcgcctgaag cgacataatg gatccctaag ccaaacaaga ctaatgctgg
     1981 caacaagata ccagctacaa aaccgatccg agcaatcaat gaagtgtatt tggttccttt
     2041 taactgtgag agtaaaatag cccagaaaat aatcaatagg attgccaatt tcagatattt
     2101 attgttttgt atagcaccaa tacccgttgc ataggataat gcaccagtta agaaatacag
     2161 catggtcaag tagccaactg ttatctcaaa ccattggaaa aagattgcgg caaaaccgaa
     2221 tcgttttccc aatgtgcgac tgacccaagt atagacaccg cctttttccc atcctttcac
     2281 cgttgccatc tctgctgcgc acaatgccac agggataaac catagcaaac cgcctaataa
     2341 caagaaaaat actaagctga agcctgaggt cgcgaatgtg ggatattcat ataatgaaac
     2401 gaccattgag atggtcatag ccaaaaagcc aaagaacgtt aatttttttg caggaatttt
     2461 cgttttgctt tgttgcatga atcttcctct tttctatttg aatttgtttc aattgagttt
     2521 gttgccaaag aagaaaaagt ccaatccttc ttcttgttct tcttttgtag tagtgatcgt
     2581 tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc acataggcat acacggcagt
     2641 aatgatgaca ccaatcactt gaatccctaa gaagtaccag ctgcttggat tcgttagtaa
     2701 atggctgtta gatgcaaaga tccctatcag aatcgtacct gtgaatccgc ccatgccatg
     2761 aacaccccac acatcaagtg catcatccca ttgtttcttt ttacgaaaat caacagcagc
     2821 atgacagacg atacccgcaa tgatcccaat cagcatggca ctttttgctt ctacataacc
     2881 ggcacaaggc gtaatggctg ccaatccagc aacggatcct gttagtacgt cgacaaaatc
     2941 aaagccattt ccttttactt ttgcaatgat cagccaagta atcatcgcaa atgcaagagc
     3001 gatgaatgtt gaaccaaatg cattgactgc ttgttcatct gctcgaagtg caccaccgga
     3061 attaaaaccg aaccagccaa accaaagcag tcctgtaccg atagcagctg ccataagact
     3121 gctatgctga cttctttctc ctaaaggaat ctttctttga ccaagtgtga ggatacatgc
     3181 taaagagccg aatccagctg tcgtgtggat gactgctcca ccagcgaaat ctctaaatcc
     3241 catctgatcc aaaaatccgc caccccatat ccagtggcaa acagggaaat aaatcagcag
     3301 attccaaaaa ataacaagta taatatagcc ttttaaattc aatctccctg caaaagctcc
     3361 tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg taaagaaaga acattaaaaa
     3421 tggaatcgtt gcaccatgaa acatgttggg gaagcttgta acatgtctca tcaaaaagaa
     3481 atctgccggg tttccaataa caccgccgat atcccgtcca aaggccaatc cgaatccacc
     3541 aaatacccac ataatcgttg ttatcccaac ggagagaaaa gattgagaca tgatcgttag
     3601 tatattattc ttccgtgcta atccgccgta aaaaaaagct agaccaggag tcatcaggca
     3661 aaccattgct gtacataaaa tcatgaatgc tgtatctcct gtattgatag gtaatgtatt
     3721 catttttttc gcctcctttc tacgattacg agtataaaag aaggtgtagt tgtttttttc
     3781 ttctgttttt ttgaattaat acttgcattt ataaaagaca gacaattaat aaaagcttga
     3841 tgtgttgtgc tctttttcgt ttttcacaca aataaagaca aaaaataaac ggtcttcttt
     3901 ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa agatattttt tcataaggaa
     3961 taaagaatac ttcatataaa atatataaaa aagaaccgca gcgagtgtcg ctacggttct
     4021 caaatttgtt cttcatttca acataagttt ttcttcaatt tgcttataat ttacgtgcaa
     4081 agaatgaaac gactaatact aaaatgattg caccgatcaa tgatggaatg atagccattc
     4141 ctgcaattgt tggtccccaa gttcctagta aagcttgtcc aattgctgaa cctactaaac
     4201 cagcaatgat gtttgcgatg atccccattg atgatccttt attcgtgatt gctcctgcaa
     4261 ttgctccaat gataccgccg acgattaatg cccaaataaa tcccatttta aattcctcct
     4321 aactgacttc ttacttctta ttgtacacgt ggttcgttat tttctttgac tttttgtgca
     4381 cctttgccta cagcagattt tgctttatct gtttggcgtg aagcaaattg tcctgtggat
     4441 tttgctgctt ctgttacttt gtcttgtact gtttcttgat ctttttggta ttcttcttgt
     4501 gttttgatgt ctgcaacatt cacattcact tcgataacat ctaaatgtgt catattgttt
     4561 acttcagtac tgatcaattc tttgatttgg ttgtaaatat cttcgatgtc tttaccgtat
     4621 tctacgatca catctagatc cacagctact tgtttcttac ctacttctgt attgatacct
     4681 gctgttacgt tatctgtgtt gactaatttt tcagccatat ttgagaaaaa tccgccatct
     4741 aatgtcaata gaccatctac atctttcaat gcataaccga tgattttttg gatgactttg
     4801 tcttcataag tcaattcacc attcatcatg cggttttctc ctttttcatt tgccaaaact
     4861 gatccatttg ttgtattcat gtttttgttt tccatctttg tttcctccta tgatttaaaa
     4921 aattgatcaa ctattcctga ctgtttcaaa taaaaaccga ttcctgcgcc aattgctgtc
     4981 atgattatga tcaacaatgt cttgaaaaat ccaatcgtta accataaaac agctagcagt
     5041 agtccaagac ccccaaagat aatagggagc ttgtatgctg aaaatagttc tttcattatt
     5101 tggccctcct attccactcg agagtgcttg tagtctcttg tatcttcttg ttcttcataa
     5161 ccgctgtatg tgactgccac tttgaccgtt tcttttgttc cgagtacttt agttacttct
     5221 tcttcgatat cttgcatcaa tacacctgtt ttccctacta atgaagaagt tcgtttcaag
     5281 tctccgttga catggatatg gattcgattt ttcgttgaac gtatccgtac tttaggtgaa
     5341 tgaatgaact cttcttcgtg taaatgggaa cgtaccaacc cttcgatcgc ttttttgtcg
     5401 attgttagtt tgccatcttc ccgtttcata acgaaagttc ctcttgactt tggatagaaa
     5461 agaacgacta gcaagatgat aatagcaaga acggcaaata ctacagctgc ccagaaaata
     5521 tattgttgca tgtagtaatt cgttaaagta aagaagcgaa atcgttccaa ttgttcaggc
     5581 ataatatacg gcgcattgat caacgcaatc gctagaaata ctgaaagtag cagtaaactc
     5641 atgataacac cgattatttt taacccctta ttcattttcc acctcctata aacttatttt
     5701 taccctgtaa ataactctac cgacaatcac agtatagata gcgaacagtt agcatagcat
     5761 cagtaaataa agcgctacca aacattgctg gatcataacg gcgttttatt gccagtctga
     5821 tactgaaata acaattgctt acttattctc tatttacttt tgattgctgg ctatacttaa
     5881 aggatattat tcaagattaa aaattgcaaa gattatgctc agttattatt acaagattct
     5941 atttatt
//
LOCUS       DANKAY010000140         5885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000140 DANKAY010000000
VERSION     DANKAY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(14..838)
                     /gene="nadE"
                     /locus_tag="PWY98_002685"
     CDS             complement(14..838)
                     /gene="nadE"
                     /locus_tag="PWY98_002685"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2038376.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(850..2322)
                     /locus_tag="PWY98_002686"
     CDS             complement(850..2322)
                     /locus_tag="PWY98_002686"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2038377.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(2959..3321)
                     /locus_tag="PWY98_002687"
     CDS             complement(2959..3321)
                     /locus_tag="PWY98_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2038378.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3544..4296
                     /locus_tag="PWY98_002688"
     CDS             3544..4296
                     /locus_tag="PWY98_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2038379.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(4436..5359)
                     /locus_tag="PWY98_002689"
     CDS             complement(4436..5359)
                     /locus_tag="PWY98_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2038380.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(5374..5646)
                     /locus_tag="PWY98_002690"
     CDS             complement(5374..5646)
                     /locus_tag="PWY98_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038381.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1770 a         1182 c          966 g         1967 t
ORIGIN      
        1 ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat
       61 gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc
      121 cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca
      181 ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat
      241 tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt
      301 cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg
      361 tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac
      421 gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt
      481 gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa
      541 agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag
      601 cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg
      661 ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa
      721 gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt
      781 ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt
      841 attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat
      901 agccatttta tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa
      961 gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc
     1021 gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg
     1081 gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa
     1141 gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt
     1201 cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc
     1261 tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac
     1321 tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac
     1381 accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa
     1441 gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac
     1501 acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt
     1561 gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata
     1621 agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata
     1681 atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa
     1741 tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt
     1801 tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc
     1861 ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac
     1921 catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat
     1981 caatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa
     2041 ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta
     2101 agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc
     2161 tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc
     2221 atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc
     2281 cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg
     2341 gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca
     2401 ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc
     2461 taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca
     2521 atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta
     2581 gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat
     2641 gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt
     2701 gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc
     2761 gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt
     2821 taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca
     2881 tcaatcagtc tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa
     2941 gatgattatt agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat
     3001 atctttattt ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac
     3061 atggactttg acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc
     3121 ttgccttgtg atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc
     3181 taccaatagc agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt
     3241 cccaaaaaag aattccacta ctttaggata aaatgtctta atatttaaat tactattcac
     3301 aggtgtttcg attagcttca tctcattcct cttttctata tagtcgattt agatccaatc
     3361 ttaagttatg ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag
     3421 acaaaaggat ttacctttta tcacttaata gtataacata atttcatgag aaaaaaatac
     3481 aattttttct tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa
     3541 ataatggagt acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg
     3601 cgcttttatg atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg
     3661 atttatggca aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca
     3721 ttcaagttgg atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct
     3781 ttaatcaagc atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca
     3841 actgtccaac agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa
     3901 tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt
     3961 cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat
     4021 aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt
     4081 tacttaaaag aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag
     4141 tggttatctt acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg
     4201 tacgaagccg atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca
     4261 aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa
     4321 gtacacgtaa aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa
     4381 cttttggtgt ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc
     4441 gttgaataaa cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa
     4501 atgccatttc tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc
     4561 tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca
     4621 agatgcctgc tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa
     4681 aaaagaagcc tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt
     4741 catcaaaaga tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta
     4801 gcctgccact tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag
     4861 taagaccttc tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct
     4921 tgatttcttc acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg
     4981 tattggcacg agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat
     5041 accttcgctc tttttctttc tgatagcccg tcggtaaaag tgtcactttg taatcataat
     5101 caaacgctgc ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag
     5161 gcagaatcac tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag
     5221 ggatataatc gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat
     5281 atggatgtcc attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga
     5341 tatctcgaat gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata
     5401 gtctaccaca gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact
     5461 gatccgctgc ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat
     5521 ttcttttact atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt
     5581 gaactcgaca aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt
     5641 attcattgca agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc
     5701 aaggagtttg ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt
     5761 attttaaatt gaatacatta taatgatact gtaaatatat ctttattatt agtgaatgga
     5821 tatttacttt taatagataa aatattataa ctattaaaaa taactatttt cattataatt
     5881 acaca
//
LOCUS       DANKAY010000141         5770 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000141 DANKAY010000000
VERSION     DANKAY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            14..1168
                     /locus_tag="PWY98_002691"
     CDS             14..1168
                     /locus_tag="PWY98_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2038382.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1162..1584
                     /locus_tag="PWY98_002692"
     CDS             1162..1584
                     /locus_tag="PWY98_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038383.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2718..3686
                     /locus_tag="PWY98_002693"
     CDS             2718..3686
                     /locus_tag="PWY98_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2038384.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3696..4184
                     /locus_tag="PWY98_002694"
     CDS             3696..4184
                     /locus_tag="PWY98_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2038385.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4480..4761
                     /locus_tag="PWY98_002695"
     CDS             4480..4761
                     /locus_tag="PWY98_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038386.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4763..5179
                     /locus_tag="PWY98_002696"
     CDS             4763..5179
                     /locus_tag="PWY98_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038387.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2148 a          983 c          943 g         1696 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga atcttttagg ggtctttttg tctgttttta ggggtctttt
     2581 tgtctgtttt taggggtctt tttgtctgtt tttaggggtc tttttgtctg tttttagggg
     2641 tctttaatta gggggttgac ccccattaga aagctcttta taattttgtt aagtagttaa
     2701 tcttgaagga ggattttatg gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga
     2761 ttcctttacg caaatttacg tcagtagaaa tgaatttatt cttctctata atatctagaa
     2821 tgcgtgatgt aggcgataaa aaagtacaat ttacattcga acaattaaaa gatttaagca
     2881 attataaagc aactgcaaat gttcgattca tagatgattt agaaacaacg tatgacaaat
     2941 taatggattt acgttttggt agaagaagtg ctgacggttt gcaacgtgaa cgatttgttt
     3001 tatttaatca atttaaaatc gatggaaaag ctgatattcc ttttgctgaa atacaagtcc
     3061 atgagaaagc cttaccctta cttaataatc ttgaagaatg ggttcgatat tctctccaac
     3121 aattcaatga attagaaagc agttattcca aaaccatgtt tagattatta aaacaataca
     3181 gaaccaaagg aattgccaat ttttcaaaag aagactttca tgaattactt gatatcccta
     3241 aaagttatag agaaagtgat ataaataaaa aaattataaa gcctattcgc caagaactaa
     3301 ccgcaatctt caaaggtttg acaatccaaa agagatacgg aaaaggacgg ggaaagcctg
     3361 tcattggcta tcaatttaca tttaaacctg agataaaaaa atcagacgac tttaacaaac
     3421 atgctccaac caaagaagaa caaaataaag ctttggaact agctaatcaa caggttcaat
     3481 ctgaaaaaga acctacttca ttacaagaaa aaatcaaaga acaaaaacga aaattagaac
     3541 ttttacaaca gttagcagaa ttagaaagaa aacaaaatac cccaagtaag aaaattaaca
     3601 aacaaatagc agataacaat tccgatatta ccccagaatt taaagaaaac ttactagata
     3661 atctccaaaa cttatttaaa gaataagggg tttttatgac agataaaact atcaaagaat
     3721 tagcagatat tctaggtgtt tcaaaagata aagtgaagta tcaactgagg aaattaccta
     3781 gtaattttac ctacaaaaaa ggaaatatta cctacctgaa aaaagaagca gtaacaacaa
     3841 ttacaaaggt tttatcaggt aatgaatcgg gtaacttacc tagtgaatta cccgattcat
     3901 tacctaccga ttacctactc aaggagattg aaactaagaa taatcatatt gaagaaaaag
     3961 ataggcaaat tgaaaaactt caaatagctt tagaaacgca gcaaaaatta ctcgaccagc
     4021 aacaacaact caccctacaa gccaatcaac aaatcaaaaa acttcacgaa caactccagc
     4081 taacctatgc agaagaatct tcagaaaaag aacccctttc tgttactgaa gaaactcaaa
     4141 aaggacaaaa caaggagaaa aaatggtggc aattctggca ataacccaac aatttttatt
     4201 cataaaaatt gttgggttac tttctttttt tgcttttaca agcatagcga catgatctca
     4261 cgtcgctatt ttttaaagag cataaggaaa cgcggtatca ctttcttacg aaaggtgcta
     4321 ggtaggctgt gcctaccacg tttctaagaa aaaaatttgc tatcactttt tcgtcattgt
     4381 ggatagctta tgtatagtgt ttcggcaaaa aacagctata caaatactat atttgtatga
     4441 tatacataaa ttatacacaa atgcgaaagg aattcttcta tggacatttt agttcggaat
     4501 attgatccaa aagttatttc agaaatagat agacgttgcc aagaactcac aacaaaaact
     4561 ggcaaaaaat ggagccgtaa tgcgtaccta aaagttctaa tcgaaaacga ttttgaccag
     4621 gcacttaggc attacaaaaa agatcaattc gatttattac tcgaaaaatt tatcgatata
     4681 caagcaaaaa atactgaaac attgaacgac tatgttcata caaacaacca actcattgaa
     4741 acacttatcg gaggaatata acatgagaaa aacttctgtt caaaaacacg ttacccttta
     4801 tcccagtcac attgacgttt tagattccat catcgaaaaa aatccagata tcaataacta
     4861 ctcggaagcc attcgttttc tatgtatgag ttatgaacct aatactaata cgacaactac
     4921 cagcaaccaa gcaaaactaa atgcgatggg aaaagaaatt tctattatgt ccgaaatcat
     4981 tgcagaattt gctgatttat atctaaaaga tactggtgtt cttatcggta caaattctaa
     5041 agtatatcaa gaagtgaaag aaaaaattga aaatcaactc aatccctccc gacgaaatca
     5101 acccaaaaat cgtcaaaaag aaaccaatca aacattcact tctagaaatt ttgacccaac
     5161 aaactttcta aaaagatgaa aaagaaaccg aaaatttttt ctatttctca gtttcttttt
     5221 tagggggcag tcttccagca agatttgcaa gttaccggta acttgcacaa aaaaagaaaa
     5281 atccgcttgt gcgaatcctt ctttttgcat tttggggcgc tgccccaatc tccaaatatc
     5341 ggttatttaa tagcgtggta actcaaaaaa atagaacgta ctctgaaata aaaaaagact
     5401 gctctaacga gcagccaata aaaaataatg agcgtttatt cttgaaaaag cagcgcgctt
     5461 atgaagcgct gccaaaaatt taccatattt tatgaaaaat aacaagtttc taaaaaggaa
     5521 tcggttaaga ttcacaaaga atcgtaaaca gacaatagcg gaaaatcaag cttccgaacg
     5581 taccttttag ctagaccaat gaaaaatcac aaagtgagcc cctgtatcgg ttgatacaag
     5641 ggctacttgg catactcgac attatgtcta ctagctcgat aaataacgat tatgttccca
     5701 aaacaatatt ttatgaacta taacaaaaat ttgttatcgc atattgtata ttttaattat
     5761 aggtgtatat
//
LOCUS       DANKAY010000142         5689 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000142 DANKAY010000000
VERSION     DANKAY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(488..1297)
                     /locus_tag="PWY98_002697"
     CDS             complement(488..1297)
                     /locus_tag="PWY98_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2038388.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="PWY98_002698"
     CDS             complement(1297..2043)
                     /locus_tag="PWY98_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2038389.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="PWY98_002699"
     CDS             complement(2061..2528)
                     /locus_tag="PWY98_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2038390.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="PWY98_002700"
     CDS             complement(2528..2938)
                     /locus_tag="PWY98_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2038391.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="PWY98_002701"
     CDS             complement(2949..3962)
                     /locus_tag="PWY98_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2038392.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="PWY98_002702"
     CDS             4179..4913
                     /locus_tag="PWY98_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2038393.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="PWY98_002703"
     CDS             4897..5607
                     /locus_tag="PWY98_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2038394.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DANKAY010000143         5627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000143 DANKAY010000000
VERSION     DANKAY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(94..645)
                     /gene="raiA"
                     /locus_tag="PWY98_002704"
     CDS             complement(94..645)
                     /gene="raiA"
                     /locus_tag="PWY98_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2038395.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(773..1492)
                     /locus_tag="PWY98_002705"
     CDS             complement(773..1492)
                     /locus_tag="PWY98_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2038396.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1489..2802)
                     /locus_tag="PWY98_002706"
     CDS             complement(1489..2802)
                     /locus_tag="PWY98_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2038397.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2883..3203)
                     /locus_tag="PWY98_002707"
     CDS             complement(2883..3203)
                     /locus_tag="PWY98_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2038398.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3311..3598)
                     /locus_tag="PWY98_002708"
     CDS             complement(3311..3598)
                     /locus_tag="PWY98_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038399.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3725..4369
                     /locus_tag="PWY98_002709"
     CDS             3725..4369
                     /locus_tag="PWY98_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2038400.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(4428..5135)
                     /locus_tag="PWY98_002710"
     CDS             complement(4428..5135)
                     /locus_tag="PWY98_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2038401.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5355..5525)
                     /locus_tag="PWY98_002711"
     CDS             complement(5355..5525)
                     /locus_tag="PWY98_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038402.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         1629 a         1181 c          971 g         1846 t
ORIGIN      
        1 catatttcat ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc
       61 tgcaagtttt cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt
      121 tccatcttta cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa
      181 gaagttatgt cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa
      241 tgaaaggcgt ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc
      301 attgaataaa gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat
      361 ttttgttttg aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc
      421 gtataaatct ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac
      481 ttccactttt gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg
      541 tgcgtcactg aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc
      601 ttcagttact tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt
      661 cgtctaccac aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat
      721 cgaactctag gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg
      781 ccaaagaaat ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga
      841 aaagagtctg tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct
      901 tagaaaaggt gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt
      961 tttttgcttg agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg
     1021 ctgctttcaa catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata
     1081 aaggtattgg acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg
     1141 caaaggtgcc tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca
     1201 tattttcatc gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct
     1261 tccattcttg acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct
     1321 ctagattgaa atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa
     1381 attgtgcgcg acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct
     1441 cgctgaatgt taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt
     1501 cttcccttct tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt
     1561 ttcttcatag aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc
     1621 tgcgatttgt accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac
     1681 ggatattctt tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata
     1741 ttttgcttct cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc
     1801 tgtgatctca tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt
     1861 gatacttgga caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac
     1921 caaagatttt atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa
     1981 gacgggttca ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt
     2041 ctgcttgatt tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc
     2101 gtctggttta atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa
     2161 cgcatcgatc tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg
     2221 aatcgtacag ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa
     2281 cgtgattttt tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc
     2341 gactcgcggt gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg
     2401 ataaagcatc tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc
     2461 tgaatggcaa agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca
     2521 aaaaaagacg acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg
     2581 agtgtcgcat cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg
     2641 tccatcaatg acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg
     2701 ttccactgct gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc
     2761 aatatctctc gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa
     2821 atatacgaaa aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct
     2881 atttattcac ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca
     2941 taatcagcca tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa
     3001 tcagctttca taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca
     3061 aggttgttat ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct
     3121 tcagcagctt tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca
     3181 agcatgattg tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt
     3241 tttcttcgcg tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa
     3301 cgcggtttat ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt
     3361 ctttgtccaa attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt
     3421 tttcagggtg tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa
     3481 gtccgttact gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc
     3541 cgccattgtt cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc
     3601 ccagatccac ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca
     3661 gtgttgaaac aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga
     3721 caagatgatc gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa
     3781 aaaatcacgc tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt
     3841 cattcaagaa ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat
     3901 tggagaaaaa aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg
     3961 cgtaccgatg ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac
     4021 tcgatatttt ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc
     4081 ggtctctcaa gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc
     4141 tgtgatgatc acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt
     4201 ttttataaaa gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa
     4261 aagtgctgac cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga
     4321 agagggttct gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat
     4381 tctgattctc ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa
     4441 taaaactcaa aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct
     4501 tcaaaataag aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag
     4561 tcagcaattt tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt
     4621 ttcccgcctt tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag
     4681 ccatcgatca gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg
     4741 aaacgaatac gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt
     4801 tcacttgagc ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt
     4861 tgtgattcca taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct
     4921 cgcgcaacat acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt
     4981 tgaattgctt gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct
     5041 gaaggaagac ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca
     5101 tgaatctgta tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct
     5161 tctattataa ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc
     5221 aacagaggat ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag
     5281 atatgttcaa cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg
     5341 atcttttgtt attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt
     5401 tttgatttta cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat
     5461 ttttctgatt tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat
     5521 ttcatgtgga tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc
     5581 ttcaaggaac aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DANKAY010000144         5592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000144 DANKAY010000000
VERSION     DANKAY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(1014..1304)
                     /locus_tag="PWY98_002712"
     CDS             complement(1014..1304)
                     /locus_tag="PWY98_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038403.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="PWY98_002713"
     CDS             1706..1942
                     /locus_tag="PWY98_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038404.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="PWY98_002714"
     CDS             1949..3901
                     /locus_tag="PWY98_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2038405.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PWY98_002715"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PWY98_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2038406.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="PWY98_002716"
     CDS             complement(4797..5489)
                     /locus_tag="PWY98_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2038407.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1823 a          972 c          903 g         1894 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat ac
//
LOCUS       DANKAY010000145         5584 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000145 DANKAY010000000
VERSION     DANKAY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..443)
                     /locus_tag="PWY98_002717"
     CDS             complement(<1..443)
                     /locus_tag="PWY98_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038408.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKR"
     gene            complement(750..1847)
                     /locus_tag="PWY98_002718"
     CDS             complement(750..1847)
                     /locus_tag="PWY98_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2038409.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1955..3982)
                     /locus_tag="PWY98_002719"
     CDS             complement(1955..3982)
                     /locus_tag="PWY98_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2038410.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4127..4858)
                     /locus_tag="PWY98_002720"
     CDS             complement(4127..4858)
                     /locus_tag="PWY98_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2038411.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            complement(5174..>5584)
                     /locus_tag="PWY98_002721"
     CDS             complement(5174..>5584)
                     /locus_tag="PWY98_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2038412.1"
                     /translation="IAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNY
                     PKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLER
                     FLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         1580 a         1107 c          894 g         2003 t
ORIGIN      
        1 tttctctttt caaaaaaaat ttgatatttt tttagatttc tttttagtct ttgtaatact
       61 ttataagcca cagaataact cacgtgatgc tgtttagcaa aattttctac tgagatatat
      121 ttttcctgaa agaaagccaa taccattttc atagataaag aatccgttat ataacgagat
      181 aacagagctt tgctagaaca ttttccatta atttccagac atatatccaa ttcactgacg
      241 gttactcgca aatttttctc tagatcgaac ttttgtaaat catgtttcag cttatcgatc
      301 gtttttttca agagaaatgt cgatagcgac aactcttctt gaacattcga tattgactct
      361 gataaatttg agcttgcttc taaatattta agtaatgaat aacttctgtg atcttctttg
      421 cttaaaaaaa actcataatt cattagtcat cagctctttc tacgtatatt actttccatg
      481 cattcaccaa ctagctgggg ataaaactga tttcctggat ccgagtcact gttgtcagcg
      541 attcttgttt gtataatata catcgtcctg tctttttttt aacattttgg agagcgtttt
      601 atttggcctt tctcgcgatc aaacctgtta ttagaattat atcatttact agtatgcatt
      661 tttattaata aaatggtata ttttatattg ttataaaaag agcccaacca tcctacaata
      721 ataaaaattt actatttgtt atcttaacct tacttttctt tctcttcttt ttttctcaaa
      781 acaaacaaaa gtatgcataa taaaataaac aataagaaaa gtgaagctac taagatataa
      841 atccaattat tgtctttttt aatcgtaaca tccgtcgtgt tcaattcctt ggctactttt
      901 ccgtctattg taaaatcttt ttcaaatacc cattgattgg tatagttgat cgtagtattt
      961 cctttttttc tcgaaaattt tccattttca ttttcatttc ctaaaacagt cattgtaagg
     1021 tgatattcac ctggggttag cggttgacca tttaaagcag ttggaaaaga aaaatttgta
     1081 ttcggcgcaa tctgcaatcc ttctttttcc tcttgatata aaacttcaga atgtcctttt
     1141 tttgtaattt ttgtttcaat aactacttga ttgatatagg ttttttgatc attttgaacg
     1201 tttgctaaga tgacatttct tgcattgatt tgatctggtt taacttcatg aagagtaagg
     1261 ttcggagcaa cttcgttcag tgtctgttgc atcaacaaag caataatata ggtgtattca
     1321 ttctcaatcg ataagctttg atccttagtt tctgtttggt ccttttccgt ctggatttct
     1381 tggaatgtga tcccaccagc caatacacca tcaaatcgct cattcggcat cctgacatga
     1441 aaagcaacgg tttggacact tttaggtttt agcgtcacta tctgtggata gctcacataa
     1501 tctaccagat caaagatcag gctcatgtct ttttcaatct cgttttttcc gtattcaacc
     1561 atcacatttg aattggtggt tgcgctattc acagaaatat cgattttcac ttctttttct
     1621 gtatcatttc tcaattccgc ttttacttct acttcctgat ccggatcaag ttgaagatcg
     1681 aagtaagttt ttctcttgtc gatttgattc tctgaagtta tcggcgtcac tgaaaaatta
     1741 aatgcactag ctgacactga tatattgtta aaaagtgccc ctaaaataac taggagtgtt
     1801 ataaataatt gttgttttgg tctcaagaaa atcgctcctt taatcatatg taagagaaga
     1861 ctataacaaa atcttgtctc tgtgggacac aagagcctat ggtacctgtg cccttcttga
     1921 aactcaataa ttttgttata ttcttctctt cctgttaatt tcctgggaca tctgcaagta
     1981 tccaagatag tttcccctca aaggttccga ttctttggtt ttctacagga atatttattc
     2041 tgattccacg atgagctgta gaatctaaaa gagaggacaa atcgctttca cccttttgat
     2101 cactttcgaa aatcgtaaca gcggatgtcc ctataaccgt tgtattttca gaggtaacaa
     2161 aggaaagagc ttctggaagc tcaaaatctc catttgtaag aactttctct tgtttcaatt
     2221 gtaatttcca acttccaaaa gcccgttcat ctataatttt caacccacct gaaaactgtc
     2281 cgaattcact taatggtcta ctggatattt tatgttttcc aaaattgaca tattgaggag
     2341 cagttgagag ataaagtttg ccttttctgt aataaaaata gatatcctct gtattttcgg
     2401 agaagatgcc ttctgtattc gctgaatccg agtcaaaaac atacccttca atatcaatag
     2461 gagtttcttt atacttctct cctattttcc cagtgattgt tttcatttca gcaatatcaa
     2521 tctgactgtc tttatcttta taataaacat ggatatctat ctccttcagc tttgcgatct
     2581 ttaggtttat ttcattcgat tgctcaatca aattcccgtt ttcaaggtat tcaaaatgca
     2641 tagtgagtac tttttcagga attacttcct gcaaagttgt ctccaattcg attattaacg
     2701 tatctccagc aataaaataa tctgtcagat ttagcgtgac tcgatttccg gttatcaaga
     2761 tatctttggc atcaatttga tttccattga tcaagatatg ctcgggaata gttaattctt
     2821 gtgggaggtc gatactagtc tgcattttct tatattcaag aaaatccccc tcgaccttca
     2881 atgtatttct gatagttatc ttatcgtttc ctccagcttc agcgatggcc ccataatcac
     2941 tatcgttgac ttttacatca gctgttgatg tgacattcga tggaagtcgc gtgacagcga
     3001 cagcattttc ttgaaaaaaa gtacctgttg aagaagtaaa cccccaattt acttctgtag
     3061 aaccaaactg cctaatgaga tcaggtataa tccgagaaga gcgaccgaca ccttccaaat
     3121 cataagtaag acttctttgc tccgcgtccc atttaattat taacaagcgc catgtgccat
     3181 tagaaagaaa tgtaggagct accgtcacgc cagtatgctc tccagaataa ttattattat
     3241 ttggtttagg cgtgacaaaa gcgacatgtc catgccctct attatcagca gttatctctc
     3301 gatccattcg atcagaagaa ccactatttt tatacgtatc aaactcaatc gagagcgcat
     3361 tcgcaatgta aggttggcca gaacgagtag aatatgagcc aattcccata cctggcgatc
     3421 caataacttg actgggatta ctagccattc taggatcgcc agtcaatgta aaggtcatgc
     3481 catctcccgc tctatctcgt tgatttccta agtataaata agatttaaat gtaaaatccg
     3541 agagtaaact aatcttctta tttgaccaaa tagctcctct ttgagacctt tctgctgggt
     3601 tcaactgcag taacttccca ttttccaata tggacgtgct agtaccgata ggtgtttgaa
     3661 aaattttatc gatagaaata atattgggat cgtcaggcag ctcaaccgca gaaattggtg
     3721 catcttctag tccttgtttg tccgaaccct cagcttctac gttgtcagtg gtctctccac
     3781 tactcgaaat acttaaatcc tcactgcttt cttgtgtctg gttggattct attaaactgt
     3841 cgaatgtcga agtgagtttg ctttcttcgt ctcttctatt tccatgctcc aactgattca
     3901 attccaaaga gtaccctatg atcggaaaga tattagtcac taagagactg cttaaaatga
     3961 gcacgatcct cctctttttc aatatatctt ttcctcctga tcgttaaatt cctagatgaa
     4021 ctattcgtat ccaaacaaat ttagcctagc ctttgacaat ttttgctcga ttacctcgca
     4081 agacaatatg actacgtatt tatttttaac agttcttttc agtaaactag ttgttaggtg
     4141 tatcttctaa tgtccaaact aatttagtgg aatattgttg cgctaacttg acagatttac
     4201 cgggtaccga aagctgtacc gcatcttgat tttcattttc atttgcacca aatcgataga
     4261 cccaagtccc cacacctgtt cctgatttag cattaattgc taacgtattg tttcctcccg
     4321 gtgtcaacgt taattcagaa ttcaccgttt gcggcgtata tgttggatca accaaagaaa
     4381 cagcctgccc tttcgtaaat ttcagctgtg ctccaactag ttcatcccca ctggcagttt
     4441 taaactgttc tggttgagag acacttaatt tccacccaga taaggtactt ctcctatctg
     4501 tcacttggac atatgtcggt tttctagacc catcgttcat cacatcagca gctgcataat
     4561 acgttttatc cgcagtagaa attttttgag tcccaaattt gaattttgat ccataatcaa
     4621 tcgataatgc ccctcctgtt ccagggcttg gaggatctga tggatcgaca ggtttattgg
     4681 gatcaggatt ctctggatca acaggaggag ttacaccctc atctcctgct tcaaatgtaa
     4741 tcgttccttc tgtgttatat tcctttgggt atacttcagt tgcagaaaca aaagtcgtac
     4801 ttgctaccat gctgaataac atcattgctg ctgttaatac gatttctttt ttcatcattg
     4861 acattcctct ttctttattt actaaacata tcagtcgatt ggattaatcc atagcaaaat
     4921 tctgttttac gatacttatg aaataaaaga acatagacct atatttggtc ctatgtatcg
     4981 attagttata aatgattatc agcccgattt gatttctagt ttttattgca acttgatgca
     5041 aacacaatct agaaatacag ctgtcatagc ctttaaaata attcgtaagt ccccctccta
     5101 tctatcaaaa tgtgacttga cgagagtgta aaatattttg tgtaaataaa ttgtcctcct
     5161 gcaaaataat tagttactca gtaaacattg aaactaatgt atcggttacc tgttgaaaac
     5221 ctttatggct tctgtttaga aatttttgat tgtatgtatc aaaaatgctg actagaaagc
     5281 gttctagtga ttcttcattt tgaaactgct cttttctacg gctgtatctt ttaatttgct
     5341 tattgaaaga ctcgattaga ttggttgagt aaatggttct acgaatgcta ggtggaaaat
     5401 cataaaaagt taataagtct tggttttcta tgagtgactg cgtcacttta ggatagtttt
     5461 tcttccattt ctcaatcatg ccggataaga aggtattcgc ttcttctttt gagttagctt
     5521 gataaacagc cttaaagtca tcacagattt cttttcggtc tttgacacgt actttatgag
     5581 cgat
//
LOCUS       DANKAY010000146         5544 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000146 DANKAY010000000
VERSION     DANKAY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            103..2637
                     /gene="secA"
                     /locus_tag="PWY98_002722"
     CDS             103..2637
                     /gene="secA"
                     /locus_tag="PWY98_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2038413.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2763..3864
                     /gene="prfB"
                     /locus_tag="PWY98_002723"
     CDS             join(2763..2834,2836..3864)
                     /gene="prfB"
                     /locus_tag="PWY98_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2038414.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            3969..4655
                     /gene="ftsE"
                     /locus_tag="PWY98_002724"
     CDS             3969..4655
                     /gene="ftsE"
                     /locus_tag="PWY98_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2038415.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4639..5532
                     /locus_tag="PWY98_002725"
     CDS             4639..5532
                     /locus_tag="PWY98_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038416.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
BASE COUNT         1901 a          983 c         1247 g         1413 t
ORIGIN      
        1 tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta aagtctacat
       61 agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa tttgattcgt
      121 tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc aaaaaaagtt
      181 gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc aaaaactgct
      241 gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc ggaagctttt
      301 gcagtcgtcc gagaagcagc aaaacgtgta ttggggttat atccatacca cgtacaattg
      361 atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg tgaaggtaaa
      421 accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg ggtacacgta
      481 gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga actttataat
      541 ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga aaaacgcgag
      601 gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga ctacttgaga
      661 gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta tgccattgtc
      721 gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa
      781 gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg tttgaaagaa
      841 gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga agcagggatc
      901 gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa tactgcattg
      961 acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga tatcgattat
     1021 gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat catggatgga
     1081 cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt agagatcgaa
     1141 gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat gtacaagaaa
     1201 cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga aatttataac
     1261 atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg tccagattta
     1321 ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa agatcgctac
     1381 cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga gttattatca
     1441 gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa tcactttaaa
     1501 gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc taccaacatg
     1561 gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg tggtctagca
     1621 gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg aggacgtgca
     1681 ggacgtcaag gagaccctgg tatgtcacaa ttctacttgt cattggaaga tgatttgatg
     1741 aaacgattcg gttcagaacg aatcaaagct ttcttagatc ggatgaagat cggtgaggaa
     1801 gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca aaaacgtgta
     1861 gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt gatgcgtgaa
     1921 caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa aagcttatcc
     1981 aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc gcatacacaa
     2041 ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag tactcttgtc
     2101 catgaagata gtattactct tgcagatatc gaaggaaaaa catctcagga gatcaaagag
     2161 tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact aaatggtcca
     2221 gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc taaatggaca
     2281 gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc ttatggacag
     2341 aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga tatggtaggc
     2401 gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca aaacgtacag
     2461 cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc caaagaaaat
     2521 agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag aaatgatcct
     2581 tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc aaactaattt
     2641 ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac cttctttctg
     2701 ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa gaaggaaaaa
     2761 acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag gtcacaagtt
     2821 tcaggaggtc tctttgactt agatcagctg gaagaagaca tcgcggaagc agaagcaaaa
     2881 atggctgaac ctggtttttg ggatgacagc gaagccgcac aaaaagtgat cagcgaaaat
     2941 aacgctaata aagaaaccta tgaccaattc cattctcttg ccgaagaact agaagaaatc
     3001 gaattgatgg tggagatgct gcaagaagaa cctgatgcag caatgcaaga agaagcagaa
     3061 acacgtatca atgcattaga tgaaaaaatg aagacgtatg aattatctat gctgttagac
     3121 ggaccttatg atcgaaacaa tgcgatcgtc gaattgcatc caggggctgg cggaacggag
     3181 tcccaagact ggggcagcat gcttttacgt atgtatacac gttgggcaga gcagcatggc
     3241 tatcaagtag aaacactaga ttatcaagca ggtgatgaag cagggatcaa gagtgtgacc
     3301 ctgctgatta aaggacataa cgcttttggt tatctaaaat cagaaaaagg tgttcatcgc
     3361 cttgtacgga tttcgccttt tgactcagcg aaacgacgtc atacttcttt ttgttcggta
     3421 gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga tgatttgaaa
     3481 atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac agaatctgct
     3541 gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca acgctcacaa
     3601 ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca attagaagtg
     3661 gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat cggctggggt
     3721 tcacagatcc gttcttatgt tttccatccc tattctatgg taaaagacca tcggacaaac
     3781 gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt tattgacggc
     3841 tatttaaaaa tgcatctaca ataagaactc attgttaaat tgaaataaaa ttgtaagatt
     3901 ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa agatatggag
     3961 agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt acgaccgcta
     4021 tccgcaatct ctcgattgtc atcgaacaag gagagtttgt ctatgtggtt ggtccgtctg
     4081 gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa
     4141 ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt ccttatctac
     4201 gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa acagtatatg
     4261 aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgagatatc aaaaaacgag
     4321 taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt cctagcgaat
     4381 tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatcgtgaat acgcctaaag
     4441 tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg gaaatcatga
     4501 aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca cataacagta
     4561 cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc attattcgag
     4621 atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca tttattagaa
     4681 agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag tgcggtaacg
     4741 atcacgttga ctttagtcgg tgtattcatg gcagttatca tgaatgcaac taaattagct
     4801 caggatatag aaggaaacgt tgatgtatct gtattcgtag atatcggtac aaaagacgaa
     4861 gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa agttgaattt
     4921 tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc ctggaaattg
     4981 tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac tgatccttct
     5041 tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg ttctgattac
     5101 ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac atggggatta
     5161 gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac gattcggatc
     5221 acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc aaaaaatgga
     5281 tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg tgcgattctt
     5341 ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa tccatcatta
     5401 ttacgttccc attattcatt gattcgtcca caaaatatcg tttggaaaat caatctttta
     5461 atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat gagacgtttt
     5521 ttgaaattct aataccacaa tata
//
LOCUS       DANKAY010000147         5528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000147 DANKAY010000000
VERSION     DANKAY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1006
                     /locus_tag="PWY98_002726"
     CDS             <1..1006
                     /locus_tag="PWY98_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038417.1"
                     /translation="NHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQR
                     MKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMF
                     GVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHL
                     YSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRL
                     IFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPD
                     IVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN
                     "
     gene            complement(1032..1472)
                     /locus_tag="PWY98_002727"
     CDS             complement(1032..1472)
                     /locus_tag="PWY98_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2038418.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1632..2138
                     /locus_tag="PWY98_002728"
     CDS             1632..2138
                     /locus_tag="PWY98_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2038419.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2306..4219
                     /locus_tag="PWY98_002729"
     CDS             2306..4219
                     /locus_tag="PWY98_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2038420.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4224..5225
                     /locus_tag="PWY98_002730"
     CDS             4224..5225
                     /locus_tag="PWY98_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2038421.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5427..>5528)
                     /locus_tag="PWY98_002731"
     CDS             complement(5427..>5528)
                     /locus_tag="PWY98_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2038422.1"
                     /translation="EPAFFCLPLGFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2023 a          850 c         1119 g         1536 t
ORIGIN      
        1 aaatcattct gatatattat caagtataaa aaataaacgt caactattag aaaaatttgc
       61 gaaacatgcg ccatatacgc gtagtataga tagaaagaaa tggatcagcc tactaagaat
      121 ttcgttgcaa cgaatgaaac aaggccactc gattacgtat gtcccagaag agatgaacaa
      181 atttgtgcat ccattgataa caaaagaaga gtttacgcat tacatactag agcctttttt
      241 tgaagcaaac ttctggtcga tgaatcacat cactccaaca gagcaagctt acctttattt
      301 tatgtttggt gtaatgaaca cctatttgga agaagatttg ttggatactc cagataaagt
      361 acaagaaagt tggggctgga ttgaatcgtc tattgtagaa aattttgaaa aaaacttcaa
      421 gatttgtttt acaacttcag aaaaaaatta tctatatgtc aatttggcaa tgatccattt
      481 gtattcaagg gtttttggga caaaaaagaa ggtggatgct tttggaaaat cagcagaaga
      541 cagagatttt cagcatatat ttccagtgat gtatccaatc atgcaacagt tttttagtgg
      601 ggtagctcgc agaattccag agcttaatac ttattatgaa aagaacagac ggttgatttt
      661 tcaatactgt atgttagtca gggatatttt tataaaacac gaacaacctg tcatattcta
      721 tattcaatca aagtatggta aaacacagga attatgggca aagaaaagga tattatctat
      781 gactgaacgc ccaataaaat atagtgcatc cgctgatcag ttacctgata ttgtcattag
      841 cgattatcct atcgacaaaa aggcatatga tcaaacgaat acacagatat tttactggaa
      901 tggacaacct acaaaaaatg attgggcgcg tctgaaagct gaaatcataa aaatcaggaa
      961 tgagaaaaat agatccatat tggaaaaatt ggatttcgtt aattaagaaa agcagattgt
     1021 ttatgccaaa attaagacat tgcaaaaaga ttattttctt taaaatcgac aagatgacca
     1081 gaaagcaaga gttcgttgag ctcttgcttt cttttagttt tagaaaggaa catcctgatt
     1141 ttatcttcgc taaatccagt caaaagtttt gcagattggt cgtagataat cggttttttc
     1201 aataaagaag gggatgtacg aatctcaata tatagttcac tcaaactgag ttcatctatt
     1261 ctgtgaacaa gatttttata actaatagaa tgagtagaga gcatttcggt cacgccgttg
     1321 tttgtcaatt ttagtaagtg gataaattca tttttggtta gtggttgacg aattaagttc
     1381 tgttcgataa acggtatatc atggacgata agccaacgtt ttgcgcgtct tgaagacgag
     1441 cagccagaac ctgtatacaa tttaatcatc atacattcct ccaatagcta agtatttatg
     1501 aaataataac atgaagaaat agtcgaaaag tgccatttag aggtgtaaaa taagaaaatg
     1561 ttgtcacgta taatggaact agctagataa gaaatactat taaatcaaga aaaaagaaag
     1621 aaggcacaag tatgtcaaac acagccgacg caatagtagt gatcgattta caaaaagggg
     1681 tatgttttga ttcttcgccg atacacgaat tagaaacagt gacagcatta gtgaataaaa
     1741 gaatcaaaac atacgaaacg attgggaaac cagttatttt cgttcaacac gaagatgctc
     1801 aattagtgcc acaaacaaaa ccatgggagc tattgtcgga attatctatt cctgagcaca
     1861 gttattttgt gagaaaaacc catgcgaatt cattttataa gactaatttg agtgttttat
     1921 taactgaatt ggactgtcat tcgatcgaat tatgtggggc acaagcagag tattgtatag
     1981 atacgactgt gaagtttgct catggattag gataccagct tatgatgtgt agaggagcaa
     2041 cgacaactta tgataatcct tttatgaaag caaaggaaac aatcgctttt tacgagaaaa
     2101 tttgggataa acgatttctg acatttttgg agcaataagt tgtttcttcg tttaaaaaaa
     2161 gcagaatgga tactggttta tccattctgc tttattacgt aaaatgagaa aataatcctg
     2221 ttctattttt atgggataaa ccatttattt tttgtataaa aaatgtttgc gtttacatat
     2281 aattctccta gaaacataaa aaggagtgaa tagcttgaga acaaaacaag tattaaaggg
     2341 agcgttggtc atcttatttg ctgtaagtac aagcgtcccg caatggacag tatatggaga
     2401 gagcatttct tccgaatcgg aatctcggga ggtagaaacc acagaaacta ctgatgctgg
     2461 aaatgaaacg aaatcgagta cttcacaaac agaaacaaat actgaaacaa cggttgagac
     2521 atctgttact agcacagatc aagaaacgac cgctaccaca gataaccaac aaacagaaga
     2581 agcaacagct acaaaaatgt caaacgacga atcctcttct gctaaagtaa gtgacaaaga
     2641 gaatgggaca gaaaatgatg atatcggtaa aggtgaaaat ttcaatggta aagaatattt
     2701 gaattacttc ggtaaacaga tgacagaaat acttaatgga ccaacagaag acatcaagtc
     2761 aaaattaata gagttattag tcactgagct tgctgttaat gattatggtg tagataaaga
     2821 gattatcaaa catctgattg ctacttatct attgtacgat atcgcttttg caaaaaacag
     2881 tgtgttgatg tcgatgcttg gaggtacaga tgatgggaca acgcctacag aaaacacagg
     2941 cttctatcca aatacaaaac aacttcatca aactgtacta aacaaacaag atggcaaaat
     3001 gatggatgta tatgcatatt atgtcgatca aggttcagat aaaacagtca tgatccatgg
     3061 cggatttcga gggaattgga ataacgggat cgttacagaa gaatacaatg atttttataa
     3121 ggccgggtat aatttattat ttgttgattc cagagcgact ggtaatagcg gaggcgatta
     3181 tgtgacctat ggacaatatg agtcagatga cgtcctttac tggataaatc aagaagttcg
     3241 agagcggcca tctcaaaaga ttttacttta tggtggttcg atgggagcag ctacgatgat
     3301 gtctgtatta gcgaaagata ttcctgtcaa tgtcaaagga attattgaaa attgcggatt
     3361 tgcatcaatt gatgaacaac tacgttttac gtacagccag acagtagccc ccgcactacc
     3421 ggatgccata aaaaatcagt tagacatcat tggggatcaa gagcatgaag atttatttat
     3481 gggccttttg aaacaatact attttgatca ggagatgcat ttggatacaa aagctgctct
     3541 acctacgatt ggaatgtcag attcattgcc taaattgatt atccatggaa cagcagatga
     3601 tgtagtacct gtgtctaacg cgcaaaaatt atatgagtta tcaggagggt ataaagatct
     3661 tttattagta gaaggagcag ggcatggaaa ggcacaggaa gttgaccatg ctgcctacac
     3721 gaaacatgta acggactttt tgaaagtagt gttccatgat cagataaacg taaagtatgt
     3781 ggatgaaaac aataaaagtc tactcaaaga ccaggacgaa atccagcttt acggagctta
     3841 tggagaaaat tatatgacag agcaaaagac attcgaagga tacgaattag caaatgttga
     3901 aggaccgact gaagggattt ttaatgagac gataccaact attattttct attacaaaaa
     3961 aatcccagta gctccaccaa aaaagcaaga tccgacagag aatgcagaca ataagggaaa
     4021 gaatgaacct cgagaggatc aatcaagtga tgatacgcaa ccgattcaga tgacagtgaa
     4081 agtacaaaaa aagacagatg caaaaaaaag aatgttgccg aaaactggag aaaagaaaca
     4141 ttcgcttctc atttattcag gtggattttt atctgttagc acgattatca catggatttg
     4201 gcgaagaaag agagggtaaa tgaatgaaat ggttcaagcg aatagtgatt gcagtaccag
     4261 ttatcatcgt agttatttta gtcactgtat ttttgattaa tcaaatcaca ccaaagccga
     4321 ttagcttgct tgttagaaaa caatttgaca caagtgaaaa agaacaagta tatgcccgac
     4381 cagatgattt ccagcaaaaa atcgaacaaa tcgatataaa aaaagaaata tcctatcctt
     4441 caaagtacaa aaataataat atggatatct acacaccgaa agtggaaaag aaaaaattac
     4501 cgattctttt ctggatgcat ggaggcgcat atgtaggagg ggataaaaat gattgtcgtg
     4561 attatttgga atatctatgt tcagacacgc agcaaatcat cgtaaatata gattatgaac
     4621 gatcaccgga agcaaaacat cctacaccag ttatccaatt gaacgaagcc attcaagcag
     4681 ctaaaaaaga gataaaagat caagcagatt ggtcgaatat ttcgattggt ggaaattcgg
     4741 caggtgcaca aattagtgga gaatatttgt tagcacttca aaatgaagaa attaaaaaag
     4801 ctgatcatct tgatccgact ttggagaaca agcagattac gaagtttatt tctttgtctg
     4861 gtctgttgga tccttctgca cttactcaag taagtgataa aatctctagc tttctttatg
     4921 aaaaatgtgg atgggcttac tttgataata aggactttga acaatcagag aaggtcaaaa
     4981 gtttagcctt ggtcagacat gcggatcgtt ggacgcaaaa cacattctta acggatggaa
     5041 atacaaatac gtttacgaaa caaatggaag aagtaacttc tgtatttgag aaagcggggg
     5101 tgaaagtcac aaaagtagat tatgcggaga aaaatgctgt tttgaaccat gaatatcagt
     5161 ttgatttcag caataaacaa gcgcaagaaa catatcaaaa gttagtaatg tttttcaagg
     5221 agtaaagtct tatgatgcaa aagaggttgt aatctgtcac ttagctccaa gtcacaagga
     5281 gcaatttcaa taaacaagct tttcatggtt tatgaatatt gtaacttgtg actgaggagt
     5341 tagagatggc aaggggcacc ttttattcga aatttgggaa tatcacacga ttattgtcac
     5401 gatctctttt acgtaaattt tataagtcag atttgttgtt ttgatcgttt aaaaaaagca
     5461 acaaatccga agcctgcgga ggcaagaagg gacaaaaagc cgagtggtaa gcaaaagaat
     5521 gctggttc
//
LOCUS       DANKAY010000148         5521 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000148 DANKAY010000000
VERSION     DANKAY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..108)
                     /locus_tag="PWY98_002732"
     CDS             complement(<1..108)
                     /locus_tag="PWY98_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038423.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYR"
     gene            complement(111..467)
                     /locus_tag="PWY98_002733"
     CDS             complement(111..467)
                     /locus_tag="PWY98_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038424.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(765..1739)
                     /gene="sgrA"
                     /locus_tag="PWY98_002734"
     CDS             complement(765..1739)
                     /gene="sgrA"
                     /locus_tag="PWY98_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2038425.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1935..2774)
                     /locus_tag="PWY98_002735"
     CDS             complement(1935..2774)
                     /locus_tag="PWY98_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2038426.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2959..3846
                     /locus_tag="PWY98_002736"
     CDS             2959..3846
                     /locus_tag="PWY98_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2038427.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4440..5135)
                     /locus_tag="PWY98_002737"
     CDS             complement(4440..5135)
                     /locus_tag="PWY98_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL2038428.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(5119..5517)
                     /locus_tag="PWY98_002738"
     CDS             complement(5119..5517)
                     /locus_tag="PWY98_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2038429.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1760 a         1105 c          806 g         1850 t
ORIGIN      
        1 acggtattct tctccttcta tcggattacc ttttttcaag ctgcaagata aatgatagtt
       61 tttcaaaata gtattaagtt ctcttaattt tctttgaatc gtacgcaaac ttatataatt
      121 ttttgactaa gattttctaa ggattgataa gtacctttgt aaagtgaatc cagtaaaata
      181 taaccaatcg ttccctctaa aagtatagaa taaactttac tcagtaaaaa ctgagaagaa
      241 aactcgactt ttatcatctt gttttctgaa agaaaagata atttatattc tggatacctt
      301 tcgcaaaacg attctgcttg ttgaatcacc gaattcatct tagccagggt gtagtttaga
      361 tctaaagcga tatccttcac cacatataat ttctcgtctc tttcataaca atagttcaaa
      421 aattgatata cttgcctttc attgatatca ataaattctt caaacaaagt acatactcct
      481 ttttatctat tatttagact tattccgaca gctacatgaa taacgtacaa atagattcgt
      541 tactgatttt tcagagatac taccatgcgc agtgtgcatt gtcaattatt cttgctctta
      601 ttttccattt gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa caaaaataga
      661 tctggcaaag ctacaacttc gccaggcaat acagcactct cgaatgaaat attcactcaa
      721 aaatctatcg ttttattcaa ttttagatct gttgctttta ttctttattt cactcgatgg
      781 aagagagaac gaaaatagct tgctcctaca atcaaaatga gtaacacacc catccatgta
      841 attagcttgt tcaaggttct actaccagtt tctggtaaat cgctttttct attatctgtt
      901 tctttcgctt tttcttgatg atcaattcca cgagaaacaa aatcaggctg ttttagcaca
      961 ctttttaggt tgctccctgc atttctcttt tgttccttag ttggtatgat aggtaccaaa
     1021 tcaagttcat tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc
     1081 tcactactca tgcttcctgt gctactttca ctactcgtac tctccgtact gctttcgctg
     1141 ctcgtgcttc ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg
     1201 ttttctgtgc tactttcact gctcgtactc tctgtactgc tttcaccgct cgtgttttct
     1261 gtactgcttt cactacttgt gctatccgta ctgcttggcg gtccaacaac tgtccaactg
     1321 cttggtttca aattctccgt gatcgtcatc tgtccatcat aaaaaacaat caaatcaact
     1381 ttatctttac tcttgatctc atcaatcgat agttctgcag aaatcattat tgtatactca
     1441 ataccagcgg tccattgtac gtcttttaaa taaacttccc caataacttt ttctgaactt
     1501 ccattaccat cccaaccata tgttagaaca gcatgtacat cactagcatt cacgcttttg
     1561 tttggtttga ctggaatagt aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt
     1621 ttcacagtaa atgaaagatt ctcatttgcc cgttcatttg cagaagtact gattccaatc
     1681 attgctgcaa ttatactcag cccaagaata acaaataata cggtcgctgt tttcttcatt
     1741 tctatcactc tccatataat taccttctat ttgataaagg aattatataa aatgaaaatg
     1801 atgagaacac gacagtcagt tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat
     1861 ttgtttactt tttcgtttaa tcaatgacag acttaaaaaa gcaagaccga tatcagcctt
     1921 gctttcttca tcttttattg ataaacagca gtgtatttct tcatccgctt tgttttatat
     1981 gcatagagta acaaaccaac aatcaagact aacaagaata atgtagttga tatagatgct
     2041 tcagccccaa agtttccccc agtaattatt gtattttgcg attggatcag agtggagaaa
     2101 aaaccttgtt cttgcgttcc gctgactggt atacccaaaa tagctccttg tgtccaattc
     2161 caagtaatat gagcaccaat agccgtaaat aaacttcctg tactgtagat aagtgcgctg
     2221 aaaaagcaac ctgctaaaaa gatattgaaa actgccagaa atgtcatgtt aggattcaat
     2281 ccgtgcaaaa gagcaaataa tgcagcagat ccaatcattg ctattaacgg accaaatcta
     2341 agcatctgat gctgaaaaaa gccacggaat aaaagctctt ctgatgcccc cactgcaata
     2401 aataaaataa aactcgttat cagcggcaca atgtttgcgt ttcctgaatc taaccgatac
     2461 gcccccgtca aatataatgg actagtaata agcagaaaca ctccaattcc aacaacgaga
     2521 cctcccgcta aataccccca gtcttttcct cgaacagaaa caccataatc ttctattctt
     2581 tttccttcga ccttactagc aagaaataaa gctagtaaaa aaacagctaa atgtccgacc
     2641 gtaccggcaa ttgctccaat ccattcacta tttccaataa acatatccgc aagtgcatta
     2701 ttgacaagaa ttcctaaaac aaaaccaact aagactatta aaacagatcc aatcaaccga
     2761 ataatcctga tcatctttca tccctccttt atattaagtt taacaaccct tttctttctt
     2821 gtctcgtgaa aactatgtaa atgttctcca tcatagagag aatagatttt tacgaactaa
     2881 gaatatctac agcaacctta cgaaaatgaa agactgtctc cctaattctt ctgttagact
     2941 gtttttaata aggaaaggat gggaaatttt ttgaaaaaac aactactaat tatgtgcctt
     3001 tcagcatgtg caattttact attctcaggt tgtagcaagt ccaatgaaac gattgcaacg
     3061 tttaaaggtg gaacacttac agttcaggat ttttatgata acccaaaagt taaatcaaat
     3121 aatcaaacac tattaaaaaa aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa
     3181 gagatttctt caaaggaagt aacaaaagaa tataatgaac aaatcaaaaa actcggtcca
     3241 aactacaagg aacaattaaa agcagttggc caaacagaag agacatataa gaaactcttt
     3301 aaacagatgc tggctttcca atacggtttg aaagccaatg taaaattaac agataaagat
     3361 ctagacactg cttggaaaga attctatcca gaagttagta cacagatcat tctattttcg
     3421 actaaagaag aagcagataa agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag
     3481 cttgttcaag cttatggaaa aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt
     3541 gattcaacca atccagagat tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac
     3601 ggggaagttt ctgatatcat tcctgtaaca gatccaacga cttatcagca aagctattat
     3661 ctagtaaaaa tggtaaaaaa acaggataaa ggttctaata aagacaaata caaatctgag
     3721 ctagaaaaaa ttgctaccga agcaaaaact cttgatacgg aattcatgga taaaacaatc
     3781 cgtaaagtga tgaaaaaaga caatgtaact attaaagatc cttatgtaaa aaatatattc
     3841 aaataaacaa atttgaccca attggggatg tggcaaaatt tgagaagtga tttttggaaa
     3901 ttccttctta tttttgaggc aaaccacttt tgccacaact tcatcggggc ctttttttat
     3961 ttgtaccaaa acaaatcact ggatttgtta caatcatgag gatataattt aaaaaagttt
     4021 tttatttcta tcacatgaat caatgcgcca ttaagtagat tctttcatgc ttccctattt
     4081 tttcacttgc cgtatcgctt caaaaacaac tatcataaac tcaaactcga ctgaaaagtt
     4141 attttaaaaa agactaaaaa tatcccttct ggacttgttc ttcttattct gttatactag
     4201 agaaacacga aagatatttt tatgcgacac ttttcatttg tttttatgaa atttattcaa
     4261 aactggcgta acttcagagt tacaaggatg tattagaggt tagggaatac atcgtttaaa
     4321 tgagaaataa gtaatagtta gtaatagtca cccaaacttt aaattaatga atattgaatc
     4381 gtcagatagc ctcctcgagt tttcgagaag gctatcgttt attttatgga gaaattgatt
     4441 cagaagtaat cagcgacctt ttttagttca ccaatcgttt ctgcttggaa agtttgcacc
     4501 caatcttctt ggaagcccct cactgcttta tcaatcgagg gcatctctaa tacttgatct
     4561 agtagctctt tacctaacgt tatcgcatga gctccagatc gaatggctgt agtgacttgt
     4621 tcacaatttt tgaaactagc tgctaatatt tttgttggtc tttgcgtacg ttcgatctca
     4681 gtccgcaacg aatcaatcac tttcatagca tcaatgcctt gattttgcat cctgttataa
     4741 taaggtgcaa tataatctgc tccattatta atagctaaat acccttgtaa ttctgtgtaa
     4801 atagctgtac cggtaatcaa ataaccttct tttttcaact ttttgatcgc ttgaaggcct
     4861 gcttgattca ccggcacctt gataaacgta tcttttccca gttcttctat gataactcga
     4921 gcatcctcta tcatttcttc ttccgttctt cctattgtct gaatatggat aggaatctgt
     4981 ccgattattt ttttgatttc tttcatatgc gagaagaagt ttgacgctcc ttcttttttc
     5041 agaattgtag gatttgttgt tacgcctgtt aaattcaaaa actgttgaca agaataaata
     5101 ctttttgtat ttgctgtatc aagcatgatt tccatatagc tttcccccat tctgatcctc
     5161 ttgatcgatt ttaatgattg tcccctttct taaatgcggc atttccttat cttctacata
     5221 cattgttcct ggaagttccg gctcatgaga tccatcaaat tttatcgtga tatgtcctaa
     5281 ttcattcaaa ttagtttgga caacatctcc tactgctgtg attacataca tttgtttatc
     5341 aaaacagagc ttcatatcac aagttatttc ttttcttacc tgattcaatt caatcttata
     5401 acaataatct gttaatgttt ccggtgcttc atctccaaat aaaatcatta ttttttcttt
     5461 cctgaatact tctgcttcta taccaacatc tgttacaacc gtttcaaaga tcgtcatctc
     5521 t
//
LOCUS       DANKAY010000149         5438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000149 DANKAY010000000
VERSION     DANKAY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            131..505
                     /locus_tag="PWY98_002739"
     CDS             131..505
                     /locus_tag="PWY98_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2038430.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWY98_002740"
     CDS             complement(619..1248)
                     /locus_tag="PWY98_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2038431.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWY98_002741"
     tRNA            complement(1382..1454)
                     /locus_tag="PWY98_002741"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PWY98_002742"
     tRNA            1668..1741
                     /locus_tag="PWY98_002742"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PWY98_002743"
     CDS             complement(1879..2043)
                     /locus_tag="PWY98_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038432.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PWY98_002744"
     CDS             2338..3666
                     /locus_tag="PWY98_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2038433.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PWY98_002745"
     CDS             3868..4701
                     /locus_tag="PWY98_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2038434.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..>5438
                     /locus_tag="PWY98_002746"
     CDS             4717..>5438
                     /locus_tag="PWY98_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2038435.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANI"
BASE COUNT         1831 a          839 c         1073 g         1695 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattat
//
LOCUS       DANKAY010000150         5401 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000150 DANKAY010000000
VERSION     DANKAY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..582
                     /locus_tag="PWY98_002747"
     CDS             <1..582
                     /locus_tag="PWY98_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2038436.1"
                     /translation="RLENKLIYGFFDDKQNKMQDLKYLGKINELNSWADSKKKIYKFI
                     VAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIG
                     EHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCM
                     IGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            572..1714
                     /locus_tag="PWY98_002748"
     CDS             572..1714
                     /locus_tag="PWY98_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2038437.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1727..2704
                     /locus_tag="PWY98_002749"
     CDS             1727..2704
                     /locus_tag="PWY98_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2038438.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            2704..3612
                     /locus_tag="PWY98_002750"
     CDS             2704..3612
                     /locus_tag="PWY98_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HDL2038439.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            3613..4365
                     /locus_tag="PWY98_002751"
     CDS             3613..4365
                     /locus_tag="PWY98_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2038440.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            4370..5317
                     /locus_tag="PWY98_002752"
     CDS             4370..5317
                     /locus_tag="PWY98_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2038441.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         1950 a          677 c         1041 g         1733 t
ORIGIN      
        1 agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa aatgcaagat
       61 ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa aaaaaaaatc
      121 tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat aaaaaaattg
      181 aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt ggcaaaagaa
      241 atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc agatgctgta
      301 attggagagc atgctataat aaacacaggt agcattgttg agcatgattg tagaattaat
      361 aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga
      421 actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc ttggtgtatg
      481 ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc ttatggcaat
      541 ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat tatcaagtcc
      601 tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata attggatagc
      661 gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata ctggttctaa
      721 gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct taagactgct
      781 taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag ctacagtaaa
      841 tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta atacatggaa
      901 tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca accaattacc
      961 taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac gaattttagc
     1021 tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt taggttctac
     1081 ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat ttaacggtaa
     1141 taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa aatatgttga
     1201 gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc aacatagcga
     1261 aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg gacaaatgga
     1321 agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata aggaagcttt
     1381 ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc caaatcgttg
     1441 gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta acgtaataaa
     1501 taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac atttgcaacc
     1561 agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt cagaaaagtt
     1621 atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg aacaagaatt
     1681 tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga acataggaat
     1741 tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac actttaatga
     1801 tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg aaaaggaatt
     1861 tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt
     1921 ttttgtttgt acatccccta taacccatga atttattata aaagaggctt taaatttaga
     1981 ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa taatcgaagt
     2041 agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga gaaaagaaat
     2101 tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat accatgtagg
     2161 acagtattta cccgactggc atccttggga agattatcgc aatttttttg tttcaaacaa
     2221 aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta tagatacatt
     2281 tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg atattgatta
     2341 ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa ctttaagtgt
     2401 agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa agattttaat
     2461 agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt ctagtatgac
     2521 agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata ctattattga
     2581 agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc
     2641 ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca ttgaggtaaa
     2701 ttaatgatga aagtaatttt ttttgggtta gggtcaattg gaaagagaca tctaaaaaat
     2761 ctaataagct atggaaaaaa gaattctatt aattttgata taacagctta tagtagcagt
     2821 ggaaaaaaag agtttgaaga aataacgtat attcaaaata gagacgattt ggatacacat
     2881 tatgatatag cttttattac aaatccgact tttaagcatc tggaaacact aaatgtaatt
     2941 gttgaaaaag ttgattacat atttttagaa aaacctgctt tttctgagag tattgatctt
     3001 actcaatata attatgatta caaaaatatt tatgttgctg ctccactgag gtataaaaat
     3061 atcatggaag ttgttcgtaa tagtattcaa agtaaaaaag tatattctgt aagagctatt
     3121 tgttctactt atcttcctga ttggagaaaa ggtactgatt atagaaataa ttattctgcc
     3181 tttaaatcta tggggggagg agtagaatta gactgtatac atgaattgga ttatattata
     3241 aacctatttg gtttccctaa atcagtaaaa aaacagtttg gtaaatattc gaatcttgaa
     3301 attaacagta atgatttagc aacatatata ttgagatacg atgataaaat tatagaaatt
     3361 catttagatt attttggaag agttcctaca agaaaatttc aattgtttac tttagatgaa
     3421 tgtatcaatg ttgacttatt aaataataaa ataagttttg aagaaaacat tgttaaagaa
     3481 atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta
     3541 gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct
     3601 gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt
     3661 aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg
     3721 atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac
     3781 agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt
     3841 aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga ttcattctta
     3901 caactagaaa gagaagataa tatatttgat ttaattatag atttagattt gacttcacct
     3961 ttacgtgaag tttctaatct tgaagaatta atagatgaac atctaaaaaa tttagataaa
     4021 gacgtctact tttcagttgt ttctgcaaga cgtaatccct attttaatat ggtaaaaaga
     4081 attgaaaatg aagttactat agttaataaa tcaagataca catctaggca acagacacct
     4141 gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt
     4201 aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct aattttagat
     4261 attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt tttagaagat
     4321 tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga tgagctgtta
     4381 tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt taggagaaga
     4441 acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc ttgatatgac
     4501 gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg aagagatcat
     4561 gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg tggcagattc
     4621 agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa tgattttaga
     4681 attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt cagcggctgt
     4741 ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc
     4801 ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc atttatatga
     4861 tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa atccgaattc
     4921 tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa tcgtaggaga
     4981 agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg tttatataga
     5041 tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt
     5101 caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa tcaatgaaat
     5161 tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata tccatgactc
     5221 tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg gtattttaag
     5281 tggaatgact aattatttga aaactgaaat caattaaaac atactaagat aagattagag
     5341 ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca aatctgggat
     5401 a
//
LOCUS       DANKAY010000151         5294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000151 DANKAY010000000
VERSION     DANKAY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(489..863)
                     /locus_tag="PWY98_002753"
     CDS             complement(489..863)
                     /locus_tag="PWY98_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2038442.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..2286)
                     /locus_tag="PWY98_002754"
     CDS             complement(886..2286)
                     /locus_tag="PWY98_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL2038443.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2290..2700)
                     /locus_tag="PWY98_002755"
     CDS             complement(2290..2700)
                     /locus_tag="PWY98_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2038444.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2718..3209)
                     /locus_tag="PWY98_002756"
     CDS             complement(2718..3209)
                     /locus_tag="PWY98_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2038445.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3219..4010)
                     /locus_tag="PWY98_002757"
     CDS             complement(3219..4010)
                     /locus_tag="PWY98_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2038446.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4003..4776)
                     /locus_tag="PWY98_002758"
     CDS             complement(4003..4776)
                     /locus_tag="PWY98_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2038447.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4932..>5294)
                     /locus_tag="PWY98_002759"
     CDS             complement(4932..>5294)
                     /locus_tag="PWY98_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2038448.1"
                     /translation="LNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKI
                     PSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIE
                     IPKGEYELIYTIFEQTIF"
BASE COUNT         1686 a         1064 c          705 g         1839 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatcttcga ttttaggaac aattactctt tctttttttc caaattcttt
     1321 ctctaactta aaacttgaaa atggagtgat ttctttaaaa ttctcaatta ttttttcagg
     1381 cagtttaaca ccaagagttt tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc
     1441 gccccaatcc cagttatttt gatcgttctc cccttctttt gttgccaccc agccagttag
     1501 aaaacggcca gtttcattag ttccgactgt tcttgctgca taataggctt ttccgtcgaa
     1561 agcttcgtct actggtgatt tccaatctga aaataatcct gctccgactc gatatttagt
     1621 ctttttatct tcacaatact ctgaaaaaag taaataccac gttccatcaa tttcaaatat
     1681 atcaggcatt tcaatcatgt taaattcatt attagcccag tagtctcctt tgaaattcca
     1741 atcttttaaa tcttcggatt caaaatagac caatctcccc gtcggctttt tcttacttgc
     1801 tggttttcta gctcctagta tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa
     1861 aggatcgcgc caatcattct tatcatatcc ttcttgagga acaagttttg ctgcttcgcc
     1921 atcctttttc caatgaatta aatctttaga acttgcaatc attaacactt ctgaagtttt
     1981 tccatccaac ttagctttgc gattatgtcc agtatacaaa gctaaatact cattgattcc
     2041 ttggacaata cttccagcgt aaatgtattg atcaatgtca tcgttgccac cacgatgaat
     2101 tacttccccg tattcttcaa atttcacaaa atcagtggtt gtaactagtg accacccaaa
     2161 aggatcagtt aaaggtttag gatttctcgt atctcgttga tgaaatagat aaaaagttcc
     2221 atctttataa aaaggcatgc aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt
     2281 ttgcatttat cactcatcct cttcgttagt atgtctttgc atatctgcaa gacacgcaat
     2341 tgtattcata tttcttagat tttcgatgat atcatcgctt ttttttcctt gtaatctcaa
     2401 attgacagcc tcaatcaaaa ttcctaaatt catgccacca agaagttgaa acccattttg
     2461 aaatacttca cttcttttag caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa
     2521 taccataact tcttgatttt ttacttgaga aactattttt tcaatgtttt cacggaattc
     2581 ttcaatgcct gcatctccaa gtgttaccac atgacaattt aaattatcgc caaaaaacat
     2641 ttttaatgta tctacaaatc caatggcaat atttccatga gatgtaatta taaactccat
     2701 ccactcatcc tctgttctta ataaataaaa tcctttaaaa attcttttga atctctagga
     2761 gtttgtatca ctgtcacttc aactccatca ctaattagtt ctttaaaagc ctctctctct
     2821 tcttcagtaa tatttatcgt cggacggatg cgttctgttc cattccttct tgataaattt
     2881 cctacgttta cacttgttat tggtaaaccg ttatcaatca aaaatttaac atcgattgga
     2941 gaaactgcaa caataagaac tttttgtcct tcatatttac cagaaagaat atttttaact
     3001 gctacttccc ttgtaattat tgaagttcta acattaggtg gtgcagcgag gcgaactaca
     3061 gcttttttta cgtcatcatt ggctacttca ttattaataa ccataattct gttagcactc
     3121 aattgatttg tccattgtgt tgctacttgt ccatgaatca ttcgatcatc aatacgaaca
     3181 tgtataatac ctttcatttt ttcatccata ttcaacacct acttcaaaat atttaacgct
     3241 cttaacgcaa tagaaactac taagattaat agaattacat aaactggttt aacctttcgt
     3301 cctaacaaat aatacacaat tgctactaca catgcaggta ataaaccagg cataacttga
     3361 tcaaagattt catttccggt cattgttaag tcgccttgtt taaattcaaa gccaacattc
     3421 acttttacaa ctgtggcaat gagtgcaccg accaccatta accctaaaat tgatactgca
     3481 tcagtaatag actttaattt tgtttcaatt tgagttagta ggctcgcacc cgattctttc
     3541 ccagcaaggt ataaaggata agcagtaatc cttaataaaa ttgatgccag tatccacaaa
     3601 ataattccaa caaaactacc ttccaatgcc atattagctg caattgctcc aaaaatagtc
     3661 catggaataa ctacaaatac actatctcca attcccgcta aaggacccat tagacctgtt
     3721 ttcagagcac ttactgcttc caaagactta tttttttctt ctttttcaat tccaacattc
     3781 ataccaataa tgtaaggcgc tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa
     3841 acggattgtt tcattccttc aggatcatct ttatatgtgt ccttgataaa aggaagcata
     3901 gaatacgcat aacccaaccc ttccattttt tcataattta atgaagttgc accattgaga
     3961 atccaccgga aaattacttg tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa
     4021 acctcctgtt gcagctggtg ctacttgagt attatttgat ctatttttat atacgattag
     4081 tgcgattgca gcgccaagta acgaaatccc taaaattggt atttggaaat aaactgccaa
     4141 aacgaaacca actaacaaat aggcaaagta atcttttgta ggtaaatatt taagtagcat
     4201 tcctatccca acagcaggta atagtccccc tgctaccttt aaaccaccgg taacccattc
     4261 aggaatataa ttgattgcac ttcccactaa cgttgggcct aatatcacag ttaacaaaac
     4321 aggaattcca gatacaaagc ttgtattaat tgatgccaaa tattgcatcc actctaattt
     4381 ttgataatct tctttttgtg cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga
     4441 ccactttaga acatctagtt gtacaattaa cattgctacc ggtattgcta aagttattcc
     4501 tatagaagca tcctggttag ttgagattgc taaataagta ccgatcaatg ctgctgtttg
     4561 ataatccggt actgatgcac caccaaaact agtaatacct agtgtcatta attgcaaagt
     4621 cccaccgata taaagtccag tttgaatatc tcccataata agtccggtca agaaacctgc
     4681 aacgacaggt tgggaggttc cgattttggg cccttcctta tcccagttaa tgaaaaatcc
     4741 atacaacaca actaaaaatg tttgccaaaa cgccattttg ttctctcctt tcttcttata
     4801 cttttatata agcaattact gtgccaacta acattatcca ataaagcggg aaaatacagt
     4861 aaatattaat taaatgagcg tctaaatata ctttggacac cctttcattt ttttagacgc
     4921 tagtatgcta tttagaaaat agtctgctca aaaattgtat agattaactc atactctcct
     4981 ttgggtattt caatacagta aatatctttg atttctgaaa aaaaatcttt tgatacattt
     5041 aaaaaagtat caaatagttg cgctccctct ttcaaatgtt catttgttat tccatcacca
     5101 atcataattc gttcaatcat tccagaaaga tgtagaaata gattaattct cagaaaactt
     5161 gggattttga catcgtaata tttttcatac aaatatagta ctttttctat ctcattgaat
     5221 acttttttgg aatctaaaaa gcgaagctta gtactagcgc cttcaatagt aaagagttta
     5281 actaattcat ttaa
//
LOCUS       DANKAY010000152         5291 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000152 DANKAY010000000
VERSION     DANKAY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="PWY98_002760"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="PWY98_002760"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="PWY98_002761"
     CDS             complement(580..1353)
                     /locus_tag="PWY98_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2038449.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="PWY98_002762"
     CDS             1497..1847
                     /locus_tag="PWY98_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2038450.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="PWY98_002763"
     CDS             1828..2544
                     /locus_tag="PWY98_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2038451.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="PWY98_002764"
     CDS             2544..3992
                     /locus_tag="PWY98_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2038452.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="PWY98_002765"
     CDS             complement(4062..4571)
                     /locus_tag="PWY98_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038453.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="PWY98_002766"
     CDS             complement(4561..5286)
                     /locus_tag="PWY98_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038454.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1787 a          929 c         1062 g         1513 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt t
//
LOCUS       DANKAY010000153         5230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000153 DANKAY010000000
VERSION     DANKAY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            239..1954
                     /locus_tag="PWY98_002767"
     CDS             239..1954
                     /locus_tag="PWY98_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2038455.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1957..3720
                     /locus_tag="PWY98_002768"
     CDS             1957..3720
                     /locus_tag="PWY98_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2038456.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3760..4296)
                     /locus_tag="PWY98_002769"
     CDS             complement(3760..4296)
                     /locus_tag="PWY98_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2038457.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4619..5041)
                     /gene="mgsA"
                     /locus_tag="PWY98_002770"
     CDS             complement(4619..5041)
                     /gene="mgsA"
                     /locus_tag="PWY98_002770"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2038458.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1585 a         1039 c          998 g         1608 t
ORIGIN      
        1 cagaattcat ttatagaaaa attaagtaaa atagacgctc ctatgaatga agagactggc
       61 atgttttctc attttctttt ttttaaaaaa aatagtctat tcaatttcta aagcttcttc
      121 ataataaaac tttcatttat gaaaaggctt gttctctttt cattctaaat ttcctaagct
      181 acaatgtggt agtacttgtt ttttcaattg tggacaaata aataatagga ggaatcatat
      241 gattcagtta ttacgttatg caaaagacta tcgtaagcaa atcattttgg gcccgttctt
      301 caaatttctt gaagctgtct tcgaacttgt cctgccttta atgatggctt ccttgatcga
      361 taatggacta aaaatgaatg accgaggaaa aatcattgaa atgggcttat ggatggtcgc
      421 tatgtctgtg attggtctga tttgtgctat catttgccaa tactatgctt cagtagcttc
      481 acaaggattt ggtacagagt tacgtaatca gctaatcaaa aaaatcaata ctttctctca
      541 caacgagttg aatcattttg gaacagatac cttgatcacc cgaatgacaa acgacatcaa
      601 tcagcttcag ctagctttag caatggtcat tcgtttattg atcagagcac cgtttttaag
      661 tatcgggtca gtggtcatgg cttttgtgat cgactgggaa gtaggtctgt ttttcttagc
      721 attattgcct attttctcaa tcattttatt ttttatcatc aaaaaaaccg ttcctctcta
      781 ccaaaaagtg caagaaaaac tagatcagct caatgaacac gttagtcaga atcttagcgg
      841 tgttcgtgtc atccgcgcat ttgcgcagac aaaaaaagaa acagagaagt tcgataaaag
      901 cacaaatgat ctagcgaaca attatttacg tgtttccaat atctctgccc tattatcacc
      961 tgctacaacc ttgatcatga atgtcgggat catctgtttg ctgacagttg gcgggatcaa
     1021 agtaaatatt ggtagcttgc aacaaggaca agttcttgct ttgatcaatt atatgaatca
     1081 aatgcttttg gcactgatcg ttgtttcaaa cctcgtcgtg atcttcacac gtgccgaagc
     1141 ttcaggaaat cgggtgaaag aggtgctaga cacagaaaat tcgattcttg aagctgaggt
     1201 cagtactact cccgattttt cttctgaagc aattatcgct tttgaccatg ttgacttccg
     1261 ttatactcct gattctggtc tttctctcca aaatattaca tttcagctga agcgaaatac
     1321 agtccttgga atcattggtc caaccggaag cgggaaaacg actttgacac aattgattcc
     1381 gcgtttctat gatgtcagtt ccggtacagt tataatggac ggaatggatg ttcgttcttg
     1441 gcagctggat catctccgtc agcaaattgc tcttgttccc caaacttctg tactatttac
     1501 cgggactatt cgtgaaaacc ttcaatgggg taagaaagac gccacagaaa aagaatgttg
     1561 ggaagctctg aaaatcgctc aggcagaaga atttgtccgt cagcttccaa atggtttaga
     1621 tacaaaagtc atggaaaatg ggaaaaattt ctctggcgga caaaaacagc gactgacgat
     1681 tgcaagagct ttgattgcca agccagcatt actgatttta gatgactctt taagtgcgtt
     1741 ggattatcaa acagacttgg atttgcgtaa ggcgctacag acttatcttg gcagtacagt
     1801 gattattatc tcccaacgtg tccgctctat tcaagaagca catcatatct tagtgatgga
     1861 tcaaggtaag attgctgctc agggtaccca tgaagaatta cttgctcaat cagcagaata
     1921 tcaggaaatc gtcgcatcac aggaggaaga atagttatga aagtaaaaaa attatccttg
     1981 cagacgctcc gccgattcat tccttacctt actgcctatg caaaagacat ttggctagct
     2041 gtgatactag gaatcatcaa tgggattgca acagttgtga tgacgttaca aattggtcaa
     2101 agtatcgatc aaatgattgg agaaggacag gtccgcttca atcgactgtt ccatttactt
     2161 attctattcg gtggtatcgt tttaatcaac gtagtcagtc aatggctcat ccaagtgtta
     2221 agcaatcggt tagcttactt atccgttgca aaattaagaa aagatgcatt tgctcatctg
     2281 aatcgattgc cacttcgtta ttatgatcaa aactcccacg gaaatatcgt cagtcgattt
     2341 acaaatgata tggacaatat ttccattgca atttctgcgg ccttcaatca attattttct
     2401 ggagtggcaa tcatcattct ttcgtttatt tttatgatgc gtctaagtcc acttctgaca
     2461 ttggtcgttc tttgttctac accaattatt ttccttgtga gttggatcgt tgctcgtgct
     2521 tcacaaacaa atttcgatag acagcaaagg attattgggg atatctcggg ctttgtgacc
     2581 gaacgaatcg gtaaccagaa aatcgtgaaa gcttttcagc aagaagaagc tagtcaacag
     2641 caatttgaac tgctaaatgc tgatctatat gaaaaaggac aacgggcaca attttcttct
     2701 tccttgacaa acccgtcttc tcgttttgta gatcaccttg cttatttatc gattggttta
     2761 gttggcggcc tattggcatt gaagagtggt tcgtcaatca ctgttggtgt gatttccagc
     2821 tttacgatct actcaagtca gttttctaaa ccatttatcg aactttctgg aatcacgaca
     2881 caactgcaaa ccgctttttc tggtctagaa cgagcatttg atttgatcga tcagccagaa
     2941 gaaaagcctg atttgccaga tgcctatgcc ttggaaccca aaaatatcaa aggaaaagtc
     3001 tcttttgatc atgtttcttt ttcttatgac cccaagcagc gtttgatcga agatttcaat
     3061 tttactgctt atccaggtca aaccattgct atagtaggaa aaacaggggc cggaaaatcc
     3121 acacttgtta atttgctgat gcgtttttat gacacaacaa aaggaacaat tacagtcgat
     3181 gaccatgaca tccgctctat ccaacgtgat acattacgta aaagttttgg aatggtgtta
     3241 caagatactt ggctatttga cagtacattg cgagaaaatc ttcgttttgg gaatccggaa
     3301 gcaagtgacg cagaaatcaa tgatgcccta aaagcctctt atatgtatga atttgtagaa
     3361 cgtttgccgg aaaaattaga tactaagatt ggcggtcaag ggttaaagat atcagatgga
     3421 caacgccaac tgttgacgat tgctcgaacg atgatcagta atccccctat gttgatctta
     3481 gatgaagcta ccagctcagt cgatacattg acagagaaaa aaatccaaca agccttctta
     3541 gcaatgatgg aaggaaaaac cagttttgtg attgcccatc gcctgtccac aatcaaaaat
     3601 gcggaccaga tattagtgat ggatcaggga gctattgtcg aaatgggcac ccatgatgag
     3661 ctgcttcaaa aagatggtta ttaccgacgg ctctatcaag cacaattcac agcacaataa
     3721 aaacaagcag tcttctggga tgggaagact gcttgttttt tatttagcgt ggatcgctaa
     3781 acttcttaaa atttttttgt aaggaatact acgtcctacg taataaatgg caattgattg
     3841 aatcggtaac catattactg tttttaacag gcggggcgcc caaacgaccc agctgaacaa
     3901 tggcacatgg tacatcaacg caagccataa tggtgtcaaa aataagttga tgatcagcgt
     3961 gacactaagc gtagccagaa tggcattttt ccaagtgatc tcttttcgat agaaaaagaa
     4021 tccgtagatt gctccttcaa taaatgcatt caatgtaaat ccaataaaga atgcagattt
     4081 cgagaagagg gccattccta ctaaatcagc taaaactgcg cctatccctg accaaaaagg
     4141 accaaaaaga atgcccatga tcacttgtgg aataaatgtg acactgattt taataaatgg
     4201 ggtctcgatt gctaccagtc gggacaaagt gaccatcaat gcaatcaata agcccataat
     4261 agcaatcata cgtgcgtcta accggtgttt cttcattttt agcatctcct ttaagagctg
     4321 ctacatcgta tcatgcagcg aatgcgaaaa taaaaccgcg aacattacgt ccttcgtcca
     4381 agaggcaaca tcccatcctc ttagcactta acgctttatt ttctactctg tattattttt
     4441 gggatctttt cccacagaaa ctatagcata ttttcacatt tactgcaaat acgaaaaact
     4501 aatattattg tttatattat ccaatacgtt cgtattatat cgcatattat cagtttgttt
     4561 agcttttata gctacatata ggcgaacatt aaacgttatt tttccatacg cttatccatc
     4621 aaaatgaaag tggatgcttg tctatttcat ggacgattct tcgaaaatct aggaagccgg
     4681 cttccagtcc tcttagtagc acctcagctg ttccgatatt cgtagctaac ggaatctcat
     4741 agacatcaga taaacggata agtgctgtta catctggttc atggggctgt gcagccagcg
     4801 ggtctcgaag aaaaatcacc aaatctaact gatcctccga aatcatggct ccaatttgct
     4861 gatccccgcc taacggtccg gatttaaacc gatggatcgg caagccggtt gcttccgtga
     4921 tcctctgccc agtcgtcccg gtagcaaaaa gttcatgttt ttccaatatt gttttatacg
     4981 ctgttgctaa tttcaccatc ataggttttt ttcggtcatg agcgatcaaa gcgattttca
     5041 taagagacac tccttttgat attgttaaca tacttattta ctccagtgta tcagttttta
     5101 tttcttatgg atatttatta aagcttaaaa ctagcacatt gacaaaaaaa agaaaaaatt
     5161 cttgttcaac atgacattga agttagttaa cgctttcatt tatactttaa atagttaata
     5221 aaactaaaaa
//
LOCUS       DANKAY010000154         5190 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000154 DANKAY010000000
VERSION     DANKAY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            137..748
                     /locus_tag="PWY98_002771"
     CDS             137..748
                     /locus_tag="PWY98_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2038459.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="PWY98_002772"
     CDS             821..1750
                     /locus_tag="PWY98_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2038460.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="PWY98_002773"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="PWY98_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="PWY98_002774"
     CDS             complement(2693..3871)
                     /locus_tag="PWY98_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2038461.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..4067
                     /locus_tag="PWY98_002775"
     CDS             3894..4067
                     /locus_tag="PWY98_002775"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2038462.1"
                     /translation="MCCGNSILLKGLPFFYLHKIFYTLGLKERGLSGVKMVISDAHIG
                     LTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="PWY98_002776"
     CDS             4098..4499
                     /locus_tag="PWY98_002776"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2038463.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5190)
                     /locus_tag="PWY98_002777"
     CDS             complement(4706..>5190)
                     /locus_tag="PWY98_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2038464.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1640 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta
//
LOCUS       DANKAY010000155         5176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000155 DANKAY010000000
VERSION     DANKAY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(136..1098)
                     /locus_tag="PWY98_002778"
     CDS             complement(136..1098)
                     /locus_tag="PWY98_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2038465.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="PWY98_002779"
     CDS             complement(1100..2539)
                     /locus_tag="PWY98_002779"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2038466.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="PWY98_002780"
     CDS             2724..4670
                     /locus_tag="PWY98_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2038467.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="PWY98_002781"
     CDS             4934..>5176
                     /locus_tag="PWY98_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2038468.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DANKAY010000156         5146 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000156 DANKAY010000000
VERSION     DANKAY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..244)
                     /locus_tag="PWY98_002782"
     CDS             complement(<1..244)
                     /locus_tag="PWY98_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL2038469.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAE"
     gene            complement(608..3274)
                     /locus_tag="PWY98_002783"
     CDS             complement(608..3274)
                     /locus_tag="PWY98_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2038470.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3615..5066
                     /gene="cls"
                     /locus_tag="PWY98_002784"
     CDS             3615..5066
                     /gene="cls"
                     /locus_tag="PWY98_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2038471.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1493 a         1095 c          908 g         1650 t
ORIGIN      
        1 tttctgcttc tttttttgca ttttctagca ttcctgaaga aaaatcgatc aaatcatagc
       61 gattaatttc ttttgctaag gaaagaaatc tgccagatcc tccagcaacg tctgctgctg
      121 tttcagcagg caggactttc tccgttttca gataattttt tacatcttca gcaatagtta
      181 cttgcgattc ttgctggatt tcataatatt cttcagcaaa ttcatcccat tctttttcat
      241 tcatttgatt tcctctaatt ttttgttata tgttttcact tttcggaata aatcattctc
      301 ctcttttatc tattctatct cttttgatga ataataaaat gattggcttt ccgtctatca
      361 taaataaaaa aagggactgt gaccaaagtc gtgtcaaagt ccctctttcc gaataaacag
      421 tgtccaatca gatgaccctc agtattagct caaaatgctg attttacaac ttcttcctta
      481 aacggtgttc tcctgccaac tcttcggtca caagtcacaa tattcaacaa acatgagaag
      541 ctgtttgttg aaattgcacc ttgtgactca gaattaaacg gcaggctcac aacctcgttg
      601 attcgtttta tttatttcct tgtactcgta caatgatcaa tttagtgatt tccatcaaaa
      661 caattgtcac aagtgcgatt cccatacatg taaagacttc tgtcaatcca aatgctgatg
      721 ggatagagaa gacttctttc agtcctggga tcattgttaa gcagtaaagc gcgctacata
      781 caactacagc tagtaagaca cttctgttag agaagaaccc tgcttggata gctgtttgtg
      841 tgtttgaacg tgctgggaat gtttgcaatg tccgacacca aatcaatgtt gaaaatgaca
      901 tggctacacc gaattcaggt gaatgctgca tgccaagcca ttgagcaaag ataacagcaa
      961 tcccaatcag cacaccacga tacgttacag aaatcaatgt tttgtccgta aagattcctg
     1021 aatttggatc tcttggcggg cggttcatcg tagcaggttc agctttttct gtacctaatg
     1081 caattgctgg cagtgaatca ttgactaaat tgatgaacaa cagttgtaat gctgtaaatg
     1141 gattggacca gtcagcaact aaggcaaaga tgatcgcaat gatcgcacct aaatttcctg
     1201 caaatagata agaaatagct ttcttgatat tatcaaacac attacgtcct acttctactg
     1261 cgctgacaat agaagcaaag ttatcatccg tcaataccat cgctgctgca tcttttgcca
     1321 catctgtacc cgttcccata gcaattccga tgtctgcttg tttcaatgct ggtgcgtcat
     1381 ttacaccatc acctgtcata gctgagatat gacctttatt ttgccaagca cgaacgatac
     1441 gaattttatt ctcaggtgaa acacgtgcat aaacagtgac tttttctaaa acttcgttta
     1501 gttgatcttc tgtcaattca tctagttccg tcccggtcaa agccaggttt ccttcttcaa
     1561 aaatcccgat ttgttttgcg atagcaacag ctgttgtttt gtggtcacca gtaatcatga
     1621 ttgttttgat tcctgcactt cttgcttctg ttaccgcctg agtcacttca gcacgtggag
     1681 gatcgatcat agcaagcaaa ccaacaagga tcaaatcgtc ttcatcttca aatgataaat
     1741 caaaattatc aagtggacgg taagcaaaag ctagtacacg taatgcgcgt gttgaaaaag
     1801 cttcattttg ctcttggaat tttttcatca attcatccgt catcggaacg acttcaccat
     1861 ttagcaatac ttttttactg cgttggaaga cgatatctgg tccgccttta acaaacaagt
     1921 aatttttgtt atcaattgtg tgtccagtag acattaattt tcgatctgag tcaaaaggaa
     1981 gttctgcttg tcttgggtag ttgtttcgga tttcctgata aggctggttt aatttttcac
     2041 taaagtcgat aaacgctact tccgtagggt cgcccaattt agtaccgtct tcactgatcg
     2101 atgcatcatt cgctaaaaca ctgatttcga tcaagcgacg ttcgtcagct gtccattttg
     2161 aagggtcatt tgtgaaatcc ccagtatcgc cattacctaa aaagtagtca acaactgtca
     2221 ttttgttttg tgtcaatgta ccagtcttat ctgtacagat gatacttgtc gaaccaagtg
     2281 tttcaactgc tggaagcttc cgaataatgg catgacgttt tgccatttta ttcgttccca
     2341 aagataacac gattgttacg atagactgta atgcttctgg aatagcagct acggctacgg
     2401 ctacggcaaa catcactgcg ctgacgatat ctgcagtcat atctcctgta ccttctccaa
     2461 gataaatacg aaacagttgg atacctaaaa tcaataaaga caatgccaaa atagccaaac
     2521 ttagtttttt actgaattta tccagacctc gttgtaacgg agtgacttgc tcagttgttg
     2581 attccaaaag gccagcaact tgtccgatct ccgtattatt cccagttgaa gtcactaaga
     2641 atttaccgcg tccgtaagtt acgatcgttc cactgaatac catatttgta cgatctccga
     2701 ttggtaccgt acttgtgata tccgttactt ctttttctgc agaagtcgac tctcctgtca
     2761 gcataccttc atctactttt aatgatcccg catctagtaa acgtccatca gcaggtacat
     2821 aatcgcctgc gtctaacaac acgatatctc ctaccacaag ttcgtttgcc ggaatcgttt
     2881 tttccttacc atcacgaatc actcgagcat cgggcgctga aaggtttttc aacgcttcta
     2941 aagaaccttc tgcttttttt gtttggacaa cactgattat agaattcaac atcaatactg
     3001 caaaaataac taagaattcg atgtagtcac ccatgaccat ctgaatcagt gcgacaatca
     3061 gcaatacgat aaccattgca tctttgaaag tttctaagaa caatttccaa gttggcttct
     3121 tcggcttttc ttttagctgg ttcaagccat ctctttctaa tttttccttc gcttgcgtgg
     3181 tgctcaatcc atcggttgag ctctgcgttt gctgcatcaa cgagtcgatc gtttctttat
     3241 agaccgcttg ttgtgcttgt tccttgtttt ccaaatttca tcccccattt tttgatcttg
     3301 gccctagtcc tgtctgaaaa aaaagagacc taagacaaat acgcatttgt cttaggtctc
     3361 accatttcat acaataccag aaaaccattt ggttaactta ctgttgatat tatcgcaaca
     3421 aacgttgccg gttactcccc taaggagata ttcaactgta ctaatatcct accttattaa
     3481 cctacaaaaa gcaactactt tagcccgatt tacctacatt ttcatgaaag gagtgaaaag
     3541 tatattcacc ttggtaggct tctttcttct atcatgctat actaatgtta ttgtatgtaa
     3601 ggaggaacat ttttgtggta tctagtatta taaccgccct ttatctatta aatgcactta
     3661 ttgctttggt ggctattttg atcaaacccc gagatgtagc agccatttgg gcatggctct
     3721 tagtattgtt tgcccttcct ggggtgggct ttgtattata tttatttttc ggacgtggat
     3781 taacggataa gaaaaaattc tatctccgac aaagtgactt gaaagaatta gaaaactttc
     3841 agtcttttag aggagatacc attgaacatt acgatcctga catgggcgat aaagacaaac
     3901 agcaatttgt tgacttcttc tcttcattaa atcgtatgcc gctgacaaga atgaattctg
     3961 tcactcttct cacagacgga caagagaaat tggattcact gcttcaagat ctaaaaaaag
     4021 ccaaacattc gatccatatc gaatattacg catttgtgac agataatatc ggccagcaag
     4081 tcttacatgt tttagaagaa aaagccgcag aaggcgtgga agttcgaata ttatatgatg
     4141 catttggctc tcatggcaca aaagcaaaag atttcaatcg tctaatcaaa aatggtggac
     4201 atgtccatac atttgttacc tcacaaaggg cattacttcg tttccgattg aattaccatg
     4261 atcaccgaaa aatcgttgtg atcgatggaa agattagtta taccggtggt ttcaatattg
     4321 ccaatcaata tgtaaataca acaaaaaaat tcggctattg gcgcgatacg catatacgga
     4381 ttttcggtgc cgcttcttct ttgctccagc ttcgcttctt aacagactgg aacgtctcgg
     4441 tacctgaaga aaaaaaggtc ggctatcatt tgaattattt ctttaaaaaa gcagatcgag
     4501 atgaatctaa gcttgctgat acatccatcc agcttgtttc aagcggaccg aataacgaaa
     4561 gggaacaaat caagctttca tttatcaaat tgattacttc tgctaaaaaa cgtgtttgga
     4621 tacagacacc ttaccttgtt cctgatgaaa gtgtcattgc tgctttaaaa atcgcaactg
     4681 cctctggtgt agatgtgaaa atcatgattc ccaacaaacc ggatcatcct tttatttatc
     4741 gagcaacaca atattatgct cggcagctga tcaaggaaaa tgtacaaatc cttgtctatg
     4801 agaacggctt cctccatgca aaaacattga taatggatga tgaaatctgc atggtaggtt
     4861 cagcaaatca agatattcgt agctaccgat tgaattttga aacaagtgct gtcatttacg
     4921 atcctgagtt tttagaagaa cttgctactc agttcaaaga agatgagaca cattgttcat
     4981 ccatgacaac tgaaacagtc aaggaaatgt ctaactggct attattcaag caacaaattt
     5041 ctcgattatt ttctccaatc ctataatttt tttattcaag tagagaaaaa tatactaaga
     5101 ttaaagggac tgtgacaaaa atcttgtcac agtcccttct tccgaa
//
LOCUS       DANKAY010000157         4894 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000157 DANKAY010000000
VERSION     DANKAY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            46..933
                     /locus_tag="PWY98_002785"
     CDS             46..933
                     /locus_tag="PWY98_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2038472.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1073..1999
                     /gene="cysK"
                     /locus_tag="PWY98_002786"
     CDS             1073..1999
                     /gene="cysK"
                     /locus_tag="PWY98_002786"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2038473.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2235..2681
                     /locus_tag="PWY98_002787"
     CDS             2235..2681
                     /locus_tag="PWY98_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2038474.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2954..4306
                     /locus_tag="PWY98_002788"
     CDS             2954..4306
                     /locus_tag="PWY98_002788"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2038475.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4477..4764
                     /locus_tag="PWY98_002789"
     CDS             4477..4764
                     /locus_tag="PWY98_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038476.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1678 a          813 c         1026 g         1377 t
ORIGIN      
        1 aagagaactt tttattaagt ttttttattt tgaggaggaa tttgaatgca agagctgaag
       61 acccgcagtg cccggcacca tagacctaaa agaaaaatgg tgatcaataa aaaagcgatt
      121 gctcctttaa ttggaacgag tcttttagtt gggccgatga tggttacagc ggtaccgcaa
      181 catgttcatg cagatgaaca aggatctcaa acagtcgctg atcctcaagc gtttatcgat
      241 caaatcggct ggtctgcgtc agaagttgct gcaagtaatg acttgtatgc ttcagtcatg
      301 atcgctcaag cgttgttgga aagtgcatat ggcacttccg gattagctag tgcgccaaat
      361 tataatctat tcggtgtgaa aggaagctat aacggacagg tcgtttatat ggcaaccaaa
      421 gaatatgtaa acggacaatg ggtaacgatg aatgagccat tccgaaaata ttcttcttat
      481 tgggaatcct tccaagacca tgcgaatgtt cttcgttcaa ccagtttttc cacgggcgat
      541 tatcattatg ctggcgtgtg gaaaagcaat acaacaagtt tttatgatgc aacagcgtac
      601 ttgacaggtc gttatgctac cgatccaaat tatggggcca aattgaactc attgattaat
      661 acttataatt taacccgttt tgatacgcca tcaacaggtt cggctacaac aaccacaagt
      721 acaacaacaa cgactactac gacgacttca acgtcatcat cttcaacaac agatgtaaat
      781 acaaataatg gtcagacata tacagtcgtg tcaggtgata cgctatggga tattgcacaa
      841 aaatttggca tgtctgttga tgatctaatg gcgaaaaatg gcatgtctat gtccagttat
      901 tctttattag ttggtcaaga actaaacgtt tgatttagat aaaaaggcaa agaaattacc
      961 atatatggta tatctttgtc ttttttttgc tagtcaaaga cgaatcactc gccataaatg
     1021 aacagattgt attatgataa gtaagaagag caaagacaag gaggaattga agatgacagc
     1081 tatctataac tcagtcaccg aattgattgg caaaacgcca atcgtcaaat taaataaaat
     1141 cgtaccagaa gattcagcag atgtttttgt aaagcttgag ttttttaatc caggaggaag
     1201 tgtaaaagat cgtattgctt taagtatgat agaaaaagca gaacacgatg gtctactgaa
     1261 accaggtgat actatcattg aacctacttc tggaaataca ggaatcggat tgtcgatggt
     1321 aggagttgca aaaggatata aagtaatcat cgtgatgcct gagacgatga gtatcgaaag
     1381 acgtttattg atgaaagggt acggcgcaga gttgattttg actcctggtg cggatggtat
     1441 ttctggatca atcagagaag cagaacgttt ggcaaaagaa aacggctact ttttaccatt
     1501 acaatttgaa aatgaagcaa atcctcttgt gcatgagaag acgactggtc ctgagataca
     1561 tcaagcattt ggagtaaatg gcttggacgc gtttgttgca ggtatcggta caggaggaac
     1621 gatcactggc gctggccgag aattaaaacg tgtgtatccg aaaatcgaat taatcggggt
     1681 agaaccagca gaatctgcta ttttagaagg gaaagaagcg ggacctcaca aaatccaagg
     1741 aatcggtaca ggttttgttc ctaaaacact ggatacttct gtgtatgata aagtcctttc
     1801 tattagtgga gacgaagcga tggaaacagc tagagaagtt gggcgaaaag aagggatttt
     1861 agtaggcatc tcatctggcg ctgcaattgc tgccgcgcta aaagtcgcaa aagaattagg
     1921 aaaaggcaag aaggtattag ctgtggttcc cgacaatggt gaacgttatt tatctacagc
     1981 cctttaccaa gagatataag gcacttggtt tttctccaga taggtttatg taaaaaactt
     2041 ttaaagatga aaagaacaca caaaaattta tgatgagaaa aattatttga atagagacgt
     2101 agaatagtga acgcgactgt tttacgtctt ttttcattaa aatacaataa aattgaattt
     2161 tcttttcatg ttaagtggat tttaaaatgt aaaaatagta tgataagacg agaataacaa
     2221 cgtggggtgt ttacatggaa aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag
     2281 agaacattcg aatcacacct caacggtacg cggttttaga atatttgatt gaacatcatt
     2341 cccatccaac ggctgatgag atttataaag cacttgaaca tcggtttcca aatatgagtg
     2401 ttgcgactgt ttacaataac cttcgcttat ttacttcgat tggatttgtt caagaaatga
     2461 agtacggcga tgcttctagc cgatttgatt tcagttcaaa gcgtcattat catgtaattt
     2521 gtgaaaactg cggtaagatt gttgattttc attacccagg gcttgaagat atagaaatgg
     2581 ctgcaagcag actgactgat tttgagatta atgaacatcg gttggaattg tatggactct
     2641 gtccggattg taaagagtca gcaaaaaagg cgcaaatgta aaaacagttt ctacaagtgt
     2701 tatacctaaa aaaagctagc tgtgtaaaaa aaacgctagc ttttttattt ttttgtcttt
     2761 tattcacaat cttagatata aaagtgaata ttattaaaat aagaatcttg tagatattgg
     2821 gttcttatgc ttgaaaagga atgaaactac aaacaaacct ttgtgaattc acaagcatta
     2881 aactgttgta tccgttttat ttctgttata gtagttttgt cgaaggacaa aatgttaatc
     2941 ggagggacca taaatgaaag tagtagtaat tggctgtact catgcaggta ctgcagctgt
     3001 aaaaagtatt ttaaaaaatc atccagaagc agaagtgaca gtatatgaaa gaaatgacaa
     3061 tatttctttc ttatcttgcg gtatcgcact ttatgtcggc ggagtagtaa aggacccagc
     3121 aggcttgttc tactcaaatc ctgaagaatt atcttcatta ggagcaacag ttaaaatgga
     3181 acacgatgta acagacgtcg atacagaaaa taaaacagta acggcaaaag atctgaaaac
     3241 aggagaaaca gaaacggttt cttatgataa attagtgatg acaactggtt catggcctat
     3301 tatcccaccg attagtggaa tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca
     3361 agcaaatgaa attattgcac aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg
     3421 atatatcggt attgaattag tagaagcttt tgtcgaatct ggaaaacaag tgacattgat
     3481 tgatggatta gatcgcatct tgaataaata tctagataaa ccattcacag atatcttaga
     3541 aaaagaattg acagaccgcg gtgttactct tgcgctaggc gaaaatgtta ctgaatttat
     3601 cacagatgaa gaaggaaaag tgaaacaagt agctacacca acagatacat ttgatacaga
     3661 tatggtcatt ttatgtgtgg gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga
     3721 tacactgcca aacggggcaa tcaaagtgaa tgaatacatg cagacaagta atccagacat
     3781 ttttgcagct ggagactctg cagtagtcaa ctacaatcca agtggcacgc aaaactatat
     3841 tcctttggca acaaatgccg ttcgtcaagg tttgcttgtg ggaaataact tgacagatca
     3901 taaaatggcg tatcgtggta ctcaaggaac ttcaggactt tacctatttg gctggacgat
     3961 cggttcgaca ggtgtgacca aagaaagcgc aactttgaac gatctagaag tccaagcgac
     4021 tgtatttgaa gataattatc gtccagagtt catgccaact actgaaaaag taatgatgga
     4081 gctagtctat gaaaaaggaa cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga
     4141 cattacacaa tcagcaaata ccatgtcttt agctgttcaa aataaaatga ctgtagaaga
     4201 tctcgcactt tcagatttct tcttccaacc acattttgac cgcccatgga attacttgaa
     4261 cctacttgca caagcagcgc taggcgaaat cgaaactgca caataaagaa aaagggttgt
     4321 gacacaattt tgtcacgacc ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc
     4381 ttcgaatttt ttgtcacaat aagcagcaaa gataaagctg ttacataaaa gaacaagcag
     4441 ctttatttga ttttgaaaag aaaggtgaga aagaagatga ataaagaata ttttgtccaa
     4501 caagatgcat ttttagaaga ttataaaagt gcttcgaaga aattaacgat caatattgat
     4561 gagttggcag atcaagtact ctctcagttg aatgaaacgc aagaggaata ttacacgatg
     4621 ccagcaaatg acgccaaaga taatgtttct catcgttttc ctttcagtag acgcatctac
     4681 tcagatgaaa atgacggcac attgaataca gagtacacat atgaaggtca gccatatgtg
     4741 ctagaaactg atgatgatct ttaaagacag tttttgaaaa ggccgctcat ccttaagtga
     4801 tgacaaaaaa tcatcacaat taccgatgac tgtctcttta ctatctttta atcagaaaaa
     4861 ggggttatga caagctttgt caaccccttt tttt
//
LOCUS       DANKAY010000158         4881 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000158 DANKAY010000000
VERSION     DANKAY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..562
                     /locus_tag="PWY98_002790"
     CDS             <1..562
                     /locus_tag="PWY98_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2038477.1"
                     /translation="TNDPFAQGTKISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDE
                     IGFIWKAMSIFAKHEISIEHIPSGIDNIGVIVSAEAIADKLFLITKELKETLGVEEIV
                     VIEDLALISVVGGPHKELIGLSGKVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYE
                     TVVKGIYEGMVNTDAERISNGTPSNS"
     gene            522..1673
                     /locus_tag="PWY98_002791"
     CDS             522..1673
                     /locus_tag="PWY98_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2038478.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            1648..2817
                     /locus_tag="PWY98_002792"
     CDS             1648..2817
                     /locus_tag="PWY98_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2038479.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            2992..4371
                     /locus_tag="PWY98_002793"
     CDS             2992..4371
                     /locus_tag="PWY98_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2038480.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            4652..4881
                     /locus_tag="PWY98_002794"
                     /pseudo
     CDS             4652..4881
                     /locus_tag="PWY98_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1637 a          852 c         1052 g         1340 t
ORIGIN      
        1 taccaatgat cctttcgctc aagggacaaa aatatcgaat cacgaaatag aaaaggaaaa
       61 tggactgaca ggaatagctg gacgcaagga ttttctttcc atcactttat tcaaacgtca
      121 tatgtccgat gaaatcgggt ttatctggaa agcaatgagc atttttgcta aacatgagat
      181 atcaatcgaa catattcctt cagggatcga caatatcgga gtgatcgtct cagcagaagc
      241 gattgccgat aagttatttc tcatcacgaa agaattaaaa gaaacacttg gcgtggaaga
      301 aatcgtcgtg atcgaagact tagctttgat ctctgtcgtt gggggtccgc ataaagaatt
      361 gattggatta tctgggaaag tgttatcgat attgaacaaa ttagaaattc gcacatcgat
      421 cttatcacaa ggagcacagg agttgaatct gatcatcggt gtaccgaata atcaatatga
      481 aacagtagtg aagggtatct atgaagggat ggtgaatacg gatgctgaaa gaatatcgaa
      541 tggcactcca tcaaattcct gaaatcgggt ttcatgaatt caagacaaaa aaatatttat
      601 atgaacaagt aaaaaacagc gaggccgtta tccatgaaat cggcgaaaca ggactactcc
      661 tttattttga taatcagcaa gaagaaacga ttgcttttcg aacagatatc gatgcactgc
      721 cagtcaaaga agaaacgaat ctctcttttg cttctaagca tgaaggttat atgcatgcat
      781 gtggtcatga cggacatatg gcgatgcttt tagggttgac tgactttata actaaacaca
      841 ttgaggatct tatttataat gtagtactga tctatcagcc atcagaagaa atttccggtg
      901 gcgcacaaag cgttattcag tcaggattgt tggataaata tgaagtacaa gcaatatttg
      961 gctttcatct atggcctgga ctagctaagg gagaagtttt ctctcgacca ggacctttga
     1021 tggcacaaag cagtgaaaca gacattatca ttaaaggcca gtctgcacat attgcttcta
     1081 gtgaaaaagg aatcgatagt ctggaggcag ctgtacgatt tttgaagaat gtttatgatt
     1141 ttgaccggca attaccggca gagtggaatc atctaatgaa atttggccaa atccaaggtg
     1201 gaacgatccg aaatgtttta gctggagaag ttattgtatc cggaagtatc cgttcttaca
     1261 gttcagatat gcaaaattat ttgaagaggg gattagacca tttagcagaa cagtttcact
     1321 ctacagctac tatttctttt cgctataatg acggctatcc agctgtaatc aaccatagag
     1381 gcttatatgc agcactgtta gattcaaaca aactacatga attagatgct ccagttcttc
     1441 aagccgaaga ttttggcata tatacgcaac aatatccatg tgtatttttc tttttaggtg
     1501 tcggtgatac ccctgcttta catgatccga cgttcgattt tgatatggat gttttagaaa
     1561 atggtgtaga atggtacaaa acgattcttt ttacagaaaa tcttcttgta aaaatcgata
     1621 gcagttcaaa agaggagcga gtaaataatg atgaatttat ccaatcgatt tagtcctagg
     1681 ctttctagaa tagaagtatc aaaaatacga caattcgatc agcaaatatc gtcgatacca
     1741 gatgtgatca aactcaccct gggagaaccg gattttccaa ctcctgaaca tgtcaagcaa
     1801 gcaggaatag cagcgattga agaagacttt tctcactata ccggaatgag aggattagag
     1861 gaactgcggg aagcggcttg tatatttcag caacagcgtt atggattaac ttatgatccg
     1921 caaacggaag ttttgacgac agttggtgca acggaagcga ttgcctctgc gttgttatct
     1981 gttttggaag aaggagacaa ggttcttatt ccagcgcctg cttattcagg atatcagcca
     2041 ttagtggaac tagctggtgc tgaattgatc ccgatagata cttctgacac agggtttgtc
     2101 tgtcagccgg aacaatttga acgagcattt gaacaatatg gatcagctgt caaagcagtc
     2161 attttaaact atcctaacaa tcctactggg acgacattgt ctgcaaaaca attgaaagca
     2221 atcgctgagg ttttaaaaaa atatccggta tttgttataa gtgatgaagt atatgctgaa
     2281 ttgacttata gtggaacgca tatgtccata gctacttatc ttccagaaca aacaatcgtg
     2341 ataagcgggt tatccaagtc acatgcgatg actggttggc gagtgggctt tatttttgct
     2401 caaaaagccc ttattgacga actaattaaa gtccatcaat atctggtgac tgccgctaca
     2461 acgatgtcgc aaaaagctgc ggctgaagcg ctaataaatg gggtgaaaga tagcgagaat
     2521 atgaaagagc aatatcaaac acgaagagac tatctggtcc agcaattggc gcctttagga
     2581 tttgaagtga cccagccaaa cggtgcattt tacttgtttt gcaaactccc agctgccatt
     2641 caaactgact cttggcagtt ttgtgtagaa ttagcggaac aggcaaaagt tgcgtgtatc
     2701 ccaggaattg catttggccc agaaggagaa ggttatatcc ggatttctta tgctagcagc
     2761 atggaaaaat tacatgaggc ctgcaaacgg atcaaagact ttttaacaga acagtgattc
     2821 atagatttta tcaaaaaaac aatcgttaat tttgcggtat attctgcaag gagacgattg
     2881 ttttttaaat ttagttcaaa tgaacgcgtt tcttttgatt cataactaat gtaatgttaa
     2941 gtttaaattg tttagaatga ttttaaaaga atattcaagg aggtttcatc aatggaaaaa
     3001 atcaaagttg tcattgtagg tgcatctcat ggagggcatc aatcgatctt ggaactattg
     3061 acacgatacg gtgaaaatgt agatatcacg ctatttgaag caggagacta tatttccttc
     3121 atgtcatgtg gtatggagct gtatttagaa gacaaggtaa cagatgtaaa tgatgtacgt
     3181 aatttcagac cagaaaactt tccgcaatcc aatgttcata ttttaaataa tcacgaagta
     3241 aaaacgatcg atgccgataa aaaaacagta acggttacta gcgcatcaga taacgaaaca
     3301 aaagaatatc cttatgacaa attgattttg agttctggtg tcaaaccaaa caatctgcca
     3361 gtaccaggag cagatctaga aaatgtctac ttgatgcgtg gatacaattg ggcaaccaaa
     3421 atcaaagaaa aattaaatga tccggctatt aaaaaaatcg ctgtcgtagg tgcgggttac
     3481 attgggatcg aagcagcaga agcaagccga aaagctggga aagaagtcgt attgcttgat
     3541 gtgatcgata gacctttagg cacttattta gatgctgaga tgacagatat tttagaacaa
     3601 cacctaaatg aaaacggtat tgaagttttg acaaatgcaa aaatcgaagc ttttactggt
     3661 aacggaaaag ttgaggcaat caaaacaagt gaaaaagaaa tcccagcaga tctagtcatt
     3721 caagctgccg gtgtgaaggc aaatacagaa tggttaaaag ggatcgtcga cttagatgaa
     3781 cgtggctgga tccaaacaaa tgaatacttg caaacgaatc ttccagatgt gtatgcagta
     3841 ggagatgcta cacttgctta ttctatccca gcacgcaaaa aaatgccgat cgctttagcg
     3901 actgttgcac gtagagaagc acgttatgta gtaaaacatc tttttgaaga agtaccaaac
     3961 aaaccgttcg gcggagtcgt aggttcctct gcattaagtg tctttgacta tcattttgca
     4021 gcaagcggat tgaatagctt tacagcagca aaagcaggag tagatgtacg agtgtcttat
     4081 tatgaagata cgttacgacc aaaatatgta ccagaagaac aaggaaatcc aaaagtgtcc
     4141 atccaattat tctcggatcc aatgacccat caattgctag gaggagcagt attatctacc
     4201 tatgatgtta caggtcaagg aaatgtattg gcactagcga tccaaaacaa actgacttta
     4261 gaagaactag cagaagctga cttcttcttc cagccaggct ttgaccgaca atggagcatg
     4321 ttgaatctag ccgctcaaca agcgttagga gaagaaccat ttgtagaata agaaaacgaa
     4381 atatttgaaa agaggttgtg aaaaagctgc cttgcatcaa gtaataagaa cagccaagta
     4441 aaaacagctt cttatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga
     4501 acaccgttta ttttcaaaaa agggactgtg acacgatttg tgtaccgacc cccaaaagtt
     4561 agaccaaaaa atctaacttt tggaggtcgg tattttttat ggttaaatat acttttgaac
     4621 ttaaattaaa gatcgttcat gattatttgg atggcaaagg cgattggacc gtaaaaatcc
     4681 agatcaagag attgaagcgg aaatgcttaa aattcgggag aagcacaagg attatggctg
     4741 tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa agaaagttca
     4801 gcgcttgatc aagaagctag gaataaaagt aacggcatac acgcgtaaat cacgtcgcta
     4861 caattcttat cgaggaaaag t
//
LOCUS       DANKAY010000159         4786 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000159 DANKAY010000000
VERSION     DANKAY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1522
                     /locus_tag="PWY98_002795"
     CDS             <1..1522
                     /locus_tag="PWY98_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2038481.1"
                     /translation="VWLHFGGGNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVE
                     KAYHDYNNDILEIVMSGDGELKKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFT
                     TFTITEKGYSLKDSKGQFFSVVKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMV
                     STDNFSQNGQRFQDSIFTIAEAWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPS
                     ENVKEDLLMMGLEDLDIIHTKKHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIM
                     TDRETVDKADIMKVTTSLNPLHTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEG
                     LPVVENPGIIDPKQFIDEVINKRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEK
                     DGNAENLTFIPIAIAAWLRYLLAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSK
                     IHCAVQEILENKEIFGLDLYQVGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1525..2604
                     /gene="uxuA"
                     /locus_tag="PWY98_002796"
     CDS             1525..2604
                     /gene="uxuA"
                     /locus_tag="PWY98_002796"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2038482.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2678..4105
                     /gene="uxaC"
                     /locus_tag="PWY98_002797"
     CDS             2678..4105
                     /gene="uxaC"
                     /locus_tag="PWY98_002797"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2038483.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4189..>4786
                     /locus_tag="PWY98_002798"
     CDS             4189..>4786
                     /locus_tag="PWY98_002798"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL2038484.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVS"
BASE COUNT         1633 a          663 c         1019 g         1471 t
ORIGIN      
        1 agtatggctt cattttggtg gaggaaatct ttatcgagga tttcatgcag aaattgctca
       61 atgcttagca gatcaaaatg aattagaaac aggagtagtt gtgtgtgaaa catttgatga
      121 agaagtagtg gaaaaagctt atcatgacta taacaatgac attttggaaa ttgttatgag
      181 tggagatggt gaactcaaaa aaagactttt ggcagtaacg gctgatagtt tatattgcaa
      241 tccgaagaat atagcggact atcaaagagt ggttaaatat tttgaagatc ctaaattaca
      301 gtttacaacg tttactatta ctgaaaaagg ttacagttta aaagattcta aagggcaatt
      361 cttttcggta gtcaaagctg atatcaaatc tggacctagc aatcctcaac atacgatgag
      421 tattgtaaca tccttgttat atagcagatt taatgctgga gagttgccga ttgcgatggt
      481 ttctactgat aatttctcac agaatggtca acgttttcaa gatagtattt ttacgattgc
      541 agaagcttgg caaaaagaag gatatgtttc tgaaaaattt attgcttatt taaaggataa
      601 agaaaaagtg acttttcctt ggagtatgat tgatcgaatt acgccaaatc cttcagagaa
      661 tgtaaaggaa gatctattga tgatggggct cgaggattta gatatcattc acacaaaaaa
      721 acataccaat attgctgctt tcactaatac tgaggtagtt cattatttag ttattgaaga
      781 tagttttcca aatggccggc ctaaattgga aaaggctggt gtgattatga cagatagaga
      841 gacggttgat aaagcagaca tcatgaaggt tacgacaagc ttgaatccac ttcatactgc
      901 tttggcagta acaggttgtc tgctaggata tcaatcaatt tcagcagaga tgcaggatga
      961 tgatttggtt ggtttaatca aaggaatagg ttatttagaa ggtttaccag tagttgaaaa
     1021 tccaggcatt attgatccaa aacaatttat tgatgaagta attaataaac gtttgcctaa
     1081 tccctatatt ccagatacac cacaacgaat tgcaacagat acttctcaaa aagtagcgat
     1141 tcgctatggt gaaacaatta aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac
     1201 atttattcct atcgctattg ctgcttggtt acgttattta ctggcagtcg atgatcaagg
     1261 ggaaaatttt gtgccaagtc ctgatccgtt attggtagag ttgcaggata aattatcagt
     1321 attgaaatta ggtgaacaag atccttcaaa gattcattgt gcggtacagg aaattttaga
     1381 aaataaagaa atctttggtt tagatttata tcaagttggg gtaggaaaga aaactgaaaa
     1441 aatcttttgc gaaatgctag taggtcctca agcagttcgt aaaacaatca ataaatatat
     1501 aacagaagtt ggaggaaagt aactatggaa atgactttta gatggtatgg ggcaaatgaa
     1561 gaaaaaatct cactacaaga aattcgtcag atcccagcaa tgaaaggaat tgttggaaca
     1621 ttaatggata taccagccgg agaggtttgg ccgagagaac gaattaaatc tttaaaagat
     1681 gaagtcgaag ccaatggact taccttgaaa gttattgaga gtgttaatgt tcatgatgat
     1741 atcaaaattg gtttaccaac acgagatcaa tacattgaga attataaaca gacgattcgc
     1801 aatttggcag aatttgggat tgaggttatc tgttataatt ttatgccagt atttgattgg
     1861 gtaaaatcgg atttggatta tcgtttagat gatgggtctt ctacgttagc ctatatttca
     1921 aaaaatatcc ctactgatcc ggaggaaatt attagagaag tagaaaattc tactaataat
     1981 tttgaattgc cgggttggga acctcatcgg ttatcacaaa ttaagcaatt attagaagcc
     2041 tataaagaag ttgatgaaga gaaattacga gagaactttg catattttct caaagcaatt
     2101 atcccgacat gtgaagaagt tgggattaaa atggcagttc acccagatga tccaccatat
     2161 tccattttcg gattacctcg tattgttaaa aatcgtgacg atttagattg gttgtgtaat
     2221 gtagtggatt ctccttctaa tggtattaca ttatgtactg gaagtattgc agaacgacca
     2281 gataatgatg tctatgaaat tctatctgaa ttttgtcaac gggatcggat tccttttgca
     2341 catgttcgta atatcaaata tttaagtgac aatggactcg atttttacga agcgccgcat
     2401 aaatcagaat atggttctct agatatgtat aagatattaa aaacaatgta cgataatgga
     2461 tttaatggct atattcgtcc agatcatggt cggatgattt ggggagaaac cggtagacct
     2521 ggttatggat tatatgaccg agctctaggt gcagtttatt taaatggtat ttgggaagcg
     2581 cttgaaaagg gggcgagtaa ataattatat aagggagttt atttagagga aaaattttct
     2641 cttctaaaaa ctaacggaag gaaaggagtg tttaaatatg tttttagata agaacttttt
     2701 attagagaat gaaatagcta aaagactttt tcataaattt gcaaaacacc aaccaattat
     2761 cgactatcat tgtcatttag acccagcata tttgctagaa aataagcaat acaagaattt
     2821 gactcaggta tggttaaatg ataaaggagc tggagatcat tataaatggc gattgatgcg
     2881 agctaatggt atagaagaag agttgatcag tggtccaggt gatgattatg aaaaattttt
     2941 agcttttgta aaaacagtgg agaaggcgcc aggtaatcct atttttgaat ggtctcattt
     3001 ggaattacaa cgttactttg atattgattt gacaatttct gagaagaatg caccagaaat
     3061 ttggcgacgt gctaacgaaa aaattcaaac ggattcctat cgcccgaaag aattgttgga
     3121 gatgatggat gtgaaagcca tctgtacaac ggatgatcct acagatgact tgtggtatca
     3181 tactgagctt tctaaacagg ataattcttt caaggtatta cctactttcc gtccagataa
     3241 ggtctggagt atcaaatcat caggcttttc gaactatatt ttaaaactta gtcaaacagc
     3301 ggctatacca atcaaagact ttaatgggtt gaagcaagca ttgagccaac gagtggatca
     3361 ttttcatcaa attggctgtc gattagcaga tcacggtttg aatacttatt tttatgaaat
     3421 agcagatgaa aaaatactcg acaatctgtt aataaaagct ttagcaggaa aaacggattt
     3481 ccatttatca gaaattgcac aatttgttac aggtattcaa ttacatttga tgtctgagta
     3541 tacaagatat ggttggacga tgcaaatgca tttaaatgct ttaagaaata attcaagtat
     3601 attaaaggaa caaattggag tagatgtcgg tggtgattcg atgggggatc aagctagttt
     3661 gacacaaaat gttgtagcat tgttcgatac cgctgaaaag agtgggtgct taccaaaaac
     3721 aattttatat tcattaaatc caactgattg gcttccacta gcaacagcaa tgcaaagctt
     3781 ccaagggggt atgattcaaa aaatccaatt gggatgtgct tggtggttca atgatacagc
     3841 tgaaggaatg cgtcaacagt tgactacgat ggcacaacaa agtttgttgt cgaattttac
     3901 aggtatgttg actgattctc gaagttttct aagctatcct cgccatgagt attttcgtcg
     3961 agtattatgt caattactaa gcgagtgggt taaagcaggc cgaattccag atgatgaaat
     4021 gtatcttagt cagatagtgg agaatatttg ttatcgtaat gcgtataatt attttaattt
     4081 ttttgaaatc ggaagtagaa aataatactt gaaataaaag cttaaattta ttctttgtaa
     4141 gttagtacag ctagacaaag tatgtatcca taaaggaagg agatagatat gaaaaaagta
     4201 gaaattttaa gtaagttaga agcggctgga gtcattgcgg ttgtacgtgg gaaaaataag
     4261 gatgaggcca ttaaagcaat aaattcaatt attgcgggtg gaattaaagg gattgaacta
     4321 acttttacag ttcctaatgc tgaagaaata atcaaagaaa ttgctaaaaa atatgctgat
     4381 caaaaagatg taataatcgg agccggtacg gttttagatg cgtttactgc tcgtatcgca
     4441 attatggctg gggcagaata tatcgttagt ccatcttttg atcgacaaac agctgagatt
     4501 tgtaatttat atcaaattcc ttatttacca ggttgtatga caattactga gataaaggag
     4561 gcacttactg ctggcgtaga tatcattaaa ctattcccag gttctgcttt tggtccatcc
     4621 attgtaaaag cctatcaagc tccattacca cacgtaaata taatgcctac tggcggagta
     4681 agtatagaga acttagaaga ttggtttgaa gtaggagttg ttgcagttgg tgtaggtggg
     4741 aatttattgg cttctgttga aaaaggtgat tttgaagaag taagtc
//
LOCUS       DANKAY010000160         4757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000160 DANKAY010000000
VERSION     DANKAY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="PWY98_002799"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="PWY98_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2038485.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="PWY98_002800"
     CDS             724..1878
                     /locus_tag="PWY98_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2038486.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="PWY98_002801"
     CDS             2093..3061
                     /locus_tag="PWY98_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2038487.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="PWY98_002802"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="PWY98_002802"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2038488.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="PWY98_002803"
     CDS             4091..4699
                     /locus_tag="PWY98_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL2038489.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DANKAY010000161         4694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000161 DANKAY010000000
VERSION     DANKAY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            3..422
                     /gene="gpsB"
                     /locus_tag="PWY98_002804"
     CDS             3..422
                     /gene="gpsB"
                     /locus_tag="PWY98_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2038490.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            444..811
                     /gene="rnpB"
                     /locus_tag="PWY98_002805"
     ncRNA           444..811
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWY98_002805"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            1020..2186
                     /locus_tag="PWY98_002806"
     CDS             1020..2186
                     /locus_tag="PWY98_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2038491.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            2232..3728
                     /locus_tag="PWY98_002807"
     CDS             2232..3728
                     /locus_tag="PWY98_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2038492.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            3809..4492
                     /locus_tag="PWY98_002808"
     CDS             3809..4492
                     /locus_tag="PWY98_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2038493.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         1610 a          831 c         1005 g         1248 t
ORIGIN      
        1 atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa
       61 tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg
      121 aaacttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag
      181 tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac
      241 caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg
      301 aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc
      361 caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt
      421 aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga
      481 ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata
      541 agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa
      601 gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg
      661 taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa
      721 caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg
      781 aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg
      841 acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc
      901 agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt
      961 aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta
     1021 tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg
     1081 ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt
     1141 ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag
     1201 taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa
     1261 aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat
     1321 cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg
     1381 ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg
     1441 cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga
     1501 caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag
     1561 aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg
     1621 atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg
     1681 caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca
     1741 tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta
     1801 ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg
     1861 gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag
     1921 aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg
     1981 ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat
     2041 atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa
     2101 gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc
     2161 ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg
     2221 aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac
     2281 aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg
     2341 cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc
     2401 caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg
     2461 gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag
     2521 atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta
     2581 gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa
     2641 aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc
     2701 aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag
     2761 gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt
     2821 taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac
     2881 ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa
     2941 ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg
     3001 tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat
     3061 atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt
     3121 ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc
     3181 aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga
     3241 accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata
     3301 tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac
     3361 tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg
     3421 tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt
     3481 atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg
     3541 tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata
     3601 tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac
     3661 cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg
     3721 taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag
     3781 ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag
     3841 tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt
     3901 caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc
     3961 agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa
     4021 gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat
     4081 tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc
     4141 tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat
     4201 tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta
     4261 tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt
     4321 ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc
     4381 caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat
     4441 tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt
     4501 gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg
     4561 aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt
     4621 tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga
     4681 gaacaccgtt tatt
//
LOCUS       DANKAY010000162         4685 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000162 DANKAY010000000
VERSION     DANKAY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..247)
                     /locus_tag="PWY98_002809"
     CDS             complement(<1..247)
                     /locus_tag="PWY98_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038494.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLN"
     gene            815..2050
                     /locus_tag="PWY98_002810"
     CDS             815..2050
                     /locus_tag="PWY98_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2038495.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(3049..3309)
                     /locus_tag="PWY98_002811"
     CDS             complement(3049..3309)
                     /locus_tag="PWY98_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2038496.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(3336..4637)
                     /locus_tag="PWY98_002812"
     CDS             complement(3336..4637)
                     /locus_tag="PWY98_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2038497.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         1589 a          957 c          730 g         1409 t
ORIGIN      
        1 aattcaatga ttgaatctca tttatttttt tcctgatttt gtcttcttct gtttctacta
       61 atatatcgga gatctcgaca ttcagctttt tgcaaagcgc acttaagata ttgatgcctg
      121 gcgacatatt cttgttttcg atattgctga cagttgcttg ttcacaaatg ccttctgcta
      181 attgtacttg tgttaacccc tttttctttc ggtattcttt tagtaatgct ccatctactt
      241 taatcaaaac aatttccttc cctttaaaaa attatttctc ctttatatca tacacaaaaa
      301 aaagttctaa acaaccaaaa atgtttagaa cagaataaaa attttaattt taaaataata
      361 ttcaaattaa acaactggcg tatctgctcc ttgatttctt tttgtgttat ctgaataatt
      421 atcgtttaca gttataacac atagtgaaaa ttgtcatggc gagaaagtct aagaatattt
      481 ttaaacataa ctatttcttt cttacgtgtg tataatgacg acagcacacc tatcaattaa
      541 tataattatt ttattttttt atgttctcat gtttattttt aatgacacaa cttctcgtat
      601 ttatctggca caacatataa atcgatcaaa aaattgttgc gtgttttttt gaaaattgga
      661 gtttcaactt gattacaaaa tttaaattta gaagttgtga aaacagcgag agacagttta
      721 atattcaaaa tatacatagg ctactcgaac gctgtgtttt ttagtatact agtaagaaac
      781 gaagggatag actcattcca ataaggagga tttcatgaag acaattcttt ttgctcctgc
      841 agtattcaat ttagctgaga caacacgaat gattgaggtt gccaaagcta tgaaagaccg
      901 ttattactgt gtattctttg gcttttctaa aacttttgct cctttgatca cagaaagtgg
      961 ctttgagtat catttactag cgccgatcct tacgaaaaaa caagaagaac aaatcataaa
     1021 atttgaccag ttgcgtacac ttaaaaaccc ttttaccaaa gaaatggttg cctctcgtat
     1081 cactagtgaa cagagtctga ttgctaagtt tgagcctcga ctgattgtga caggttcgaa
     1141 tgtgacaatt tttttatcgg cccgcaataa aaagattcct ttagtatatg ttaaacccta
     1201 tgctttgagt gaaccttttt ttcaatcaga gcatgcgctg atgccccacg agttacgttt
     1261 cttcggccct ttaagcaatc ccctttggca ctggacaaaa aagcgtatag taaatacccg
     1321 atggatcccc aatgccttca aacacgctgc acaaaaagaa atgcttcctt taccaaaaac
     1381 aagtttacaa atgatggatg ctgatctaaa tctcatcaca acgcctaaac tatttttgga
     1441 agaagaacag ctgccggaaa attatcgaat cgttggtcca atttttgcta agctcagtac
     1501 ttccttgcct gaagaagtgc taacttttat acagcgaaag caacaagaaa gaaaacgtat
     1561 tatctatttc gcaatgggta gttcaggcaa tccgcgtatg ttacgcaaaa ttttgttctt
     1621 cttacaaaaa caaccggatc tagcggttat tgcacctatt gactatctgc ttgatgacat
     1681 tcaatctgac tcagaagatc ttttaataac agaatatctg tctagtcatc ttttgcatga
     1741 attcattgat ttttctttta tccacggcgg ggaagggacc gtccaaactg cctgtgaatc
     1801 cggcaaaccg tttatcggtg tggggatgca atttgagcaa agctgtaata ttgactattg
     1861 tgtaaaatac ggaaacgcaa tcgccctcac aaaaccagtg actgaaagaa aactgcagat
     1921 tgctctgcgc gaagtttctt cacccaagat tcgtcagcag gcacgcacgc tgcaagccgt
     1981 ttttccaata aatggtccac aacaagcaat tcaagaaatc gaaaccttcc tgagcaacca
     2041 cccaatatag tttcaagtag aaaaagcttt tttaatagag actcatcagt aatttcgcta
     2101 ttcgagaccc agacagtgaa aatacaaaaa aagtaaatag tggattgaaa agttagagtt
     2161 acttaaatta cggtgtctaa aaaacacaaa atcatcctta aaataaaaga tctgacgcta
     2221 tccaaatgag cataggtagc gtctgccatt tattcttagt caaattcttt gacacctttc
     2281 tctggttgga tcatctcaac gtttcttcaa aagaaagacc ataataatta ggcaaataca
     2341 ttttcttaaa atgtgtcata tagatctaga agcatcgctt tgtagagatt tgtgccgcta
     2401 gtcccctcaa caattactaa atcaaatgta ggatttacca ctttatcttt gataatcaac
     2461 ctccctcttc tgttgagaca atgatctcgc aatccgatgc atactcctat ataaactgcc
     2521 agcaatcccc acatattgag tagatcatgt cagaatgttc tatgtcgtca gcaacgatac
     2581 actaaaagtc acgttcacca tgactgattg ctattcccag cacaattatt tctatacacg
     2641 tttcatgatt tgaataaaca ctcacatcat acatattacc attcatacac catactgcag
     2701 cccctactat atggttgtaa gctcccaaat ctatatttga cttgatcact ttggtagtag
     2761 atctactaat tctaaatcca ttgctgataa aatttccatt ttttcctcct tctttctgcc
     2821 aaaacagtta aaacaataat aatctttatg taaaaaaggg ctgtgacaaa agccttgtca
     2881 cagtcccata ttccgaataa acggtggtca aaagcttgca tctgcggtaa taagttcaac
     2941 caaacgaaaa ataagccaac atttcacaaa aatttaagaa gcaattttcg taaatttctt
     3001 cttatttctt gaagctaacc acttctgtta caaccacttt tagaacattt aaagcttcga
     3061 tgtgacaaac catctatgct gctcgatcat aaaattgata gcaaaaattc cacaaagtac
     3121 atatgtcccc catgaaaaca aaacattcat atgcggtaca atgatctttg gaagaatcac
     3181 gatcaaaaat gatgccagtg cccaaatcca tcgattgatt tgaatatgat atgtagctag
     3241 catattcaat ataaggtgac tagccgttaa taaaaagata aaaacaaaaa ataaaatcag
     3301 ccaatacatt gtttcccacc ttctaaaaac caaacttaga cagtctggac ttcgttttct
     3361 ccacgacttt gttcatctga cagcatcttt ttatcataag cttttacaaa tggataatag
     3421 ataataaatg ctatcaatgt gttgatcatg atcaagataa caacacgcca gtcatttcct
     3481 gctgaaagat aaccgccgat tggagctggt aatgtccatg ttggcaaaac agtaaatcta
     3541 ttgaccattt ctaatttgac tgcaaaataa ctgataatag tcaaaactaa aggaactaag
     3601 ttgaacggga tgaacatgta aggattcaac atcataggag ctccaaaaat tatgggctca
     3661 ttgatattga agataccagg aataatagaa aaacgaccaa cctctttcaa gaattttgat
     3721 ttacagaaga atataaacaa aacagatacc acgatcgttg ctcctgcacc accaattgtt
     3781 accgtccatt gataaaactg ttcaggagcg atatagggta attgggtggc agctgtacca
     3841 tctgccaatg cctttgcatt ctcatccaac atgatcagcc acattggacg aacaacagcc
     3901 cctacaatac tcatgccatg gataccaaat gaccacaaca aatggatcaa aaacattggt
     3961 agtaaaacac caagcaaatt attccctaaa atattggtta aaggctcgaa taacgataat
     4021 aaggcagcgt ttacatcaaa gcccgcgacg cttcgaatca gccaagtaat tgttaaaatg
     4081 actgcacctg gaatcaatgc ctcaaaacta cgggcaacag caggtggcac ttgttctggc
     4141 attttgatag taatatttcg atttttaaag aaacgatata cttccactgc aaaaatcata
     4201 gataagatcc ccgcaaacat cccagatgct cccaaagaac tcataggtaa gacccagcca
     4261 agaggtgcct cttcaggagt taaaacgttt acaggaactt gtaacataaa gaacgttcct
     4321 aaagataata taccacctgt cacactatct aatccatatg atttagctag atccgaaccc
     4381 ataacaaaag ttgcataaat agccatcaat cccatcgtca tcctaaaggg taaaacaatc
     4441 gttgccgaat aaggggcaat aaattcattc catccaggaa acggtaaatt gataaagacc
     4501 ataaaaaatg tcccaatcaa aatcaatgac aatgttgaaa caaccccatt acgaattgct
     4561 aataaatgac gctgtgtacc aatttttgcc attggtggga caagtttctc ttcgacccat
     4621 ttcatcagct tttccactaa aaccactcct tttcaataaa atatgaacca ttcattgcat
     4681 gacaa
//
LOCUS       DANKAY010000163         4618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000163 DANKAY010000000
VERSION     DANKAY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..288
                     /locus_tag="PWY98_002813"
     CDS             <1..288
                     /locus_tag="PWY98_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038498.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="PWY98_002814"
     CDS             347..787
                     /locus_tag="PWY98_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038499.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="PWY98_002815"
     CDS             799..1467
                     /locus_tag="PWY98_002815"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2038500.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="PWY98_002816"
     CDS             1531..2154
                     /locus_tag="PWY98_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2038501.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="PWY98_002817"
     CDS             2210..2596
                     /locus_tag="PWY98_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2038502.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="PWY98_002818"
     CDS             2809..4200
                     /locus_tag="PWY98_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2038503.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1523 a          776 c          982 g         1337 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttat
//
LOCUS       DANKAY010000164         4560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000164 DANKAY010000000
VERSION     DANKAY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..792
                     /locus_tag="PWY98_002819"
     CDS             <1..792
                     /locus_tag="PWY98_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2038504.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="PWY98_002820"
     CDS             889..3138
                     /locus_tag="PWY98_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2038505.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..4316
                     /locus_tag="PWY98_002821"
     CDS             3141..4316
                     /locus_tag="PWY98_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2038506.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4319..>4560)
                     /locus_tag="PWY98_002822"
     CDS             complement(4319..>4560)
                     /locus_tag="PWY98_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2038507.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1578 a          659 c          834 g         1489 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct agttaatttt
     4321 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
     4381 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
     4441 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt
     4501 gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta cgtggcggaa
//
LOCUS       DANKAY010000165         4425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000165 DANKAY010000000
VERSION     DANKAY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            690..1658
                     /locus_tag="PWY98_002823"
     CDS             690..1658
                     /locus_tag="PWY98_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2038508.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="PWY98_002824"
     CDS             1669..2484
                     /locus_tag="PWY98_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL2038509.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="PWY98_002825"
     CDS             2552..3262
                     /locus_tag="PWY98_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2038510.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="PWY98_002826"
     CDS             3298..>4425
                     /locus_tag="PWY98_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2038511.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DANKAY010000166         4377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000166 DANKAY010000000
VERSION     DANKAY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            73..234
                     /locus_tag="PWY98_002827"
     CDS             73..234
                     /locus_tag="PWY98_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2038512.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="PWY98_002828"
     CDS             253..1530
                     /locus_tag="PWY98_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038513.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="PWY98_002829"
     CDS             1540..2196
                     /locus_tag="PWY98_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2038514.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="PWY98_002830"
     CDS             2196..3572
                     /locus_tag="PWY98_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2038515.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3858..>4377)
                     /locus_tag="PWY98_002831"
     CDS             complement(3858..>4377)
                     /locus_tag="PWY98_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2038516.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DANKAY010000167         4306 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000167 DANKAY010000000
VERSION     DANKAY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            106..543
                     /locus_tag="PWY98_002832"
     CDS             106..543
                     /locus_tag="PWY98_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038517.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            638..1522
                     /locus_tag="PWY98_002833"
     CDS             638..1522
                     /locus_tag="PWY98_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2038518.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1744..>4306
                     /locus_tag="PWY98_002834"
     CDS             1744..>4306
                     /locus_tag="PWY98_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038519.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1409 a          722 c          963 g         1212 t
ORIGIN      
        1 tattttaaaa atttttttta cttagaatcc agccacccta gatatttttc tgtataatct
       61 taaataaaga aaacgatatt aaaaaatgca cgtagaagta gataaatgga gtggttttgc
      121 aaatttcttt tatttctaga agttattcac gtatacggat ttgtttttct cacaaataaa
      181 aaggcaggaa gtacaatgga tcatttgtat ataaaatata ttcatcaatt ttccgttttg
      241 gtattaggca cactaattat agcggggatt gttgcattta ttattcaagg tcgtgacata
      301 ttaacagtac atcaagagtt tttcagttat gcatttggta ttcctctgat cgctataagg
      361 tctgataaca aaaagtggaa gaaacgctat tacattctct tgctattttt gtgtttttat
      421 ctgccaccta agatttttac tttattaggt atacaatgcc tatcattttc ttttatttcc
      481 tatatcattg ttttaatttt cctcagtttc atagtatggg tattttatct tgtcaaaaaa
      541 taaatgaatg tttcatgaac agtacttcat cacagcctaa tttttttctt tatgctatcg
      601 ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg aaaaaagttt tattagtggt
      661 aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag caattagcga tagcaaaatt
      721 ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa aaagcagggg atgcaaaaaa
      781 ctttactcgc gaagcggcta cggaagggta tcatagtgtg tttgtcatgg gcggagatgg
      841 aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa catcggccaa atttcggatt
      901 tttcccattg ggtacggtga atgacttggc tcgagcatta gggataccgc tagaacctga
      961 ggaagcgatc aatcattttt cgattgaatc agtcaagcca ttggatattg gaaaaataaa
     1021 tgacgactat tttatgaatg tcgtcgcaat tggctctatc cctgaagcaa tcaatgatgt
     1081 agatgcggag aagaagacga aatttggaaa actagcttat ttcatgtcag gaatcaaaca
     1141 gttagcttcc acacaatcct actcgtttca cgtagaagtt gatggaaaga aggaagaaat
     1201 tgagagcagt atcttgctga taggactaac taactcagta ggcggttttg aaacattact
     1261 tccaaatgca aaagtggatg atggtaagct ccatttggtt tatttaaaag attcttcttt
     1321 gatcgatacg cttaaaacat tgccagacct attaaaagga gtagatgaat ctacgaaaaa
     1381 tctagcctat cttacttgtg agcaattagt tgtgtcatta gctgatgagg aagaattgac
     1441 gacgaatgtt gatggagatg aaggggataa attacctgtg acaatcaaag tgttaccctc
     1501 acatttacag gtttattgtt aaaatggagg ttgtgaccga agagttgtca ggaggacatc
     1561 ggttgttcgg aataagggac tgtgacaaga cttttgtcac agcctttttt tgtacaaaaa
     1621 agaaattttc acctaacaga aaaatttgcc ataaaaatac atattttaaa tatgaaagta
     1681 caattacaaa taaattaaaa cgttaaaata aaattgtcaa aacgtcagag aggatggtgg
     1741 agaatgaatc aatatgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg
     1801 atggtgacat ccgtttttgc cgatgtagag aaaacaagcg aatcaacgca atcagagaat
     1861 caaagttttg acaagactac tttttcagaa gaatcaatta cttctgaaac tacagaatcc
     1921 caaagtgagg cgcctacttc taatacaagg caagaagaaa ataagacaag tgagacctca
     1981 gaagtagctg aacaaacgtc tgatagcagt gaagaggtac aggttagagt tgtttcagcg
     2041 gatgggtaca gcgaagctgc cacaacagaa gaactgacgc agcttctcgc agacgaatca
     2101 gtagctaaga ttcgtttaac tcagcctttg actttagaca gggaattgga gatcaagcga
     2161 gatatcgtta tcgattttgg cggacttgct cataatttcg gcacacatca catctatatc
     2221 aatgagacac ctcagtcgat cgagtttcag aattttaaag gaacggcagc tcatccaggt
     2281 gggttgatac caaatatcga tggaaatgcc attatcattg cttatatgtc cgatttttgg
     2341 ggaaacagat atcattttac gggagaaata aaatttaccg ggacacttga cttatatgat
     2401 ggttctaaat taggcctgat ttacgcccct cgtgcaactg tcacattaga tggtgtttcc
     2461 ggtgtattgg atgtccaacc agtgaaagaa gggatgaatg ccgctccggg taaagcttat
     2521 ttttgtagaa gctatcagct aaatgtaatc aatggtagtc agctttatgg accatatcta
     2581 ggaaaattct atggttttct agattcaggt gatgagacgg gcagtggaaa aacagcacca
     2641 ggactccata tcttgagtgg ttcaaaggtc tctcttgact atgatcgggc agatggcgtc
     2701 agcgatgaag gagaggctgt cgatacgcta cccgggaatg tcacatttaa agtcagtggc
     2761 tcaggaagtg aattttcggt aaatacaaag atcaatatta ccaacgacaa caatcgcgga
     2821 attatccaaa tgagaggctc aggatcaaga gtcgctgtgt cgaatgatgg gaaaatcaca
     2881 atcaatacag cgactactgg cggatttcgc ctgcaaggag attctagtca aatccatgta
     2941 tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg gcgaccaacc tggaaacaat
     3001 gggatgcgtt ttgtagggaa aggactttct ttgatcgtag aaggagccgg atcagtcatc
     3061 gatattgata agcaaagtgg tcgttctagc gcagttcgtt ttgagaacgg tacgcaaaaa
     3121 ctaacagtaa ctaatggagg ctcattgaag gtaagaaatg caggggatgg caaatcttat
     3181 gtgaacagac tcaatcaagg aaaccaagcg atccaatttt atgacgcttc tggttttcta
     3241 gaaagtccag ggtcagctga ttttacggtt actggagaaa aatcaaatat cgatatacgc
     3301 gcagattccg gagctaccgt agatacaact ggagatattg atttgaactt tatggctggc
     3361 gaaaaaactt atttagtcat ggtcgggaaa actggggaag ataagactgg catcatcagt
     3421 ggggatgtgt tgaatgtaga cttagaatct cctacctatt tcgatttcca aaacaaacgg
     3481 atcggaagcg agactaatgg cataggtggc tgggtatttc aaggaaatgg cgattcttct
     3541 ttgaagatcg ctcattcaga aattgcttta tggcgaaagg caggagtaac gttcgataac
     3601 tttgaaaaag atccggatta tttttctgat atgacagact tatcgatcaa tggaaccgac
     3661 ttaggtaatt ttgtatcaag ccctagtgca ggtttaacaa atgaatttgc tcagacaggt
     3721 gccggaatga aaaattataa tcgaatcagt gccaataacc agcttccgat gattgaatcc
     3781 ttaagagtac caacgaatgc agataaaaaa atctatgggc atgtcgttgt tcccgaaggt
     3841 gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag aagtgacttt acgtattact
     3901 tatgcagata cgacgaaatc acctgtagaa attactgctc tcacacaagg acaaacagat
     3961 gaaaatgaag ggacaggata taatccgtgg ggagaaggcg aacaaggcgg tttgtttgag
     4021 gttcaagtac ccaacgaaga aagactaaca acgggggatc aagtgcaaat agttgctgct
     4081 aaaaaagtaa gaggcggcca agaagtgaca gaactggact ctgccaaaac gacagtcgat
     4141 gtgattcctc ctgagcctgc taaattaagt accacattga tcaatgcagc ttctcgctca
     4201 atcacaggta caggcgaagc aggagctttg gtttcattga aaaaaaatca agctgttttg
     4261 tccgaaacaa ctattgatga tacaggtaaa tttgttcttt ctatcc
//
LOCUS       DANKAY010000168         4282 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000168 DANKAY010000000
VERSION     DANKAY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(37..1257)
                     /locus_tag="PWY98_002835"
     CDS             complement(37..1257)
                     /locus_tag="PWY98_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL2038520.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="PWY98_002836"
     CDS             complement(1277..1888)
                     /locus_tag="PWY98_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2038521.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="PWY98_002837"
     CDS             complement(1931..2863)
                     /locus_tag="PWY98_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2038522.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="PWY98_002838"
     CDS             complement(3284..4144)
                     /locus_tag="PWY98_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038523.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DANKAY010000169         4257 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000169 DANKAY010000000
VERSION     DANKAY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(88..468)
                     /locus_tag="PWY98_002839"
     CDS             complement(88..468)
                     /locus_tag="PWY98_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2038524.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            532..1725
                     /locus_tag="PWY98_002840"
     CDS             532..1725
                     /locus_tag="PWY98_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2038525.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(1802..2386)
                     /locus_tag="PWY98_002841"
     CDS             complement(1802..2386)
                     /locus_tag="PWY98_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2038526.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2587..3585
                     /locus_tag="PWY98_002842"
     CDS             2587..3585
                     /locus_tag="PWY98_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2038527.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            3697..4206
                     /locus_tag="PWY98_002843"
     CDS             3697..4206
                     /locus_tag="PWY98_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2038528.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1341 a          794 c          780 g         1342 t
ORIGIN      
        1 cttttgcttg ccaaaatttc cacctcgatc aacttgttct atggcttaca taaaaattct
       61 agcacatttt cagaaaaaat acacttttta tttcttattt tctttccgtt ctagttcaga
      121 taatgcttct tctatccaaa taggtaactg ctccgcaata gtggtaaatc cgatcttacg
      181 atttttattc gtgaaatacc tttttctgcg atatggaaca gctggatttg ttttttctaa
      241 cgttcttaaa gataaactga tttttttgga atattcatca atgtcgatga tctgaactgt
      301 tactggctgt ccgactttta acaatgaatg gatatttttc gtataaccgg attgtacttc
      361 tgaaacatga ataagtcctt gcgtctcgtt atcaagagaa acgaatgcgc cataaggctg
      421 tatccctgtc acttttcctt caattatttg tccaatttta taagtcatct acttccctct
      481 cttcgcattt atcattatac tgtaaatagc tcaaaaaaag aagcgacgaa aatgactgga
      541 ggtcttaaga tgatcgaatt tgataaacag atttcaagaa aagatacaga aagtgtaaaa
      601 tgggacgcaa ttgctgagac atatcaaatg gatgacttac ttcctttgtg ggtagcagat
      661 atggacttct tgtctcccgg tggggtagct aaagcgttta gtgaatacat caaacatgga
      721 atctttggtt atgccactct gtctgataaa ctatatcagg ctatcattca ttgggaacgc
      781 caacaccatg ctgtggagtt gacgaaagaa aatatcgttt ttacaagtgg tgtgttaacc
      841 agtttagctg cagcagtaca aacttttaca aaaccaggcg attctatttt gattcatgac
      901 ccagtttatc ctccattttc ctctattata gaaacaaatc aacgtcacat cgttcgaagc
      961 tctttgctag aaaaaaataa tcatttcatc atggacttgt ctgacatgga gaaaaaaatc
     1021 gaagaaaaca atgtaaaagc aatgatttta tgcaatcctc acaatcctgg agggcgtgtt
     1081 tggagcaaag aagaattgaa acagttaagc gaattgtgtt taaaacatca agtgattctg
     1141 tttagtgacg agatccatca agatttaact ttattcgatc atacatttac ttccatgcta
     1201 acgattgatc ctaatttaga tgatctacta gttgctttta cttccgcaac caagactttc
     1261 aatctagctg caatcaaaaa ttcaatggta tttatcaaaa atccagagat gaaagctgat
     1321 tttgaaaaac atttagtgat gaaccaacag cacgaaatca acacttttgg actgattgga
     1381 acccaagcgg cttatgagac gggtggagaa tggttaagcc aactgattcc ttatctagaa
     1441 aaaaatgttc aaacagttaa tcagtttttt gaacagtatc ttccgaaagt gagaataatg
     1501 aaccctgaag gaacttactt aatgtggtta gattttaccg cctatatttc agacgaccaa
     1561 gaattggaga acactcttgt tcggaaagga aaagtcgtat taaatcctgg catcacgttt
     1621 ggtccttccg gtcatgggca tatgcgcttg aatattgctt gtccagaaga aactttactg
     1681 gaaggactta atcgaatcaa gtatgcttta gagacaatcg attaaatcat ttattcaaaa
     1741 taaaaggacg agaccttact aggtttcgtc ctttttcata attactccgt accataccaa
     1801 actattctga aatttcaatt gtttcaatca ctacatcatg tacaggtcgg tcttgtggac
     1861 cacgttgtac atttgcgatt tcatctactg tgtccatccc ttctagtaca tgtccaaaga
     1921 ctgtatgtcg gaaatctagc caaggagttc cgccaccttt atacgcttca atgatttctg
     1981 atgggaatcc agcaccttct agttgtccaa gcatattagc tggcacattt gtgttattta
     2041 caataaagaa ttggcttccg tttgtgtttg gacctgaatt tgccatagat aaagctccgc
     2101 gaaggttgaa cagttcacga ctgaattcat cttcaaaact ttcgccataa atactttcgc
     2161 cacccattcc tgttccagtt ggatcgccgc cttggatcat gaaatcagga ataacgcggt
     2221 gaaaaatcac gccatcataa taaccttttt tggaaagttc aatgaaattt tgaaccgtct
     2281 tcggtgctag ttcagggaat aattgaacag taatgtcacc acggtttgtt ttgataacag
     2341 cttttggtcc tttttcactt tttaaatcta gttgtgggaa tgccatattg tttcctccta
     2401 atatttgcat acttcataat aataataaca taatacctaa cctctcgtaa aaatactaga
     2461 tttgttttct tttatttttc atcactagat acattctata acttaccgtt tcgatttcga
     2521 attcaaacat ttttgagaag cattctcaat taaattctgc tatagtgtga aaagggagga
     2581 atttttatgg aaatctatga cattacaatc atcggtgcag gtcctgtagg gatgtttgcc
     2641 gctttttacg caggaatgcg ccaagctaaa acaaaaatca ttgacagtct acctcaactt
     2701 ggtggtcaat tagcaacttt gtatcccgaa aaatatattt acgatattcc tgggtatcca
     2761 gcaataaaag caagtgaatt aatcgatcaa ttagaaaaac aattaactac ttttaaccat
     2821 acattccatt taaaagaaga agttctctcg cttactcgtg aagacgaagt tatcgagatt
     2881 actacaaata aaggcatcca ttattccaaa gcagtcattt tagctttagg aaatggttca
     2941 ttccagccaa gaaaattgaa tttagataat gctgaatcat ttgaaaacca cggattggat
     3001 tatttcgtga atgatttgat gaagtatgct ggaaaaaaag tcgctattgc tggaggtggt
     3061 gattctgcta tcgattggac gttgatgtta gagcctattg caagcgaagt ttacttgatt
     3121 catcgtcgcc cggaatttcg tgcgcacgaa catagtgtat cacgcttgaa atcttcatct
     3181 gtcaacttgc ttactcccta tctcatcgat ggattatcag gcaataatgg ggaactgaca
     3241 gatatccggt taaaaaaagt gaaaagtgac gagacgattg atttgatgat tgattctctg
     3301 atcgtcaatt atggattctc ttctaattta gaacatcttt cttcttgggg acttgatagt
     3361 accagaaatg ccattactgt aaaatcagat atgtctactt ctattccagg cgtttatgca
     3421 gctggtgaca tctgtaccta tgaaggaaaa gtgaaactga ttgctactgg gctcggtgaa
     3481 gcacctactg cagtgaacaa cgctttacac tatattaatc caaaggaacg aactcagcct
     3541 ggtcacagca caagcttata cgataaaaat cttcgtccta gttgattttt tatatctctt
     3601 tgcatttcaa gttaaacccg aaagcagttt cttgcatttt tttcatgttc cgttaacatg
     3661 aaatagagaa agaaaaaata aagggggagc aataatatgg tcgtcaaaac tgagacttta
     3721 gaagaaaagg caagagaact attgaaggaa cgtggcgtaa ctgtagaaga tatcgcacaa
     3781 cttgtcatgt ttttacaaaa agactatatt tctgatttga ctttggcgga ttgtactgaa
     3841 agtgtcaaca gcgtgctaac aaagcgtgaa gtacataacg ccattatcac tggaatccaa
     3901 ttagatatat tagcagaaaa gaaaaaattg atgagtccgt tgcaacatat tatcgaagat
     3961 gacgaaggcc tttacggtat cgatgagatc ctagccttgt caatcgtgaa tgtttacggt
     4021 acgatcggct ttacgaatta tggttatatc gacaaggtga aacctggtat tttaaaagaa
     4081 ctaaacagcc acgatggcgt tcatgtccat accttcttag atgatatcgt cggagcgatc
     4141 gccgctgctg ctgccagtcg attagcacac aaagaaccag aaaaaagaag tcgccttact
     4201 caataattca acaaaaaaag aaagaaaacg tctatttaga cgagttcttt ctttttt
//
LOCUS       DANKAY010000170         4122 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000170 DANKAY010000000
VERSION     DANKAY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..98
                     /locus_tag="PWY98_002844"
     CDS             <1..98
                     /locus_tag="PWY98_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2038529.1"
                     /translation="SVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(160..1971)
                     /locus_tag="PWY98_002845"
     CDS             complement(160..1971)
                     /locus_tag="PWY98_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2038530.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1968..2333)
                     /locus_tag="PWY98_002846"
     CDS             complement(1968..2333)
                     /locus_tag="PWY98_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2038531.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2496..2891)
                     /locus_tag="PWY98_002847"
     CDS             complement(2496..2891)
                     /locus_tag="PWY98_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2038532.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2909..3871)
                     /locus_tag="PWY98_002848"
     CDS             complement(2909..3871)
                     /locus_tag="PWY98_002848"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2038533.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3871..4083)
                     /locus_tag="PWY98_002849"
     CDS             complement(3871..4083)
                     /locus_tag="PWY98_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2038534.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
BASE COUNT         1170 a          918 c          671 g         1363 t
ORIGIN      
        1 cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa
       61 accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg
      121 aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag
      181 ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc
      241 tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt cagactggat
      301 ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg
      361 ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt
      421 ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa
      481 atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg
      541 aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc
      601 tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc
      661 ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat
      721 tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc
      781 tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc
      841 attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc
      901 tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt
      961 ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc
     1021 ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc
     1081 caggttttca tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct
     1141 tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc
     1201 tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt
     1261 aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt
     1321 aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac
     1381 atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg
     1441 accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata
     1501 accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg
     1561 aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt
     1621 agattgctga tctttttcca gacgattgat ttcttctata attagttgct tatttttggt
     1681 taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc
     1741 ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga
     1801 aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc
     1861 attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct
     1921 ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc
     1981 tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca
     2041 taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc
     2101 ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt
     2161 tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac
     2221 agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt
     2281 ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt
     2341 tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa
     2401 atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg
     2461 attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt
     2521 ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt
     2581 aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata gacaggctgg
     2641 tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct
     2701 aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc
     2761 attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt
     2821 agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat attaattacc
     2881 cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac
     2941 caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt
     3001 gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg
     3061 ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc aagtttccgc
     3121 aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt
     3181 cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct agcattgctt
     3241 tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt accacacctg
     3301 tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag
     3361 gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga atcaaatttc
     3421 cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca acgtctggat
     3481 cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg tcattatcga
     3541 tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc
     3601 agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac
     3661 ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa cgatgattga
     3721 ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc
     3781 tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac
     3841 ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc
     3901 aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa
     3961 acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc
     4021 acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc
     4081 catctttaag cacttccttc atattataca agtaaaccat at
//
LOCUS       DANKAY010000171         4065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000171 DANKAY010000000
VERSION     DANKAY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(64..606)
                     /locus_tag="PWY98_002850"
     CDS             complement(64..606)
                     /locus_tag="PWY98_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2038535.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="PWY98_002851"
     CDS             741..1130
                     /locus_tag="PWY98_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038536.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="PWY98_002852"
     CDS             1134..1898
                     /locus_tag="PWY98_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2038537.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="PWY98_002853"
     CDS             2027..2974
                     /locus_tag="PWY98_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2038538.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="PWY98_002854"
     CDS             3104..3844
                     /locus_tag="PWY98_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038539.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1355 a          687 c          817 g         1206 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg agtttaagcg
     3961 aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa ctattttcgc
     4021 agatcgtttg caaaaatagt ttattttttt ataaagaaga tttta
//
LOCUS       DANKAY010000172         4044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000172 DANKAY010000000
VERSION     DANKAY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            25..1449
                     /locus_tag="PWY98_002855"
     CDS             25..1449
                     /locus_tag="PWY98_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2038540.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1630..2403)
                     /locus_tag="PWY98_002856"
     CDS             complement(1630..2403)
                     /locus_tag="PWY98_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2038541.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2400..3305)
                     /locus_tag="PWY98_002857"
     CDS             complement(2400..3305)
                     /locus_tag="PWY98_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2038542.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3459..3779)
                     /locus_tag="PWY98_002858"
     CDS             complement(3459..3779)
                     /locus_tag="PWY98_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2038543.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1210 a          749 c          813 g         1272 t
ORIGIN      
        1 ggttttattt tggaggatgc ttacatggat acagagcaac agaaaaaagg aattggttta
       61 attccacttg ctgccttagt aattggttca gcgattggag gtggcgtatt tggtattatg
      121 acggacatct ccggatcggc tggcggccca gctattttca gttgggtact cgtaggaaca
      181 gcaatgttga tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa
      241 ggcgggatct ttagttacgc agaagaaggt tttggccgtt tcgctggttt tatcagcggt
      301 tggggttact ggctcacatg ctggttaggc aacgtagctt ttgcaacatt gttgatgagc
      361 gcattaggat atttcttccc agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg
      421 agtacagtct ttttatgggg atatgcatgg ctagtgaatc ggggagtcga aaatgctagt
      481 ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat tatttttatt tattgtagta
      541 gctatcagca cgttttcgat ccaacaattc attgataact tttctagcga cattatattg
      601 aatgcgaatg gacagccgat cccgttttcc agtcaagtat tgagttgcat catggtgatg
      661 ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa
      721 tcagatgttg ggaaagcaac gattctaggg atattcggct tgattattat ttacgtcttg
      781 gtttccatgc ttccatatgg gacaatgacg atgaatcagc tgcaaacgat caaggcgcag
      841 cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac
      901 ggcggtttga tcatttcttt agttggtgtt tggctctcat ggacgatctt accgattgaa
      961 acaatgggaa acatggctga agacggactt ttgcctaaag catggggaaa actcaacaaa
     1021 aatggggcac caacttatgc tatcgtattg acgacgttat gtacgaatgt ctttttactt
     1081 tcattattat tcactgacag tgcttataac tttgcttaca cacttggtac agcagctatt
     1141 ttcttcacgt ggcttttctt aggattgtac caaatgaaac tttcttatca gcggaaagaa
     1201 tgggtccaat ttttagtagg gcttttagca agtgcctttc aagtatgggc tatgctagtc
     1261 gtagccttta atgaagtaat gcttgttctt gttcttttct tgccaggcat ttatttttac
     1321 ctcaaagcac gtaaggagca aaacgagcct gtacaacata tccacttaga taagatttta
     1381 cttataggtg tgacgatggt cggagtgatt gcaatggttt tatttgccac tgggatgctt
     1441 cacgtataga agtataaaag aagtataaag agaggttgtg agcctgtcgt ttattcaaaa
     1501 acatactagg aaaggagctg tgacacaaat gttgtcacag ctcctttttc agaataaacg
     1561 gtgtttaaga gacagccaat cggcaataag gctgaacgtc tctttcacag cctctttttt
     1621 gatgagtcat catacatttt ttcgcttaaa tacgagatat cctaatgcta agaacaaaac
     1681 gatgtaagct aaattaccga atagcatttg attagtagaa agatgagtca attgcttcat
     1741 gatctcttcg tttccaattt ggttttgcag attcaacata ctgatcggat tccatttgat
     1801 ccactcccat ttttggatga gtgcaataag gataccagat atgattgacg aagcaaaata
     1861 aaagacgatt cctgctgaga tggatgcgcc ggaactattg ataaagcaag caatcatcaa
     1921 cactaaactt aaaatcagcc ataaaccgat ataactaccc aaagtataag tgaacaagct
     1981 ttggatcagt gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat
     2041 cagtttcatc aacacagtga caataatagc taaaaggtat aaataaacag aataaataac
     2101 taatgtgatc catttgctta ctaatatttc tcctcgagaa tactttcgat aaaggagatt
     2161 tttcaatgta ccatattgtg cttccatcga aatgatcgtg cttgcagctg caatcatgat
     2221 aaagacaatc caagatgtgg cactaaaaag ctgagcagtc atttcaactg gatcaatgat
     2281 cgtcgtgtat tttttggcta gaagtgctgt tcctatgagc aaaacaacta atagaacaga
     2341 taaaacagca gttgactttt tcttgatcaa tttaaataat tcttgtttta caagcaatgt
     2401 catttttctt cttccccctt tactgaatcc agaatttcca atagagaaga ttccaaatca
     2461 ttcgtcagat gctggacgtc tgtcacatgg atattttgtg cagacaatag ctggatcacg
     2521 gttcctaata attcttcgtt ttcttttaaa agcttgattt tatcaggatc ttccaaagta
     2581 tatccagctt ctgtcaagat cgttcgagca gctagatcat ttgttgtaga gataacgatg
     2641 aaatcttgcg ctgtttggtt caattcagcc attgtacatt gtttgatgat ctttccttga
     2701 ttgatcagga tcagctcatc gaccattttt tcaagctcgc tgagtaagtg actggagatc
     2761 aagaaagagg tgccctgagt agccaagtcg gtaatgattt ttcgcacatc tcttgttgct
     2821 tgtggatcca aaccgttcat cggctcatct aaaataacga tagacggatg gttcagtaag
     2881 gcaagagcaa ttcccaattt ttgtttcatt cccaacgagt aggatttcgc ttttttgtga
     2941 atgtactttt ccatcttgag ctgattcacg atagtgtcga tagcagatac atcgttggtt
     3001 tcagagaata acttcagatg atcatagcct gttaagaatg gatagatacc cgggtattcg
     3061 atcaatgctc ctacttgttc caacccttta tgactagagg gagaaatctc attttcacca
     3121 atacagattg ttccatctga gtaatggatc agtcctaaaa tagctttcat gatcgttgtt
     3181 tttccagcgc cattaggacc tactaatcca acgattttgc caggggataa gctaaaactg
     3241 acatcgctga gggcttgttt gtgaccaaag ttcttggata attcttggac cgaaagactt
     3301 ttcaaaaata ttcactcctt ttctatagat agtacaagca tacacctgat gagtggagaa
     3361 gtcgctaggt cattggactt atttcttatt ttttcataaa aaaattgaat attaaataaa
     3421 aaggcttatt ttttctgatc agtacttatt ccaagtgtct agtggccgct agtaatcttg
     3481 atgccaatga tactgatgag tagcatcccc acaaagaacc aagtggaaag attcagctta
     3541 tcatgaaaca agaccacacc aattatgacc gaaccaactg cgccaatccc tgtccagata
     3601 ggataagaga gactcatcgg caaacttttg attgcctttg acaaaaagta aaaactagca
     3661 atcatgccaa tcactgtgta aaaagaataa ttgattttag aaaaaccatc actccatttc
     3721 atcatcgttg accagaatac ttccaaaatt cctgctatga ttaattccat ccatgccata
     3781 cttacattcc tccttaacta taaaaaaata cacaaaaaga ggaaaatcat cccttcaaag
     3841 acctaatgga atgatcatcc tctttatcct acccggtggc agattgtata tctttttctg
     3901 tagtatagca cacctatcca gatatcggca atctcatact ttttagtaaa gtgtaccaag
     3961 cactgacgga tatatcgtta gcggaaaaga agatgaatgc tttgcaattt catagaaaga
     4021 agttgttatt tattaatcat ttaa
//
LOCUS       DANKAY010000173         4041 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000173 DANKAY010000000
VERSION     DANKAY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(150..1655)
                     /locus_tag="PWY98_002859"
     CDS             complement(150..1655)
                     /locus_tag="PWY98_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2038544.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1652..2338)
                     /locus_tag="PWY98_002860"
     CDS             complement(1652..2338)
                     /locus_tag="PWY98_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2038545.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2531..3952)
                     /gene="gndA"
                     /locus_tag="PWY98_002861"
     CDS             complement(2531..3952)
                     /gene="gndA"
                     /locus_tag="PWY98_002861"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2038546.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1115 a          855 c          691 g         1380 t
ORIGIN      
        1 ctaaaagata cttacattta attaacggat gttattctat ttaattgttc gctagttttt
       61 ttataatgac atccctgaat ttctcacttg cttgtatttt gtaccttatt ctttgttgga
      121 tatataaaaa aacaagtaaa gcacatgctt tacttgtctt ctgcctttgc gataggaata
      181 aagattctaa aaattgtccc ttgccctact acactttctg ctaagatacg tcctttatag
      241 ttttcaacca gttgtttagc gatagacaac cccagaccat ttccaccctt cgttctagct
      301 cttgcattat ccactcgata aaaacgattg aagatcttat taaggtcttc tttgggtatt
      361 ccttcgccaa agtcctgaat cgcgatctca aattcattca gagtggaaga aatagagata
      421 tggacttctt ttcgtgtagt tgaatacttt actgcgttat ccaatatgat aatgattaat
      481 tgttcaaagt gattacgata aatttgcagg gtcacttctt ttgtcaaatc atcatctaaa
      541 gtgatcacat agtctggata taatatcttg aaattattat aaacttgata ggtgacttct
      601 tttcctttag ttgtttcatt cgcataatga acatctactt gttctgctct tgataagtct
      661 agcatctctt gaactaagct tttcatacgg ctgatttctt gcatgctggc gcttaatgat
      721 tcttctaata tctcaggatc atctttcccc caacgattta gcagactaag atgcccttca
      781 ataattgcaa caggtgtcct taactcatga gatacgtctt ccacgaattg ctcttgttgt
      841 tcaatataca aacgcatacg gtcaagcatc tcattgaata tttcagctaa atctgccaat
      901 tcatcatttg tgtcgatttc aggcatgtgg atatctgatt gcggatcttt acgtatagta
      961 tccattgtat ctctgagtac cttgagtggt tttaagaaat aagaagataa aaagaaacct
     1021 aaaatactac ttaagatcaa agaaacaact tctaaaacaa taagagtcaa taatagctta
     1081 tttcgaatat catagaaaga agataattca tagaagcttt gcgcatagcc gatcttttct
     1141 cttgtacctt tagaatatat aggctggata gataaaaagc cagtttttcc ttctagtgta
     1201 acgatcgttg gaactttacg tgtagtctga atcaggtttc gactgttttc atgggtttta
     1261 aaaatcaagt tctcgtccaa atcataaaca taaagatcca ttgatggctg ccctagctca
     1321 gctataaagg aatcgatctt cattaagttt ccttcgacca gtgaagtatg atctacgacc
     1381 tcatctccgt catttgtcgc tttcgtcaga ttacgatacg tatttactaa tgtcagttct
     1441 tcatccgagt tagccaaacg agaagctact tctgaaatcg tacgctctac attgtttctt
     1501 tcttttgcca caatcagatt gatagatgat ttataggtaa tgacagcgaa aattgtaaac
     1561 acaacgaata tgaagaaaga actcgcaaag gcccacttga ttgtcaagga gggccctttc
     1621 agttctcttt tttccgattt tttcggcttt atcacgagcg catcacataa cctgttccgc
     1681 gtacagtttg gatatagctt tcctccccgg gaacgtcaat tttgttgcga agataacgaa
     1741 tatatacgtc cactacgttt gtttcaactt ctgtttcgta tccccacact ttatttagta
     1801 aaacgtcacg tgctaatacc acgttcacgt tttccattaa agtaagcagc aactcgtatt
     1861 ctctttttgt tagttcaatt acttcatttc cgcgacgtac gacacggttt tctttttcga
     1921 tagtcaaatc gcgataagta atagtagttt gtttagccac atttttatcg ccttcaatat
     1981 cgattcgacg caacaatgca cgaagacgtg ctaataattc ttcgatagca aatggtttga
     2041 cgatgtagtc atctgctccg tggtcaagtc cagatacacg atcgatcaca gaatctcttg
     2101 cagtcatcat aatgattggt gtatttttga cttggcgtac acgtctgcag acttctaaac
     2161 cgttcaattc tgggagcatc agatccaaca atatggcatc ccactcatta tttaatgccg
     2221 catctaaacc agttcggccg ttgtagtgga cttctgcgtt atacccttca tgcttcaact
     2281 ctagctcgac aaatcgtgcc aagttctttt catcttcaat gattagaata ttgctcattt
     2341 attattttat cctttctatt ctactggctt tttaaaaact ctatcattat accgataata
     2401 tgcttaaaaa tctatagtca ttttatcatg gatagcaatt ttaaaaaaga gctgaagtct
     2461 actcataaca atcgacttca gctctttcgt cctcttttta ttttctcatt tcctaacagc
     2521 cgaaagacta ttattcttca tggtaccatg agtaatggaa gattccttct ttgtcgacac
     2581 gctcataggt atgtgcgcca aagtaatcgc gttgggcttg gattatattt gcaggcagac
     2641 gttctgctct gtatgaatcg aagtaagcga tggctgatga gaaagtcggt acaggtacgc
     2701 cagcttggac agccaaggca acgacgtctc tgactgattg ctgatatttt ttcgtgattt
     2761 caacgaagta atcatctagc aataaatttt cgatctcagg atttttgtca taggcatccg
     2821 taattttttg caagaatcga gctcggatga tacatccagc gcgccagatt tttgcgattt
     2881 caccaaatgg caagtcccaa tcaaattctt ttgaagcgac acgtaattga gcaaatcctt
     2941 gggcataact catgatttta ctgaaataca acgcttctcg gattttttcg accaattctt
     3001 ttttgtctcc cgtgaactca aaatcgttcg tacgagacaa aatcttgctt gcttgtacgc
     3061 gttcttcttt ataagcagaa atatagcggg caaatacaga ttcagtgatc aatggaagtg
     3121 gcacacctaa gtcaagcgcg ctttggcttg tccatttacc tgttccttta ttcccagctg
     3181 catcaaggat cacatctact actggtttac ctgttccttc atcgtcttta cgtgtcagaa
     3241 tgtcagcagt gatttctatc aaatagctgt ctaattcgcc ttgattccat tctttaaaga
     3301 tatcagccat ttcttctact gaaaggttta aaatattctt cattaagtca taagattcag
     3361 caatcagttg catatctcca tactcaatac cgttgtgtac cattttcaca taatgtccgg
     3421 ctccatttgg gccaatatat gtgacacatg gtgctccgtc ttctgctttt gcagaaattt
     3481 gctctaagat tggtgcaact agcgcatagg cttctttttg tccaccaggc atgattgaag
     3541 gtccttttaa agctccttct tcaccaccgg aaacgcctgt accgataaag ttgatgccag
     3601 agttggctaa ctcttcatta cgacgcatcg tatctttaaa gaatgtattt cctccgtcaa
     3661 tcaaaatatc gcctttatca aggtgaggaa gtaactcttg gattgttgca tctgttgccg
     3721 gtcctgcttt gaccattagc aagatccgac gtggtttctc gatagattct acaaattctt
     3781 cgattgtata tgttgctttg aaatttttat ctgggtgttc ttctactaca gcggttgttt
     3841 tagcccctgt acgattgaat aaagccactg aatagccgcg gctttctatg ttcaaggcta
     3901 gatttttacc cataacggcc ataccgacaa cgccgatttg ttgttttgac atatgaactc
     3961 ctcctacata cgggatttct ccctaatctc ttacattata gcttaacacg gctacattgc
     4021 aaactaaaga acaacatatt t
//
LOCUS       DANKAY010000174         3999 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000174 DANKAY010000000
VERSION     DANKAY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(371..2752)
                     /locus_tag="PWY98_002862"
     CDS             complement(371..2752)
                     /locus_tag="PWY98_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2038547.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2742..3389)
                     /gene="recU"
                     /locus_tag="PWY98_002863"
     CDS             complement(2742..3389)
                     /gene="recU"
                     /locus_tag="PWY98_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2038548.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3453..3992
                     /locus_tag="PWY98_002864"
     CDS             3453..3992
                     /locus_tag="PWY98_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2038549.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
BASE COUNT         1131 a          747 c          831 g         1290 t
ORIGIN      
        1 caaattatga taattaagca tactatcaga taactcaaaa tgaagccttc tttcaaaaaa
       61 ttattcaaaa ccatatattg tattttttat ttgataataa caatgggatt attaagaagt
      121 ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa gtaaatggat
      181 aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta aatgttagat
      241 cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt ggtacatgat
      301 ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg tagacgtggt
      361 ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta tggtcatttg
      421 cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca gatgaagacg
      481 gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg
      541 tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga ctagtagagc
      601 tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca tagctggaag
      661 atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga tctttgtagt
      721 acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta tttgtcacgt
      781 tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt gacgattctg
      841 aagtgacagg cgtcattttt ttattgtaac ctgtccacac ggaaatcgaa taatttggcg
      901 tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt tttgcatact
      961 cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca gcgatttgcg
     1021 cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc atataagctg
     1081 tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt ccgtcttgga
     1141 agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg aaagccgcat
     1201 aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt tcttccgtat
     1261 tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga ttttttaaga
     1321 aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga acattacggg
     1381 ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc cagtttccta
     1441 caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt ccagtagaat
     1501 atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat
     1561 cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc
     1621 cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag gcaacttgca
     1681 tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat agtttctttt
     1741 gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg
     1801 ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc ttagtattgt
     1861 catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca gcttgatcat
     1921 attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta tctcgtcttt
     1981 tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga ggcatcccag
     2041 caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag tacatctcag
     2101 aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg tagtaggtca
     2161 agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct tcttgggcct
     2221 tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg atcaattgct
     2281 gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg gcagaaccga
     2341 tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct accgacacga
     2401 tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc
     2461 ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg gtagcgtcta
     2521 attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg
     2581 ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata aataatcctt
     2641 ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc acactctttt
     2701 taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc attcttgctt
     2761 tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata aggaatccgc
     2821 ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa aggtagtgat
     2881 ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact attcaaataa
     2941 aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt ttgttcgagg
     3001 catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa agatgttttg
     3061 ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc attatagtct
     3121 gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct cttaggatag
     3181 tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa cccgcgggaa
     3241 agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg attgccgaat
     3301 tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt cttataataa
     3361 ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc acattatagc
     3421 aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta atctatgtgt
     3481 ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg
     3541 ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg gaatgggtac
     3601 ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg gaattgaaag
     3661 aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt ggtaataact
     3721 ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat tatgtcgaag
     3781 ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact cgttttatac
     3841 ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga aaaacgaagt
     3901 atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt cggctgatca
     3961 gtatggacga tcttcaaaat tttagtgaat aatcgatat
//
LOCUS       DANKAY010000175         3864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000175 DANKAY010000000
VERSION     DANKAY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(245..466)
                     /locus_tag="PWY98_002865"
     CDS             complement(245..466)
                     /locus_tag="PWY98_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038550.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="PWY98_002866"
     CDS             complement(564..809)
                     /locus_tag="PWY98_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038551.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="PWY98_002867"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="PWY98_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="PWY98_002868"
     CDS             complement(2033..3034)
                     /locus_tag="PWY98_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL2038552.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="PWY98_002869"
     CDS             complement(3193..3459)
                     /locus_tag="PWY98_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038553.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="PWY98_002870"
     CDS             complement(3449..3805)
                     /locus_tag="PWY98_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2038554.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1223 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga atta
//
LOCUS       DANKAY010000176         3844 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000176 DANKAY010000000
VERSION     DANKAY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..888
                     /locus_tag="PWY98_002871"
     CDS             <1..888
                     /locus_tag="PWY98_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2038555.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            1000..1896
                     /locus_tag="PWY98_002872"
     CDS             1000..1896
                     /locus_tag="PWY98_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2038556.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1994..2203
                     /locus_tag="PWY98_002873"
     CDS             1994..2203
                     /locus_tag="PWY98_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2038557.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2221..2493
                     /locus_tag="PWY98_002874"
     CDS             2221..2493
                     /locus_tag="PWY98_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2038558.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2495..3358
                     /locus_tag="PWY98_002875"
     CDS             2495..3358
                     /locus_tag="PWY98_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDL2038559.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1553 a          584 c          659 g         1048 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc ataaagaggg cttttatata ttaatcacaa atcacttatc acaaatcaca
      961 agtgattaat cacaaatcac ttgtgatttg tgattcttaa tgatacaata ttactataca
     1021 aaaaaagaat ggggcgtagt tatggagaag gaaaaactaa aaatacttga agaattaaga
     1081 cgtattttaa acaataaaaa tgaagcaatt attatcttga ataattactt taaaggtggt
     1141 gttggaaagt ccaaattatc gactatgttt gcttacttga cagacaaatt gaatttaaaa
     1201 gttttaatga tcgataagga cttacaggca acattgacaa aagacttagc aaaaactttt
     1261 gaggtagaat tgccacgtgt caatttttat gaaggcttga aaaatggaaa cttggcttct
     1321 tctattgttc atttgactga taatttagac ttgatccctg gcacgtttga tttgatgtta
     1381 ctgccaaaat taactcgctc atggactttt gaaaatgaaa gtagattgct tgctactctt
     1441 ttagcacctt taaaaagtga ctatgatctc attattattg atactgtacc aacgccaagc
     1501 gtttatacaa ataatgcaat cgtggcgagt gattacgtta tgatcccttt acaagcagaa
     1561 gaagaaagta caaacaacat tcaaaactat atttcctatt tgattgattt acaagaacaa
     1621 tttaaccctg gactagatat gatcggtttt gttccttatt tagttgatac ggacagcgca
     1681 acgataaaat caaacctgga agaactgtac aagcaacata aagaagataa cttggttttc
     1741 caaaatatta tcaagcgaag taataaagta agtacctggt ctaaaaatgg aattacagaa
     1801 cacaaaggct atgacaaaaa agttttatcc atgtatgaga acgtattttt tgaaatgctt
     1861 gagcgaatta ttcaattaga aaacgaaaaa gaatagaatc acaaatcaca agtgattaat
     1921 cacaaatcac ttgtgatttg tgattgttga tgataaaata agaataagaa gaaatagaaa
     1981 gaagtgagtg attgtgggaa atttaggcgc acaaaaagca aaacgaaatg atacaccaat
     2041 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttacacca
     2101 tattataaaa attgaaacag caaaaaatgg cggaaacgta aaagaagtta tggatcaagc
     2161 cttagaagaa tatatacgga aatatttacc tgataaactt taaaaaggag tgaaaatgaa
     2221 atggcagtta cgtatgaaaa aacatttgaa atagagatca ttaacgaatt atcggcaagc
     2281 gtttataatc gagtattaaa ctatgttttg aaccatgaac tagatactaa aaatactcgt
     2341 ttactagaag tgaatctttt aaatcaatta gaagtggcac aagaagttga tttatttcaa
     2401 caaccatttg aagaattaca agctattcat gagtattggc ggtcaatgaa tcaatattca
     2461 aaacaaattt tgacaaaaga gaaagtggct taacatggcg aatattactg acttcaccga
     2521 aaagcaattt gaagatcgtt tagaaaagaa tgttgaacga ctaactaaaa atagactagc
     2581 ggttgaatcg ccaaccgctt ttttacttgg tgggcaacca ggatcaggta aaactagttt
     2641 gcgatcggca atttccgaag agacacaagg aaatgttgtt atcattgata atgatacgtt
     2701 caaacaacag caccctaatt ttgatgaact agtgaaactt tatgaaaaag acgtagtaaa
     2761 atacgttacc ccttattcta atcgcatgac agaagcgatc ataagccgtt tgagagataa
     2821 agggtataat ttagtgattg aaggtacagg acgaacaaca gacgttccta ttcaaaccgc
     2881 aacaatgctt caagccaaag attatgaaac aaaaatgtat gtcatggcag tacctaaaat
     2941 caactcatat ttaggaacaa ttgaacggta tgaaactatg tatgcagatg atccaatgac
     3001 agccagggca acaccaaaac aagcgcatga tattgttgtc aaaaacttac cgaccaattt
     3061 agaaaccctt cataaaacgg gcttatttag cgatataagg ctttacaata gagaaggagt
     3121 gaaactctat tcaagcttag aaacgccttc cattagtccg aaagagacct tagaaagaga
     3181 attaaatcgt aaagtatcag ggaaagaaat tcaaccgact ttggagagaa tagagcaaaa
     3241 aatggttcaa aatcaacacc aagaaacccc tgaatttaaa gcaattcaac aaaaaatgga
     3301 aagcttgcag ccacctacac caccaatacc caaaacacct aaacttccag ggatttaaac
     3361 cttaaaaaag taaaagagta gttaccaaaa aacggtaact actctttttt tataaaaaca
     3421 tttcctcatt ttttaaaagc tgatcgagaa ataaagcaca caactgtatt ctttcttttt
     3481 tcagttggta cattttgcag tctgtaacgt gccatttact gtttgttacc ttaacatatc
     3541 cagtcgaaaa cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct
     3601 atgttgcgtt ctcgtcacag caaacgacac gaacacactg gcgaaaatga accaacaaaa
     3661 cgaaaaaaga aaggagcgta cactgtaatt tatttctagc ttttaaaaca tgaggaaaac
     3721 agcttcactt cctcaaacgc ctaacaaaaa tggttgtcaa aaaactaaaa atcaaaaaaa
     3781 gaaaagagga aaagaaaatg gaaaaaacaa aaaaattaca attagaagat tttacagaaa
     3841 atgg
//
LOCUS       DANKAY010000177         3707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000177 DANKAY010000000
VERSION     DANKAY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            85..837
                     /locus_tag="PWY98_002876"
     CDS             85..837
                     /locus_tag="PWY98_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2038560.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            834..1748
                     /gene="pfkB"
                     /locus_tag="PWY98_002877"
     CDS             834..1748
                     /gene="pfkB"
                     /locus_tag="PWY98_002877"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2038561.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1766..3697
                     /locus_tag="PWY98_002878"
     CDS             1766..3697
                     /locus_tag="PWY98_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2038562.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1195 a          662 c          843 g         1007 t
ORIGIN      
        1 ataattttta tgaacgtttt tgactgattt atattgctag tagaaaaaaa atatgctatt
       61 atttgtattg aggtgatcgt gaaaatgctt acagaggaac gcagacaaaa aatcctagac
      121 ttgcttgaac agcagaaaat cgtgaaatca caagatcttg taaacctttt gaatgcttct
      181 gaatcaacga taagacgtga tttacaagag ctggagaata gtgggtttct agaaagagtt
      241 catggcggag ctaagaaaga gcaattattg aattttgagc agaatatgag tgaaaaatca
      301 ctcaaaaacg ttcacgaaaa acagcaaatc gccaaatcgg cagctgaaac cattaatgac
      361 gaagatgtca tttatttaga cgctggctct actacgttag aaatgattcc ttttctgcaa
      421 ggaaaacaga tcacagtagt gacaaattcc gttcaacatg cggcaaattt agttgatttg
      481 gagcttgcta ccattatttt aggtggaacg ataaagctgg caactaatgc tgttttagga
      541 tctaatgcga tagaacaact aaaaaatttt cattttaata aagcatttat gggaatgaac
      601 ggtgcccatc ttgaacaagg attcacgact ccggatccag aagaggcagc tgttaaacgt
      661 ttagccatga aaaattccga agagacattt gtattgattg atcataccaa attcaaccag
      721 ttgtccttca cctctgttgc acctttggaa gcagccacga tcataaccga gcgttgtcca
      781 ttagaattcg ttaatgaatt ccgtgaaaag acaacaatta aggaggcaat acaatgattt
      841 acacagtaac acttaatcca tcaattgatt atatcgtacg cgtagatggg ttaaaactag
      901 gcgatttgaa ccgaatgaag gaagacttca agttacctgg aggaaaaggg atcaatgttt
      961 cccgtatttt gaaacggatc gattctcaat ctacagcact agggttttta ggagggttca
     1021 ctggcgaatt tatttcgaag tggcttgaaa aagaacaaat ccagtctttg ttcacaaccg
     1081 taaaagaaga tacaagaatc aatatcaaac taaaatcaga cactgaaact gagatcaatg
     1141 gcttaggccc atctatttca gatgaagaaa tcgatgagtt gaagcaagca ttaggaacag
     1201 taggacaagg agatatcgtc gtattgtcag gaagcgcgcc agctagcctg cgaaaaggat
     1261 tttatcaaga actgattgat atcattcgag aaaaaggtgc agatttcgtg attgatacaa
     1321 caggtgatga cctaaaaaat gcacttgtta agaaaccgtt gttgatcaaa ccaaacaatc
     1381 acgaattagc tgagctttac aacacaacct tccaatctgt agaagaaatc attccgtatg
     1441 ggaaaaaatt actagctgaa ggggcacaga atgtgctgat ctcaatggct ggagacggtg
     1501 ctttgctctt cacggaacaa ggaacttata aaacgaatgt attagttcgc ccattgaaaa
     1561 attcagtagg tgcaggggat tcaatgattg ctggctttat tggggcttac agccgtacga
     1621 aagatccaat cgaagccttt aaatggggag ttgcttgcgg aagcgcaaca gctttttctg
     1681 atgacttggc agctcgcgaa ttgattgatg agttgctgcc agaagttgaa atcacaaaaa
     1741 ttgattagtt tcaaggagga taatcatgaa tatcaaggat ttaatggtca aagaagcgat
     1801 gatcatggat ttgcaagcga ctgataaaaa aggcgcaatc gatgaaatgg tccaaaaaat
     1861 gtttgatgcc ggccggattt ccgatatcga aacatataaa gaaggaattc tagcccgcga
     1921 agcacaaact tctacaggtc ttggtgatgg aatcgccatg cctcacgcga aaaacagtgc
     1981 agttaaagaa gcaacagttt tattcgcaaa aagccaaaaa ggagtagatt atgaagcctt
     2041 agacggacag ccaacttatc tgtttttcat gatcgcagct ccagaaggcg caaacgacac
     2101 acatttgcaa gcattagcag cactatctcg attgctgatt gatgcagatt tcgtaggaaa
     2161 attaaaagaa gcaaatacac cagaagaagt ccaagacttg ttccaaacag ctgaagagca
     2221 aaaagaagca gaagaaaaag cagaacaaga aacacaagct gcagaagctt ctgctcatcc
     2281 agaaagaaaa ttcgttgttg ccgttacagc ttgtccaaca gggatcgctc acacttacat
     2341 ggcagaagat gctttgaaga aaaaagcgaa agaaatgggt gtggagatca aagtcgaaac
     2401 gaacggttca gaaggaatca aaaaccgatt gactgctgaa gacatcgccc gtgcagaagg
     2461 tgtaatcgtt gcagcagaca aaaaagtaga gatgaatcgc tttgacggaa aagaactgat
     2521 caatcgtcct gtcagtgatg gtatccgtaa accagaagag ttgatcaatc tagcccttag
     2581 tggtactgca ccagtattcc atggtgatgg aaaagaaagc agttcagaag aaggaaatgc
     2641 ggatggtact atcggacaaa gaatttacaa agatttgatg aacggcgtat cacacatgtt
     2701 acctttcgtt attggtggag gaatcgctat tgcgctttct ttcatgatcg accaatttat
     2761 tggcgtgcca catgatcaat tatcaaatct agggaattac aatcaagctg ctagctggtt
     2821 caaccaaatc ggacaagctg catttggttt catgctgccg gtattagcag gatttattgc
     2881 ttcaagtatc ggtgaccgcc ctggtttgat cgttggattt gctgctggtg cattagctaa
     2941 tacaggcggt gcaggattct taggtgcact gattggtgga ttcttagctg gttatgtcat
     3001 cgtattttta cgtaaattat tcaagaactt accaaaatca ttggatggaa ttaaaacgat
     3061 cctcttttat ccagtgtttg gcttactgat tactggtttc ttgatgttgc tggtcaatgt
     3121 ccctatgaaa gcaatcaatg atgcattgaa cggtttcttg acaagtatga gtggttcgaa
     3181 tgctgcttta cttggtgcat tattagctgg aatgatggcg gcagatttag gcggtccaat
     3241 caacaaagcc gcttatgtgt ttggtacagc aactttagct acgactgttg caactggcgg
     3301 aagtgtcgtg atggcttctg ttatggctgg aggaatggta cctccattgg caattttcgt
     3361 tgctacacgt ttgtttaaga ataaattcag caaaacagat caagatgctg gtttgacgaa
     3421 catcgtgatg ggactatcgt ttgtcacgga aggatcgatt ccatttgcag cagctgaccc
     3481 gattcgtgcc attccaagtt ttattattgg ttctgcttta actggcggat tagtcggagc
     3541 tttcggaatc aaattactgg ctcctcacgg cgggattttc gttgtattct tactgagtca
     3601 cccggtcatg tatttggtgt ttattgctat cggagcagtc gtatctggtg tgatttatgg
     3661 tttattgaga aaagctcctg cagtagcaga agcatagaaa taaaaaa
//
LOCUS       DANKAY010000178         3664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000178 DANKAY010000000
VERSION     DANKAY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..277
                     /locus_tag="PWY98_002879"
     CDS             <1..277
                     /locus_tag="PWY98_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HDL2038563.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="PWY98_002880"
     CDS             420..641
                     /locus_tag="PWY98_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038564.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="PWY98_002881"
     CDS             657..1499
                     /locus_tag="PWY98_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038565.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="PWY98_002882"
     CDS             1562..3364
                     /locus_tag="PWY98_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDL2038566.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="PWY98_002883"
     CDS             complement(3423..>3664)
                     /locus_tag="PWY98_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2038567.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DANKAY010000179         3602 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000179 DANKAY010000000
VERSION     DANKAY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..186
                     /locus_tag="PWY98_002884"
     CDS             <1..186
                     /locus_tag="PWY98_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2038568.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="PWY98_002885"
     CDS             329..553
                     /locus_tag="PWY98_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038569.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="PWY98_002886"
     CDS             568..1437
                     /locus_tag="PWY98_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2038570.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="PWY98_002887"
     CDS             1418..2152
                     /locus_tag="PWY98_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2038571.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="PWY98_002888"
     CDS             2185..3093
                     /locus_tag="PWY98_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2038572.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="PWY98_002889"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="PWY98_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DANKAY010000180         3600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000180 DANKAY010000000
VERSION     DANKAY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..300)
                     /locus_tag="PWY98_002890"
     CDS             complement(<1..300)
                     /locus_tag="PWY98_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2038573.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNT"
     gene            complement(416..982)
                     /locus_tag="PWY98_002891"
     CDS             complement(416..982)
                     /locus_tag="PWY98_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HDL2038574.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1118..1771)
                     /locus_tag="PWY98_002892"
     CDS             complement(1118..1771)
                     /locus_tag="PWY98_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038575.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1821..2249)
                     /locus_tag="PWY98_002893"
     CDS             complement(1821..2249)
                     /locus_tag="PWY98_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2038576.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2254..2628)
                     /locus_tag="PWY98_002894"
     CDS             complement(2254..2628)
                     /locus_tag="PWY98_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038577.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2932..3117
                     /locus_tag="PWY98_002895"
     CDS             2932..3117
                     /locus_tag="PWY98_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038578.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3130..3378
                     /locus_tag="PWY98_002896"
     CDS             3130..3378
                     /locus_tag="PWY98_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038579.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3409..3579
                     /locus_tag="PWY98_002897"
     CDS             3409..3579
                     /locus_tag="PWY98_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038580.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT         1043 a          644 c          628 g         1285 t
ORIGIN      
        1 agtgtttaat atattattcg taggataata tgtagattcc ttaacttgtc ttttgtatat
       61 aactaaccat tcttcaacaa gctcatgaaa tgttagatca ggcttatgaa gtgttttttc
      121 gtttaatttt atatcaattt ttttatttaa ctctagcaat gccactttct gagtttctcg
      181 agatttattt ttataagtaa tgctcacttt ttttcttttt ctagtttttg gatcaatata
      241 tcgctcatta tatttatata ctttttttcc gtttttatca gttttagttt cgatccacat
      301 tgttttcatc tcctatttgt tgctataata ggaatagata agtaagccga ttatagcagg
      361 tttatttttc atcacgtcca caaactttgg cgagggaggg gcgtgttttt gttagttatt
      421 gtggtaggtt atctaaagcg tattgtgctt gctcatttgt aaaaccttca aatataagtt
      481 gatcgtacaa ttgcgaatct gacattgatg cccaatcatc gtaactttcc gctttagcca
      541 aagcttgttc gttgtaatcg acatcaacat tagccagcgc ataatcgatt gcatcacttg
      601 gatattcttc aaactctagt tgttcacgca aaccttgttc agacatacca gcccaaccta
      661 gatagctttc agctttgctc aatgcatttc tgtattcacg aggaacactt tcttcttgag
      721 taacttcttc tatagtagtt tcagtagttt cagtttcttc ttcaacgact gtagattctg
      781 ctaccgattc tgcagaagac acttctgtca cgctttcttt agttgtttca gcgacggtat
      841 ttttagcatc atcactgcct ccttgcgaac ctatagcaat aaacaccaca acaaccgcta
      901 acaaccaaaa ccatacccgt ttataaaacg gtttcttaac tttatacatt ttcccatctt
      961 gacccataac tttttttgcc atttaaatat ccctcgcttc ttgttataat atatttgtga
     1021 tctcagaaat gaggtatgag tccgtgttgc agcacgggct tttttcttta taactttttt
     1081 agagattata ggcaaaatag tagggcataa aaatgtatta ttgaattccg tatttagaaa
     1141 atcctaattg aacttcaccg gaagtctttt gctgtgttgt acgcaatgct tcttcttcag
     1201 acattccatt ctgtactttc catgcaacag gcgacatccc gtatttgtta acaaaatcag
     1261 taagtgataa agtgtcagcg tcttgctgag cgcttgtttg ttggtcttct ggattctgtt
     1321 gagatgctgc ttgttgttct ttctgatctt gactgataat attgccagca tcatctgtag
     1381 tcaatccatt ttcataaagg gccacgccga aagcttccca ctctttgttg gaccaatttg
     1441 cacgatcagc tggagttgac tgtaaagtgc gttgtttcat ctgttcatat gtttcttctt
     1501 gaggtgcggt ttggattgta ctctgactgg agcttataac tgttgggcta ggttccgccg
     1561 tagcttggtt ggagcttgta actgttgaac tagaatctgt tttagatgta gatttgctag
     1621 tagatgaact ggtttcagtt gtttcttttg ttttacttat ttttgtttct tggttagaag
     1681 tggtatctgt tgattcagct tttttattat ttgaacaagc tgaaagtagc agagcagtac
     1741 ttaacaacaa cataacgcta acttttttca ttttataatt cctctttctc gttgtaatat
     1801 gtgtgctaac acgggctttt ttatataaga aaacgataag cgctttctgg aagcccgtaa
     1861 agattcttta attcctcgat ttttttagga tattgatcat tatcttcttt ataaagagaa
     1921 acaatgagat tagcagcaaa gcaattagct tcgctttcag atttgcttct agatgttctt
     1981 gttgatacat aatagctgga taagccacga tgaaaaatag cgtgacctaa ttcgtgagcg
     2041 caaatgtaga atctttcctc agagtctcgc agttcatcat ttaaaaagat tatcgcacga
     2101 tctctaattt cttgaaactg ccccttggga ttctcgataa aaggaacgta ttgaatttta
     2161 atgcccatct tttcacaaat ataaaaagga ttagcggact ggtatttccg cttcaactcc
     2221 tcgactaaat taatcgtatc catctccata agctcacatc tttttgcctt tttctttgtc
     2281 ttctttcaca atatcccaga aagtcgctat caggatatct tttacgcgct gtatttgttc
     2341 gggtgtcaat gtttccccac cataagacat attaacattt gagtctagta gtttatcaag
     2401 ttcaaccact tcctcttttg tagcccactt gggaacatta ttatttccca ataaataatc
     2461 agttgtgaca ccaaaataat cagcaacttt ctttaagttc tcagattttg gcgaggcttt
     2521 atcccatctt cttatttgtc cattagagat gcccacctgt ctttctactt ctgctatagt
     2581 cacatgcttt tcgtctgcta attctttaat cttagtaact aaactcatta ttatcaacct
     2641 ttcaaagctg aaaagaaaat aaatagctta aaagttatat ttttggttga caattagctt
     2701 ttaagctagt atatttaatt cgtaagctaa attgttagct aaataagagc aacaaaaaac
     2761 tctactaatt taaaacattc tctcggtcgc caaacttaga aatgttattt tagaggcttt
     2821 ttataagtct tatttaacta tgtattcata atagcttaaa agctaatagg tgtcaacgat
     2881 ttagctaatt ttttagctta caaattattt gtttagaaag gagctatttt tatgtctgag
     2941 aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt caaagaacta
     3001 gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg caatcgtgat
     3061 ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat ccgatagggg
     3121 gggtaggcga tgacaaaact aaaaaaacaa gattttgtaa aaaaatataa ttattctcca
     3181 tctacttatc aacgtcgaat gtcggaacta aaaaatacag caattttctc agcggcgtat
     3241 gaacgggtca caggacaaga agtttggatc aatacagaat tatacgataa atttttgtct
     3301 ttcaaatcct ataacaggtt acgcacaaga aaggtaacac ctaaagaatt tatcgagaag
     3361 catttagttg atttataaaa aacagaacat tttgagaggt gaaggttaat gggtaaattc
     3421 aacagagcat tagtattcag cgcaccgcta atcgtctatg ctttagggct ttggggaagt
     3481 agacaagcgt tgataggaac gattgtgtac atggtttgga tttttatagg gctggatgaa
     3541 gctgagtaca aaacaaaaaa gccagtcggg agggactgac taatgagtaa ggacgatatt
//
LOCUS       DANKAY010000181         3575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000181 DANKAY010000000
VERSION     DANKAY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            23..1483
                     /locus_tag="PWY98_002898"
     CDS             23..1483
                     /locus_tag="PWY98_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038581.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1704..3467
                     /locus_tag="PWY98_002899"
     CDS             1704..3467
                     /locus_tag="PWY98_002899"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2038582.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
BASE COUNT         1250 a          555 c          718 g         1052 t
ORIGIN      
        1 aaactggagg cgtgtatcag gaatgctgga aaattacatc gagcgaaata tatttagaaa
       61 agtgtatctg tgtgaacaat tatttgaatt ccaagaaata gacatcgaac aaacagctat
      121 ttctctaagg gtgactacac caacgatcct tcacgatcta gaaagtctag ccgagtgtct
      181 agaatattgt ataaaagaac aagtaagaga gaagcataag tataaactcg tattcaaaca
      241 tggtattgct ctttctgagc tcacacagtt tctgtacggt caatcttatt ttttaaagtt
      301 tttaagttat tattttaatg gtatattcac gagtacagaa ctggctgatc ttgaatttat
      361 ttctttgagc aaagtatata caatcaaaaa aatcgtattg gattttttta aagaaaatga
      421 ttatttgaaa aataaagaaa tcattatccc agaatttgac tcgcggaaca tcttgctagc
      481 tttagtacgt tatgtcaatt gggaaggcta tgaaaataaa aatccgtatg tagaagaagc
      541 ttgtaacgag ttgattgatt atgtagagac tcattttttt aaaagaagat attcggaaga
      601 tgaaaagtcc tttatcatac gaggaataga gatcgcaatc ggaagaaagg catttcccct
      661 tggactgaaa gaagaagata agaaaagcgc agaaacgaag ccgttatttc aattagtctg
      721 tcaaggattg gaagagcaaa aaggaattct agacttacaa gaagaagatg tctattatat
      781 tttttctttg ttcaacagta gaaattatac caatgagaac atggagctgt taagaaaaga
      841 tttcgaagtt gtttataaaa attttgttct caatgaccta tcactgaatg aattgatttt
      901 acagattatt agtatcgctg aaatcgacga tgcagaaaat tttattttca agcaatcctt
      961 tttacaattt gtccgtacgt tgtgggcaga tggacaagta tttttaccgg aaaggatcta
     1021 tcttctatca gaaaaagaac aacttctgta cggaaaaatt gtagaaatat tggaagagtg
     1081 gaaaaagaaa aatcaaatcc aactaagatg gaatgaaaat ttcattcgaa aattcgtcaa
     1141 aagtctttct ttgatcaaca gtgaacaagc ggggagacag gaaatagaaa tttttgttgt
     1201 ttcagaaagt tcagtcaaac aattttttta tcgaacccaa tttcatttgt atgcggaaga
     1261 aggcatcgct gaaataaatc ctattttatt tcgttcatta gaagaattgc ctgatgaatg
     1321 tctatgtgcg aaaaaaagag tggttttgtg cgatgctgct agctatcaaa atggattaga
     1381 aactgaaaag acaaagatct atccaatctc attaaaaggt gcaaacatcc atttgtatca
     1441 tattgctcag gaacttcacc tattaaaaaa acatatttct tgatgagacc ttaattagga
     1501 gaccttctac aaagcttttt aattgtctgt agaaggtttt tatttgagtc ctgattcgtg
     1561 cgaagatcta cttatataac aagctttctc tgaaattttt ttaaaatgct attgacttag
     1621 agtcaactct aagtagtaga atagataaag tgtaacaaat ctattttcat taaaagaaga
     1681 tagtacggag gagagaatgt attatgagta cagtattaca tgtattggat gcaatcaata
     1741 gccccgcaga tgtcaaaaaa ttatctgttg aggaaatgaa acagttggca gaagagatgc
     1801 gccaaaaaat cttgcaaaag gacagcgatg taggtgggca cgttggtccg aatctaggtg
     1861 tagtagaatt aacgattgcg tttcattatg tatttgattc accaaaggat aaaatcgttt
     1921 gggatgtttc ccatcagtct tatggtcata agcttttaac tggtcgaaaa aatggttttg
     1981 aaaaaggaca ttaccgtgaa gtgagcggat attccaacca acatgaaagt aaacatgact
     2041 attttacagt ggggcacaca tctacatcta tcagcttagc aacaggttta gctaaagcga
     2101 gagatctgaa gaaagaatca ggaaatgtgg tagcatttat cggtgacggc tccctaagtg
     2161 gtggcttagc ttttgaagga ttgaacaatg gtggtgtttt acaatccaat ctaatcgtat
     2221 tagtaaacga taatgagatg tctattgatg agaatcatgg cagtttatat aaaaacttag
     2281 ctgagcttcg tcaaaccaac ggtaaaagca gcaataacct atttaaagca atgggatttg
     2341 attatcgtta cgtggataat ggaaacgatc tggaaacggt gatccgttta tttgaagaag
     2401 taaaagatat tgatcatccg attgtacttc acgtccatac ggaaaaaggt cgcggttatc
     2461 agccggcaat cgataataaa atgaaatatc attggcatgt accatttgat ctgtccaccg
     2521 gggaggcaaa gcaagtaact tctggagaaa catttaatat gatcatcatg gattatttgg
     2581 ataagaaaat gcaaacaagt acagctccta ttgccgcagt caatgcagcg ataccaggcg
     2641 cctttggact gaaaacattc caagaaaaac atccagatca ttattttgac gtggggatcg
     2701 ctgaacagca ctctatcact ttcgcttctg ctttggcttc tcagggagta cgtccagtcg
     2761 tcttccaatc aagtactttc ttgcaacggg catatgatca attatcacat gatttagcga
     2821 tcaacgagaa tccagtgatt attttagtcg gtggcggaac gatctcagct agtgaccgaa
     2881 cacaccaagg aacatttgac attccttact tatcaagtat cccgaacatc aaatatcttg
     2941 ctccagcaac aaaagaggat atgatctcta tgctagattg ggcgttcgaa caaacagcgc
     3001 agcctgtagc aatccgaatt cctacacatg gtgtagaaca tggtacagct atcgcaaatg
     3061 attatagtat tccagcctat caaacagtaa ataatggagg aaaagtagca attttagctt
     3121 taggcggttt tcttgaatta ggaaaagaaa cagtaaaagc ttttagaaaa aaaggaatcg
     3181 aagcaacgtt gatcaatcca ggaacgatca cagaaaatga cgtagaaaca ttagataaat
     3241 taaaagaaac acacgaatta gtcatcacat tagaagatgg tagtttgtca ggaggatttg
     3301 gcgaaaatat cagtcgttat tatggtgatt cttcaatgaa cgtattaaat tttggtgcag
     3361 agaaagaatt cacggatagc gttcctgtag ggcagcttta tgaacgttat catttaacac
     3421 caaaacaaat cgttgaagat agtctgcgtg tcttgaatat tcaataattc attatgctca
     3481 ccatccagat aattgtttat actagtatct ggatggtttt ttcattataa aaagcaaagg
     3541 ggtggatgtt atgtatcagc aagtagaaga atatg
//
LOCUS       DANKAY010000182         3523 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000182 DANKAY010000000
VERSION     DANKAY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            109..1875
                     /locus_tag="PWY98_002900"
     CDS             109..1875
                     /locus_tag="PWY98_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2038583.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1890..2327
                     /locus_tag="PWY98_002901"
     CDS             1890..2327
                     /locus_tag="PWY98_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2038584.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2340..3491
                     /locus_tag="PWY98_002902"
     CDS             2340..3491
                     /locus_tag="PWY98_002902"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2038585.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1183 a          631 c          767 g          942 t
ORIGIN      
        1 ctgacatact caaacctttg gcacaaagtt tcgtgccaaa gtacgactat ttctcttttt
       61 agtaagcgga tacaattaga gtatcaaaag gagagaggga gagacaaaat ggaacggacg
      121 attcccaaaa acaacatttc gctaaaagcg agggtacaaa aattaggcag tactttatca
      181 agcatggtca tgccaaatat tggagcactg attgcctggg gtgtattaac ggctctattt
      241 atcccagatg gttatttacc aaatgaggct ttggctacca tggtaagccc aatgctaact
      301 tatctgattc ctttattgat cggttatact ggcggaaaag taattgctgg tgaccgcgga
      361 tcagttgttg gggcaattgc aacgatgggt gtgattgtcg gaacagatat tccaatgatg
      421 ctaggcgcga tgatcatggg acctcttggt ggatatgcca tcaagaaatt tgatcagcta
      481 ttccaaaaac ggatcaaatc cggttttgaa atgctagtca ataatttttc agcaggtctt
      541 atcggttttg gattagcgtt attaggtttt tctgcgattg gtccagttgt cgatgcattg
      601 actcaagcaa tggcaaaagg tgtagagatc attttatctg cccatttgat tccattgaca
      661 agtattttta ttgaaccagc aaaaatctta tttttgaata atgcaatcaa tcatgggatt
      721 ttgacaccat tgggaactga acaagtagca gaggctggaa aatctattct attcttgctt
      781 gaagcaaatc ctggtccagg acttggcgta ttacttgctt ttactttgtt tggcaaagga
      841 gcagcaaaat cttcagcacc aggagccatg atcattcact ttttaggcgg gatccatgaa
      901 atctattttc cttacgttat gatgaaaccg ttattattct tatcagttat aggcggagga
      961 atgtcaggaa gtttcatttt ccaattgatg gatgctggac taagagcgcc agcttctcca
     1021 gggtcgatca ttgcgattct agcgatgaca ccgttcaatt cttaccttcc tgttattttg
     1081 ggcgtgattg ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt gaaagcggat
     1141 gcgaaagata caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc agctaagcaa
     1201 gcttccaaag gtgcccaagg tattcctcaa ggagctacat tctctggcgt acaaaaaatc
     1261 atttttgctt gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat cttgagaaaa
     1321 aaagtacagg aagcagactt gcctcaaacg gtgaccaatc aagcaatcag ccaactgaca
     1381 gatgactcga atacattgat cgttactcag gtcgaactac aagaaagagc aaaacaaaaa
     1441 gcaccgagcg cgcgttttgt ctcagtagaa aactttttga attcacctcg atatgatgaa
     1501 atcattcaag aattaagcag caagcagaaa acatcagttt ctgaaattga gaatgagcca
     1561 gcagaagagt ttctttcagc aaacatcaaa caaatcaaag agattctact tgtccacgat
     1621 gatcgttccg gatcagcaac aatggggatg accgtgctca aagatatatt agacaagaac
     1681 catttaacta tcccgttaag aaaagtcaat atccaagatt taaaagatga acctgattca
     1741 ctgatcgtag ccaaagaaag tttagcggaa aaagtacaga tggctgcacc tcaatccatt
     1801 catttatcag tcaacagcat ggtcaatccg caaaaatacg aatcgattgc acaatatttg
     1861 aaacagacag cttaagggag gaaaacagca tgcaaagttt gccattcgac atgatcgagc
     1921 tcaatcgatc ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag atgcttgtag
     1981 aagcaggggg cgttgatgag gaatatatcg atgccatggt agatagagac aaaatgttat
     2041 ctgtttatat ggggaatttt atcgcgattc ctcatggaac agatgaagca aaaaaatatg
     2101 tgaaaaaatc cggcatctgt gtcgttcaag taccagatgg tgtagatttt ggtaccgaag
     2161 aagaagaaaa gattgcgact gttttgttcg gtattgcagg agtcggagat gagcatttgc
     2221 aacttgtaca gcaaatcgca ctttattgca gtgatatgga caatgttgtc cagctagcag
     2281 acgcgctcag caaagaagaa gtaacggaaa atctagcaat tgcttaagga gagagaaaaa
     2341 tgagagcagt acattttggt gcaggaaata tcggtcgagg atttattgga gaggttttgg
     2401 cagctaatgg atttgagatt actttcgtag atgtgaatca cacgatcatt gatgctttaa
     2461 atgaaagaaa agaatacgaa atcgaattag cagatgaatc aaaagaaaaa atcactgtaa
     2521 aaaatgtcaa aggaatcaac aatcaaacaa atccagaaaa agtaatccaa gaaatcgctg
     2581 aagcagattt agttactacc gccattggcc cgaatatctt accttttatt gctgagttga
     2641 ttgcaaaagg tatccaagaa agagaaaagg aaggaagcac aacatcttta gatatcattg
     2701 cctgtgaaaa tatgattggc ggttcagcat ttctagaaaa agaagtgtat aaatatcttc
     2761 cggaaacatc atttactgac aaatatatcg gctttccaaa tgcagcagtc gatcgaatcg
     2821 tcccattaca acaccatgaa gatcctttat tcgtccaagt ggaacctttc aaagaatggg
     2881 tcattgacga tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt ttatatgtag
     2941 atgatctaga accatatatc gaacgcaaac tatttagtgt caatactggt catgcgactg
     3001 tcgcttatac aggtgccttg ttaggttacc agaccattga tgaagcaatg caagatgcgt
     3061 tagttgtggc acaattaaaa tcagttttgc aggaaacagg aagtttgctg attgccaaat
     3121 gggggtttga tgcagagcag catcatgcgt atattgaaaa aatcattcat cgcttccaaa
     3181 acaaaaatat ctctgatgcg atcacacgtg tcgctcggac accattacgt aagttaggtt
     3241 atcaagaaag atttacacgt ccagtgagag aactacaaga acataacttg acttgcccac
     3301 atttgacggc aacaatgggg attattttta attactatga tcctgaagat gaacaaagcc
     3361 gtcaattgca tgaaatgaag atccatgaaa acttggaaca attgattcaa gaagtcacag
     3421 gaatcaatga tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt tatgccaagc
     3481 aagtcgctta aaataggata cgaaaaaggg gaatgtgacg aaa
//
LOCUS       DANKAY010000183         3507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000183 DANKAY010000000
VERSION     DANKAY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            248..1468
                     /locus_tag="PWY98_002903"
     CDS             248..1468
                     /locus_tag="PWY98_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2038586.1"
                     /translation="MKKPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFP
                     LNIINTSITMIRKANSDLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVI
                     FRYRLTDLFYGEIEKKYPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQ
                     KEHFSSKKVAIVHTTGKGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFT
                     TIPLKNVNEETPVIHLTNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQE
                     YLNKLVAPLIRGYYVDEEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLI
                     TDQGLAETGGNGVIFLFAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQES
                     FLKLIKSKGERLHE"
     gene            1461..1952
                     /locus_tag="PWY98_002904"
     CDS             1461..1952
                     /locus_tag="PWY98_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2038587.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            1940..2494
                     /gene="srlA"
                     /locus_tag="PWY98_002905"
     CDS             1940..2494
                     /gene="srlA"
                     /locus_tag="PWY98_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2038588.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            2512..3507
                     /locus_tag="PWY98_002906"
     CDS             2512..3507
                     /locus_tag="PWY98_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2038589.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1200 a          572 c          717 g         1018 t
ORIGIN      
        1 ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag gagatgagct
       61 agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga cgaaaacaga
      121 acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga atgctagtaa
      181 acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa tctctagtaa
      241 ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata tccaagaatt
      301 caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc aatatgaaaa
      361 agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga taaggaaagc
      421 taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac gattagatct
      481 cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct ctttattgat
      541 aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga tagaaaaaaa
      601 gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag ctatcttgca
      661 acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt tagcatttcg
      721 atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga caggaaaagg
      781 aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata tacaaataac
      841 acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg ctgtttttac
      901 aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga ctaatctttt
      961 tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt
     1021 tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc aagaatattt
     1081 aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat ttgaaaaaag
     1141 gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg cttttcctca
     1201 cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa ctgatcaagg
     1261 tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg aagaaatgac
     1321 ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac tcataaaaat
     1381 gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa aactaatcaa
     1441 aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc gcagcttcag
     1501 cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat caaacatata
     1561 gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa atccgttcag
     1621 gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc agaaaaatgc
     1681 aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt gaagatatcc
     1741 attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca caagcagctt
     1801 tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac
     1861 cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt acgatcaaag
     1921 aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat ttatgaggct
     1981 gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac cagttgtctt
     2041 gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc gagtaacgaa
     2101 agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac cgttcttatc
     2161 tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc cagaatacta
     2221 caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg gtgtattccc
     2281 ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga tcgaaacctt
     2341 agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc tgatgaactt
     2401 tattggtggt tggataactg attttactac tggatttgta tgcagacagc aaggaatcgt
     2461 tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta aatgtatggt
     2521 gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat taggccgaca
     2581 aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat gatcgtagat
     2641 aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc aatacctgat
     2701 agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg aatttatcct
     2761 aaaaaaggga taccaaccat caatatcgtt tccacaggaa aaagtggccc attagcacaa
     2821 tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca gccactaatg
     2881 gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa gtacaaatat
     2941 gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat agcaaaaatt
     3001 ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga agcagtagat
     3061 acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat tggtgtgata
     3121 caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc aggaaatgca
     3181 gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc tttgcttggt
     3241 ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat tgggaaagga
     3301 aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca aaatgcatgt
     3361 gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt agaagtaggt
     3421 gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat cattgcttgg
     3481 gtagcaagtt ttggattata tagctga
//
LOCUS       DANKAY010000184         3483 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000184 DANKAY010000000
VERSION     DANKAY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(110..787)
                     /gene="nth"
                     /locus_tag="PWY98_002907"
     CDS             complement(110..787)
                     /gene="nth"
                     /locus_tag="PWY98_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2038590.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(794..1492)
                     /locus_tag="PWY98_002908"
     CDS             complement(794..1492)
                     /locus_tag="PWY98_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2038591.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1760..2944
                     /locus_tag="PWY98_002909"
     CDS             1760..2944
                     /locus_tag="PWY98_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2038592.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2950..3393)
                     /locus_tag="PWY98_002910"
     CDS             complement(2950..3393)
                     /locus_tag="PWY98_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2038593.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          887 a          703 c          590 g         1303 t
ORIGIN      
        1 ctactgagta tgtacattta ctttttcatg caaaaaagat aaacgaaaca gcttttctcc
       61 ataataacaa gataattcat acatttattt gcaattaaag aaaccacgtc tacaggcgtg
      121 gtttcttttt tagcgccttt tcttttagac gcatccgatt tttcccatct tgacatattg
      181 atagcaaagg acatacctcg cacttgggat ttcttgccgt gcaatgatat ctaccaaaaa
      241 aaatcagtgt atgatgtgtc tttacccata attcttgtgg tactttccgc atcagtgttt
      301 cttctacttc catgaccgta gcatctagtt tgcagatcct caggcgtttt gagacacgtt
      361 ccacgtgggt atctactgcg attgctggta tcccgaatgc atcccccaat acgacatttg
      421 ctgtctttct gcctacacca ggaagagaca tcaattcttc tcttgacgtt ggcacttgcc
      481 catcgaaacg ttcaatcagc tgctgtgcac acgctttgat gttttttgct ttatttcgat
      541 acaaaccaat cgtttttatt ttaagaatga tttcatcgat tgaagcttct gctaacgcat
      601 cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt attcactgat acatctgtcg
      661 cctgggcact taagatcact gcaatcaata actcaaatgg attattgtgc ttgagttctc
      721 catgtgcttc gggaaacatt ccatacatcg tttctagagc ttccattgtt ttttgtttgc
      781 ttaacatatt ccgctactcc tccaaccaat ttttcagtga gactttcggc aattcttctt
      841 gttgagctga ctgttctact tctctttgaa gaagctgctg ttttcttcgt ttttggtctt
      901 cttccacttg ttgttttgta cgtagatttt ttctttccca cgatagcaaa atgcggtcaa
      961 cgtaattaaa actataagcc tgatttaata cggcttcccg cagtgctagc ttcaaaattt
     1021 ccggctgata atggtcttct tctagccatt gtccgatccg ctggaactcg atcgaagaaa
     1081 gcgggcgtcc aaattcttgt tcaaataatt gatacactga ctggatttct ttttcttgtg
     1141 ttttttcttc gtattttttt tcttgtgttt ttaaatactc acctaataga tcatagatgg
     1201 ggtaaagatt atatcgatct gcttttttac cgttgacaat cgtcgtttct atcttgataa
     1261 atccatttgt tacgagacga tccagtattt gaaaaatctg atcttgtttg attcccatat
     1321 ccatagcaat catctgcaaa tcagggaaag tatctccttc aagctgtgcc atatgcaact
     1381 gtagaacaaa taaaaattct tcagattgta gaccgatcct atgataattt ttaagtaaaa
     1441 gattagatat gactgtttgt ccagcgctta agtaattttc taaattcaac atgatttctc
     1501 ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag caagaacagg tttcttttct
     1561 atttacttca ctgatcggtc agatttacaa tgtcgtctac ttgatttttg tttgttcatg
     1621 tttttgatgc gtatcttcac ttttcggaat aaataaatta agtagatttc gctgttcctt
     1681 agaatgttga caaggaaaca aaattactat acactatttt tgtttccaag ttaacattat
     1741 tgcataaagg agtttttaca tgtacagttt cacaaataaa cattctaaag tttttggata
     1801 cgtctctgtc ttattcactg gattaggatt tactatcgtc agtcctgtac ttccgttttt
     1861 aacgctgcct tattctcact ccatacatca gcaagctttc tatatcactt tgctcatgtc
     1921 tgtttatgct ctagctgcct tcttatctgc ccccattctc ggtagcttaa gtgatcattt
     1981 tgggcgtcat ccgatcctta ttatcagttt attaggatca agtatcggat atttgctttt
     2041 tggtttagga aattcgatat ggatgttgtt ccttggtcgt atcattgaag gattgacggc
     2101 aggagaaatc tccaccttat atgcttactt tgccgacatc acggaaccta atgaaagaac
     2161 caaagttttt ggatggatgg gtgctttagt cggtatagga acaacgctgg gtcctattat
     2221 agggggtcta ttagctgagt tggggaattc tgttcctata tttattggag cactctttac
     2281 tttcttaaat gccgtttatg gctacacatt tatgctagaa agccttccaa taaaaaaacg
     2341 ttcagtagat ttaagtttct cccatgtaag accttttcac caattacaac aattattcaa
     2401 gatttcatcc gttattcctt tattgacagc aggttttgct gtttggttgg cagctggctc
     2461 tcttcaatct atcttttctc aattttcgat tgacgccttt cagtggaagg ctgggttggt
     2521 tggtttaagc ttctctttga ttggaatctt ggatactgtc tcgcaattgt tcattatgcc
     2581 tcgattgctc aaaaaatttt cagaacagca gatcacaaga atagggatgt tcagtgaaat
     2641 cctcgcctat ctattcatca cattatctgg aattcttcta ctgccgattt tgtttttatt
     2701 tgggattatc tgttatggtt tcggtgattc tatttttact cctgtattca atggccaact
     2761 atccaattct gtctcagata accagcaagg tcttgtaatg ggcggtaccc aaagtataca
     2821 atctctatcg cgcgtcatcg gcccgctaat cgcaggacag ctttatgctg cagcaccatt
     2881 tcttccgacg gtattcggat ttttgcttat gatttttgcc tattatcact acaataaaaa
     2941 atagtagctt catttgctac ttcgtataaa ttctctgtat cgaccgataa aacgaaatac
     3001 tgtgtcaaat tcttcttttg acatattttc aaatacagta gcatctcgct gtacccattt
     3061 ttgatggagt tgctcgtgtc tattccttat tttttgtccg attggcgtca attgaaaata
     3121 gatttctttt ttattctctt cgctttgata agtgtttatg atccctctag ctatcagttt
     3181 ttttgttagc ttactgatgg ctcctctagt catgtaacta gcattcgcta attttgtcac
     3241 gttaggatca gggattttcc cgatatgttc aatcacttct agttcattta gagtaaatcc
     3301 tgcaaattct ttttccattt ctggtttatt acgatgaata atctcattag ctaaatccag
     3361 atattcttct attagcagtt gtcttttatc catttctaca ccttcttttt ctttcattat
     3421 attgtttttt tgtttcctat tcaacaaaga taaaaatatt ttgtaggaaa acccaatctg
     3481 aaa
//
LOCUS       DANKAY010000185         3396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000185 DANKAY010000000
VERSION     DANKAY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..915)
                     /locus_tag="PWY98_002911"
     CDS             complement(<1..915)
                     /locus_tag="PWY98_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2038594.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLK"
     gene            complement(912..1565)
                     /locus_tag="PWY98_002912"
     CDS             complement(912..1565)
                     /locus_tag="PWY98_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2038595.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1853..2373
                     /locus_tag="PWY98_002913"
                     /pseudo
     CDS             1853..2373
                     /locus_tag="PWY98_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2275..2991)
                     /locus_tag="PWY98_002914"
     CDS             complement(2275..2991)
                     /locus_tag="PWY98_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2038596.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3041..3250)
                     /locus_tag="PWY98_002915"
     CDS             complement(3041..3250)
                     /locus_tag="PWY98_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038597.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
BASE COUNT         1075 a          660 c          455 g         1206 t
ORIGIN      
        1 tttcaaatat tcttcatcat tattcaaatt ttctatagaa tgaactgata atatttgaga
       61 aatatttttt gttaaactat caacgatttc aatacattca ttaatataaa tatctctttc
      121 tttatatttt ccaatattgt atttcatgtt ttccagtatt attttaagac tagcaagagg
      181 ggttttaagt tcatgggatg cacctttaaa aaagtcatat tttaactttt ctaattttaa
      241 aatttcttta tttttaaatt ccaaatcgtc aattgttctt aataaagtag aatacaaatc
      301 attaatttgt tgttttaatt ctccaacttc atcattagag ctaactttta aactcatttt
      361 tttatcaagt ttcatcattt tatcagtaac aatttttatt tcttgtatat tattttttat
      421 tgattttgca taaattaaag aaataatagc agaaaataaa attgatatta ataaagaata
      481 tggtaaaaat ttaaggctta aatctttcgc atctttttgc atatcagctg tagaaacaaa
      541 ttgtagattt gtttttttac catcattaag ctttatttct ctttcttcaa ttatcaaaga
      601 attactatca ctttctaaat taacatttat attatctttg atttgtattt cattcttatt
      661 atttttttct tttatgaata cttttatttc actactcttg gaataaaact ccaaagtttg
      721 ttctatatat tttatttctt ttccattcat attactagaa atttcatttg ccatattatg
      781 aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa ttaatgaatg
      841 aactaatata attattattc caagaacaga aaatatttgt atgaacattt ttggaaatat
      901 ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt agttatacag
      961 tctaattgta atttttttct aagttctttt atatatacat ctattactcg atcataagga
     1021 gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa cacttgtcca
     1081 ttattatcca ataaacattt taatacttct agttcttttg catttacatc tatttcttca
     1141 ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata ttcaaacttc
     1201 tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa gaccggcaat
     1261 gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat tttatattct
     1321 tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct aatttctttc
     1381 aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa atttatatcg
     1441 ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt atatccaaat
     1501 tctgaaagat attcacttat tccctctctt atcatattat catcttcaac agttaatatt
     1561 ttcatatata cctccactta agcctataca ctagatatta tacagcatat tatggctcta
     1621 taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt ttaataaatg
     1681 caaacagaca ccttgaaatc ctttagaaaa aaggcttcta taatattttg tgttgttgga
     1741 aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa agagactcac
     1801 ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct ctatggataa
     1861 tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg aagaatggct
     1921 gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat tggatgactc
     1981 accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt cctatacgac
     2041 acgtactcag atgttgaaag tcaaagaaaa gttgactatt ttagaattaa aacgtacccg
     2101 ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag ttgatgaaca
     2161 tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg aaaatcaatc
     2221 acgtaatctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa actcttactc
     2281 aaagattcca cgacattgga gccgctcaaa cggcattatc atcgactgtt taaacgacca
     2341 attagtcaaa cagaaatcgt cgatgaaccg ttgtgcacaa tggtgtcctg aacgtacagc
     2401 gacaccttcc atatccagag cagtcgccac atcacgggga tggatgccat ccaaattaaa
     2461 agcaaccacg ccagtatgat gttcaggatc ttgcggtccg taaatggtga taccgccgat
     2521 cgcttgtaat ttaggtaaaa cgtaggcaac cagttcttgc tcataacgat gaatattctc
     2581 cataccaatc tcattcaagt aatcgattgc tcctgcaatg ttcggagtac cagcttcaaa
     2641 tttccatggc agctctttcc atgaactatc gtaaagattg acaaaatcta tcatttcacc
     2701 gccgaattcg actggctcca tttgttctaa caattgacgt ttgccatata atacaccgat
     2761 tcctgtcggc ccgcacattt tatgcccgct aaaggcgaaa aaatctgcat ccaacgcttg
     2821 tacatcaact gtcatatgcg gtgctgcttg ggcaccgact ggactatcct caactacaac
     2881 agcctgttca accctcagcc gtttcaagca caacaaataa ccttctggat ctggtttgcc
     2941 gacctgaaga tcatcgatcg tcacaaagga atcaattttt tccttaatca tcggatgtgc
     3001 ttcaaaaaaa tagtctagct cccgcagttc agaattgaga ctactccgat tttccagtgc
     3061 tgctgatccg ctgctcaaaa tgttcagcat ccactggtct cccaaccgct tgacttatcc
     3121 cttcataaaa ggacagttct gctgccgtcc caacaccttg ttgatagtct gcaaaaggaa
     3181 ttttatagcc gaactgctcc agtaaatagg cttcagtaaa tagtgatgaa tactctcgta
     3241 aaacatccat tcactgtcaa tcatcgtccc atcaaaatca aaaataagtg ctttggtcat
     3301 cttctttccc ccttacccaa aacaagcaaa agctgtgtcc tgctctctct acagtatcag
     3361 cataaaaact gccgtctgaa aagtcaaagg tgaaag
//
LOCUS       DANKAY010000186         3347 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000186 DANKAY010000000
VERSION     DANKAY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..229)
                     /locus_tag="PWY98_002916"
     CDS             complement(<1..229)
                     /locus_tag="PWY98_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2038598.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIW"
     gene            387..650
                     /locus_tag="PWY98_002917"
     CDS             387..650
                     /locus_tag="PWY98_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038599.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            674..862
                     /locus_tag="PWY98_002918"
     CDS             674..862
                     /locus_tag="PWY98_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038600.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(945..1130)
                     /locus_tag="PWY98_002919"
     CDS             complement(945..1130)
                     /locus_tag="PWY98_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038601.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1345..1716
                     /locus_tag="PWY98_002920"
     CDS             1345..1716
                     /locus_tag="PWY98_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2038602.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1866..1938)
                     /locus_tag="PWY98_002921"
     tRNA            complement(1866..1938)
                     /locus_tag="PWY98_002921"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1903..1905),aa:Gln,seq:ctg)
     gene            complement(1984..2412)
                     /locus_tag="PWY98_002922"
     CDS             complement(1984..2412)
                     /locus_tag="PWY98_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038603.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2472..3230)
                     /locus_tag="PWY98_002923"
     CDS             complement(2472..3230)
                     /locus_tag="PWY98_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2038604.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1116 a          526 c          627 g         1078 t
ORIGIN      
        1 tccaaataat aagaaaaatt actgaaagcg tcaattcgat gatgtgtgtt attaatacag
       61 acgttgtttc ccaatgccta aaagaaaaaa tttgataagt aaaatataca gcaggtaaag
      121 aggccagtaa aactgaaaac ttcttttttg tccattttat gtaatagaaa agaatgctta
      181 tcataataaa aaaaggaata taaaaaacaa attgaccgat cgcgatcata ctattgcctc
      241 cgataaataa agtccatgat acaaaataac actttgtgcg aaatggcgca agcagttttg
      301 tggatttatc cggtaaaatc tgtatcgatt ttgaaattag aggagaaact tgctataatc
      361 aaagtgaaag aaggagtggg attttcatgg atcatgtagt gttagaaatc gatttgcatt
      421 tgatcaataa tattcgggtg atttactatg ttgtaagtaa cagtgtcgaa caacgtgtac
      481 tgacgaataa aataaatggt attttagcaa agaaagacgt tcaccgattc aataatggtg
      541 aaggaagtta ttatagtatc ccagtagaaa aaattattta tacgacagtc aaagtcagag
      601 aagatctaga taccaaaaaa gcatatgaac ctatttttac aacttattga ttgacagcag
      661 atggaggaaa aaaatgaatc catatgaaca acttattcaa gatttgatcg atggaaaaat
      721 cgaaaagatt gatgtaaaaa gagaagaatt aatggctttt cgagaagcgt ggctgaagtt
      781 ggagaatcgt aaatatgtgg tgggaaaagc ctcactgaac ggcaatgtga cttatcatta
      841 cgatccgaca cgtttatttt agtatgaagt attcagaaaa gaaccaaagg aggacgatgc
      901 agcagcattg tcctcctttg attttacaaa agtgaaagtt ttacttattt gaatagattt
      961 ttaaatttgt ttttgacact gtcgatattt acatctttta aaagattttg ggctttctct
     1021 ttataaggtc ctaattgttc tttatgttct tcgataaatc gttttgcgcc atcgaaattg
     1081 ccgttcttaa tcatttcctt gactttttga aataattctt cttgattcat tttgaacaca
     1141 tcctttgcta tcaacatgat tttttacatg gacaaaacga gaaaaactga tttatccaca
     1201 cgtcaataat atcaaataac gatcattttt actaaaaaaa cactcgattc tcgcttctaa
     1261 agtaggagaa aaatctccaa cttgggacgg atggaatttc tttaactgac ccatatactg
     1321 aatatataac aaggaggtag aaagatgaaa gtgattaaat atttgattgg actggcggcg
     1381 attagtggta ttttgctatt tggactgaaa tattatactg aaggaagtta tggagaaatc
     1441 cctggtatac ttgatcagct aaatccatta gttgaaaaag gagaggtcta tgtaaagaca
     1501 caaaaaccag aggaagtaaa tgaatatggt acagcaaggt atgtgcagaa agctgctgat
     1561 gccaatggga aagaacgaac ggttgaattt aatggtcttt ctgtgttaaa agaaaatcat
     1621 tatttgaaaa ttagcaataa aggagcacac atagaaacgt atgaggaagt tcctagagaa
     1681 aaagttccgg agaaagcatt agcaattatt gggtaaatca gtttttgtat taagaatacg
     1741 cccgatttta ttattcataa tttaattatg taaactaaat acaagtagta aaaataatac
     1801 aataatcccc ctgcaaaaag attgttcttt tacagggggt tcattttttc agcaagcaca
     1861 tcaaattggg atggcagggt tcgaacctac aattgcttga ttcagagtca agtgccttac
     1921 cagtttggct acatcccatt tattcaacaa aataatcata gcactttcct aaaaaaacgc
     1981 aagttatttt tcttgatcgg atagttgttg ctttactcgt aaaaggcggc gtactagttt
     2041 cttttctaat ccagcaggaa gggcaatcag ttctgctact gacaagacgg tgtccaataa
     2101 ctgatattct ttgacaggtt cattcataat atgatagatg tttgctaaac gttcaatata
     2161 ttcaaagcga gtatactctg ttcgagcaat tagaaatttc aataaatcgg tggctttctc
     2221 gtactcttga tctgcgtaat aaacaagaga ttgacgatac aatttgattt ccagttcatt
     2281 gagggtagca ggtctgcgac gctcgttcca ctcattaata gagactttac cttgaacatt
     2341 tcccttgcag tcacggacaa aaataatcgg aatttcattt ggatccattt ttttatgtgt
     2401 gaatttttcc attatctacg ctcccttcaa aggtttggta tcttgagtat aagggatttt
     2461 tgtttgaatt actagctttt ctccaaagtt agcatctgtc cacccattgc agtgaaaatc
     2521 gccgaaaaat cgttgatcgg tactgctcga ttttttacac catagggatc gttgacataa
     2581 atattttctt tgtcatatcc agtgactaca caagcatgaa caagaggagt gactcttact
     2641 tctccattat ttgtggtcca agtcataaaa tcagtttcat ctgggacttc aaaattgata
     2701 gtcacagaag tccagacagg atttccttta gatagtgctt ttttgatatc atcgagttga
     2761 gcatcggttt ttggaaaagt gtgataatcc gtttggacga tttgctgagc aagggaggca
     2821 actgggggaa catagacgac catagcccag tctccgccac tgatatcacc aacaaatcct
     2881 tcattaggat cgccatgagt cccatctgca ttgaaaacag ggacgtaatc caattgctca
     2941 gctaactggt ttttagttgt ttcataacca taaaaagata aaagcataga aagcgaagtg
     3001 acttcacagc cattttctaa agaaggagaa tcaaactggc tctctaatgg aacatctagt
     3061 aaaacttctg ctttctcttt tgtctttttc gttttttgtg ttgaactttt ttgtgcagtg
     3121 ttcattgtat gttctggaga atggatatgt aggtaataac ttgtaccgct taataaaaga
     3181 gcagctgtgc cccctagaaa caatagtttt ttgaacgttt ttttcttcat ttgaaatctc
     3241 ccttttcagc tgtttatctt tcctattata aatgtatttt tattggaaat ggaagtatta
     3301 tattttaata tgtaggaaat aattagtttt taaggaatat gttttaa
//
LOCUS       DANKAY010000187         3342 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000187 DANKAY010000000
VERSION     DANKAY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="PWY98_002924"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="PWY98_002924"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2038605.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="PWY98_002925"
     CDS             complement(1034..1954)
                     /locus_tag="PWY98_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2038606.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="PWY98_002926"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="PWY98_002926"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2038607.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          954 a          667 c          586 g         1135 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag ta
//
LOCUS       DANKAY010000188         3278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000188 DANKAY010000000
VERSION     DANKAY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..2232)
                     /locus_tag="PWY98_002927"
     CDS             complement(<1..2232)
                     /locus_tag="PWY98_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038608.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="PWY98_002928"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="PWY98_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          897 a          732 c          535 g         1114 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttcta
//
LOCUS       DANKAY010000189         3240 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000189 DANKAY010000000
VERSION     DANKAY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            36..1043
                     /locus_tag="PWY98_002929"
     CDS             36..1043
                     /locus_tag="PWY98_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2038609.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1055..2059
                     /gene="neuB"
                     /locus_tag="PWY98_002930"
     CDS             1055..2059
                     /gene="neuB"
                     /locus_tag="PWY98_002930"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2038610.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2056..3204
                     /gene="neuC"
                     /locus_tag="PWY98_002931"
     CDS             2056..3204
                     /gene="neuC"
                     /locus_tag="PWY98_002931"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2038611.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
BASE COUNT         1228 a          415 c          618 g          979 t
ORIGIN      
        1 ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag
       61 taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac
      121 caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc
      181 tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg
      241 attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag
      301 caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa
      361 tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag
      421 tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg
      481 ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca
      541 tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata
      601 ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat
      661 tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg
      721 caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg
      781 aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag
      841 agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc
      901 caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg
      961 catactcttc agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag
     1021 agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc
     1081 agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc
     1141 taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc
     1201 caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt
     1261 tgaaatgcta aaaaaattag aattatctca atctgatttt attagaataa aagattattg
     1321 tatacaaaat aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct
     1381 aaaacaaatt ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt
     1441 tcttagaaaa atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat
     1501 tcatgaggtc agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg
     1561 tacttcaaat tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa
     1621 agaaatgttc gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat
     1681 ttgtgctgtt gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact
     1741 acctggtcca gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca
     1801 tattagaaac actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact
     1861 tagtacaaaa gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga
     1921 tattcttaca gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt
     1981 tttatatcaa tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct
     2041 tgaggaagat gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga
     2101 atatataaac ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt
     2161 tctggaatgc atttatccca taaatatgga tttactaaag aattgatctt agctgacgga
     2221 tttagaatta gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct
     2281 cggtctgtgg cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat
     2341 tttgtattat tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat
     2401 cttggaatca aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca
     2461 ataagacatg ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa
     2521 gagcgtttaa taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga
     2581 atagatacta ttttaaataa tagattacct agttttagag atataaaaga aaaatatcat
     2641 ctaaaatttg aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta
     2701 gaaaatatag aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca
     2761 attatttgca ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag
     2821 aaatatttaa aaacggatat tatttatatt acgaatttag agccggagga atacctgact
     2881 atattaaaag agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca
     2941 tcattcaaaa taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa
     3001 aatattataa atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa
     3061 gatcctcagt ttataaaaag cttgaaaaat gttaagaaca tctacggtga cggcaacagt
     3121 tctagaagaa taacaaaaac attgaatcaa atattgaatg atggggaaga tattaagtgg
     3181 attcaaaaac aaatagctta ttaaaatata ctagtgatcc atacattatt attggggctg
//
LOCUS       DANKAY010000190         3216 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000190 DANKAY010000000
VERSION     DANKAY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(554..2167)
                     /locus_tag="PWY98_002932"
     CDS             complement(554..2167)
                     /locus_tag="PWY98_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2038612.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="PWY98_002933"
     CDS             complement(2308..3216)
                     /locus_tag="PWY98_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038613.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          552 c          572 g         1054 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccac
//
LOCUS       DANKAY010000191         3207 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000191 DANKAY010000000
VERSION     DANKAY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            247..2412
                     /gene="acm"
                     /locus_tag="PWY98_002934"
     CDS             247..2412
                     /gene="acm"
                     /locus_tag="PWY98_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2038614.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2684..3169)
                     /locus_tag="PWY98_002935"
     CDS             complement(2684..3169)
                     /locus_tag="PWY98_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2038615.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
BASE COUNT         1210 a          478 c          609 g          910 t
ORIGIN      
        1 atatagattg aaaaaatgaa agcaataagt aaaataaaat gaatatatgc gaaaaaataa
       61 tttacattag gaaaaacctt gtaaaaacag ttttttggca tatttatttt ttataaatat
      121 ttgtaaaata actatcaatt ttttagttaa aagattggaa atgagtgggg gaggaattga
      181 agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat aggggagata
      241 gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat tactaatata
      301 acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag cagtaatgtc
      361 acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa agtaaaattc
      421 tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat caattggcca
      481 agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat gattgaaaat
      541 aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt taatgaaaat
      601 attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc cagaaacgta
      661 accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga taaaacagct
      721 actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta ttataaaaca
      781 ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat taacaataac
      841 ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg acaaagatta
      901 gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt ttatagagga
      961 gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa tttttcagta
     1021 actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag gaaaataatg
     1081 gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa tcattcggaa
     1141 gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa tcatacagtt
     1201 aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt gaagattttc
     1261 aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt aagacgagca
     1321 gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga acagggagaa
     1381 gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga agcacccaat
     1441 tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa cgaaaatgat
     1501 acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat cacagcaact
     1561 aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt attcagagca
     1621 agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga caatggaata
     1681 acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga atatacgttt
     1741 aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta tcaaaaaatt
     1801 gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc aggacaaaaa
     1861 acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac agtaaatcta
     1921 ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga ttggcgctat
     1981 cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac tgtaacggaa
     2041 gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa cagttataca
     2101 ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa aaattttcca
     2161 gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca aatatttgct
     2221 catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc tgtacctaaa
     2281 cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa atctagtttt
     2341 gctgtaatca ttggtttatt gatagtaact atctgtgctg ggatattctt acaaaaatat
     2401 aaaaaagttt agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa
     2461 aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat
     2521 caataaatat ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata
     2581 ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa
     2641 gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa
     2701 ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg
     2761 tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga
     2821 tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg
     2881 ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt
     2941 ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat
     3001 agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg
     3061 aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac
     3121 ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc
     3181 ctcctttttt tattagtgta tcataaa
//
LOCUS       DANKAY010000192         3202 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000192 DANKAY010000000
VERSION     DANKAY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..255)
                     /locus_tag="PWY98_002936"
     CDS             complement(<1..255)
                     /locus_tag="PWY98_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2038616.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRT"
     gene            908..1003
                     /locus_tag="PWY98_002937"
     CDS             908..1003
                     /locus_tag="PWY98_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2038617.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1306..1551)
                     /locus_tag="PWY98_002938"
     CDS             complement(1306..1551)
                     /locus_tag="PWY98_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038618.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1896..2180
                     /locus_tag="PWY98_002939"
     CDS             1896..2180
                     /locus_tag="PWY98_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038619.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2183..2599
                     /locus_tag="PWY98_002940"
     CDS             2183..2599
                     /locus_tag="PWY98_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038620.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2736..>3202)
                     /locus_tag="PWY98_002941"
     CDS             complement(2736..>3202)
                     /locus_tag="PWY98_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2038621.1"
                     /translation="EDEDSIRYFVLFTGFEINKTKKFVEVSVNPDLEYIINGLTAEFS
                     RFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIPEYYQMGNIDQKVL
                     NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISLRNWLEE"
BASE COUNT         1066 a          597 c          500 g         1039 t
ORIGIN      
        1 agtacggtaa gtaagattaa gcatatcttt atagagcttt tctaaatcac cgacaaaggc
       61 ttttgtagca gtcatcttat aatcacttaa ctcttttaat tcctcgaaag aaaatctaac
      121 ttttttcaaa ccacgatctt tcatacgagc gcataatgca aaaaataaat ccatctgctt
      181 agcattgaaa tttttcagcg gtactaaatt caattcattt tgatatttga ctgttaattc
      241 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
      301 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      361 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      421 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      481 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      541 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      601 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      661 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      721 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      781 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      841 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      901 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      961 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
     1021 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
     1081 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
     1141 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
     1201 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttt cattggtcca
     1261 agctaaaaaa ctcttagaag cgattttaga gccatttaaa cccacttaat tatattctcc
     1321 atgaaattcg ctccatgcgt caaaagctgc gtctgaaatt ccgccgtctt cgttcactag
     1381 ttgcattaat ttttccaacg atcgttttct ttcgggtgtc atatttttat attccaacaa
     1441 tttttcgtct gttaaatcga acttgattgc ttgaattgct tgctctaatt tttctaagtt
     1501 ttgaaaatat tcgtaaggaa atttttccat tttttctttc tccagtctca caatttttct
     1561 agaaataaaa tcacttcttt gccgtctata ctctcaaaaa aaatgtcgtt tgaaaaacta
     1621 aataaatttt tgatcgtgca tttttaaatt tggttatcga ttacacgtgc agcactcccc
     1681 cccgaatttt aaagagcata aggaaacgtg tacccacttt ctaaaaaaag tgggtagata
     1741 ggctgtgcct atcacgtttc tcagaaaaaa atttgatatc actttttcgc catcgtgggt
     1801 agcttatgta tagcattttg gcaaaaaaca gctatacaaa tatagtattt gtatggtata
     1861 cataaattat acataattgc aaaaggagat tttttatgga cattttagtt cggaatattg
     1921 atccaaagtt tgttgcaaca atagacaaac gttgccaaga actcacgaat aaaacaggag
     1981 aaaaatggag tcgaaataaa tatctaaaag ttttaatcga aagtgatttt gaccgtcaac
     2041 ttacgactta caaaaaagac caattcgatt tattgctcga taaatttatt gctatccaaa
     2101 cagaaaatac tcaaatatta agcgagtatg tctacacaaa taatcaaatc attgaaatgc
     2161 ttatcggagg aatgatataa ccatgagaaa aacttctgtc caaaaacatg ttactttgta
     2221 ccctagtcat gttgacgttt tagattccat catcgaaaaa aatccagata tcaataacta
     2281 ctcggaagcc attcgctttt tatgtatgag ttatgaaccc gataccaaca cgaccactac
     2341 cagcaatcag gcaaaactaa atgctatggg aaaagaaatt tccatcatga ctgaaatcat
     2401 cgcagaattt gctgatttat atctaaaaga tacaggcgtt cttattggca caaattctaa
     2461 agtatatcaa gaagtgaaag aaaaaatcga agctaaacta aatccatcaa atcgaaatcg
     2521 accaaagaat catcaaaaag aaaccagtca aacagtcact tctagaaatt tcgacccaac
     2581 aaattttcta aaacgataaa aaacagatag gagttattcc tatctgtttt ttatttagaa
     2641 gccccataca atatgctaga atgtactagc aatcttagtc ggttgtaagg gacttgtcta
     2701 cttgcttaat gtaagggggg cgagttcttt tttatttatt cttctaacca gttacgcaga
     2761 gaaatctttg gcaaatctga cgttaggctt gaaaaagtaa attctaattt ggcgatttta
     2821 ttgccctttt ttgcttttat ttttgtgact ttaagatcat tgaagtattt ttttagttct
     2881 cgtaatgcag gatttaatac tttttgatca atgttaccca tttgatagta ttctgggata
     2941 tccattaagc tcctaaactc ttcaatacga actacataat aaccagtaga acggtactgc
     3001 ataagcagtc ggaatagagt ttttgcatac gtagaacgca acgacgtgaa agcagataat
     3061 tcaaatctac taaattcagc tgttaaacca ttaataatat attctaggtc cggattaaca
     3121 ctgacttcaa caaacttttt tgttttattg atttcaaaac cagtaaacaa aacaaagtag
     3181 cgaatagaat cctcatcttc ag
//
LOCUS       DANKAY010000193         3178 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000193 DANKAY010000000
VERSION     DANKAY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            75..503
                     /locus_tag="PWY98_002942"
     CDS             75..503
                     /locus_tag="PWY98_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2038622.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="PWY98_002943"
     CDS             490..732
                     /locus_tag="PWY98_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038623.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="PWY98_002944"
     CDS             complement(1032..1247)
                     /locus_tag="PWY98_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038624.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="PWY98_002945"
     CDS             1354..1668
                     /locus_tag="PWY98_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038625.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="PWY98_002946"
     CDS             1772..2950
                     /locus_tag="PWY98_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2038626.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1046 a          483 c          638 g         1011 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagt
//
LOCUS       DANKAY010000194         3127 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000194 DANKAY010000000
VERSION     DANKAY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(133..1350)
                     /locus_tag="PWY98_002947"
     CDS             complement(133..1350)
                     /locus_tag="PWY98_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2038627.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(1499..1678)
                     /locus_tag="PWY98_002948"
     CDS             complement(1499..1678)
                     /locus_tag="PWY98_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038628.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1733..1816
                     /locus_tag="PWY98_002949"
     tRNA            1733..1816
                     /locus_tag="PWY98_002949"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1767..1769,aa:Leu,seq:gag)
     gene            complement(2056..2634)
                     /locus_tag="PWY98_002950"
     CDS             complement(2056..2634)
                     /locus_tag="PWY98_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038629.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(2640..3023)
                     /locus_tag="PWY98_002951"
                     /pseudo
     CDS             complement(2640..3023)
                     /locus_tag="PWY98_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1040 a          613 c          490 g          984 t
ORIGIN      
        1 aggaagtcgc aaacttcaag aaaagacttg caactaaata atatacttta gttatttaag
       61 aaaactccac tagaaagaga gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt
      121 tggtttttaa aatcattttt ggcgataggt tgctaggaat tctctcattt tcttagctgt
      181 ttgtttcaaa tcatccattt taggaagata aacgattcga aaatgatctg gctggttcca
      241 attaaatcca ccaccatgta caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa
      301 tttttcatca tccaaaatat tgaatcgctt tgtatcaatt tttgggaata tataaaaagc
      361 agcttttggt tttacggctg aaagaccagg aatatcattg attgcattat agatatattc
      421 tctttgttca tagatccttc cgcctggcag gagcagctca tcgacacttt gataaccgcc
      481 tagcgcggtt tggataattt gctgagacaa tacattggaa cacaaacgca tggaggacaa
      541 catattcagc ccttctatat agcctttgac gtgtgactta tctccactta ggaccatcca
      601 gcctactcga aatcctgcta cacgatgaga tttggataga ccgttcagcg taacaacgaa
      661 caaatcaggc gctaatgtag caatcggaat atgttctaga ccatccatca ctaagcgatc
      721 atagatttcg tcggaataaa taatcaggtt attttgtcga gctacttcaa cgatttcttc
      781 taataattct tttggataaa gtgccccagt aggattatta ggattaatga taacaatagc
      841 ttttgtccga ctcgttattt ttgatttgat atcatcaata tcaggatacc attctgcctc
      901 ttcatcgcaa atataatgaa caggtgtgcc tccagctaat gcaatagaag ccgtccacaa
      961 cggatagtcc ggcattggaa ccagtacttc atctccattg tccagcaacc cctgcataca
     1021 catcgtgatc aattcgctca ctccgttgcc ggtgtagata tcattgatcg tcacattcgg
     1081 aaaatgtttc agttggcaat attgctcaat tgcttttcta gcagaaaaga tccccttgga
     1141 atctgagtag ccttctgagt cacgggcatt catgatcaaa tcacgaatga cttcgtttgg
     1201 tgcttcaaat ccaaaaggtg cgggatttcc cgtgttcaat tttaaaatac taatgccctc
     1261 ttcttgcatc cgctccgctt cttctagaac gggaccgcga acatcgtaac tgacaccttc
     1321 taatttgttg gatttttcaa attctctcat cacttagcca atccctcatt tcaaaaagtt
     1381 agaactttcc aaattttttg ttatttacta tttcaatatc atactccatc ctagaaaatt
     1441 ttgccactta tttaggaaaa acgtaagtat taaaaaaaca caaacaagcg ttgagaactt
     1501 aattgtcatg atagtcattc catagattcg ctgcccaatc cattgcaatt ccacaaatga
     1561 ttcctagcat aaaaccaagc aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga
     1621 tcattcccag tcctgaaaat tctccgtcat atttctccat cttaataagt cctcccattt
     1681 ttgagcttga caactatgta aaacctcgct atcattagtg tgttattatt acgcagatgt
     1741 gttggaattg gcagacaagc atgattgagg gtcatgtgag cgttagctcg tgtgggttcg
     1801 agtcccatca tctgcactac aactcgtgaa gttaattttg cttcacgagt ttttttgttt
     1861 gaacgtttct ttatgccatt atatacatat cactgtatcc tttacaaaaa gaacgtacaa
     1921 tcatgtaaac agcctgaggt gagtatatga aggtcggaaa caagctactc gtagaaactt
     1981 gcaagtgtta aaacaattaa tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga
     2041 gggatttttt gtatattatc tgtttatcac cgttattata aaggtacaaa taccaagcac
     2101 ggttaatcca aattgcataa attcattttt ttgtacactc atgcttttca ttagctcttt
     2161 aactgccatt agtactaaga tggcgataac aacataaagt atatagtacg tccgcattcc
     2221 ggaagcccat actgtgggag aaaatcccat aattaacctt gaacaaaatc ccattatcat
     2281 caaaataact aaaaaaatag aagttttctt attgtcaaaa cttaaataaa ttccaacaat
     2341 taaacatagt aataatgcca agactactaa agtagctatc caagtacctg gataaaaaag
     2401 tgacagtttc gttcctaact ctgtaaagag attattcagg ttactactac tccaaatcat
     2461 tgccctttta tttccattga cgtaagtaaa gctttgaccc attgtattac caaaataaat
     2521 aatcaggttt aagaaaaacg gtaatgcagt caatatacgc acataatagt tttgacattt
     2581 cctatatgtt agaaggaaaa ttaagaaaaa tagcaataaa aataagatat tcatgtctaa
     2641 aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt
     2701 ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt
     2761 gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa cttataatta
     2821 tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg
     2881 ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt
     2941 ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat
     3001 gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc
     3061 aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca
     3121 aaaacat
//
LOCUS       DANKAY010000195         3071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000195 DANKAY010000000
VERSION     DANKAY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            287..1102
                     /locus_tag="PWY98_002952"
     CDS             287..1102
                     /locus_tag="PWY98_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038630.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="PWY98_002953"
     CDS             1228..1485
                     /locus_tag="PWY98_002953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038631.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="PWY98_002954"
     CDS             complement(1737..2309)
                     /locus_tag="PWY98_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2038632.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /locus_tag="PWY98_002955"
     CDS             complement(2325..2930)
                     /locus_tag="PWY98_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2038633.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1018 a          539 c          473 g         1041 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta g
//
LOCUS       DANKAY010000196         3024 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000196 DANKAY010000000
VERSION     DANKAY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            638..>3024
                     /gene="esp"
                     /locus_tag="PWY98_002956"
     CDS             638..>3024
                     /gene="esp"
                     /locus_tag="PWY98_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2038634.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1102 a          456 c          595 g          871 t
ORIGIN      
        1 tatttgagtg aaagcaatta attattcatg tagagtacta gacactttaa atatggacaa
       61 ttcaattata aagaaaaaga atcaataatt taatgaggct gtgacaaatg tcattttttg
      121 tttcgctaaa aacgagtgaa aaaatgttga taactccaat cattcgttta agattctagg
      181 aatcattcag aaatgctgtg aaaccgatac tttataatta taaagaaggt gatttcaaat
      241 tccgaagaag gaatttgaag tcaccttttt acttatgtca cagtctcatt cagtaatgcg
      301 ggataaggct ccttattccg ttatttgaat actctgaagt ttaatctaag aaagggtaat
      361 attttgaaaa catgcttcat cgcatagaaa tgaacttaat ttcagaaaga atacatacat
      421 atctttttat atgttttcag ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca
      481 tgaagaaatg tacttttttt gttgtaatgt tcatgaaatt ctctaagtaa attagttaac
      541 ataaggttag taagattcct attctgtgaa taatacgtat cagatttatc ttttattctt
      601 actaggaaag taattattaa gggagttgat ttgaataatg gttagcaaga ataataagag
      661 agtatttcta gaaaaaacaa agaaacgagt attgaagtac agtatcaaaa aattaagtgt
      721 tggggttgca tctgttttga ttggtgttgg tcttgtcttg ggaacaactg aactagttaa
      781 agcacaagat gaaatttctc caagcagccc acttgaaaca gcgataagct ccgtacaagt
      841 aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat cctggttata cgaaaaatta
      901 taacttttcg gatttacaat ttagtcctca agaactaact ggagacacac taaaaggcaa
      961 tacaattgga tttgaagttt acggaaaaca taatattgca gcatcaacta aaaactggga
     1021 aatccgtctt caattagatg aacgattagc taaatatgta gaaaaaattc aggtcgatcc
     1081 aaagaaagga attggatctt ctagacggac ctttgtaaga attaatgatt cgctgggtag
     1141 acctacaaat atttggaagg tcaattatat tcgtgcaagt gatggattat ttgctggggc
     1201 agaaacaacc gatacgcaaa ccgctcctaa tggggtaatt acgtttgaaa agagcctaga
     1261 tgaaattttt aaagagatag gcatagacaa tctcaagacc gatcgtttaa tgtatcgaat
     1321 ctacttggta agtcatcaag acgatgataa aattgtaccg ggtattgata gtacgggcta
     1381 ctttttaaca gattcagatg atttctataa tagcttagat gtatctgaaa ataatcctga
     1441 ccagtttaaa catggatcag taagtgcaaa atatgagaaa cctaatactc aaacaaaaga
     1501 tggttcagga tccacaggtg ctaatggagc tataatctta gatcataagt taacgaaaaa
     1561 ttataatttc tcatattcat cttccgcaaa gggcacgcct tggtatgcta attacaaaat
     1621 tgatgaacga ttagttccat atgtagcggg tatacagatg cacatggttc aggctgacaa
     1681 agtaacatat gatgtttcgt ttgaatccgg aacaaaagta gctgatttag caatcgagcg
     1741 acgggaaggt catgaaaatt atggcatagg ttcaataact aataatgatt taactaaact
     1801 tatcgatttt gctaatgcaa gtccacgtcc agtcgttatt cgatatgttt tacaattgac
     1861 taagccatta gatgaaatct tagaagatat gaaagcaaca gcacaagttg aagaaaataa
     1921 accatttggt gaagatttca tctttgattc ttggttgtcg gatacgaata aaaaattaat
     1981 ccagaacact tatggaacag gttattatta tttgcaagat attgatggtg atggaaaccc
     2041 tgacgataaa gaagagagcg gagacacgaa tccatatatc gggaaacctg aattagaaga
     2101 agtatatgat gttgacacaa cagttaaggg gaaagtattc atccacgagt tagcgggaac
     2161 aggtcacaaa gcccaacttg ttgataaaga aggtactgta ttagcagaaa aaactatcgc
     2221 tccaaatgaa aaagatgggg ctccaatttc agatactgta gaatttgaat ttacgggtgt
     2281 agattcaagt aaattaatcg cgaaagatga attaaaaatc caaatcgttt ctccaggttt
     2341 tgataaacca gaagaaggtt caaccgttat taaggaatca ccaaaagcgg ttgataaaca
     2401 aaccgtggta gttggattta aaccagatgc taaagaatca attcggaata ataaaaactt
     2461 acctgaagat gcagagtatt catggaaaac agagcctgat acttctaacg ttactgatag
     2521 tacgaaaggt attgtaactg ttaagatcgg aaatcgaact ttcgacgtgg atgtagagtt
     2581 tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca tacgtaccta taacaacaac
     2641 tccagaaacg acagttcaaa gtggaaaacc tacatttgat aaaccagatg ttcctttagc
     2701 taaagatgcc ttttcaattt cagatgtcta taataagtac ttcggtaatg caagtgttga
     2761 tgcaaacact ggtattgtta cattcactcc agctaaaggt gtaggagaat cggagccgat
     2821 tactggaaca attcctatta aaattgttta ccaagatggt tctgtaggca cgaccgattt
     2881 agcagtaact gtaagtaaag atatttatga aaatccagga gaaaacattc ctgcaggcta
     2941 ccacaaagta accttcaccg caggggaagg aacaagtatt gaaagtggaa caacagtctt
     3001 tgcagtgaaa gacggcgtaa gctt
//
LOCUS       DANKAY010000197         2930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000197 DANKAY010000000
VERSION     DANKAY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            182..2416
                     /locus_tag="PWY98_002957"
     CDS             182..2416
                     /locus_tag="PWY98_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2038635.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2564..2884
                     /locus_tag="PWY98_002958"
     CDS             2564..2884
                     /locus_tag="PWY98_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2038636.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         1040 a          539 c          595 g          756 t
ORIGIN      
        1 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
       61 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
      121 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
      181 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
      241 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
      301 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
      361 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
      421 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
      481 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
      541 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
      601 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
      661 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
      721 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
      781 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
      841 agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
      901 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
      961 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
     1021 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
     1081 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
     1141 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
     1201 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
     1261 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
     1321 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
     1381 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
     1441 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
     1501 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
     1561 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
     1621 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
     1681 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
     1741 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
     1801 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
     1861 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
     1921 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
     1981 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
     2041 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
     2101 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
     2161 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
     2221 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
     2281 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
     2341 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
     2401 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
     2461 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
     2521 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
     2581 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
     2641 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctc aacataatga
     2701 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
     2761 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
     2821 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
     2881 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag
//
LOCUS       DANKAY010000198         2782 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000198 DANKAY010000000
VERSION     DANKAY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(243..392)
                     /locus_tag="PWY98_002959"
     CDS             complement(243..392)
                     /locus_tag="PWY98_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038637.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="PWY98_002960"
     CDS             854..1066
                     /locus_tag="PWY98_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038638.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..>2782)
                     /locus_tag="PWY98_002961"
     CDS             complement(1344..>2782)
                     /locus_tag="PWY98_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2038639.1"
                     /translation="VRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIMDFIFA
                     NRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLLAITGQ
                     FIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNIVMSTV
                     GIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCLYNMDY
                     LTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGHFVVMH
                     DANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKLLIEIK
                     TSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILPFNIVG
                     PPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGIITDEM
                     GLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          973 a          480 c          491 g          838 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tt
//
LOCUS       DANKAY010000199         2724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000199 DANKAY010000000
VERSION     DANKAY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            172..516
                     /locus_tag="PWY98_002962"
     CDS             172..516
                     /locus_tag="PWY98_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2038640.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="PWY98_002963"
                     /pseudo
     CDS             517..936
                     /locus_tag="PWY98_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="PWY98_002964"
     CDS             complement(1028..1378)
                     /locus_tag="PWY98_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038641.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="PWY98_002965"
     CDS             complement(1344..2675)
                     /locus_tag="PWY98_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2038642.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1011 a          491 c          525 g          697 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agat
//
LOCUS       DANKAY010000200         2680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000200 DANKAY010000000
VERSION     DANKAY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            11..1657
                     /locus_tag="PWY98_002966"
     CDS             11..1657
                     /locus_tag="PWY98_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2038643.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1648..>2680
                     /gene="uxuA"
                     /locus_tag="PWY98_002967"
     CDS             1648..>2680
                     /gene="uxuA"
                     /locus_tag="PWY98_002967"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2038644.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYL"
BASE COUNT          875 a          481 c          566 g          758 t
ORIGIN      
        1 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
       61 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
      121 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
      181 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
      241 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
      301 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
      361 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
      421 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
      481 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
      541 tattcttgct gcgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
      601 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
      661 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
      721 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
      781 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
      841 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
      901 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
      961 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
     1021 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
     1081 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
     1141 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
     1201 tccaaatatt cctgatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
     1261 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
     1321 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
     1381 aggaaaacca ttcatcccga gtccagaccc actattgaca gactttcagc ataagctttc
     1441 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
     1501 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
     1561 agaaatgttc atcaagatgg tagcaggacc gaatgccgta cgaaaaacat tacaggatta
     1621 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
     1681 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
     1741 acattgatgg atataccagt aggagaggta tggcccaggg agaggatcaa agaactaaaa
     1801 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
     1861 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
     1921 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
     1981 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
     2041 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
     2101 gaatttgaat tgccgggatg ggaacctgag cgcttagctc atatcaaatc cttgcttgaa
     2161 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
     2221 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
     2281 tactcgattt ttggattacc tcgaatcgta aagaatcgag aagatttaga ttggttgtgc
     2341 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
     2401 ccagaaaatg atgtatatgg gattttagca gaattttgtc aaagagatcg aatcccattt
     2461 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accgcataaa
     2521 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt
     2581 aacgaataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
     2641 tacggcttat atgatcgagc attaggtgct gtttatctaa
//
LOCUS       DANKAY010000201         2634 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000201 DANKAY010000000
VERSION     DANKAY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            6..1150
                     /locus_tag="PWY98_002968"
                     /pseudo
     CDS             6..1150
                     /locus_tag="PWY98_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="PWY98_002969"
     CDS             1321..1779
                     /locus_tag="PWY98_002969"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2038645.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="PWY98_002970"
     CDS             1938..2408
                     /locus_tag="PWY98_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2038646.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="PWY98_002971"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="PWY98_002971"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          863 a          504 c          497 g          770 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DANKAY010000202         2619 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000202 DANKAY010000000
VERSION     DANKAY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(1..317)
                     /locus_tag="PWY98_002972"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="PWY98_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(391..672)
                     /locus_tag="PWY98_002973"
                     /pseudo
     CDS             complement(391..672)
                     /locus_tag="PWY98_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            1134..2516
                     /locus_tag="PWY98_002974"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="PWY98_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DANKAY010000203         2613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000203 DANKAY010000000
VERSION     DANKAY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            316..570
                     /locus_tag="PWY98_002975"
     CDS             316..570
                     /locus_tag="PWY98_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2038647.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="PWY98_002976"
     CDS             571..888
                     /locus_tag="PWY98_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2038648.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="PWY98_002977"
     CDS             1447..2382
                     /locus_tag="PWY98_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038649.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2476..>2613
                     /locus_tag="PWY98_002978"
     CDS             2476..>2613
                     /locus_tag="PWY98_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2038650.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          958 a          375 c          483 g          797 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcataga gtagtctcaa aactcccaag
     2461 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
     2521 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
     2581 attaaaaacc gaagaaatac tgatcaactg aag
//
LOCUS       DANKAY010000204         2595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000204 DANKAY010000000
VERSION     DANKAY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            86..2527
                     /locus_tag="PWY98_002979"
     CDS             86..2527
                     /locus_tag="PWY98_002979"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2038651.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          866 a          461 c          611 g          657 t
ORIGIN      
        1 atttaatctt agaaaaaaat aactggaaca agagctattt gtttttacag caaagaatgg
       61 tatatttttg taggaggtgg cttacttgaa agaagtcgtt atgatagatg ctgcaagaac
      121 gccgatagga aagtatcgcg gaagtttaag tccatttact gcagttgaat taggcacact
      181 tgtgactaaa ggattattag ataaaacaaa actgaaaaaa gataaaatcg atcaagtaat
      241 atttggaaat gtcctgcaag ctggtaatgg tcaaaatgtt gccagacaga ttgctttgaa
      301 cagcggtctc cctgtagatg tcccagcaat gacgatcaat gaagtttgcg gatctggtat
      361 gaaagcagtg atcttagcac gccagctgat ccaattaggt gaagcagaac ttgtgatagc
      421 cggcggaaca gagagtatgt ctcaagcacc tatgctgaaa cagtatcagt cagaaacaaa
      481 tgaatatggt gaaccaattt ccagtatggt caacgacgga ttgactgacg cattttcaaa
      541 tgcacatatg ggattaaccg cagagaatgt tgcaacacaa ttttctgtga gcagagaaga
      601 gcaggatcgc tatgccttgt cgtcccagtt gaaagcagca catgctgtcg aagccggtgt
      661 attttctgag gagatcatcc cagtcaagat ttctgatgaa gacgtgttat ctgaggatga
      721 agcagttcgt ggaaatagta cattggaaaa actgggcacg ttacgtacag tattctcaga
      781 agaaggaact gtaacagcag gaaatgcttc cccgttgaat gacggtgcct ctgtggtgat
      841 ccttgcatcc aaagaatacg cagaaaataa taatctgcct tatttagcaa ccatcaaaga
      901 agtagcggag gtcggtattg acccaagtat catgggaatc gctccaatca aagccattca
      961 aaaattgaca gatagatcag gaatgaattt atcaacaatc gatctttttg agatcaatga
     1021 agcatttgca gcttcttcta tcgtagttag ccaagaactg cagttagatg aagaaaaggt
     1081 caatatatat ggaggagcaa tcgcattagg ccatccaatt ggtgcaagcg gtgcacgtat
     1141 cttgactaca ttagcttacg gattgcttcg tgaacagaag agatacggta ttgcttcatt
     1201 atgtattggt ggaggtttag gcttggcagt cttgttagaa gcgaatatgg agcaaacaca
     1261 taaggatgtg caaaaaaaaa agttttatca gcttacgcca tctgaacggc gcagtcaatt
     1321 aatagaaaaa aatgtattga cacaagaaac tgcgttgatt tttcaggaac aaacactttc
     1381 tgaagagctc tccgatcaca tgatcgaaaa ccaagtgagt gaagtggaaa tcccaatggg
     1441 gatcgcgcag aattttcaaa tcaatggcaa gaaaaaatgg attccgatgg caacagagga
     1501 accatctgtc attgcagctg caagtaatgg tgcaaaaatc tgtggaaata tctgcgcaga
     1561 aacacctcag cggctgatgc gagggcaaat cgttctatca ggaaaatcgg agtatcaagc
     1621 agtgatcaat gcagttaatc atcgaaaaga agagctgatt ctttgtgcaa atgaaagtta
     1681 tccttcaatc gtgaaacgcg ggggcggtgt tcaagatatc tctactcggg aattcatggg
     1741 gagtttccat gcatacttat ctattgattt tttagtggat gtaaaagatg caatgggcgc
     1801 taacatgatc aactcgattc ttgagagtgt ggcaaacaag ttacgggagt ggttccctga
     1861 agaagagatt ctattcagta ttttaagtaa ttttgctaca gaatcattgg cttctgcctg
     1921 ttgtgaaatc ccattcgaac gcttagggag aaacaaagaa atcggtgaac aaattgccaa
     1981 aaaaatccaa caggcagggg aatatgcgaa gcttgaccct taccgtgcag caacgcacaa
     2041 caaagggatc atgaatggta tagaagctgt agtggcagct acaggaaatg atacacgtgc
     2101 ggttagcgct tcgatccacg cctacgcggc aagaaatgga ttataccaag gattaacaga
     2161 ctggcagatc aaaggggata aactagttgg aaaactaacg gtgcctttgg ctgttgccac
     2221 agtaggcgga gcttccaata tcctgccaaa agcaaaagca tctttagcta tgttagatat
     2281 tgattcagcc aaggaactag cacaagtaat tgctgcagta gggttagctc aaaacctggc
     2341 tgctttgcgt gcattagtta cagaaggaat ccaaaaaggc cacatgggac ttcaagctcg
     2401 ttcattagcg atctcaattg gggctattgg tgaggaaatc gagcaagttg cgaaaaaatt
     2461 acgtgaagca gaaaagatga accagcagac agctatacaa atattggaaa aaatccggga
     2521 aaaataaaaa agcaatatct ttagaagaat aaagcaagat ttacagttat tttatttact
     2581 gcagatcttg ctttt
//
LOCUS       DANKAY010000205         2497 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000205 DANKAY010000000
VERSION     DANKAY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(112..1050)
                     /locus_tag="PWY98_002980"
     CDS             complement(112..1050)
                     /locus_tag="PWY98_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2038652.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1541..2497
                     /locus_tag="PWY98_002981"
                     /pseudo
     CDS             1541..2497
                     /locus_tag="PWY98_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
BASE COUNT          859 a          431 c          398 g          809 t
ORIGIN      
        1 ttttaaattt ttatatttag gaggaataat cctctgattt tttcatacgt tatgtcacct
       61 cgtaaatatt aattatactg aattagcaat ttttatcaaa taaaacttat tttacttcca
      121 aaacctaaat tcacgttgcc aaaaatcaat ctgcttttgc aattgttttt cgttcgcttt
      181 taaagtcgat ttcattaatt ccgttaaatc aattggagat atttctctaa tcaatttttt
      241 aaatttagtc ttagtattct tacttagctt tccccacata ctttcttcat gcaacaaagt
      301 ataaaccata gcttgctcat taattttttc taaagtagcc cacgcaggtt tcaagatgtg
      361 taaatcatta aaacaatcat tccagtaatc aaccatatct ctttttaatt caacttctac
      421 acgccataaa tgttcagaca caacttcaac atctgcgtta tctttacgtt cttgtttttt
      481 attataaatt ctaataaatc tatcactatc acggacacca aaatattttg tttctggctt
      541 gccattacga ccataaaaaa cagttttctt aactgcttta tcagtcattg catagtaatc
      601 gctcaaatca tcttcaaaat caaaagctaa gtctaatctt gtaaaaccgt catcttccat
      661 gtagtcgata atattttgtt ttaaccaaat catttcttca tgtgtgagtt tattgggatt
      721 aaattcaaca cgcatattac gtctatccca agtatctgct tttactttgt catattcgat
      781 ataaactttt tcttgaagtg ccttagcttt aaactttgtt tgaagtatat cccaaagtcg
      841 tatttgtggc tctacactca taaagtcaga tagcttttta gcattagttt tgttcaaatt
      901 tccaacgatt gtcatggcat caaaacttaa tgcgggttga gattttccca aagtttgacc
      961 acttaaccgg ctattactta accggctatt agagacggcc ggttttttta gtgagatttc
     1021 tgcagctaat ttttcatcaa ttttcttcat tttttatcgc tcctaaaata atttttggaa
     1081 cgaaaaaagg acgagtggaa tcgcaaaata aatttcgcct gtatccattc gtccattttg
     1141 ttttgtattc aaaatcaaaa attgatatac taatcatcga atgaataaca agtgtggttt
     1201 gttattccgg gcgagaacgt tggtagcgtt gctcgtcttt ttttatgtct ttttttcgtt
     1261 aaaactattt taaaactaaa tatatcttaa agcaaataaa ttcaaggggc taaaagcgcc
     1321 ccttaaaaac cccttgcctc tgaaaagttt tgtgtttcct ttccgtcggt ttcaaaagct
     1381 aatttactca aaaaaataga tcaagggtaa atgcactcac tgcgttcgcc cttgagctat
     1441 tttatttcgc aaattttaac gcatttgccg acgaaaagat aaaacacata aaaatatacg
     1501 cagatatatt taaaacgtct tagaacgcaa aattttacgt ttaaaagcta tgcaaggact
     1561 gtatgaagca aaattattga gttatccaag aacagataca ccatttatta cagagaacga
     1621 atttgcttat ttaaaagcga attttggcaa atatagcggt tttttaggac ttgatcttga
     1681 aatggttcaa acagagccta gaaagcgtta tgtggacggt agtaaggtac aggaacacca
     1741 cgccattatc ccaacaaaac aagtacctac cgaatctgca ttagcgaaaa tggacgattt
     1801 acaacgaaaa atttatgctt tagtcgttaa aacgaccgtt gccatgtttt tacctgatta
     1861 cttgtatgaa gaaaccaaga tacaaacgaa agtagctgat ttgctgtttc aatcgatcgg
     1921 aaagacacca aagcaagaag ggtggaaaat tcttttcaaa caacaaacca aagaagaaaa
     1981 agaggacgtt caaacgttac cactcgttat cattggcgaa cgtgccgaag ttggtgttaa
     2041 gagtgttgaa aaagaaacgc aaccgccaaa agcttttaca gagggtacat tattaactgc
     2101 tatgaaaacg gcgaataaaa cggttgatga tgaagaagca atcaagattt tacaagaagt
     2161 tgaggggatt ggaacagaag cgacaagagc aagtattatt gaagcgttaa aacaaaaaga
     2221 atatatccaa gtgattaaga ataagcttgt tgtaactgaa aaaggaaaat tattgtgcca
     2281 ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat gggaaacgta
     2341 tttaaaaaaa atcggtaaaa gagaaggcaa tcaagagaac tttattacga atatcaaaaa
     2401 attcattgtt catttactgg aagctgtacc taacgatata gaaaaactaa atttttctga
     2461 ttaccaggaa cagaaagaaa aagaagcaga aaaaagt
//
LOCUS       DANKAY010000206         2487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000206 DANKAY010000000
VERSION     DANKAY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..348)
                     /locus_tag="PWY98_002982"
     CDS             complement(<1..348)
                     /locus_tag="PWY98_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2038653.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGV"
     gene            complement(367..1167)
                     /locus_tag="PWY98_002983"
     CDS             complement(367..1167)
                     /locus_tag="PWY98_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2038654.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1391..1606
                     /locus_tag="PWY98_002984"
     CDS             1391..1606
                     /locus_tag="PWY98_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2038655.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1745..2230
                     /locus_tag="PWY98_002985"
     CDS             1745..2230
                     /locus_tag="PWY98_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038656.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          714 a          483 c          387 g          903 t
ORIGIN      
        1 gacaccgact tcttcagaca aatcatccat acgtaagaaa tcctctgttt cgatcagcct
       61 atttagtaaa taagcgattc ttttggatgc cgaattgaaa tcactggctg ttttcaattt
      121 tccttgcatg atctcatcaa aaaaatcaaa atcataaatt tcaagttcaa aatgtttgtt
      181 tttttggatg atttttgcaa taccggcaat ttcttcattc aactccccaa tatctttttt
      241 tatggtttga ttactggttt tgatttcttt ttgcacttcc tggattgaaa ctttcttgtg
      301 agtgactagt aatttcaata attggtacca tctgtttact agcgtcacgt gcgtcccccc
      361 tatcctttat cctcttgttt tacctcctga aatattagtc gtgactccag tgatataact
      421 tgatctttct gaaagataat agcagactag atccgccact tcttgcagct taccactcct
      481 tcctagaggg atcgtacttg ttcgactgta accgtttctc agctgttcga cgcttatacc
      541 tctcgtataa gaaagagctt cttcatattc ttgtgttctc aaaccggttt cttccagtat
      601 ccctggtgca acacctacca cacgaacatt cctctttcct aactcttttg cccaagaacg
      661 agtaaaactg ttcatcgctg cttttgtagc agcataacag ctctgtcctt ctgatccttc
      721 caatcctgat tcagaagaaa gattcacgat cactcctgat cccttttgca ccaagattct
      781 tccaactgct tggctcatca aatatacact tttttgattg acagcgatca tcttatcaaa
      841 aacttgttct gataattcat acttgctttt tggatgattt ctatctatta gaagagaagg
      901 aatatttatt cctgcattat tgactagacc atcgatcgtc ttgaaatgtt tcattacttt
      961 atatatccct tcttctatgt ccgttttaga agacacatcg acttttaaag atagtaggtt
     1021 ttcatgctgt atacggcatt cagttacatc aaaattcgct acttgtgcat tctgcttcaa
     1081 caaattttcg acgatacttc ttccaattcc tgaggaacct ccagttacaa ttactacttt
     1141 tcctgcaata tccaaccaat ccgacatatg tgatcctctc cttctttttt cttacaaatc
     1201 cagtataaaa gctcactaaa aaaaattaag acatgaaatt tacagcttca gctaaaaaat
     1261 gtgtactaat ggaagagcaa aaaaaatttt catctttttg catagattgc taaaattcct
     1321 gagcgttata taagcaatgt ttctcttttt gatatataat tgttttatca tttaagaaag
     1381 gagaattcta atgaaaactt tagatgctat tgcattaact ttattgattg tgggagggtt
     1441 gaactggctg ttggtaggct tgttcgaact tgatttagta gctatgattg ctggtggttc
     1501 aacaactatt tttgcgaaga tcatttatat cgtcgtaggt atttgtgcga tctactgctt
     1561 gaaatttttc ccaatgatta ctagaaaagt tgatgaaaga tattagaaag ctataattat
     1621 taaaaaagtt attatcttca tgaaccttcc ctttattggg aaggtttttt cattttaaac
     1681 catctgcttt tctttgttta agtgatatac tgaataaaga taatctattt taggaggagc
     1741 tatcatgcat aaagggattt acgcaggctt gttgggaact gtaatacttt tttctgcctg
     1801 tggatataaa acaaagaatt ctgttaaaga atcttcagca gccatttcaa caactgaggt
     1861 tggttcaact aaaagtactt ctactacttc cattactaat acttctactg gtaatgtaga
     1921 ttcgcaacat agccaatcaa gtattcagtc gtttagcatt tcttcaacat ctgaaatgca
     1981 agaaagcact gtaccttctc ttccattaga aaactctgac aaagcacttc tatttcttat
     2041 tgcccaccgt tccgaattac aaagtgacga tattgttatt agcttttatc aaaaaattga
     2101 tcgagattat ttgttcacag cttccagcaa acaaatccgt tcccaaggag gatctggaag
     2161 cgttggtttt tatcgagtgt cacccgaagg atcgattacg atgacagatg caaatggaac
     2221 accattttga ttagatgata ttatacggat ttacagtact agcaagtcat tattggtatc
     2281 attttttctg agaaagttat acggagtata aataagcatt tgcattaacc taataattcg
     2341 ttacagctat ttctcttatg aatatactca cttctctact tgctctcttg ataaattttt
     2401 ggtatctact tcattttcgg tcaactgagt taatgaaatt tattgtcggg cggttttcta
     2461 gattcaattt ttctatattt ggataga
//
LOCUS       DANKAY010000207         2431 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000207 DANKAY010000000
VERSION     DANKAY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..537
                     /locus_tag="PWY98_002986"
     CDS             <1..537
                     /locus_tag="PWY98_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2038657.1"
                     /translation="HDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHG
                     SQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGP
                     DLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSD
                     KLLNRIDKEILKVGNIDE"
     gene            530..1957
                     /locus_tag="PWY98_002987"
     CDS             530..1957
                     /locus_tag="PWY98_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2038658.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1959..>2431
                     /locus_tag="PWY98_002988"
     CDS             1959..>2431
                     /locus_tag="PWY98_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038659.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKID"
BASE COUNT          853 a          286 c          387 g          905 t
ORIGIN      
        1 catgattatc ctagaattaa taggaataac acaactgcat ttaaaatttt tagagctata
       61 attaatcgtt gtctgaggat gattaaactt cccccaaaaa ttagacacca taaatttgat
      121 ttgtatcatg gttctcaatg gtgggctttg tcgaaagaat gcctaacaga attaatacaa
      181 atgtatgaac aaaatcaaca agactacctg aattttaaaa taggtatgtt tgccccagat
      241 gaaaaatttt ttcatacact tttttttaat tcctcgttca aaaataaaaa tgttattgga
      301 ggaccagatt taccacttga attgaaaaat attgaagaaa ctactttaca aacttcgaaa
      361 ttagcaaata ttcatattat tgatccttcc atgaacaaat ggtttgatga aagggatata
      421 gatcaaataa aagaaagtga aaaattattt gtaagaaaag ttaaatcgga ttactctgat
      481 aaactattaa atagaattga taaagaaatt ttgaaagttg gaaatataga tgaatagaat
      541 agtacctgtt ttagagaaga gtttattaag tcgagatgcg tttcgagtaa taaatttaat
      601 catttgtctg ctttatttta tgcctagtac ttttaatgta actaatatcc cgatgaaaat
      661 agcttttgct tggggtggaa ttatagtttt gtatgaggtt tttataaaga aaaatttttt
      721 taaaatgcgt ttttcttggc tactgattgg agcaatgatt agttttatct taactataat
      781 cattaacaga gaaaattttt taattccatc tatttataat tttggatatt tgctgattac
      841 tttgatagta atttttcctg cagacttttc acaaagtgaa gaggaaaaaa agaaaaaaat
      901 ggtgtatttt aataatgttt tcattatcat aattttttta gctgcgctaa tatctatcta
      961 tcaatttatt tttttgattt cttatcatgt cccaacagga acgccaggat tacttgcaag
     1021 acaaggtttt attgaacatc ggttatttgg tgtctataca agtccaaatg ttggagctat
     1081 gtttggatat acttctatta ttttaagttt aattagtgta ttaatttcaa aaataacaaa
     1141 aacgattcgt gttttttata tgatgaattt tatcgtacaa atattttata ttacactttc
     1201 ttcgtctaga ggtgcccagg tagtaatttg ttctttttta gtattatttc ttttgttgtt
     1261 tgccaattca aaatttagaa agagcattag agaagtacta cctgtaaaaa tcaaatggat
     1321 ggtaagcata ttattagttc tattggtata tttttctttt ggtactaatt atgctaagga
     1381 ttttttagca acaattcctg tatcgattga tagaaaagta gaagttatag agtcaaatga
     1441 atcagataaa actccacttc cagaaaaaga agtaatatta cagcattcta gtgataactc
     1501 cgaaatttca gctggtcgtt ttacgatttg gaaagctgcg cttaaagtta taagaccaac
     1561 tttgttattt ggttatggag agaccgattt ctatgggaat agcgatgcaa aatttttgaa
     1621 aaaagtagat cttgatacta cagatgtgaa tgaactaaaa agagcacatg gaaacatgca
     1681 taatggattt cttcaagtac ttgtatcaag tggtataatt gcttttatat gtatctatgc
     1741 tttctatgtg ttaaatatct ttagtttact caaaacattt tttagaagta aagtgaatta
     1801 tgcgtttctt ggtattgttt taatttttat actatctatt tttatagatg aaatggtgga
     1861 ggctcatgta cttttcaata agcgcgatgt catttcaatt gttttctggt attatttggg
     1921 aagcatttct tacttgtatt taaaaaagga gaattaatat gaagacagct ttcattgctt
     1981 ggactccttt acatttgatt aatatattaa atgtttatca aaatttttac cgagaatcaa
     2041 aaggagattt atttatttat tctgaatttg aaaacgctag aagatatagt gagcgtataa
     2101 aagaattagg gctatttaat gatgttgttt ttgtgaatca ccggaaaatc ggttcttcct
     2161 ttaaacgtaa aattaacttg ttaactaacc ataatcaatt tttctcggat gatttatccg
     2221 tttatgatga aatttttata cagggggaaa attatttcac aaaaatactt ttctccaatg
     2281 tttacaaaaa aaatccagat gtaaaactaa attatataga agatggtttg ggtacttatt
     2341 tgaagaagag tatattttca acaagtaaaa acgttcaaag agtttttaaa atattaaata
     2401 aaaatagcat cttcaaaaaa aaaattgacg a
//
LOCUS       DANKAY010000208         2398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000208 DANKAY010000000
VERSION     DANKAY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            207..2069
                     /locus_tag="PWY98_002989"
     CDS             207..2069
                     /locus_tag="PWY98_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2038660.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="PWY98_002990"
     CDS             2069..>2398
                     /locus_tag="PWY98_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2038661.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DANKAY010000209         2380 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000209 DANKAY010000000
VERSION     DANKAY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(256..624)
                     /locus_tag="PWY98_002991"
     CDS             complement(256..624)
                     /locus_tag="PWY98_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2038662.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            774..1256
                     /locus_tag="PWY98_002992"
     CDS             774..1256
                     /locus_tag="PWY98_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2038663.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1293..2303
                     /locus_tag="PWY98_002993"
     CDS             1293..2303
                     /locus_tag="PWY98_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2038664.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          837 a          366 c          472 g          705 t
ORIGIN      
        1 gatattttct cgtatttttt ccttattcaa aagttttgca ggaaaattgc tgaaaaaata
       61 attttactaa gtagaaaatt caaagccatt caatcagtga tttttttcag cacaagaaag
      121 actccttata aaaatagcag atactttgtc aattaagtgt ttaaccaggg atgataaaaa
      181 ggataggatt gaacaaaacc tgtataaggt gatgaatagt ttcctcgcca gtaaaaggtt
      241 tattcgtata aagctttatc gccaaaattt atcatgacct tttttatctt caatattaaa
      301 tgaaatcaat tgttcaatca attcccagtt gattacactt ttccaaggaa ctttgatgac
      361 gttatccgta tatacgtagc ctgcttcttg gatatttttt tcaagggcac gaatcgctgc
      421 tttttccggt gcgatagaaa aatgattttt agcaaaacta aatgcaataa taaaggtacc
      481 atggtcagta aacataggtt ggttccattt tacagttgtg tctagctggg gatacgtttc
      541 tgttatctta gcaaaaaggt ctttgatctt ttgttgatgt tctttttctt tgatttgatt
      601 gatgatcggc tgaaattttt ccattctaaa acgcctccat taagtggttt gatttatttt
      661 accagaaaaa caatagttat gtataaaaaa cgaacaaaac acaagtaatg ttgtaaccgc
      721 tatatttata tgataagatg tctcaaataa taaaatagaa aagagacata aaaatgattg
      781 acttggcaaa gaaattctta cacgaaaata tatattttga gttgtatcat catcgagcca
      841 tttatacaaa tgaagatgct ttggtaataa agaaagaaca aggatttaca ggtacagaga
      901 ccaaaagctt attcttaaaa gataaaaaag ataataacta cgtatatctg acttttacta
      961 cgaaacaaac ggatttcaaa gcattaggga agatcgtcgg taaaaggctt tctattgttt
     1021 catcagaacg catggaagaa caaacgggac aaaagcaggg cgctgtttct ccgtttggtt
     1081 atgaaaaaca gattccgatt attattgatg aagagctctt atcaaatgaa aaacttgttt
     1141 ttgcaccagg tagaccagat caaacgatgg tcatccaaac ggttgactta aacaaaatca
     1201 tccagctact tgagatgccg atatttttga tgccggcaaa cgaaaaaaac aattaaccaa
     1261 gtaaaatagt gcatacttgg aggtgaagca acatgataag ttatccatta ttagaaagaa
     1321 atgcaacgat tggggttact gcaccatcct caggcgtatc agaggaacac cattcgttac
     1381 tcgaatcagc tttaaagaga atggaagaaa aatctttcca agtagttact ggaaagacgc
     1441 tttggacgca agataaggaa aaatcaggtc cagcaagaaa acgagcagat gaattcaatc
     1501 aaatgatgca agatgaaaat atccaaatga tctttccgac ttggggagga gaattattga
     1561 ttgagattct cgattttgtc gattttgatg ctctacaacc aaaatggata gtaggttatt
     1621 cagatattag tacgttgcta cttgctgtca cattaaaaac agggatagcc actgcacatg
     1681 gaactaacct tattgattta agaggagagt acttggatga aacgacagca atgtgggaaa
     1741 gtgtactgaa aacaagagaa ggagaatcga ttctacagca atcttctgaa aagtatcaaa
     1801 aagtgtggcc ccatgagcat tcatcatctt ctgttttcca tctcactgaa ccaacaaatt
     1861 ggcaatcagt cagcggagat gcagtaagga tgaagggacg attattaggt ggttgtattg
     1921 atacgatcag gcatttaatc ggtactcctt atggagatgt ccaatctttt aaaagaaagt
     1981 ataatgatgg aaagccgatc ttgtggtttc tagaaaactg tgagctatcg tcagttgatt
     2041 taagaagatc acttgttcag atgaaactgg caggctggtt tgatggcagc tcggggatca
     2101 tgttcggtag aagtgcggca gaggatttgt tagattataa aatgaaggat gtatacgaag
     2161 atctgtcaaa ggaattaaaa ctaccgatct tgtatgatat tgattgtggt catgtaccgc
     2221 cacaactgac attgattaat ggtgcctatg cagaagtagc ggtagaagat agaaaagcga
     2281 ctgttttgca aacgtttatt taaaaaattt tttcctgcct tggagtctat caaagaccaa
     2341 taaatctaaa tagaaaagaa agtgaaagaa gctgacaaaa
//
LOCUS       DANKAY010000210         2295 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000210 DANKAY010000000
VERSION     DANKAY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            10..2148
                     /locus_tag="PWY98_002994"
     CDS             10..2148
                     /locus_tag="PWY98_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2038665.1"
                     /translation="MKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTL
                     FVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAIT
                     YTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKE
                     KQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQEL
                     AVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSR
                     NDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQ
                     SKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENAL
                     GTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVAL
                     DAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTT
                     GIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTV
                     ANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVH
                     GTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVV
                     LPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          786 a          413 c          509 g          587 t
ORIGIN      
        1 aaagatttta tgaaaaaaat attaaatact tcttggttag aaaaaataag aaaaggtgca
       61 caagaaccta agaacaaaac gtttcgccgg tcccatgttc cttttcgctt aaattttctt
      121 tttttcatta tatttacgtt atttgttgcg ttgattgcta gattgggtta tttgcagatc
      181 gtgaacggcg aagagatgga ggcgcgagtg aagtctactt ctacgattac ggtccaagaa
      241 agtactcctc gcggaatgat ctacgactcc acaggaaaag ctttagttac gaataaagcg
      301 aatcaggcca tcacatatac gagaggaaca caagttacgg ctaaagattt gcttgaaatc
      361 gcaacgaagc tgaactcgct gatcgatgtt ccggtagatg aaaatctgac agatagagat
      421 aaaaaagatt tttggctggc tgatcccaaa catttgaaag aagccacaaa aagactttcg
      481 gcaaaagaaa agcaattagg gaacagcgaa caatatagtg cgatggtcaa taaagtcaca
      541 gctgatgaga ttaatttaaa cgaggagcaa ttaaaaattg ctaccatctt taaacggatg
      601 aatagtgcat atgaattaaa cactgtctat atcaaaaact ctggtgttac tgatcaagag
      661 ttagctgttg tggcagaacg cgcttctgaa cttccaggag tttcgactgg cacagactgg
      721 acacgagaat ataatgcagc aagttcactg aaaagtattc ttggcagtgt gacgactgaa
      781 aagcaaggac ttccagcaga tgaagcagag aaatacctag caaaaggtta ttctcggaat
      841 gaccgtgtag gacaaagtta tttggaaaaa caatatgaag atgtcctgca aggaaccaaa
      901 acccaatatg aagtttccct tgataatgaa ggaaatgttt cgaatcaaaa agaaatcttc
      961 tctggggaaa aaggttctaa tttaatgtta tctatgaatg cagaattcca atcaaaagta
     1021 gaagaaattt tgaaaaggaa ttatcaaaca ttgatcaaca acggcaaagc acagtattcg
     1081 cctggtgctt atgcagtggc tatgaatcct cagacaggag aagtgctagc aatgactggt
     1141 ttcagtcatg aacaaggttc caaagaaatc accgagaatg ctttaggcac gattactagc
     1201 gcctttgatc cagggtctgt agtcaaggct ggtactttga cagcaggctg ggcaagtaac
     1261 gcgatcagcg gaaatcaagt tttgattgat gagccgattc gtctgcaagg tgcttcagaa
     1321 aaaagctctg tctttaatcg aagcggacaa gttgctcttg atgcagtcaa agcatcggaa
     1381 ttgtcttcta atacgtatat gatcaaagtg gcgttaaaga tgttaggact agactataca
     1441 cctggaatgg gactacctag tttagatgaa gaagctaaag cttatcagca attgagagac
     1501 tctttcaaag agtttggtct tggtacaacg actgggatcg atttgccgaa tgaatctcct
     1561 gggatctcac gttctgttga ttatatgaag aagttcaatg cggataatgg caaagaatgg
     1621 tatgctccag gtaactttac cgatttggca tttggacagt ttgacacata tacaccaatc
     1681 caattagctc aatatgcttc aacagtcgct aatggcggga aacgagtaca gccgcaccta
     1741 gtcaaagcga tctatggcaa tgacgaaaat ggaaacttgg gtgaagtgaa gaaagaaatt
     1801 ggcacaacag tagaaaatac ggtcaatatt tctgcagaaa atatggcgat cctccgtgaa
     1861 ggattccacc aagttgtcca tggtaccgat ccttacacaa cagccaaacc attagccagt
     1921 gcgaaaatgg acctttccgc aaaaacaggg actgcagaaa cggttgcgga aggtcatcca
     1981 gatattgcga cagtcaatag taatatcgtg gcatacggac caacagacaa tccggaaatt
     2041 gccatcagtg ttgtcttgcc gaacttatta gacgaacaag accacataaa cttgacgatt
     2101 gctaaagaaa taatggacgc gtattatgat atgttcatgg caaaataaaa aagaggtcgt
     2161 gaaaaagccg cttagtttcg agtatgaaga aaaattttgt gaaaatagct tctcacattt
     2221 ttgcttaaat ttgacttcat accgagaaac tggcttttga acaccgttta ctcggcaaaa
     2281 aaggttgtga caaaa
//
LOCUS       DANKAY010000211         2277 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000211 DANKAY010000000
VERSION     DANKAY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1799
                     /gene="infB"
                     /locus_tag="PWY98_002995"
     CDS             <1..1799
                     /gene="infB"
                     /locus_tag="PWY98_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2038666.1"
                     /translation="TKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLF
                     MLGVMVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPP
                     VVTIMGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAA
                     FTSMRARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQ
                     HVMQELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAI
                     GTVIEARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPAT
                     PVEITGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLK
                     EGELKEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASN
                     AIIIGFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQM
                     IVRETFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVK
                     MGYECGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            1826..2173
                     /gene="rbfA"
                     /locus_tag="PWY98_002996"
     CDS             1826..2173
                     /gene="rbfA"
                     /locus_tag="PWY98_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2038667.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT          769 a          443 c          522 g          543 t
ORIGIN      
        1 caactaaacc agctgtacca ccacgcaaat tccgtgaatt gccagatgta ttagagtata
       61 ctgaaggtat gaacgtagca gatattgcga aaaaaattca tcgtgagcca gcagaaatca
      121 ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata
      181 cgattgaact gttagcgacg gactatggca tggaaccaca agaaaaagtc caagttgaca
      241 ttgctgatat cgataaattc ttcgaggcag aagaagtcga tccagataaa ttagtctcac
      301 gtccgccagt cgtaacgatt atgggacacg tagaccatgg gaaaacaact ttattggata
      361 cattacgcca ttcacgtgta acaagcggcg aagccggcgg aatcacacag catatcggtg
      421 cctaccagat cgatattgat ggtaaaccaa tcactttctt ggatacacca ggacatgcgg
      481 cctttacaag tatgcgtgca cgtggagcaa gtatcacaga tatcacgatc ttagttgttg
      541 ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat caaccatgcc aaagctgcag
      601 gcgttccaat catcgtcgct gtaaataaaa ttgataaacc aggtgcaaac cctcaacatg
      661 ttatgcaaga attaagtgag tatgagttaa ttcctgaagc atggggtgga gaaacgatct
      721 ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga attgctggaa atgatcctgt
      781 tagtcgcaga agtagaagat ttaaaagcag atccaacaca acgcgcaatc ggaacagtga
      841 tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa
      901 cacttcatgt tggtgaccca atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga
      961 caaacgatct tggccgtcgc gataaagaag caggacctgc aacgccagta gaaatcactg
     1021 gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag
     1081 cacgtgcagc gggtgaagaa cgcgcgaaac gtgcattatt agaaaaccgt gctgccacaa
     1141 gccgtgtgac tttggataac ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta
     1201 acgtaatcat caaagcagat gtacaaggtt cagcagaagc actggctgca tcattgaaga
     1261 agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg
     1321 aaagtgatgt aaccttagca gcagccagca acgctattat catcggtttc aacgttcgtc
     1381 ctacaccgca ggcaaaagtg caagctgata ctgaagaagt cgatattcgt cttcaccgta
     1441 tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat
     1501 ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg
     1561 gtacgattgc aggtgcctat gtaacagaag gatatattcg tagagatagc ggtgtccgtg
     1621 taatccgtga cggaatcgta atctatgaag gccaacttgc aagtctgaaa cgattcaaag
     1681 atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc aatgattgaa aaattcaatg
     1741 acatcaaagt agatgacgta attgaaggat ttatcatgga agaagtcaaa aatgactaaa
     1801 aactgataaa aggaggccaa tcattatggc taactaccgt gaccgccgag tcggtcaaga
     1861 aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa
     1921 cgtaacgatc acagatgtga aagttacggg ggacttacag caagcaacga tctattacag
     1981 cattttatct gacttagcat cagataagaa aacagcacaa gatggattga ataaagcatc
     2041 aggattgatt cgtcgagagt tagggcacaa tctaagtatt tataagacac ctgaattgtt
     2101 ctttgaactt gatgaatcag ttgtctatgg caaccatatc gatgaattgc ttcgcgactt
     2161 gaacaaagac taatcaaaaa ctcccgcaaa tcctgtgaaa tcaggctctt tgtcaataag
     2221 gactgatgag ttgtgcaaaa tcaaatctga gtcagaatga accacattct ggctcag
//
LOCUS       DANKAY010000212         2218 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000212 DANKAY010000000
VERSION     DANKAY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(51..1598)
                     /locus_tag="PWY98_002997"
     CDS             complement(51..1598)
                     /locus_tag="PWY98_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2038668.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PWY98_002998"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PWY98_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2038669.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..>2218)
                     /locus_tag="PWY98_002999"
     CDS             complement(2043..>2218)
                     /locus_tag="PWY98_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038670.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          622 a          464 c          397 g          735 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaa
//
LOCUS       DANKAY010000213         2187 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000213 DANKAY010000000
VERSION     DANKAY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..914)
                     /locus_tag="PWY98_003000"
     CDS             complement(<1..914)
                     /locus_tag="PWY98_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2038671.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="PWY98_003001"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="PWY98_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2038672.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="PWY98_003002"
     CDS             2006..2176
                     /locus_tag="PWY98_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038673.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          743 a          384 c          419 g          641 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaa
//
LOCUS       DANKAY010000214         2145 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000214 DANKAY010000000
VERSION     DANKAY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(739..1269)
                     /locus_tag="PWY98_003003"
     CDS             complement(739..1269)
                     /locus_tag="PWY98_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HDL2038674.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1266..1883)
                     /locus_tag="PWY98_003004"
     CDS             complement(1266..1883)
                     /locus_tag="PWY98_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2038675.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          697 a          418 c          232 g          798 t
ORIGIN      
        1 tctggagtta aaaatctaca gtaacatcta taagctttgc tagtaatctt cttgggttct
       61 ctctccattg tcaagcaaca tcaaaacacc tacttactat tcgattcttt tctgccaaaa
      121 acgagtcagc atcactcaca acttatgtgt tgctgactcc gactagtcct ttgttcaata
      181 tcaacttatt aaacagatat aatatttttc tttatttcgt atactccaag acttccatct
      241 ttctttcatc ttatctctga aaaaaacaca gcaaagtgcc cttaataaat atacattaga
      301 atttgcaaat tttttcatct taagatatat cctattcata ttctttacaa tcctaatggg
      361 tgattacaaa tctatttagt tcaagttcta cattataaac acctttcttg atagtatcat
      421 ctgctgcaca agcgatgtcg atccacttat taggggattt acgtgctcaa aagtggctat
      481 tccttttggg aagtccatcc ataataatgg tccatggatt tttttagggt cttgtcgttc
      541 aaatttctca aaaactttat tatacttcca tagttttata attatttggt ttcactttgg
      601 ctcgttcaaa caccaacttg tatttctttt caacgttcga actggagccc cccctataat
      661 ctcattatta ttttcatgac tttttactac cgtggaatta caagcaacaa caatattatc
      721 tgctaaatga acacctttct atatcacgct ttttgctcca atccaacaat gtttacctat
      781 aactatttta ttatttttat tcaaaatcct atgagtattc aattcaaaaa tcgtatgtga
      841 atcggtatcc ataaataatg tatcccacga tactgtacag ccatctttga attgaatttc
      901 cttaaagcaa attatactca tttttcctgt cgaattaaac tttgacccaa tatctaaaac
      961 tgcatttttc cttatgtcaa tatgagcccc ttgtgcgata aacgctggcc cttgtattct
     1021 tagtattcct tcaatactta taatcgtttc ttcatttccc gcagtattta atactggaaa
     1081 gcccaaataa actatttttc cagattgtaa tactatctca attttccctc tcgatttgcg
     1141 tgtaatccgt actttacgat gcaccaaaat aggtaatctc cacgcatctg aaatatgaag
     1201 aaatttataa ttgaagtata tcgttttagg taaatttaat atattcttta ttatcaatcc
     1261 gagtctcatc tttcaccatc tctttttgta atcaaaatta gtttataaag gattacaccc
     1321 aatataccgc ccaacgtatt atatacaaaa tccgaaattt gaaacgttcc tagcctcaaa
     1381 aaaagttgta aaaattcaat tattgctgaa aaataaaatg caatttttcc tccatatact
     1441 attgaggacc atattttggg tttctttata ttttcatatt taatagacat taccatataa
     1501 acatatggaa taaaaagaat aatattttct aaacacgctg tagataactc ttttgttcca
     1561 gttgttgtat tatacttata aatccaccag tctcccataa catcagataa aggatttaac
     1621 caaattgttc gattgattaa tgttgtaaat aaaatcatcg cagtatagaa tatcaaagca
     1681 caaacttttc tatattttct atccttcttt aaatgaagat aaagttccat tagtatacgt
     1741 ttatagccct cacctgcagc aatgccatct gttgcataca taaaatagac cagtcctaaa
     1801 attgtcaata aaacagcaaa cccaaagggt tcatataatt tatataatat atctgtaata
     1861 atttctgcaa tcactattat cataattatt tctccaatat caagtataca cttatcgctt
     1921 ccaattacac atgatcatat tagagtgtat tatcacattc tctaatattc aatttatttt
     1981 tcctaaaata ttcgaaataa tcgtcttgat aacgagtaag tatttttata cgattgataa
     2041 catagtaaca atatttttaa ttatagtaaa aaatcatctg cctttttcat ccacctaatc
     2101 ttatttttca ttttattcaa catccttaaa taaacttccg ttcct
//
LOCUS       DANKAY010000215         2142 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000215 DANKAY010000000
VERSION     DANKAY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            278..787
                     /locus_tag="PWY98_003005"
     CDS             278..787
                     /locus_tag="PWY98_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2038676.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWY98_003006"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWY98_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2038677.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          747 a          374 c          414 g          607 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata at
//
LOCUS       DANKAY010000216         2141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000216 DANKAY010000000
VERSION     DANKAY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWY98_003007"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWY98_003007"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2038678.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PWY98_003008"
     CDS             complement(1297..2133)
                     /locus_tag="PWY98_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038679.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
BASE COUNT          603 a          459 c          344 g          735 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc c
//
LOCUS       DANKAY010000217         2086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000217 DANKAY010000000
VERSION     DANKAY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            50..1000
                     /gene="yidC"
                     /locus_tag="PWY98_003009"
     CDS             50..1000
                     /gene="yidC"
                     /locus_tag="PWY98_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2038680.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1179..1379)
                     /locus_tag="PWY98_003010"
     CDS             complement(1179..1379)
                     /locus_tag="PWY98_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038681.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT          646 a          438 c          356 g          646 t
ORIGIN      
        1 ttttagtata gttaagtttg tgtaaaaatt atagaagagg aagtattcta tgaataaatg
       61 gaaaaactgg ttattgggat ccggacttgt cagtatcctg ctctttttat ctggctgtgt
      121 gcggatggat tcaaacggaa accctgacac ttcgggaatc gtttatcgag tactcgtcca
      181 tcctatgggc caagcgatta catatctagt tgaaaatttc aattggtctt atggctgggc
      241 agttattgca atgactgtga tcgtccgtat catcatcttg ccactaggta ttagtcaatc
      301 gaaaaaaaca atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag ttgaagcagc
      361 ccaacaaaaa ttgaaagctg caacaacacg tgaagaacaa atggcagcac aagcagaaat
      421 gcagcaagta taccgcgaaa acggattgag tatgaccggc ggaatcggct gtctgcctct
      481 attgatccaa atgccgatct tctctgcctt gtactttacg gcacgttata cagaaggtat
      541 ccgggaatca tctttctacg gcatcgattt aggctcacca agtatggtac ttgttgccat
      601 tgcaggggtc gcttatctgc ttcaaggata catctcaacg atcggtattc ctgaagaaca
      661 gaaaaagacg atgcgtacga tgctgatcgt ctctcctgct atgatcgtct tcatgtcgat
      721 cagtgcgcca gccggtgtta cactttactg ggtagtcggt ggtgtgttca gctgtctgca
      781 aacattcatc acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg cagaagagtt
      841 gaaacaaaac ccaccaaaac aagtcgtcac tccaagaatt gatgtgacag aatcagctaa
      901 accagctgac agcaaacaag caaccaaaca aatcaagcaa cccaaaaaca gtacaaacaa
      961 taaaggccgc aacgcaggaa agcagcagaa gcggaagtag atagtaaata agagtgtaat
     1021 aaaaattcat taacagtttt agcaattaaa aatatcgacc aagatttttg aaaaagaaat
     1081 ctggtcgata tttttattta agtgaatgtt taatttgaat catcgattga tcgctatcta
     1141 attgcagcag catcttttta tgaagaaaca actttttctt atctatcaac gtagacaggt
     1201 gttgctgttt cttctaatag tgtacttaat tccttaatat tttgttttcc ttcttctaac
     1261 atccaactac tcgctttttc ttcgccttcg gtaacaaaag gcgccactcc cttcgcccat
     1321 gttgctcgcc cacataagac accattctcc ataaaggaca acgcggagat accacccatc
     1381 taaataacat ccataaacct ttctagtaga agaatagtga tagtatccat ccctttttct
     1441 actgggaata caattacacg attcaaagct tgatacacaa catttccctt cttataaacc
     1501 gctattgtaa ccgaattgtt ttgaatcacc tttttgggat agaaactggc tcctttccgc
     1561 aacttcttaa atgttggttt cttttatact ttctttgtag caactacaaa tttcttcaaa
     1621 tctctaaatg tttgcataac aaccgccttt ctgactttta ctcaggatgg tatttaaaaa
     1681 tcagcaaaat aaggcttctc tccatctttt ttatttccta gctgctttaa agacactctt
     1741 gctgtttttt aatctctatc aagggggctc tgaatctatt ttgatgaact tatcttatta
     1801 atttctcaaa tgagctgtca gacacaactg tattttttca atctcatacc ggacaactat
     1861 gccagcttaa aaaaatttcc tctggcaaaa cttctttatt gaaaaatgac aatgaaaaaa
     1921 gaccattctt tttgttgaat gctcattgtg taaacatatt tcctgcgact gctcttcgaa
     1981 ccattctcag tgcaatccct aatttatcta ttaaatcttc ttttagttcc tcatctttca
     2041 tattcaaaat ttcctttgta tgttacacaa gtttgttctc taaata
//
LOCUS       DANKAY010000218         2058 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000218 DANKAY010000000
VERSION     DANKAY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(592..957)
                     /locus_tag="PWY98_003011"
     CDS             complement(592..957)
                     /locus_tag="PWY98_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038682.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1033..1278)
                     /locus_tag="PWY98_003012"
     CDS             complement(1033..1278)
                     /locus_tag="PWY98_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038683.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1348..1602)
                     /locus_tag="PWY98_003013"
     CDS             complement(1348..1602)
                     /locus_tag="PWY98_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038684.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1766..1978)
                     /locus_tag="PWY98_003014"
     CDS             complement(1766..1978)
                     /locus_tag="PWY98_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038685.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          757 a          352 c          367 g          582 t
ORIGIN      
        1 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
       61 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      121 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      181 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      241 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      301 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      361 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      421 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
      481 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
      541 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
      601 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
      661 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
      721 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
      781 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
      841 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
      901 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
      961 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1021 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1081 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1141 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1201 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1261 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1321 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1381 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1441 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     1501 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     1561 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     1621 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     1681 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     1741 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     1801 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     1861 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     1921 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     1981 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2041 ctaaatcgaa taaaatcg
//
LOCUS       DANKAY010000219         2003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000219 DANKAY010000000
VERSION     DANKAY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..378
                     /locus_tag="PWY98_003015"
     CDS             <1..378
                     /locus_tag="PWY98_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2038686.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PWY98_003016"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PWY98_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2038687.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="PWY98_003017"
     CDS             1859..>2003
                     /locus_tag="PWY98_003017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038688.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DANKAY010000220         2000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000220 DANKAY010000000
VERSION     DANKAY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(31..732)
                     /locus_tag="PWY98_003018"
     CDS             complement(31..732)
                     /locus_tag="PWY98_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2038689.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(748..1188)
                     /locus_tag="PWY98_003019"
     CDS             complement(748..1188)
                     /locus_tag="PWY98_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2038690.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1188..1763)
                     /locus_tag="PWY98_003020"
     CDS             complement(1188..1763)
                     /locus_tag="PWY98_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2038691.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          576 a          420 c          308 g          696 t
ORIGIN      
        1 ttttctttcc cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg
       61 gttatccgta aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat
      121 ctgacggatc accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc
      181 cacttgtatt ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa
      241 cgtttcataa taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg
      301 ttttttgata cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg
      361 ccgttttttt ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta
      421 ctgctgtact tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct
      481 atcttcatag atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc
      541 taaaaatcgc ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga
      601 ataaaagagc ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat
      661 ctcttttgca agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa
      721 aatcactgac atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg
      781 caatgtttca aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact
      841 ttgtgtaatc atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt
      901 caacatagtt gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa
      961 gtcaaggata gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac
     1021 ttcctgcgcc catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga
     1081 gtagaaactt gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc
     1141 gatttgctcc tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc
     1201 ttcaataata actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga
     1261 ttgttggaaa agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa
     1321 gtttctaata attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc
     1381 ttgtcgggcg tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta
     1441 cctagacctc cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt
     1501 tcatcgatcg tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca
     1561 aaaagcccat tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta
     1621 atcactccca taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc
     1681 atgtattttc ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa
     1741 ccgcaaaaaa cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt
     1801 ctacacgtgt ttttttaaaa taaaagacta gattttgtag ttttttagtg gtatatagga
     1861 tagacgccac tatatatagt atttagaaaa aaatcatttt tcagccattt tatagataaa
     1921 acaaaaataa ataacgtgtt ttcatcgata tgaaatgact tacttataga agatactggg
     1981 atttcgtcct atatgccaat
//
LOCUS       DANKAY010000221         1979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000221 DANKAY010000000
VERSION     DANKAY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(325..528)
                     /locus_tag="PWY98_003021"
     CDS             complement(325..528)
                     /locus_tag="PWY98_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038692.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="PWY98_003022"
     CDS             complement(728..1744)
                     /locus_tag="PWY98_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2038693.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DANKAY010000222         1918 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000222 DANKAY010000000
VERSION     DANKAY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..236
                     /locus_tag="PWY98_003023"
     CDS             <1..236
                     /locus_tag="PWY98_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2038694.1"
                     /translation="NGKNEPVCIIKNINVTILPFSEVTKEHAWKEGEGDRTLAYWRKV
                     HAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            345..1028
                     /locus_tag="PWY98_003024"
     CDS             345..1028
                     /locus_tag="PWY98_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2038695.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            1175..1789
                     /locus_tag="PWY98_003025"
     CDS             1175..1789
                     /locus_tag="PWY98_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2038696.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
BASE COUNT          698 a          310 c          404 g          506 t
ORIGIN      
        1 tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat aaatgtgacc atcttgcctt
       61 ttagtgaagt cacgaaagag catgcttgga aagaaggaga aggggatcgg acattagctt
      121 attggcggaa agtccatgct gagtttttta aacatgctta taaagaagcg ttgaacatag
      181 aatttacaga agagaagctg gtcgtatacg aagaatttga agtaattttc ttttagtaag
      241 aaaggtctgc tgaaagaaat agctcattat tgtctcctga atagaaaagg gttacatttt
      301 tatatgaagc tgtattttta tataacatta ctggaggaga tattgtggat acactggaac
      361 aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga aaacgagccg ctacacagtt
      421 cttttattat ggggagaatg gctttggctg caaaagaagg cggagcagtt ggtattcgag
      481 caaatacgac agaagatatc aatgaaatca agcgaatcgt tgatttaccg gtaataggga
      541 ttatcaaaag agattacgat gggtcagaag tgtttattac tgcgacagaa aaagaagtgg
      601 aagaactttt ggagacgcaa gcagaaatca ttgcactgga tgcaacttct cgtaaacgtc
      661 cgacagaaaa aacaactgct gaacttgtcc aaatgattca cgaacacggt cggttagcta
      721 tggcagatat ttcgactttt gaggaagcgc ttcaagcgca agaagatggt tttgatcttt
      781 tgtctacgac tttagcaggc tatacagaat attcacgaaa gacgcagaca cctgattttg
      841 atttattgaa tcaaatcata gaacaagtcg atatcccagt aattatggaa ggacatactg
      901 actctcctga acaagtagag aaagcatatg aattaggtgc tttttcagtc gtagttggat
      961 ctattatcac ccgtccgcaa ttgatcacta aaagatatgt cgaagccgca agtaaagcaa
     1021 ataactaaaa aacacgcacg gcacagattc ttgacaaaaa agaaaaattg atagatagtt
     1081 taaagcagat tctaagaaga tgaaaaaagc attacttttc attttgtctt caagatctgc
     1141 tttttaacga attttataga aagaaggcga aaccatgttt gaacggagtc cttcagaatt
     1201 gacagaacgt gaaaattaca agttgctgat tggttcagtc atccctagac cagtagcagt
     1261 agtaacgact aaatcagcaa aagatatagt gaatatcgca cccttcagtt acttcaatat
     1321 cgtcagttcg aatcgaccca tcttatcctt ggcgatccag agaaaacaag atgcagtaaa
     1381 agacacagca aagaatattc ttgagacaag agaagctgtt atccatatcc ttgatgagga
     1441 caacgtagaa gaagtaaata aaacagcagc caatctgcct aatgaggaaa gcgaactgta
     1501 cctgactgac ttgactttaa ctgattcagt aatcatttca gtaccagcat tgacagaagc
     1561 aaaagttcgt tttgaaacat ccttatatga acatgtcgag atacggaata aaaataaagt
     1621 aacagctgat ttatttttgc tagaagtaca gaaatatcat ttaaatgaaa cagtctatga
     1681 tgataagaca ggaaggatcg atccaagaaa attgaatgct gtaagccgtt tggcaggaaa
     1741 tgattatgca gcaataggag aaattttcac gattgcacgt cccgaataaa aaggagcact
     1801 agttatgtta aaaataagct ataaaccatc gacagattca aaagaaatga aaaaagaata
     1861 tgaaacagtg aatgattttc tgcaagggca gtacttagaa gttccgccat tgcaagac
//
LOCUS       DANKAY010000223         1916 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000223 DANKAY010000000
VERSION     DANKAY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            337..693
                     /locus_tag="PWY98_003026"
     CDS             337..693
                     /locus_tag="PWY98_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2038697.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            703..1602
                     /locus_tag="PWY98_003027"
     CDS             703..1602
                     /locus_tag="PWY98_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2038698.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          651 a          312 c          381 g          572 t
ORIGIN      
        1 gtcttttatt tgataagttt actctaccga agagttgtta ataatccgtg aacaaaaagt
       61 aattcttttt ccaatagttg ttaaaggttt tttatttttt atagtatttt ttgttattta
      121 agaaaggaat aaggttaaac aaataacagg ttgctttttg ggtctttttc cgttcaagaa
      181 actgaacgaa gaaaagggaa agttgtctct tttgtgataa gagaaatcat aataaacgaa
      241 tgacttactt tccaataaat tgagaacagt cagaaccaga caaattagtt gcatctgatg
      301 aataaagtga ttattctatg acatgaggtg atacagatga aattagaagt gacaaaagaa
      361 gcacaagaag tattgaagaa taaattagaa gcagacgatc aacttttatt agatatcgaa
      421 aacggtgacg gtccatttgc agaaagccaa gtcagttgcc aattggatac ggcatttcga
      481 ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc tttattctgt agtggctgat
      541 acaccagttg gaccaatcaa aatcaaagac agtgcaatac tttacatgga tgatccatcc
      601 acacttgctt tagaaccaac ttataatagt ttacaattaa aaggaccaag cggcttgaga
      661 aaaggcaact tgcaagttgt tcgaaaagac tagagggata aaatgggaaa attggcgatc
      721 atcagcgacc tacatgtgga catcaataaa ttcggcgaat cagagctgat gcagctttgg
      781 caagttttgc aaaagcacaa gatcactcgg cttcatctag ccggagatac ggctaataaa
      841 gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata aaggattatc tacgacattt
      901 catttcggca atcacgagtt ggcagatgtg cccaatgaac agatgatgga ccattttcca
      961 gacccacatt ttttaaatga acggtatatt gctttaaatg agcagaccgt tcttttaggg
     1021 atgaatggct ggtacgacta tcagttttcc gaattgaaag attacgaggc aatcgtacga
     1081 atgaagcgtt tgtattggta tgatcggttc atccagcgtg ggaaaacaga tctcgaggtc
     1141 aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc agctggaagc aaaaaaactt
     1201 gaggtgattt tatccactca tttcgttcct aaacgggcat ttatcatgta tcaatctgct
     1261 ccttatgagc gttggaacaa attaaatgca tttttaggat cagaatcatt cggagaatta
     1321 cttgaccgtt attcaaatat caaacaagta gtttttggcc atacgcatcg tcgctttgaa
     1381 gataaatcca tcggtcacac gatttacagc tgcagacctt ttggttatta ttatgaatgg
     1441 cagctgaccc gagattttgt cttgaaagaa cagttgctag acagctatga tcctatgaaa
     1501 ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg cctatcaaac acaacatttg
     1561 caagaggaat ttgaacaagc gatgacaatc gttccgtatt gagacagcat tagtaatgtg
     1621 taaaagaaaa taccttgtaa aacataagga taagtaagag cagtagatcc atttatcgtg
     1681 gatatgctgc tttttttgcg gaaaataatc agcgaatgtt tatttgatcg gatgatttgt
     1741 aactcgcaga gtactagatg caagcgtgta ttcttttttt gaacgataat cacaaaaaaa
     1801 aatataatat acatttttta tttaattata attaatattg attgaagtct aggcagtcaa
     1861 gtcgagagtg taaaatattt tgtgtaaatg aaaaaatcca tacaaaaaag gaagtc
//
LOCUS       DANKAY010000224         1888 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000224 DANKAY010000000
VERSION     DANKAY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..435
                     /locus_tag="PWY98_003028"
     CDS             <1..435
                     /locus_tag="PWY98_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2038699.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="PWY98_003029"
     tRNA            complement(759..842)
                     /locus_tag="PWY98_003029"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="PWY98_003030"
     CDS             1370..1870
                     /locus_tag="PWY98_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2038700.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          610 a          359 c          321 g          598 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaa
//
LOCUS       DANKAY010000225         1883 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000225 DANKAY010000000
VERSION     DANKAY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(257..1276)
                     /locus_tag="PWY98_003031"
     CDS             complement(257..1276)
                     /locus_tag="PWY98_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2038701.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1273..1497)
                     /locus_tag="PWY98_003032"
     CDS             complement(1273..1497)
                     /locus_tag="PWY98_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2038702.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1494..1787)
                     /locus_tag="PWY98_003033"
     CDS             complement(1494..1787)
                     /locus_tag="PWY98_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038703.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          529 a          390 c          329 g          635 t
ORIGIN      
        1 ctgtaggact cgaacctacg accggacggt tatgagccgt ctgctctgac caactgagct
       61 aagggtactg gttgttgcca cataaagcca taaacaatca accagtagaa tgtgtggcaa
      121 caaacctgtt atcgcatagc ttggagtgtg actattatgg gtgatagtga agatatgcga
      181 taacatcact attttatcga atgattttta tagttgtcaa tatagttatg taatgctcct
      241 caacgaggag ctatttttat cgtttaggaa tatttaaata ccaacgtttg tcatggaaat
      301 cttgcgcacc gcctttagtg tttccctctg gatcattcgt tgcccgcatc atgacataga
      361 ctttcttatt agggaagtta cgcatgttaa aagatacatg atagccaaca tttccagaag
      421 tattataagc ttgatttaca tctgatctat aaattccatc agctcttact cgagctaatt
      481 ctttcccagt attgtaatcc ataatgaaga tatactcgta tttatagtta gcaatgtgcc
      541 atccagctac atgcaaattt gcattttcga tttctccaaa ctgatcaatg tgggcgtaat
      601 tcgttccatc tgtcagtgta ggattagctg caccagctcg tgttggatca atgacaggct
      661 tgttttcaga agttgttgga ttttcatcgg taaatccatg agctaaatca tatgctaatt
      721 tttctttact tacgcccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaga
      781 tgttttgtgt tacccataaa tgcgatttga ttcccggttg gttataagga gtgtccaacg
      841 ttaatggaat accatatttc attgctgaat ctctagccaa ttcaacgtat gccttgtagt
      901 ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc aatctgcaca ggactgttgg
      961 cattagcata cgaaccagca ccgtactgta cataaccagg ttgaccgact tgataaacaa
     1021 ttccgccgtc tcccacaata taagcagtat aagcgctagt ccatgaacgt tgcatatact
     1081 gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc atgcagtaaa atatactgat
     1141 tatttgctac ttgtgagcta ccttcatttg cgcccaaatt aaattcattg ttgatagtat
     1201 aggcaaatcc gttaattggc aataaaaaaa gagccgttaa caggctcatc gcagtaatag
     1261 taattttctt tttcatttgt ttcctccttc ttcgctttca gccgagaaca ttttgtaggt
     1321 tcgatttgat acacccaaca cactccctaa aaacgcgcca aaaccagtaa tgatgacaac
     1381 acagatatct gtgtactgcc aattgagcgc tttaccaact aaccccacga aagtagctag
     1441 tgcgggaata attaccagtg cgaaccattt tagtacttcg aacgttttat tattcatttt
     1501 cttctctccc taaacaaagt tttgatttgt tgcgtgtgtt ccaccaattt ttctgtatgt
     1561 gtatctaatc tttcatcgtg tttctttagt tcttcatgaa tcatcaatcg atctgatttg
     1621 ctcgattcta aatctttagt cagcaaatct aaattgtggc ttacttttga aagagtctca
     1681 gtaattttcg agaaagatgc agcaatcggt ctaattacta ataaaatcaa agaaacgata
     1741 gcggttattg atcctgctat cattccccat tcccctaaat taatcatgtg acaactcctt
     1801 taccttaaat aaaaacgcat caattaagat gcgctcttat ctttattaat gattttatct
     1861 gcttcttcgt ctgtaatgca taa
//
LOCUS       DANKAY010000226         1840 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000226 DANKAY010000000
VERSION     DANKAY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            98..391
                     /locus_tag="PWY98_003034"
     CDS             98..391
                     /locus_tag="PWY98_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038704.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            388..612
                     /locus_tag="PWY98_003035"
     CDS             388..612
                     /locus_tag="PWY98_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2038705.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            609..1634
                     /locus_tag="PWY98_003036"
     CDS             609..1634
                     /locus_tag="PWY98_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2038706.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT          627 a          305 c          375 g          533 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
      421 ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc
      481 aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg
      541 agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga
      601 ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt
      661 tattacccat taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc
      721 aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg
      781 caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc aattcttata
      841 ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt
      901 cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat
      961 atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg
     1021 caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa
     1081 tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt
     1141 acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct catggattta
     1201 ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg
     1261 cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag tttggggaaa
     1321 tcgaaaacgc aaacttgcat gtggctggat ggcacattgc taactataaa tacgagtata
     1381 ttttcattat ggactacaat actgggaaag aattagctcg agtaagagct gatggaattt
     1441 atagaccaga tgtaaaccaa gcttataata cttctggaaa tgttggttat catgtatctt
     1501 tcaatatgcg taattttcct agtaagaaag tctatgtaat gatgcgggca acgaatgatc
     1561 cagagggaaa cactaaaggc ggtgcgcaag atttccatga caaacgttgg tatttaaata
     1621 ttcctaaacg ataaaaatag ctcctcgttg aggagcagta cataactata ttgacaacta
     1681 taaaaattat tcgataaaat agtgatgtta tcgcatatct tcactatcac ccataaatag
     1741 tcacactcca agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt
     1801 atggctttat gtggcaacaa ccagtaccct tagctcagtt
//
LOCUS       DANKAY010000227         1832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000227 DANKAY010000000
VERSION     DANKAY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            141..1607
                     /locus_tag="PWY98_003037"
     CDS             141..1607
                     /locus_tag="PWY98_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2038707.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          572 a          315 c          367 g          578 t
ORIGIN      
        1 ttttgatgga aaaaatatga aaaaacgatg tgtattaaat aaaagaatac acacataaaa
       61 taacactgtc ttgtaagttt gttaaaaatt gaatatgatt acatctggat aacaaatttt
      121 tatcataaag gagagaacga atgaacaaca aacaattacg ctggtttact gttgcgttaa
      181 ttgcatttaa catggtttgg ggcatgggga atgtagttaa taactatgct cagcaaggag
      241 tatcagttgt cacctcttgg ctattgattt tggccattta ttttattcca tacgcattga
      301 tcgttggaca attaggatca gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg
      361 aaaatacgac atctaataaa cgactggcat actatgccgc ttggacttac tgggtcgtcc
      421 atattcctta tttggcacaa aagcctcaag caattctgat tgctggtggt tgggcagtcc
      481 aaggaaatgg aaatatcgta aatacgatgt cagtacaaat ggtagcagtt atttcactta
      541 ttatatttct tgccttttta tatttatcaa caaaaggact ttctactttg aaagtgattg
      601 gcggactggc cggaacagcg atgttcgtca tgtcgatctt atttattctt ttagctgtaa
      661 cggcaccgtt catcaatcca acgatggaat ttgccacacc acatatgaat caagtgaaaa
      721 catatattcc aacatttgat ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag
      781 ttggaggggc ggaaaaaata tccccttatg tcaactctac aagaaatcct gcaaaggaat
      841 ttcctaaagg gatgatcttc ttagcagcaa tggtagggat ttgtgctgta ttaggctcag
      901 tcgcaatggg tatgctgttt gctagcgata atatccctaa agacttgatg gcaaatgggg
      961 catacagtgc ttttcaaatt ttaggtaatt actatggtat cggtaactca ttgatgatca
     1021 tttacgcatt gacaaacatg attggacaaa tctccgcatt agccttttca attgatgcac
     1081 cactaaaaat cttgttagca gatgcggatc cagaatttgt accaaactgg ctacgtaaac
     1141 gtaatcgtaa aggtacattg gtgaatggtt atcttctgac aggtatacta gtaggaatca
     1201 ttattttgtt gccgatcttt ggtatcaaag atatgaatga attagtaaaa tggttgacga
     1261 acttgaactc agttgtcatg ccaatgcgtt acctatgggt gttcttggca tatatgatgt
     1321 tgaataaagc ttataagaaa ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg
     1381 gttttatctt tggtgcttgg tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg
     1441 tgccaaaagt agattatact gctgatccta aagcatggtg gttccaatta ttcacgaata
     1501 tcatcacacc aattgtcttg atcttattag gtatgatctt gccagcaatt gctcgtcgag
     1561 acaaaaacaa agaaattact gaaccagaaa cagtggttca gccttaaaat agaaatactt
     1621 gttacaagag gttgtgacag aagctacttc tggaacaccg tgaatcagag gttgtgaaaa
     1681 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagctcctca tattttgccg
     1741 ttttttgagc taatgctgaa ggtcagctga ttgaacacca tttattcgaa acaagggact
     1801 ttgacacgac ttttgtcaca gtcccttgtt tt
//
LOCUS       DANKAY010000228         1761 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000228 DANKAY010000000
VERSION     DANKAY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            275..1753
                     /locus_tag="PWY98_003038"
     CDS             275..1753
                     /locus_tag="PWY98_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038708.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          645 a          246 c          314 g          556 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat g
//
LOCUS       DANKAY010000229         1754 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000229 DANKAY010000000
VERSION     DANKAY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            162..758
                     /locus_tag="PWY98_003039"
     CDS             162..758
                     /locus_tag="PWY98_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2038709.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(816..1298)
                     /locus_tag="PWY98_003040"
     CDS             complement(816..1298)
                     /locus_tag="PWY98_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038710.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1404..1601)
                     /locus_tag="PWY98_003041"
     CDS             complement(1404..1601)
                     /locus_tag="PWY98_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038711.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          584 a          355 c          269 g          546 t
ORIGIN      
        1 aagaaaatcc ttacataacg atttttctta ttaaaattgt tttttgaact tcgcttattt
       61 tttgtgagca tatctgttta cttacttttt gtaaggtgct atacttcatt tcgtaatcaa
      121 ttaaaaaaat gcaaaacgca aatcaaagga gaaattttat tatgacaact tttactgaca
      181 ctttgaaaaa ccgccgttca atctatcatc taggacgtaa tgtttcactt tctaatgaag
      241 aattaacaac tttgatcaaa gaagcaatta aagaaagccc aactgctttc aatgctcaat
      301 caacacgcgc agttatcctt ttcggagatg cacatgaaaa actttgggaa atcactgaag
      361 aagcacttcg cccattgact ccagcagaag ctttcccaaa tacacaaaac aaattagctg
      421 gtttcaaaaa tggttatggt acagtattat tcttcaaaga tactgacgtg gtaaaaggtc
      481 tacaagaaca atttgaatta tatgcagaca acttcccaga ttggtcagaa caatcaaacg
      541 gaatcgcaac agctaatact tgggtagcat tagttgataa aggattgggc gcaaacttgc
      601 aacattacaa cccagttatt gacgaagcag ttgcaaaaga atggaatatc ccttcaaact
      661 ggaaactacg ttcgcaatta gtattcggtt caccagaaac accggctgag gaaaaagaat
      721 acatgaacga tgccgatcgc ttccgcgtat ttggataatt ttatttgaaa taaaaaacag
      781 aggttgtgac aaacatcttg tcgcaacctc tgtttttatt ctggatcaat cataatatca
      841 atctctgcct catcgtttaa gcgttgggac aaaaatggaa ctaattcttc taatgatgca
      901 gaaaatttca gcatcggtcc tcgccatccg tttcggaaaa aattcatttt tacgatttta
      961 gaaccttttg gctgatataa tacaatttta ttgatctcag aaaaaggaac accttttttg
     1021 tcaaagctat tcaaaacaag tccttcttct gtgattcctc tactgtccaa caaaaaacta
     1081 ataaaaatca ttgctgacaa gatccctttt gccaattcat ctgctgtagg atgactgatg
     1141 agtgtatacc aaatgactag tgacactaag aagggtatca ccaagtagac ccatcgattt
     1201 cgcacagtcc gataaataat ttcttttcga accagccaaa gaaataaaaa catcacaaca
     1261 acgaataaaa acaatagaac cgtaaagaat gttaccatga caactcgcct cgctttttct
     1321 tctccagaaa ataggaaaag aatactgatt taccttctca aagaaattgg acttgcagca
     1381 cgccgcacgt ccaattgttt tctttattca aatgtatcat attttgcaac aaccatgctt
     1441 gctttttcat ctaataattc ttggtcctct gcagcacatt gggcaacttt gaccacggcg
     1501 cagtttgtca tttttgatac aacttcacca atgaccgttt ttgtaaaacc aattggtttg
     1561 catgcatatt gttctcctaa attaatcatt tcttccgtca tcaaaatcct cctaaactgc
     1621 tggaactttt ctccacatta ccactgctat ttctttggag atttaactca ttgattttta
     1681 tttacagtta aaaatcaacc ttacaggcat tatagcgtac tgttttcgtt ttcacaatta
     1741 aaacagctgt tgta
//
LOCUS       DANKAY010000230         1721 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000230 DANKAY010000000
VERSION     DANKAY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..146
                     /locus_tag="PWY98_003042"
     CDS             <1..146
                     /locus_tag="PWY98_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2038712.1"
                     /translation="HTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVI
                     PPK"
     gene            159..1067
                     /locus_tag="PWY98_003043"
     CDS             159..1067
                     /locus_tag="PWY98_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2038713.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1067..1687
                     /locus_tag="PWY98_003044"
     CDS             1067..1687
                     /locus_tag="PWY98_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038714.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          610 a          250 c          392 g          469 t
ORIGIN      
        1 gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat gaacaaaaca
       61 cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca agtgcaattt
      121 cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg aatgtcatat
      181 taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa ggcaataaga
      241 atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat ttatcagaat
      301 gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta gaaacaaaaa
      361 ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc atgcagtgtt
      421 tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt ggaacaactc
      481 aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa atgggttctt
      541 attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat gaaaataaag
      601 gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg agacaagaac
      661 aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg gcatggtttg
      721 aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta gccgaactaa
      781 cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag cgtttgctgg
      841 cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt gaatacgggg
      901 agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat gaagtcgaaa
      961 aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca accgttgatg
     1021 atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg ctaaaactaa
     1081 acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc caatcacaca
     1141 tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag ttttatctgt
     1201 taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag agaatgttct
     1261 cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag aaatgtatca
     1321 aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta tcgaccgttt
     1381 gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag tctctgcagt
     1441 cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt cactgaaaaa
     1501 ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt ctacaggtgg
     1561 tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag aagctgtaaa
     1621 tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa aaattgggga
     1681 ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DANKAY010000231         1699 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000231 DANKAY010000000
VERSION     DANKAY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            84..1292
                     /locus_tag="PWY98_003045"
     CDS             84..1292
                     /locus_tag="PWY98_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038715.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            1276..1446
                     /locus_tag="PWY98_003046"
     CDS             1276..1446
                     /locus_tag="PWY98_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038716.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT          645 a          210 c          277 g          567 t
ORIGIN      
        1 aattgaaaat taacgttaaa tcaaaaaaat atttacaaga tgtttattta agtagataat
       61 aaaagaaaaa agcaggtgaa attgtgacag cagaaattgg tattatgaat aaaagtggaa
      121 tcgtgttagc ttcagattca gcatctacga ttggagatag caaagtatat aatactgcaa
      181 aaaaattatt tactttggat tccatgcatt ctgtaggtat catgatatat ggtaatgctg
      241 aatttaatgg tattccttgg gaaataatca ttactcaata taagaaaagc attggtagtt
      301 ctgtttttaa tactttggaa gaatacgcgg ataattttat agaatttgtc aaaacagcat
      361 cttttattag aagtgaacag accgagcaag aacaaatgat tggggttttt caaaaaataa
      421 tttcaggttt atttgagagt atagaagtag atattaactt tttaataagc caaggtacac
      481 aaatagacaa agatgtccta gtgaagttat tacaaacaaa aatgaatacc aatttgtctc
      541 aacagtccca gacattcatt ttagatattg aaaaggcgtt atttcttagt aactatggag
      601 aaatattaaa agatattctt aatagtattt ctacaatgga aggtgtttca gaagctatat
      661 cagaggaaat tcaaagttac gtttatgaaa ttataattag agacgatgtt tattcttccc
      721 ctacaggaat agttatagca ggatatggaa gaatggatat tttccctaaa ctatattctt
      781 ataatatgtt tggatttgtt atgaatattc tgaaatactc agaatatgaa agtgctcaaa
      841 taggaaatga caatggttca ttaagatcta ctatattacc atttgctcag tctgatgtgg
      901 taaatactgt agttcaaggg gtagatccac aaattactaa ttatctgtcg agtcaagtcg
      961 atagttttga cgataacgga aaaaatacct atattaatat tataaagaat atttcagagt
     1021 ttcaacaaaa tcaattcata tttccgttac taaacatgat tgctttactc ccagttgaag
     1081 aaacagcaat tatcgcagag actttattaa atttaaccag ttttaaacga aaatatacta
     1141 cttctgtaga aacagtaggt ggcccaattg atgttttagc tattacacct aatgatggcc
     1201 ctatttggat taaaagaaaa cattattttg atattgataa taatattgga tacagattgc
     1261 gaaaggagag agcgaatgat tacaataatt aaacctcagg aaaaatcccc tgttttgaaa
     1321 tttaatgaga gcaatctagc ccataataaa atgagtataa ctgatacagt atttgatgta
     1381 atggttgagc aacaaaaaaa aattaataat aacaatagac aaagaaaaga aattaaccaa
     1441 tattaatgtt ttttctgttg cgttttttgc agtcataatt taaaacctcg ctcaatcttg
     1501 ggcgaggtct ttttttattt ttctgaaaag ttaaagtaaa taatagcata aaataatata
     1561 ttttacaaag aataagtaca atctagtttt ttgctattaa atgtgtaata ataaatatgc
     1621 catcacaaca tgaagaatga aatccattat tatctagtct agtccattct ttttgtttgc
     1681 agtatttgtg atggctttc
//
LOCUS       DANKAY010000232         1646 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000232 DANKAY010000000
VERSION     DANKAY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..443
                     /locus_tag="PWY98_003047"
     CDS             <1..443
                     /locus_tag="PWY98_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDL2038717.1"
                     /translation="YEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYP
                     IEKQLGMIEEDPNTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAY
                     AKDPVVTRYIRKLFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(465..1619)
                     /locus_tag="PWY98_003048"
     CDS             complement(465..1619)
                     /locus_tag="PWY98_003048"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2038718.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          497 a          329 c          308 g          512 t
ORIGIN      
        1 catatgaaat ggtttttaca gcagcaaatc cagcattggt acatcttatt caattagaaa
       61 aattgatgaa aaagtcaagc aaatggtgcg tgataatcag agtagtggaa tcaatcgatg
      121 atgtttttta tcctatcgaa aagcaactag gaatgataga agaagatcct aatacatcgc
      181 cagttgtaat ggatcaattt gaaacatact taattgatga aggattcaaa gtgaagaaaa
      241 aagattttac ttattttaca caggaatcaa ttactgaaga attgatgaag ctatactatg
      301 acgcgtacgc aaaagatcct gttgtgacca ggtacattcg caaactattc agtgaaaaac
      361 gagaaatcct tagtacgact aagctgacct atagactgct cctttggtcc gcagataaaa
      421 aaagaacctt tcacgactcg tgaagggttc tttttatgat tttattatat tttgtagtaa
      481 cgaatatcat tttcgatctt tgtaatcgaa aaaggtacat ctgaagtata ttctgcacat
      541 tctccgtgtt caggaagcgt ctcactgaaa atcgtttcat attcgtcaac agtgatccgt
      601 gttcgagaat ctagcatctc ttgatgagat tgtgcgaaga gatattcttg atagttttct
      661 tccagataac ctgtaaaaaa ttcacttact gcaccagaac cgtagctgaa tagaccgatg
      721 cgatctcctg gctgtagtga tttcgaattt tccagtagag aagttagccc caggtataat
      781 gaaccagtgt aaagattacc gattcgtcgg ctgtaacgga tgctttcttc atagcgagcc
      841 ataagacgtt cctgattttc ttcgtctgtt tggtctaata cgctttgcaa tgcctttttt
      901 cccatcttag tatacggaat atggaaagca atcgcctgat aatcttcgag tcctcgaccc
      961 gacaattctt tatgtcgatt ccaaactttt tggaatgatt cgatatacgt agaattagat
     1021 aaaggaccat caacaacagg aaattcgcta taatctggac gccagaaatc atagatatct
     1081 tctgtcagaa atacgctgtc gtcttcaatc gataaaatac gcgggttttg agtgatcatc
     1141 atcgcaacag caccgacacc ttgcgtcact tcaccaccgc ttgccaagct gtaacgagca
     1201 atatcacttg ctatgactag tacttttcgt tctggatgat ttttgacata ttctttcgcc
     1261 atgtgcaaag ctgcggtgcc accgtagcaa gcctctttta cttcaaaaga acgagcaaaa
     1321 gattggattt tcagtaaatg gtggataatt accgcactcg cttttgaatg atcaatcccg
     1381 gattctgttc cgacgatgac catgtcgatt agctcacggt ctttttctgt tacgattttg
     1441 ctggcagcat ttgctcctag tgtaatgatg tcttcatttg cacgattgac tgccatttgg
     1501 tcttgaccaa tccctatatg gtattttgca ggatcatccc cacggctttc tgccaactct
     1561 gtcatatcta aatataaatt aggaataaaa aaggaaagac gatcaatccc tattttcatt
     1621 tcaacacctc tttacttatg ataaaa
//
LOCUS       DANKAY010000233         1644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000233 DANKAY010000000
VERSION     DANKAY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..182)
                     /locus_tag="PWY98_003049"
     CDS             complement(<1..182)
                     /locus_tag="PWY98_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2038719.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="PWY98_003050"
     CDS             517..951
                     /locus_tag="PWY98_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2038720.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="PWY98_003051"
     CDS             974..1294
                     /locus_tag="PWY98_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038721.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="PWY98_003052"
     CDS             1435..1626
                     /locus_tag="PWY98_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038722.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          627 a          247 c          350 g          420 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatc
//
LOCUS       DANKAY010000234         1640 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000234 DANKAY010000000
VERSION     DANKAY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(186..1001)
                     /gene="thiD"
                     /locus_tag="PWY98_003053"
     CDS             complement(186..1001)
                     /gene="thiD"
                     /locus_tag="PWY98_003053"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2038723.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1295..1570
                     /locus_tag="PWY98_003054"
     CDS             1295..1570
                     /locus_tag="PWY98_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038724.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          510 a          322 c          251 g          557 t
ORIGIN      
        1 tgttttttga acttgttcct tgtgactcgg agctggacgg caggctcacg acctctttct
       61 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag
      121 aagctgtttt tacttggctg ttcttattac ttgatgcagg gcaacttttt cacaacttct
      181 ttcttttact ctaattcacc agctgcccaa tgattgatgg ggccatattt gtgccctaca
      241 tttatttctt cacgaattgc acggtttaca tagtcttttg ccaatcggat cgcttgttca
      301 actgtttttc ctttcgctaa ctcagctgca atacatgcag atagcgaatc gccagttccg
      361 ttgatccggt cggtaggata aaatggtgca tttaaccaaa atgattttcc tgattcgaac
      421 aatacgtagt cattgactgt ttcttgctgc gcatttttag catgatgtcc tttgatcata
      481 acgttttttg cacccatatc ttgcagttct tttgctgctt gcacaaaatc ttcttcagct
      541 gctaatgtcc gtccagtcag cttttctgct tcataaaaat taggtgtcag tacttcggcc
      601 aaagggacaa gcttttcttt caagctttgc aaagcagatt cttctagtaa aagatttcca
      661 tgtttcgtta cgattacggg atccacgact aatggtccaa aatcatattt ttggtaattt
      721 tttacaactg tctcaatcaa ctttgaatct gctagcatac ctgttttagc tgcacgtatt
      781 tgaaagtcct tcgcaatcag ttcaaattct ttgtcgataa aatctgttgg caatgtcaca
      841 cttgctccga ttccgtaaga attacctgca acacatgcgg tcatgacaga aacaccgtaa
      901 acatttcttg taaagaatgt atgcatatct gcttgcatcc ctgcgcttcc atcgctatct
      961 gatcctgcga tcgtcattgc ttgtacatac tgatcttcca tcaaatcgat tcctccattt
     1021 ttataaaaag atttttcata atactgttac tgtttctatc atatgttttt caaagtatcc
     1081 ctcagtaaat ataccactac cttttcttgg taatcaaatt tatttgaaaa aacatttgca
     1141 ataaatcaga aaatagtggt cgtttatttg tattcgctat tctaataata aataaccact
     1201 atttgattta tatatatttc agtatccttc ttgttttcat ataaccaaag gcgctatgat
     1261 ttccatatga aaaaaataaa gtcaggtgaa aaaaatgaaa cttgaggata aaaaagtcat
     1321 tttgaagaaa atcaaacgac taccagacca aatagatggc tatacgatct gttctttcaa
     1381 aaaagacagg agtataaaag cttatacgca aaacacagaa aaaggaataa tctatttcct
     1441 acatgaagaa ggttacgata cccaaacctt tatgtttact gaaaaaaaag aatttcataa
     1501 acagatgaaa aaactgattg aaaaagaatt tccaagaagt cataaactct atgtgaatca
     1561 gcaattttaa cttctaactg ttctttatta attaaaaatg ttctgtaata aaataaaact
     1621 ttataacaac tataacaaaa
//
LOCUS       DANKAY010000235         1605 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000235 DANKAY010000000
VERSION     DANKAY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            122..754
                     /locus_tag="PWY98_003055"
     CDS             122..754
                     /locus_tag="PWY98_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2038725.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            877..1515
                     /locus_tag="PWY98_003056"
     CDS             877..1515
                     /locus_tag="PWY98_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2038726.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          543 a          243 c          253 g          566 t
ORIGIN      
        1 cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc gaaaacctat ctcattttct
       61 gctaatgtaa gttggtttag cttttgttaa tatgacttta tgaaaatgga ggtaattaaa
      121 tatgattttt cctaaagtta atgaagcaag cttgaataaa ggatcctttt ttcatatcta
      181 tgaaaacgaa gattttgaac tgtataatat cctttcacaa aaagaggtca tcgaaaacca
      241 aataatctgc catccaatta tttacaaatc tattttcaag tatgcatttt ttgcttccaa
      301 tgattttcaa agaatggaga ttaccgactg tatattcgaa aactgtgatt tttcaggctg
      361 tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc aattgtaaat taacaggtac
      421 taaattgatg gaaagttata ttgggaacac gttattcgag aattgtaaaa tggactatgt
      481 aaatttatct ggttcaaaca taaaagaaag tacctttgag cactctgtct tatcaagcgc
      541 agattttgta gactgttccc tcactaaaat aatgttttct actaatgatt tagaaaatgc
      601 tagctttttt gatactgatt taaaaaatat cgatttaagt agcaactcct ttgaaatgat
      661 tgaagtacgc tcggaacatc taaaaaattg tacgcttaat cagttgcaag cacttggttt
      721 tgctagaaaa tttttacaaa taaaagtagt ataatcaaaa tgaatatata agataaattt
      781 ttagcaatac aaatatccat aaaaaaacat tgcctatttt aactcttcat gtatactgac
      841 tctatcttca aaaactgaat agtaggagtt attatcatga ataacatttt aggaaaaaaa
      901 atagaactta aaaaagggat attgggcact attgtcgttg tactcggttt gttagggttg
      961 ctatttgcgc catccattat tagcattttt tctttaacta tcactggatt tatagcattt
     1021 acacttgttt ttggtaaaaa agatacgaaa aaaatgttct cgaaacctgt cgctcctgtc
     1081 aaaaatatag ccaaatattt ttttatcaat gtagtaatca gtgccgcagt ctcggtattt
     1141 ttgcaacaaa ttttgaaatg gggtttaaca ggcaatccaa taaatgaggc tttcagcccg
     1201 ttattattta ttattttgcc tatcatgata cttggagaag aattgttctc agtgtatttt
     1261 ctcgctattt tttctagtaa gttcagcatt cctgtcgcta gtttcttgtc agcaatcatc
     1321 tttggattta tccattacag tacttatgat aatggaaata ttttacacac tgtagcacac
     1381 atccttttga tacaaggggt ggcaagattg ttattcaacc aagctgccat taaaagcaac
     1441 tcgatcatta caagttgggc agttcatgtg atatttgatt tcacagctat attgctagtc
     1501 gttttattct cctaaatttt tatagaaata atacatgctc tattgctaag aaagcatact
     1561 ttatttcctt taaacaaaaa gacagatgta tcaatggatt aaaaa
//
LOCUS       DANKAY010000236         1594 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000236 DANKAY010000000
VERSION     DANKAY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            288..695
                     /locus_tag="PWY98_003057"
     CDS             288..695
                     /locus_tag="PWY98_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038727.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="PWY98_003058"
     CDS             709..1110
                     /locus_tag="PWY98_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038728.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="PWY98_003059"
     CDS             1112..1483
                     /locus_tag="PWY98_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038729.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          520 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtcc
//
LOCUS       DANKAY010000237         1574 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000237 DANKAY010000000
VERSION     DANKAY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            326..1456
                     /locus_tag="PWY98_003060"
     CDS             326..1456
                     /locus_tag="PWY98_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2038730.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          446 a          257 c          341 g          530 t
ORIGIN      
        1 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
       61 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag
      121 ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg
      181 tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat
      241 ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg
      301 gtgaaaaaag atcgattggt ggattttggg tccttatttg actttgtcca tgatcggtct
      361 tttagaagta tacagtgcca gttcttatcg tttattgcaa gccgatgaaa atacaaaaag
      421 tctattattg cgtcaactaa ttttcatatt tttgagttgg agcgtgatct tcttagctcg
      481 ttcagtcaaa ctacactatt tacttcaccc taaaatagca ggatacggtt tagccttatc
      541 gattttcttt ttagtattag taagaatagg gatattcggt gtcactgtca acggcgcaca
      601 acgttggatc tctctgtttg gcattcaatt ccagccttct gaactggcaa atcttttttt
      661 gattttttat ttaagctggt tttttcgtga cggaaatagt agcccaaaag atctaaaaaa
      721 accattcctg attacagtag gtataacttt tctgatttta tttcagccaa agattgctgg
      781 agcattgatg atcctttcga ttgcgtgggt catattttgg gcagcggcgg ttccatttaa
      841 aaaagggatc tatctaatcg ttactttttc tgcattgctg attggagcag caggcggggt
      901 attatattta ggaaataaag gttggcttcc acagatgttt aatcatgctt atgaaaggat
      961 cgcgacttta agagattctt ttatcgatag tcatggtgcg ggctatcaaa tgacccattc
     1021 gttttatgca ctatataatg gtggcatatt tgggcgggga ttaggaaata gtatcacaaa
     1081 aaaagggtat cttccagaga cagaaacaga ttttattttt tcgattatta cagaagagct
     1141 tgggctaatc ggtgcattgt gtgtgctttt cttattgttc tcattatgca tgagaatctt
     1201 ttgtttaagc agtcgctgta aaaatcagca agctggattg tttcttttag gattcggaac
     1261 tttgctattc gttcagacga taatgaacgt gggaagtatc gcggggctga tgccaatgac
     1321 tggtgtgcca ttgccatttg ttagctacgg aggaacaagt tatctgatcc tttctttagg
     1381 aatcggaatc acgttgaata tttcttcaaa gatacaggcc gaagagttgg cgctctatcg
     1441 acctgaaaaa caataaagag aaactgagaa actctcagtt tgaattatca taaagtacaa
     1501 tcttttattt ttcaagcgag aggttctttt agaaagagga attcgcaatg aaagtatctc
     1561 aaaaaatcat atta
//
LOCUS       DANKAY010000238         1564 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000238 DANKAY010000000
VERSION     DANKAY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            37..708
                     /locus_tag="PWY98_003061"
     CDS             37..708
                     /locus_tag="PWY98_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2038731.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          568 a          243 c          213 g          540 t
ORIGIN      
        1 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
       61 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
      121 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
      181 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
      241 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
      301 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
      361 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
      421 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
      481 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
      541 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
      601 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
      661 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
      721 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
      781 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
      841 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
      901 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
      961 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
     1021 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
     1081 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
     1141 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
     1201 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
     1261 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
     1321 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
     1381 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
     1441 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
     1501 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
     1561 tgcc
//
LOCUS       DANKAY010000239         1558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000239 DANKAY010000000
VERSION     DANKAY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            132..344
                     /locus_tag="PWY98_003062"
     CDS             132..344
                     /locus_tag="PWY98_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038732.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="PWY98_003063"
     CDS             506..1024
                     /locus_tag="PWY98_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDL2038733.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="PWY98_003064"
     CDS             1031..1543
                     /locus_tag="PWY98_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038734.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          613 a          181 c          242 g          522 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaa
//
LOCUS       DANKAY010000240         1536 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000240 DANKAY010000000
VERSION     DANKAY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..>1536)
                     /locus_tag="PWY98_003065"
     rRNA            complement(<1..>1536)
                     /locus_tag="PWY98_003065"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DANKAY010000241         1522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000241 DANKAY010000000
VERSION     DANKAY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            12..1478
                     /locus_tag="PWY98_003066"
     CDS             12..1478
                     /locus_tag="PWY98_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2038735.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          323 g          493 t
ORIGIN      
        1 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
       61 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      121 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      181 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      241 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      301 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      361 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      421 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      481 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      541 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      601 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      661 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      721 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      781 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      841 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
      901 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
      961 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1021 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1081 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1141 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1201 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1261 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1321 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1381 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1441 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1501 ttgcttattt gcagcagttc ct
//
LOCUS       DANKAY010000242         1516 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000242 DANKAY010000000
VERSION     DANKAY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="PWY98_003067"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="PWY98_003067"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2038736.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          526 a          193 c          248 g          549 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttatt
//
LOCUS       DANKAY010000243         1509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000243 DANKAY010000000
VERSION     DANKAY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(606..962)
                     /locus_tag="PWY98_003068"
     CDS             complement(606..962)
                     /locus_tag="PWY98_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2038737.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="PWY98_003069"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="PWY98_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2038738.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          465 a          274 c          216 g          554 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaat
//
LOCUS       DANKAY010000244         1503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000244 DANKAY010000000
VERSION     DANKAY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            104..754
                     /locus_tag="PWY98_003070"
     CDS             104..754
                     /locus_tag="PWY98_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038739.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            941..>1503
                     /locus_tag="PWY98_003071"
     CDS             941..>1503
                     /locus_tag="PWY98_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2038740.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          487 a          179 c          298 g          539 t
ORIGIN      
        1 tcagtgataa tcataagttc ataaatagta attgggatgt atggttggta tacgttatac
       61 ctgaacttga actatcaaga tttggtttag aatttgagaa tcgatgttta taaagggagg
      121 gcaaaaaatg tgctttttta caacttttac tatcattatg attgcagtac tttttatttt
      181 tctagatata gcaaagcgaa atacagcatt tttattatat cgggttttac ttcgagcagg
      241 tctcataaca tttatatcga tcgttggatt ttttctattc acagttattg tatttatttg
      301 gagaactaca gcaccaccat tgccagagat tacatatgga gaatttccgt ttagtcttga
      361 atatgaaata gatgaggaaa gaattttgat tgaagatacg gtgattgcag aatttatagg
      421 aagttttaga gggaatgtga caactcaacc tagacgaagt tggagtataa aacttttaaa
      481 taatagtgga gagttgaaag catttcatta tgaattctta ataatagaaa gtgaagatgt
      541 aacaatcact ttaagtagtg gctctgctgg atattttatg aatgatggtg gaagcaagcc
      601 acagctcaca gtccgtgatc gtattgcagg ttttatgcat ttttctcact atgacttaga
      661 agaagctcgt aaaatacttg cagaatatgg gatcacatta atcaattggg aatatgcgcc
      721 accgataaga aattttttca atgattatta ttagtcttat aaaaaacaga atgtaaaatt
      781 tatgtgcgcg agtatttagt tttccatttt ttgtttgata gatatatagc attgttgaag
      841 catgggatag tggtgccaat tttatcaatg aggttggtca ggcagttaac ataattggtg
      901 atgctataaa tccatttaat tggaattgga ggaattaggt gtggaaacag tgagattatg
      961 gatattcatg aaaaattttc gttcggcttt gagatatgtt attctggaat caaaatcagg
     1021 ttattatctg attgacacag atcactatac tttttgggga ggctaccttt ttccgatttt
     1081 taattggata atcccccata aggttatgaa aattaacttg cctgcagaag aaataaatag
     1141 attaatactc aacaaagatt tacaaaggaa aagagagaaa gaacattcta atgttgcttg
     1201 gataggtcca gcatttgcag tagtgacttc ttcaattgtc ggtcctatgc tagagagtat
     1261 ttatagtgat ttttctattg gaatacgtta tttatttgtt actttaagta tagctgttct
     1321 tttgtgcata aagggttatg ttagcaagaa gaataaagat attttaacac taatcggtgt
     1381 aaacaattta tctagtacta aaattatact gtttcctgaa tctataaaag aaggatcaag
     1441 gttgttgata atttttataa tgcttatact gttttctgtt ttatttgggg tgatgtttat
     1501 aga
//
LOCUS       DANKAY010000245         1417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000245 DANKAY010000000
VERSION     DANKAY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(150..1337)
                     /locus_tag="PWY98_003072"
     CDS             complement(150..1337)
                     /locus_tag="PWY98_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2038741.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DANKAY010000246         1383 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000246 DANKAY010000000
VERSION     DANKAY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..360)
                     /locus_tag="PWY98_003073"
     CDS             complement(<1..360)
                     /locus_tag="PWY98_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038742.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYY"
     gene            complement(377..>1383)
                     /locus_tag="PWY98_003074"
     CDS             complement(377..>1383)
                     /locus_tag="PWY98_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2038743.1"
                     /translation="EMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRER
                     LYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNL
                     LSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSI
                     IVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKT
                     KIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISK
                     RKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS
                     "
BASE COUNT          482 a          234 c          153 g          514 t
ORIGIN      
        1 gtaataatct tcccagcttt ctctttcaat taactgatcc aatttttgat aatccataaa
       61 ttcatcatgt atcaaaaaga ataaataact tgatatttta gcatctagct taatcagatt
      121 aaataatgtt tgtttaaaat tgctaccatt aatatgactg atagattctc tagcttcttc
      181 acataaactt tttactttat taattactac tttgtcatat tcatttaaca cactattatt
      241 accattactt agacgctcag attctacgaa acaactcaaa tcagaaattt cccaaaaaaa
      301 ttttttgtta aaatccaatt tcctcaactc ctccaaacta cgtaaatgat atagtctcat
      361 aataaaataa aacctctcag ctaatgttgt tgaaattgtc cactaaacct aattgatatt
      421 gcttaggaga tacacctgcg tactttttga atactctcga aaagtgtgaa gaatctgaaa
      481 atcctaaacc taaagaaact tcactgatag tatggtttga tttcaacatt attttagccg
      541 tagaaatttt tcgcttagag atataattat aaatcgtaat atgcatttca ttcttaaagc
      601 tttctcttaa tttagtttca cttacagaaa aataattagc tatttccttt acagtaattc
      661 ttttatagat atttaagccg atgaattgaa cgacagaaga aattaaggga gaaagatgct
      721 ctatcttagt ttttccgatt tccttagtat aataaacaat cccagaatcc ctcactttta
      781 agacctcagg taacgataca gctaattcgt tttcttgtat caaagcatct ctactttgat
      841 acgcttctat tatatccatt cctaatgtta tagacgtttg tgctaacttt tcaaaaacaa
      901 tgatagaata atttttttca gaccgaatcg tatctccact aacacttgga attattccat
      961 tactcagatt aaaaaccatg ttttctaaat ttcgaatatc tccattttta acatattcca
     1021 gtattttatt ttcataaaga aacagatata tttctggatc gtaattttgt gataacagat
     1081 tatctatttt gatttgctcg atagttttac ttagattttc tgtatattca attaacggtt
     1141 catggaatag atcttctatt tttccagtaa aaaaataatg aactaaaatc agaatatctc
     1201 gaatgtctcc taaagaatac aaagtcaaat tttgcaatga attatagagt cgttctctat
     1261 cagccattgc aacattcaaa ttgttcattt ttagtttaaa aaaaacttta tcaatattat
     1321 ttgctctaaa tggaccaaat aagtaatatc tggttttaaa catgtataga agaaacatct
     1381 cat
//
LOCUS       DANKAY010000247         1366 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000247 DANKAY010000000
VERSION     DANKAY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            41..1314
                     /locus_tag="PWY98_003075"
                     /pseudo
     CDS             41..1314
                     /locus_tag="PWY98_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          450 a          279 c          241 g          396 t
ORIGIN      
        1 agaattgaat caccacaaca caatcaaagg agcgttctgt atgcctgatc ttcatcttat
       61 caaactatta ctaggaatca aagatggaaa cattcaaatc actcatgtcg agaagaaaaa
      121 aatacattca atcaacagta tcgttgtttc tgccacgctc attaaaacga ttcagccctg
      181 tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt aagaacggaa agaaaaaatc
      241 gctggtcctt ctcaataagt gtgcgaatca acggacttat cttgctttgg caaagcaacg
      301 gtacctctgt cgtggctgtc acacctattt tacggcgaat acctacattg ttgaaaaaaa
      361 ctgttttatc gccaaacaag tacattataa aatattggag gaattgactg aaaaacaagc
      421 aatgacgacg atttctaaac attgcggcgt ttcttggtca accgtttcgc gaacactcgc
      481 ttctctgctc cccatgacaa acgtgaaaat aaattggttg cctcgctgtt cattggtaga
      541 tgaatttcgt tcattgaaaa atcaagtcgg tccctattct ttttcttgta tggatggcga
      601 tactgggaaa ttactggata ttcttccttc acgtaagaag aaagatctca tttcttattt
      661 tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata ctcgtaacag atatgaacgc
      721 ctcttatgct ttgctgataa aagagtgctt tcctcatgcc aaactcgttg tggatcgctt
      781 tcacatcgtc aagcacctca ttcgcagttt taaggatatt tgactatgag tcatgaaaag
      841 cttcgaccga aacgatccaa tacaagccaa acactatcga caagtgaagg ctttatctcg
      901 acttctgatc aaacgacagg acacgcttgt ttatgataaa tggacaaaat ggcgtaattt
      961 tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt ctactttcta cttctgatga
     1021 actacgaata gcttatgctt actatcaaga aattcttcaa gctttttatg acaaagaagc
     1081 ggacatcttc tttcagcttg taaaaacaat gcccaagtcg gttccaagag aactgcatca
     1141 tataaaaaaa gcgtttataa actatgaatc cggcattcgt ttagccttag agttgcctta
     1201 ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa gcattgaaac gaatagctta
     1261 cgggttagga tgagaaccag aattttctta atcaatcatc tgatcactta ttaaacataa
     1321 aaaaacatct aaagcagaaa atcaattcta ccttagatgt tcttat
//
LOCUS       DANKAY010000248         1358 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000248 DANKAY010000000
VERSION     DANKAY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            82..>1358
                     /locus_tag="PWY98_003076"
     CDS             82..>1358
                     /locus_tag="PWY98_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2038744.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          462 a          262 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatca
//
LOCUS       DANKAY010000249         1324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000249 DANKAY010000000
VERSION     DANKAY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            278..820
                     /locus_tag="PWY98_003077"
     CDS             278..820
                     /locus_tag="PWY98_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038745.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="PWY98_003078"
     CDS             976..>1324
                     /locus_tag="PWY98_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2038746.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DANKAY010000250         1320 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000250 DANKAY010000000
VERSION     DANKAY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..115
                     /locus_tag="PWY98_003079"
     CDS             <1..115
                     /locus_tag="PWY98_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2038747.1"
                     /translation="SSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(201..1295)
                     /locus_tag="PWY98_003080"
     CDS             complement(201..1295)
                     /locus_tag="PWY98_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2038748.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          399 a          279 c          205 g          437 t
ORIGIN      
        1 ttccagtgtc agcaatcaac gcaatcatat tccaagaaaa tcattgaatt acagaacacc
       61 aatcgaaata tttttgagct atgtacaaga agcattttat tctagcttaa tttgacaaat
      121 cataacctat aaaaaaacaa aataaaaaag cagaaataac tggctatttc tagcggatta
      181 tttctgcaat aactaattat ctatttttta ttttcaatta atttaaagct tgaccatggt
      241 ttcaaaaagt caaggaggaa taattcgtca tcggagattc ttcctacaac attgacacgt
      301 ccgtcgtttt tcatatcctt caaggcgatt tgtgtttctc ctttgtattg accgtaccct
      361 tcattgtcaa tcagtacatc cccatgatgc atatctcgcg tattatgagg cgggaaatct
      421 ttatctttgt agtatacacg agtcatcgtt gaacgaagga tatattctga gcgatcccct
      481 cgataactat gaaggttatc aaacaagcag attcgttcgt tttctgtaat accttcttcc
      541 gtatctactt tcaatgttgg ataatccgcg ttaaatgctt ctgccatttc tttcaattct
      601 tcttcacttg cgtacgcatt tccaatagaa atatcatcaa tcaatcctgt aaggattaaa
      661 tgcttcactt gtgtcgcaat ttcaagatct cggtggtctt ccagcgtgca caagccatct
      721 tgcgtaggcc aagggccaaa ctcagcagat tgcgaattga cgaatgccat cgtattcaaa
      781 ttatattttc tgaatttttc tgaacaaaaa acaaagtgtt cgtatcctaa accagagtat
      841 cgatgaggat aaaaattatg agaacctaat aaattatccg tgtttggcga ataactcatg
      901 atgttgtcaa cgtaattcgt gcccgaactc atattgatct cgattttgat accaaaagga
      961 ttgcgcgtca tttttgcttc ttctgctcct gtgaatccga tatctaagcg gaccccgtag
     1021 gcacccatct tatggaaaaa agataagtca tcgtaagaaa tctccaactg ttcaaataac
     1081 gctggattga tatcaaccat tacttccatg cctaggctat tcgcgtaatc aactacttct
     1141 ttaaattctg aaagaacttt ttctttatca tcattgatct gcagcaaact ggtaaaaacc
     1201 cgtttatacc catacttatg agctaaatcc aagtacgctt tgtccttttc aaatgttgac
     1261 cgttctggat aaatcgagat tcctaatttt cccatattac catttcctcc tgcatctttt
//
LOCUS       DANKAY010000251         1312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000251 DANKAY010000000
VERSION     DANKAY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(268..720)
                     /locus_tag="PWY98_003081"
     CDS             complement(268..720)
                     /locus_tag="PWY98_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038749.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(727..1275)
                     /locus_tag="PWY98_003082"
     CDS             complement(727..1275)
                     /locus_tag="PWY98_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2038750.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          476 a          257 c          127 g          452 t
ORIGIN      
        1 gactaataaa acaatatttg taatcaatcc ttgcactaaa gaattaaaag gaagactatt
       61 taaaacaggg tgtgcataac tataacatgt gaagtagtta ttttgaactt taacttttaa
      121 aaactttaaa aataatctta tttgttcttc taaacaatca tctatactca tataatcact
      181 ccactaatta atctccccaa aaagacacag cattaagaga tataccaaca caacagtgca
      241 aataactcta catcttcctt cgcaacctta tttcaataaa ccataaaaat tattttttaa
      301 tagaaactga aaattaaaac tagaaaaagt agttttaccg aatgtaagag catcatcacc
      361 atcaatatag aaatacattc ttttccagtt acatttaatt tttaaacaaa attctcccca
      421 aaaaggaact ttttctagtg aaaacttggt aatctctttg taattaattc ttgttaaaga
      481 ataattaaac acttcatttt tacttgttat tttttcattg actataacta gttcatctgg
      541 taaaaaaatt aaccaacaaa catcgacaaa cagtaaatga tataatccat ttccacctcc
      601 aagcatattc aatactggtg aacatgcctt agaaacacga atatagttat ggtttaaata
      661 gatattttct ttaaacattg cttgtttaaa agccatatgc tcttgttcta aacttttcat
      721 aaacttttac tctaatgcac agctagcagc agctgctgca attcctgcta caatatttac
      781 cttagctaac tctttagctg cagcttttcc taattgtttt tctgcaattt ttaataataa
      841 atccgcagct ttttcaaaat taaaagcttt taatgcttca ataaaatctc cctgcgtacc
      901 tatagcccaa gccactcctc ctccaggaag agaattaatg gctacacatc cagcatatcg
      961 tttccaatca actttctcca ttctaggaac aataatatgt tcataaaaga ctcttgctgc
     1021 tcttccttgt tcgttatctt gactgattcg ttcacgaata gcaccaaaat cctttatatg
     1081 gtatacacca tgttcgtcta aaaaaccaac tgtatcaaaa taaaaagtta attcatctac
     1141 taatttattc tttatttcta aatcaactgt attcactttt tctgtggcat acacacttgt
     1201 tccagtacta acaaaagtac ttaaacctaa taagaaaaca cctaaaacac caaatatttt
     1261 aattattctt ttcataattt ctcctccata aaaaataagt tactttttaa at
//
LOCUS       DANKAY010000252         1302 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000252 DANKAY010000000
VERSION     DANKAY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            52..>1302
                     /locus_tag="PWY98_003083"
     CDS             52..>1302
                     /locus_tag="PWY98_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2038751.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DANKAY010000253         1291 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000253 DANKAY010000000
VERSION     DANKAY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1120
                     /locus_tag="PWY98_003084"
     CDS             <1..1120
                     /locus_tag="PWY98_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2038752.1"
                     /translation="LPVEVNYWDGTTKQYGDTDTPPQIKINIHEEIPMKEITSNASLA
                     LGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPKKEKHTKQKNKEDIHAH
                     YDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHILDKLFIKEGETLLDIGC
                     GWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDKCRVLLMDYRELKGETF
                     DHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQQGGATNAWINKYIFPG
                     GYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFHQVQDQVTKEMGDSFYR
                     MWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT          462 a          217 c          243 g          369 t
ORIGIN      
        1 gctccctgtc gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac
       61 tcctccccaa atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa
      121 cgcttctttg gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat
      181 ccaagcattg atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta
      241 tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca
      301 tgcgcattat gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta
      361 ttcttgtgct tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt
      421 ccatcatatc ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg
      481 tggctgggga acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat
      541 cacactaagt gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga
      601 agataaatgc cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca
      661 tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa
      721 agtggtaaaa gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca
      781 acaaggcggt gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc
      841 tggtgttact gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga
      901 aagcttacgt cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt
      961 ccaagatcaa gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt
     1021 acaagcttgt gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt
     1081 tcaacctgga aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt
     1141 aaagactatt taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta
     1201 acttctagga ttgtctctaa agcttagttt ctttttcttt atattttcat aataaaagtt
     1261 tggttcttta atttttaaac atgacagaaa a
//
LOCUS       DANKAY010000254         1275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000254 DANKAY010000000
VERSION     DANKAY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(31..1203)
                     /locus_tag="PWY98_003085"
     CDS             complement(31..1203)
                     /locus_tag="PWY98_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL2038753.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DANKAY010000255         1265 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000255 DANKAY010000000
VERSION     DANKAY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..760
                     /locus_tag="PWY98_003086"
     CDS             <1..760
                     /locus_tag="PWY98_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2038754.1"
                     /translation="KKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAES
                     TTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKD
                     GYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQ
                     DPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMD
                     VVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(875..>1265)
                     /gene="fabZ"
                     /locus_tag="PWY98_003087"
     CDS             complement(875..>1265)
                     /gene="fabZ"
                     /locus_tag="PWY98_003087"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2038755.1"
                     /translation="YPICYIDYVDTLEPGKKIVATKNVTINEDFFQGHFPDNPVMPGA
                     LILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVPGDVLKCHFEITKMKGSV
                     GTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          417 a          247 c          244 g          357 t
ORIGIN      
        1 gaagaaaaat aaagttattt taagtattag tttagcagca gcattgctgt ttgctgctgg
       61 ttgttctgca aacaacacga cttcttctag ttcgtctaca gaaagttcaa aaagtacatc
      121 tactgcggaa agcacgactg ccagtagcgt cgatttgaac gcattggaat taccacaatt
      181 gacgaaagat gtcgccgaaa atgaagatct ggtcgaaatg gtgacgaata aaggcacaat
      241 caaaatcaaa ttatttcccg aacacgcacc aaaagcggtt gaaaacttta tgacacatgc
      301 aaaagacggt tactacaacg gactgacttt ccatcgcgtc atcaaagatt tcatgatcca
      361 aggtggcgat cctaaaggcg atggcactgg aggggaaagc atttggggag aaggatttga
      421 aacagagatc aacaaccacc tttataatat aagaggtgcg ttatctatgg ctcgctcaca
      481 agatcctaac agtaacggca gccagttctt tatcgttcaa aatacagatg atatgcatga
      541 tggtctttta aaagacgact atcctcaagc aatcatcgac gcttataaaa acggcggtta
      601 tccttcacta gatggccatt atactgtctt cggccaagtg attgaaggaa tggatgtcgt
      661 ggatgctatt gcaaatatcg aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat
      721 cgataaaatc aatatactgc aagaagcaaa acaaaaataa aaaaataacg tctgaatcct
      781 gcatagatga caacgtatgc taggattcag gcgtcttctt tttacataaa aagcaagtaa
      841 gcttcctttt gttttgctat atcaggtttt atagtcaagc attttggttg acgatgaacg
      901 tgaactcgca ctcacagact tttttatcat ctacatatgc tgcagataac gctgtaccga
      961 ctgatccctt cattttggtg atttcaaaat gacatttcag cacatctcct ggaacaactt
     1021 tttgacggaa tttggcctta ttgatcccac cgatataagc tctttttcct agaaactctt
     1081 ctgattttag gataagtatc gaacctgctt gtgccagtgt ttctagaatc aacgcacccg
     1141 gcatcactgg attgtcagga aagtgacctt ggaaaaaatc ttcattgatt gttacatttt
     1201 ttgtcgcaac gatctttttt cctggttcta aagtatctac ataatcaata taacaaatag
     1261 gataa
//
LOCUS       DANKAY010000256         1203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000256 DANKAY010000000
VERSION     DANKAY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..552
                     /locus_tag="PWY98_003088"
     CDS             <1..552
                     /locus_tag="PWY98_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2038756.1"
                     /translation="KTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFDR
                     WREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVRHES
                     ISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEG
                     ALIIATVDDPKQNVFTLEKVGVI"
     gene            552..1169
                     /locus_tag="PWY98_003089"
     CDS             552..1169
                     /locus_tag="PWY98_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038757.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          445 a          152 c          262 g          344 t
ORIGIN      
        1 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
       61 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      121 gaatttgatc gctggagaga agaacaggag aatagtttcc aagattggag agaaggacaa
      181 gaatcagatt atttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      241 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      301 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      361 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      421 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      481 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      541 ggggtgattt gatggttaaa gtaaagagaa tgatggagac tgatgaatct ggtgttaaac
      601 gtcaatttat gccaattaca catgtttctg cagttttagg cttagaaaaa atgatatcag
      661 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
      721 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
      781 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
      841 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
      901 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaagg
      961 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1021 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1081 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1141 ttacttttga aaaaatcggg gaggtataga aaatgacaga tatcgtaaaa ataaaacaaa
     1201 gcg
//
LOCUS       DANKAY010000257         1198 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000257 DANKAY010000000
VERSION     DANKAY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>1198
                     /locus_tag="PWY98_003090"
     CDS             <1..>1198
                     /locus_tag="PWY98_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2038758.1"
                     /translation="FITIPIFVNITTQAEYGLINNFTSFVSIFSILVGLSLNSAINNA
                     SFDYKDDINEFMSSVLSLATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFAS
                     FVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSF
                     VLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGP
                     AAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLIT
                     MIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIG
                     TVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKIC
                     IIIIFVS"
BASE COUNT          377 a          152 c          186 g          483 t
ORIGIN      
        1 cattcataac tattcctatt tttgtaaata ttactactca agcagaatat ggtttgatta
       61 ataatttcac ttcatttgta tcaatttttt cgattcttgt tgggctaagc ctaaattcgg
      121 caattaataa tgcaagtttt gactataaag atgacataaa tgaatttatg tcatcggtac
      181 tttctctagc tacactttcg ttaattgtgt tcttattaat agggaacata tattttttgt
      241 tttcaaatgc tttttttgat atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt
      301 ttgctagttt tgtaattagt tatatccagt cttattttac gattaacttt aaccatttta
      361 aatatttgat tttatctttt atatctactt ttttgaatat tgcaatctct gtagctttga
      421 tgcagacatt atattttgat gataaatatg ttggcagagt agtcggcgga acactgtcat
      481 ttgtgttgat aggtttagta atttatttaa gaataatgat aaagggtgct catttttttg
      541 ataaaaaaca gtggagatac gctttaaaaa tctctttgcc gctaatccca cattccttag
      601 caaatatagt tttagcacaa ttcgatagaa ttatgattaa ttcttattta ggtcctgctg
      661 cggggggaat atattcctac attagtaaca taggtattat tctaagcgta atatggatgt
      721 ctactaataa cgcctgggtt ccatggttct atggtgagat gagtaaaaat aatgaaaaaa
      781 aagtgaagaa agttagtaac tattatttag tagtctttac tttgattacg atgattgtaa
      841 tggtagtttc gattgattta gcaaaattta tggctccgaa agactattat tctgggttat
      901 ctttagtggt tccgataacc ttaggctatt tctttcagtt tttatatagt ttaccagtta
      961 atgctgaatt ttatgagaaa aaaacatcgt ttattgcgat tggtacagta gcaagtgcta
     1021 tagttaatat actattaaac atgattttca tacctaggtt aggaataatt gctgctggat
     1081 acacaaccgt atttacgtat tttttacttt ttatttttca ttataatatc gctaaaaaaa
     1141 ttacgaataa acaattattt gacacaaaaa aaatatgtat cataataatt tttgtatc
//
LOCUS       DANKAY010000258         1193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000258 DANKAY010000000
VERSION     DANKAY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..1162
                     /locus_tag="PWY98_003091"
     CDS             <1..1162
                     /locus_tag="PWY98_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2038759.1"
                     /translation="IKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSV
                     SDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQPLP
                     EVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQVR
                     LVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRSKH
                     LKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDFLS
                     AIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLNNH
                     IKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          375 a          268 c          238 g          312 t
ORIGIN      
        1 cattaaatgg ggctggaaaa aagtatccat cttattgaat gatgtcagta actacaagac
       61 cattctgcgg attaacaaac agcgcttcaa atgtaaacac tgtggaaaga cttttttagc
      121 agaagattca gttagtgatc gtcgctgctc cattgctcgc agagtcaaac aagccattct
      181 tgagctatta agcgaacctc tttctatgtc tttgatcgca cgtatgaagc acatttctcc
      241 gaccactgtc attcgaatac ttcgcagcct tcggcctaaa accgtttctt tgaaccagcc
      301 gttaccagaa gtggtgtgtt tcgatgaatt caaatcggtg aaaaacgtct cgggagccat
      361 gagctttgtc atgatggacg gcaagaccca tcaactgatt gatattgtgg aaaatcggca
      421 actaaatcct ttacgcgact atttcttgcg ctaccctaga aaagtccgtg aacaagtacg
      481 tctagtggtt tcagatttct atacacccta tcgcacattg gtgaaagagt gctttccgaa
      541 tgcccgaatc gtcgccgatc gctttcatat tagccaacac atcggtcggg cctttaccaa
      601 ccaccgaatt caagtaatga agacctttaa aaaaagggat cgtcggtcga agcacttaaa
      661 gaaatactgg agactacttc aaaagaatgc gtgggaattg aaaggtcagc atcgctactg
      721 gcgtccgtca tttcgagatc atctgacaga agcagagata gtcgatcggc tattatctta
      781 tgatgactcc ttaaagcgag gctacgaagt ctaccaggat tttctttccg ctatcagaag
      841 acaagatgtt cctgaattcg tcgcgctcct aaaagaagac tacaaggagt tgccagagca
      901 ctaccaacca gtgttcacga ccttcaagaa atatcgaaca gaaatcaaac gagcgttgcg
      961 agttccctat tccaacggcc ctatagaatg tttgaacaac catatcaaag tactaaaacg
     1021 aattgcctat ggctttagaa actttcaaaa ctaccaagaa cgaatctttt tatatcgagg
     1081 aaaatatttt aagaaaacta agaacactac ccaactgact aaagctagaa caacaacacg
     1141 gctagacaag atagctgtct agccgtgtga gtatctcgat tattctattg aat
//
LOCUS       DANKAY010000259         1172 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000259 DANKAY010000000
VERSION     DANKAY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..454
                     /locus_tag="PWY98_003092"
     CDS             <1..454
                     /locus_tag="PWY98_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2038760.1"
                     /translation="VGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWA
                     NITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTG
                     DVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            464..>1172
                     /locus_tag="PWY98_003093"
     CDS             464..>1172
                     /locus_tag="PWY98_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2038761.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTD"
BASE COUNT          421 a          128 c          230 g          393 t
ORIGIN      
        1 agtaggaatg ttcttttcag caatagtata tttgatctta tttttgataa aagctagtaa
       61 tatgtatgta ttcttaattc ttagttcggt tggctatatg ggtttgggtg tgtttaatac
      121 tgttatttgg gcaaatatta ctgatgttat tgatgatcaa gaagtaaagt ctgatcaaag
      181 agaagatggg accgtatatg cggtatattc ttttgctaga aaaataggtc aagctctagc
      241 tggaggcgtc ggaggttgga ctctttctat tataggctat gactctttag caaatgtaca
      301 aacgggagat gttttagaaa agttatataa cgcttcaaca ttaattccat caatttgttt
      361 tctgattgta ggattaattt tatttttcct ttatccatta tctaaagata aagtcttaga
      421 aaattgcgag cttttaaaac aaaaacataa ataggagaga aatatgaata attggatatt
      481 ttgtattgat tcagatggct gtgttatgga tacgatgact tataaacaca atctcttttt
      541 cggaccaatt gctgcagaga tttttgatgt tcatcataga gaaaaattcc tacaggattg
      601 gaataatatt aatttatatt cagagactcg tggaataaac agatttttag gtttagttaa
      661 aacattagaa aaaaatgatg taacatcgat taatcgatta aagcactggg tatctctaac
      721 aaaagaattg tctaatccat ctttgaagag ggaaatagaa tattctcagt cagaggattt
      781 attaaaagct ttaaaatggt cggaagaagt caatcaacag attagtcaag caaaaggatt
      841 agataaaccc tttttaagtg ctagagatac tataaaagct ctgaaaaaat atgggaagat
      901 aattattgta agctcagcta ataaggaagc tgttcaagaa gaatgggaaa gacatgaatt
      961 attatcatta gttgatgaac tgtgttgtca agataaagga aaaaaagaag atattattcg
     1021 aagtgtaatt gaaaatggtt gtgatttgga taagatatta atgattggtg attctcctgg
     1081 tgatctagaa gctgcaaata aaaataaagt tttcttttat cctattttgg tcaataaaga
     1141 aaaagaatca tgggaaaact taagaactga tg
//
LOCUS       DANKAY010000260         1171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000260 DANKAY010000000
VERSION     DANKAY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(167..240)
                     /locus_tag="PWY98_003094"
     tRNA            complement(167..240)
                     /locus_tag="PWY98_003094"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Ile,seq:cat)
     gene            complement(795..1109)
                     /locus_tag="PWY98_003095"
     CDS             complement(795..1109)
                     /locus_tag="PWY98_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038762.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          447 a          181 c          179 g          364 t
ORIGIN      
        1 aacttttcaa atatacagaa gtataactat gtgaaacatc ctttcattaa tccataaaag
       61 gatacataaa aaagccactc atttgagtgg caatgaagaa aagctttagc ttgtataata
      121 ctcttcaaaa aattctaaca cagaacgatt caattggcta cattaatgta ccctgtagga
      181 ctcgaaccta cgaccggacg gttatgagcc gtctgctctg accaactgag ctaagggtag
      241 tggttgttgc cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg
      301 ttatcgcata tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata
      361 tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta
      421 ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca
      481 aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt
      541 ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg ctgatggaag
      601 gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat tataaactct
      661 agagaaatta attcatccta acataaaata attgttcact caaaagtttt tctagaacaa
      721 ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt cctgtactaa
      781 aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag tagttgtgta
      841 ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga aacgtctata
      901 acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc tccgtaaaaa
      961 atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt tctaaaacct
     1021 atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa tattgctatt
     1081 attaagataa aattactgta aaaatccaaa agctatccga gagtataaaa tattttgtgt
     1141 aaatgaaaaa atccatacaa aaaaggaagt c
//
LOCUS       DANKAY010000261         1160 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000261 DANKAY010000000
VERSION     DANKAY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            17..277
                     /locus_tag="PWY98_003096"
     CDS             17..277
                     /locus_tag="PWY98_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038763.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(639..878)
                     /locus_tag="PWY98_003097"
     CDS             complement(639..878)
                     /locus_tag="PWY98_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038764.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          408 a          163 c          217 g          372 t
ORIGIN      
        1 taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat atatttgcca
       61 acctattggc ttgaaacaaa aagtggttgg agaagtaatc agtaaattag aaaattgtgt
      121 tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg
      181 aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat atctatataa
      241 ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa gtgccttcaa
      301 aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt gttcatgatt
      361 tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc ctggtggaga
      421 aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca aactagtagt
      481 tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat caggatcatt
      541 tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag gagtaaacgc
      601 gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa
      661 taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga aacttttata
      721 tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc gatactattc
      781 gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc agtaagtaac
      841 aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact ccttatagta
      901 tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact atcaagtctt
      961 ttatagtatc tattagtctt agtaatacat atatggagag ggtgatatcc cagttcaaac
     1021 cagatacatt ggttaccatc tgtattaacg attatgcctc taaacgcttt ttttatgaaa
     1081 aaatttataa gtgaaaacgt aggtatgata ttatctagag gtaatttttt tgggtattta
     1141 aatagttgct aggaggatag
//
LOCUS       DANKAY010000262         1137 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000262 DANKAY010000000
VERSION     DANKAY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..355
                     /locus_tag="PWY98_003098"
     CDS             <1..355
                     /locus_tag="PWY98_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038765.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="PWY98_003099"
                     /pseudo
     CDS             615..910
                     /locus_tag="PWY98_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="PWY98_003100"
     CDS             985..>1137
                     /locus_tag="PWY98_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2038766.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DANKAY010000263         1127 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000263 DANKAY010000000
VERSION     DANKAY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..390)
                     /locus_tag="PWY98_003101"
     CDS             complement(<1..390)
                     /locus_tag="PWY98_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2038767.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTA"
     gene            complement(387..623)
                     /locus_tag="PWY98_003102"
     CDS             complement(387..623)
                     /locus_tag="PWY98_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2038768.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(783..1067)
                     /locus_tag="PWY98_003103"
     CDS             complement(783..1067)
                     /locus_tag="PWY98_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038769.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          355 a          205 c          174 g          393 t
ORIGIN      
        1 tgcggtttgt cgtaaataac tggttatctt ggactttagt gaatcgaaat ccaatgaact
       61 agtggtaatc attatgataa aatcaacagc ttcttgacga gaaaaagcaa ctggacaatt
      121 attcaattga agaaacaaca acatggcaac aagagcagtt cgtttattgc cattatgaaa
      181 aggatgtttt ttagctaaat taattgctaa aatcgccgct ttttcataga tttcaggata
      241 gagttcttga ttgaatactg cttgtgaagg ctgtttgacg gccatatcta atgcatttgc
      301 atcttttaca ccgatcattt cgcctggcga gtgacgtaaa ataactttcg cgttgatttt
      361 gataatttct ttagtcgaaa gatacctcat aaatcaacca aatcttttaa cgcgtcttca
      421 tattgattca tgatcgaatc aactaatgaa tcaatatcag gttcttcttg tgctttgatc
      481 attcgtacag aaccatcaga ttgaaaaaca taactgattg cgtcacctgt ctgcacaccc
      541 atgaattctt ttacttctgg tgggatagtt gttacatcgc tattgccaga ttttctaatt
      601 ttatactttt tcattttttt catatttcgc caccctctat accattctca ttctatagta
      661 agtacattat atccaagtat atacttttaa tcaatgtata tacttttatt ttgctttttc
      721 ttacgaaaaa atgacaaaga aaaaagtttg tgacatgact tttgtcacaa actctcgtat
      781 tcttaaccaa aatattgatg aaatacttgt gtaataactg gtagaaaata aataatgatc
      841 atagccgcta ccaccaccaa caattgtttt cgctgtttct ttcgctttag catgcttgtc
      901 gttttatgaa aaaaataagt agcgctttta gctcctgcag tcaaagtcga taaaatcaac
      961 gttggtgaat atgctgtttg attgtgcgtc aatgacaaca acttcgtccc gtaatcagga
     1021 tactttagaa atgcttggta cacagccaca atcgtgattg ctcccataag ataatttaaa
     1081 aatgaatgca cttttttcaa ataaaaaaac tccttgctat ctatata
//
LOCUS       DANKAY010000264         1118 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000264 DANKAY010000000
VERSION     DANKAY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>1118
                     /locus_tag="PWY98_003104"
     CDS             <1..>1118
                     /locus_tag="PWY98_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2038770.1"
                     /translation="DLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGSSRDKN
                     YLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTE
                     VGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQ
                     NPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGK
                     VYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNG
                     LVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDD
                     RLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIG"
BASE COUNT          383 a          147 c          232 g          356 t
ORIGIN      
        1 gatttacgaa aaaaaccata taatttagat gaagatgcta tttcttggat tgaagagacg
       61 attgattcta tgactttgga agaaaaaata ggtcaattgt ttgttaatat gggatctagt
      121 cgtgataaaa attacttgag taatatggtc aatcagtacc atattggagc tgtacgatat
      181 aatccaggaa ctgctgatga ggtatatgat caaaactata tcttacaaac agaaagtaaa
      241 attcctctat taattgctgc taatacggaa gcaggtggta acggagcttg tacagatggt
      301 actgaagttg gtgtagaagt aaaaattggt gcaacaaatg atgcgaaata tgcttatgaa
      361 ttagggagag tttctgggat tgaagcatcg gctattggat gtaactggag ttttgctcct
      421 attgtagata ttaatagaaa ttggagaaat cctatcattt ctagtcgatc ttttggacaa
      481 aaccctgata aagttttaga acttagttta gcttatatga aaggtattca ggagagtgga
      541 attttgcctg cagctaaaca tttccctggt gacggtattg acgaacgaga tcagcatcta
      601 tcttttagta ttaatagttt aggtacggaa gaatgggata attcttttgg aaaagtatac
      661 tcaggcttaa tagaagctgg attaccttca attatggcag gtcatatcca tatgccagct
      721 tattcaaaac attttaatcc tgatctgaaa gatgaagatt tacttcctgc aactttatca
      781 aaagaattaa tcactgactt acttagagga aaactcggat ttaatggatt agtagttact
      841 gatgctagtc atatgttagg aatgactggg gcaatgaaac gatcagaatt attgcccact
      901 tctattgcag ctggatgtga tctatttcta ttttttaatg atccagatga agactttaat
      961 tatatgatgg aaggctatcg taaaggaatt atcactgatg atcggttatt agatgcctta
     1021 aaaagaattt tagggttaaa agctaaattg ggattacaca aaaaagcaaa agaacaaatt
     1081 ttattaccta agcaagaagc tctggaaaaa attggttt
//
LOCUS       DANKAY010000265         1095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000265 DANKAY010000000
VERSION     DANKAY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            1..988
                     /locus_tag="PWY98_003105"
                     /pseudo
     CDS             1..988
                     /locus_tag="PWY98_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          383 a          199 c          220 g          293 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgcac
     1081 cttataaaat ttgtc
//
LOCUS       DANKAY010000266         1073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000266 DANKAY010000000
VERSION     DANKAY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>1073
                     /locus_tag="PWY98_003106"
     rRNA            <1..>1073
                     /locus_tag="PWY98_003106"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DANKAY010000267         1067 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000267 DANKAY010000000
VERSION     DANKAY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="PWY98_003107"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="PWY98_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2038771.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DANKAY010000268         1064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000268 DANKAY010000000
VERSION     DANKAY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(39..995)
                     /locus_tag="PWY98_003108"
     CDS             complement(39..995)
                     /locus_tag="PWY98_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038772.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DANKAY010000269         1042 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000269 DANKAY010000000
VERSION     DANKAY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..>1042)
                     /locus_tag="PWY98_003109"
     CDS             complement(<1..>1042)
                     /locus_tag="PWY98_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2038773.1"
                     /translation="ISSGASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAI
                     TANFVSLYFFGLRPVLYLGDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYN
                     RLPLPAYFHGFVPFLLILPIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVF
                     SMISYGGGLPGGIFLPILTLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIG
                     KAPLTAIILVTEMVGSLNHLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTAS
                     LKGARTTFDFPVTAESSLDGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDIL
                     VILTDEANLPRI"
BASE COUNT          374 a          216 c          206 g          246 t
ORIGIN      
        1 ttgattcttg gcaagtttgc ttcatcagtc aagataacca aaatatctcc tacattcatg
       61 atcgtatcac cgtgagtcaa gatttcctga tttcctctgc gtatagagat tagcagcata
      121 tttttaggcc aattgaagtc tcgtaccatt aatccgtcta aagaactctc tgccgttact
      181 ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg tctgagtatt tccaagtaga
      241 cgttccaata atgattcgta aattggatta ccgccgagaa ggtcattgac gatataagca
      301 acaagagcag ccaatcctaa aggcatcaga tgattaagag agccaaccat ttctgtcact
      361 aaaataattg ctgtcagcgg tgcttttcca atcgctgtga aataaccagc catggcaata
      421 acgacaaaat ccttgatgta gattggatcc atcccgaaat caaccataat gtttccataa
      481 aagctaccga ttaaagcccc caatgtcaat atcggaagga aaatcccccc tggaagaccc
      541 ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa acaaaccgat aatcataatg
      601 attggcaagg aagcatgtcc aaaagctaaa acgatttgat tgcctccgcc tagagtacta
      661 ggccataaat aaccaattgg caaaatcagc aagaaaggaa cgaacccgtg aaaataagct
      721 ggtaaaggca atcggttgta aaattttggc aaagccagca agacattctg ataaataaaa
      781 cctaagatgc ctagaagtac tcctaagatc actaaggaac cataatattt caacggcaaa
      841 gaaggaaggt cacctagata taaaacaggt ctcaagccga aaaagtaaag agagacgaag
      901 ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa gtggagaaaa ggagtgatgg
      961 atttcttcta aaacaaagag taatcctgcg ataggagcat tgaatgctgc tgcaagtccg
     1021 ctacttgcac cgctagaaat ta
//
LOCUS       DANKAY010000270         1030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000270 DANKAY010000000
VERSION     DANKAY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(56..790)
                     /locus_tag="PWY98_003110"
     CDS             complement(56..790)
                     /locus_tag="PWY98_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2038774.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          364 a          211 c          183 g          272 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa
//
LOCUS       DANKAY010000271         1030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000271 DANKAY010000000
VERSION     DANKAY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            121..>1030
                     /locus_tag="PWY98_003111"
     CDS             121..>1030
                     /locus_tag="PWY98_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038775.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGI"
BASE COUNT          350 a          127 c          140 g          413 t
ORIGIN      
        1 gaaacactac ttttgtaata taaaaaatat tttattaata ataaaattaa tcacatttta
       61 tatagtgact aatctagtgt ttaaaaaaaa taaatgatac tatatgtaat ataaaaaaac
      121 atgaggtggt atatgaattt gaaaaaaatt ttttctgtaa aaatatttat agtttatgca
      181 tttgttagtt tattatattt atttccagca tttaaaaaca tggaaatatt tggcttttat
      241 gatacggcat ttcacattaa tcgtgcgtta agtttagaat ctatttttag ctcccctatt
      301 aattttgaaa cgtttagatc atatggcatg caagtaaata atttttaccc atggttaact
      361 ctatatcctt tatttttatt gatcaagttt actaatttag caataggcta taaccttttt
      421 ctttatattg ttacgctcat tactctattt atatgtcatt atgtaatgta tgaaattacc
      481 aaaaaacatg ttactagctc ctttttcgct attatatata caacatcttc ttttaggagt
      541 gtagaaatat ttcttcgagg ggcaatgggg gaattactcg caatgtcaat acttcctctt
      601 attttactcg ggtttataaa gctttacgac tcaaaaaaag aatcttgggt tatgttggcg
      661 attagcatga ctttattaat ttacactcat gttctttctg tagctatgac tatggtaatg
      721 attataattg ctttaattat tcaattttat cggaaaaaga aaatagaaat cttcttaatt
      781 attaaattaa taaaagctgc tatagttact ttacttcttt cactagcgtt tataggtcct
      841 atgattgagc agacattgta tcaagatctg aatagacctt atgtggatat attacaaaaa
      901 agagcaattt atctgggtaa agagtttttg attggaagta tagattcaga attactatca
      961 tttggtattg gcttaacttt gcttatttgt ttaattatac taacatttaa ctttaaaaaa
     1021 ttaggaatct
//
LOCUS       DANKAY010000272         1023 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000272 DANKAY010000000
VERSION     DANKAY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            112..1002
                     /locus_tag="PWY98_003112"
     CDS             112..1002
                     /locus_tag="PWY98_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2038776.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
BASE COUNT          365 a          173 c          192 g          293 t
ORIGIN      
        1 atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta atataacttt
       61 tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta
      121 caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag
      181 atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga
      241 gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa
      301 acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatattagt
      361 tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac
      421 tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg
      481 aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg
      541 tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg
      601 gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg
      661 ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga
      721 gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa
      781 tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc
      841 ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc
      901 gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt
      961 aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt
     1021 tat
//
LOCUS       DANKAY010000273         1011 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000273 DANKAY010000000
VERSION     DANKAY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..196)
                     /locus_tag="PWY98_003113"
     CDS             complement(<1..196)
                     /locus_tag="PWY98_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDL2038777.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIF"
     gene            complement(320..916)
                     /locus_tag="PWY98_003114"
     CDS             complement(320..916)
                     /locus_tag="PWY98_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2038778.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          377 a          195 c          163 g          276 t
ORIGIN      
        1 aaaaaattga aggaatattg tcataagaca aatagtcaat tgcccctcgg tgtaaaataa
       61 aacgggtcgt actgctcaat aagggtaaac aaataaataa aataaagcca tgcatcagca
      121 agacatcacg gaaataagct tgtgttcctt ttaaaaagtc ccaagcatta cctaagctac
      181 gtttcaaata attcatccta tcacctgctc ttcacattgt agcgcaaatc taatgaaaaa
      241 ggaaagggtg cagtgtgttt tgcagaccct ttcccagatt taacattgtc aatcaatgac
      301 taattttaca aaataaatat tatccaagaa gaccacttag atattgacct ccataaatca
      361 aggccatact atataagaaa atcgacagtg gtttagcaat caaaatgatt agtgtatatc
      421 gtttcactga catgtttgtc aatccagcca tcaaacataa cgcatcgtca ggagcaactg
      481 gaagaaaaat agctagagca aacagcttct caaaacggtt ttgattatcc agccagccaa
      541 tatatttgtt atatgtctta tcgctaatca tggaaagtat gaatggcttt ccataacgac
      601 gccctagcag aaaaatgata atcgaaccaa tgcagattcc aatatagtta tagataaatc
      661 cagcataagg gccaaatatc aggacaccag ctgcagtact gatccctcca ggaatgattg
      721 gaataactac ttgcaggatt tggatgaaga caaaaataat cggtcctaaa atgatggaat
      781 cacctactaa tcctcttaac gcattcaaat ctttgaaaac acctaaatta ataaaataaa
      841 tcgtcagcgc gattgatagg cctaatccga taatggatat gaaatttatc agttttcgag
      901 aggcagcaat attcattatt catcaactcc ttagaggttc tttgttaact atactttact
      961 agaaaagcaa ctaactgcgc cattttttga tatgacagaa aacaaacaat t
//
LOCUS       DANKAY010000274         1002 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000274 DANKAY010000000
VERSION     DANKAY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            369..1002
                     /locus_tag="PWY98_003115"
                     /pseudo
     CDS             369..1002
                     /locus_tag="PWY98_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DANKAY010000275         1001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000275 DANKAY010000000
VERSION     DANKAY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
BASE COUNT          352 a          151 c          143 g          355 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga g
//
LOCUS       DANKAY010000276          974 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000276 DANKAY010000000
VERSION     DANKAY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            1..216
                     /locus_tag="PWY98_003116"
     CDS             1..216
                     /locus_tag="PWY98_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038779.1"
                     /translation="MRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQPVTAK
                     KETVFSEGKIKTRYILEVETKFKNRSE"
     gene            222..521
                     /locus_tag="PWY98_003117"
     CDS             222..521
                     /locus_tag="PWY98_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038780.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          364 a          134 c          153 g          323 t
ORIGIN      
        1 attagaaaaa atatcagtga tgaatatgca gacttttatg atcaaagcag taaaaaagat
       61 aaattccaga tggatgtttc aattttagct atattagctt tttccgaaaa taaacaacct
      121 gtaactgcaa aaaaagaaac agtattctct gaaggcaaaa taaaaactcg atatatatta
      181 gaggtagaga ctaagtttaa aaatagatcg gagtaatggt tatgcttttt aatgagacgc
      241 aattatggtt taaatttgat ccttcgaata gatttgtcaa agatttttat aaggtgtggg
      301 attcagaagt tttcttttta gcaatcgaag atagcttatt aattaatctc tactattcta
      361 ataagaacta ctttaaaatc cctgctgcga aaactagaat gaagaaagac gtatattttt
      421 tgtttgatat cgtgactgat gtgctagacg ctcgaagcga tcatcggcgt tatgactata
      481 taaagtatac tttcgttgat ccagaaagat acaaagatta aagtaggcta cctaaaaagg
      541 tagcccggaa cggattttat caccatactt atgaaaggag atatttttta agttagtatt
      601 aagattgtgt aatatgatga tatctatatt ttatagtatc agtgctataa aatcaaaaat
      661 aagtcactaa ttaactacca ctccaattat aagccttttt tctcactttt tttcaaaaat
      721 atggtatgct ttttaatggc ttcaaatata aaagagttta aagcgtaaca cacttatggg
      781 gcagtggttt ttggggaacg ctttaaactc ttctttatta ttatctcaca atataaccca
      841 aatgtctttc tatttaaaaa tcaaagtaaa acttttcaaa tatacagaaa tataactatg
      901 tgaaacatcc tttcattaat ccataaaagg atacataaaa aagccactca tttgagtggc
      961 aatgaagaaa agct
//
LOCUS       DANKAY010000277          961 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000277 DANKAY010000000
VERSION     DANKAY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            172..459
                     /locus_tag="PWY98_003118"
                     /pseudo
     CDS             172..459
                     /locus_tag="PWY98_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="PWY98_003119"
     CDS             473..904
                     /locus_tag="PWY98_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038781.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DANKAY010000278          950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000278 DANKAY010000000
VERSION     DANKAY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            128..>950
                     /locus_tag="PWY98_003120"
     CDS             128..>950
                     /locus_tag="PWY98_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038782.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRK"
BASE COUNT          339 a          135 c          179 g          297 t
ORIGIN      
        1 accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt aatgagcgtt
       61 tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg aattaaacta
      121 tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga tgactaaaaa
      181 agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata tcgaagagat
      241 caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg ggtatcgtct
      301 tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc aaagttctac
      361 aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg agtattattg
      421 tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat
      481 tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa
      541 aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact acggaagacg
      601 cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca agaataattg
      661 tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta ttatgtattt
      721 tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag ctggagactc
      781 ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa atctcttagg
      841 aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata cgattttttc
      901 agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa
//
LOCUS       DANKAY010000279          903 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000279 DANKAY010000000
VERSION     DANKAY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            121..414
                     /locus_tag="PWY98_003121"
     CDS             121..414
                     /locus_tag="PWY98_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2038783.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          345 a          127 c          162 g          269 t
ORIGIN      
        1 aagtagtagt aaaaaaaaga gcactatgat aatatctttc ttaagagtaa ccgctttaat
       61 aaaaaatcca gaatattcta acataaatgg agtattctta gaatcaaaag gagaaagaag
      121 atggtttata cttatacgga gcaagaaaag atgaaagttt tagacacatt catgaatgat
      181 ggaaaagtaa aacaagtacc aagttcaaca aaaaagaaat acatcctatt gaaagaagtc
      241 ttgaaacgct ttgaccatgg agtgacttat acagaaacag aggttaattc gatactgctg
      301 aacgtatttt ctagtggcga ttatgtcgaa caaagacgtt atctaatcac atttggcttt
      361 ttcaaaagaa gttcagacgg tcgggcatat cagatgatgg gaattgaaaa ttagaagttt
      421 taatttatac tcattgaaaa aagttcaagt atgcgatatt ggttctacac cgcatacttg
      481 aacttttttt gtcatctgta tctataatac aaagccaaga acgttattca aaatatggat
      541 agctatgata aaagctaatg agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt
      601 tccaggagcg tacatgaaaa aatcaattgg atgcgttaca tgaataaaga taattctctt
      661 ttatttgtac gaataaatac caaaacgtac ctcctaaaat tagattttgg acagatgccc
      721 tttaaaaaga taacaagatt tcgttcttga aacaatatat atgcttttat tccaatgagg
      781 ttgtgaaaac agtattttgt cctgagcgtt agaaaattga aaatggggaa tagcaactcg
      841 tactttacca ttttttaagt gaatacaaga tcagctagtt gaacaccgtt tattcgtgat
      901 gag
//
LOCUS       DANKAY010000280          895 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000280 DANKAY010000000
VERSION     DANKAY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            252..875
                     /locus_tag="PWY98_003122"
     CDS             252..875
                     /locus_tag="PWY98_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2038784.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          330 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aagaa
//
LOCUS       DANKAY010000281          877 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000281 DANKAY010000000
VERSION     DANKAY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.291"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            126..>877
                     /locus_tag="PWY98_003123"
     CDS             126..>877
                     /locus_tag="PWY98_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2038785.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLL"
BASE COUNT          290 a          112 c          169 g          306 t
ORIGIN      
        1 tattaggaga aaaaacaaat aatactctga aatgtaaaaa ataaatactg actctttacg
       61 cttcagagta tagtacgtac tttcatcaga atgaaaacgc ttaacaacag aagggagaga
      121 attgtatgga aagaaaattt ggtataagag acaaaatagg atatatgttt ggagattttg
      181 gaaacgattt tactttcatt tttgctagtt cttttttaat gatcttttat acaaaagtat
      241 tggggataag cggagctgcc gttggaacat tatttttagt agcaagattt atcgatgcag
      301 ttactgatgt aactatgggg cgtattgttg atagatcgaa agcaactaag cagggtaagt
      361 ttaaaccgtg gattttaaga atgagtggtc cagtcgcttt agcaagtttt ttaatgtatc
      421 aaacttctat gatttcagct tctatgagcg ttaaggtagt atacatgtat gttacgtata
      481 tattatgggg gagtattttt tacacatcta taaatattcc ttatggatca atggcatcag
      541 ctattacttc tattacagaa gaaagaacaa tgctatcgac attccgaaat gttggagcta
      601 ctttagcaag tttggtcatt ggtgtattaa caccattatt tatttataca agagatgatt
      661 caggcgctca aatagttcgt ggtggatcaa cttttacaat tgttgcggga atattttcga
      721 tatgtgcact tatttgttat ttaatatgtt ataaactaac tactgaacga gtaagaattg
      781 accaatctca atacaaagtt tcattgttaa aatctttaaa agggttagtt aaaaataggg
      841 cattactttc tataattggt gcggctattt tcttact
//
LOCUS       DANKAY010000282          845 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000282 DANKAY010000000
VERSION     DANKAY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..141)
                     /locus_tag="PWY98_003124"
     CDS             complement(<1..141)
                     /locus_tag="PWY98_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL2038786.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVR"
     gene            complement(141..740)
                     /locus_tag="PWY98_003125"
     CDS             complement(141..740)
                     /locus_tag="PWY98_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDL2038787.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT          268 a          163 c           93 g          321 t
ORIGIN      
        1 tcttacaatt aactcgtctt tcggatcgta gtcaaagata taatagccta cttcattacc
       61 taaaccacga ttcaataaaa aatcttcttt ttgaattact tcattcaact tttccaaatg
      121 ctcttgaatc gataatgaca ttataaaatt cctcccagtg ctagtacaat atctttacga
      181 ttcttgtcaa ctaaccattg aatcaatcgt ttatctaata atatcggagt cattcgtcca
      241 tattcttttt cccctctgac taatcctgtt tcaattagat tcgtttgata agcggcaata
      301 agccgtttaa tcgtataatc tttccatttt gaaatatctt ctgaacttct ccgcttctct
      361 gtaaaaaaag aacggtaatc aactggtgtt aattgcaaat ttcctttacg tagattttca
      421 gcatatacct cagtcataaa ttcattaaaa agatcatcaa tcataaggat ggaaatcaaa
      481 ttcactaact tttgattatc tatatttaac tgattgaaaa gttgcaatcc agcttcatcc
      541 agttgactaa ctctcctttt cacgttctga tagatttctt tttgacggtt actgctcact
      601 gcaaaaaaaa tattttcatg ttcatttttc tctttaattt ctatatctgt atatccttct
      661 agtcgtaatt gaatatataa cttgaactct gaaaaccaaa agcgtttaga aactagacca
      721 gcagaatatt ttttattcaa tagattgcct cctaatcatc tataattcaa taagtaaaca
      781 agtgtttttt gctcatcgtt agcattcatt ttttagtatc tactatacaa tacatattta
      841 ttata
//
LOCUS       DANKAY010000283          840 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000283 DANKAY010000000
VERSION     DANKAY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.293"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            241..498
                     /locus_tag="PWY98_003126"
     CDS             241..498
                     /locus_tag="PWY98_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038788.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            512..>840
                     /locus_tag="PWY98_003127"
     CDS             512..>840
                     /locus_tag="PWY98_003127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038789.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
BASE COUNT          315 a           97 c          174 g          254 t
ORIGIN      
        1 aaagattgat gcgatcaaga tttattatgt acggagtgcg tattaaaata aaaaatgaac
       61 actcatattt attatacacc gaagaaatag aaaaaaacag actgtttttt taagaaaaac
      121 tatgtcattc tttacttgaa aaaagttgtt ataggacaga aaggataaaa caaaaacgga
      181 aaaaatatgt cttatgaata actataagaa ctattctatt ttttaggaga tggatacgag
      241 atgaaaaaac taattggcta tagtgtgatg tgtattggat ttgttggtgt gggattgttt
      301 gtatcgactg aatctgcttc tgcggctgaa tggtataatc ctatctggta taatccgaac
      361 attgaatgtc aaaaaggcta tccgtatgga ccgacaaatc tgaaaaatgg aaaatgtaga
      421 gtaaattgga atggcgttgt agatagtgta attactggtt caatggactc tgctctacat
      481 ggctttacgg gtcactagaa ggaggaagaa aatgtctgaa gagaaaaaag aaatcgttga
      541 atcattagta gctttaaaag attctctttc taagattgaa tatgttgata ggcaagaaat
      601 tcaaaaacta attgatgata ctattattga aatccaagat gcgcgttgtg aaggtataaa
      661 aatttctgta gctttatcta aagtaataga aaaaatgaac cgttcattag cgtttaatgg
      721 attgaagcta gatagacaaa ctagtctgat atgggatcat ttaaaagact tgtatgacaa
      781 gtctaaaaga agtgaacgga ctgctgtatc aatcttaaaa ggcctttggg gaatgaattc
//
LOCUS       DANKAY010000284          838 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000284 DANKAY010000000
VERSION     DANKAY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..837)
                     /locus_tag="PWY98_003128"
     CDS             complement(<1..837)
                     /locus_tag="PWY98_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038790.1"
                     /translation="MVIQKNNCSLQELSNDLHIPKRTIKELIRKINVSLKQFLLIDEF
                     IYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLE
                     QLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGN
                     DWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITEL
                     MDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFS
                     TSK"
BASE COUNT          276 a          100 c          149 g          313 t
ORIGIN      
        1 tttgcttgtg gaaaataaaa aaccaatctc ctgtttttgt cgaaaagaga tttgatttgg
       61 aaaaaaacaa tagaaaaaat aattaaagta ggctctctct gtttctgtct cttcacaagg
      121 gcgactcact gaaaaagaag taaagtattc tgaataatca tgtgtatgct gcaataagtc
      181 catcaattca gtgatttcag aagaagtttt tggaaaatat gcctgttgtt tgtttcgtaa
      241 tatgaataaa gttaacagca agtagatatt tttttcttgt tttgaaattt tttctatttg
      301 ctcatctgta ctgttcattt ttagtgcatt taattcaagt gaggaaaatg gccaatcgtt
      361 acctccaaat gtatctgaaa gaaaaaagta aagatagaga cggatagata actcatttcc
      421 ttcaagagta cagcaatttt ttacagatag gatccgtaaa tggaattttt ttaaggaatg
      481 attgagctgt tttgtcagtt tttcgagata ggagggcgat atataaagtt gttcaagtaa
      541 atattttttt ggtacagcag tttgtggata attgattagt aacatcaaat agttaaaacg
      601 attggattct tttaggtagc ataattttaa cttagaaaaa acagctaact ttttagtttg
      661 atcggtttct ttggtttgga tctctccttt atgattagaa taaataaatt catcaatcaa
      721 taaaaattgc ttgagtgata catttatttt tcggatcagt tctttgattg tccgttttgg
      781 aatatgtaag tcattggata attcttgtaa agaacaatta tttttttgaa tgaccatt
//
LOCUS       DANKAY010000285          822 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000285 DANKAY010000000
VERSION     DANKAY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..153)
                     /locus_tag="PWY98_003129"
     CDS             complement(<1..153)
                     /locus_tag="PWY98_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2038791.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            234..809
                     /locus_tag="PWY98_003130"
     CDS             234..809
                     /locus_tag="PWY98_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2038792.1"
                     /translation="MKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDDN
                     PTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          259 a          151 c          158 g          254 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DANKAY010000286          816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000286 DANKAY010000000
VERSION     DANKAY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..>816)
                     /locus_tag="PWY98_003131"
     rRNA            complement(<1..>816)
                     /locus_tag="PWY98_003131"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          259 c          181 g          211 t
ORIGIN      
        1 ctctagagtg gtcaaaggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa
       61 accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc aaccttgcgg
      121 tcgtactccc caggcggagt gcttaatgcg ttagctgcag cactgaaggg cggaaaccct
      181 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
      241 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      301 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      361 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      421 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      481 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      541 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      601 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      661 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      721 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      781 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DANKAY010000287          811 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000287 DANKAY010000000
VERSION     DANKAY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..672
                     /locus_tag="PWY98_003132"
     CDS             <1..672
                     /locus_tag="PWY98_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2038793.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DANKAY010000288          792 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000288 DANKAY010000000
VERSION     DANKAY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            10..>792
                     /locus_tag="PWY98_003133"
     CDS             10..>792
                     /locus_tag="PWY98_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2038794.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT          269 a          137 c          153 g          233 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata at
//
LOCUS       DANKAY010000289          784 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000289 DANKAY010000000
VERSION     DANKAY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.299"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
BASE COUNT          280 a           92 c          170 g          242 t
ORIGIN      
        1 gtaacttttt gacaagctca atggcaacac aagcggaaga agtaatacaa gaactaactg
       61 ttatgaataa cagctagata aaatgggcta tgtatgttat ccaacaattt agtaatcact
      121 agaagcctag cgaaagttcg cttgataagt tatagatgag tattgatttg tagtgtttcc
      181 aaatcaaaag ggtacagagc cacatgaaat atgttgtcct ctgcagcgtc aggatgggaa
      241 tccataccat ggagattcgg agatctgtga gggaatactt acgtggttaa tatgtcagtt
      301 gtgttggacg tagtaatggt catagtgggt agattgactg aattcttata tgagaaaatg
      361 aaaccaacaa gaagataggg ttatttatac agaattgggg atcttttctg gaaaagtaat
      421 cggataatgc gagattcgta cggtcttttt taaatttctc taaaaaaaac ggcatcgaat
      481 gggttgggtg attgataagc aatgaatcat agactgatat agaataaaaa atgattctat
      541 ttatcttttt acatctatcg ttttttcaag tagccgcaaa ttatatcagc attcaacaat
      601 ttttgtatac aagttctagt aaaaggatct gaagaaaaag ttggttgttg tgaagataaa
      661 aaaagaatca ggttttactg agtttttctt caaaatttgt gttattaggt taggacaaag
      721 ataagaagaa gtatgaagat ttaaaaaagg aatgaggaaa agatagtaat ttccgattat
      781 tgga
//
LOCUS       DANKAY010000290          748 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000290 DANKAY010000000
VERSION     DANKAY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(109..>748)
                     /locus_tag="PWY98_003134"
     CDS             complement(109..>748)
                     /locus_tag="PWY98_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDL2038795.1"
                     /translation="RRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVT
                     DLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHT
                     DRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQ
                     LRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          209 a          171 c          143 g          225 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgt
//
LOCUS       DANKAY010000291          725 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000291 DANKAY010000000
VERSION     DANKAY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.301"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            28..>725
                     /locus_tag="PWY98_003135"
     CDS             28..>725
                     /locus_tag="PWY98_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2038796.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQA
                     AYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQ
                     CYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYD
                     QLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DANKAY010000292          724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000292 DANKAY010000000
VERSION     DANKAY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..461)
                     /locus_tag="PWY98_003136"
     CDS             complement(<1..461)
                     /locus_tag="PWY98_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2038797.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          233 a          132 c          104 g          255 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
      721 tcct
//
LOCUS       DANKAY010000293          719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000293 DANKAY010000000
VERSION     DANKAY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.303"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            28..696
                     /locus_tag="PWY98_003137"
     CDS             28..696
                     /locus_tag="PWY98_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2038798.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          191 a          124 c          130 g          274 t
ORIGIN      
        1 aaattttcaa aagaaaagag gctagaaatg aaaaaattaa atttatttac agaattgtgc
       61 ggaatcattt taggcgcagg aatctacgga ttagctgtga caggaatcaa tattccatct
      121 aagctggcag atggtggggt tactggtatt gctttgcttt tgcataatct ttttggattc
      181 gctccttctg tcacttcact gctttttaat ttacctttat tgcttatctc attgtttctt
      241 tttggaaaac acgcttttct ccggacgatc gtcggaacat ttgcgctagt tttcttttta
      301 catttatggg agagccttca tatacatttt gctgtagata gtctactgat caatagtttg
      361 atgacaggta ttctttctgg cattggttgc ggtctcgtct ttcgattcgg cggaagcaca
      421 ggcggaacag atatcattta tcaagccatt gaaaaatatt ttcatattaa gattggcaaa
      481 agtcttttct tgattacttt tggtatttta gttgtctcct tgctttattt ggatctcaca
      541 cattttctgt atacattact tagctgttct attctatctt atacattgaa taaagtgaag
      601 tatctggaaa ttggatctgt tttgccccaa ttggaagcaa aacagactac agaaacattt
      661 gaaccacttg atcctcttga acagttagag cactgattct tatttttcac tactttatt
//
LOCUS       DANKAY010000294          707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000294 DANKAY010000000
VERSION     DANKAY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.304"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..598
                     /locus_tag="PWY98_003138"
     CDS             <1..598
                     /locus_tag="PWY98_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2038799.1"
                     /translation="MKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVG
                     GGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKK
                     GQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLV
                     NGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          199 a          139 c          180 g          189 t
ORIGIN      
        1 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
       61 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      121 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      181 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      241 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa aacgcttagg
      301 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      361 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      421 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      481 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      541 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      601 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      661 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DANKAY010000295          670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000295 DANKAY010000000
VERSION     DANKAY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            154..417
                     /locus_tag="PWY98_003139"
     CDS             154..417
                     /locus_tag="PWY98_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2038800.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="PWY98_003140"
     CDS             417..>670
                     /locus_tag="PWY98_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2038801.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DANKAY010000296          670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000296 DANKAY010000000
VERSION     DANKAY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(1..>670)
                     /locus_tag="PWY98_003141"
     CDS             complement(1..>670)
                     /locus_tag="PWY98_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038802.1"
                     /translation="FPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQLEKFS
                     YTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGY
                     KKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNS
                     KNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIR
                     TSHE"
BASE COUNT          245 a           86 c           89 g          250 t
ORIGIN      
        1 tcattcatgg cttgttctta tttttaattt tatgcaataa aaaagaagaa caattagcgt
       61 gattatagaa ataaataatg agattttaga aaaaaatgtg tatgaatacc tgattgttac
      121 tttatttatt ccactttcac tttttactgt aacaaaagga cctccttgtt ttagtactgt
      181 agcatttttt gaattaacag taaccgttaa tcctttatac cggaatacag gtaaaactat
      241 tgttttgcct tttgaagatt taaacgtaaa tgtaatttca tctttatttg gagaaaagct
      301 actataaata acttgaccat cttctgtaac ttgatggttt ttcaaaagca attgatcttt
      361 tttatagcct ataggtacat aatcaaaatt ctcaaattct tcaagtttat tttccatttg
      421 ttcattagaa taagttcctt gaaaattcat ttctttcacc atgttaacta ctgatgaata
      481 atttaaaatt gaaatagcta atattaataa tagtataaaa tttcttctcc ttgtataaga
      541 aaatttttcc aactgtggag aaataaatag agatactgaa aaagatatga acaatgttga
      601 aaatatcata aaacgccaag gagcttgaat aatatttaaa ggagtatttt gtaaggcaaa
      661 ccatggaaat
//
LOCUS       DANKAY010000297          664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000297 DANKAY010000000
VERSION     DANKAY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.307"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            240..638
                     /gene="spxA"
                     /locus_tag="PWY98_003142"
     CDS             240..638
                     /gene="spxA"
                     /locus_tag="PWY98_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2038803.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          237 a           97 c          125 g          205 t
ORIGIN      
        1 gtttcatatg tttagaaaat ttgaattaga tttgaaaact ctaattttta aatgataaaa
       61 aaagcgagaa tgctggtttt actgtatatt aaccttagat aactttcatt tttttaaaaa
      121 aattatgaaa aaaaggcagc tatttaaaag ttgattatgt ataatagaaa cagatgggag
      181 attattatga tcttctgtta caaaaaagtg attcttatca cgaaaaggag tggtgcacgt
      241 tgttaacatt atatacttcc ccaagttgta cttcctgcag aaaggctcgt gcgtggttgc
      301 aagaacatca gattccattt gttgaacgta atattttttc agaaccattg aacagttctg
      361 aattaaaagc gattttacaa atgacagaag acggaacaga agaaatcatc tcgacacgtt
      421 ccaaagtatt ccaaaaactg aacatggatt tagatgatct gccgttacaa gaattattgg
      481 aacttgtgca aaacaatcct ggattgcttc gccgtccaat catgattgat gataaacgtc
      541 tgcaagttgg ttttaatgaa gatgaaattc gtcggttcct accgcgcgat gtacgacaat
      601 tagaattacg tcaagcacaa ttgatggctg gattatagaa ggataatacg aaacttggaa
      661 aaga
//
LOCUS       DANKAY010000298          649 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000298 DANKAY010000000
VERSION     DANKAY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>649
                     /locus_tag="PWY98_003143"
     CDS             <1..>649
                     /locus_tag="PWY98_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038804.1"
                     /translation="EKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWT
                     LDSIFNANFIQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFW
                     NSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLG
                     NGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          240 a           81 c          137 g          191 t
ORIGIN      
        1 aagaaaaacc ttccaacatc tttattatgg ataatccaga tttagaaaag gcaagagaag
       61 tactaagaat gaataaaaat gggattgctt tttctaagaa aggatggcgg gggcctttta
      121 attcagcttg gacattagat agtatattta atgccaattt tatacaaact ggattaatca
      181 aagcagatat ctttcaaaat tcttttaaca agactggaga tgtattgaaa ttagttaatg
      241 gactactcca aatttggaac aacaaaaaga aaataatgga gttaactaaa aaaggaatgg
      301 aattttggaa ttctaagggt tcaattggaa cgattggaac aactgattct gttggtaatc
      361 cttttcctgg ggcttctact ccaaccccta ttgaagataa ttctttagtt attcgtacaa
      421 atggagacgg caaatatatt ttgatttctc ctaaagtagg aaaaggatta gttttattag
      481 gaaatggtaa agcaatttat tttggagact tagatgtaca aggaaaactt acagttaaag
      541 gaaaagaaat aattcctgga caaggtggtg gtccatcggg aggtggagaa tctactggtg
      601 ggtatcctag tgaattaaaa acagatgcag aaaagagagc ttggagaat
//
LOCUS       DANKAY010000299          589 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000299 DANKAY010000000
VERSION     DANKAY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.309"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            290..>589
                     /locus_tag="PWY98_003144"
     CDS             290..>589
                     /locus_tag="PWY98_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2038805.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPT"
BASE COUNT          214 a           67 c           97 g          211 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacc
//
LOCUS       DANKAY010000300          576 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000300 DANKAY010000000
VERSION     DANKAY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.310"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            357..>576
                     /locus_tag="PWY98_003145"
     CDS             357..>576
                     /locus_tag="PWY98_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038806.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLF"
BASE COUNT          152 a           87 c           94 g          243 t
ORIGIN      
        1 tcttaagtaa acttttgttt tggataccat ttatcataat gaccagtgct ttcttttact
       61 tattcaaaag gcctaacaaa tacttactct tatctgcttg tccgatgatc tattttatcc
      121 ataagattag aggattttat tattacgact ctggtatctc attgttttgc tacgaaatcg
      181 gcttattagc ttcggccatc tttttcatat ttgtgttaga atatttcaga aaaaaataaa
      241 cttccagtca gttttggctg gaagtttttt atttggtgca gatcttttca gctgttattc
      301 atacaagttg atccattttt ctattattaa gtacatacca ttgttactct ttgaatgtgg
      361 gggagaaaaa aataaaagga gctggtattt tgttagttat gagtaaagct ttgtttttac
      421 ttccctgcac gttcatgttt attgttttat gttatttcaa aagaagaaaa aaatggtgct
      481 ggatcgatgt tctactattc cttccctttt tcccgcttat ttattttctt gaagaaatat
      541 gggatgatat ggcatgtgat tctgcttttc ttttta
//
LOCUS       DANKAY010000301          552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000301 DANKAY010000000
VERSION     DANKAY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.311"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            188..487
                     /locus_tag="PWY98_003146"
     CDS             188..487
                     /locus_tag="PWY98_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2038807.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          216 a           63 c          103 g          170 t
ORIGIN      
        1 aataaaaatc ttagaataaa aatttttact gatttaattt agaatgtttt taaaataaaa
       61 atcgttgcca taacagtgta ttgaaaggtt ttaacaatcc agtaatgatt taaaaaaatc
      121 tagatgtagc gtatactttg tttatagaat gtagtgggaa gaaatatatt tttgggagga
      181 aatcgtgatg tctaaaagtg aagaaataga aaacatggta ctaaaattat atgatctcgt
      241 tttaaatcca gagatcaaag aaaaggaacg tatacttttg atagacgcaa agacaggctt
      301 agaaaaaggt cagtattatc ctaaagtcat aaataatttg gaacgtagtt tacgtccgtt
      361 agcaatcagg ggagaattgt ctaaaccagt atcaccgttt tatatggaga tatcaacaat
      421 aggaaaattt gaaaaagaat taggtcgagg aatggcttcg gcaccgatta cttttgggca
      481 tttataaaaa cgagttattc atctcaatga attaaaaagt atagtcaatc tcataatgca
      541 ggctaggttc at
//
LOCUS       DANKAY010000302          528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000302 DANKAY010000000
VERSION     DANKAY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.312"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(5..90)
                     /locus_tag="PWY98_003147"
     tRNA            complement(5..90)
                     /locus_tag="PWY98_003147"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="PWY98_003148"
     tRNA            complement(107..178)
                     /locus_tag="PWY98_003148"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="PWY98_003149"
     tRNA            complement(190..262)
                     /locus_tag="PWY98_003149"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="PWY98_003150"
     tRNA            complement(280..361)
                     /locus_tag="PWY98_003150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="PWY98_003151"
     tRNA            complement(369..441)
                     /locus_tag="PWY98_003151"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="PWY98_003152"
     tRNA            complement(445..517)
                     /locus_tag="PWY98_003152"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DANKAY010000303          528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000303 DANKAY010000000
VERSION     DANKAY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.313"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            187..426
                     /locus_tag="PWY98_003153"
     CDS             187..426
                     /locus_tag="PWY98_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2038808.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            475..>528
                     /locus_tag="PWY98_003154"
     CDS             475..>528
                     /locus_tag="PWY98_003154"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2038809.1"
                     /translation="MLVFQMRNVDKTSTVLKQ"
BASE COUNT          219 a           58 c          103 g          148 t
ORIGIN      
        1 ttgtaaaaat tggggaaaag ggatatattg attttatatc tttctcaaaa taaacataaa
       61 agccctttaa agagggcttt tatatattaa tcacaaatca cttatcacaa atcacttatc
      121 acaaatcaca agtgatttgt gattgttgat gataaaataa gaataagaag aaatagaaag
      181 aagtgagtga ttgtgggaaa tttaggcgca caaaaagaaa aacgaaatga tacaccaatc
      241 agtgcaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga cttgcaccat
      301 atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat ggaaataaga
      361 cttagaagca aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg
      421 aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg
      481 gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacag
//
LOCUS       DANKAY010000304          526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000304 DANKAY010000000
VERSION     DANKAY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.314"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..354
                     /locus_tag="PWY98_003155"
     CDS             <1..354
                     /locus_tag="PWY98_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2038810.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="PWY98_003156"
     CDS             355..>526
                     /locus_tag="PWY98_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038811.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DANKAY010000305          523 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000305 DANKAY010000000
VERSION     DANKAY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.315"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            82..495
                     /locus_tag="PWY98_003157"
     CDS             82..495
                     /locus_tag="PWY98_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2038812.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          197 a           84 c           94 g          148 t
ORIGIN      
        1 ttatacttgc atttttttca aaatagagtt attacgtaaa tagggataaa taaaaaatac
       61 ttattgaaag gatgatcaaa aatggcaaat gtacctagca ttcgcgacat gttccctgat
      121 ttcaatgacg tgtttagtcc agcattcaat gattttttag gtgtttcaag ttatccaaaa
      181 gtagatttag tcgaaaatga aaaagaatac aaactaactg ctgatatgcc tggctgtgac
      241 aaagaagaca cgacagttga atattcagat aatactttga cgatctccgc caatcatgag
      301 tcccatacag aagataaaga agatggcaac tatgtacgga aagaaagaca ttcggtctct
      361 tacaaacgtt cattctatct tcctaatgtt gatgaagaaa aaatcactgg aacctttaaa
      421 aacggtgtgc tgaaattggt attacctaaa acagcttatc agccaaaaga aacaaaaaaa
      481 attgagttaa attagaactt atcgcctctc gatgaatgag ggg
//
LOCUS       DANKAY010000306          522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000306 DANKAY010000000
VERSION     DANKAY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.316"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            25..296
                     /locus_tag="PWY98_003158"
                     /pseudo
     CDS             25..296
                     /locus_tag="PWY98_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          197 a           78 c           92 g          155 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggttaaat ctatattttg atatacaaat tacagcttca
       61 gatactaagt tgcataaacc ttttccggac cccatacaaa aggcaattga tgaattgggc
      121 ataaaaaata agaaacgatt tatattggtg attcattata tgacatgcaa agtgcgaaag
      181 cttgtgggat agcatttggt ttagctaaat ggggagccaa aaatatagaa aattttcaaa
      241 gtgtagatat aattattgat tctcctcaag aaatacttga tttgttagaa aaatagacaa
      301 agagcgtgag ataaggatag ggtctcacgc tctttgtttt tcgccattta aagattgaaa
      361 tttttgccat cgccgattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca
      421 ctcaaaatag ttccgaccaa agaattataa ggagtgagta caaatgacct atacccatct
      481 tacaacggat gaacttgtaa taatagagtc ttatttcaaa at
//
LOCUS       DANKAY010000307          521 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000307 DANKAY010000000
VERSION     DANKAY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.317"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..492
                     /locus_tag="PWY98_003159"
     CDS             <1..492
                     /locus_tag="PWY98_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038813.1"
                     /translation="FGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKE
                     TLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKT
                     INSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEY
                     GII"
BASE COUNT          187 a           71 c           82 g          181 t
ORIGIN      
        1 tttgggatag aagaagtttt tgtcggagat aaatgtatct tttttgatca acctttatta
       61 tctgatggtt atggcataga tgaaacagat attttcgcta aagtaaatag tatattttct
      121 ggaaaaaaag aaactttaat taagcttcat cctagagcaa ggacaaatcg ttatggtgaa
      181 aaaaacatat tgaattccag tttaccgttt gaagtcttgc tacttgaaaa tgacttctct
      241 tcaacacttt tgattagtcc tatatctaca atttcttttt caccatctat gatgtttgaa
      301 aagacgatca actctattat gttggctaaa ttaattttaa atgaatatac agcattatct
      361 gaagataaaa aagaaacatt gacaaatatt gttcttttct gtgaacatta taaccgtcta
      421 tcagaagaaa aaattttact cccagaaaac tgggcagaat taagaaagtt ggtagagtat
      481 gggattattt aacacacttt ccaagtcaaa attagttaag t
//
LOCUS       DANKAY010000308          519 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000308 DANKAY010000000
VERSION     DANKAY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.318"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            23..>519
                     /locus_tag="PWY98_003160"
     CDS             23..>519
                     /locus_tag="PWY98_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2038814.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          189 a           95 c           86 g          149 t
ORIGIN      
        1 tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt
       61 cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact
      121 cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa
      181 ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt
      241 aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta
      301 caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac
      361 gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac
      421 catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt
      481 aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DANKAY010000309          514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000309 DANKAY010000000
VERSION     DANKAY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.319"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(32..334)
                     /locus_tag="PWY98_003161"
     CDS             complement(32..334)
                     /locus_tag="PWY98_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038815.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          176 a           90 c           71 g          177 t
ORIGIN      
        1 gttccgggct acctttttta ggtagcctac tttaatcttt atacctttct ggatcaacga
       61 aagtatactt caaatagtca taacgccgat gatcgctccg tgcgtctggc acatcagtca
      121 cgatatcaaa caaaaaatac acatctttct tcattctggt ttttgttgcc ggaattttaa
      181 agtagttctt attagagtaa tagagattga ttaataagct atcttcgatt gctaaaaaga
      241 aaacttctga atcccacacc ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt
      301 taaaccataa ttgtgttttt ccttccatca acataatgtt cacctcaaaa agagtatacg
      361 aactaatgtt ctttttgtaa agtaggaata tcattgataa tatggatgga acatatatat
      421 taaaaagtgt tctttttgaa tgctattttg aatgccattt tcattcattt aacgaaaatc
      481 tgcgaaaata aaaaatgctc aaacccttat aaaa
//
LOCUS       DANKAY010000310          512 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000310 DANKAY010000000
VERSION     DANKAY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.320"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..343
                     /locus_tag="PWY98_003162"
     CDS             <1..343
                     /locus_tag="PWY98_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038816.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DANKAY010000311          500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000311 DANKAY010000000
VERSION     DANKAY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.321"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
BASE COUNT          168 a           96 c           58 g          178 t
ORIGIN      
        1 aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat
       61 tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact
      121 tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc
      181 aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt
      241 agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc
      301 aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa
      361 gacatctcga ttcatattca agataggaat ctttttatca acatcacttt attcgtattt
      421 tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact attcagccca
      481 ttcaacgacc aatgaatttt
//
LOCUS       DANKAY010000312          482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000312 DANKAY010000000
VERSION     DANKAY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.322"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            61..>482
                     /locus_tag="PWY98_003163"
     CDS             61..>482
                     /locus_tag="PWY98_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL2038817.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQV"
BASE COUNT          192 a           59 c           67 g          164 t
ORIGIN      
        1 gtgattagtt agaacttact taaatcctat tataatattt ttgttaatga ttttaataaa
       61 atgattggag agaaaatgat ggatcaaaaa gcgtttatcg aatctattga ttctacaatc
      121 gatagattaa aaaaagaaat tcgattttat aatagagtaa ttggtatcgg aaatattata
      181 aaaatagtct tatcagctag tatcccaata ttaattgatc aggcttccga acacaggtct
      241 ttactattga ttgtttcaat tgcttcagca ataattacaa ttatacaaag tgggatgtct
      301 gcatttaatt atcaagataa agtacagact tcgacagagt tattaatgaa aattgaaaaa
      361 gaaaagttgt tatatataac gaaaacatcc ccatataata aaacagatga agaaaatttc
      421 catttaatcg ttacaaccct tcaaactgaa ttaaatgata ttatctctga ttttaatcaa
      481 gt
//
LOCUS       DANKAY010000313          476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000313 DANKAY010000000
VERSION     DANKAY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.324"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            22..>476
                     /locus_tag="PWY98_003164"
     CDS             22..>476
                     /locus_tag="PWY98_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2038818.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DANKAY010000314          468 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000314 DANKAY010000000
VERSION     DANKAY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.325"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>468
                     /locus_tag="PWY98_003165"
     CDS             <1..>468
                     /locus_tag="PWY98_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2038819.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DANKAY010000315          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000315 DANKAY010000000
VERSION     DANKAY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.326"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..430)
                     /locus_tag="PWY98_003166"
     CDS             complement(<1..430)
                     /locus_tag="PWY98_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2038820.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANKAY010000316          430 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000316 DANKAY010000000
VERSION     DANKAY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.327"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>430
                     /locus_tag="PWY98_003167"
     CDS             <1..>430
                     /locus_tag="PWY98_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL2038821.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DANKAY010000317          421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000317 DANKAY010000000
VERSION     DANKAY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.328"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(170..355)
                     /locus_tag="PWY98_003168"
     CDS             complement(170..355)
                     /locus_tag="PWY98_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HDL2038822.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DANKAY010000318          420 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000318 DANKAY010000000
VERSION     DANKAY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.329"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            74..403
                     /locus_tag="PWY98_003169"
     CDS             74..403
                     /locus_tag="PWY98_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2038823.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DANKAY010000319          418 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000319 DANKAY010000000
VERSION     DANKAY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.330"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(45..>418)
                     /locus_tag="PWY98_003170"
     CDS             complement(45..>418)
                     /locus_tag="PWY98_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2038824.1"
                     /translation="YEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTD
                     VNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNY
                     NIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          152 a           64 c           22 g          180 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatact
//
LOCUS       DANKAY010000320          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000320 DANKAY010000000
VERSION     DANKAY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.331"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..339)
                     /locus_tag="PWY98_003171"
     CDS             complement(<1..339)
                     /locus_tag="PWY98_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038825.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DANKAY010000321          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000321 DANKAY010000000
VERSION     DANKAY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.332"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>404
                     /locus_tag="PWY98_003172"
     CDS             <1..>404
                     /locus_tag="PWY98_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038826.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DANKAY010000322          403 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000322 DANKAY010000000
VERSION     DANKAY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.333"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..376
                     /locus_tag="PWY98_003173"
     CDS             <1..376
                     /locus_tag="PWY98_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDL2038827.1"
                     /translation="IDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQF
                     TGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFG
                     KLIKAVHELYMKKGYENKTKRM"
BASE COUNT          157 a           72 c           65 g          109 t
ORIGIN      
        1 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
       61 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
      121 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
      181 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
      241 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
      301 atttggcaag ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa
      361 aacaaagagg atgtgacaaa attcttgtca catcctctta ttc
//
LOCUS       DANKAY010000323          389 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000323 DANKAY010000000
VERSION     DANKAY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.334"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(131..>389)
                     /locus_tag="PWY98_003174"
     CDS             complement(131..>389)
                     /locus_tag="PWY98_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038828.1"
                     /translation="ELSEGKTGLANILNFLKEIVHIDVSKISLMELTNTHIKEENVPL
                     FVFETEQKNQREELGEGYIWEEPGQMRSVLGTYEVEGVPFF"
BASE COUNT          131 a           75 c           45 g          138 t
ORIGIN      
        1 aacatatttt gaaaataaaa tgcactaaaa aactatcttt agcagaacat ttcattgtat
       61 tttttggacc aaacaataaa aaaagaagtt tgaaaaaaat tcaaacttct ttgttcgctt
      121 aatctgtatt ttagaagaaa ggcactccct ctacttcgta agttccaaga acagagcgca
      181 tttgacctgg ttcttcccaa atatatcctt cgcctaattc ttctctttga ttcttttgtt
      241 cagtttcaaa gacaaacaac ggaacatttt cctctttaat atgtgtattt gtcaattcca
      301 tcaaactaat cttgctaaca tcgatatgaa ctatttcttt taagaaatta agtatattcg
      361 ctaaaccagt ctttccttca gaaagttcc
//
LOCUS       DANKAY010000324          386 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000324 DANKAY010000000
VERSION     DANKAY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.335"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>386
                     /locus_tag="PWY98_003175"
     CDS             <1..>386
                     /locus_tag="PWY98_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2038829.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DANKAY010000325          376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000325 DANKAY010000000
VERSION     DANKAY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.337"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            134..307
                     /locus_tag="PWY98_003176"
     CDS             134..307
                     /locus_tag="PWY98_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038830.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          118 a           49 c           51 g          158 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc atttta
//
LOCUS       DANKAY010000326          363 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000326 DANKAY010000000
VERSION     DANKAY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.338"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>363
                     /locus_tag="PWY98_003177"
     CDS             <1..>363
                     /locus_tag="PWY98_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDL2038831.1"
                     /translation="GIFVGLLTGIVVSLFRLSIEKLSDLVRSIYEMSREQPIYLIGLA
                     ICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSMNWFSVLWKKFIGGVLSVGAG
                     LFLGREGPSIQLGASIGQG"
BASE COUNT           92 a           64 c           96 g          111 t
ORIGIN      
        1 ggaatatttg ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa
       61 aagctaagcg atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta
      121 attggcctag ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat
      181 gaaccggata tcaagggaag cgggattcca caagtagtgg gacagcttcg cggggaatta
      241 tcaatgaact ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt
      301 gcgggccttt ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa
      361 gga
//
LOCUS       DANKAY010000327          323 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000327 DANKAY010000000
VERSION     DANKAY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.339"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWY98_003178"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWY98_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2038832.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DANKAY010000328          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000328 DANKAY010000000
VERSION     DANKAY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.340"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>321
                     /locus_tag="PWY98_003179"
     CDS             <1..>321
                     /locus_tag="PWY98_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2038833.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DANKAY010000329          315 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000329 DANKAY010000000
VERSION     DANKAY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.341"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..>315
                     /locus_tag="PWY98_003180"
     CDS             <1..>315
                     /locus_tag="PWY98_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2038834.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DANKAY010000330          309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000330 DANKAY010000000
VERSION     DANKAY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.denovo.342"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            1..309
                     /locus_tag="PWY98_003181"
                     /pseudo
     CDS             1..309
                     /locus_tag="PWY98_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DANKAY010000331         4802 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.159, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000331 DANKAY010000000
VERSION     DANKAY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            90..785
                     /gene="vanR"
                     /locus_tag="PWY98_003182"
     CDS             90..785
                     /gene="vanR"
                     /locus_tag="PWY98_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2038835.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="PWY98_003183"
     CDS             763..1917
                     /locus_tag="PWY98_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2038836.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="PWY98_003184"
     CDS             2132..3100
                     /locus_tag="PWY98_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2038837.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="PWY98_003185"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="PWY98_003185"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2038838.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="PWY98_003186"
     CDS             4130..4738
                     /locus_tag="PWY98_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL2038839.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DANKAY010000332         4245 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.171, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000332 DANKAY010000000
VERSION     DANKAY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            242..877
                     /locus_tag="PWY98_003187"
     CDS             242..877
                     /locus_tag="PWY98_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038840.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1031..1108
                     /locus_tag="PWY98_003188"
                     /pseudo
     CDS             1031..1108
                     /locus_tag="PWY98_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1353..1469
                     /locus_tag="PWY98_003189"
     CDS             1353..1469
                     /locus_tag="PWY98_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2038841.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            1485..3404
                     /gene="tet(M)"
                     /locus_tag="PWY98_003190"
     CDS             1485..3404
                     /gene="tet(M)"
                     /locus_tag="PWY98_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDL2038842.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            3523..3690
                     /locus_tag="PWY98_003191"
     CDS             3523..3690
                     /locus_tag="PWY98_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2038843.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(3750..4103)
                     /locus_tag="PWY98_003192"
     CDS             complement(3750..4103)
                     /locus_tag="PWY98_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2038844.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         1458 a          641 c          835 g         1311 t
ORIGIN      
        1 aatactttgt cagcaagttc tgttttgcct tttctaacaa gttccatttc aaccgatatt
       61 catatctacg gatttaatca gataggtatt tctttttgaa tgtttttata tttcattcta
      121 ttgtgcttat tcgattacaa gaacatagta attcttatat gcttatttgt caatatcttt
      181 ttttgccttt acaaatcata ttaagcatga tagacttata atagcgaaga tataacgaga
      241 tatggagggt tatgcaatga aaataagcca tttaggaaat aatatacaga ccataagaaa
      301 atttagggga atgaaacaac aggaacttgc ggacaaaatc ggtatcaata tgcagagcct
      361 ttccaagatt gaaagagggt tgaactatcc tgcctatgaa acgctggaaa agataatgga
      421 agtgctggac gttacgccca atgaattgtt gtcgggagaa tggaagtatg tcaatcaatc
      481 tgaaaaggaa gtctgtcagt ttttaagagc cgaagaacgt ctaaatgcag aactgaaaca
      541 tggacactat gataacttct ttgacagcga ggaagaatgg ctggaatatg agttagaaaa
      601 gttacgggaa tatattacag actatatcaa cgggaaaagc attgaagcgt ccgaccttta
      661 cccaatcaaa gaatttatcc agcatttgaa gtttcaaaaa ttattagacc gctatgatga
      721 tttatacagt atggatatgt ttggggaaag catagaggga cataagtata agacacctta
      781 tcaagtcgtg aaaatgataa acccgaactc aaaagaagat atggaactgt tacgagaagt
      841 attaaggaat aacaattttg atgatgagga cgagtaactt ttttcctctt gcgagaagat
      901 acagcaaaca aaaagcccga tcaagagtgc aaagcaccac caatggtggc aaagctcttg
      961 acagggcttt ttcttttctg ttgtgggcaa tcaagaggaa gaaagggata aaatctgatt
     1021 tgtggtaaat caggcaaagg caacgcaggt atctcaattt gatttggtac ttgaaaagaa
     1081 cgggagtaat tggaagatta tagaataaca aatattggta cattattaca gctattttgt
     1141 aatcacgtac tctctttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc
     1201 tattatttaa gtatctattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata
     1261 aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc ttttttagga gggcttagtt
     1321 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatccg gagaatatct
     1381 gtatgctttg tatgcctatg gttatgcata aaaatcccag tgataaaagt atttatcact
     1441 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
     1501 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
     1561 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
     1621 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
     1681 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
     1741 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
     1801 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
     1861 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
     1921 agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg
     1981 tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt
     2041 tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca
     2101 taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag
     2161 ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc
     2221 cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg
     2281 catatatacg cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa
     2341 aagaaaaaat aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg
     2401 atagagctta ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg
     2461 ttcttggaga tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac
     2521 tacaaacaac tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt
     2581 tggaaatctc agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa
     2641 ttatactttc tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa
     2701 agtatcatgt ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa
     2761 aaaatgcaga atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg
     2821 gtttatctgt atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc
     2881 ttggatactt aaatcaatcg tttcaaaatg cagttatgga ggggatacgc tatggctgtg
     2941 aacaaggatt gtatggttgg aatgtgacgg actgtaaaat ctgttttaag tatggcttat
     3001 actatagccc tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac
     3061 aagtcttaaa aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg
     3121 cgccacagga atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg
     3181 tagacactca attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta
     3241 ttcaagaata tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag
     3301 agttaaaagg gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata
     3361 gtcggataga taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt
     3421 atataaatat ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa
     3481 agtgtaaagg aggagatagt tattataaac tacaagtgga tattgtgtcc tgtatgtgga
     3541 aataaaacac gattaaagat aagggaagat actgaattaa aaaaattccc cctctattgt
     3601 ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac tgtgattaca
     3661 gagccagacg caaagacgca gagccgataa aatgagatta atacaatctc attttatcgg
     3721 ctctttccgt tatgtatgga ttcttttaat tagtcttcga tgtttcttgc ttcgttgata
     3781 ccgctggcta aagattccat taaggatagt tctttgtctg taaagctatc catgtatttc
     3841 tctatctgta atcgtcgggt gctttttacc aagttattag caggtaagaa aaattcatca
     3901 acggaaacat gaagtaacga tacaaggtca taaagaactt gtatgctggg gtgttgccct
     3961 ttattttcaa tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca
     4021 cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc tctaaaatca
     4081 tatttatctt cttttttacg catagtagac cacctctata cattttattg ttcctactga
     4141 attaaaaaca ggtatagaaa aacgtgttat atggtttata ggtttatatt taataaaaag
     4201 cactactaaa cgccaataaa aaaaaccgtt atatggtagt gctat
//
LOCUS       DANKAY010000333         3566 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.184, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000333 DANKAY010000000
VERSION     DANKAY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            103..1224
                     /locus_tag="PWY98_003193"
     CDS             103..1224
                     /locus_tag="PWY98_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2038845.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1482..2984
                     /gene="eat(A)"
                     /locus_tag="PWY98_003194"
     CDS             1482..2984
                     /gene="eat(A)"
                     /locus_tag="PWY98_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2038846.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3051..>3566
                     /locus_tag="PWY98_003195"
     CDS             3051..>3566
                     /locus_tag="PWY98_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2038847.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELV"
BASE COUNT         1222 a          615 c          804 g          925 t
ORIGIN      
        1 gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa
       61 tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca
      121 attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc
      181 tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact
      241 gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc
      301 ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca
      361 gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact
      421 atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa
      481 gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta
      541 ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact
      601 aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct
      661 gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa
      721 aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag
      781 caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc
      841 ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc
      901 agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa
      961 gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca
     1021 aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga
     1081 ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa
     1141 gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga
     1201 tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc
     1261 aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg
     1321 cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg
     1381 gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt
     1441 tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa
     1501 aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa
     1561 attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt
     1621 tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat
     1681 ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg
     1741 atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc
     1801 ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac
     1861 tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt
     1921 tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca
     1981 tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg
     2041 aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac
     2101 ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa
     2161 agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga
     2221 aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg
     2281 atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca
     2341 gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc
     2401 aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc
     2461 tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc
     2521 ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg
     2581 ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg
     2641 aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat
     2701 ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc
     2761 agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg
     2821 aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag
     2881 aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc
     2941 tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag
     3001 aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg
     3061 taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg
     3121 tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt
     3181 tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac
     3241 aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg
     3301 attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag
     3361 cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc
     3421 ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg
     3481 ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat
     3541 tcatggtaga taatacagaa ttggtt
//
LOCUS       DANKAY010000334         2528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.208, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000334 DANKAY010000000
VERSION     DANKAY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            4..333
                     /locus_tag="PWY98_003196"
     CDS             4..333
                     /locus_tag="PWY98_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2038848.1"
                     /translation="MNVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNTVNGLN
                     QQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTADKSQQI
                     VEEHLRK"
     gene            660..1157
                     /gene="dfrG"
                     /locus_tag="PWY98_003197"
     CDS             660..1157
                     /gene="dfrG"
                     /locus_tag="PWY98_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2038849.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1229..>2528)
                     /locus_tag="PWY98_003198"
     CDS             complement(1229..>2528)
                     /locus_tag="PWY98_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2038850.1"
                     /translation="FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYE
                     TQPLQTKKFNEMWLNTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRY
                     VFRSDVAYDWNISMDELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNA
                     YLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYR
                     VFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASH
                     YLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNE
                     IAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITIL
                     RTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          889 a          403 c          434 g          802 t
ORIGIN      
        1 gaaatcaatg tcaaactaga tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat
       61 gcagattgga cagcaagact acaagaagca caagcaaaac tttcagaatt acaaaatact
      121 gtaaatggtt tgaatcaaca aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact
      181 cagttaacag atacacaaaa gaaattagac gaagcaactg ctgaaaactc aagcttgaaa
      241 caatatattc aaaagttaga aaaagcaaaa gatgaagtgg aagatacagc tgataaatct
      301 cagcaaattg ttgaagaaca tttgcgtaaa taatctttct taacaagtaa acctaaatta
      361 gtaaagaagc aacagtaatc tatactaagt agttcagttt tggagtacaa aatgacaaaa
      421 tgtacttcaa aatggactac ttttttcatt ttaacttaca taccaaatta catacaaaat
      481 gatttataaa atgcaattga aaatgataat tgaatgaaaa tttgtgattt tgtggtatta
      541 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
      601 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
      661 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
      721 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
      781 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
      841 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
      901 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
      961 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
     1021 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
     1081 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
     1141 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
     1201 agcttgggat tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa
     1261 attgtttatc tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt
     1321 taaaagggcg acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact
     1381 tcggattgaa gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc
     1441 tttcaagaga tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat
     1501 taacatttaa tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac
     1561 aatcaactga ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg
     1621 catatttgcg atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg
     1681 ttagtaattc agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc
     1741 tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt
     1801 ttctgctttt cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt
     1861 ctttcgtgaa tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc
     1921 gccattcgtc ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag
     1981 gagcatcaac atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt
     2041 gaagataagc attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc
     2101 aatctaacct atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg
     2161 tatcttcatt taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca
     2221 catatcttga taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa
     2281 accccgtata tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca
     2341 tcttgtccgt attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat
     2401 atcgttctaa aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc
     2461 caactccaat tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct
     2521 tagcgaac
//
LOCUS       DANKAY010000335         2480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.211, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000335 DANKAY010000000
VERSION     DANKAY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            260..1171
                     /locus_tag="PWY98_003199"
     CDS             260..1171
                     /locus_tag="PWY98_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL2038851.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ"
                     /locus_tag="PWY98_003200"
     CDS             1324..1809
                     /gene="vanZ"
                     /locus_tag="PWY98_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL2038852.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..>2480
                     /locus_tag="PWY98_003201"
     CDS             2328..>2480
                     /locus_tag="PWY98_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2038853.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIA"
BASE COUNT          899 a          359 c          463 g          759 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca
//
LOCUS       DANKAY010000336         2479 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.212, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000336 DANKAY010000000
VERSION     DANKAY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            52..1530
                     /gene="msr(C)"
                     /locus_tag="PWY98_003202"
     CDS             52..1530
                     /gene="msr(C)"
                     /locus_tag="PWY98_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2038854.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1577..2023
                     /locus_tag="PWY98_003203"
     CDS             1577..2023
                     /locus_tag="PWY98_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2038855.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2222..>2479)
                     /locus_tag="PWY98_003204"
     CDS             complement(2222..>2479)
                     /locus_tag="PWY98_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2038856.1"
                     /translation="QKDEFLAKIYAFSTLDKTTATGLLFTREQQLNKIMQKNQKKLND
                     LSLDKKQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQFFE"
BASE COUNT          795 a          418 c          519 g          747 t
ORIGIN      
        1 ttttttagta tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat
       61 ttagcagtaa atataacaaa tctacaagtc agctttggca accagctaga actatctatt
      121 gattctcttc gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt
      181 aaatcgactt tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa
      241 acagagatca ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta
      301 aatttggaat tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg
      361 agcggagggg aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg
      421 atgcttctag acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa
      481 gagcttcgtt attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat
      541 gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat
      601 tatagtgcct atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag
      661 tcgatcatga aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg
      721 gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagact ggatcggttg
      781 tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta
      841 gagagaagac tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt
      901 tttccagtac caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc
      961 caattggaaa gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt
     1021 aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc
     1081 cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa
     1141 aagggttatc aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag
     1201 ttttcagaat cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg
     1261 acgaaaccgt tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt
     1321 tttactaagc caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca
     1381 acgatcgaag ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct
     1441 catgattctt actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa
     1501 ataaaaaaag tacttacgag aaatttttaa atgtgctacg atgattttcg tgacaaattt
     1561 aaaggagtgg tacagattgc ctacaaataa aaaagaagga ataattttta caacaatgat
     1621 gtgttttttg atggtcttgg ggatgagcgc gtataattta tggctgcatc aagatcttca
     1681 tttatcaagt ttgttgattg gtctagtgcc tggattcgtg gtggctttca ttttggatgt
     1741 cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa atccagaaag
     1801 caaggtccaa atgatcttaa ctatttcatg cttgatggtt ttaggaatgg tgacatgtat
     1861 gtctttattc ggtgtgatca tggaacaagg aatacctgat caattattct ctgcttatat
     1921 gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg cagttgatca tcgttggccc
     1981 gctttcaaga agtgtgctaa aagcaattca aagtaatgaa tgatggaaca ctgttatttg
     2041 aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa tgcagttcta gaaaaatagt
     2101 gatccgaacg ataggttttg gcctgtcgtt cggatcgctt tttcttttcc aactgtattg
     2161 ctcttttatt gaagataatt tgctcttcaa tagatatttc tagaaaggtt ctgtttatcc
     2221 cttactcaaa aaactgctgc atttgttctc ttacttgctg acaccattgg agttcctgct
     2281 gacaaagcat aattctgcgc tggatcacaa cataacgtcc aaattcttgt ttcttgtcta
     2341 gcgagaggtc attcaatttt ttttgatttt tttgcatgat tttgttcagc tgttgttctc
     2401 tagtgaatag cagtccagta gctgttgttt tatcgagcgt cgaaaaagca tagatttttg
     2461 ctagaaattc atctttttg
//
LOCUS       DANKAY010000337         2385 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.215, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000337 DANKAY010000000
VERSION     DANKAY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            <1..179
                     /locus_tag="PWY98_003205"
     CDS             <1..179
                     /locus_tag="PWY98_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2038857.1"
                     /translation="EYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEIN
                     NFLNEESSDERKQI"
     gene            160..894
                     /locus_tag="PWY98_003206"
     CDS             160..894
                     /locus_tag="PWY98_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2038858.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            927..1835
                     /locus_tag="PWY98_003207"
     CDS             927..1835
                     /locus_tag="PWY98_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2038859.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1832..2312
                     /locus_tag="PWY98_003208"
                     /pseudo
     CDS             1832..2312
                     /locus_tag="PWY98_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          834 a          337 c          531 g          683 t
ORIGIN      
        1 ttgaatataa acctgaaaaa tatgccgaaa gagttaatca tatttttgaa gtactcggtc
       61 tttctctttt tgaatgctac gatatgaccg agaaacttta taaagaagtg aatgaaattg
      121 tatcggagat aaataacttt ttaaacgagg agagttcaga tgaaagaaaa caaatatgat
      181 gataatatat tttttcaaaa atacagtcaa atgagtcgct cgcagaaagg actggctggt
      241 gcgggagaat gggagacttt gaaaaagatg ctacctgatt ttaagggtaa gcgtgtgctt
      301 gatttaggat gcggctatgg atggcactgt atatatgcga tggaaaacgg tgcttcctct
      361 gtagtaggtg ttgatatttc tcataaaatg ctcgaagtag caaaaggaaa aacccatttt
      421 ccacagattg aatatgaatg ctgtgccata gaagatgtgg atttcccaga ggagagcttt
      481 gatgtaatac taagttcgct tgcgtttcat tatgtagcag actatgagaa tttaataaaa
      541 aagatatata ggatgctgaa ggctggtggc aatttagttt ttacagttga acatcctgtt
      601 tttactgctc atggaacaca agactggtat tataacgaaa aaggagaaat actgcatttc
      661 ccggtggaca attattatta tgagggcaaa cggacagcta tgtttttgga agaaaaggtt
      721 acaaaatatc atagaacact gaccacatat ctaaatacac tgctttcaaa tagttttata
      781 ataaatcaga ttgtggagcc acagccgcca gagaacatga tggatattcc ggggatggcg
      841 gatgaaatgc gacgcccaat gatgctgatt gtatcggcaa aaaagaagat gtaataatat
      901 agaaaaaata aacgaggagt atgtaaatga gatcagaaaa agaaatgatg gatttagtac
      961 tttctttagc agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
     1021 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
     1081 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
     1141 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
     1201 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
     1261 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
     1321 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
     1381 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
     1441 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
     1501 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
     1561 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
     1621 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
     1681 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
     1741 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
     1801 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
     1861 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     1921 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     1981 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     2041 tgataagata atatatcttt tatatagaag atatcgccgt atgtaaggat ttcagggggc
     2101 aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc
     2161 atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt
     2221 gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2281 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2341 tcttgtggtt ctgttgcaaa gttttaaata aagaataaaa tccct
//
LOCUS       DANKAY010000338         1710 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.238, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000338 DANKAY010000000
VERSION     DANKAY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            5..652
                     /locus_tag="PWY98_003209"
     CDS             5..652
                     /locus_tag="PWY98_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HDL2038860.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1345..1710)
                     /locus_tag="PWY98_003210"
                     /pseudo
     CDS             complement(1345..1710)
                     /locus_tag="PWY98_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          586 a          223 c          281 g          620 t
ORIGIN      
        1 atatatgact tttaatatta ttgaattaga aaattgggat agaaaagaat attttgaaca
       61 ctattttaat cagcaaacta cttatagcat tactaaagaa attgatatta ctttgtttaa
      121 agatatgata aaaaagaaag gatatgaaat ttatccctct ttaatttatg caattatgga
      181 agttgtaaat aaaaataaag tgtttagaac aggaattaat agtgagaata aattaggtta
      241 ttgggataag ttaaatcctt tgtatacagt ttttaataag caaactgaaa aatttactaa
      301 catttggact gaatctgata aaaacttcat ttctttttat aataattata aaaatgactt
      361 gcttgaatat aaagataaag aagaaatgtt tcctaaaaaa ccgatacctg aaaacaccat
      421 accgatttca atgattcctt ggattgattt tagttcattt aatttaaata ttggtaacaa
      481 tagcagcttt ttattgccta ttattacgat aggtaaattt tatagtgaga ataataaaat
      541 ttatatacca gttgctctgc aacttcatca ttctgtatgt gatggttacc atgcttcact
      601 atttatgaat gaatttcaag atataattca tagggtagat gattggattt agtttttaga
      661 ttttgaaagt gaatttaatt ttatacacgt aagtgatcat aaaatttatg aacgtataac
      721 aaccacattt tttggttgct tgtggttttg attttgaatt tggttttgaa cttatggact
      781 gatttattca gtccattttt tgtgcttgca caaaaactag cctcgcagag cacacgcatt
      841 aatgacttat gaaacgtagt aaataagtct agtgtgttat actttacttg gaagatgcac
      901 cgaataaaaa atattgaaga acaactagca aaagatttta aagagttatt ttattttaag
      961 tctttataac atgagtgaag cgaattttta aatttcgata gaaattttta catcaaaaag
     1021 ccccctgtca aaattgacga agggggtttt ttggcgcacg cttttcgtta gaaatataca
     1081 agattgaaaa tcgtgtataa gtgcgccctt tgttttgaac ttagcacgtt acatcaattt
     1141 tttaaaatga tgtataagtg cgccctttta aattttgagt gattatattt tttgagttag
     1201 aaaaagggat tgggaaaatt tcccaaaata atttaaaaaa taagcaaaaa ttttcgatag
     1261 agaatgtgct attttttgtc aaaggtgtat accttgactg tgcttgctgt tacattaagt
     1321 ttatttttaa gttattaaaa aagaaatagc ttttaaagtt tggctcgctg tcgctttata
     1381 aagctgattg acttttgatt gcaaactact taaagaaaac aaactcggac tattcgtttt
     1441 cttctctttg gtttgaacat cagcaattat cccctcttga ttgcctattt tagcttgttt
     1501 agaagaaaca aaagctaaaa gctcctcttg ggttttaaaa cgctgtgtgg ggcttagaac
     1561 gcccttaaac gacccttggt ttacttttat actagcttcc acctcgaaaa aaggttcttt
     1621 tttaaaattc tctatggctt cctggcgctg aaaaataagg tataaggtgg gcgtttgaac
     1681 acgtcctagt gaaaatgtac cttgtacgcc
//
LOCUS       DANKAY010000339         1147 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.270, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000339 DANKAY010000000
VERSION     DANKAY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            10..75
                     /locus_tag="PWY98_003211"
     CDS             10..75
                     /locus_tag="PWY98_003211"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2038861.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            200..937
                     /gene="erm(B)"
                     /locus_tag="PWY98_003212"
     CDS             200..937
                     /gene="erm(B)"
                     /locus_tag="PWY98_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2038862.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          420 a          199 c          185 g          343 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcga aacagactaa aaacagtgat
       61 tacgcagata aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt
      121 aaacagataa ctaaaattac aaacaaatcg tttaacttct gtatttgttt atagatgtaa
      181 tcacttcagg agagattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag
      241 tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta
      301 cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt
      361 aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa
      421 tactcgtgtc actttaattc accaagatat tctacagttt caattcccta acaaacagag
      481 gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt
      541 ttttgaaagc cgtgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac
      601 cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt
      661 gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa
      721 acttacccgc cataccacag atgttccaga taaatattgg aagctatata cgtactttgt
      781 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
      841 aatgaaacac gccaaagtaa acaatttaag taccattact tatgagcaag tattgtctat
      901 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttttaaat
      961 ttggaaagtt acacgttact aaagggaatg gagataaatt attagatata ctactgacag
     1021 cttccaagaa gctaaagagg tccctagcgc ctacggggaa tttgtatcga taaggggtac
     1081 aaattcccac taagcgctcg ggaccccttg taggaaaatg tcctaagtgt ggcaacaata
     1141 ttgtatt
//
LOCUS       DANKAY010000340          903 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538824-rid17233803.guided.289, whole genome shotgun
            sequence.
ACCESSION   DANKAY010000340 DANKAY010000000
VERSION     DANKAY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538824
            Sequence Read Archive: ERR10688387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638148.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638148.1
            Genome Coverage       :: 51x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:40:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,149
            Genes (coding)                    :: 3,070
            CDSs (with protein)               :: 3,070
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 36 of 79
            Pseudo Genes (incomplete)         :: 37 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 11 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538824-rid17233803.guided.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2017-08-09"
     gene            187..735
                     /gene="aac(6')"
                     /locus_tag="PWY98_003213"
     CDS             187..735
                     /gene="aac(6')"
                     /locus_tag="PWY98_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2038863.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          317 a          135 c          196 g          255 t
ORIGIN      
        1 ccaattgatg gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt
       61 tattttataa gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt
      121 ttgagcgaga acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga
      181 agacgtatga taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct
      241 gatttactga gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa
      301 gaaatgatga atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga
      361 tttattggtg caatccctca atacggtatc acaggttggg aattgcatcc attagttgta
      421 gaaagctccc gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa
      481 gtagcttcca gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca
      541 acgttaagtc aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac
      601 cttcgtgaac atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta
      661 ccaaatgcaa atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga
      721 ccaaattctc aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa
      781 ggaatttacg aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc
      841 tacttttgga acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag
      901 aaa
//
